Applied Microbiology and Biotechnology最新文献

{"title":"Oral microbiota in preschoolers with rampant caries: a matched case–control study","authors":"Yao Wu,&nbsp;Guiding Li,&nbsp;Chang-hai Lyu,&nbsp;Ni Zhou,&nbsp;Hai Ming Wong","doi":"10.1007/s00253-024-13362-5","DOIUrl":"10.1007/s00253-024-13362-5","url":null,"abstract":"<p>Rampant caries is identified by rapid onset, severe decay affecting multiple surfaces, and early pulp infection. This case–control study was conducted to investigate the disparities in oral microbiota between children affected by rampant caries and their caries-free counterparts. A total of 88 preschool children, with matched distribution of sex and age in both the case and control groups, participated in this study. Children’s oral health–related behaviors were reported by parents, salivary pH levels were assessed using a portable pen-type pH meter, and supragingival dental plaque was analyzed by 16S rRNA gene sequencing. Children with rampant caries exhibited lower salivary pH levels, poorer toothbrushing habits, and more frequent consumption of sugary snacks. <i>Veillonella</i>, enriched in caries-free children, showed a positive correlation with salivary pH levels and a negative correlation with candy consumption. Conversely, <i>Fusobacterium</i> and <i>Neisseria</i>, more abundant in children with rampant caries, positively correlated with the frequency of candy consumption. Furthermore, <i>Streptococcus mutans</i>, <i>Porphyromonas gingivalis</i>, and <i>Bacteroides acidifaciens</i> were identified as potential oral microbiome markers for differentiating preschoolers with rampant caries from their caries-free peers. <i>B. acidifaciens</i>, typically found in the gut, has been rarely reported in the field of oral health. More well-designed cohort studies are recommended to elucidate the mechanisms through which gut microbiota influences rampant caries in pediatric patients and offer insights into effective strategies for caries management in young children.</p><p><i>• Lower salivary pH levels in children with rampant caries.</i></p><p><i>• Biomarkers for predicting rampant caries.</i></p><p><i>• Impact of oral health–related behaviors on oral microbiota.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13362-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coelimycin inside out — negative feedback regulation by its intracellular precursors","authors":"Magdalena Kotowska,&nbsp;Mateusz Wenecki,&nbsp;Bartosz Bednarz,&nbsp;Jarosław Ciekot,&nbsp;Wojciech Pasławski,&nbsp;Tomasz Buhl,&nbsp;Krzysztof J. Pawlik","doi":"10.1007/s00253-024-13366-1","DOIUrl":"10.1007/s00253-024-13366-1","url":null,"abstract":"<p>Coelimycin (CPK) producer <i>Streptomyces coelicolor</i> A3(2) is a well-established model for the genetic studies of bacteria from the genus <i>Streptomyces</i>, renowned for their ability to produce a plethora of antibiotics and other secondary metabolites. Expression regulation of natural product biosynthetic gene clusters (BGCs) is highly complex, involving not only regulatory proteins, like transcription factors, but also the products of the biosynthetic pathway that may act as ligands for some regulators and modulate their activity. Here, we present the evidence that intracellular CPK precursor(s) (preCPK) is involved in a negative feedback loop repressing the CPK BGC. Moreover, we provide a characterization of the cluster-encoded efflux pump CpkF. We show that CpkF is essential for the extracellular CPK production. In order to track down which CPK compounds — intra- or extracellular — are the ones responsible for the feedback signal, a luciferase-based reporter system was applied to compare the activity of 13 CPK gene promoters in the wild-type (WT) and two mutated strains. The first strain, lacking the CPK-specific exporter CpkF (<i>ΔcpkF</i>), was unable to produce the extracellular CPK. The second one did not produce any CPK at all, due to the disruption of the CpkC polyketide synthase subunit (<i>ΔcpkC</i>). All tested promoters were strongly upregulated in <i>ΔcpkC</i> strain, while in the <i>ΔcpkF</i> strain, promoter activity resembled the one of WT. These results lead to the conclusion that the CPK polyketide acts as a silencer of its own production. Supposedly this function is exerted via binding of the preCPK by an unidentified regulatory protein.