Apmis最新文献

{"title":"First Report of Actinomyces odontolyticus Pansinusitis in a Leflunomide-Treated Patient With Psoriatic Arthritis: A Rare Opportunistic Infection","authors":"Angelo Nigro","doi":"10.1111/apm.70048","DOIUrl":"https://doi.org/10.1111/apm.70048","url":null,"abstract":"","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-143 and miR-145 in Colorectal Cancer: A Digital Pathology Approach on Expressions and Protein Correlations","authors":"Erik Nesje Wiik,&nbsp;Henrik Sahlin Pettersen,&nbsp;Haakon Skogseth,&nbsp;Jostein Halgunset,&nbsp;Arne Wibe","doi":"10.1111/apm.70051","DOIUrl":"https://doi.org/10.1111/apm.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>miR-143 and miR-145 have been reported as downregulated in colorectal cancer (CRC) compared to normal mucosa, with regulatory effects on proteins involved in carcinogenesis. These findings primarily derive from tissue homogenate analyses and experimental models. The present study employs in situ methodology to reassess miR-143 and miR-145 expression in CRC and their associations with validated protein targets within the native tumor microenvironment. Expression patterns of miR-143, miR-145, and eight previously experimentally validated target proteins were analyzed in clinical samples from 100 CRC patients using in situ hybridization, immunohistochemistry, and deep learning-based epithelial segmentation. Expression levels of miR-143 and miR-145 showed no significant difference between CRC and normal mucosa, though considerable inter-patient variability was observed. Among 11 examined miRNA-protein relationships, only four showed significant correlations, exhibiting positive associations that contrast with previously reported inverse relationships. Subgroup analyses revealed no statistically significant association between miRNA expression variability and examined clinicopathological parameters. These findings highlight the importance of in situ validation for results obtained from tissue homogenates and in vitro experiments. Additional research is warranted to determine the prognostic significance of miR-143 and miR-145 in clinical outcomes.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Characterization of Gum Kondagogu Stabilized Zinc Oxide Nanoparticles and Its Application as an Antibacterial Agent Against Wastewaterborne Bacteria","authors":"Aruna Jyothi Kora,&nbsp;Venkata Balarama Krishna Mullapudi","doi":"10.1111/apm.70046","DOIUrl":"https://doi.org/10.1111/apm.70046","url":null,"abstract":"<p>Wastewater recycling is one of the viable options for attaining sustainable water management. In this scenario, gum stabilized zinc oxide nanoparticles (ZnO NP) were synthesized employing the alkaline precipitation method with gum kondagogu stabilizer. Synthesized NP were characterized using UV–vis, DLS, zeta potential, XRD, FTIR, and TEM. ZnO NP exhibited an absorption peak at 351 nm in UV–vis, a zeta potential value of–35 mV, and a <i>z</i>-average value of 254 nm in DLS. XRD pattern showed a characteristic hexagonal wurtzite crystal structure and FTIR data indicated the capping of NP by various gum functional groups. The size of spherical NP varied from 12.4 to 35.7 nm, and the mean particle size was 24.8 ± 5.1 nm. The in vitro antibacterial activity of ZnO NP against wastewaterborne bacteria, <i>Escherichia coli</i> (Gram- negative) and <i>Bacillus subtilis</i> (Gram-positive) was studied with the resazurin broth assay. MIC values of 900 μg/mL and 900 μg/mL and MBC values of 1800 μg/mL and 900 μg/mL of ZnO NP were recorded towards <i>E. coli</i> and <i>B. subtilis</i>. Notably, ZnO NP exhibited superior bactericidal activity against <i>B. subtilis</i> compared to previous studies. Thus, the current study on ZnO NP mediated bacterial disinfection has wide applications in wastewater treatment and remediation of microbially contaminated effluents.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials and Candida auris: A Systematic Review of Emerging Strategies","authors":"Sergio A. Alanís-Ríos,&nbsp;Celia N. Sánchez-Domínguez,&nbsp;Hiram Villanueva-Lozano,&nbsp;Neri A. Álvarez Villalobos,&nbsp;Rogelio de J. Treviño-Rangel","doi":"10.1111/apm.70044","DOIUrl":"https://doi.org/10.1111/apm.70044","url":null,"abstract":"<div>\u0000 \u0000 <p>The alarming rise of multidrug-resistant <i>Candida auris</i> infections and the consequent scarcity of effective therapeutic options have spurred research into alternative strategies for their prevention and management. Nanomaterials have emerged as a promising avenue for the development of innovative antifungal approaches. A systematic search of MEDLINE, EMBASE, Web of Science, and Scopus databases up to January 3, 2024, was conducted to identify studies reporting on the antifungal efficacy of nanoparticles (NPs) against <i>C. auris</i>. This search included studies on metallic NPs, lipidic carriers, the use of chitosan as a matrix, and other nanoparticle synthesis methodologies, including green chemistry approaches. Of the 266 articles initially retrieved, 30 met the eligibility criteria. Metallic NPs, particularly silver nanoparticles, were the most extensively studied, whereas lipidic carriers were the least explored. All nanomaterials demonstrated antifungal activity, showing inhibition of fungal growth, biofilm disruption, reduced fungal burden, and significantly improved survival rates in animal models of <i>C. auris</i> candidemia. Ongoing research into innovative treatment and prevention strategies remains urgently needed to combat this growing public health threat.