IF 2.2 4区医学

Apmis Pub Date : 2026-07-20 DOI: 10.1111/apm.70237

Yuanyuan Zhang, Anni Xu, Huiling Qin, Dongcai Feng, Changyu Tian, Caiqin Lin

{"title":"miR-330-3p Inhibits the Development of Acute Myeloid Leukemia by Targeting SYTL4","authors":"Yuanyuan Zhang, Anni Xu, Huiling Qin, Dongcai Feng, Changyu Tian, Caiqin Lin","doi":"10.1111/apm.70237","DOIUrl":"https://doi.org/10.1111/apm.70237","url":null,"abstract":"<div>\u0000 \u0000 MicroRNAs (miRNAs) serve as crucial regulators in the development of various cancers. Studies have mentioned that miR-330-3p is reduced in Acute myeloid leukemia (AML). Our research objectives included determining the regulatory roles and underlying mechanisms of miR-330-3p in AML progression. miR-330-3p expression in AML was quantified through qRT-PCR. The clinical relevance of miR-330-3p in AML progression, diagnosis, and prognosis was assessed by chi-square, ROC, Cox, and Kaplan–Meier survival analysis. The impacts of miR-330-3p on AML cell function were examined by CCK8 and Transwell assay. The targeting relationship between miR-330-3p and SYTL4 was analyzed via database and luciferase assay. miR-330-3p was obviously reduced in AML. Low miR-330-3p expression was related to adverse clinical features of AML, suggesting AML progression. miR-330-3p performed well in differentiating AML patients from healthy individuals, and reduced miR-330-3p expression was significantly related to poorer survival outcomes in patients with AML. miR-330-3p upregulation potently inhibited AML cell proliferation, migration, and invasion. Furthermore, SYTL4 was a target gene of miR-330-3p. And rescue tests demonstrated that miR-330-3p and SYTL4 jointly participate in the development of AML. In conclusion, miR-330-3p inhibited the development of AML by targeting SYTL4; miR-330-3p may serve as a valuable prognosis and diagnostic biomarker for AML patients.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogenic Silver Nanoparticles Synthesised Using Staphylococcus saprophyticus Cell-Free Supernatant Exhibit Potent Antimicrobial Activities via Oxidative Stress 利用腐生葡萄球菌无细胞上清液合成的生物源银纳米颗粒通过氧化应激表现出强大的抗菌活性

IF 2.2 4区医学

Apmis Pub Date : 2026-07-20 DOI: 10.1111/apm.70239

Nidhi Chandrakar, Dugeshwar Karley, Sudhir K. Shukla

{"title":"Biogenic Silver Nanoparticles Synthesised Using Staphylococcus saprophyticus Cell-Free Supernatant Exhibit Potent Antimicrobial Activities via Oxidative Stress","authors":"Nidhi Chandrakar, Dugeshwar Karley, Sudhir K. Shukla","doi":"10.1111/apm.70239","DOIUrl":"https://doi.org/10.1111/apm.70239","url":null,"abstract":"In this study, we report the eco-friendly synthesis of silver nanoparticles (AgNPs) using cell-free supernatant of Staphylococcus saprophyticus as a biological reducing and stabilising agent. Nanoparticle formation was confirmed by visible colour change and UV–visible spectroscopy (λmax at 418 nm). Structural characterisation by SEM, XRD and FT-IR indicated predominantly spherical, crystalline, silver-containing nanoparticles associated with extracellular biomolecules that may contribute to stabilisation; XRD analysis indicated a surface phase dominated by silver halides (AgBr/AgCl) rather than confirming a purely metallic Ag0 lattice. The biosynthesised AgNPs exhibited strong antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa, with minimum inhibitory concentrations of 3 and 0.5 μg/mL, respectively. Significant antifungal activity was also observed against Candida albicans, with marked growth suppression at 100 μg/mL. The AgNPs effectively reduced biofilm formation, extracellular matrix production and virulence traits. DCFH-DA fluorescence and MTT assays showed increased reactive oxygen species generation and reduced cellular metabolic activity in treated microbes, suggesting oxidative stress-associated antimicrobial effects. Cytotoxicity testing indicated low toxicity towards HeLa cells at lower concentrations, with dose-dependent effects observed only at higher exposure levels. Overall, results demonstrate that biogenic AgNPs possess antibacterial, antifungal and antibiofilm properties, highlighting their potential as sustainable and eco-friendly antimicrobial candidates.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70239","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect and Clinical Characteristics of Human Cytomegalovirus Infection on Vascular Inflammation in Patients With Coronary Atherosclerotic Heart Disease 人巨细胞病毒感染对冠状动脉粥样硬化性心脏病患者血管炎症的影响及临床特点。

