Applied Microbiology and Biotechnology最新文献

{"title":"Phage/nanoparticle cocktails for a biocompatible and environmentally friendly antibacterial therapy.","authors":"Mateusz Wdowiak, Sada Raza, Mateusz Grotek, Rafał Zbonikowski, Julita Nowakowska, Maria Doligalska, Ningjing Cai, Zhi Luo, Jan Paczesny","doi":"10.1007/s00253-025-13526-x","DOIUrl":"10.1007/s00253-025-13526-x","url":null,"abstract":"<p><p>Antibiotic resistance continues to rise, necessitating alternative strategies. Bacteriophages have emerged as promising natural antibacterial agents, offering a targeted approach to combating bacterial infections. Combining bacteriophages with nanoparticles presents a novel approach that could enhance antibacterial potency while reducing the risk of resistance. While phage/antibiotic cocktails are widely explored to enhance antibacterial efficacy and prevent resistance, research on phage/nanoparticle combinations remains limited. We explore the synergy between green tea extract-capped silver nanoparticles (G-TeaNPs) and bacteriophages in combating pathogenic bacteria (methicillin-resistant Staphylococcus aureus, Salmonella enterica). G-TeaNPs show minimal antiphage activity, ensuring compatibility in phage-NP formulations. These combinations significantly reduce bacterial counts in a short time (only 3 h), e.g., S. aureus survival is around 30% after incubation with just 0.001 mg/mL of G-TeaNPs, while G-TeaNPs and phages alone result in around 80% and 70% survival, respectively. Cytotoxicity tests against eukaryotic 3T3 NIH fibroblast cells confirm biocompatibility at effective concentrations. Additionally, we examine G-TeaNPs' impact on the free-living protist Acanthamoeba castellanii. Both green tea extract and G-TeaNPs can reduce A. castellanii cell counts by 80%, but only at high concentrations. Microscopy revealed nanoparticle uptake by amoebae, causing intracellular accumulation and vacuolization, while green tea extract induced similar changes without uptake. Our findings highlight G-TeaNPs as safe, effective agents in phage/nanoparticle antibacterial formulations with dual antimicrobial and amoebicidal properties for therapeutic and environmental applications. KEYPOINTS: • Silver nanoparticles synthesized with tea extracts (G-TeaNPs) have a minimal effect on the tested viruses. • Combining G-TeaNP with bacteriophages offers new-generation antibacterial cocktails. • Green tea extracts and AgNPs present concentration-dependent anti-amoebic activity.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"129"},"PeriodicalIF":3.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective simultaneous removal of 17β-estradiol and tetracycline by a novel Alkalibacterium strain: characteristics, mechanisms, and application in livestock wastewater treatment.","authors":"Yaqiong Wang, Huike Ye, Wenxin Li, Hanye Ju, Yaodong He, Shuo Yang, Haijiao Liu, Xiaodong Ma, Mohan Bai, Lixia Zhao, Yang Sun, Xiaojing Li","doi":"10.1007/s00253-025-13516-z","DOIUrl":"10.1007/s00253-025-13516-z","url":null,"abstract":"<p><p>The environmental risk posed by 17β-estradiol (E2) and tetracycline (TC) contamination within livestock wastewater has been widely concerned. Especially, the co-occurrence of these pollutants poses a tremendous challenge to their efficient bioremediation, highlighting the need for strains capable of simultaneous E2 and TC removal. In this study, a novel strain of Alkalibacterium sp. AEPI-S25 was successfully isolated from the sediments of Qinghai Lake, demonstrating the ability to remove 89.91% of 20 mg L^-1 E2 and nearly 100% of 20 mg L^-1 TC simultaneously within 5 days. AEPI-S25 exhibited remarkable environmental adaptability and maintained high simultaneous removal efficiency under various stress conditions and the presence of two typical livestock wastewater samples. Based on Ultra-Performance Liquid Chromatography coupled with Orbitrap High-Resolution Mass Spectrometry (UPLC-Orbitrap-HRMS) analysis, dehydrogenation and monooxygenation were identified as the key steps in the removal pathways of E2 and TC, respectively. Whole-genome sequencing further identified the potential E2/TC removal genes, and the detected potential E2-dehydrogenation (S25_gene0393) and TC-monooxygenation (S25_gene0878) genes were subsequently validated through transcription