Applied Microbiology and Biotechnology最新文献

{"title":"Transcriptome changes of fission yeast cells exposed to fumonisin B1 or co-cultured with Fusarium verticillioides","authors":"László Attila Papp,&nbsp;Lajos Acs-Szabo,&nbsp;Szilvia Kovács,&nbsp;Cintia Adácsi,&nbsp;Gyula Batta,&nbsp;Tünde Pusztahelyi,&nbsp;István Pócsi,&nbsp;Ida Miklós","doi":"10.1007/s00253-025-13601-3","DOIUrl":"10.1007/s00253-025-13601-3","url":null,"abstract":"<p><i>Fusarium verticillioides</i> poses a high food safety risk worldwide due to its mycotoxin production. Successful control of Fusaria may rely on promising biocontrol agents, including yeasts. Although the fission yeast <i>Schizosaccharomyces pombe</i> tolerated <i>Fusarium</i> mycotoxins well, including zearalenone, T2, deoxynivalenol, and fumonisins (FUMs), it did not significantly inhibit the growth of <i>F. verticillioides.</i> Meanwhile fumonisin B1 (FB1) supplementation did not decrease <i>S. pombe</i> cell density in submerged liquid cultures, the colony-forming capability of the yeast was reduced. RNA sequencing showed that <i>S. pombe</i> genes involved in cell adhesion and flocculation were downregulated after FB1 exposure. In addition, the expression of several hydrolase genes was also altered. In co-cultures with <i>F. verticillioides</i>, genes encoding oxidoreductases and hydrolases and those linked to purine nucleotide metabolisms were downregulated, while the expression of genes involved in membrane and transport processes was increased. The expression of several <i>F. verticillioides</i> genes also changed after co-cultivation. Oxidoreductase, transmembrane transport, and purine metabolism genes were upregulated under co-culturing; meanwhile, hydrolase genes, together with carbon metabolism and polysaccharide catabolism genes, were downregulated. Co-cultivation also decreased fumonisin production via the downregulation of genes <i>FUM19</i>, <i>FUM21</i>, and <i>FvATFA</i> encoding the fumonisin transporter, a local Zn(II)2Cys6-type transcriptional regulator and an important global regulator bZIP-type transcription factor, respectively. Although further experiments should clarify the mechanism of the fission yeast-elicited inhibition of fumonisin production, these results may pave the way for the development and implementation of novel, innovative approaches to control mycotoxin production by <i>F. verticillioides</i> in the feed and food chain.</p><p>• <i>0.5 ppm FB1 reduced the colony-forming ability of S. pombe and caused transcriptional changes.</i></p><p>• <i>Expression of transport and hydrolase genes changed in yeast during co-cultivation with mold.</i></p><p>• <i>Two FUM cluster genes and FvATFA were downregulated in Fusarium co-cultured with S. pombe.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13601-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing laboratory cultivation of wood-inhabiting fungi with emphasis on applied conservation","authors":"Joette Crosier,&nbsp;Lorin von Longo-Liebenstein,&nbsp;Mattias Edman,&nbsp;Sylwia Adamczyk,&nbsp;Leena Hamberg","doi":"10.1007/s00253-025-13603-1","DOIUrl":"10.1007/s00253-025-13603-1","url":null,"abstract":"<p>While fungi have been grown for centuries as food, cultivation knowledge rarely extends to rare fungi, usually confined to those with high biotechnology or food value. A more robust knowledge base on cultivating rare, challenging fungi may be critical for conservation, as many species face extinction. They should be cultivated for gene banks, reintroduction, and other conservation work. This study adapted mushroom growing techniques for seven threatened wood-inhabiting fungi, all red-listed in Finland and Sweden: <i>Antrodia crassa</i>, <i>Antrodia infirma</i>,<i> Amylocystis lapponica</i>,<i> Skeletocutis stellae</i>,<i> Perenniporia tenuis</i>,<i> Radulodon erikssonii</i>, and <i>Haploporus odorus</i>. We grew mycelium of these species (five strains each, two for <i>P. tenuis</i>) under various laboratory conditions. We tested wood dust supplementation (in agar), grain spawn substrate composition, gas exchange rates, two wood types (natural host and birch) on sawdust and dowel spawn, and temperature range (6.0–36.5 °C). We measured growth rate in all conditions and ergosterol (mycelial biomass indicator) in wood type and wood dust agar tests. We found wood dust–supplemented agar had an overall positive effect. Temperature effects varied by species, with some preferring relatively warm or cool temperatures, and some having a narrower growth range. Most species grew better on grain when vermiculite was added; gas exchange had no effect. Wood type had a variable effect, but birch was suitable in all cases, sometimes better than the natural host wood. Overall, our treatments had positive to neutral effects on mycelial growth of our fungal species.