Apmis最新文献 - Book学术

Unraveling the Transcriptomic Adaptations of Streptococcus mutans Biofilm to the Post-Biotic Impact of Lactiplantibacillus plantarum 揭示变形链球菌生物膜对植物乳杆菌生物后影响的转录组适应性

IF 2.2 4区医学

Apmis Pub Date : 2025-07-21 DOI: 10.1111/apm.70054

Aiswarya Sudheer, Zarin Taj, Ilathu Kandin Nidhin, Indranil Chattopadhyay

{"title":"Unraveling the Transcriptomic Adaptations of Streptococcus mutans Biofilm to the Post-Biotic Impact of Lactiplantibacillus plantarum","authors":"Aiswarya Sudheer, Zarin Taj, Ilathu Kandin Nidhin, Indranil Chattopadhyay","doi":"10.1111/apm.70054","DOIUrl":"https://doi.org/10.1111/apm.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is a multifactorial disease influenced by microbial dysbiosis and biofilm-induced chronic inflammation. <i>Streptococcus mutans</i>, a principal pathogen, aggravates OSCC by fostering an immunosuppressive tumor microenvironment via biofilm development and virulence-related metabolic alterations. This work investigated the post-biotic effects of <i>Lactiplantibacillus plantarum</i> in reducing <i>S</i>. <i>mutans</i>-related OSCC by obstructing bacterial adhesion, biofilm integrity, and virulence gene expression. GC–MS research revealed that the cell-free supernatant (CFS) of <i>L</i>. <i>plantarum</i> contains the bioactive metabolite 2,4-di-tert-butylphenol (DTP), which demonstrates significant antibacterial and anti-tumor activities. The new antimicrobial peptide Plpl_18 exhibited substantial biofilm inhibition and reduction of bacterial viability. Transcriptomic research indicated that <i>S</i>. <i>mutans</i> 890 treatment with DTP and Plpl_18 downregulated essential biofilm-associated genes (<i>gtfB</i>, <i>gtfC</i>), disturbed carbohydrate metabolism, and initiated a metabolic transition towards lactose utilization. Molecular docking and molecular dynamics simulations (MDS) validated persistent interactions between DTP and Plpl_18 with bacterial virulence factors and OSCC-related proteins (p38, NF-κB), underscoring their therapeutic potential. This research offers innovative perspectives on probiotic biofilm suppression methods and identifies DTP and Plpl_18 as potential options for targeted treatments against <i>S</i>. <i>mutans</i>-induced OSCC. Subsequent investigations into clinical applications may facilitate the development of novel antibacterial interventions and cancer treatment methodologies.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-145-5p Regulates Inflammatory Response via Targeting CD40 in Chronic Rhinosinusitis With Nasal Polyposis MiR-145-5p通过靶向CD40调控慢性鼻窦炎伴鼻息肉病的炎症反应