IF 2.2 4区医学

Apmis Pub Date : 2026-07-16 DOI: 10.1111/apm.70229

Hui Huang, Huan Liu, Ping Liu, Wangqiang Zhang

{"title":"Effect and Clinical Characteristics of Human Cytomegalovirus Infection on Vascular Inflammation in Patients With Coronary Atherosclerotic Heart Disease","authors":"Hui Huang, Huan Liu, Ping Liu, Wangqiang Zhang","doi":"10.1111/apm.70229","DOIUrl":"10.1111/apm.70229","url":null,"abstract":"<div>\u0000 \u0000 This study evaluated the impact of human cytomegalovirus (HCMV) infection on vascular inflammation and clinical characteristics in patients with coronary atherosclerotic heart disease (CHD). A total of 180 CHD patients with HCMV infection (research group) and 90 CHD patients without HCMV infection (control group) were enrolled. Serum levels of hs-CRP, TNF-α, IL-6, and Lp-PLA2 were measured, and clinical data were collected. The research group showed significantly higher inflammatory marker levels than controls (p < 0.05). Mean viral load was 4.58 ± 1.01 copies/mL, and high-load patients had higher marker levels than low-load patients (p < 0.05). Significant between-group differences were observed in CHD severity, number of diseased vessels, stenosis degree, cardiac function, angina grade, and carotid atherosclerosis (p < 0.05). HCMV viral load was positively correlated with all inflammatory markers and clinical severity indices (p < 0.05). These findings indicate that HCMV infection is associated with exacerbated vascular inflammation and worse CHD progression, suggesting the need for timely antiviral intervention.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can We Foresee Grade and Stage Progression in Mixed Low- and High-Grade Noninvasive Papillary Urothelial Carcinomas? 我们能否预测低级别和高级别非侵入性尿路上皮乳头状癌的分级和分期进展？

IF 2.2 4区医学

Apmis Pub Date : 2026-07-14 DOI: 10.1111/apm.70238

Berna Karabulut, Osman Dag, Kemal Kosemehmetoglu, Gunes Guner

{"title":"Can We Foresee Grade and Stage Progression in Mixed Low- and High-Grade Noninvasive Papillary Urothelial Carcinomas?","authors":"Berna Karabulut, Osman Dag, Kemal Kosemehmetoglu, Gunes Guner","doi":"10.1111/apm.70238","DOIUrl":"10.1111/apm.70238","url":null,"abstract":"<div>\u0000 \u0000 Noninvasive papillary urothelial carcinoma (NIPUC), currently classified in a two-tier system, frequently exhibits mixed low- and high-grade morphology. We aimed to determine the impact of histopathologic features on grade and stage progression in mixed NIPUCs. Primary and follow-up bladder specimens (n = 106) of 28 patients diagnosed with mixed NIPUC were retrieved. Pathological parameters included biopsy volume, percentages of 2-tier (low-high) and 4-tier (Grade 1–4) grades, discohesion, and whirling. Outcome measures were grade trend (decline or incline) and stage progression. The mean age was 65 (range, 35–81); all but two were male. Ten patients had a previous biopsy showing pure low-grade NIPUC. Patients with > 5% high-grade areas (n = 14) were classified as high-grade. The mean distributions of G1-G4 in mixed NIPUCs at zero-point were 42%, 42%, 15%, and 1%, respectively. Tumors with > 85% Grade 1 histology didn't exhibit an upward grade trend. Stage progression was significantly associated with tumors containing ≥ 5% Grades 3–4 (high-grade) areas (p = 0.031). Grade trend was directly correlated with stage progression (p = 0.012). Mixed NIPUC has heterogeneous clinical outcomes: a high proportion of Grade 1 areas (> 85%) predicts a non-inclining grade trend, whereas the presence of ≥ 5% high-grade (Grades 3 and 4) morphology suggests future stage progression.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Hormonal Contraceptives on Circulating Biomarkers of Inflammation, Chemotaxis, Angiogenesis, and Vascular Stress 激素避孕药对炎症、趋化性、血管生成和血管应激循环生物标志物的影响。

IF 2.2 4区医学

Apmis Pub Date : 2026-07-14 DOI: 10.1111/apm.70235

Bertram Kjerulff, Khoa Manh Dinh, Jens Kjærgaard Boldsen, Ulrik Schiøler Kesmodel, Joseph Dowsett, Susan Mikkelsen, Lisette J. A. Kogelman, Maria Didriksen, Christina Mikkelsen, Jakob Bay, Mie Topholm Bruun, Bitten Aagaard, Mette Nyegaard, Klaus Rostgaard, Erik Sørensen, Ole Birger Pedersen, Sisse Rye Ostrowski, Christian Erikstrup