\u0000</p><p>• <i>Optimized laboratory cultivation methods can benefit fungal conservation and other applied mycology efforts.</i></p><p>• <i>Certain supplements for increasing media complexity or retaining substrate moisture lead to improved growth of challenging fungi.</i></p><p>• <i>Optimal conditions vary by species and strain, but general guidelines may apply more broadly, and natural habitat conditions can offer a starting point.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13603-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized biosynthesis of bioactive silver nanoparticle-kombucha cellulose nanocomposites for enhanced antimicrobial applications","authors":"M. Bassam Aboul-Nasr,&nbsp;Alaa A. Yasien,&nbsp;Sabah S. Mohamed,&nbsp;Marwa Obiedallah","doi":"10.1007/s00253-025-13585-0","DOIUrl":"10.1007/s00253-025-13585-0","url":null,"abstract":"<p>Sustainable biosynthesis of monodisperse, bioactive silver nanoparticles (AgNPs) integrated with nanofabricated kombucha SCOBY cellulose membrane (KC) was achieved using the endophytic fungus <i>A</i>. <i>fumigatiaffinis</i> PP235788.1 fungal filtrate. This study is the first to optimize AgNP synthesis via full-factorial design of experiments (DOEs) and response surface methodology (RSM), identifying ideal conditions (2 mmol L⁻¹ AgNO₃, pH 8 and 60 °C). Characterization revealed spherical AgNPs (14.50 ± 0.58 nm) and AgNPs@KC composites (17.88 ± 0.36 nm); SEM imaging demonstrates the successful fusion of AgNPs within KC’s fibrous membrane, creating a seamlessly integrated nanocomposite with enhanced functional architecture. The formed nanoparticles were composed of crystalline mettalic silver, as varified by XRD (detected planes at 111, 200, and 220), and were stabilized by amide groups, as identified by FTIR spectroscopy. The biosynthesized AgNPs demonstrated a dose-dependent antimicrobial activity with an increase in inhibition zones from 11.8 ± 2.1 mm at 10 µg/mL to 36.1 ± 0.4 mm at 100 µg/mL for <i>E</i>. <i>coli</i> and from 9.8 ± 2.9 mm to 34.33 ± 1.2 mm for <i>Bacillus subtilis</i>. Fungal pathogens displayed diminished sensitivity, with <i>Aspergillus niger</i> achieving maximum inhibition (25.7 ± 0.3 mm) at 100 µg/mL, whereas <i>Candida</i> spp. necessitated ≥ 60 µg/mL for observable inhibition zones. AgNPs@KC composites demonstrated broad-spectrum efficacy against 12 pathogens (ANOVA, <i>p</i> &lt; 0.001, <i>R</i>² &gt; 95%), with maximal activity against <i>E</i>. <i>coli</i>, <i>B</i>. <i>subtilis</i>, and <i>A</i>. <i>niger</i> (33.0 ± 0.2 mm). These findings highlight the integration of mycosynthesized AgNPs with kombucha SCOBY cellulose membranes, offering a novel and unique platform for antimicrobial approaches with exceptional purity and distinctive physiological characteristics.</p><p>• <i>AgNPs optimized via DOE/RSM.</i></p><p>• <i>AgNPs@KC shows potent antimicrobial activity.</i></p><p>• <i>Novel mycosynthesis of AgNPs with KC membranes.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12479647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, expression, and anti-cancer effects of the novel fusion protein rONC-T7-pHLIP","authors":"Ganggang Yang,&nbsp;Shaokang Wang,&nbsp;Yuhan Wang,&nbsp;Haigang Zheng,&nbsp;Meng Du,&nbsp;Ze Wang,&nbsp;Zhuo Chen,&nbsp;Cuifang Chang,&nbsp;Guoying Yu","doi":"10.1007/s00253-025-13578-z","DOIUrl":"10.1007/s00253-025-13578-z","url":null,"abstract":"<p>Onconase (ONC), a novel antitumor protein, exhibits significant cytotoxic effects on various tumor cells. Although recent advancements have been made in ONC-based molecularly targeted drugs, most are single-targeting proteins with limitations such as poor targeting precision and low efficiency. In this study, the fusion protein rONC-T7-pHLIP was constructed by