IF 2.2 4区医学

Apmis Pub Date : 2025-07-18 DOI: 10.1111/apm.70045

Lei Wang, Guangyao Wu, Xixia Li, Xiaoqin Liu, Xiaoyan Xu

{"title":"MiR-145-5p Regulates Inflammatory Response via Targeting CD40 in Chronic Rhinosinusitis With Nasal Polyposis","authors":"Lei Wang, Guangyao Wu, Xixia Li, Xiaoqin Liu, Xiaoyan Xu","doi":"10.1111/apm.70045","DOIUrl":"https://doi.org/10.1111/apm.70045","url":null,"abstract":"<div>\u0000 \u0000 <p>Chronic rhinosinusitis with nasal polyps (CRSwNP) lacks effective therapies, highlighting miRNAs as potential therapeutic targets owing to their biological functions and analytical accessibility. This study aimed to investigate the role of miR-145-5p in CRSwNP. Serum samples and inferior turbinate mucosa tissues were collected from 175 CRSwNP patients and 90 nasal septum deviation (NSD) subjects. qRT-PCR measured miR-145-5p expression in serum and tissue samples, and CD40 expression in tissues. ROC curve evaluated the diagnostic efficacy. Associations with disease severity and eosinophilic CRSwNP (ECRSwNP) risk were analyzed through correlation and logistic regression analysis. Dual-luciferase assay confirmed miR-145-5p targeting CD40. LPS-induced inflammation in human nasal epithelial cells (hNEpCs) assessed the miR-145-5p/CD40 axis's impact on IL-6, IL-1β, and TNF-α levels. Tissue and serum miR-145-5p effectively diagnosed CRSwNP. Reduced miR-145-5p correlated with disease severity and was an independent ECRSwNP risk factor. In LPS-stimulated hNEpCs, miR-145-5p overexpression suppressed IL-6, IL-1β, and TNF-α. CD40 expression inversely correlated with miR-145-5p and amplified inflammatory responses mitigated by miR-145-5p overexpression. MiR-145-5p, serving as a diagnostic biomarker for CRSwNP, was negatively correlated with disease severity. MiR-145-5p overexpression attenuated inflammation in hNEpCs by targeting CD40, thereby involving itself in the progression of CRSwNP, suggesting therapeutic potential for CRSwNP management.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling lncRNA H19的下调通过ATR/CHK1信号失活增强结直肠癌的放射敏感性

IF 2.2 4区医学

Apmis Pub Date : 2025-07-16 DOI: 10.1111/apm.70052

Yuting Liang, Wanlu Zhang, Ao Dungerile, Juping Wang, Runmei Wang

{"title":"Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling","authors":"Yuting Liang, Wanlu Zhang, Ao Dungerile, Juping Wang, Runmei Wang","doi":"10.1111/apm.70052","DOIUrl":"https://doi.org/10.1111/apm.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>Radiotherapy (RT) is commonly used in the treatment of colorectal cancer (CRC), but the resistance of cancer cells to radiation remains a significant challenge in clinical practice. This study aimed to investigate whether long non-coding RNA (lncRNA) H19 could affect the sensitivity of CRC cells to radiation. The levels of H19 in CRC tissues and adjacent normal tissues, as well as in radioresistant and radiosensitive CRC tissues, were evaluated using RT-qPCR. The levels of H19 were found to be higher in CRC tissues compared to adjacent normal tissues, as well as in radioresistant CRC tissues compared to radiosensitive tissues. Depletion of H19 intensified the anti-tumor effects of radiation in CRC cells in vivo through further decreasing proliferation and enhancing apoptosis. In an HCT116 subcutaneous xenograft model, downregulation of H19 enhanced the anti-tumor effect of radiation in CRC in vivo. Mechanistically, H19 was observed to interact with ATR. Meanwhile, H19 deficiency resulted in the suppression of ATR and CHK1 phosphorylation in CRC cells, suggesting that H19 knockdown could potentially impair DNA damage repair through ATR/CHK1 signaling. Collectively, downregulation of H19 could enhance the sensitivity of CRC cells to RT by impeding DNA damage repair through the inactivation of the ATR/CHK1 signaling pathway.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Nanomaterial Strategies for Antibacterial and Antibiofilm Applications: Innovations in Bacterial Detection, Mechanistic Understanding, and Therapeutic Interventions to Address Antibiotic Resistance 抗菌和抗生素膜应用的新兴纳米材料策略：细菌检测，机制理解和治疗干预的创新，以解决抗生素耐药性

