Applied Microbiology and Biotechnology最新文献

{"title":"Phosphoproteomics analysis of acid stress response of Alicyclobacillus acidoterrestris in response to acid stress.","authors":"Yingli Liu, Kunrun Wu, Lingxia Jiao, Rui Shen, Junjian Ran, Youzhi Wu","doi":"10.1007/s00253-025-13525-y","DOIUrl":"10.1007/s00253-025-13525-y","url":null,"abstract":"<p><p>Alicyclobacillus acidoterrestris causes pasteurized acidic juices spoilage, resulting in a significant decline in juice quality and causing economic losses. Exploration of A. acidoterrestris in response to acid stress could help control contamination caused by the bacteria. In this study, the mechanism of A. acidoterrestris in response to acid stress was studied by quantitative phosphoproteomics technique. Results showed that the phosphorylation of 40 proteins in A. acidoterrestris was closely related to the regulation of acid stress. The KEGG pathway enrichment analysis showed that the quorum sensing pathway, which might be involved in the perception of A. acidoterrestris, was mainly enriched. We found that the upregulation of Spo0A and YidC phosphorylationmay resist acid stress by forming spores. The phosphorylation level of pyruvate kinase increased, which may improve bacterial acid stress resistance through the formation of energy supply. The phosphorylation level of ABC transporter permease was significantly upregulated, which may be part of the cell adaptation adjustment and contribute to the survival of A. acidoterrestris under acid stress. In summary, the molecular mechanism of acid stress regulation of A. acidoterrestris was proposed via quantitative phosphoproteomics, which provided a theoretical and experimental basis for further investigation of the acid resistance mechanism of A. acidoterrestris. KEY POINTS: • Spo0 A, Yidc proteins may be the key regulatory proteins for acid stress response. • ABC transporters are beneficial to the survival of the bacteria under acidic stress. • A. acidoterrestris may sense and transmit pH signal through quorum sensing pathway.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"141"},"PeriodicalIF":3.9,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergent roles of the acetyl-CoA synthetases RkACS1 and RkACS2 in carotenoid and lipid biosynthesis in Rhodosporidium kratochvilovae.","authors":"Meixia He, Xiaoxia Yang, Chao Xiong, Yuxuan Gan, Hongjun Ma, Jingwen Qiu, Yuan Chen, Qi Zhang","doi":"10.1007/s00253-025-13534-x","DOIUrl":"10.1007/s00253-025-13534-x","url":null,"abstract":"<p><p>Red yeasts demonstrate considerable potential in industrial and biotechnological applications, particularly in the biosynthesis of carotenoids and lipids, which are valuable secondary metabolites with a wide range of applications. In the oleaginous red yeast Rhodosporidium kratochvilovae YM25235, the acetyl-CoA synthetases RkACS1 and RkACS2 play critical roles in converting acetate into acetyl-CoA, a key precursor for the synthesis of various metabolites, including carotenoids and lipids. This study explores the physiological functions and metabolic regulation of RkACS1 and RkACS2, revealing distinct roles for these isoenzymes in metabolic processes. RkACS1 is essential for utilizing non-fermentable carbon sources such as acetate, ethanol, and glycerol, exhibiting high affinity for acetate and being activated by acetate while inhibited by glucose. Additionally, RkACS1 is involved in carotenoid biosynthesis. In contrast, RkACS2, while not specific to particular carbon sources, is primarily involved in lipid and fatty acid synthesis. It also influences gene expression through histone acetylation in the nucleus. Notably, these two isoenzymes exhibit functional redundancy and mutual regulation. These findings provide valuable insights into the metabolic regulation of acetyl-CoA synthesis, offering a foundation for engineering strategies aimed at optimizing secondary metabolite production in oleaginous red yeasts. KEY POINTS: • RkACS1 is related to carotenoid biosynthesis and essential for non-fermentable carbon sources • RkACS2 supports lipid and fatty acid biosynthesis and regulates histone acetylation in the nucleus • Functional redundancy and mutual regulation exist between RkACS1 and RkACS2 isoenzymes.