Annals of Clinical and Laboratory Research最新文献

{"title":"Imprecision Evaluation of Self-Monitoring of Blood Cholesterol (SMBC)Handheld Point of Care Testing Devices: Elemark⢠and Cardiochek PA","authors":"J. Bolodeoku., S. Pinkney","doi":"10.21767/2386-5180.100290","DOIUrl":"https://doi.org/10.21767/2386-5180.100290","url":null,"abstract":"The aim of this study was to determine the imprecision of two cholesterol point of care testing devices, the very established CardioChek PA and recently the Elemark™ which is a mobile blood testing device capable of testing multiple parameters for the management of hypercholesterolaemia undergoing certification. The imprecision study of the Elemark™ and CardioChek PA were performed using blood donations from 3 healthy normal lipidemic subjects. There were 10 repetitions for the within run imprecision and the number of measurement days was 6 for between day imprecision. This study showed comparable within run and between day imprecision between the CardioChek PA and the Elemark™. Based on the imprecision guidelines from the NCEP and CRMLN both devices showed reasonable within run imprecision for their total cholesterol and HDLcholesterol estimations but not the LDL-cholesterol calculations. The between day imprecision for both devices was reasonably consistent with the published within person biological variability. With more accuracy and imprecision studies being carried out in understanding these cholesterol testing point of care devices, their value, or lack of, in the lipid monitoring would become more apparent.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"1245 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83777432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Pyrrolidine Dithiocarbamate to Liver Injury in a Sepsis Model with Cecum Ligation and Perforation â An Animal Study","authors":"Abdülcelil Gezmiş, Bedih Balkan, A. Yektaş","doi":"10.21767/2386-5180.100294","DOIUrl":"https://doi.org/10.21767/2386-5180.100294","url":null,"abstract":"Introduction: Pyrrolidine dithiocarbamate is a low molecular thiol antioxidant and is a strong inhibitor of nuclear factor kappa B activation. Recent animal studies have shown a delaying effect of intraperitoneal sepsis on healing after colon anastomosis. Our aim in this study is to use pyrrolidine dithiocarbamate in sepsis treatment to research the place of nuclear factor kappa B inhibition in preventing tissue injury and organ function disorders and the effect on organ failure caused by clinical worsening of sepsis. Materials and methods: Our study was completed on a total of 28 rats in Istanbul University Faculty of Medicine Hospital. In our study subjects were grouped as control (n=4) liver subjects, “Group A” (n=4) (sham group), “Group B” (n=8) (Sepsis group) (laparotomy and cecal mobilization), “Group C” (n=8) Pyrrolidine dithiocarbamate subjects and “Group D” (n=8) sepsis+ Pyrrolidine dithiocarbamate subjects. Apart from the sham group (control group) rats had the polymicrobial sepsis model described in the literature of cecum ligation and perforation used to induce sepsis. Group A (sham group, n=8) had mini laparatomy performed after anaesthesia and then the abdomen was closed without cecum ligation and perforation. Group B (sepsis group, n=8) had the cecum isolated with mini laparotomy, sepsis was induced with the cecum ligation and perforation method and then the abdomen was closed. Group C (Pyrrolidine dithiocarbamate, n=8) had 100 mg/kg/day Pyrrolidine dithiocarbamate administered by gavage 15 minutes before and 12 hours after mini laparotomy procedure. Group D (sepsis+Pyrrolidine dithiocarbamate, n=8) had pyrrolidine dithiocarbamate administed by gavage 15 minutes before and 12 hours after cecum ligation and perforation to induce sepsis. Results: When mortality in the groups is assessed, as there was no procedure performed on the sham group (performed cecum ligation and perforation or antioxidant administered) no rat died and all 8 rats were sacrificed for histopathological and immunohistochemical assessment. In the Group B of the 7 rats that died, 5 died from 24-36 hours, and 2 were exitus before 48 hours. In Group D (sepsis+ Pyrrolidine dithiocarbamate) 2 were exitus in the first 48 hours with the other 2 exitus on the 5th day and 3 rats were alive on the 10th day. In Group C (sham +Pyrrolidine dithiocarbamate) 1 rat was exitus in the first 72 hours, while the remaining 6 were still alive on the 10th day. The livers of the cases were investigated for morphology, p65 and inductible nitic oxide synthase activity after hematoxylin-eosin and immunohistochemical staining. The results were statistically assessed. Conclusion: In these results, pyrrolidine dithiocarbamate appears to be an appropriate medication with potential efficacy for sepsis and septic shock treatment. As the results of clinical studies of antioxidants used for sepsis treatment are still controversial, there is a need for broader and longer duration studies.