Applied Microbiology and Biotechnology最新文献

{"title":"Interkingdom communication 2.0: pheromone-based control between Bacillus subtilis and Saccharomyces cerevisiae","authors":"Julia Döring,&nbsp;Tomislav Vološen,&nbsp;Kai Ostermann,&nbsp;Diana Wolf","doi":"10.1007/s00253-026-14069-5","DOIUrl":"10.1007/s00253-026-14069-5","url":null,"abstract":"<p>Microbial communities found in nature are complex and thrive in harmony, consisting of organisms derived from several kingdoms. Hence, they need to be able to communicate by quorum-sensing molecules to co-exist in close proximity. Being able to engineer artificial interkingdom communication for biotechnological approaches, such as degradation of complex biomaterials in one fermenter using controlled signaling between microorganisms, would be beneficial. Therefore, we wanted to establish a proof of principle by enabling the Gram-positive bacterium <i>Bacillus subtilis</i> to communicate in a controlled manner with the eukaryotic yeast <i>Saccharomyces cerevisiae</i> by exploiting the yeast’s pheromone system. We genetically modified <i>B. subtilis</i> to heterologously produce the α-pheromone after bacitracin induction, which in turn would activate the production of the green fluorescent protein mNeongreen upon pheromone recognition by <i>S. cerevisiae</i>. Co-cultivation in modified growth medium and induction of pheromone expression was successful and repeatable. Yeast activation and production of mNeongreen was visible not only by analysis but also by epifluorescence microscopy of the co-culture. This study successfully demonstrated controlled interkingdom signaling between microorganisms by leveraging their native communication systems.</p><p>• <i>Heterologous production and secretion of small peptides in Bacillus subtilis.</i></p><p>• <i>Interkingdom communication between bacteria and yeast.</i></p><p>• <i>Signaling by artificially produced pheromones.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14069-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149189570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A versatile Bacillus esterase reveals a carboxylesterase blueprint for PET intermediate degradation","authors":"Hadjira Bounabi,&nbsp;Bilal Yahiaoui,&nbsp;Meriem Gasmi,&nbsp;Meroua Safa Mechouche,&nbsp;Nico Bäse,&nbsp;Marno Gurschke,&nbsp;Rebecka Molitor,&nbsp;Pablo Pérez-García,&nbsp;Wolfgang R. Streit","doi":"10.1007/s00253-026-14056-w","DOIUrl":"10.1007/s00253-026-14056-w","url":null,"abstract":"<p>The biodegradation of polyethylene terephthalate (PET), and its derived intermediate breakdown products, represents a core focus of multidisciplinary research. While <i>Ideonella sakaiensis</i> PETase (IsPETase) and leaf-compost cutinase (LCC) efficiently depolymerize PET, many other PET-active hydrolases display stronger activity toward soluble PET intermediates such as bis- and mono(2-hydroxyethyl) terephthalate (BHET and MHET). Here, we describe a versatile carboxylesterase derived from <i>Bacillus halotolerans</i> (BhEstB). BhEstB is structurally related to BsEstB from <i>B. subtilis</i> but divergent from canonical PETases and most characterized MHETases, including IsMHETase, while clustering more closely with carboxylesterases such as TfCa. BhEstB displayed broad esterase activity toward <i>p</i>NP-esters, with maximum activity at pH 8 and 20–30 °C. Although it showed modest overall activity on the bulk polymer, BhEstB significantly outperformed the engineered variant LCC^ICCG and the recently published archaeal feruloyl esterase PET46 in turning over BHET and MHET. BhEstB exhibits remarkably high activity toward BHET and MHET, achieving complete conversion of 50 mM BHET within 1 h at 30 °C (kcat = 15.57 ± 1.25 s⁻¹, Km = 6.54 ± 1.01 mM, corresponding to a catalytic efficiency of 2.38 × 10³ M⁻¹ s⁻¹) and subsequent efficient turnover of 39.8 mM MHET (77% (± 0.7%)) into terephthalic acid (TPA) within 24 h. These results identify BhEstB as one of the most efficient BHET-hydrolyzing enzymes characterized to date. Collectively, these findings establish BhEstB as a versatile BHET/MHET active <i>Bacillus</i> carboxylesterase with modest activity toward commercial semi-crystalline PET powder, contributing to the expanding diversity of PET-intermediate-degrading enzymes.</p><p>• <i>BhEstB exhibits structural divergence from canonical PET esterases.</i></p><p>• <i>BhEstB efficiently degrades significant amounts of BHET and MHET at 30 °C.</i></p><p>• <i>BhEstB demonstrates moderate activity on PET powder at elevated temperatures.