Genomically-selected antifungal Bacillaceae strains improve wheat yield and baking quality.

IF 3.9 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Alejo Casal, Fernán Oscar Gizzi, Sol Agostina Figueroa, Tomás Denis Petitti, Facundo Ferragutti, Jimena Gaido, Mariano Alberto Torres Manno, Gabriel Céccoli, Luciana Paoletti, Christopher Dunlap, Lucas Damián Daurelio, Martín Espariz
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Abstract

Soil microbial diversity degradation through agricultural intensification necessitates sustainable alternatives. This study employed genomic and phenotypic approaches to characterize wheat rhizosphere-associated Bacillaceae for agricultural applications. Initial screening of 576 sporulating isolates for antifungal activity against Fusarium graminearum, followed by RAPD analysis, identified 39 distinct genetic profiles, out of which 15 were classified in Bacillus amyloliquefaciens or Priestia megaterium groups by 16S RNA sequence. Whole-genome sequencing of selected strains enabled precise taxonomic classification and comprehensive trait prediction using in silico tools. Genomic mining revealed strain-specific distributions of beneficial traits, including antimicrobial compound production pathways and plant growth-promoting characteristics. Phenotypic validation confirmed key predicted traits while uncovering additional functionalities not detected in silico. Integration of kernel bioassays, pot experiments, and field trials identified Bacillus velezensis ZAV-W70 and P. megaterium ZAV-W64 as promising biofertilizer and biocontrol candidates, demonstrating enhanced yield without fungicides and improved bread-making quality, respectively. These findings highlight the value of combining genomic analysis with traditional screening methods for developing effective agricultural biologicals, contributing to sustainable wheat production practices. KEY POINTS: • Rhizosphere Bacillaceae strains show dual plant growth promotion and biocontrol • B. velezensis ZAV-W70 and P. megaterium ZAV-W64 increase wheat yield • ZAV-W64 increases bread-making quality including total gluten and alveograph W.

芽孢杆菌科抗真菌菌株提高小麦产量和烘焙品质。
农业集约化导致的土壤微生物多样性退化需要可持续的替代方案。本研究采用基因组和表型方法对小麦根际相关杆菌科进行了农业应用鉴定。对576株产孢分离株进行抗真菌活性初步筛选,随后进行RAPD分析,鉴定出39个不同的遗传图谱,其中15个通过16S RNA序列可归为解淀粉芽孢杆菌或特大镰刀菌群。所选菌株的全基因组测序可以使用计算机工具进行精确的分类和全面的性状预测。基因组挖掘揭示了菌株特有的有益性状分布,包括抗菌化合物生产途径和植物生长促进特性。表型验证证实了关键的预测性状,同时发现了在计算机中未检测到的其他功能。综合稻种生物测定、盆栽试验和田间试验,velezensis芽孢杆菌ZAV-W70和P. megaterium芽孢杆菌ZAV-W64是有前途的生物肥料和生物防治候选菌,分别证明了在不使用杀菌剂的情况下提高产量和改善面包质量。这些发现突出了将基因组分析与传统筛选方法结合起来开发有效的农业生物制剂的价值,有助于可持续小麦生产实践。•根际芽胞杆菌科菌株具有促进植物生长和生物防治的双重作用•velezensis ZAV-W70和P. megaterium ZAV-W64提高小麦产量•ZAV-W64提高面包品质,包括总面筋和alveograph W。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
10.00
自引率
4.00%
发文量
535
审稿时长
2 months
期刊介绍: Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.
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