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84542519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of Serum Ascorbic Acid (Vitamin C) In the Age Related (Senile) Cataract: a Case Control Study","authors":"Saseekala Angirekula, L. Atti, Srihari Atti","doi":"10.21767/2386-5180.1000217","DOIUrl":"https://doi.org/10.21767/2386-5180.1000217","url":null,"abstract":"Aim: The aim was to estimate the serum Ascorbic Acid (Vitamin C) level, in the development of Age Related (Senile) Cataract, a common cause of curable blindness globally, especially in India. Materials and methods: This was a case control study in the age group of 45-70 years with 100 patients of age related cataract and 100 age matched normal patients as a control group with estimation of the level of vitamin C in the serum of the study group and the control group. Results: Serum ascorbic acid values were 0.54 ± 0.23 mg/dL (30.68 ± 13.07 umol/L) in senile cataract and 0.83 ± 0.35 mg/dl (47.16 ± 19.89 umol/L) in controls with a statistical difference between the cases and controls showing a significant decrease in the level of vitamin C in the serum of age related cataract patients compared with the control group. Conclusion: The present study with the low levels of Vitamin C in serum of age related cataract than in controls shows that the reduced antioxidant status defence mechanism due to the reduced serum Ascorbic acid (Vitamin C) may play a role in the development of the senile cataract.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74144856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral-Facial-Digital Syndrome Type II (Mohr Syndrome) in Palestine","authors":"A. Abuhamda, A. Elsous","doi":"10.21767/2386-5180.100259","DOIUrl":"https://doi.org/10.21767/2386-5180.100259","url":null,"abstract":"Oral-facial-digital syndrome (OFDS) is a group of disorders characterized by oral cavity malformation, facial deformities and digits anomalies. There are at least 13 forms of OFDS, in which most of them have brain anomalies and different degrees of mental retardation. We have reported two cases with quite different dysmorphic features. The first case was a female and had small wide-spaced eyes, wide nose bridge, middle line cleft of upper lip, cleft hard palate, microglossia, micrognathia, polydactyly and syndactyly of the upper and lower limbs. Moreover, she had congenital hypotonia. Brain and abdominal ultrasound studies were normal. The baby's mother had a history of losing her first pregnancy resulted from intrauterine fetal death who was presented with similar dysmorphic features. The second case was a 4-year-old male who had dysmorphic features; Antimongoloid eye slant, prominent ears, broad nose bridge, high arched palate, cleft soft palate, lingual nodule, preaxial and posaxial polydactyly with syndactyly in both hands, bilateral bifid thumbs and bilateral clinodactyly. Feet had polydactyly with syndactyly, and bifid big toe shape. The first time, that two cases with characteristic features of oral facial digital syndrome type II to be diagnosed in two Palestinian siblings of different family with consanguineous marriage.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75864637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malaria Rapid Diagnostic Test (RDTs)","authors":"E. Obeagu, U. Chijioke, Ifeoma Stella Ekelozie","doi":"10.21767/2386-5180.100275","DOIUrl":"https://doi.org/10.21767/2386-5180.100275","url":null,"abstract":"Malaria is a disease of the tropics and sub-tropics which is transmitted by infected female Anopheles mosquito. Malaria is one of the major infections challenging this part of the world. This leads to loss of lives every year especially children and mothers. The use of laboratory methods has become necessary because health workers cannot identify malaria cases reliably using clinical signs and symptoms alone. Conventionally, light microscopy of a blood smear is the reference gold standard for the detection of malaria parasites and the established method for the laboratory confirmation of malaria. It can detect malaria parasites even at low densities of 5-10 parasites/μl of blood when used by skilled and careful scientist. However, malaria diagnosis based on microscopy is labour intensive and time-consuming. Rapid diagnostic tests (RDTs) are recommended by WHO to enhance diagnosis and management of case prevention of complications of declared treatment, prolonging survival and monitoring of treatment especially in children. More research should be done in RDTs for higher sensitivity and specificity. Health workers especially medical laboratory scientist and clinicians should not neglect microscopy which is the gold standard for the diagnosis of malaria. Life is precious and should be carefully handled.