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of miR-222-3p Alleviates Acute Pancreatitis by Negatively Regulating the Expression of SIRT1","authors":"Tao Zhang,&nbsp;Jing Xiao,&nbsp;Wenzhao Chen","doi":"10.1111/apm.70043","DOIUrl":"https://doi.org/10.1111/apm.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of this study is to explore the correlation between the levels of miR-222-3p and the onset of acute pancreatitis (AP) and further verify the role of miR-222-3p in the pathogenesis of AP through targeted regulation of Sirtuin 1 (SIRT1). This study encompassed a total of 160 AP patients, including 80 patients with mild and moderate AP (non-SAP) and 80 patients with severe AP (SAP). The levels of miR-222-3p and SIRT1 were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) technology, and the potential significance of miR-222-3p in the diagnosis of AP was evaluated by receiver operating characteristic curve. The proliferation activity of cerulein-induced HPDE6-C7 cells was investigated by Cell Counting Kit-8 method. Meanwhile, the changes in the levels of inflammatory factors were quantified using enzyme-linked immunosorbent assay (ELISA) kits. The result showed that miR-222-3p was elevated in AP patients, especially in SAP patients. Elevated miR-222-3p levels showed diagnostic significance for AP. Inhibiting miR-222-3p promoted the proliferation of cerulein-damaged HPDE6-C7 cells and reduced inflammatory factor increases induced by cerulein. SIRT1 inhibition reversed the effects of miR-222-3p on cerulein-induced HPDE6-C7 cells. Inhibition of miR-222-3p may alleviate the development of AP by targeting SIRT1.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background","authors":"Bo Söderquist,&nbsp;Peter Wildeman,&nbsp;Marc Stegger,&nbsp;Bianca Stenmark","doi":"10.1111/apm.70038","DOIUrl":"https://doi.org/10.1111/apm.70038","url":null,"abstract":"<p>Hematogenous prosthetic joint infections (PJIs) are primarily associated with <i>Staphylococcus aureus</i>, and there is a 30%–40% risk of contracting a hematogenous PJI following an <i>S. aureus</i> bacteremia. The aim of this study was to investigate whether identical strains of <i>S. aureus</i> were present in each patient from a cohort with both bacteremia and PJI and to explore the genomic differences between paired isolates obtained from blood cultures and tissue biopsies. All patients with a PJI and a temporally concomitant bacteremia due to <i>S. aureus</i> from 2005 to 2020 were included. Paired isolates of <i>S. aureus</i> from tissue biopsies and blood cultures were subjected to whole-genome sequencing. Twenty-four episodes of PJI were identified in 23 patients. All pairwise isolates from individual patients belonged to the same multilocus sequence type, clonal complex, and core genome multilocus sequence typing (cgMLST) complex type. The median number of single nucleotide polymorphisms (SNPs) in the conserved core genomes between the pairwise isolates was 3. In conclusion, identical cgMLST complex types and low levels of SNP differences between paired isolates of <i>S. aureus</i> from blood cultures and tissue biopsies suggest hematogenous seeding in all cases of PJI in this cohort.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining the Role of Virulence Proteins From Pathogenic Anaerobes in the Initiation of Colorectal Cancer","authors":"Vinoj Gopalakrishnan,&nbsp;Vaijayanthi Saravanan,&nbsp;Maria Infant Majula Shifani Mahendran,&nbsp;Vinoth Boopathy,&nbsp;Rajan Vaithianathan,&nbsp;Sowmya Srinivasan,&nbsp;Srikrishna Krishnamurthy","doi":"10.1111/apm.70041","DOIUrl":"https://doi.org/10.1111/apm.70041","url":null,"abstract":"<div>\u0000 \u0000 <p>Colorectal cancer (CRC) is a rising threat in modern populations, driven by reduced dietary fiber intake, pollution, poor hygiene, and especially the overuse of antibiotics. Anaerobe-induced CRC has emerged as a focal area in current research, with particular attention to anaerobic pathogenic bacteria such as <i>Helicobacter pylori</i>, <i>Fusobacterium nucleatum</i>, <i>Solobacterium moorei</i>, <i>Bacteroides fragilis</i> toxin (BFT), <i>Peptostreptococcus anaerobius</i>, and <i>Parvimonas micra</i>. Pathogens inhabit concerning niche within the gut, releasing virulence factors that disrupt gut microbiota homeostasis, leading to significant dysbiosis and chronic inflammation. Persistent inflammatory state activates various inflammatory markers, triggering cancer-associated signaling pathways. Moreover, bacterial toxins and biofilm formation exacerbate these effects by activating multiple malignant signaling pathways. Review explores recent advances in therapeutic strategies, emphasizing the importance of a deeper understanding of anaerobes and their pivotal roles in CRC progression. We highlight the major anaerobes likely to be classified as grade I carcinogens in the future and underscore the critical need for effective interventions to mitigate their impact on CRC development.