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14056-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alluvial filtration as a scalable and safe single-use technology for the harvest of viral vectors","authors":"Johanna Wiesner,&nbsp;Amelie Bauer,&nbsp;Detlef Eisenkraetzer","doi":"10.1007/s00253-026-14035-1","DOIUrl":"10.1007/s00253-026-14035-1","url":null,"abstract":"<p>Recent advances in gene therapy have increased the demand for large-scale manufacturing processes. The production of recombinant adeno-associated viral (rAAV) vectors, the predominant vectors used for gene delivery, requires efficient scalable processes for production and purification which have to comply with high safety standards. To enable operation in a fully closed system, the exclusive use of single-use materials and equipment is indispensable. The present study reports on the use of alluvial filtration for the clarification of lysed rAAV vector production culture from various production scales. In small-scale experiments (1–3 L feed), the in-house developed method was superior to the use of a synthetic depth filter regarding clarification performance for capacity, turbidity reduction, product recovery, and impurity reduction. Scale-up calculations for 500 L harvest revealed that alluvial filtration could provide the required filter area and capacity within a fully closed system, whereas the use of the depth filter could not. Real-life scale-up data for processing 10 L, 100 L, and 500 L with different rAAV vector variants confirmed scalability of the alluvial filtration method and yielded excellent results regarding its clarification performance. Moreover, the full filter capacity and limits of the system were not reached, indicating the robustness of the process to varying feedstock compositions. The safety of the established alluvial filtration train was supported by implementing enhanced monitoring and control to mitigate the risk of failure and leakages. Overall, the harvest of rAAV vectors by alluvial filtration proved to be superior to established depth filters within a biopharmaceutical production process.</p><p>• <i>Large-scale viral vector production (&gt; 100 L) to meet increasing gene therapy demand</i></p><p>• <i>Alluvial filtration outperforms depth filters in efficient harvest clarification</i></p><p>• <i>Alluvial filtration offers capacity for closed-system scale-up to 500 L production</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What we (still don’t) know about wood-degrading fungi: an overview of threats and utilization potentials","authors":"Elisabeth Tamayo,&nbsp;Felix Bachmann,&nbsp;Tian Cheng,&nbsp;Tim K. Felle,&nbsp;Marcello Nussbaumer,&nbsp;J. Philipp Benz","doi":"10.1007/s00253-026-14043-1","DOIUrl":"10.1007/s00253-026-14043-1","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13620020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene Oxide and Methylglyoxal: a combined strategy against chronic wound pathogens","authors":"Silvia Di Lodovico,&nbsp;Antonella Fontana,&nbsp;Paola Di Fermo,&nbsp;Firas Diban,&nbsp;Emanuela Di Campli,&nbsp;Serena Pilato,&nbsp;Simonetta D’Ercole,&nbsp;Luigina Cellini,&nbsp;Mara Di Giulio","doi":"10.1007/s00253-026-14050-2","DOIUrl":"10.1007/s00253-026-14050-2","url":null,"abstract":"<div><p>Chronic wound infections are difficult to treat due to the polymicrobial biofilms that delay wound healing increasing antimicrobial tolerance and limiting patient compliance. To address this challenge, we developed a non-antibiotic combination of Graphene Oxide (GO) and Methylglyoxal (MGO) (patent N. 102022000024408-Composizione per il trattamento delle infezioni a carico delle lesioni cutanee) against clinically relevant chronic wound pathogens. For the tests, clinical antimicrobial resistant <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> were used. MGO MIC was determined and the best GO + MGO combination, evaluated by using checkboard test, was tested in terms of: i) fluid membrane changes; ii) <i>S. aureus</i> and <i>P. aeruginosa</i> CFUs/mg reduction in Lubbock Chronic Wound Biofilm-LCWB, a recognized polymicrobial in vitro chronic wound biofilm model; iii) <i>P. aeruginosa</i> motility. MGO MIC values ranged from 32 to 128 mg/l. Notably, synergistic interactions between GO and MGO were observed exclusively against <i>S. aureus</i>, whereas the combinations showed additive effects against <i>P. aeruginosa</i>. The best GO + MGO (6.25 + 64 mg/l) combination increased bacterial membrane fluidity, inhibited informing and mature LCWBs by 60–80% in terms of CFU/mg and <i>P. aeruginosa</i> twitching motility. GO + MGO combination exhibited an additive antimicrobial/antibiofilm activity with a multi-target action. Overall, the GO + MGO combination, at recognized non-toxic concentrations, is a valid and innovative non-antibiotic solution for wound management affecting the polymicrobial chronic wound biofilms and <i>P. aeruginosa</i> motility.