Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey

IF 4.3 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Microbiology and Biotechnology Pub Date : 2025-08-21 DOI:10.1007/s00253-025-13582-3

Justyna Płoska, Monika Garbowska, Iwona Ścibisz, Lidia Stasiak-Różańska

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引用次数: 0

Abstract

The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing K. xylinus with selected strains of lactic acid bacteria and comparing the properties of this biopolymer with BC obtained in K. xylinus monoculture. The K. xylinus + Lb. acidophilus co-culture yielded 2.19 g·L⁻¹ of BC, which was 125% more than the K. xylinus monoculture. Additionally, K. xylinus in co-culture with Lb. acidophilus increased the degradation temperature of BC to 361 °C compared to 303 °C for BC obtained in monoculture. The BC obtained in the co-cultures showed better mechanical properties. BC obtained in co-culture with Lb. delbrueckii showed more than twice the Young’s modulus than BC from monoculture. Moreover, strain at break BC from co-culture with Lb. acidophilus and stress at break BC from co-culture with Lb. helveticus were 72% and 54% higher, respectively, than BC obtained from monoculture K. xylinus. In this study, it was shown that conducting acetic-lactic co-cultures increased the efficiency of BC biosynthesis and improved its properties. Moreover, this study has shown that acid whey is a sufficient and complete substrate for obtaining BC. Results presented in this paper indicate new possibilities for the management of this side product.

• The K. xylinus + Lb. acidophilus co-culture produced 125% more cellulose than the monoculture.

• High lactic acid content and low pH of acid whey enhance cellulose biosynthesis.

• Acetic acid-lactic acid co-cultures improved the mechanical properties of cellulose.

查看原文本刊更多论文

乳清乳酸菌与木林komagataeibacillus xylinus共培养制备细菌纤维素的研究

酸乳清作为培养基的使用是一种创新的方法，细菌纤维素（BC）的生物合成在乙酸细菌与乳酸菌共培养。本研究的目的是评价木曲菌与选定的乳酸菌菌株共培养在酸性乳清中获得BC的可能性，并将这种生物聚合物与木曲菌单一培养获得的BC的特性进行比较。木霉+ Lb。嗜酸菌共培养的BC含量为2.19 g·L−1，比木林松单培养的BC含量高125%。此外，与嗜酸Lb. ophilus共培养时，木林氏K. xylinus将BC的降解温度提高到361°C，而单独培养时BC的降解温度为303°C。共培养得到的BC表现出较好的力学性能。与lb.delbrueckii共培养获得的BC的杨氏模量比单一培养的BC的杨氏模量高出一倍以上。此外，与嗜酸Lb.共培养的菌株和与helveticus Lb.共培养的菌株分别比单一培养的木霉菌株高72%和54%。本研究表明，乙酸-乳酸共培养提高了BC的生物合成效率，并改善了其性能。此外，本研究还表明，酸性乳清是获得BC的充分和完整的底物。本文提出的结果为管理这一副产物提供了新的可能性。•木霉+ Lb；嗜酸菌共培养比单一培养多产生125%的纤维素。•高乳酸含量和酸性乳清的低pH值促进纤维素的生物合成。醋酸-乳酸共培养提高了纤维素的机械性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物

CiteScore

10.00

自引率

4.00%

发文量

535

审稿时长

2 months

期刊介绍： Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.