IF 2.2 4区医学

Apmis Pub Date : 2025-07-16 DOI: 10.1111/apm.70050

Manoj Kumar Goshisht

{"title":"Emerging Nanomaterial Strategies for Antibacterial and Antibiofilm Applications: Innovations in Bacterial Detection, Mechanistic Understanding, and Therapeutic Interventions to Address Antibiotic Resistance","authors":"Manoj Kumar Goshisht","doi":"10.1111/apm.70050","DOIUrl":"https://doi.org/10.1111/apm.70050","url":null,"abstract":"<div>\u0000 \u0000 <p>The rampant increase in antimicrobial resistance and stagnant pipeline of antibiotic development is a global concern. Biofilms further make the infections more persistent and incurable by providing a protective environment to the microbes. It demands the development of innovative therapeutics without delay. Nanomaterial-based therapeutics is a promising approach to combat deadly bacterial infections. The distinct shape, size, and physical characteristics of nanomaterials (NMs) make them able to target biofilms through various mechanisms and thrash recalcitrant deadly infections. This comprehensive review describes (i) historical perspective of antibiotics and their role in combating microbes; (ii) different mechanisms through which bacteria develop resistance against antibiotics; (iii) different sensing mechanisms to detect bacteria; (iv) NMs to treat planktonic bacteria; (v) NMs to treat biofilms (both oral and wound biofilms); (vi) different types of NMs (e.g., metal NMs, polymeric NMs, carbon NMs) along with photothermal effects to combat microbes.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Activity of Antifungals in Combination With Meropenem or Daptomycin Against Dual Candida albicans–Bacterial Biofilms 美罗培南或达托霉素联合抗真菌药物对白色念珠菌-细菌双生物膜的体外活性研究

IF 2.2 4区医学

Apmis Pub Date : 2025-07-15 DOI: 10.1111/apm.70047

Agim Osmani, Sema Askın Keceli, Doganhan Kadir Er, Huseyin Uzuner, Feriha Ercan, Ayca Karagoz Koroğlu

{"title":"In Vitro Activity of Antifungals in Combination With Meropenem or Daptomycin Against Dual Candida albicans–Bacterial Biofilms","authors":"Agim Osmani, Sema Askın Keceli, Doganhan Kadir Er, Huseyin Uzuner, Feriha Ercan, Ayca Karagoz Koroğlu","doi":"10.1111/apm.70047","DOIUrl":"https://doi.org/10.1111/apm.70047","url":null,"abstract":"<p>The objective was to investigate the effectiveness of anidulafungin (ANI), voriconazole (VOR), and amphotericin (AMB) combined with meropenem against dual biofilms of <i>Candida albicans</i> and <i>Pseudomonas aeruginosa</i>, as well as with daptomycin against dual biofilms of <i>C. albicans</i>–<i>Staphylococcus epidermidis</i>. MIC values of antifungals were determined by CLSI M27-A3 method. The effectiveness of antifungals in combination with antibiotics against biofilms was tested by Checkerboard analysis. Biofilm quantifications were tested by the XTT method. The biofilm images were captured using SEM. The quantity of <i>C. albicans</i>–<i>S. epidermidis and C. albicans</i>–<i>P. aeruginosa</i> was lower (83.2% and 56.3%, respectively) than that of single Candida biofilm. The SMIC50 values for single <i>C. albicans</i> biofilms were 32-fold higher for AMB and VOR, and 128-fold higher for ANI when compared to dual Candida biofilms. Daptomycin combined with ANI or VOR showed antagonistic effects against <i>C. albicans</i>–<i>S. epidermidis</i> biofilms, while meropenem in combination with all antifungals was found to be additive against <i>C. albicans</i>–<i>P. aeruginosa</i> biofilms. Antifungals and their combinations resulted in a decrease in blastospore and bacterial density, as observed through SEM. In <i>C. albicans</i>–<i>P. aeruginosa</i> biofilms, using meropenem alongside antifungals would be beneficial due to its additive effect. Daptomycin combined with ANI or VOR may not be effective in <i>C. albicans</i>–<i>S. epidermidis</i> coinfections.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Anti-CMV TCR CDR3s via Blood and Tumor Exome Files Correlates With Increased Survival for Stomach Adenocarcinoma and Soft Tissue Sarcoma 通过血液和肿瘤外显子组文件检测抗cmv TCR CDR3s与胃腺癌和软组织肉瘤的生存率增加相关

IF 2.2 4区医学

Apmis Pub Date : 2025-07-13 DOI: 10.1111/apm.70053

Utsav Kapoor, Michael T. Aboujaoude, Konrad J. Cios, Andrea Chobrutskiy, Boris I. Chobrutskiy, George Blanck

