Archives of Physiology and Biochemistry最新文献

{"title":"Evaluation of leptin and leptin receptor gene polymorphisms in bipolar disorder: focus on depressive episodes with atypical features.","authors":"Hasan Mervan Aytac, Yasemin Oyaci, Eren Aytac, Mustafa Pehlivan, Furkan Bahadir Alptekin, Sacide Pehlivan","doi":"10.1080/13813455.2025.2507751","DOIUrl":"https://doi.org/10.1080/13813455.2025.2507751","url":null,"abstract":"<p><p><b>Objective</b>: Our study aims to examine the effect of leptin (<i>LEP</i>) (-2548 G > A) (rs7799039) and leptin receptor (<i>LEPR</i>) (668 A > G) (rs1137101) gene polymorphisms on the etiopathogenesis of bipolar disorder (BD) by comparing them with a control group, as well as their association with depressive episodes featuring atypical symptoms.</p><p><p><b>Methods</b>: The study included a sample of 103 individuals with BD and 103 healthy volunteers. Gene polymorphisms were identified from DNA samples through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).</p><p><p><b>Results</b>: The distribution of <i>LEPR</i> genotypes was significantly different between BD patients and healthy controls. Specifically, the homozygous <i>LEPR</i> GG genotype was more common in the control group than in BD patients. Additionally, genotype frequency distributions among BD patients varied significantly based on the presence of atypical depressive episodes. The <i>LEPR</i> AA and <i>LEP</i> GG genotypes were significantly more common among patients with a history of atypical depression. Logistic regression showed that the <i>LEP</i> polymorphism and Hamilton Depression Rating Scale (HAM-D) score predict atypical depression in BD.</p><p><p><b>Conclusion</b>: In summary, while the <i>LEPR</i> polymorphism is linked to BD, the <i>LEP</i> polymorphism and symptom severity appear to predict atypical depressive episodes.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant and anti-inflammatory effects of gallic acid and/or cerium oxide nanoparticles synthesized by gamma-irradiation ameliorate cisplatin-induced hepatic injury.","authors":"Mostafa Saif-Elnasr, Alshimaa M Elmalawany, Assmaa Fathi Abdel-Khalek","doi":"10.1080/13813455.2025.2507760","DOIUrl":"https://doi.org/10.1080/13813455.2025.2507760","url":null,"abstract":"<p><strong>Context: </strong>Cisplatin is a widely utilised chemotherapeutic agent for cancer therapy, but various systemic toxicities limit its effectiveness.</p><p><strong>Objective: </strong>This study aimed to assess the hepatoprotective properties expressed by gallic acid (GA) and gamma-irradiation synthesized cerium oxide nanoparticles (CONPs) in response to hepatic damage produced by cisplatin in albino rats.</p><p><strong>Materials and methods: </strong>Serum AST and ALT activities and levels of MDA, TAC, NF‑kB, TNF‑α, and TGF‑β were determined in hepatic tissue, along with histopathological examination.</p><p><strong>Results: </strong>The executive impact of cisplatin led to a notable rise in the serum activity of hepatic enzymes AST and ALT. Conversely, in the groups receiving treatment with either or both GA and CONPs, the liver function enzymes exhibited a decline in their activity. In addition, in the hepatotoxicity models, the levels of hepatic MDA were significantly increased, accompanied by a reduction of hepatic TAC. While administration of GA, CONPs or their combination to cisplatin-injected rats resulted in a noteworthy reduction in MDA level. Conversely, the hepatic TAC level increased compared to the group that received cisplatin. The hepatic tissue architecture in rats exposed to cisplatin was found to undergo significant alterations. Furthermore, the cisplatin induced overexpression of NF-kB, TNF-α, and TGF-β. The hepatic histopathological changes observed in rats induced with cisplatin were significantly attenuated after pre-treatment with GA, CONPs, and their combination. GA, CONPs, and their co-administration resulted in reducing the levels of NF-κB, TNF-α and TGF-β compared to the group received cisplatin.