</p></div>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14050-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation-based oxalic acid production aimed at sustainable critical metal recovery from electronic waste","authors":"Aylin Nur Erkmen,&nbsp;Roland Ulber,&nbsp;Thomas Jüstel,&nbsp;Mirjam Altendorfner","doi":"10.1007/s00253-026-14040-4","DOIUrl":"10.1007/s00253-026-14040-4","url":null,"abstract":"<p>This study systematically investigates the biogenic production of oxalic acid (OA, H₂C₂O₄) via submerged fermentation to establish a sustainable reagent for critical metal recovery applications. Preliminary shake-flask experiments evaluated <i>Aspergillus niger</i> strains (ATCC 1015 and CECT 2807), optimal carbon sources, and the influence of pH adjustment. Despite inherent biological variance across replicates, <i>A. niger</i> ATCC 1015 demonstrated a superior production trajectory, yielding 71.0 ± 27.7 mM OA in 5 days. Intermittent pH adjustment above 4.0 significantly enhanced secretion to 90.4 ± 5.8 mM, while glucose was identified as the optimal carbon source (Y_P/S <span>(approx )</span> 0.4 g/g). Building upon these preliminary findings, process intensification was conducted in a 10 L stirred-tank bioreactor. Transitioning to a fed-batch strategy with pulsed feeding and continuous pH control effectively contributed to substantially higher OA titers, achieving a peak OA titer of 260.1 ± 4.8 mM over 14 days. Co-production of gluconic acid and acidogenesis inhibition posed a challenge in attaining higher product yields. Collectively, these findings establish a robust, scalable bioprocess for sustainable OA generation, directly supporting its emerging application as a highly selective leaching agent for critical metal recovery from electronic waste streams.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14040-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149029724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Efficient biotechnological production of N-(Hydroxycinnamoyl) tyramines by utilizing plant-derived enzymes in a bench-scale bioreactor with in situ product precipitation","authors":"Israa Abuobida Mohamed,&nbsp;Qais Al-Khawlani,&nbsp;Raika Milde,&nbsp;Niels Valentin Heise,&nbsp;Martin Dippe,&nbsp;Mohamed Nagia,&nbsp;Ludger A. Wessjohann,&nbsp;Markus Pietzsch,&nbsp;Bodo Moritz","doi":"10.1007/s00253-026-14044-0","DOIUrl":"10.1007/s00253-026-14044-0","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14044-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disulfiram reverses fluconazole-resistance mediated by Cdr1 in Candida albicans and interferes with biofilm formation","authors":"Ahmed Rezk,&nbsp;Heba S. Said,&nbsp;Sherin M. Elfeky,&nbsp;Mohammed El-Mowafy,&nbsp;Ramadan Hassan","doi":"10.1007/s00253-026-13977-w","DOIUrl":"10.1007/s00253-026-13977-w","url":null,"abstract":"<p>The incidence of <i>Candida albicans</i> infections has risen markedly, particularly among immunocompromised individuals and cancer patients undergoing chemotherapy. Fluconazole is commonly used as a first-line treatment for <i>C. albicans</i> infections; however, increasing clinical resistance poses a significant therapeutic challenge. As a result, developing new strategies to enhance the management of <i>C. albicans</i> infections has become critical. This study aims to assess the prevalence of azole’s resistance among clinical <i>C. albicans</i> isolates in Egypt and to evaluate disulfiram/fluconazole combination against fluconazole resistant isolates. A total of 64 <i>C. albicans</i> clinical isolates were tested for fluconazole susceptibility using disc diffusion method and broth microdilution method (alone and in combination with disulfiram). The combination of fluconazole and disulfiram was further assessed using checkerboard method. The expression levels of resistance determinants were analyzed using RT-PCR. Molecular docking was used for further analysis. Sixteen isolates (25%) were resistant to fluconazole. Combination with subinhibitory concentration of disulfiram reduced the minimum inhibitory concentration (MIC) of fluconazole in resistant isolates (2–128 fold). Checkerboard assay revealed that fluconazole/disulfiram combination was mostly synergistic. In fluconazole-resistant isolates, <i>Cdr1</i> was overexpressed, whereas disulfiram (at subinhibitory concentration) has triggered its downregulation. Docking studies showed that disulfiram may compete with ATP for binding and may disrupt fluconazole's interaction with <i>Cdr1</i>. Additionally, disulfiram exhibited a potent anti-biofilm activity against the tested <i>C. albicans</i> isolates. Fluconazole/disulfiram combination appears to be a promising approach for overcoming fluconazole resistance in <i>C. albicans</i>. Further studies are needed to validate this finding and explore its clinical potential.</p><p>•<i> Fluconazole/disulfiram combination is a promising approach to overcome fluconazole resistance in C. albicans</i></p><p>• <i>Disulfiram triggered downregulation of Cdr1 expression in fluconazole-resistant C. albicans.