{"title":"Detection of Anti-CMV TCR CDR3s via Blood and Tumor Exome Files Correlates With Increased Survival for Stomach Adenocarcinoma and Soft Tissue Sarcoma","authors":"Utsav Kapoor, Michael T. Aboujaoude, Konrad J. Cios, Andrea Chobrutskiy, Boris I. Chobrutskiy, George Blanck","doi":"10.1111/apm.70053","DOIUrl":"https://doi.org/10.1111/apm.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>While the role of anti-cytomegalovirus (CMV) T-cell responses in the cancer setting is becoming increasingly recognized, the association between CMV and survival outcomes has not been subject to study through more recent immunogenomic approaches. Thus for this study, we extracted T-cell receptor (TCR) recombination reads from whole exome sequencing (WXS) and RNA sequencing (RNAseq) files of stomach adenocarcinoma (STAD) and soft tissue sarcoma (SARC) samples from the Cancer Genome Atlas (TCGA) and matched the corresponding TCR complementarity determining region-3 (CDR3) amino acid (AA) sequences represented by these reads to known anti-CMV CDR3 sequences. Results indicated that the recovery of both TRA and TRB recombination reads from WXS files, from either blood or tumor samples, which corresponded to AA sequences matching anti-CMV CDR3s, correlated with better survival probabilities for both STAD and SARC. However, TCR CDR3s represented by recombination reads recovered from RNAseq files and matching anti-CMV TCR CDR3 AA sequences correlated with lower survival probabilities. These results raise the question of whether exposure to CMV prior to onset or progression of cancer may lead to better survival probability whereas a potentially active T-cell response to CMV during cancer onset or progression could represent an ongoing comorbidity? In conclusion, TCR CDR3s may be an important indicator of prognosis for stomach cancer and soft tissue sarcoma and may support further research into anti-CMV CDR3s as a tool for development of immunotherapy for these cancers.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Value of lncRNA NEAT1 in Patients With Hidradenitis Suppurativa lncRNA - NEAT1在化脓性汗腺炎中的临床价值

IF 2.2 4区医学

Apmis Pub Date : 2025-07-13 DOI: 10.1111/apm.70049

Li Zeng, Xiaoshuang Liao, Peng Guo, Xiaojiao Rong, Bo Yang, Jialin Zou

{"title":"Clinical Value of lncRNA NEAT1 in Patients With Hidradenitis Suppurativa","authors":"Li Zeng, Xiaoshuang Liao, Peng Guo, Xiaojiao Rong, Bo Yang, Jialin Zou","doi":"10.1111/apm.70049","DOIUrl":"https://doi.org/10.1111/apm.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>Hidradenitis suppurativa (HS) is characterized by chronic inflammation. LncRNA NEAT1 has been implicated in inflammatory skin conditions. This study aimed to investigate NEAT1 expression and its potential clinical implications in HS patients. A total of 182 individuals participated in this research, comprising 80 individuals in the control group and 102 HS patients in the HS group. QRT-PCR quantified the expression of NEAT1. Concentrations of inflammatory cytokines, including IL-1β, IL-6, IL-17, and TNF-α, were measured by ELISA. The association between NEAT1 level and these inflammatory markers was assessed by Pearson's correlation analysis. Multivariate logistic regression analysis was employed to estimate potential risk factors for HS. NEAT1 expression was elevated in HS patients compared to controls, with an area under the ROC curve measuring 0.8904, yielding a sensitivity of 69.61% and a specificity of 97.5%. Inflammatory factors IL-1β, IL-6, IL-17, and TNF-α were increased in HS patients and positively related to NEAT1 expression. The analysis identified smoking, ESR, CRP, and NEAT1 as risk factors for HS. In conclusion, increased NEAT1 in HS patients correlated with the disease severity. The expression level of NEAT1 was positively correlated with inflammatory factors, highlighting the role of smoking, ESR, CRP, and NEAT1 as key risk factors in HS.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association Analysis of White Blood Cell Trajectories and 30-Day Mortality in Ventilator-Associated Pneumonia Patients: A Cohort Study Based on the MIMIC-IV Database 呼吸机相关肺炎患者白细胞轨迹与30天死亡率的关联分析：基于MIMIC-IV数据库的队列研究