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81503749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time for a U-Turn on Understanding the Major Cause of Universal HumanHypovitaminosis D","authors":"Abbas Tavakolian Arjm, S. Jafarian","doi":"10.21767/2386-5180.100238","DOIUrl":"https://doi.org/10.21767/2386-5180.100238","url":null,"abstract":"Vitamin D deficiency has turned into a rather concerning health issue around the globe, touching at least 40- 50% of general population. The old and stereotyped view with regard to the problem is that, the current hypovitaminosis D results from inadequate vitamin D in human diet and lack of enough sunlight exposure. And even more amusing is that the vast majority of recent epidemic human health problems are being somehow regarded as the direct outcome of this particular micronutrient deficiency. Our major aim in this brief overview, which will be followed by an opinion, is to cast a faint beam of light onto a narrow road towards a more sensible understanding of this utterly bizarre and poorly explained human health dilemma. We would like to raise the impression that the current vitamin D deficiency state is not merely a simple dietary inadequacy, but an immediate outcome of metabolic syndrome and its closely related medical entity, the nonalcoholic fatty liver disease (NAFLD). We would like to put forward the idea of vitamin D malabsorption and impaired 25-hydroxylation of vitamin D precursors in subjects affected by NAFLD, and to open a wide field of research in front of dedicated and loyal scientists.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84513440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serocarriage of Ureaplasma Urealyticum in Male of Various Age Ranges and Occupation in Nnewi, Nnewi North Local Government Area, Anambra State, Nigeria","authors":"Ochiabuto Omtb, Obeagu Ei","doi":"10.21767/2386-5180.1000219","DOIUrl":"https://doi.org/10.21767/2386-5180.1000219","url":null,"abstract":"Introduction: Awareness about Ureaplasma urealyticum is not too poor in this part of the country especially among male populace. Male infertility is increasing in this part of the country and has been proved to be up to 30% cause of infertility among couples. Lack of awareness on the causes of increase of prostatitis and other related diseases has paved a way to higher incidence of this infection. Due to the fact that this infection is mostly assymptomic, it is therefore recommended that it should be tested at least once a year. Aim: A study to determine the serocarriage of Ureaplasma urealyticum in male of various age ranges and profession in Nnewi was conducted in a total of 93 subjects. Method: Ureaplasma urealyticum antigen was analysed using ELISA method with a commensally prepared kit (UUAB kit).Using internal built control. All method was as instructed in the user’s manual. Result: Results revealed that out of the 93 male in Nnewi (single and married) that were sampled 3(3.2%) showed a positive result to Ureaplasma urealyticum, while 90(96.8%) blood sample showed a negative result to Ureaplasma urealyticum. The highest prevalence of subject was within the age range of (18-24, 25-29 and 42-47) with a percentage of 33.3% respectively, there were no marked statistical differences. There were about seven (7) professions found within the area of study but the highest prevalence were traders with a percentage of 100%, there was no marked statistical difference. The highest prevalence of subject within the marital status are those married, with a percentage of 66.7% and there was no marked statistical difference. The subject with the number of children within the range of 2 and none were the highest serocarriage of this organism with a percentage of 66.7% and 1.1% respectively and there was a marked statistical difference of 0.02. Six states were found within the area of study but the highest prevalence were Enugu and Anambra with the percentage of 66.7% and 33.3% with no statistical differences. Conclusion: Serocarriage of Ureaplasma urealyticum can be said to be of low incidence probably because of the area of study and indiscriminate use of antibiotics in the study area.