IF 2.6 4区医学

Apmis Pub Date : 2026-06-08 DOI: 10.1111/apm.70230

Francesco Vasuri, Alessandra Boccaccino, Stefano Tamberi, Luca Saragoni

{"title":"Predictive Immunohistochemistry in Cholangiocarcinoma: Current Clinical Utility, Practical Limitations, and Emerging Directions","authors":"Francesco Vasuri, Alessandra Boccaccino, Stefano Tamberi, Luca Saragoni","doi":"10.1111/apm.70230","DOIUrl":"10.1111/apm.70230","url":null,"abstract":"Cholangiocarcinoma (CCA) remains a difficult-to-treat biliary malignancy in which therapeutic stratification increasingly depends on predictive biomarkers. Although next-generation sequencing is essential for the detection of targetable genomic alterations, immunohistochemistry (IHC) retains a practical role because it is widely available, rapid, and tissue-sparing. This narrative review summarizes the current and emerging role of predictive IHC in CCA, with emphasis on its clinical utility, interpretative pitfalls, and integration with molecular testing. HER2 and mismatch repair proteins currently represent the most relevant IHC-based markers in routine practice, albeit in selected subsets of patients. By contrast, PD-L1 has clear biological relevance but limited value as a stand-alone treatment selector in CCA, whereas Claudin 18.2 is promising but still investigational. Additional lines of research, including tumor microenvironment profiling, integrin-related pathways, and other theragnostic targets, may further refine patient selection, but these approaches are not yet standardized. Digital image analysis, radiomics, and machine learning are likely to improve quantification and may support future biomarker integration. A practical pathology-oriented approach should prioritize tissue stewardship, conservative interpretation of IHC results, and close coordination with molecular methods.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70230","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active Chlorine Antiseptics in Wound Care: Recent Advances and Perspectives for Military Medicine 活性氯消毒剂在伤口护理中的应用：军事医学的最新进展和展望。

IF 2.6 4区医学

Apmis Pub Date : 2026-06-02 DOI: 10.1111/apm.70228

Bohdan Murashevych, Dmytro Stepanskyi, William M. Wuest, Tim Holm Jakobsen

{"title":"Active Chlorine Antiseptics in Wound Care: Recent Advances and Perspectives for Military Medicine","authors":"Bohdan Murashevych, Dmytro Stepanskyi, William M. Wuest, Tim Holm Jakobsen","doi":"10.1111/apm.70228","DOIUrl":"10.1111/apm.70228","url":null,"abstract":"The spread of multidrug-resistant microorganisms remains a major global challenge that continues to worsen. Modern military conflicts intensify this problem by forcing healthcare systems to manage large numbers of patients with complex, heavily contaminated injuries in resource-limited settings. This can disrupt infection prevention and control and promote the emergence and spread of resistant pathogens. In this context, antiseptics are essential in combat wound management and should be used throughout patient care, from first aid to rehabilitation. Among them, active chlorine compounds deserve special attention because of their broad-spectrum, rapid microbicidal action, minimal risk of resistance development, good tolerability, and affordability. This review summarizes recent advances in their use for wounds of various origins. Active chlorine preparations are highly effective against microorganisms that commonly complicate acute and chronic wounds, including multidrug-resistant and biofilm-forming pathogens. In addition, their anti-inflammatory, reparative, and immunomodulatory properties may accelerate healing through multiple mechanisms while maintaining low toxicity. The broad range of formulations, from traditional hypochlorous acid solutions to polymers with N–Cl moieties and wearable electrochemical dressings, supports use in both field and hospital settings. Together, these features make active chlorine products promising non-antibiotic tools for modern wound infection control, particularly in military medicine.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70228","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148147915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review on the Logistics, Financial, Ethical, and Regulatory Frameworks of Artificial Intelligence in Digital Pathology 数字病理学中人工智能的物流、财务、伦理和监管框架综述

IF 2.6 4区医学

Apmis Pub Date : 2026-06-02 DOI: 10.1111/apm.70227

Yee Lin Tang, Daniel Dahlmeier

引用次数: 0

Histopathological Assessment of Precision-Cut Kidney Slices: A Live Human Tissue-Based Model of Renal Fibrosis 精确切肾片的组织病理学评估：活体肾纤维化模型。