IF 2.2 4区医学

Apmis Pub Date : 2025-07-13 DOI: 10.1111/apm.70042

Jian Fang, Liang Liao

{"title":"Association Analysis of White Blood Cell Trajectories and 30-Day Mortality in Ventilator-Associated Pneumonia Patients: A Cohort Study Based on the MIMIC-IV Database","authors":"Jian Fang, Liang Liao","doi":"10.1111/apm.70042","DOIUrl":"https://doi.org/10.1111/apm.70042","url":null,"abstract":"<div>\u0000 \u0000 <p>Abnormal white blood cell (WBC) counts have been associated with ventilator-associated pneumonia (VAP). However, the prognostic value of WBC trajectory changes in VAP patients remains understudied. This study aimed to explore the correlation between WBC trajectory changes and the 30-day mortality rate in VAP patients. Data from VAP patients in the MIMIC-IV database were included. Group-based trajectory analysis was used to categorize patients based on their WBC patterns within 7 days of ICU admission. Cox proportional hazards regression analysis was conducted to assess the association between WBC trajectories and 30-day mortality. A total of 1194 eligible VAP patients were included. Five distinct WBC trajectories were identified. Compared to the trajectory group with continuously normal WBC levels (Class 5), the group with initially high levels but subsequent decrease (Class 2) and the group with continuously increasing WBC levels (Class 3) showed independent associations with increased mortality (OR > 1, <i>p</i> < 0.05). Additionally, restricted cubic splines (RCS) results indicated a possible linear relationship between WBC counts on the 6th and 7th days and the 30-day survival status of VAP patients. Within the first week of ICU admission, both the group with initially low levels but persistently increasing WBC trajectories and the group with initially high levels but continuously decreasing WBC trajectories were associated with increased 30-day mortality in VAP patients. This finding offers insights into predicting short-term mortality risks related to VAP and aids clinicians in early identification of high-risk patients.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of Actinomyces odontolyticus Pansinusitis in a Leflunomide-Treated Patient With Psoriatic Arthritis: A Rare Opportunistic Infection 在来氟米特治疗的银屑病关节炎患者中首次报道放线菌溶牙性全鼻窦炎：罕见的机会性感染

IF 2.2 4区医学

Apmis Pub Date : 2025-07-11 DOI: 10.1111/apm.70048

Angelo Nigro

引用次数: 0

miR-143 and miR-145 in Colorectal Cancer: A Digital Pathology Approach on Expressions and Protein Correlations miR-143和miR-145在结直肠癌中的表达和蛋白相关性的数字病理学方法

IF 2.2 4区医学

Apmis Pub Date : 2025-07-11 DOI: 10.1111/apm.70051

Erik Nesje Wiik, Henrik Sahlin Pettersen, Haakon Skogseth, Jostein Halgunset, Arne Wibe

{"title":"miR-143 and miR-145 in Colorectal Cancer: A Digital Pathology Approach on Expressions and Protein Correlations","authors":"Erik Nesje Wiik, Henrik Sahlin Pettersen, Haakon Skogseth, Jostein Halgunset, Arne Wibe","doi":"10.1111/apm.70051","DOIUrl":"https://doi.org/10.1111/apm.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>miR-143 and miR-145 have been reported as downregulated in colorectal cancer (CRC) compared to normal mucosa, with regulatory effects on proteins involved in carcinogenesis. These findings primarily derive from tissue homogenate analyses and experimental models. The present study employs in situ methodology to reassess miR-143 and miR-145 expression in CRC and their associations with validated protein targets within the native tumor microenvironment. Expression patterns of miR-143, miR-145, and eight previously experimentally validated target proteins were analyzed in clinical samples from 100 CRC patients using in situ hybridization, immunohistochemistry, and deep learning-based epithelial segmentation. Expression levels of miR-143 and miR-145 showed no significant difference between CRC and normal mucosa, though considerable inter-patient variability was observed. Among 11 examined miRNA-protein relationships, only four showed significant correlations, exhibiting positive associations that contrast with previously reported inverse relationships. Subgroup analyses revealed no statistically significant association between miRNA expression variability and examined clinicopathological parameters. These findings highlight the importance of in situ validation for results obtained from tissue homogenates and in vitro experiments. Additional research is warranted to determine the prognostic significance of miR-143 and miR-145 in clinical outcomes.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0