{"title":"Effect of Hormonal Contraceptives on Circulating Biomarkers of Inflammation, Chemotaxis, Angiogenesis, and Vascular Stress","authors":"Bertram Kjerulff, Khoa Manh Dinh, Jens Kjærgaard Boldsen, Ulrik Schiøler Kesmodel, Joseph Dowsett, Susan Mikkelsen, Lisette J. A. Kogelman, Maria Didriksen, Christina Mikkelsen, Jakob Bay, Mie Topholm Bruun, Bitten Aagaard, Mette Nyegaard, Klaus Rostgaard, Erik Sørensen, Ole Birger Pedersen, Sisse Rye Ostrowski, Christian Erikstrup","doi":"10.1111/apm.70235","DOIUrl":"10.1111/apm.70235","url":null,"abstract":"Hormonal contraceptive (HC) use is known to be associated with elevated CRP levels. However, evidence on the effect of HC on several circulating inflammatory biomarkers in healthy humans is limited. The aim of this study was to examine the association between hormonal contraceptives and circulating biomarker levels and differential cell counts. A cross-sectional observational study design with biomarker measurements from 4,160 healthy females was used, coupled with registered HC prescriptions. We compared 752 high and low dose combined oral contraceptive (COC) users, 77 progestin-only pill users, 417 levonorgestrel intrauterine device users, 65 hormonal therapy users, and 1,523 post-menopausal women with a control group of 1,326 women below 50 years. Repeated differential cell counts from a different dataset of 16,231 females were analyzed for the effects of HC. Data were analyzed using various regression models. COC use was associated with higher vascular marker levels but lower chemokine levels. We found no association with progestin-only use. The association with differential cell counts was limited for most HC types. This study emphasizes the widespread impact of HC on the immune and vascular systems and how the impact may differ between HC types. The biomarker associations found could be targets for elucidation in functional studies.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No Sex Differences in the Amount of Type IV Collagen in Wistar Rats Regardless of Sampling Strategy Wistar大鼠体内IV型胶原蛋白含量无性别差异。

IF 2.2 4区医学

Apmis Pub Date : 2026-07-13 DOI: 10.1111/apm.70236

Patrik Mik, Katsiaryna Barannikava, Polina Surkova, Richard Pálek, Jáchym Rosendorf, Štěpánka Jansová, Václav Liška, Zbyněk Tonar

{"title":"No Sex Differences in the Amount of Type IV Collagen in Wistar Rats Regardless of Sampling Strategy","authors":"Patrik Mik, Katsiaryna Barannikava, Polina Surkova, Richard Pálek, Jáchym Rosendorf, Štěpánka Jansová, Václav Liška, Zbyněk Tonar","doi":"10.1111/apm.70236","DOIUrl":"10.1111/apm.70236","url":null,"abstract":"Serum collagen IV (Col IV) is an important marker for staging liver fibrosis, but histological baselines in healthy tissue are often lacking or biased. This study applied unbiased stereological sampling to quantify liver Col IV in healthy adult Wistar rats and assess sex differences. No significant differences were observed between sampling strategies (simple, systematic, or stratified random) or between males (4.26% ± 0.93%) and females (3.71% ± 1.27%), yielding a pooled mean of 3.96% ± 1.11%. The Col IV-positive area (3.62% ± 1.29%) did not differ significantly from the total collagen area measured by Sirius red (3.06% ± 1.72%), although a statistical trend was noted (R2 = 0.5845, p = 0.0767). These data indicate that IHC-based area measurements capture a larger structural footprint of the mesh-like Col IV network than biochemical mass-based methods, and that 58% of the variance in Col IV area is explained by Sirius red-positive area, suggesting a regulated spatial balance. The absence of sex effects on Col IV implies that previously reported sex-related differences in total liver collagen likely involve other collagen types. Unbiased Col IV quantification may support validation of minimally invasive methods for staging liver fibrosis.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Modified Extraperitoneal Laparoscopic Intrafascial Radical Prostatectomy in Prostate Cancer and Analysis of Factors Influencing Postoperative Urinary Continence Recovery 改良腹腔镜筋膜内前列腺根治术治疗前列腺癌的疗效及影响术后尿失禁恢复的因素分析。