linking ONC with the T7 peptide, which specifically recognizes the transferrin receptor, and the pHLIP peptide, which actively targets the acidic tumor microenvironment, using a flexible linker peptide (GGGGS)₃. The engineered strain <i>E. coli</i> BL21(DE3) / rONC-T7-pHLIP was used for single-factor analysis of IPTG concentration, induction time, and induction temperature, followed by orthogonal experimental design to optimize the expression conditions, resulting in a 10% increase in fusion protein expression. Cytotoxicity and flow cytometry apoptosis assays demonstrated that the purified fusion proteins—dual-targeting rONC-T7-pHLIP and single-targeting rONC-T7 and rONC-pHLIP—exhibited significantly higher antitumor activity against cancer cells compared to native ONC, with the dual-targeting variant showing superior efficacy over the single-targeting ones. Immunofluorescence assays confirmed that rONC-T7-pHLIP binds to cancer cells and exerts its activity in the cytoplasm. In conclusion, these findings suggest that the novel fusion protein rONC-T7-pHLIP has potential as a targeted therapeutic agent for cancer treatment.</p><p>• <i>The rONC-T7-pHLIP was expressed in the E. coli expression system with high yield.</i></p><p>• <i>The rONC-T7-pHLIP showed high stability and safety in vitro.</i></p><p>• <i>The rONC-T7-pHLIP significantly improved the antitumor activity of cancer cells.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13578-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina","authors":"Abhijit Dey,&nbsp;Debleena Roy,&nbsp;Vikas Moreshwar Mohture,&nbsp;Mimosa Ghorai,&nbsp;Md. Habibur Rahman,&nbsp;Uttpal Anand,&nbsp;Saikat Dewanjee,&nbsp; Radha,&nbsp;Manoj Kumar,&nbsp;Dorairaj Arvind Prasanth,&nbsp;Niraj Kumar Jha,&nbsp;Saurabh Kumar Jha,&nbsp;Mahipal S. Shekhawat,&nbsp;Devendra Kumar Pandey","doi":"10.1007/s00253-025-13609-9","DOIUrl":"10.1007/s00253-025-13609-9","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13609-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foot-and-mouth disease vaccine with insertion of a 24-amino acid VP1 G-H loop epitope of the Cathay virus provides broad antigenic coverage","authors":"Keqiang Zhang,&nbsp;Jingjing Zha,&nbsp;Pu Sun,&nbsp;Yifan Ouyang,&nbsp;Xingze Zhang,&nbsp;Xueqing Ma,&nbsp;Feng Liu,&nbsp;Dong Li,&nbsp;Huifang Bao,&nbsp;Yimei Cao,&nbsp;Xingwen Bai,&nbsp;Yuanfang Fu,&nbsp;Kun Li,&nbsp;Hong Yuan,&nbsp;Jing Zhang,&nbsp;Zhixun Zhao,&nbsp;Jian Wang,&nbsp;Qiang Zhang,&nbsp;Zaixin Liu,&nbsp;Zengjun Lu,&nbsp;Pinghua Li","doi":"10.1007/s00253-025-13545-8","DOIUrl":"10.1007/s00253-025-13545-8","url":null,"abstract":"<p>Vaccination with inactivated whole-virus vaccines remains the most effective measure for controlling foot-and-mouth disease virus (FMDV) transmission and disease outbreaks. However, the existing type O FMDV vaccines show suboptimal efficacy and antigenic mismatch to the circulating Cathay viruses in China, thus requiring the development of a new vaccine. The VP1 G-H loop is a hypervariable region and plays a pivotal role in the protective immunity induced by FMDV vaccines. Here, we engineered four recombinant FMDVs with insertions of a 20-amino acid (aa) or a 24-aa G-H loop epitope of a prevalent Cathay strain upstream or downstream of the RGD (Arg-Gly-Asp) motif. The recombinant viruses with insertions upstream of the RGD motif retained parental virus-like plaque morphology and replication kinetics and maintained genetic stability even after 20 serial passages. In contrast, the downstream insertion variants exhibited small plaque morphology, reduced growth capacity, and acquired 1 or 2 aa mutations in the capsid proteins by passage 20. The parental virus vaccine induced high titer protective mean neutralizing antibodies (&gt; 1:128) against viruses of the Mya98, PanAsia, and Ind-2001 lineages but failed to elicit protective mean neutralizing antibodies (&lt; 1:22) to the Cathay virus after 28 days vaccination (dpv) in pigs. In contrast, vaccines containing upstream insertions both exhibited protective immune response to viruses of four lineages. Especially, pigs vaccinated with vaccine containing a 24-aa insertion produced significantly higher mean neutralizing antibody against the Cathay virus (<i>p</i> &lt; 0.01), compared to those vaccinated with vaccine having a 20-aa insertion, indicating that the recombinant virus with 24-aa insertion has great potential as a vaccine candidate for serotype O FMD control. This study provides crucial insights for designing FMDV vaccines in the future.