</p><p><strong>Discussion and conclusion: </strong>In summary, GA and CONPs synthesized by gamma-irradiation resulted in a noteworthy reduction of liver damage caused by cisplatin exposure. Their potent antioxidant and immunoprotective properties were cited as the cause of this phenomenon.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of a multi-species probiotic supplementation on clinical symptoms and biochemical factors in patients with irritable bowel syndrome: a randomised controlled trial.","authors":"Shirin Hajiani, Maryam Tajabadi Ebrahimi, Amir Sadeghi, Nakisa Zarrabi Ahrabi, Abbas Yadegar","doi":"10.1080/13813455.2025.2507749","DOIUrl":"https://doi.org/10.1080/13813455.2025.2507749","url":null,"abstract":"<p><p><b>Background:</b> Intestinal inflammation and oxidative stress contribute to the pathophysiology of irritable bowel syndrome (IBS). This clinical trial investigated the effects of a probiotic supplementation on symptom severity and quality of life (QOL) in IBS patients.</p><p><p><b>Methods:</b> Forty-six IBS patients were randomised to receive either a multi-species probiotic or placebo for 8 weeks. Clinical symptoms were evaluated using the IBS Severity Scoring System (IBS-SSS) and IBS QOL questionnaire. Serum levels of IFN-γ, IL-1β, IL-10, IL-6, malondialdehyde (MDA), total antioxidant capacity (TAC), and nitric oxide (NO) were measured before and after the intervention.</p><p><p><b>Results:</b> After 8 weeks, the probiotic group showed significant improvements in QOL scores, and reductions in IBS-SSS and MDA levels compared to placebo group. TAC levels were significantly higher in probiotic group. However, no significant differences were observed in cytokine or NO levels.</p><p><p><b>Conclusions:</b> This multi-species probiotic supplement was safe and effective in reducing symptom severity and improved QOL of IBS patients.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination therapy of systemic and local metformin improves imiquimod-induced psoriasis-like lesions with type 2 diabetes: the role of AMPK/KGF/STAT3 axis.","authors":"Fatma ElSayed Hassan, Basma Emad Aboulhoda, Marwa Nagi Mehesen, Passant Mohie El Din, Hend Ahmed Abdallah, Ehab R Bendas, Laila Ahmed Rashed, Abeer Mostafa, Marwa Fathy Amer, Marwa Abdel-Rahman, Mansour A Alghamdi, Asmaa Mohammed Shams Eldeen","doi":"10.1080/13813455.2024.2407547","DOIUrl":"10.1080/13813455.2024.2407547","url":null,"abstract":"<p><strong>Context: </strong>Insulin resistance and a disturbed lipid profile are common associations with type 2 diabetes mellitus (T2DM) and different skin diseases, particularly psoriasis (PsO).</p><p><strong>Objectives: </strong>We investigated potential therapeutic mechanisms of metformin in a murine animal model of psoriasiform lesions in T2DM.</p><p><strong>Materials and methods: </strong>Forty-two rats were randomly divided into control, PsO, and type II DM (T2DM) groups. After confirmation of DM, the type II diabetic rats were allocated into T2DM+ PsO, T2DM+ PsO+ systemic metformin (S. met), T2DM+ PsO+ topical metformin (T. met)), and T2DM+ PsO + combined metformin (C. met). PsO was induced by topical imiquimod.</p><p><strong>Results: </strong>Systemic administration of the cornerstone antidiabetic drug, metformin, was able to improve insulin resistance and lipid profile. At molecular levels, both topical and systemic metformin significantly increased AMP-activated protein kinase (AMPK), and lowered keratinocyte growth factor (KGF) / \"Signal transducer and activator of transcription\" (STAT)3 protein levels, and the IL-17RA and IL-17RC gene expression.</p><p><strong>Conclusion: </strong>Although its glucose-controlling effect was not optimum, T.met gel served anti-psoriatic and anti-inflammatory effects.