Estefany Chavarria-Quicaño, Miguel A. Hernández-Oñate, Jorge A. Rodríguez-González, Francisco De la Torre-González, Ali Asaff-Torres
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引用次数: 0
Abstract
Lipopeptides (LPs) are secondary metabolites predominantly produced by Bacillus species and have significant industrial applications. The synthesis of these compounds is regulated by quorum sensing systems, which are activated in response to abiotic stress. This study examined the impact of heat and shear stresses on the growth, LPs production, and phenotype formation of the Bacillus paralicheniformis TB197 strain in controlled submerged cultures. A differential expression analysis was performed on genes associated with phenotypes, including biofilms (tasA), motility (hag), sporulation (sspB), and the production of lipopeptides (fenB and lichA). The findings demonstrated that, under conditions of 30 °C, an increase in agitation levels from 300 to 600 rpm resulted in a 1.5-fold enhancement in cell concentration and a 2.8-fold increase in LPs production. At 40 °C, these values were 2.80- and 2.06-fold, respectively. Furthermore, fenB and lichA expression levels increased by 17- and 36-fold, respectively, suggesting that the combined stress of 40 °C and 600 rpm promotes the development of LP-producing phenotypes. However, at 45 °C, although cell concentration showed no difference compared to 40 °C, LPs production decreased 2.49-fold, with reductions in lichA (sixfold) and fenB (65-fold) expression. Under these conditions, a predominance of sporulating and biofilm-forming phenotypes was observed, supported by increased expression of tasA and sspB genes. These findings show that thermal and mechanical stresses differentially modulate the expression of phenotypes and the production of LPs in B. paralicheniformis TB197.
期刊介绍：
Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.
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