analysis and heterologous expression. Notably, S25_gene0878 exhibited significant differences from the well-characterized TC removal gene TetX, extending a new understanding of the bacterial TC removal mechanism. Overall, this study provides the first report of a single microbial strain capable of simultaneously removing E2 and TC, offering valuable insights for the application of microbial technologies in addressing typical E2-TC combined pollution in livestock wastewater. KEY POINTS: • AEPI-S25 can remove nearly 90% and 100% of 20 mg L-1 E2 and TC within 5 days • Key E2 (S25_gene0393) and TC (S25_gene0878) removal genes were cloned and expressed • A novel TC-monooxygenase gene distinct from TetX was discovered within the strain.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"127"},"PeriodicalIF":3.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell walls of filamentous fungi – challenges and opportunities for biotechnology","authors":"Katharina J. Ost,&nbsp;Mounashree Student,&nbsp;Stefan Cord-Landwehr,&nbsp;Bruno M. Moerschbacher,&nbsp;Arthur F. J. Ram,&nbsp;Mareike E. Dirks-Hofmeister","doi":"10.1007/s00253-025-13512-3","DOIUrl":"10.1007/s00253-025-13512-3","url":null,"abstract":"<p>The cell wall of filamentous fungi is essential for growth and development, both of which are crucial for fermentations that play a vital role in the bioeconomy. It typically has an inner rigid core composed of chitin and beta-1,3-/beta-1,6-glucans and a rather gel-like outer layer containing other polysaccharides and glycoproteins varying between and within species. Only a fraction of filamentous fungal species is used for the biotechnological production of enzymes, organic acids, and bioactive compounds such as antibiotics in large amounts on a yearly basis by precision fermentation. Most of these products are secreted into the production medium and must therefore pass through fungal cell walls at high transfer rates. Thus, cell wall mutants have gained interest for industrial enzyme production, although the causal relationship between cell walls and productivity requires further elucidation. Additionally, the extraction of valuable biopolymers like chitin and chitosan from spent fungal biomass, which is predominantly composed of cell walls, represents an underexplored opportunity for circular bioeconomy. Questions persist regarding the effective extraction of these biopolymers from the cell wall and their repurposing in valorization processes. This review aims to address these issues and promote further research on understanding the cell walls in filamentous fungi to optimize their biotechnological use.</p><p><i>• The highly complex cell walls of filamentous fungi are important for biotechnology.</i></p><p><i>• Cell wall mutants show promising potential to improve industrial enzyme secretion.</i></p><p><i>• Recent studies revealed enhanced avenues for chitin/chitosan from fungal biomass.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13512-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of metagenomic analysis on the discovery of novel endolysins","authors":"Nikolaos Georgakis,&nbsp;Georgios E. Premetis,&nbsp;Panagiota Pantiora,&nbsp;Christina Varotsou,&nbsp;Charoutioun S. Bodourian,&nbsp;Nikolaos E. Labrou","doi":"10.1007/s00253-025-13513-2","DOIUrl":"10.1007/s00253-025-13513-2","url":null,"abstract":"<p>Metagenomics has revolutionized enzyme discovery by enabling the study of genetic material directly from environmental samples, bypassing the need for microbial cultivation. This approach is particularly effective for identifying novel endolysins, phage-derived enzymes with antibacterial properties suited for therapeutic and industrial applications. Diverse ecosystems, such as biofilms, human microbiome, hot springs, and geothermal areas, serve as rich reservoirs for endolysins with traits like thermostability, broad-spectrum activity, specificity and resistance to harsh conditions. Functional metagenomics, complemented by bioinformatics, enables the discovery and annotation of previously uncharacterized endolysins. Examples of endolysins discovered from metagenomics analysis are discussed. Despite the challenges of analyzing complex microbial ecosystems and isolating target genes, metagenomics holds immense potential for uncovering innovative endolysins, paving the way for developing new biotechnological applications.