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"252-264"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of <i>Helicobacter pylori</i>-derived extracellular vesicles on glucose metabolism and induction of insulin resistance in HepG2 cells.","authors":"Ghazaleh Talebi, Parvaneh Saffarian, Mojdeh Hakemi-Vala, Amir Sadeghi, Abbas Yadegar","doi":"10.1080/13813455.2024.2418494","DOIUrl":"10.1080/13813455.2024.2418494","url":null,"abstract":"<p><p><i>Helicobacter pylori</i> infection has been associated with the development of insulin resistance (IR). This study aimed to examine the effect of <i>H. pylori</i>-derived extracellular vesicles (EVs) on IR induction. EVs were derived from two <i>H. pylori</i> strains, and characterised by transmission electron microscopy and dynamic light scattering. Different concentrations of insulin were added to HepG2 cells to induce IR model. HepG2 cells were exposed to various concentrations of <i>H. pylori</i>-derived EVs to assess IR development. The gene expression of <i>IRS1</i>, <i>AKT2</i>, <i>GLUT2</i>, <i>IL-6</i>, <i>SOCS3</i>, <i>c-Jun</i> and miR-140 was examined using RT-qPCR. Glucose uptake analysis revealed insulin at 5 × 10 ^-7mol/l and EVs at 50 µg/ml induced IR model in HepG2 cells. <i>H. pylori</i>-derived EVs downregulated the expression level of <i>IRS1</i>, <i>AKT2</i>, and <i>GLUT2</i>, and upregulated <i>IL-6</i>, <i>SOCS3</i>, <i>c-Jun</i>, and miR-140 expression in HepG2 cells. In conclusion, our findings propose a novel mechanism by which <i>H. pylori-</i>derived EVs could potentially induce IR.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"316-327"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of exogenous ghrelin treatment on oxidative stress, inflammation and histological parameters in a fat-fed streptozotocin rat model.","authors":"Ozlem Ergul Erkec, Zubeyir Huyut, Eda Acikgoz, Mehmet Tahir Huyut","doi":"10.1080/13813455.2024.2407551","DOIUrl":"10.1080/13813455.2024.2407551","url":null,"abstract":"<p><p>In this study, the anti-inflammatory, antioxidative, and protective effects of ghrelin were investigated in a fat-fed streptozotocin (STZ) rat model and compared with metformin, diabetes and the healthy control groups. Histopathological evaluations were performed on H&E-stained pancreas and brain sections. Biochemical parameters were investigated by enzyme-linked immunosorbent assay. Blood glucose levels were significantly decreased with ghrelin or metformin treatments than the diabetes group. STZ administration increased brain, renal and pancreatic IL-1β, TNF-α and MDA while decreasing GPX, CAT, SOD, and NGF levels. Ghrelin increased renal GPX, CAT, NGF pancreatic GPX, SOD, CAT, NGF and brain SOD, NGF while it decreased renal, pancreatic and brain IL-1β, TNF-α and MDA levels. Ghrelin reduced neuronal loss and degeneration in the cerebral cortex and hippocampus and greatly ameliorated diabetes-related damage in pancreas. In conclusion, the data suggested that ghrelin is an effective candidate as a protectant for reducing the adverse effects of diabetes.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"274-284"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/13813455.2024.2418702","DOIUrl":"10.1080/13813455.2024.2418702","url":null,"abstract":"","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"328"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artichoke leaf hydroethanolic extract reduces neuropathic pain in a rat model of chronic constriction injury via attenuating the sciatic nerve oxidative stress.","authors":"Mohammad Mehdi Haghighat Lari, Mohammad Reza Bakhoda, Mohammad Shabani, Mohsen Taghizadeh, Fereshteh Bahmani, Gholamali Hamidi, Fatemeh Aghighi, Sayyed Alireza Talaei","doi":"10.1080/13813455.2024.2406898","DOIUrl":"10.1080/13813455.2024.2406898","url":null,"abstract":"<p><p>Neuropathic pain, a nerve damage consequence, presents symptoms such as dysesthesia, hyperalgesia, and allodynia. This study aimed to evaluate the alleviating potential of artichoke leaf extract in neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in male rats. The hydroethanolic extract of artichoke leaf was administered via gavage at doses of 200, 400, and 800 mg/kg for 21 days. Behavioural tests were conducted on days 1, 4, 7, 14, and 21 post-surgeries. Only the dose of 800 mg/kg significantly reduced thermal hyperalgesia and allodynia from day 14 and mechanical allodynia from day 7, and the other doses did not affect behaviours. Biochemical analysis showed that artichoke extract decreased lipid peroxidation and restored antioxidant enzyme activities (SOD and GPx) in the sciatic nerve tissue. In conclusion, artichoke leaf extract administration diminishes neuropathic pain-related behaviours by enhancing antioxidant capacity and reducing oxidative stress in the rats' sciatic nerve.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"227-233"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway.","authors":"Peipei Zhang, Zhilong Liu, Guiqiao Ma, Junwei Wang, Jing Shao, Chaojing Ma, Liping Wang, Chanjuan Ma","doi":"10.1080/13813455.2024.2407552","DOIUrl":"10.1080/13813455.2024.2407552","url":null,"abstract":"<p><p>Diabetic kidney disease (DKD) is one of the common microvascular complications of diabetes, and there are still lack of effective treatments. Huaiqihuang (HQH) is a kind of traditional Chinese medicine mixed preparation, which is mainly made of Trametes robiniophila Murr, Fructus Lycii, and Polygonatum sibiricumhas. It has been shown to be effective in the treatment of DKD, but the specific mechanism has not been fully elucidated. Our results showed that HQH increased the protein expressions of synaptopodin, podocin, WT-1, and Bcl-2, decreased the protein expressions of Bax and cleaved-casepase-3, and activated the NF-ĸB and PI3K/AKT/mTOR pathway in MPC5 cells exposed to high-glucose (HG). Real-time PCR results showed that HQH reduced the mRNA expression of TNF-α, IL-1β, MCP-1, and IL-6. In conclusion, our results showed that HQH may attenuate podocyte injury by inhibiting MPC5 cell apoptosis induced by HG and NF-κB-mediated inflammation response through activation of the PI3K/AKT/mTOR pathway.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"285-292"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effects of <i>Myrtus communis</i> subsp. on ovariectomized diabetic rats' renal and intestinal tissues: <i>in vivo</i> and <i>in silico</i> approaches.","authors":"Onur Ertik, Beril Kadıoğlu-Yaman, Ali Şen, Göksel Şener, Refiye Yanardag","doi":"10.1080/13813455.2024.2406895","DOIUrl":"10.1080/13813455.2024.2406895","url":null,"abstract":"<p><strong>Introduction: </strong>Postmenopausal diabetes is a condition that affects millions of women and their quality of life. Also, kidney and small intestine tissues are damaged due to diabetes. The present study aimed to examine the protective effects of an extract prepared from <i>Myrtus communis</i> leaves on kidney and small intestine tissues against experimentally created postmenopausal diabetes.</p><p><strong>Methods: </strong>For this purpose, experimental rats were randomly divided into six groups (Control; ovariectomy:OVX, diabetic:D, ovariectomy + diabetic:OVX + D, ovariectomy + diabetic + oestrogen:OVX + D+E2, ovariectomy + diabetic + MC: OVX + D+MC) and kidney and small intestine tissues were taken after the experimental procedure.</p><p><strong>Results: </strong>Evaluations of biochemical parameters (glutathione and glutathione-related enzymes, antioxidant enzymes, etc.) showed that MC had a protective effect on kidney and small intestine tissues in diabetes and ovariectomy groups.</p><p><strong>Conclusion: </strong>It can be suggested that MC extract has a protective effect on small intestine and kidney tissues in postmenopausal diabetes and may be a good herbal source for this purpose.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"210-226"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}