IF 2.2 4区医学

Apmis Pub Date : 2025-01-16 DOI: 10.1111/apm.13509

Kasper Ingerslev, Tim Svenstrup Poulsen, Mikael Lenz Strube, Wojciech Skovrider-Ruminski, Doris Schledermann, Tine Henrichsen Schnack, Claus Høgdall, Jan Blaakær, Estrid Høgdall

{"title":"No Bacterial Biomass Detected in Tissue From Patients With Ovarian Cancer and Serous Tubal Intraepithelial Carcinomas Using 16S rDNA Sequencing","authors":"Kasper Ingerslev, Tim Svenstrup Poulsen, Mikael Lenz Strube, Wojciech Skovrider-Ruminski, Doris Schledermann, Tine Henrichsen Schnack, Claus Høgdall, Jan Blaakær, Estrid Høgdall","doi":"10.1111/apm.13509","DOIUrl":"10.1111/apm.13509","url":null,"abstract":"The ovarian oncobiome is subject to increasing scientific focus, but a potential link between bacterial dysbiosis and ovarian carcinogenesis remains controversial. Our primary aim was to characterize the bacterial microbiota in epithelial ovarian cancer samples. Secondarily, we aimed to compare results from the bacterial microbiota in formalin-fixed, paraffin-embedded ovarian tissue samples from 194 patients with epithelial ovarian cancer, fallopian tube tissue samples from 16 patients with serous tubal intraepithelial carcinomas and in benign fallopian tube tissue samples from 25 patients. Bacterial abundance was investigated by means of 16S rDNA Next Generation Sequencing. The 16S rDNA sequencing run produced a very low number of bacterial reads. Only seven samples displayed bacterial reads above 5000. Validation of detected bacterial reads by qPCR was negative and indicative of results being background amplification. Our results indicate no amount of bacterial biomass in the ovarian cancer, benign fallopian tube and in the samples with serous tubal intraepithelial carcinomas. The findings underline the importance of validating results from bacterial microbiota studies with additional technical assays before any conclusion may be drawn.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigen Specificity: A Fluctuating Aspect in the Development of Clinical Antibodies? 抗原特异性：临床抗体发展的波动方面？

IF 2.2 4区医学

Apmis Pub Date : 2025-01-14 DOI: 10.1111/apm.13515

Sandeep, Suraj H. Shinde, Abhay H. Pande

{"title":"Antigen Specificity: A Fluctuating Aspect in the Development of Clinical Antibodies?","authors":"Sandeep, Suraj H. Shinde, Abhay H. Pande","doi":"10.1111/apm.13515","DOIUrl":"10.1111/apm.13515","url":null,"abstract":"<div>\u0000 \u0000 Development of antibodies for clinical use is a complex process involving numerous aspects, with antigen specificity being the most important. Initially, polyclonal antibodies, that can recognize multiple specific and nonspecific antigens (polyreactive), were developed and were very effective in the treatments. Later on, the polyspecificity/polyreactivity of these polyclonal antibodies (binding to multiple antigens) raised concerns about therapeutic efficacy because of their nonspecific interactions and challenges, such as development of immune complexes, batch-to-batch variability. This highlighted the need for more targeted approaches. It was resolved by the marked invention of hybridoma technology in 1975 which resulted in the revolution in the antibody development field by offering monoclonal monospecific antibodies (bind single antigen). However, their limited application in complex pathologies sparked a paradigm shift, leading to the resurgence of polyspecific antibodies in the form of monoclonal polyspecific antibodies (Polybodies), which bind multiple antigens, but specifically. Till today, 14 Polybodies are approved for clinical use. This fluctuation in antigen specificity is directing the evolution of engineered antibodies that are going to drive the biopharmaceutical sector in the coming years. Through this write-up, we assert the fluctuating nature of antigen specificity during the antibody development and how it will be crucial for advancing biologics.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical Steps in Shotgun Metagenomics-Based Diagnosis of Bloodstream Infections Using Nanopore Sequencing 使用纳米孔测序的基于散弹枪宏基因组学的血流感染诊断的关键步骤。

IF 2.2 4区医学

Apmis Pub Date : 2025-01-14 DOI: 10.1111/apm.13511

Amelia Björnberg, David Nestor, Nilay Peker, Bhanu Sinha, Natacha Couto, John Rossen, Martin Sundqvist, Paula Mölling