</p><p>•<i>Intracellular coelimycin precursor takes part in a negative cpk cluster regulation</i></p><p>•<i>CpkF exporter is essential for the extracellular coelimycin production</i></p><p>•<i>Simple method for the analysis of coelimycin P2 production in agar medium</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13366-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Characterization and stabilization of the α-L-fucosidase set from Lacticaseibacillus rhamnosus INIA P603","authors":"José Antonio Curiel,&nbsp;Ángela Peirotén,&nbsp;Susana Langa,&nbsp;Estela de Vega,&nbsp;Laura Blasco,&nbsp;José María Landete","doi":"10.1007/s00253-024-13370-5","DOIUrl":"10.1007/s00253-024-13370-5","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13370-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles of Janthinobacterium lividum as violacein carriers in melanoma cell treatment","authors":"Patrycja Kowalska,&nbsp;Jolanta Mierzejewska,&nbsp;Paulina Skrzeszewska,&nbsp;Aleksandra Witkowska,&nbsp;Katarzyna Oksejuk,&nbsp;Ewa Sitkiewicz,&nbsp;Mariusz Krawczyk,&nbsp;Magdalena Świadek,&nbsp;Agata Głuchowska,&nbsp;Klaudia Marlicka,&nbsp;Anna Sobiepanek,&nbsp;Małgorzata Milner-Krawczyk","doi":"10.1007/s00253-024-13358-1","DOIUrl":"10.1007/s00253-024-13358-1","url":null,"abstract":"<p>Violacein is a natural indole-derived purple pigment of microbial origin that has attracted attention for its remarkable biological properties. Due to its poor solubility in aqueous media, most studies of this pigment use extracts of the compound obtained with common solvents. Violacein is also transported in bacterial extracellular vesicles (EVs) and transferred via this type of carrier remains stable in an aqueous environment. This paper is the first to present an in-depth study of <i>Janthinobacterium lividum</i> EVs as violacein carriers. <i>J. lividum</i> EVs were studied for their contribution to violacein translocation, size, morphology and protein composition. The production of violacein encapsulated in EVs was more efficient than the intracellular production of this compound. The average size of the violacein-containing EVs was 124.07 ± 3.74 nm. Liquid chromatography-tandem mass spectrometry analysis (LC–MS/MS) revealed 932 proteins common to three independent EVs isolations. The high proportion of proteins with intracellular localisation, which are involved in many fundamental cellular processes, suggests that <i>J. lividum</i> EVs could be generated in a cell lysis model, additionally stimulated by violacein production. Using human keratinocytes and melanoma cell lines, it was confirmed that <i>J. lividum</i> EVs are able to react with and deliver their cargo to mammalian cells. The EVs-delivered violacein was shown to retain its activity against melanoma cells, and the dose and timing of treatment can be selected to target only cancer cells. The characterisation of <i>J. lividum</i> EVs, described in the following paper, represents a milestone for their future potential anticancer application.</p><p>• <i>This report focuses on the investigation of Janthinobacterium lividum EVs as a new delivery vehicle for violacein, a compound with a previously demonstrated broad spectrum of activity.</i></p><p>• <i>EVs were characterised for size, morphology and protein composition.</i></p><p>• <i>Studies on human keratinocytes and a melanoma cell model confirmed that the activity of violacein applied in the encapsulated form of EVs is similar to that of its organic solvent extract, but their production is much more environmentally friendly.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13358-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimum blue light exposure: a means to increase cell-specific productivity in Chinese hamster ovary cells","authors":"Stefanie Föller,&nbsp;Niklas Regett,&nbsp;Levin Lataster,&nbsp;Gerald Radziwill,&nbsp;Ralf Takors","doi":"10.1007/s00253-024-13363-4","DOIUrl":"10.1007/s00253-024-13363-4","url":null,"abstract":"<p>Research for biopharmaceutical production processes with mammalian cells steadily aims to enhance the cell-specific productivity as a means for optimizing total productivities of bioreactors. Whereas current technologies such as pH, temperature, and osmolality shift require modifications of the cultivation medium, the use of optogenetic switches in recombinant producer cells might be a promising contact-free alternative. However, the proper application of optogenetically engineered cells requires a detailed understanding of basic cellular responses of cells that do not yet contain the optogenetic switches. The knowhow of ideal light exposure to enable the optimum use of related approaches is missing so far. Consequently, the current study set out to find optimum conditions for IgG1 producing Chinese hamster ovary (CHO) cells which were exposed to blue LED light. Growth characteristics, cell-specific productivity using enzyme-linked immunosorbent assay, as well as cell cycle distribution using flow cytometry were analyzed. Whereas too harsh light exposure causes detrimental growth effects that could be compensated with antioxidants, a surprising boost of cell-specific productivity by 57% occurred at optimum high light doses. The increase coincided with an increased number of cells in the G1 phase of the cell cycle after 72 h of illumination. The results present a promising new approach to boost biopharmaceutical productivity of mammalian cells simply by proper light exposure without any further optogenetic engineering.