Applied Microbiology and Biotechnology最新文献

{"title":"Genetic basis of biofilm formation and salt adaptation in the plant-beneficial strain Stutzerimonas stutzeri MJL19.","authors":"Verónica Pérez-Padilla, María Antonia Molina-Henares, Zulema Udaondo, María Isabel Ramos-González, Manuel Espinosa-Urgel","doi":"10.1007/s00253-025-13523-0","DOIUrl":"10.1007/s00253-025-13523-0","url":null,"abstract":"<p><p>Stutzerimonas stutzeri MJL19 represents a potential candidate for agrobiotechnological applications in regions affected by soil salinization, given its protective effects on plants under saline stress. This strain forms biofilms on some abiotic surfaces and on plant roots, a trait that influences the colonization and persistence capacities of bacteria in the rhizosphere. However, the mechanistic basis for the multicellular lifestyle of S. stutzeri MJL19 and its connection with the adaptation to saline conditions had not been explored. Analysis of the genome of MJL19 has allowed the identification of two gene clusters involved in the synthesis of exopolysaccharides (cellulose and a species-specific polymer). Deletion of either or both gene clusters exposed their differential roles on abiotic and biotic surfaces and phenotypic changes in response to increasing salt concentrations. Expression of both clusters is regulated by the two-component system GacS/GacA, as evidenced by analysis of a gacS mutant obtained by random transposon mutagenesis. This mutant also shows altered levels of the intracellular second messenger cyclic diguanylate (c-di-GMP), which is key in the transition between free-living and sessile lifestyles. Results also suggest the existence of regulatory interconnections between exopolysaccharide synthesis genes, and of these with c-di-GMP turnover, which is in turn modulated by the presence of NaCl. GacS is required for this response to varying salt concentrations. We also describe two additional elements that influence c-di-GMP levels and the response to salt: the gene katE, encoding catalase HP-II, and a gene that encodes a protein of the lipoteichoic acid synthases family. KEY POINTS: • GacS controls c-di-GMP levels and EPS synthesis in S. stutzeri MJL19 in response to salt. • Regulation of EPS genes is interconnected and linked to c-di-GMP turnover. • The catalase KatE influences c-di-GMP levels.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"130"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-aeration for improving anaerobic treatment and biogas production from organic pollutants.","authors":"Luís Costa, M Salomé Duarte, Carla Pereira Magalhães, M Alcina Pereira, Ana Júlia Cavaleiro","doi":"10.1007/s00253-025-13519-w","DOIUrl":"10.1007/s00253-025-13519-w","url":null,"abstract":"<p><p>Anaerobic digestion (AD) is a well-established method for waste/wastewater treatment and biogas production, but challenges remain in improving its performance, particularly for toxic/inhibitory organic compounds such as lipids, hydrocarbons, polyphenols, pharmaceuticals, and other pollutants. Micro-aeration, which involves the controlled introduction of small amounts of oxygen, has emerged as a promising strategy to enhance microbial activity, stimulate the degradation of challenging compounds, and improve methane yields. This review addresses the different strategies used for effective oxygen dosing, measurement, and control, while also delving into the bioenergetics of the coexisting anaerobic and micro-aerobic pathways. Studies demonstrating the potential of micro-aeration to enhance the anaerobic treatment of recalcitrant organic pollutants, such as BTEX (benzene, toluene, ethylbenzene, and xylene) and pharmaceutical compounds, are reviewed. Several works use micro-aeration as a pretreatment, while those implementing it directly within bioreactors typically apply it intermittently. Nevertheless, in most cases, the application of micro-aeration is guided by a \"trial-and-error\" approach, and a systematic understanding of optimal strategies and dosing for different classes of pollutants remains lacking. The review also explores the diverse roles of micro-aeration in the AD of lipids, highlighting key microorganisms and underlying mechanisms that drive these processes, for instance, the role of facultative anaerobes in converting oleate into palmitate and protecting methanogenic communities. Finally, this work highlights future directions and remaining challenges in applying micro-aeration for the anaerobic treatment of organic pollutants. KEY POINTS: AD of lipids, hydrocarbons, dyes, pharmaceuticals, and other pollutants is challenging. Micro-aeration reshapes microbial communities and enhances pollutant degradation. Effective micro-aeration depends on several factors and is not fully mastered.