IF 2.2 4区医学

Apmis Pub Date : 2026-07-09 DOI: 10.1111/apm.70233

Huan Cheng, Zjin Wan, Jingyang Liang, Haosen Lu, Yue Zhou, Lijun Mao

{"title":"Efficacy of Modified Extraperitoneal Laparoscopic Intrafascial Radical Prostatectomy in Prostate Cancer and Analysis of Factors Influencing Postoperative Urinary Continence Recovery","authors":"Huan Cheng, Zjin Wan, Jingyang Liang, Haosen Lu, Yue Zhou, Lijun Mao","doi":"10.1111/apm.70233","DOIUrl":"10.1111/apm.70233","url":null,"abstract":"<div>\u0000 \u0000 This study was designed to expound the efficacy of a modified extraperitoneal laparoscopic radical prostatectomy (ELRP) technique involving total intrafascial resection with the standard interfascial nerve-sparing technique in patients with prostate cancer (PC), and to identify factors influencing postoperative urinary continence recovery following the modified procedure. Phase 1: A retrospective analysis compared modified (n = 100) vs. standard ELRP (n = 100) for surgical outcomes, urodynamics, erectile function, and continence recovery. Phase 2: Analysis of 400 modified ELRP patients identified independent continence recovery risk factors via logistic regression (poor-continence: n = 63; good-continence: n = 337). At 6 months, the modified group showed greater urodynamic improvement (p < 0.05). Erectile function and continence were comparable at 1/3 months but better in the modified group at 6 months (p < 0.05). Age ≥ 65 years, BMI ≥ 24.0 kg/m2, TURP history, prostate volume ≥ 30 cm3, preserved urethral length ≤ 5 cm, and absence of regular pelvic floor exercises were associated with postoperative continence recovery in PC patients (p < 0.05). Modified ELRP provides favorable oncological and functional outcomes in PC patients. Several patient- and surgery-related factors were associated with postoperative urinary continence recovery.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Risk Human Papillomavirus Prevalence and Genotype Distribution in Two Opportunistic Cervical Screening Periods in Spain: A Tertiary Hospital-Based Study 高危人乳头瘤病毒流行和基因型分布在西班牙两次机会性宫颈筛查期：一项基于三级医院的研究。

IF 2.2 4区医学

Apmis Pub Date : 2026-07-07 DOI: 10.1111/apm.70234

Cristina Díaz del Arco, María Aguilera Hervás, Mar Ramírez Mena, Ricardo García Martínez, Dolores García López, Luis Ortega Medina, María Jesús Fernández Aceñero

{"title":"High-Risk Human Papillomavirus Prevalence and Genotype Distribution in Two Opportunistic Cervical Screening Periods in Spain: A Tertiary Hospital-Based Study","authors":"Cristina Díaz del Arco, María Aguilera Hervás, Mar Ramírez Mena, Ricardo García Martínez, Dolores García López, Luis Ortega Medina, María Jesús Fernández Aceñero","doi":"10.1111/apm.70234","DOIUrl":"10.1111/apm.70234","url":null,"abstract":"<div>\u0000 \u0000 High-risk human papillomavirus (HPV) infection is the main cause of cervical cancer and a central target of vaccination and screening programs. This study aimed to evaluate temporal changes in high-risk HPV prevalence and genotype distribution and their association with cytological and histological findings in an opportunistic screening population. Cervical cytology samples processed in 2013–2015 and 2022–2024 at a tertiary center in Spain were analyzed using the same high-risk HPV assay (n = 4724). Age-standardized prevalence and multivariable logistic regression models were applied, and birth cohort (≥ 1994 vs. < 1994) was used as an ecological proxy for vaccination exposure. After age standardization, high-risk HPV prevalence declined from 35.2% in 2013–2015 to 22.8% in 2022–2024, and screening in the later period was independently associated with lower odds of HPV infection (adjusted odds ratio 0.53). Most high-risk genotypes decreased over time, whereas HPV45 and HPV68 showed higher adjusted odds of detection. Coinfection prevalence declined. HPV positivity increased with cytological severity, and high-grade lesions clustered mainly around HPV16, HPV31, HPV52, HPV58 and HPV66. These findings highlight evolving patterns of high-risk HPV genotype distribution and support genotype-specific surveillance.\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Cell Composition and Prognosis in Node-Positive, Irradiated Breast Cancer Patients in the DBCG-IMN2 Study DBCG-IMN2研究中淋巴结阳性放疗乳腺癌患者的免疫细胞组成和预后