{"title":"Critical Steps in Shotgun Metagenomics-Based Diagnosis of Bloodstream Infections Using Nanopore Sequencing","authors":"Amelia Björnberg, David Nestor, Nilay Peker, Bhanu Sinha, Natacha Couto, John Rossen, Martin Sundqvist, Paula Mölling","doi":"10.1111/apm.13511","DOIUrl":"10.1111/apm.13511","url":null,"abstract":"Shotgun metagenomics offers a broad detection of pathogens for rapid blood stream infection of pathogens but struggles with often low numbers of pathogens combined with high levels of human background DNA in clinical samples. This study aimed to develop a shotgun metagenomics protocol using blood spiked with various bacteria and to assess bacterial DNA extraction efficiency with human DNA depletion. The Blood Pathogen Kit (Molzym) was used to extract DNA from EDTA-whole blood (WB) and plasma samples, using contrived blood specimens spiked with bacteria for shotgun metagenomics diagnostics via Oxford Nanopore sequencing and PCR-based library preparation. Results showed that bacterial reads were higher in WB than plasma. Differences for Staphylococcus aureus and Streptococcus pneumoniae were more pronounced compared to Escherichia coli. Plasma samples exhibited better method reproducibility, with more consistent droplet digital PCR results for human DNA. The study found that extraction was more efficient for Gram-positive bacteria than Gram-negative, suggesting that the human DNA depletion exerts a negative effect on Gram-negative bacteria. Overall, shotgun metagenomics needs further optimisation to improve bacterial DNA recovery and enhance pathogen detection sensitivity. This study highlights some critical steps in the methodology of shotgun metagenomic-based diagnosis of blood stream infections using Nanopore sequencing.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Characteristics and Laboratory Diagnoses of Pediatric Patients Hospitalized During an Outbreak of Mycoplasma pneumoniae 肺炎支原体暴发期间儿科住院患者的临床特点和实验室诊断

IF 2.2 4区医学

Apmis Pub Date : 2025-01-10 DOI: 10.1111/apm.13514

Tao Meng, Yuanhong Xu

{"title":"Clinical Characteristics and Laboratory Diagnoses of Pediatric Patients Hospitalized During an Outbreak of Mycoplasma pneumoniae","authors":"Tao Meng, Yuanhong Xu","doi":"10.1111/apm.13514","DOIUrl":"10.1111/apm.13514","url":null,"abstract":"<div>\u0000 \u0000 This study prospectively collected the clinical data, information on respiratory pathogens, and laboratory findings of children with Mycoplasma pneumoniae (M. pneumonia) infection who were hospitalized at the First Affiliated Hospital of Anhui Medical University during the M. pneumoniae outbreak in Hefei City, Anhui Province, China, between October 2023 and December 2023. We analyzed the prevalence of M. pneumoniae infection in hospitalized children during the M. pneumoniae outbreak, discrepancies in the detection of M. pneumoniae by multiplex polymerase chain reaction (PCR) and serological methods, and the differences in clinical characteristics and laboratory results of patients with co-infections of M. pneumoniae and other pathogens with the aim of providing a reference for disease assessment and clinical treatment of M. pneumoniae. A total of 270 children, 146 males and 124 females, were enrolled in the study after screening with the inclusion criteria. The most common co-infections were adenovirus (ADV) (48 cases) and influenza A virus (FLU A) (30 cases). The prevalence of infections was higher in children under the age of 7 years (54.1%). In addition, 167 of the PCR-positive patients were infected with macrolide-resistant Mycoplasma pneumoniae (MRMP), suggesting that MRMP infections are common in hospitalized children. Levels of procalcitonin (PCT) (OR 15.765 [95% CI, 1.651–150.533], p < 0.017) and lactate dehydrogenase (LDH) (OR 1.006 [95% CI, 1.003–1.009], p < 0.001) were independently predictive of co-infections in patients infected with M. pneumoniae.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactams Exhibit Potent Antifungal Activity Against Monospecies and Multispecies Interkingdom Biofilms on a Novel Hydrogel Skin Model 在一种新型水凝胶皮肤模型上，内酰胺类对单物种和多物种间生物膜显示出有效的抗真菌活性。

IF 2.2 4区医学

Apmis Pub Date : 2025-01-10 DOI: 10.1111/apm.13510

Hafsa Abduljalil, Om Alkhir Alshanta, Safa Chougule, Mark Butcher, Bryn Short, William McLean, Neil Parry, Joanne O'Keeffe, Gordon Ramage