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"131"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of Metschnikowia pulcherrima: a dive into the genomic and safety characterization of four plant-associated strains.","authors":"Ilaria Larini, Massimo Ferrara, Eleonora Troiano, Veronica Gatto, Giuseppina Mulè, Nicola Vitulo, Vittorio Capozzi, Elisa Salvetti, Giovanna E Felis, Sandra Torriani","doi":"10.1007/s00253-025-13515-0","DOIUrl":"10.1007/s00253-025-13515-0","url":null,"abstract":"<p><p>Metschnikowia pulcherrima includes strains of applied agro-food interest, particularly due to the antimicrobial activity against plant pathogens, contribution to the aroma of fermented beverages, and preliminary evidence related to probiotic activity. This biotechnological relevance sheds new light of interest on the biology of this yeast. To better understand and expand its biotechnological potential and applicability, the genomes of M. pulcherrima NRRL Y-7111^T, NRRL Y-48695, CBS 10357, and NRRL Y-48712 were sequenced, and de-novo assembled. Between 10,671 and 14,548 genes were predicted and the cooperative genomic analyses were integrated with experimental assessments relating to traits relevant for biotechnological application and safety. In silico and in vitro safety assessment revealed intermediate sensitivity for itraconazole; furthermore, variants of the genes related to pulcherrimin production and transport were found in all the genomes. Moreover, an arsenal of carbohydrate-active enzymes (CAZymes) was unravelled, and their predicted localization was investigated. This study expands the body of knowledge on M. pulcherrima, including traits relevant for defining its safety as a bioresource, which is a pivotal aspect for its possible inclusion in the European Food Safety Authority (EFSA) Qualified Presumption of Safety (QPS) list and its application in REgulated food/feed PROducts (REPRO) both in the European Union & aligned European countries. KEY POINTS: • A pipeline for genomic characterisation and safety assessment of unconventional yeasts, using M. pulcherrima as a model species was developed. • M. pulcherrima strains can be considered safe and safety data can be used to develop a body of knowledge to include M. pulcherrima in EFSA QPS list. • Analysis of the predicted localization of CAZymes allowed the detection of compounds as potential biological control agents.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"128"},"PeriodicalIF":3.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage/nanoparticle cocktails for a biocompatible and environmentally friendly antibacterial therapy.","authors":"Mateusz Wdowiak, Sada Raza, Mateusz Grotek, Rafał Zbonikowski, Julita Nowakowska, Maria Doligalska, Ningjing Cai, Zhi Luo, Jan Paczesny","doi":"10.1007/s00253-025-13526-x","DOIUrl":"10.1007/s00253-025-13526-x","url":null,"abstract":"<p><p>Antibiotic resistance continues to rise, necessitating alternative strategies. Bacteriophages have emerged as promising natural antibacterial agents, offering a targeted approach to combating bacterial infections. Combining bacteriophages with nanoparticles presents a novel approach that could enhance antibacterial potency while reducing the risk of resistance. While phage/antibiotic cocktails are widely explored to enhance antibacterial efficacy and prevent resistance, research on phage/nanoparticle combinations remains limited. We explore the synergy between green tea extract-capped silver nanoparticles (G-TeaNPs) and bacteriophages in combating pathogenic bacteria (methicillin-resistant Staphylococcus aureus, Salmonella enterica). G-TeaNPs show minimal antiphage activity, ensuring compatibility in phage-NP formulations. These combinations significantly reduce bacterial counts in a short time (only 3 h), e.g., S. aureus survival is around 30% after incubation with just 0.001 mg/mL of G-TeaNPs, while G-TeaNPs and phages alone result in around 80% and 70% survival, respectively. Cytotoxicity tests against eukaryotic 3T3 NIH fibroblast cells confirm biocompatibility at effective concentrations. Additionally, we examine G-TeaNPs' impact on the free-living protist Acanthamoeba