{"title":"乳清乳酸菌与木林komagataeibacillus xylinus共培养制备细菌纤维素的研究","authors":"Justyna Płoska, Monika Garbowska, Iwona Ścibisz, Lidia Stasiak-Różańska","doi":"10.1007/s00253-025-13582-3","DOIUrl":null,"url":null,"abstract":"<p>The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing <i>K. xylinus</i> with selected strains of lactic acid bacteria and comparing the properties of this biopolymer with BC obtained in <i>K. xylinus</i> monoculture. The <i>K. xylinus</i> + <i>Lb. acidophilus</i> co-culture yielded 2.19 g·L<sup>−1</sup> of BC, which was 125% more than the <i>K. xylinus</i> monoculture. Additionally, <i>K. xylinus</i> in co-culture with <i>Lb. acidophilus</i> increased the degradation temperature of BC to 361 °C compared to 303 °C for BC obtained in monoculture. The BC obtained in the co-cultures showed better mechanical properties. BC obtained in co-culture with <i>Lb. delbrueckii</i> showed more than twice the Young’s modulus than BC from monoculture. Moreover, strain at break BC from co-culture with <i>Lb. acidophilus</i> and stress at break BC from co-culture with <i>Lb. helveticus</i> were 72% and 54% higher, respectively, than BC obtained from monoculture <i>K. xylinus.</i> In this study, it was shown that conducting acetic-lactic co-cultures increased the efficiency of BC biosynthesis and improved its properties. Moreover, this study has shown that acid whey is a sufficient and complete substrate for obtaining BC. Results presented in this paper indicate new possibilities for the management of this side product.</p><p>• <i>The K. xylinus + Lb. acidophilus co-culture produced 125% more cellulose than the monoculture.</i></p><p>• <i>High lactic acid content and low pH of acid whey enhance cellulose biosynthesis.</i></p><p>• <i>Acetic acid-lactic acid co-cultures improved the mechanical properties of cellulose.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13582-3.pdf","citationCount":"0","resultStr":"{\"title\":\"Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey\",\"authors\":\"Justyna Płoska, Monika Garbowska, Iwona Ścibisz, Lidia Stasiak-Różańska\",\"doi\":\"10.1007/s00253-025-13582-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing <i>K. xylinus</i> with selected strains of lactic acid bacteria and comparing the properties of this biopolymer with BC obtained in <i>K. xylinus</i> monoculture. The <i>K. xylinus</i> + <i>Lb. acidophilus</i> co-culture yielded 2.19 g·L<sup>−1</sup> of BC, which was 125% more than the <i>K. xylinus</i> monoculture. Additionally, <i>K. xylinus</i> in co-culture with <i>Lb. acidophilus</i> increased the degradation temperature of BC to 361 °C compared to 303 °C for BC obtained in monoculture. The BC obtained in the co-cultures showed better mechanical properties. BC obtained in co-culture with <i>Lb. delbrueckii</i> showed more than twice the Young’s modulus than BC from monoculture. Moreover, strain at break BC from co-culture with <i>Lb. acidophilus</i> and stress at break BC from co-culture with <i>Lb. helveticus</i> were 72% and 54% higher, respectively, than BC obtained from monoculture <i>K. xylinus.</i> In this study, it was shown that conducting acetic-lactic co-cultures increased the efficiency of BC biosynthesis and improved its properties. Moreover, this study has shown that acid whey is a sufficient and complete substrate for obtaining BC. Results presented in this paper indicate new possibilities for the management of this side product.</p><p>• <i>The K. xylinus + Lb. acidophilus co-culture produced 125% more cellulose than the monoculture.</i></p><p>• <i>High lactic acid content and low pH of acid whey enhance cellulose biosynthesis.</i></p><p>• <i>Acetic acid-lactic acid co-cultures improved the mechanical properties of cellulose.</i></p>\",\"PeriodicalId\":8342,\"journal\":{\"name\":\"Applied Microbiology and Biotechnology\",\"volume\":\"109 1\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s00253-025-13582-3.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Microbiology and Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00253-025-13582-3\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Microbiology and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s00253-025-13582-3","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey
The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing K. xylinus with selected strains of lactic acid bacteria and comparing the properties of this biopolymer with BC obtained in K. xylinus monoculture. The K. xylinus + Lb. acidophilus co-culture yielded 2.19 g·L−1 of BC, which was 125% more than the K. xylinus monoculture. Additionally, K. xylinus in co-culture with Lb. acidophilus increased the degradation temperature of BC to 361 °C compared to 303 °C for BC obtained in monoculture. The BC obtained in the co-cultures showed better mechanical properties. BC obtained in co-culture with Lb. delbrueckii showed more than twice the Young’s modulus than BC from monoculture. Moreover, strain at break BC from co-culture with Lb. acidophilus and stress at break BC from co-culture with Lb. helveticus were 72% and 54% higher, respectively, than BC obtained from monoculture K. xylinus. In this study, it was shown that conducting acetic-lactic co-cultures increased the efficiency of BC biosynthesis and improved its properties. Moreover, this study has shown that acid whey is a sufficient and complete substrate for obtaining BC. Results presented in this paper indicate new possibilities for the management of this side product.
• The K. xylinus + Lb. acidophilus co-culture produced 125% more cellulose than the monoculture.
• High lactic acid content and low pH of acid whey enhance cellulose biosynthesis.
• Acetic acid-lactic acid co-cultures improved the mechanical properties of cellulose.
期刊介绍：
Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.
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