IF 2.6 4区医学

Apmis Pub Date : 2026-05-29 DOI: 10.1111/apm.70226

Andreas Riishede, Camilla Merrild, Seulgi Hyun, Mia G. Madsen, Anna K. Keller, Henricus A. M. Mutsaers, Rikke Nørregaard, Lene N. Nejsum

{"title":"Histopathological Assessment of Precision-Cut Kidney Slices: A Live Human Tissue-Based Model of Renal Fibrosis","authors":"Andreas Riishede, Camilla Merrild, Seulgi Hyun, Mia G. Madsen, Anna K. Keller, Henricus A. M. Mutsaers, Rikke Nørregaard, Lene N. Nejsum","doi":"10.1111/apm.70226","DOIUrl":"10.1111/apm.70226","url":null,"abstract":"Chronic kidney disease (CKD) is characterized by a progressive decline of renal function. A major challenge in CKD drug development is the low success rate of drug candidates, with fewer than 10% of drugs entering Phase I clinical trials demonstrating clinical benefit. Traditional in vitro and in vivo models, while valuable, do not fully recapitulate human kidney complexity. To address this, we utilized human precision-cut kidney slices (PCKS), an ex vivo model that preserves human cellular diversity and organ architecture. Although this model lacks factors such as blood flow and osmotic gradients, it enables controlled induction of fibrosis through TGF-β treatment. To assess potential therapeutic interventions, we adapted a histopathological scoring system that was originally developed for human kidney biopsies. Our findings demonstrate that PCKS exhibit progressive fibrosis and tubular atrophy during culture up to 48 h, with TGF-β treatment further exacerbating these effects at the 48 h time-point. In contrast to PCKS from healthy patients, 48 h incubation of PCKS from CKD patients did not induce additional fibrosis or tubular atrophy. Thus, our scoring system provides a robust method for assessing fibrosis and tubular atrophy in human PCKS and may serve as a promising tool in preclinical drug screening.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70226","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UE, CT, and ACR TI-RADS Grading in the Combined Diagnosis of Benign and Malignant Thyroid Nodules UE、CT和ACR TI-RADS分级在良恶性甲状腺结节联合诊断中的应用。

IF 2.6 4区医学

Apmis Pub Date : 2026-05-29 DOI: 10.1111/apm.70221

Haijun Li, Yanan Xie, Shujun Wang, Daiwei Wang

{"title":"UE, CT, and ACR TI-RADS Grading in the Combined Diagnosis of Benign and Malignant Thyroid Nodules","authors":"Haijun Li, Yanan Xie, Shujun Wang, Daiwei Wang","doi":"10.1111/apm.70221","DOIUrl":"10.1111/apm.70221","url":null,"abstract":"<div>\u0000 \u0000 Accurate differentiation of benign and malignant thyroid nodules is crucial for clinical decisions, and multimodal imaging may outperform single modalities. We evaluated the clinical value of combining ultrasound elastography (UE), computed tomography (CT), and the American College of Radiology Thyroid Imaging Reporting and Data System (ACR TI-RADS) for distinguishing benign from malignant thyroid nodules. A single-center retrospective analysis of 80 consecutive patients with 100 thyroid nodules was conducted. All patients received ACR TI-RADS, UE, and CT, with surgical pathology as the gold standard. Sensitivity, specificity, accuracy, and pathological agreement were compared among individual tests and their combination. Pathology confirmed 31 malignant and 69 benign nodules. TI-RADS, UE, and CT showed moderate agreement with pathology (Kappa = 0.505, 0.530, 0.572), while the combined approach showed improved agreement (Kappa = 0.838) with fewer misdiagnoses and missed diagnoses. Cochran's Q test showed no significant difference in diagnostic accuracy among TI-RADS grading, UE, CT, and their combined use for benign and malignant thyroid nodules (p > 0.001). Combined use of UE, CT, and TI-RADS grading for diagnosing benign and malignant thyroid nodules improves consistency with pathological results, reduces misdiagnosis and missed diagnosis rates, and offers higher diagnostic value than single examinations.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.6 4区医学

Apmis Pub Date : 2026-05-21

引用次数: 0

IF 2.6 4区医学

Apmis Pub Date : 2026-05-21

引用次数: 0

IF 2.6 4区医学

Apmis Pub Date : 2026-05-21

引用次数: 0

IF 2.6 4区医学

Apmis Pub Date : 2026-05-21