castellanii. Both green tea extract and G-TeaNPs can reduce A. castellanii cell counts by 80%, but only at high concentrations. Microscopy revealed nanoparticle uptake by amoebae, causing intracellular accumulation and vacuolization, while green tea extract induced similar changes without uptake. Our findings highlight G-TeaNPs as safe, effective agents in phage/nanoparticle antibacterial formulations with dual antimicrobial and amoebicidal properties for therapeutic and environmental applications. KEYPOINTS: • Silver nanoparticles synthesized with tea extracts (G-TeaNPs) have a minimal effect on the tested viruses. • Combining G-TeaNP with bacteriophages offers new-generation antibacterial cocktails. • Green tea extracts and AgNPs present concentration-dependent anti-amoebic activity.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"129"},"PeriodicalIF":3.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective simultaneous removal of 17β-estradiol and tetracycline by a novel Alkalibacterium strain: characteristics, mechanisms, and application in livestock wastewater treatment.","authors":"Yaqiong Wang, Huike Ye, Wenxin Li, Hanye Ju, Yaodong He, Shuo Yang, Haijiao Liu, Xiaodong Ma, Mohan Bai, Lixia Zhao, Yang Sun, Xiaojing Li","doi":"10.1007/s00253-025-13516-z","DOIUrl":"10.1007/s00253-025-13516-z","url":null,"abstract":"<p><p>The environmental risk posed by 17β-estradiol (E2) and tetracycline (TC) contamination within livestock wastewater has been widely concerned. Especially, the co-occurrence of these pollutants poses a tremendous challenge to their efficient bioremediation, highlighting the need for strains capable of simultaneous E2 and TC removal. In this study, a novel strain of Alkalibacterium sp. AEPI-S25 was successfully isolated from the sediments of Qinghai Lake, demonstrating the ability to remove 89.91% of 20 mg L^-1 E2 and nearly 100% of 20 mg L^-1 TC simultaneously within 5 days. AEPI-S25 exhibited remarkable environmental adaptability and maintained high simultaneous removal efficiency under various stress conditions and the presence of two typical livestock wastewater samples. Based on Ultra-Performance Liquid Chromatography coupled with Orbitrap High-Resolution Mass Spectrometry (UPLC-Orbitrap-HRMS) analysis, dehydrogenation and monooxygenation were identified as the key steps in the removal pathways of E2 and TC, respectively. Whole-genome sequencing further identified the potential E2/TC removal genes, and the detected potential E2-dehydrogenation (S25_gene0393) and TC-monooxygenation (S25_gene0878) genes were subsequently validated through transcription analysis and heterologous expression. Notably, S25_gene0878 exhibited significant differences from the well-characterized TC removal gene TetX, extending a new understanding of the bacterial TC removal mechanism. Overall, this study provides the first report of a single microbial strain capable of simultaneously removing E2 and TC, offering valuable insights for the application of microbial technologies in addressing typical E2-TC combined pollution in livestock wastewater. KEY POINTS: • AEPI-S25 can remove nearly 90% and 100% of 20 mg L-1 E2 and TC within 5 days • Key E2 (S25_gene0393) and TC (S25_gene0878) removal genes were cloned and expressed • A novel TC-monooxygenase gene distinct from TetX was discovered within the strain.</p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":"127"},"PeriodicalIF":3.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell walls of filamentous fungi – challenges and opportunities for biotechnology","authors":"Katharina J. Ost,&nbsp;Mounashree Student,&nbsp;Stefan Cord-Landwehr,&nbsp;Bruno M. Moerschbacher,&nbsp;Arthur F. J. Ram,&nbsp;Mareike E. Dirks-Hofmeister","doi":"10.1007/s00253-025-13512-3","DOIUrl":"10.1007/s00253-025-13512-3","url":null,"abstract":"<p>The cell wall of filamentous fungi is essential for growth and development, both of which are crucial for fermentations that play a vital role in the bioeconomy. It typically has an inner rigid core composed of chitin and beta-1,3-/beta-1,6-glucans and a rather gel-like outer layer containing other polysaccharides and glycoproteins varying between and within species. Only a fraction of filamentous fungal species is used for the biotechnological production of enzymes, organic acids, and bioactive compounds such as antibiotics in large amounts on a yearly basis by precision fermentation. Most of these products are secreted into the