IF 2.2 4区医学

Apmis Pub Date : 2026-06-25 DOI: 10.1111/apm.70231

Demet Özcan, Anders Winther Mølby Nielsen, Patricia Switten Nielsen, Jan Alsner, Lise Bech Jellesmark Thorsen, Jens Overgaard, Birgitte Vrou Offersen, Trine Tramm

{"title":"Immune Cell Composition and Prognosis in Node-Positive, Irradiated Breast Cancer Patients in the DBCG-IMN2 Study","authors":"Demet Özcan, Anders Winther Mølby Nielsen, Patricia Switten Nielsen, Jan Alsner, Lise Bech Jellesmark Thorsen, Jens Overgaard, Birgitte Vrou Offersen, Trine Tramm","doi":"10.1111/apm.70231","DOIUrl":"https://doi.org/10.1111/apm.70231","url":null,"abstract":"High levels of tumor-infiltrating lymphocytes (TILs) are associated with improved survival after radiotherapy in breast cancer (BC) patients, particularly in estrogen receptor (ER)-negative disease. This study investigated the prognostic relevance of immune cell subsets in irradiated patients. Node-positive patients (N = 1307) from the Danish Breast Cancer Group internal mammary node (IMN)2 study were included, in which IMN irradiation (IMNI) was allocated by laterality. Tissue microarrays were stained with multiplex immunohistochemistry for CD8+, CD4+, and FOXP3+ T-cells, CD68+ macrophages, and CD11c+ dendritic cells. Digital image analysis quantified immune infiltration across spatial compartments. Prognostic associations with distant recurrence, breast cancer-specific mortality, and overall mortality (OM) were assessed using multivariable flexible parametric survival models, including ER-stratified analyses. T-cell infiltration demonstrated a strong inverse association with OM, with 12-year OM-risk decreasing from 42% in tumors with lowest CD8+ infiltration to 27% in those with highest (HR 0.52, 95% CI (0.36–0.76)). The association was strongest in the ER-negative subgroup. In ER-positive disease, prognostic benefit was observed at low-to-moderate infiltration. CD68+ and CD11c+ infiltration correlated with improved outcomes, but with weaker effects. Immune markers were not predictive of IMNI benefit. These findings demonstrate that T-cell subsets are strong prognostic markers in irradiated BC patients, especially in ER-negative disease.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary Secretory-IgA and IgG Responses and Inflammatory Markers in Patients With Pseudomonas aeruginosa Urinary Tract Infections 铜绿假单胞菌尿路感染患者尿分泌iga和IgG反应及炎症标志物

IF 2.2 4区医学

Apmis Pub Date : 2026-06-25 DOI: 10.1111/apm.70232

Ayse Corap Cakmak, Niels Høiby, Lars Christophersen, Franziska Schwartz, Magnus Bock, Claus Moser

{"title":"Urinary Secretory-IgA and IgG Responses and Inflammatory Markers in Patients With Pseudomonas aeruginosa Urinary Tract Infections","authors":"Ayse Corap Cakmak, Niels Høiby, Lars Christophersen, Franziska Schwartz, Magnus Bock, Claus Moser","doi":"10.1111/apm.70232","DOIUrl":"https://doi.org/10.1111/apm.70232","url":null,"abstract":"Recurrent UTIs (rUTIs) impair quality of life and contribute to antimicrobial resistance. Pseudomonas aeruginosa is particularly challenging due to intrinsic and acquired resistance. Identification of urinary biomarkers may improve understanding and diagnosis of localized and systemic UTIs. Urine anti-P. aeruginosa secretory IgA (s-IgA), IgG, and a selection of inflammatory markers were quantified using ELISA and Luminex. Urine samples with P. aeruginosa-positive cultures (n = 28), healthy controls (n = 23), culture-negative hospitalized patients (n = 25), and cultures positive for Escherichia coli or Enterococcus faecalis (n = 27) were included. Anti-P. aeruginosa s-IgA and IgG were highest in P. aeruginosa cases (median OD s-IgA 0.2040; IgG 0.4330) and exceeded healthy controls (s-IgA p < 0.001; IgG p < 0.0001). No difference was observed between single and rUTI, but s-IgA in multiple P. aeruginosa-positive rUTIs was increased versus healthy (p = 0.0034) and culture-negative controls (p = 0.0228). IL-8 (297.5 vs. 27.00 pg/mL, p = 0.002), lipocalin (32,440 vs. 6673 pg/mL, p = 0.0287), and IL-6 (11.0 vs. 6.5 pg/mL, p = 0.011) were elevated compared to healthy controls. Urinary s-IgA best reflected P. aeruginosa UTI status, while IL-8, lipocalin, and IL-6 may indicate UTI.","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70232","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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