</p><p><i>• Blue LED light hinders growth in CHO DP-12 cells</i></p><p><i>• Antioxidants protect to a certain degree from blue light effects</i></p><p><i>• Illumination with blue LED light raises cell-specific productivity</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13363-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Promoters for the expression of food-grade selectable markers in lactic acid bacteria and bifidobacteria","authors":"Susana Langa,&nbsp;Ángela Peirotén,&nbsp;José Antonio Curiel,&nbsp;Juan Luis Arqués,&nbsp;José María Landete","doi":"10.1007/s00253-024-13368-z","DOIUrl":"10.1007/s00253-024-13368-z","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13368-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperthermophilic xylanase and thermophilicity analysis by molecular dynamic simulation with quantum mechanics","authors":"Katewadee Boonyapakron,&nbsp;Braden Keiser,&nbsp;Kanoknart Prabmark,&nbsp;Katesuda Aiewviriyasakul,&nbsp;Nattapol Arunrattanamook,&nbsp;Aritsara Jaruwat,&nbsp;Penchit Chitnumsub,&nbsp;Jia-Yi Li,&nbsp;Tuck Seng Wong,&nbsp;Xin-Qing Zhao,&nbsp;Chen-Guang Liu,&nbsp;Dong-Qing Wei,&nbsp;Verawat Champreda","doi":"10.1007/s00253-024-13356-3","DOIUrl":"10.1007/s00253-024-13356-3","url":null,"abstract":"<p>Thermophilic xylanases catalyzing the cleavage of β-1,4-glycosidic bonds in xylan have applications in food, feed, biorefinery, and pulp industries. In this study, a hyperthermophilic endo-xylanase was obtained by further enhancement of thermal tolerance of a thermophilic GH11 xylanase originated from metagenome of bagasse pile based on rational design. Introducing N13F and Q34L to the previously reported X11P enzyme shifted the optimal working temperature to 85 °C and led to 20.7-fold improvement in thermostability at 90 °C along with a marked increase in <i>T</i>_m to 93.3 °C. X11PNQ enzyme converted xylan to prebiotic xylooligosaccharides with high specificity on xylobiose to xylohexaose and high operational stability at 85 °C, resulting in 10.3-folds yield improvement compared to the parental enzyme. Molecular dynamic simulation and quantum mechanical analysis revealed improved H-bonding networks within GH11 xylanase principal domains and greater dynamic cross-correlations. A novel thermostabilization mechanism by π-amide interaction with slightly lower interaction energy than the native H-bond, but compensated by increased occurrence frequency was firstly demonstrated for thermophilic enzymes. The enzyme represents one of the most thermostable xylanases ever reported with biotechnological potential.</p><p>• <i>Hyperthemophilic xylanase X11PNQ was obtained by rational design engineering.</i></p><p>• <i>X11PNQ showed specificity to prebiotic xylooligosaccharides (XOS) at 85 °C with improved t</i>_<i>1/2</i><i> at 90 °C.</i></p><p>• <i>Novel thermostabilization by π-amide interaction was demonstrated by MD/QM.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13356-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control Meloidogyne javanica and M. incognita","authors":"Yolanda Bel,&nbsp;Magda Galeano,&nbsp;Mireya Baños-Salmeron,&nbsp;Miguel Andrés-Antón,&nbsp;Baltasar Escriche","doi":"10.1007/s00253-024-13365-2","DOIUrl":"10.1007/s00253-024-13365-2","url":null,"abstract":"<p>The global imperative to enhance crop protection while preserving the environment has increased interest in the application of biological pesticides. <i>Bacillus thuringiensis</i> (Bt) is a Gram-positive bacterium that can produce nematicidal proteins and accumulate them in parasporal crystals. Root-knot nematodes are obligate root plant parasitic which are distributed worldwide, causing severe damages to the infested plants and, consequently, large yield reductions. In this work, we have evaluated the toxicity of the Bt crystal proteins Cry5, Cry21, App6, and Xpp55 against two root-knot nematodes belonging to the <i>Meloidogyne</i> genus (<i>M. incognita</i> and <i>M. javanica</i>). The results show that all four proteins, when solubilized, were highly toxic for both nematode species. To check the potential of using Bt strains producing nematicidal crystal proteins as biopesticides to control root-knot nematodes in the field, <i>in planta</i> assays were conducted, using two wild Bt strains which produced Cry5 or a combination of App6 and Cry5 proteins. The tests were carried out with cucumber or with tomato plants infested with <i>M. javanica</i> J2, irrigated with spore + cristal mixtures of the respective strains. The results showed that the effectiveness of the nematicidal activity was plant-dependent, as Bt was able to reduce emerged J2 in tomato plants but not in cucumber plants. In addition, the toxicity observed in the <i>in planta</i> assays was much lower than expected, highlighting the difficulty of the proteins supplied as crystals to exert their toxicity. This emphasizes the delivery of the Bt proteins as crucial for its use to control root-knot nematodes.