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"140"},"PeriodicalIF":3.9,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of antibodies against severe fever with thrombocytopenia syndrome virus nucleoprotein for diagnosis.","authors":"Eun-Mee Park, Seheon Kim, Seoyoen Lim, Parastou Rahimizadeh, Hyeyoon Jeon, Hyung Jin Lim, Sungjin Lee, Yong Bhum Song","doi":"10.1007/s00253-025-13530-1","DOIUrl":"10.1007/s00253-025-13530-1","url":null,"abstract":"<p><p>Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral infectious disease caused by a novel Bandavirus in the family Phenuiviridae. The SFTS virus (SFTSV) is transmitted to various hosts, including humans, through tick bites, leading to high fever, thrombocytopenia, and leukopenia, with a high case fatality rate (up to 30%) due to multiple organ dysfunction. Therefore, early diagnosis is crucial for effective treatment and preventing disease transmission. In this study, we aimed to develop and characterize monoclonal antibodies targeting the SFTSV nucleocapsid protein (NP). We generated recombinant NP to screen antibodies against SFTSV. Using phage display technology, we identified candidate single-chain variable fragment (scFv) sequences capable of detecting SFTSV NP. Five human IgG antibodies and six chimeric mouse antibodies exhibited strong binding ability to the recombinant NP. Furthermore, their specificity and selectivity were evaluated against NPs from different subtypes and other viral species. A sandwich enzyme-linked immunosorbent assay (ELISA) was performed to determine optimal antibody pairings for SFTSV detection. The mP01A05/hP01C09, mP01A05/hP01B10, and mP02E04/hP01A05 antibody pairs demonstrated high efficacy in diagnosing SFTSV infections. These findings provide valuable antibody resources and establish an effective platform for the diagnosis of SFTS. KEY POINTS: • Monoclonal antibodies targeting SFTSV NP were developed using phage display technology. • Candidate antibodies showed strong binding ability and high specificity to SFTSV NP. • Optimized antibody pairs enabled effective SFTSV detection via sandwich ELISA.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"139"},"PeriodicalIF":3.9,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MFα signal sequence in yeast-based protein secretion: challenges and innovations'.","authors":"Magdalena Merkaš, Nina Grujicic, Martina Geier, Anton Glieder, Anita Emmerstorfer-Augustin","doi":"10.1007/s00253-025-13532-z","DOIUrl":"10.1007/s00253-025-13532-z","url":null,"abstract":"<p><p>Protein secretion in yeast is a complex, multistep process heavily reliant on signal sequences to guide recombinant proteins through the secretory pathway. Among these, the mating factor alpha (MFα) signal sequence from Saccharomyces cerevisiae has emerged as a powerful tool for enhancing the extracellular production of heterologous proteins. This review provides a comprehensive overview of the MFα signal sequence, tracing its historical development and role in advancing our understanding of protein secretion mechanisms, including co- and post-translational secretory pathways. We highlight key studies focused on optimizing the MFα signal sequence for improved secretion efficiency, leading to the development of several highly effective variants. These optimized sequences have significantly increased recombinant protein yield and quality, with notable implications for both research and industrial applications. Additionally, we explore the challenges of MFα-based secretion, including issues of missorting, incorrect processing, and aggregation in the endoplasmic reticulum (ER). We discuss emerging strategies to overcome these bottlenecks, such as fusion with alternative signal sequences and strain engineering. Finally, the review highlights current efforts to develop more robust signal peptides, and underscores the importance of continued innovation in protein secretion systems to meet the growing demand for high-quality recombinant proteins in biotechnological and therapeutic applications. KEY POINTS: • MFα remains the top choice for recombinant protein secretion in yeast • Challenges in secretion: ER aggregation, missorting, and processing errors • Mutated and hybrid signal peptides offer promising solutions.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"138"},"PeriodicalIF":3.