</i></p><p>• <i>Disulfiram competes with ATP for Cdr1 binding, disrupting fluconazole-Cdr1 interaction.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13977-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148954622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species","authors":"Maximilian Hilkmann,&nbsp;Norma Welsch,&nbsp;Max Fabian Felle,&nbsp;Thomas Schweder,&nbsp;Mathis Appelbaum","doi":"10.1007/s00253-026-14030-6","DOIUrl":"10.1007/s00253-026-14030-6","url":null,"abstract":"<p>Advances in molecular biology tools are essential for streamlining and accelerating genetic engineering of cells across industrial and academic applications. While CRISPR-Cas improves genome editing efficiency, current systems have limitations and are often host specific, which restricts their versatility. This study describes a versatile CRISPR-Cas9 system for genome editing in industrially relevant <i>Bacillus</i> species. By adapting the well-established pJOE8999 vector-based CRISPR-Cas9 genome editing system, we constructed an inducer-independent, broad-host-range genome editing system. It maintains the benefits of low toxicity to the target cell and the cloning host as well as the ease to use of a single-plasmid CRISPR-Cas9 system. We utilized the constitutive Sigma70-type promoter from the conserved <i>veg</i> gene of <i>Bacillus</i>, to develop and test the suitability of promoter variants of different strengths for Cas9 expression. Successful gene deletions in three different <i>Bacillus</i> species demonstrated the versatility of the modified system for this industrially important genus. This was further confirmed by the integration of a reporter gene fusion and the introduction of a single point mutation in the genome of <i>Bacillus licheniformis</i>. This one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time.</p><p>• <i>Editing and screening of promoter variants for balanced Cas9 expression in Bacillus.</i></p><p>• <i>Development of a versatile inducer-independent, single-plasmid CRISPR-Cas-based system.</i></p><p>• <i>Verification of the modified CRISPR-based system for genome editing in different Bacilli.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14030-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic consortia towards photosynthetically derived acetate for heterotrophic production of 1-butanol","authors":"Stamatina Roussou,&nbsp;Peter Lindblad","doi":"10.1007/s00253-026-14029-z","DOIUrl":"10.1007/s00253-026-14029-z","url":null,"abstract":"<p>Synthetic consortia is an emerging field in biotechnology, offering advantages such as division of labor between the individual members and reduced risk of contamination. Consortia combining phototrophic and heterotrophic bacteria are attractive, as phototrophs can utilize CO₂ and light energy to sustain growth. Several studies have explored such systems where sucrose is most commonly used as the provided carbon source. An alternative is acetate, a smaller two-carbon molecule produced as a by-product by many microorganisms, including the model cyanobacterium <i>Synechocystis</i> PCC 6803 (thereafter <i>Synechocystis</i>). Although acetate production during phototrophic growth is typically low, previous studies have demonstrated that metabolic engineering—specifically the introduction of phosphoketolase (PK) and overexpression of phosphotransacetylase (Pta)—enabled the development of a high-producing strain (WT_PKPa_RBS_BsPta_Δacs) capable of secreting significant levels of acetate into the medium (Roussou and Lindblad 2026). In this study, <i>Escherichia coli</i> and <i>Pseudomonas taiwanensis</i> were engineered to produce 1-butanol, an industrially relevant bulk chemical, and cultivated using acetate as the sole carbon source. These strains were then individually co-cultivated with the previously engineered <i>Synechocystis</i> strain, WT_PKPa_RBS_BsPta_Δacs, forming two distinct consortia that were maintained for 42 days. Growth dynamics were successfully monitored, and acetate concentrations in the consortia were lower than in a corresponding axenic <i>Synechocystis</i> culture. At the same time, 1-butanol production was detected in the two co-cultures, demonstrating the feasibility of coupling photosynthetically derived acetate to heterotrophic production of a value-added bulk chemical.</p><p>\u0000<b>•</b>\u0000<i>Photosynthetic/heterotrophic synthetic consortia for 1-butanol production</i>.</p><p><b>• </b><i>Engineered Synechocystis PCC 6803 cells produce acetate from CO</i>_<i>2</i>.</p><p><b>• </b><i>Modified Escherichia coli and Pseudomonas taiwanensis cells grow on acetate and produce 1-butanol</i>.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-14029-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}