production medium and must therefore pass through fungal cell walls at high transfer rates. Thus, cell wall mutants have gained interest for industrial enzyme production, although the causal relationship between cell walls and productivity requires further elucidation. Additionally, the extraction of valuable biopolymers like chitin and chitosan from spent fungal biomass, which is predominantly composed of cell walls, represents an underexplored opportunity for circular bioeconomy. Questions persist regarding the effective extraction of these biopolymers from the cell wall and their repurposing in valorization processes. This review aims to address these issues and promote further research on understanding the cell walls in filamentous fungi to optimize their biotechnological use.</p><p><i>• The highly complex cell walls of filamentous fungi are important for biotechnology.</i></p><p><i>• Cell wall mutants show promising potential to improve industrial enzyme secretion.</i></p><p><i>• Recent studies revealed enhanced avenues for chitin/chitosan from fungal biomass.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13512-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of metagenomic analysis on the discovery of novel endolysins","authors":"Nikolaos Georgakis,&nbsp;Georgios E. Premetis,&nbsp;Panagiota Pantiora,&nbsp;Christina Varotsou,&nbsp;Charoutioun S. Bodourian,&nbsp;Nikolaos E. Labrou","doi":"10.1007/s00253-025-13513-2","DOIUrl":"10.1007/s00253-025-13513-2","url":null,"abstract":"<p>Metagenomics has revolutionized enzyme discovery by enabling the study of genetic material directly from environmental samples, bypassing the need for microbial cultivation. This approach is particularly effective for identifying novel endolysins, phage-derived enzymes with antibacterial properties suited for therapeutic and industrial applications. Diverse ecosystems, such as biofilms, human microbiome, hot springs, and geothermal areas, serve as rich reservoirs for endolysins with traits like thermostability, broad-spectrum activity, specificity and resistance to harsh conditions. Functional metagenomics, complemented by bioinformatics, enables the discovery and annotation of previously uncharacterized endolysins. Examples of endolysins discovered from metagenomics analysis are discussed. Despite the challenges of analyzing complex microbial ecosystems and isolating target genes, metagenomics holds immense potential for uncovering innovative endolysins, paving the way for developing new biotechnological applications.</p><p><i>• Endolysins offer antibacterial potential for therapeutic and industrial use.</i></p><p><i>• Metagenomics enables discovery of novel endolysins from diverse ecosystems.</i></p><p><i>• Advances in tools and methods have accelerated novel endolysins discovery.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13513-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering factors influencing planktonic and sedimentary microbial assembly processes in Midwest salinity lakes","authors":"Jincheng Hu,&nbsp;Tianyi Chen,&nbsp;Chun-Ang Lian,&nbsp;Li Wang,&nbsp;Weiqin Zhuang,&nbsp;Ke Yu","doi":"10.1007/s00253-025-13498-y","DOIUrl":"10.1007/s00253-025-13498-y","url":null,"abstract":"<p>The salt lake ecosystem, characterized by extreme environmental gradients, harbors microbes that uniquely adapt to high salt stress through natural selection. However, how abiotic and biotic factors shape the microbial community assembly in Yuncheng Salt Lakes remains unclear. Here, we investigated the assembly processes and meta co-occurrence patterns of microbiota in both water and sediment sampled from 14 distinct wide range of salinity lakes in the Shanxi Yuncheng area, Midwest of China, using 16S rRNA and 18S rRNA gene sequencing technology combined with multivariate ecological and statistical methods. Habitat differentiation led to the differences in microbial diversity, co-occurrence patterns, and community assembly between sedimentary and planktonic communities. Sedimentary prokaryotes were more shaped by deterministic processes than planktonic bacterial communities. Salinity was a major abiotic factor influencing the balance between stochastic and deterministic processes in both sediment and water. Enhanced microbial interactions within sediments exhibited a more prominent impact in shaping community assembly, as indicated by the stronger association between network-inferred species and prokaryotic βNTI. Moreover, we revealed significant differences in how core species concerning βNTI responded to biotic and abiotic factors. Our findings elucidated the ecological process underlying microbial communities in Yuncheng Salt Lakes and shed light on the mechanism of microorganisms to maintain community complexity and diversity in the extreme environment.