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth and gene expression of Pseudomonas nitroreducens TX1 on octylphenol polyethoxylate surfactants.","authors":"Lam Dinh Bui, Po-Chun Tsai, Tuan Ngoc Nguyen, Chih-Chen Chen, Shir-Ly Huang","doi":"10.1007/s00253-025-13463-9","DOIUrl":"10.1007/s00253-025-13463-9","url":null,"abstract":"<p><p>Pseudomonas nitroreducens ATCC PTA-6168 (also named TX1) efficiently grows on non-ionic surfactants as the sole source of carbon and energy aerobically. The global gene expression and bacterial stress responses during degradation remain further investigated. This study compared the growth, viability, and transcriptomic profiles of strain TX1 during the log phase when grown on minimal salts basal medium supplemented with 0.5% octylphenol polyethoxylates (OPEO_n, commercial name Triton^® X-100) vs. in 0.5% succinate. Differentially expressed genes were identified, with 219 upregulated and 22 downregulated. Gene ontology analysis revealed upregulation of oxidoreductase activity (53%), electrotransfer activity (11%), heme/FAD/cofactor binding (11/19/27%), and membrane (42%)-related functions. In ethanol oxidation, adh18 and adh19 (for two pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases), aldh1 and aldh2 (for two aldehyde dehydrogenases), c₅₅₀ (for cytochrome c₅₅₀), and pqqABCDEF (for PQQ biosynthesis proteins) and aceA (for isocitrate lyase) and aceB (for malate synthase) acting in the glyoxylate cycle were upregulated. In addition to the above genes, the expression level of the representative genes from fatty acid degradation and chemotaxis was verified by RT-qPCR. The global gene expression of P. nitroreducens TX1 provides candidate genes involved in the bacteria grown on nonionic surfactants. KEY POINTS: • Growth characteristics of Pseudomonas nitroreducens ATCC PTA-6168 on surfactants • Differentially expressed genes related to growth on surfactants were identified • The ethanol oxidation system was upregulated when grown on surfactants.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"137"},"PeriodicalIF":3.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drosophila attraction, colonization, contagion, and mortality by Pseudomonas spp. and toxicity of their biosurfactants.","authors":"Argyro Tsipa, Maria Pettemereidi, Constantina K Varnava, Izel Ungor, Eftychia Fragkou, Yiorgos Apidianakis","doi":"10.1007/s00253-025-13518-x","DOIUrl":"10.1007/s00253-025-13518-x","url":null,"abstract":"<p><p>Oil bioremediation may be achievable via Pseudomonas spp. leading to low-cost biosurfactant (BSF) production, but the environmental impact is unclear. Here, we studied P. aeruginosa PA14 and PAO1; P. putida mt-2 and F1; and P. citronellolis 620C, P3B5, and SJTE-3, for their ability to degrade oily wastewater (OW), produce BSFs, and impact the model insect, Drosophila melanogaster. Biodegradation was > 86% by day 1 and > 93% by day 7, while BSF production was > 200 mg/L by day 1 and > 400 mg/L by day 7 for all strains. P. aeruginosa PAO1 and PA14 produce rhamnolipids and glycolipopeptides, respectively. P. putida mt-2 and F1 formed glycolipopeptides and glycolipids, respectively. P. citronellolis P3B5 and SJTE-3 yielded glycolipids, whereas 620C produced lipopeptides. Strikingly, Drosophila was mostly attracted to food contaminated with any of the P. aeruginosa strains or P. putida mt-2, which were the most virulent. To the contrary, Drosophila was repelled from food containing the low in virulence P. putida F1 or any of the P. citronellolis strains. All strains exhibited high ability to colonize Drosophila and disperse from fly to fly, but the colonization and contagion extend by P. aeruginosa strains were slightly higher. Moreover, the virulence of Pseudomonas spp. aligned with the toxicity of their BSFs. BSFs produced by P. aeruginosa were the most toxic, followed by P. putida and P. citronellolis, indicating a correlation between BSF toxicity and microbial origin. We concluded that P. citronellolis strains and their BSFs are relatively innocuous to the fly populations, yet highly potent in biodegrading OW. KEY POINTS: • >93% biodegradation of oily wastewater by all Pseudomonas spp. strains by day 7 • The virulence of Pseudomonas spp. aligns with the toxicity of their BSFs • P. citronellolis strains and their BSFs are more innocuous to Drosophila than those of P. putida and P. aeruginosa.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"135"},"PeriodicalIF":3.