IF 2.2 4区医学

Apmis Pub Date : 2025-01-10 DOI: 10.1111/apm.13513

引用次数: 0

Echinacoside attenuates Klebsiella pneumoniae-induced pneumonia via inhibition of the TLR4/NF-κB signaling 紫锥菊苷通过抑制TLR4/NF-κB信号通路减轻肺炎克雷伯菌诱导的肺炎。

IF 2.2 4区医学

Apmis Pub Date : 2025-01-05 DOI: 10.1111/apm.13507

Mi Zhang, Ming Zhan, Xinyu Song

{"title":"Echinacoside attenuates Klebsiella pneumoniae-induced pneumonia via inhibition of the TLR4/NF-κB signaling","authors":"Mi Zhang, Ming Zhan, Xinyu Song","doi":"10.1111/apm.13507","DOIUrl":"10.1111/apm.13507","url":null,"abstract":"The Gram-negative bacterium Klebsiella pneumoniae (K. pneumoniae) is one major causative agent of community- and hospital-acquired pneumonia. Echinacoside (ECH) is a phenylethanoid glycoside isolated from Cistanche deserticola that possesses anti-inflammatory activity. Our research aimed to confirm whether ECH alleviates K. pneumoniae-induced pneumonia and explore the underlying regulatory mechanisms. BEAS-2B cells and BALB/c mice were infected by K. pneumoniae to establish the cellular and animal models, respectively, followed by ECH treatment. Inflammatory cytokine levels were detected by RT-qPCR and ELISA. The lung wet/dry (W/D) weight ratio and the myeloperoxidase (MPO) activity in lung tissues were examined. The pulmonary histopathologic changes were observed through hematoxylin and eosin (H&E) staining. The levels of TLR4/NF-κB pathway-associated molecules were estimated through western blotting, immunohistochemical, and immunohistochemical staining. K. pneumoniae infection caused lung histopathologic damage, enhanced MPO activity, elevated lung W/D weight ratio, and upregulated inflammatory cytokine levels in mice and promoted inflammatory cytokine expression in BEAS-2B cells, which were reversed by ECH treatment. K. pneumoniae infection-induced upregulation in TLR4, phosphorylated (p)-p65, and p-IκBα levels, and downregulation in IκBα levels in BEAS-2B cells and pneumonia mice were overturned by ECH treatment. ECH ameliorates K. pneumoniae-induced pneumonia through suppressing the TLR4/NF-κB pathway.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monkeypox virus infection in pregnancy: description of two cases reported to the Colombian National Institute of Health 妊娠期猴痘病毒感染：对向哥伦比亚国家卫生研究所报告的两例病例的描述

IF 2.2 4区医学

Apmis Pub Date : 2024-12-26 DOI: 10.1111/apm.13488

Mercado-Reyes Marcela, Daza Marcela, Rodríguez Peña Yazmín, Araque Muñoz Paula, Franco-Muñoz Carlos, Ruíz-Moreno Hector, Corchuelo Chavarro Sheryll, Portilla Velandia Claudia, Osorio Usaquén Johana, Ávila Mellizo Greace, Narvaez Díaz Nubia, Walteros Diana, Cortes Palacio Katherinne, Piñeros Daza José Ángel

引用次数: 0

Efficacy of two novel antifungal lipopeptides, AF4 and AF5 of bacillomycin D family in murine models of invasive candidiasis, cryptococcosis, and aspergillosis 芽孢杆菌素D家族两种新型抗真菌脂肽AF4和AF5对侵袭性念珠菌病、隐球菌病和曲霉病小鼠模型的疗效

IF 2.2 4区医学

Apmis Pub Date : 2024-12-25 DOI: 10.1111/apm.13506

Raees A. Paul, Madhuri Madduri, Nisha Kashyap, Swetha Ramesh, Shivaprakash M. Rudramurthy, Utpal Roy