</p><p><i>• Endolysins offer antibacterial potential for therapeutic and industrial use.</i></p><p><i>• Metagenomics enables discovery of novel endolysins from diverse ecosystems.</i></p><p><i>• Advances in tools and methods have accelerated novel endolysins discovery.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13513-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering factors influencing planktonic and sedimentary microbial assembly processes in Midwest salinity lakes","authors":"Jincheng Hu,&nbsp;Tianyi Chen,&nbsp;Chun-Ang Lian,&nbsp;Li Wang,&nbsp;Weiqin Zhuang,&nbsp;Ke Yu","doi":"10.1007/s00253-025-13498-y","DOIUrl":"10.1007/s00253-025-13498-y","url":null,"abstract":"<p>The salt lake ecosystem, characterized by extreme environmental gradients, harbors microbes that uniquely adapt to high salt stress through natural selection. However, how abiotic and biotic factors shape the microbial community assembly in Yuncheng Salt Lakes remains unclear. Here, we investigated the assembly processes and meta co-occurrence patterns of microbiota in both water and sediment sampled from 14 distinct wide range of salinity lakes in the Shanxi Yuncheng area, Midwest of China, using 16S rRNA and 18S rRNA gene sequencing technology combined with multivariate ecological and statistical methods. Habitat differentiation led to the differences in microbial diversity, co-occurrence patterns, and community assembly between sedimentary and planktonic communities. Sedimentary prokaryotes were more shaped by deterministic processes than planktonic bacterial communities. Salinity was a major abiotic factor influencing the balance between stochastic and deterministic processes in both sediment and water. Enhanced microbial interactions within sediments exhibited a more prominent impact in shaping community assembly, as indicated by the stronger association between network-inferred species and prokaryotic βNTI. Moreover, we revealed significant differences in how core species concerning βNTI responded to biotic and abiotic factors. Our findings elucidated the ecological process underlying microbial communities in Yuncheng Salt Lakes and shed light on the mechanism of microorganisms to maintain community complexity and diversity in the extreme environment.</p><p><i>• Sedimentary prokaryotes were more shaped by deterministic processes than planktonic prokaryotic communities.</i></p><p><i>• Salinity was a major factor influencing the balance between stochastic and deterministic process.</i></p><p><i>• Inter-domain and intra-domain symbiotic interactions within sedimentary communities represent key biotic factors influencing their community assembly.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13498-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substrate utilization and secondary metabolite biosynthesis in the phylum Planctomycetota","authors":"Madeleine Kündgen,&nbsp;Christian Jogler,&nbsp;Nicolai Kallscheuer","doi":"10.1007/s00253-025-13514-1","DOIUrl":"10.1007/s00253-025-13514-1","url":null,"abstract":"<p>The phylum <i>Planctomycetota</i> is changing our understanding of bacterial metabolism, driving critical biogeochemical processes through the transformation of complex polymeric substrates into valuable bioactive compounds. Sophisticated methods for cultivation, genome sequencing and genetic strain engineering developed in the last two decades have stimulated detailed studies on cell propagation, metabolic capabilities and potential applications of phylum members beyond the mere isolation and characterization of novel taxa. This review synthesizes recent advances in understanding the <i>Planctomycetota</i> physiology with a focus on the degradation of phototroph-derived polysaccharides, anaerobic ammonium oxidation (anammox) and biosynthesis of secondary metabolites. New data especially collected over the last 5 years justifies more intensive research of the yet uncharacterized pathways of substrate uptake and utilization, as well as genome mining-assisted bioprospection to exploit the phylum's chemical repertoire.