</p><p>• <i>This study firstly reported that FMDV can tolerate a 24-aa insertion in the VP1 G-H loop</i></p><p>• <i>The G-H loop insertions at different sites of FMDV VP1 have different impacts on viral replication capacity</i></p><p>• <i>Vaccines containing the G-H loop insertions can induce markedly high neutralizing antibodies to the Cathay virus</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13545-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria dynamics and its correlation with chemical composition changes in tobacco leaves during flue curing","authors":"Yunfei Sha,&nbsp;Mengqian Zhou,&nbsp;Demin Liang,&nbsp;Jie Yu,&nbsp;Yanjiu Bi,&nbsp;Qiansi Chen,&nbsp;Huina Zhou,&nbsp;Xianchao Shang,&nbsp;Da Wu","doi":"10.1007/s00253-025-13598-9","DOIUrl":"10.1007/s00253-025-13598-9","url":null,"abstract":"<div><p>Microorganisms play a vital role in tobacco growth and processing, yet their correlation with chemical composition during flue curing is understudied. This study examines the dynamic changes in chemical components and bacterial communities during flue curing of lower, middle, and upper leaves. Several key constituents showed distinct changes across leaf positions and curing stages. Xanthophyll decreased during the yellowing stage, while neutral fragrance substances increased after yellowing, especially in middle leaves. Sucrose and proline showed a predominant increasing trend throughout the entire curing process. Nicotine in lower leaves was lowest, and significantly increased by flue curing. Bacterial community analysis revealed higher alpha diversity in lower leaves, dominated by <i>Proteobacteria</i>, with <i>Pseudomonas</i> and <i>Acinetobacter</i> prevalent in lower and middle leaves, and <i>Aeromonas</i> in upper leaves. The flue-curing process, especially the yellowing stage, significantly altered bacterial community structure. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) analysis revealed stage- and leaf position-dependent variations in predicted microbial metabolic functions, with predominant involvement in amino acid, lipid, and carbohydrate metabolism pathways. Specific bacteria, like <i>Flavobacterium</i>, were significantly associated with multiple chemical components, suggesting their potential roles in chemical changes of tobacco leaves during curing. These insights highlight the complex interplay between chemical composition and bacterial dynamics, offering opportunities to optimize the curing process for enhanced tobacco quality.</p></div>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13598-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron oxide nanoparticles: biosynthesis, peroxidase-like activity, and biosafety","authors":"Yusur Ramzi Hasan,&nbsp;Fadzlie Wong Faizal Wong,&nbsp;Siti Efliza Ashari,&nbsp;Murni Halim,&nbsp;Rosfarizan Mohamad","doi":"10.1007/s00253-025-13589-w","DOIUrl":"10.1007/s00253-025-13589-w","url":null,"abstract":"<p>The rising threat of antibiotic-resistant bacterial infections has amplified the demand for alternative therapeutic strategies and efficient catalytic systems. While natural enzymes like horseradish peroxidase offer catalytic potential, their clinical use is limited by instability, high production costs, and environmental sensitivity. Iron oxide nanoparticles (Fe₃O₄ NPs) have emerged as promising alternatives, exhibiting unique physicochemical properties, magnetic responsiveness, biocompatibility, and intrinsic catalytic activity. A key advancement in this field is the adoption of green nanotechnology, which supports the eco-friendly biosynthesis of Fe₃O₄ NPs via biological systems. However, several limitations reduced catalytic activity under certain conditions. This review highlights progress in green synthesis, focusing on iron-resistant and probiotic bacteria as sustainable and scalable biogenic platforms. Compared to chemical methods, these biological routes reduce environmental impact, lower costs, and enhance nanoparticle stability and functionality. This review also addresses the factors influencing the peroxidase-like (POD) activity of NPs. The biomedical relevance of Fe₃O₄ NPs spans diverse applications, including antibacterial therapy, cancer treatment, biosensing, food safety, and enzyme-mimicking catalysis. However, despite their therapeutic promise, significant gaps remain in the biosafety and toxicity assessments of the catalytic activity of Fe₃O₄. Hence, current advancement underscores the underutilized role of bacterial strains in nanoparticle synthesis and identifies critical knowledge gaps that need to be consolidated. It calls for standardized evaluation protocols to support the safe and effective translation of Fe₃O₄ into various applications.