{"title":"Efficacy of two novel antifungal lipopeptides, AF4 and AF5 of bacillomycin D family in murine models of invasive candidiasis, cryptococcosis, and aspergillosis","authors":"Raees A. Paul, Madhuri Madduri, Nisha Kashyap, Swetha Ramesh, Shivaprakash M. Rudramurthy, Utpal Roy","doi":"10.1111/apm.13506","DOIUrl":"10.1111/apm.13506","url":null,"abstract":"Invasive fungal diseases are an important public health concern due to an increase in the at-risk population and high mortality associated with these infections. Managing invasive fungal infections poses a significant challenge given the limited antifungal options and the emergence of resistance in key fungal pathogens. Through a comprehensive approach, we evaluated the in vitro antifungal activity and the in vivo efficacy of two novel lipopeptides, AF4 and AF5 in murine models of disseminated candidiasis, cryptococcosis, and aspergillosis. Flow cytometry analysis with propidium iodide showed a dose-dependent increase in the permeability and disruption of fungal cell membranes, underscoring the membrane perturbing effects of AF4 and AF5. These observations were further substantiated by SEM analyses that showed yeast cell and hyphal deformation and leakage of cellular contents. Our in vivo investigations utilizing two doses (5 and 10 mg/kg bodyweight) of each lipopeptide and its comparison with standard antifungal therapies showed lipopeptides significantly improved the odds of mice survival in invasive candidiasis and cryptococcosis models, with a reduction in organ fungal burden by 2 to 3-log10 order. Additionally, in the disseminated aspergillosis model, treatment with 10 mg/kg of AF4 significantly improved median survival from 4 to 10 days while achieving a notable 1-log10 order reduction in organ fungal burden. Overall, our study underscores the potent and broad-spectrum antifungal activity of lipopeptides in mouse infection models, hinting at their promising therapeutic potential in invasive fungal disease.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review and meta-analysis: rising prevalence of colistin resistance in ICU-acquired Gram-negative bacteria 一项系统综述和荟萃分析：icu获得性革兰氏阴性菌中粘菌素耐药性的患病率上升。

IF 2.2 4区医学

Apmis Pub Date : 2024-12-22 DOI: 10.1111/apm.13508

Na Li, Elaheh Ebrahimi, Mohammad Sholeh, Reyhane Dousti, Ebrahim Kouhsari

{"title":"A systematic review and meta-analysis: rising prevalence of colistin resistance in ICU-acquired Gram-negative bacteria","authors":"Na Li, Elaheh Ebrahimi, Mohammad Sholeh, Reyhane Dousti, Ebrahim Kouhsari","doi":"10.1111/apm.13508","DOIUrl":"10.1111/apm.13508","url":null,"abstract":"Colistin is a last-resort treatment for multidrug-resistant Gram-negative bacterial infections, particularly in critically ill patients. Nevertheless, it remains a major threat to public health. We assessed the proportion of colistin-resistant Gram-negative isolates from intensive care unit (ICU) infections in different years, areas, pathogens, and antimicrobial susceptibility tests (AST). We searched the studies in PubMed, Scopus, Embase, and Web of Science (until November 2021). Statistical analyses were conducted using STATA software (ver. 14.0). The overall rate of colistin resistance was 5.18% (95% CI 2.70%–8.22%). The proportion of colistin resistance was 4% (95% CI 2%–7%) before 2015 and 6% (95% CI 4%–9%) in 2015–2019. The rates of colistin resistance in Europe, America, Asia, and Africa were 8.24%, 3.78%, 3.60%, and 0%, respectively. The proportion of colistin-resistant non-fermenting Gram-negative bacilli isolated from the ICU was 2.25% (Acinetobacter baumannii [1.68%] and Pseudomonas aeruginosa [3.30%]). A 4-fold increase in colistin resistance was observed when comparing EUCAST and CLSI. We described the global epidemiology of colistin resistance over time and shown the distribution of colistin-resistant strains in different countries. Robust antimicrobial stewardship programs can increase the success of ICU physicians in improving patient outcomes.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fritz Kauffmann: innovator in microbial classification 弗里茨·考夫曼：微生物分类的创新者。

IF 2.2 4区医学

Apmis Pub Date : 2024-12-22 DOI: 10.1111/apm.13504

Rie Jønsson, Mathias Mølbak Ingholt, Karen Angeliki Krogfelt

引用次数: 0

Fungal positivity and other laboratory parameters in HIV- and non-HIV-related cryptococcal meningitis patients upon antifungal treatment 抗真菌治疗后HIV和非HIV相关隐球菌性脑膜炎患者的真菌阳性和其他实验室参数

IF 2.2 4区医学

Apmis Pub Date : 2024-12-16 DOI: 10.1111/apm.13476

Chaojuan Liang, Shaoyong Xi, Hanzhen Su, Lida Mo, Liejun Jiang, Huan Lu, Huayi Huang