</p><p>• <i>Planctomycetes can degrade high-molecular-weight sugars produced by algae</i></p><p>• <i>Anaerobic ammonium oxidation (anammox) is used in technical applications</i></p><p>• <i>The first secondary metabolites were discovered in the last 5 years</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13514-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a novel alginate lyase Alg0392 with organic solvent-tolerance from Alteromonas sp. A1-6","authors":"Tong Liang,&nbsp;Jing Chen,&nbsp;Jing Li,&nbsp;Ming-li Dong,&nbsp;Zhenggang Han,&nbsp;Feng-juan Shan,&nbsp;Xue-wang Gao,&nbsp;Da-zhong Yan","doi":"10.1007/s00253-025-13506-1","DOIUrl":"10.1007/s00253-025-13506-1","url":null,"abstract":"<p>Enzymatic depolymerization of seaweed polysaccharides aroused great interest in the production of functional oligosaccharides and fermentable sugars. Alginate lyase Alg0392, a potential novel member of the polysaccharide lyase PL17 family, was cloned from <i>Alteromonas</i> sp. A1-6. The enzymatic properties, kinetic parameters, and hydrolytic products of Alg0392 were systematically characterized. Especially, the recombinant enzyme Alg0392 showed excellent tolerance to organic reagents. When treated with 5 mmol/L of TritonX-100 or 20%(v/v) of methanol, its relative enzyme activity could be maintained at more than 70%. The recombinant enzyme has a substrate preference for poly (β-D-mannuronic acid). The products of alginate hydrolysis catalyzed by Alg0392 are mainly monosaccharides, disaccharides, and trisaccharides. The products generated by the degradation of polymannuronic acid (polyM) are mainly monosaccharides. So Alg0392 is a polymannuronate cleaving enzyme. It has excellent organic solvent-tolerance and possesses both endo- and exo-glycosidase activities towards alginate. These unique properties make the recombinant enzyme Alg0392 more advantageous for the future industrial production of biofuels and the preparation of alginate oligosaccharides.</p><p>•<i> Alg0392 is a bifunctional alginate lyase with exolytic and endolytic cleavage activity.</i></p><p>•<i> Alg0392 exhibits excellent organic solvent tolerance.</i></p><p>•<i> The enzymatic hydrolysates of Alg0392 exhibit antioxidant activity.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13506-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of menaquinone- 7 production through immobilization with hydrogel-based porous membranes","authors":"Qiu-Hua Zhang,&nbsp;Zheng Wang,&nbsp;Yao-Qiang Wang,&nbsp;Man-Lu Liu,&nbsp;Hai-Jia Su","doi":"10.1007/s00253-025-13493-3","DOIUrl":"10.1007/s00253-025-13493-3","url":null,"abstract":"<p>The industrial production of menaquinone-7 (MK-7) by <i>Bacillus subtilis</i> has been historically constrained by significant challenges in bioprocess efficiency. To address these limitations, we explored an innovative immobilization strategy utilizing a porous thin-film hydrogel system. Specifically, we developed a novel porous thin-film PVA + B@Ca hydrogel immobilization method that fundamentally transforms cell encapsulation and fermentation dynamics. The comparison between PVA + B@Ca hydrogel immobilized cells and free cells in fermentation demonstrated a significant increase in MK-7 yield from 32.76 ± 1.92 to 48.33 ± 2.92 mg/L, as well as a reduction of the fermentation duration from 48 to 24 h. Additionally, the immobilized cells demonstrated good stability during continuous fermentation, resulting in a space–time yield of MK-7 that increased to 2.0 mg/L·h, which was five times higher than that achieved with free-cell fermentation. Mechanistic insights revealed through microscopic analysis highlight the transformative nature of the hydrogel immobilization: The PVA + B@Ca hydrogel’s porous structure creates a protective microenvironment that mitigates cellular stress and maintains optimal metabolic conditions. These findings represent a paradigm shift in understanding cellular immobilization, demonstrating how strategic encapsulation can fundamentally enhance MK-7 fermentation biotechnology.</p><p><i>• A novel hydrogel immobilization method was developed for MK- 7 production</i>.</p><p><i>• The use of immobilized cells gave a fivefold improvement in the space–time yield</i>.