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular peptide production in Escherichia coli by inducible downregulation of lipoprotein Lpp via MicL sRNA.","authors":"Martin Gibisch, Pawel Gorecki, Christopher Tauer, Esther Egger, Matthias Müller, Bernd Albrecht, Rainer Hahn, Gerald Striedner, Monika Cserjan-Puschmann","doi":"10.1007/s00253-025-13524-z","DOIUrl":"10.1007/s00253-025-13524-z","url":null,"abstract":"<p><p>Despite its many benefits, Escherichia coli only poorly secretes recombinant proteins and peptides into the medium. This complicates downstream processing and notably contributes to the production costs of biopharmaceuticals. The permeability of production strains can be increased by deletion of the lpp gene, coding for Braun's lipoprotein Lpp. Consequently, the outer membrane (OM) is destabilized, and periplasmic recombinant proteins/peptides can leak out of the cell into the cultivation medium. However, we observed poor process performance during C-limited fed-batch cultivations in bioreactors when production strains with lpp knockout were cultivated. In this study, we developed an inducible system for in-process Lpp downregulation (knockdown) in E. coli with the goal to facilitate the release of the periplasmic recombinant fusion peptide CASPON-SST into the cultivation medium. By plasmid-based overexpression of MicL sRNA, we were able to efficiently inhibit Lpp synthesis and increase the OM permeability of our production strains. With this approach, we were able to achieve the secretion of 80-100% of all peptide and increased production capacities. The system was further optimized by utilizing different promoter systems to induce peptide and MicL expression separately in order to coordinate them. We report here for the first time the extracellular production of a recombinant peptide by inducible downregulation of Lpp via MicL sRNA during C-limited fed-batch cultivations. By utilizing a flexible system for Lpp knockdown, potential drawbacks of lpp knockout can be counteracted, thus making our approach a valuable tool for the in-process adaptation of OM permeability in production hosts. KEY POINTS: • Lpp can be downregulated on mRNA level by MicL sRNA overexpressed during fed-batch. • Recombinant peptides can leak out of the periplasm when Lpp is downregulated. • Leakiness and outer membrane permeability must be distinguished.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"136"},"PeriodicalIF":3.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of recombinant BCG containing the fusion gene BFNA on EB virus-positive tumors.","authors":"Shuo Huang, Shuyang Shao, Liding Fan, Junying Wang, Furen Meng, Siyuan Guan, Changhao Wang, Ang Liu, Yuanhui Wang, Qingjie Xue","doi":"10.1007/s00253-025-13528-9","DOIUrl":"10.1007/s00253-025-13528-9","url":null,"abstract":"<p><p>To obtain recombinant Bacillus Calmette-Guérin (rBCG) containing an Epstein-Barr virus (EBV) fusion gene that can inhibit EBV-positive cancer, we obtained BZLF1 and EBNA1 cDNA and overlapped these sequences to assemble the fusion gene BFNA. Then, BFNA was inserted into pMV261, and pMV-BFNA was transfected into BCG-competent cells. Western blotting was performed to detect the target fusion protein, specific antibodies were detected in the serum via enzyme-linked immunosorbent assays (ELISAs), and spleen cell-specific cytokines were detected via enzyme-linked immunospot (ELISPOT) assays. Cytotoxic T lymphocyte (CTL) activity, tumor weight, tumor formation time, and mouse survival were determined in EBV-positive tumor cell (NPRC18) cancer models, and flow cytometry was performed to analyze the quantities of CD8⁺ and CD4⁺ T cells in C57BL/6 J mice. Single-factor analysis of variance was performed with SPSS 19.0 to evaluate rBCG inhibition. The molecular weight of the fusion protein was approximately 55.5 kDa. The titer of the antibody in the rBCG group was highly significant (P ≤ 0.01), which delayed tumorigenesis, and the specific killing ability of rBCG targeting the recombinant target protein was increased. Compared with BCG-EBNA1 and BCG-BZLF1, the rBCG with the BFNA fusion gene presented a greater effect on tumors. Flow cytometric analysis revealed that the numbers of CD4⁺ T and CD8⁺ T cells in the blood of the rBCG group were significantly greater than those in the blood of the control group (P < 0.01). The mice injected with rBCG had greater lymphocyte infiltration in the tumor area. Thus, rBCG exerts a notable immune effect in mice and an inhibitory effect on EBV-positive tumor cell cancer models. KEY POINTS: • rBCG with the BFNA fusion gene induced strong immune responses and delayed EBV tumor growth. • rBCG promoted CD4+ and CD8+ T cell infiltration in EBV-positive tumor models. • The BFNA fusion protein effectively increased CTL activity and prevented tumor progression.