</p><p>• <i>The green synthesis approach of Fe</i>₃O₄<i>NPs offers an eco-friendly route over other methods</i></p><p>• <i>Probiotic-mediated synthesis of Fe</i>₃O₄<i> NPs offers a sustainable and biocompatible approach</i></p><p>• <i>Fe</i>₃O₄ <i>NPs mimic POD-like activity for catalytic biomedical and environmental applications</i></p><p>• <i>POD-like activity of Fe</i>₃O₄<i>NPs boosts its antibacterial effects via ROS generation</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13589-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: VNP20009-Abvec-Igκ-MIIP suppresses ovarian cancer progression by modulating Ras/MEK/ERK signaling pathway","authors":"Qian Wang,&nbsp;Yuwen Tang,&nbsp;Ang Dai,&nbsp;Tiange Li,&nbsp;Yulin Pei,&nbsp;Zuo Zhang,&nbsp;Xinyue Hu,&nbsp;Tingtao Chen,&nbsp;Qi Chen","doi":"10.1007/s00253-025-13593-0","DOIUrl":"10.1007/s00253-025-13593-0","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13593-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveals glucose repression of citric acid catabolism in Pichia kudriavzevii","authors":"Yichao Cheng,&nbsp;Xinyi Wang,&nbsp;Di Wu,&nbsp;Yao Lu,&nbsp;Yi Qin,&nbsp;Yanlin Liu,&nbsp;Yanying Liang,&nbsp;Yuyang Song","doi":"10.1007/s00253-025-13590-3","DOIUrl":"10.1007/s00253-025-13590-3","url":null,"abstract":"<p><i>Pichia kudriavzevii</i> is a widely used yeast in the wine industry that can degrade citric acid. However, this process can be hindered by the presence of glucose through a phenomenon called carbon catabolite repression (CCR). Herein, this study determined the underlying mechanism by examining the effects of glucose on <i>P. kudriavzevii</i>. Our findings indicated that glucose inhibited the reduction of citric acid and maintained elevated levels of fatty acids and glycerophospholipids. However, the inhibition of citric acid degradation under glucose addition was related to the retarded accumulation of metabolites involved in the biosynthesis of antibiotics, propanoate metabolism, microbial metabolism in diverse environments, C5-branched dibasic acid metabolism, and metabolic pathways in diverse environments. Additionally, the integrated data revealed that citrate catabolism of <i>P. kudriavzevii</i> was remarkably repressed in response to glucose by regulating glycerophospholipid metabolism, carbon metabolism and the biosynthesis pathways of secondary metabolites. Further investigations indicated that the increase of fatty acids (e.g., alpha-linolenic and arachidic) and glycerophospholipids (e.g., dihydroxyacetone phosphate and glycerophosphocholine) under glucose addition was related to the up-regulated <i>GPD1</i>, <i>PISD</i><i>, </i><i>HIS1</i> and <i>RPIA</i> gene expressions in glycerophospholipid metabolism and the down-regulated <i>FBP1</i>, <i>MDH</i>, <i>IDH3</i>, <i>ICL1</i>, <i>ACL</i> and <i>JEN1</i> gene expressions in carbon metabolism and the biosynthesis pathways of secondary metabolites. Meantime, glucose regulated the expression of transcription factors (e.g., <i>MIG1</i> and <i>GCN4</i>) associated with three pathways, which were crucial genes of CCR regulatory networks. Overall, we uncovered the metabolic regulatory network through which CCR inhibits citric acid utilization in <i>P. kudriavzevii</i>.</p><p>• <i>Metabolic changes of P. kudriavzevii cells responding to carbon sources were observed</i></p><p>• <i>Potential genes regulating citric acid degradation contributing to CCR were screened</i></p><p>• <i>The inhibition of citric acid degradation is due to changes in the regulatory network</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13590-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}