{"title":"Lactams Exhibit Potent Antifungal Activity Against Monospecies and Multispecies Interkingdom Biofilms on a Novel Hydrogel Skin Model","authors":"Hafsa Abduljalil, Om Alkhir Alshanta, Safa Chougule, Mark Butcher, Bryn Short, William McLean, Neil Parry, Joanne O'Keeffe, Gordon Ramage","doi":"10.1111/apm.13510","DOIUrl":"10.1111/apm.13510","url":null,"abstract":"Infections of intact and damaged skin barriers and keratin are frequently associated with complex biofilm communities containing bacteria and fungi, yet there are limited options for successful management. This study intended to focus on the utility of some novel proprietary lactam molecules, quorum sensing (QS)–derived halogenated furanones, which act to block the QS pathway, against key fungal pathogens of the skin (Candida albicans, Malassezia furfur and Microsporum gypseum). Moreover, we aimed to assess how these actives performed against complex interkingdom biofilms in a clinically relevant model. Two lactam derivatives were tested against a panel of important fungal pathogens and then quantitatively assessed against simple and increasingly complex interkingdom biofilm models on polystyrene coverslips and a novel keratin hydrogel system. The lactams were shown to be effective against a wide range of fungal species in the planktonic and biofilm forms, with no ability to regrow. The fungal component of the multispecies biofilm models was significantly reduced with lactam treatment. Lactam treatment was also comparably effective compared to the non-prescription topical antifungal ‘Lamisil’ against C. albicans early and late biofilms. This study highlights the effectiveness of lactams as a novel antimicrobial for the management of the polymicrobial and interkingdom multispecies biofilms.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11718591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Metabolic Drives Affecting Th17/Treg Gene Expression Changes and Differentiation: Impact on Immune-Microenvironment Regulation” 对“影响Th17/Treg基因表达变化和分化的代谢驱动：对免疫微环境调节的影响”的更正。

IF 2.2 4区医学

Apmis Pub Date : 2025-01-10 DOI: 10.1111/apm.13513

引用次数: 0

Echinacoside attenuates Klebsiella pneumoniae-induced pneumonia via inhibition of the TLR4/NF-κB signaling 紫锥菊苷通过抑制TLR4/NF-κB信号通路减轻肺炎克雷伯菌诱导的肺炎。

IF 2.2 4区医学

Apmis Pub Date : 2025-01-05 DOI: 10.1111/apm.13507

Mi Zhang, Ming Zhan, Xinyu Song

{"title":"Echinacoside attenuates Klebsiella pneumoniae-induced pneumonia via inhibition of the TLR4/NF-κB signaling","authors":"Mi Zhang, Ming Zhan, Xinyu Song","doi":"10.1111/apm.13507","DOIUrl":"10.1111/apm.13507","url":null,"abstract":"The Gram-negative bacterium Klebsiella pneumoniae (K. pneumoniae) is one major causative agent of community- and hospital-acquired pneumonia. Echinacoside (ECH) is a phenylethanoid glycoside isolated from Cistanche deserticola that possesses anti-inflammatory activity. Our research aimed to confirm whether ECH alleviates K. pneumoniae-induced pneumonia and explore the underlying regulatory mechanisms. BEAS-2B cells and BALB/c mice were infected by K. pneumoniae to establish the cellular and animal models, respectively, followed by ECH treatment. Inflammatory cytokine levels were detected by RT-qPCR and ELISA. The lung wet/dry (W/D) weight ratio and the myeloperoxidase (MPO) activity in lung tissues were examined. The pulmonary histopathologic changes were observed through hematoxylin and eosin (H&E) staining. The levels of TLR4/NF-κB pathway-associated molecules were estimated through western blotting, immunohistochemical, and immunohistochemical staining. K. pneumoniae infection caused lung histopathologic damage, enhanced MPO activity, elevated lung W/D weight ratio, and upregulated inflammatory cytokine levels in mice and promoted inflammatory cytokine expression in BEAS-2B cells, which were reversed by ECH treatment. K. pneumoniae infection-induced upregulation in TLR4, phosphorylated (p)-p65, and p-IκBα levels, and downregulation in IκBα levels in BEAS-2B cells and pneumonia mice were overturned by ECH treatment. ECH ameliorates K. pneumoniae-induced pneumonia through suppressing the TLR4/NF-κB pathway.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monkeypox virus infection in pregnancy: description of two cases reported to the Colombian National Institute of Health 妊娠期猴痘病毒感染：对向哥伦比亚国家卫生研究所报告的两例病例的描述

IF 2.2 4区医学

Apmis Pub Date : 2024-12-26 DOI: 10.1111/apm.13488

Mercado-Reyes Marcela, Daza Marcela, Rodríguez Peña Yazmín, Araque Muñoz Paula, Franco-Muñoz Carlos, Ruíz-Moreno Hector, Corchuelo Chavarro Sheryll, Portilla Velandia Claudia, Osorio Usaquén Johana, Ávila Mellizo Greace, Narvaez Díaz Nubia, Walteros Diana, Cortes Palacio Katherinne, Piñeros Daza José Ángel