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"134"},"PeriodicalIF":3.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of the thermophilic P450 CYP116B305 identified using metagenomics-derived sequence data from an Australian hot spring.","authors":"Simran Kundral, Peter D Giang, Leah R Grundon, Jenna M Supper, Sunil K Khare, Paul V Bernhardt, Paul Evans, Stephen G Bell, James J De Voss","doi":"10.1007/s00253-025-13521-2","DOIUrl":"10.1007/s00253-025-13521-2","url":null,"abstract":"<p><p>Cytochrome P450 enzymes (P450s) have gained significant attention due to their remarkable ability to oxidise unactivated C-H bonds with high regio- and stereoselectivity. Their industrial utility is often limited by challenges such as low stability, poor expression, and dependence on elusive redox partners. These issues have driven the search for more robust P450s, especially those that are inherently stable under extreme conditions typical of industrial processes. \"Self-sufficient P450s\" in which the P450 heme domain is naturally fused to redox domains in a single polypeptide chain eliminates the need to identify and separately express required redox partners. Furthermore, P450s from thermophilic organisms are more temperature tolerant with fewer stability issues. This study presents a self-sufficient P450, CYP116B305, identified from metagenomically assembled genomes from Innot Hot Springs (71 °C), located in North Queensland, Australia. CYP116B305 was heterologously expressed in Escherichia coli and purified using standard protocols. Investigation of the thermal stability of CYP116B305 revealed a robust heme domain with a ¹⁵T₅₀ value of 56.9 ± 0.1 °C, while the reductase domain exhibited slightly lower stability, with a ¹⁵T₅₀ value of 52.5 ± 0.5 °C. Further characterisation revealed that CYP116B305 efficiently bound to and hydroxylated 2-hydroxyphenylacetic acid (2-HPA) at the C-5 position, yielding homogentisic acid. The catalytic parameters, including the coupling efficiency and rate of electron transfer from the NADPH cofactor to the P450 heme, were shown to improve at an elevated temperature (45 °C) compared to 25 °C. The combination of the self-sufficiency and improved stability makes CYP116B305 a promising platform for biotechnological applications and biocatalyst engineering. KEY POINTS: • Hot spring metagenomics reveals thermostable P450s of biocatalytic value. • CYP116B305 shows enhanced catalytic activity at elevated temperature (45 °C). • CYP116B305 is a promising platform enzyme for diverse biotechnological use.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"133"},"PeriodicalIF":3.9,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering of Corynebacterium glutamicum for the synthesis of aromatic compounds.","authors":"Jan Marienhagen","doi":"10.1007/s00253-025-13520-3","DOIUrl":"10.1007/s00253-025-13520-3","url":null,"abstract":"<p><p>A significant proportion of industrially important small molecules are aromatic, and the majority of these compounds are produced chemically, relying heavily on fossil resources. In bacteria and plants, the shikimate pathway and related biosynthetic routes serve as the primary sources of aromatic compounds. Microbial cell factories, which are poised to play a central role in the emerging bio-based economy, provide a sustainable alternative for producing commercially valuable aromatics from renewable resources. Corynebacterium glutamicum, already established as an industrial workhorse for the large-scale production of various amino acids, can be engineered to overproduce aromatic compounds derived from the shikimate pathway. Furthermore, the functional integration of heterologous or synthetic pathways enables access to high-value natural products, such as plant polyphenols and other polyketides. This review highlights recent advancements in the metabolic engineering of C. glutamicum for the sustainable production of these aromatic compounds. KEY POINTS: • C. glutamicum's high tolerance to aromatic compounds is key to aromatics production. • Detailed physiological insights enable access to shikimate pathway-derived products. • Diverse plant (poly)phenols and other aromatic polyketides can be produced.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"132"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}