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84864163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection of Doxorubicin-Induced Biochemical Injury in the Rat Bone Marrow by a Dietary Bioflavonoid Naringin","authors":"Ganesh Ch, R. Jagetia, Hmingthazuali Vl","doi":"10.21767/2386-5180.1000224","DOIUrl":"https://doi.org/10.21767/2386-5180.1000224","url":null,"abstract":"Doxorubicin is a secondary metabolite isolated, from Streptomyces peucetius and has been used as a standard chemotherapeutic agent alone or in conjunction with other chemotherapeutic drugs to treat different types of malignancies. The use of doxorubicin associated with adverse toxic side effect on bone marrow heart, kidneys and liver in patients receiving its therapy Therefore, any agent that can reduce the toxicity of doxorubicin will be useful in clinical conditions. Naringin a dietary flavonoid synthesized by different citrus fruits has been investigated for its protective effect against the doxorubicin-induced biochemical stress in albino rat bone marrow administered with 2 mg/kg body weight of naringin or 5 mg/kg body weight of doxorubicin either alone or in combination with each other. The bone marrow was aspirated at ½, 1 and 2 h post-doxorubicin treatment and activities of glutathione-s-transferase, catalase and superoxide dismutase, and glutathione concentration and lipid peroxidation were measured. Administration of doxorubicin alone caused a time dependent but significant reduction in the activities of catalase and superoxide dismutase and glutathione concentration followed by increased lipid peroxidation at ½, 1 and 2 h post-doxorubicin treatment. The treatment of rats with naringin before doxorubicin administration significantly raised the activities of catalase and glutathione concentration followed by reduction in the lipid peroxidation in the rat bone marrow. Our study shows that naringin attenuated the doxorubicin-induced biochemical stress and may be a useful agent in reducing the toxicity of doxorubicin in patients.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82382841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Frequency of Chromosomal Abnormalities in Individuals with Susceptibility to Abortion and/or Infertility by Cytogenetics Method","authors":"Lida Karimi Behbahany, F. Keshavarzi, Gholamreza Homayounpour","doi":"10.21767/2386-5180.100258","DOIUrl":"https://doi.org/10.21767/2386-5180.100258","url":null,"abstract":"Background and Aim: Abnormality karyotype of couples can produce susceptibility to infertility or abortion. Therefore, the first step to study these couples should be determining karyotype by cytogenetic method. In this study assay the prevalence of abnormalities chromosomal these couples from western provinces of Iran. Method: A cytogenetic study was performed on 200 individuals (124 women (28.1 ± 5) and 76 males (30.7 ± 5) with infertility and/or abortion with age average 30.81 ± 5 using lymphocyte culture and high-resolution G-banding method. Results: Normal karyotype and various types of chromosomal abnormalities were observed in 138 (69%) and 62 (31%) individuals, respectively. Also, the frequency of translocations, insertions, deletions, inversions and duplications were 40.32% (25), 22.6% (14), 17.74% (11), 9.7% (6) and 9.7% (6) respectively, that the highest frequency was related to changes in transposition or chromosomal displacement with 25 cases (40.3%) In addition, the types of chromosomal abnormalities involving translocations, insertions, deletions, inversions and duplications were detected in 15, 9, 6, 3 and 2 persons, respectively, from 35 patients with RLP and 10, 5, 5, 3 and 4 persons, respectively, of 27 patients with infertility. Conclusion: This issue can also play a role in the planning of pregnant mother’s health.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76534016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Series: Total Elbow Arthroplasty for Comminuted, Distal Humerus Fractures","authors":"Y. Kang, N. Yeo, Tjiauw Tjoen Denny Lie","doi":"10.21767/2386-5180.100274","DOIUrl":"https://doi.org/10.21767/2386-5180.100274","url":null,"abstract":"Introduction: Total elbow arthroplasty (TEA) is an established treatment for late-stage arthritis of the elbow. In comminuted distal humerus fractures, there is a role in performing total elbow arthroplasty to aid in the restoration of function and range of motion. This is a case series describing TEA in 3 non-rheumatoid patients who sustained comminuted distal humerus fractures. Methods: Three elderly non-rheumatoid patients who sustained comminuted distal humerus fractures underwent total elbow arthroplasty using Zimmer® Coonrad/morrey prosthesis. Demographic data was retrospectively collected and outcomes were measured using the mayo elbow performance scores. Results: Post-operatively, the patients demonstrated improvement in mayo elbow performance scores and were able to return to functional activities in their daily lives. Conclusion: TEA is a viable option for non-rheumatoid elderly patients who sustain comminuted distal humerus fractures due to a low energy fall, with satisfactory outcome measures.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91412331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}