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13493-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermally adapted Escherichia coli keeps transcriptomic response during temperature upshift exposure","authors":"Gilberto Pérez-Morales,&nbsp;Karla V. Martínez-Conde,&nbsp;Luis Caspeta,&nbsp;Enrique Merino,&nbsp;Miguel A. Cevallos,&nbsp;Guillermo Gosset,&nbsp;Alfredo Martinez","doi":"10.1007/s00253-025-13495-1","DOIUrl":"10.1007/s00253-025-13495-1","url":null,"abstract":"<p>The heat shock response is a cellular protection mechanism against sudden temperature upshifts extensively studied in <i>Escherichia coli</i>. However, the effects of thermal evolution on this response remain largely unknown. In this study, we investigated the early and late physiological and transcriptional responses to temperature upshift in a thermotolerant strain under continuous culture conditions. Adaptive laboratory evolution was performed on a metabolically engineered <i>E. coli</i> strain (JU15), designed for <span>d</span>-lactic acid production, to enable cellular growth and fermentation of glucose at 45 °C in batch cultures. The resulting homofermentative strain, ECL45, successfully adapted to 45 °C in a glucose-mineral medium at pH 7 under non-aerated conditions. The thermal-adapted ECL45 retained the parental strain’s high volumetric productivity and product/substrate yield. Genomic sequencing of ECL45 revealed eight mutations, including one in a non-coding region and six within the coding regions of genes associated with metabolic, transport, and regulatory functions. Transcriptomic analysis comparing the evolved strain with its parental counterpart under early and late temperature upshifts indicated that the adaptation involved a controlled stringent response. This mechanism likely contributes to the strain’s ability to maintain growth capacity at high temperatures.</p><p>• <i>The temperature upshift response of a thermally adapted strain in continuous culture was studied for the first time.</i></p><p>• <i>Genomic analyses revealed the presence of a double point mutation in the spoT gene.</i></p><p>• <i>The thermally adapted strain maintained underexpression of the spoT gene at high temperatures.</i></p><p>• <i>Supplementation of 0.15 g/L of hydrolyzed protein favored thermal adaptation at 45 °C.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13495-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic regulation of histone methyltransferase SUV39H1 on the expression of recombinant protein in CHO cells","authors":"Lu-Lu Yang,&nbsp;Miao Zhang,&nbsp;Jia-Liang Guo,&nbsp;Ming-Ming Han,&nbsp;Le-Le Qiu,&nbsp;Li-Jie Gao,&nbsp;Liu-Su Cui,&nbsp;Xiao-Yin Wang,&nbsp;Tian-Yun Wang,&nbsp;Yan-Long Jia","doi":"10.1007/s00253-025-13509-y","DOIUrl":"10.1007/s00253-025-13509-y","url":null,"abstract":"<div><h3>Abstract</h3><p>Histone methylation–mediated epigenetic modification significantly influences gene transcription and expression regulation. This study examined the effects of histone 3 lysine 9 trimethylation (H3K9me3) methyltransferase SUV39H1 and its specific inhibitor chaetocin on recombinant protein expression in Chinese hamster ovary (CHO) cells. Results indicated that stable SUV39H1-knockdown CHO cells exhibited reduced H3K9me3 levels while showing increased expression of recombinant adalimumab (rADM) and human serum albumin (rHSA) by approximately 45% and 136%, respectively. Furthermore, treatment with 20 nM chaetocin, a SUV39H1-specific inhibitor, enhanced expression of enhanced green fluorescent protein (EGFP), rADM, and rHSA in CHO cells. These findings demonstrate that both stable SUV39H1 knockdown and pharmacological inhibition through chaetocin effectively reduce H3K9me3 modification levels in CHO cells while significantly boosting recombinant protein production. The results strongly suggest SUV39H1’s critical regulatory role in recombinant protein expression within CHO cell systems. This research establishes a methodological foundation for developing engineered cell lines and optimizing high-efficiency CHO expression systems through cell engineering approaches.</p><h3>Key points</h3><p><i>•SUV39H1 knockdown boosted recombinant protein expression and decreased H3 K9 me3 levels.</i></p><p><i>•Treatment with the SUV39H1-specific inhibitor chaetocin (20 nM) enhanced recombinant protein expression.</i></p><p><i>•It provides a basis for developing efficient epigenetically regulated CHO expression systems.</i></p></div>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13509-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}