</p><p>• <i>Solubilized Cry5, Cry21, App6 and Xpp55 Bt proteins are toxic to M. javanica.</i></p><p>• <i>Cry21 toxicity to M. incognita is similar to that of Cry5, App6, and Xpp55 proteins.</i></p><p>• <i>The Cry5 and App6 toxicities on M. javanica after Bt irrigation is crop dependent.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-024-13365-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-metabolic degradation and metabolite detection of hexabromocyclododecane by Shewanella oneidensis MR-1","authors":"Syed Bilal Shah, Yiting Wang, Naveed Anwar, Syed Zaghum Abbas, Khalid Ali Khan, Song-Mei Wang, Muhammad Wajid Ullah","doi":"10.1007/s00253-023-12905-6","DOIUrl":"https://doi.org/10.1007/s00253-023-12905-6","url":null,"abstract":"<span> <h3>Abstract</h3> <p>Hexabromocyclododecane (HBCD) is a widely used brominated flame retardant; however, it is a persistent organic pollutant as well as affects the human thyroid hormones and causes cancer. However, the degradation of HBCD has received little attention from researchers. Due to its bioaccumulative and hazardous properties, an appropriate strategy for its remediation is required. In this study, we investigated the biodegradation of HBCD using <em>Shewanella oneidensis</em> MR-1 under optimized conditions. The Box-Behnken design (BBD) was implemented for the optimization of the physical degradation parameters of HBCD. <em>S. oneidensis</em> MR-1 showed the best degradation performance at a temperature of 30 °C, pH 7, and agitation speed of 115 rpm, with an HBCD concentration of 1125 μg/L in mineral salt medium (MSM). The strain tolerated up to 2000 μg/L HBCD. Gas chromatography-mass spectrometry analysis identified three intermediates, including 2-bromo dodecane, 2,7,10-trimethyldodecane, and 4-methyl-1-decene. The results provide an insightful understanding of the biodegradation of HBCD by <em>S. oneidensis</em> MR-1 under optimized conditions and could pave the way for further eco-friendly applications.</p> </span> <span> <h3>Key points</h3> <p>• <em>HBCD biodegradation by Shewanella oneidensis</em></p> <p>• <em>Optimization of HBCD biodegradation by the Box-Behnken analysis</em></p> <p>• <em>Identification of useful metabolites from HBCD degradation</em></p> </span>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Escherichia coli in electrogenic biofilm on activated carbon in microbial fuel cell.","authors":"Younggun Yoon, Azilah Abd Aziz, In Seop Chang, Bongkyu Kim","doi":"10.1007/s00253-023-12829-1","DOIUrl":"10.1007/s00253-023-12829-1","url":null,"abstract":"<p><p>For a better understanding of the distribution of depth-dependent electrochemically active bacteria at in the anode zone, a customized system in a microbial fuel cell (MFC) packed with granular activated carbon (GAC) was developed and subsequently optimized via electrochemical tests. The constructed MFC system was sequentially operated using two types of matrice solutions: artificially controlled compositions (i.e., artificial wastewater, AW) and solutions obtained directly from actual sewage-treating municipal plants (i.e., municipal wastewater, MW). Notably, significant difference(s) of system efficiencies between AW or MW matrices were observed via performance tests, in that the electricity production capacity under MW matrices is < 25% that of the AW matrices. Interestingly, species of Escherichia coli (E. coli) sampled from the GAC bed (P1: deeper region in GAC bed, P2: shallow region of GAC near electrolytes) exhibited an average relative abundance of 75 to 90% in AW and a relative abundance of approximately 10% in MW, while a lower relative abundance of E. coli was found in both the AW and MW anolyte samples (L). Moreover, similar bacterial communities were identified in samples P1 and P2 for both the AW and MW solutions, indicating a comparable distribution of bacterial communities over the anode area. These results provide new insights into E. coli contribution in power production for the GAC-packed MFC systems (i.e., despite the low contents of Geobacter (> 8%) and Shewanella (> 1%)) for future applications in sustainable energy research. KEY POINTS: • A microbial community analysis for depth-dependence in biofilm was developed. • The system was operated with two matrices; electrochemical performance was assessed. • E. coli spp. was distinctly found in anode zone layers composed of activated carbon.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"108 1","pages":"52"},"PeriodicalIF":5.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}