</p><p><i>• Sedimentary prokaryotes were more shaped by deterministic processes than planktonic prokaryotic communities.</i></p><p><i>• Salinity was a major factor influencing the balance between stochastic and deterministic process.</i></p><p><i>• Inter-domain and intra-domain symbiotic interactions within sedimentary communities represent key biotic factors influencing their community assembly.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13498-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substrate utilization and secondary metabolite biosynthesis in the phylum Planctomycetota","authors":"Madeleine Kündgen,&nbsp;Christian Jogler,&nbsp;Nicolai Kallscheuer","doi":"10.1007/s00253-025-13514-1","DOIUrl":"10.1007/s00253-025-13514-1","url":null,"abstract":"<p>The phylum <i>Planctomycetota</i> is changing our understanding of bacterial metabolism, driving critical biogeochemical processes through the transformation of complex polymeric substrates into valuable bioactive compounds. Sophisticated methods for cultivation, genome sequencing and genetic strain engineering developed in the last two decades have stimulated detailed studies on cell propagation, metabolic capabilities and potential applications of phylum members beyond the mere isolation and characterization of novel taxa. This review synthesizes recent advances in understanding the <i>Planctomycetota</i> physiology with a focus on the degradation of phototroph-derived polysaccharides, anaerobic ammonium oxidation (anammox) and biosynthesis of secondary metabolites. New data especially collected over the last 5 years justifies more intensive research of the yet uncharacterized pathways of substrate uptake and utilization, as well as genome mining-assisted bioprospection to exploit the phylum's chemical repertoire.</p><p>• <i>Planctomycetes can degrade high-molecular-weight sugars produced by algae</i></p><p>• <i>Anaerobic ammonium oxidation (anammox) is used in technical applications</i></p><p>• <i>The first secondary metabolites were discovered in the last 5 years</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13514-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a novel alginate lyase Alg0392 with organic solvent-tolerance from Alteromonas sp. A1-6","authors":"Tong Liang,&nbsp;Jing Chen,&nbsp;Jing Li,&nbsp;Ming-li Dong,&nbsp;Zhenggang Han,&nbsp;Feng-juan Shan,&nbsp;Xue-wang Gao,&nbsp;Da-zhong Yan","doi":"10.1007/s00253-025-13506-1","DOIUrl":"10.1007/s00253-025-13506-1","url":null,"abstract":"<p>Enzymatic depolymerization of seaweed polysaccharides aroused great interest in the production of functional oligosaccharides and fermentable sugars. Alginate lyase Alg0392, a potential novel member of the polysaccharide lyase PL17 family, was cloned from <i>Alteromonas</i> sp. A1-6. The enzymatic properties, kinetic parameters, and hydrolytic products of Alg0392 were systematically characterized. Especially, the recombinant enzyme Alg0392 showed excellent tolerance to organic reagents. When treated with 5 mmol/L of TritonX-100 or 20%(v/v) of methanol, its relative enzyme activity could be maintained at more than 70%. The recombinant enzyme has a substrate preference for poly (β-D-mannuronic acid). The products of alginate hydrolysis catalyzed by Alg0392 are mainly monosaccharides, disaccharides, and trisaccharides. The products generated by the degradation of polymannuronic acid (polyM) are mainly monosaccharides. So Alg0392 is a polymannuronate cleaving enzyme. It has excellent organic solvent-tolerance and possesses both endo- and exo-glycosidase activities towards alginate. These unique properties make the recombinant enzyme Alg0392 more advantageous for the future industrial production of biofuels and the preparation of alginate oligosaccharides.</p><p>•<i> Alg0392 is a bifunctional alginate lyase with exolytic and endolytic cleavage activity.</i></p><p>•<i> Alg0392 exhibits excellent organic solvent tolerance.</i></p><p>•<i> The enzymatic hydrolysates of Alg0392 exhibit antioxidant activity.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13506-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}