引用次数: 0

Efficacy of two novel antifungal lipopeptides, AF4 and AF5 of bacillomycin D family in murine models of invasive candidiasis, cryptococcosis, and aspergillosis 芽孢杆菌素D家族两种新型抗真菌脂肽AF4和AF5对侵袭性念珠菌病、隐球菌病和曲霉病小鼠模型的疗效

IF 2.2 4区医学

Apmis Pub Date : 2024-12-25 DOI: 10.1111/apm.13506

Raees A. Paul, Madhuri Madduri, Nisha Kashyap, Swetha Ramesh, Shivaprakash M. Rudramurthy, Utpal Roy

{"title":"Efficacy of two novel antifungal lipopeptides, AF4 and AF5 of bacillomycin D family in murine models of invasive candidiasis, cryptococcosis, and aspergillosis","authors":"Raees A. Paul, Madhuri Madduri, Nisha Kashyap, Swetha Ramesh, Shivaprakash M. Rudramurthy, Utpal Roy","doi":"10.1111/apm.13506","DOIUrl":"10.1111/apm.13506","url":null,"abstract":"Invasive fungal diseases are an important public health concern due to an increase in the at-risk population and high mortality associated with these infections. Managing invasive fungal infections poses a significant challenge given the limited antifungal options and the emergence of resistance in key fungal pathogens. Through a comprehensive approach, we evaluated the in vitro antifungal activity and the in vivo efficacy of two novel lipopeptides, AF4 and AF5 in murine models of disseminated candidiasis, cryptococcosis, and aspergillosis. Flow cytometry analysis with propidium iodide showed a dose-dependent increase in the permeability and disruption of fungal cell membranes, underscoring the membrane perturbing effects of AF4 and AF5. These observations were further substantiated by SEM analyses that showed yeast cell and hyphal deformation and leakage of cellular contents. Our in vivo investigations utilizing two doses (5 and 10 mg/kg bodyweight) of each lipopeptide and its comparison with standard antifungal therapies showed lipopeptides significantly improved the odds of mice survival in invasive candidiasis and cryptococcosis models, with a reduction in organ fungal burden by 2 to 3-log10 order. Additionally, in the disseminated aspergillosis model, treatment with 10 mg/kg of AF4 significantly improved median survival from 4 to 10 days while achieving a notable 1-log10 order reduction in organ fungal burden. Overall, our study underscores the potent and broad-spectrum antifungal activity of lipopeptides in mouse infection models, hinting at their promising therapeutic potential in invasive fungal disease.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review and meta-analysis: rising prevalence of colistin resistance in ICU-acquired Gram-negative bacteria 一项系统综述和荟萃分析：icu获得性革兰氏阴性菌中粘菌素耐药性的患病率上升。

IF 2.2 4区医学

Apmis Pub Date : 2024-12-22 DOI: 10.1111/apm.13508

Na Li, Elaheh Ebrahimi, Mohammad Sholeh, Reyhane Dousti, Ebrahim Kouhsari

{"title":"A systematic review and meta-analysis: rising prevalence of colistin resistance in ICU-acquired Gram-negative bacteria","authors":"Na Li, Elaheh Ebrahimi, Mohammad Sholeh, Reyhane Dousti, Ebrahim Kouhsari","doi":"10.1111/apm.13508","DOIUrl":"10.1111/apm.13508","url":null,"abstract":"Colistin is a last-resort treatment for multidrug-resistant Gram-negative bacterial infections, particularly in critically ill patients. Nevertheless, it remains a major threat to public health. We assessed the proportion of colistin-resistant Gram-negative isolates from intensive care unit (ICU) infections in different years, areas, pathogens, and antimicrobial susceptibility tests (AST). We searched the studies in PubMed, Scopus, Embase, and Web of Science (until November 2021). Statistical analyses were conducted using STATA software (ver. 14.0). The overall rate of colistin resistance was 5.18% (95% CI 2.70%–8.22%). The proportion of colistin resistance was 4% (95% CI 2%–7%) before 2015 and 6% (95% CI 4%–9%) in 2015–2019. The rates of colistin resistance in Europe, America, Asia, and Africa were 8.24%, 3.78%, 3.60%, and 0%, respectively. The proportion of colistin-resistant non-fermenting Gram-negative bacilli isolated from the ICU was 2.25% (Acinetobacter baumannii [1.68%] and Pseudomonas aeruginosa [3.30%]). A 4-fold increase in colistin resistance was observed when comparing EUCAST and CLSI. We described the global epidemiology of colistin resistance over time and shown the distribution of colistin-resistant strains in different countries. Robust antimicrobial stewardship programs can increase the success of ICU physicians in improving patient outcomes.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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