{"title":"Fungal positivity and other laboratory parameters in HIV- and non-HIV-related cryptococcal meningitis patients upon antifungal treatment","authors":"Chaojuan Liang, Shaoyong Xi, Hanzhen Su, Lida Mo, Liejun Jiang, Huan Lu, Huayi Huang","doi":"10.1111/apm.13476","DOIUrl":"10.1111/apm.13476","url":null,"abstract":"The association between fungal positivity in cerebral spinal fluid (CSF) and other laboratory parameters in cryptococcal meningitis (CM) with or without HIV infection is unclear. India ink staining and culture were used to detect the Cryptococcus in the CSF during the treatment course. Hematology analysis and chemistry analysis of CSF were also performed. Flow cytometry was used to analyze the T lymphocyte subsets in the blood. The positivity of the culture reduced significantly faster than that of the ink staining in both HIV and non-HIV patients between treatment time points. The total protein in the CSF of the HIV-related patients was significantly lower than in the non-HIV-related patients at all time points of treatment (p = 0.009, 0.012, 0.001, and 0.037, respectively). The lactate dehydrogenase (LDH) in the CSF of the HIV-related patients at admission was significantly lower than in the non-HIV-related patients (p = 0.017). There were significant differences in glucose and LDH levels between different time points of treatment (p = 0.000 and 0.016, respectively) in the non-HIV-related patients. For Cryptococcus detection in CSF, the culture method appeared to be more sensitive and reliable than the ink staining method. HIV-related CM patients showed certain hematologic and CSF chemistry features which may help guide the management of patients.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of toll-like receptors in recurrent pleomorphic adenoma and carcinoma ex pleomorphic adenoma 复发性多形性腺瘤和多形性腺瘤癌的收费样受体表达。

IF 2.2 4区医学

Apmis Pub Date : 2024-12-16 DOI: 10.1111/apm.13505

Maria K. Jauhiainen, Anttoni Markkanen, Timo Atula, Katri Aro, Caj Haglund, Maria Söderlund-Venermo, Antti A. Mäkitie, Saku T. Sinkkonen, Jaana Hagström

{"title":"Expression of toll-like receptors in recurrent pleomorphic adenoma and carcinoma ex pleomorphic adenoma","authors":"Maria K. Jauhiainen, Anttoni Markkanen, Timo Atula, Katri Aro, Caj Haglund, Maria Söderlund-Venermo, Antti A. Mäkitie, Saku T. Sinkkonen, Jaana Hagström","doi":"10.1111/apm.13505","DOIUrl":"10.1111/apm.13505","url":null,"abstract":"Pleomorphic adenoma (PA) is a benign salivary gland tumour that may recur or undergo malignant transformation (CXPA). Toll-like receptors (TLR) mediate immune responses triggered by various agents such as viruses and are related to tumour formation either by stimulating or suppressing their growth, with variation across different tumour entities. We compared TLR immunohistochemical expression in PA, its recurrent counterparts and CXPA and evaluated the effect of virus presence in these tumours. We studied the expression of TLR-2, -3, -5, -7 and -9 in 25 PA, 34 recurrent PA and 15 CXPA tumour samples. In addition, we examined the TLR expression levels in the presence and absence of herpes-, polyoma- and parvovirus DNA in a subset of tumours (n = 20). CXPA expressed significantly more TLR-5 and TLR-9 in the nucleus, cytoplasm and cell membrane compared with benign PA. The presence of virus DNA did not notably affect the TLR expression. TLR expression patterns seem to reflect tumour behaviour but are independent of the presence of viruses tested in this study.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.13505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic impact of subepithelial Helicobacter pylori infection on clinical outcomes in patients with dyspepsia 上皮下幽门螺杆菌感染对消化不良患者临床预后的影响。

IF 2.2 4区医学

Apmis Pub Date : 2024-11-27 DOI: 10.1111/apm.13503

Chalermpak Supakatitham, Kongsak Loharamtaweethong

{"title":"Prognostic impact of subepithelial Helicobacter pylori infection on clinical outcomes in patients with dyspepsia","authors":"Chalermpak Supakatitham, Kongsak Loharamtaweethong","doi":"10.1111/apm.13503","DOIUrl":"10.1111/apm.13503","url":null,"abstract":"Several in vivo and in vitro studies have shown that Helicobacter pylori can invade epithelial cells and the lamina propria, potentially leading to underdiagnosis due to its subepithelial location. This retrospective study investigated H. pylori infection patterns and their impact on clinical improvement. Gastric tissue biopsies from 346 patients (August to December 2021) were studied using four commercially available immunohistochemical antibodies (TMDU, BioGenex, Cell Marque, and DAKO). The bacteria were graded based on their surface epithelial and subepithelial locations and then combined to establish an overall pattern. BioGenex, the antibody with the highest diagnostic performance due to its superior detection of surface and subepithelial cases, was selected as the gold standard for determining study outcomes. The isolated subepithelial H. pylori pattern was found to be an independent unfavorable prognostic feature. Patients with this pattern had the worst clinical outcomes compared to groups with isolated surface epithelial or other mixed patterns, which did not significantly differ. Subepithelial H. pylori should be included in pathological reports alongside the updated Sydney System. Further research should explore whether its eradication could improve treatment outcomes.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of extracellular enzymes on Staphylococcus aureus host tissue adaptation and infection 细胞外酶对金黄色葡萄球菌宿主组织适应性和感染的影响。

IF 2.2 4区医学

Apmis Pub Date : 2024-11-27 DOI: 10.1111/apm.13502

Atlanta Borah, Anand Srivastava

引用次数: 0

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