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting Therapeutic Response to Molecular Hydrogen in Autoimmune Diseases via Immunophenotyping","authors":"Shan-Wen Lui,&nbsp;Ting-Yu Hsieh,&nbsp;Jeng-Wei Lu,&nbsp;Yi-Jung Ho,&nbsp;Feng-Cheng Liu","doi":"10.1111/apm.70040","DOIUrl":"https://doi.org/10.1111/apm.70040","url":null,"abstract":"<div>\u0000 \u0000 <p>Autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), are characterized by immune dysregulation that leads to chronic inflammation and organ damage. Current therapeutic strategies—including corticosteroids, immunosuppressants, and biologics—often exhibit variable efficacy and are associated with potential adverse effects. Molecular hydrogen, recognized for its ability to scavenge mitochondrial reactive oxygen species and inhibit the NLRP3 inflammasome, has emerged as a promising adjunctive treatment. However, its immunomodulatory effects remain insufficiently defined. This study aimed to evaluate the immunological effects of molecular hydrogen-assisted therapy (MHAT) on immune cell subsets and to identify potential predictive biomarkers of treatment efficacy. A total of 25 patients with autoimmune diseases who received MHAT were included (RA: <i>n</i> = 14; SLE: <i>n</i> = 7; others: <i>n</i> = 4, including one each with psoriatic arthritis, primary Sjögren's syndrome, immune-related interstitial lung disease, and diffuse idiopathic skeletal hyperostosis). An additional 15 untreated RA patients served as controls for the assessment of MHAT-induced changes in lymphocyte profiles and type 1 regulatory T (Tr1) cells. MHAT was administered orally at a daily dose of 170 mg hydrogen-enriched coral calcium for three months. Immune phenotyping of T cells, B cells, and regulatory T cells was performed using flow cytometry before and after treatment. Among the 108 immune subsets analyzed, 15 exhibited significant changes, including 11 T cell and 4 B cell subsets. Disease-specific immune modulation was observed in RA patients, particularly characterized by increased proportions of programmed cell death protein 1 (PD-1⁺) T cells and Fas⁺ B cells, and a marked reduction in Tr1 cells compared to patients with SLE or other autoimmune diseases. Based on baseline immune profiles and the percent change in fatigue scores (assessed by the Brief Fatigue Inventory, BFI-T), a Hydrogen-assisted Treatment Response Prediction Index (HRPI) was developed, demonstrating strong predictive performance (ROC = 0.9375, <i>p</i> = 0.0118). HRPI values below −0.3 predicted favorable clinical responses, whereas values near zero were associated with poor outcomes. HRPI shows potential as a predictive biomarker for MHAT efficacy and guides personalized autoimmune treatment.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Account of a Research Expedition to Greenland in 1908 by Marie and August Krogh—Early 20th Century Experimental Physiology in the Field","authors":"Adam Bencard","doi":"10.1111/apm.70032","DOIUrl":"https://doi.org/10.1111/apm.70032","url":null,"abstract":"","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum miR-181a-5p Serves as a Diagnostic Biomarker for Sepsis and Predicts Clinical Outcomes","authors":"Huibo Li,&nbsp;Geng Yuan,&nbsp;Gang Luo,&nbsp;Xiangzhe Ni,&nbsp;Shuang Wu,&nbsp;Haiyun Chu,&nbsp;Ying Xiong","doi":"10.1111/apm.70037","DOIUrl":"https://doi.org/10.1111/apm.70037","url":null,"abstract":"<div>\u0000 \u0000 <p>MicroRNA (miRNA) shows promise as a biomarker for sepsis diagnosis and prognosis. In the present investigation, we examined the potential of miR-181a-5p as a clinical diagnostic and prognostic indicator for sepsis. This study enrolled 119 sepsis patients and 125 controls. Serum miR-181a-5p levels were assessed via RT-qPCR, revealing a significant upregulation in sepsis patients. The receiver operating characteristic (ROC) curve indicated miR-181a-5p could distinguish sepsis patients with 73.11% sensitivity and 89.60% specificity (AUC = 0.879). Pearson's analysis showed serum miR-181a-5p positively correlated with disease severity scores (APACHE II and SOFA), procalcitonin (PCT), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), malondialdehyde (MDA), and negative with glutathione peroxidase (GSH-Px). Kaplan–Meier survival analysis revealed that elevated serum miR-181a-5p levels were associated with a worse 28-day prognosis in sepsis patients (log-rank <i>p</i> = 0.022). In summary, this study identifies serum miR-181a-5p as a diagnostic and prognostic biomarker for sepsis, significantly linked to disease severity, inflammation, and oxidative stress, providing new avenues for sepsis management.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}