IF 2.6 4区医学

Apmis Pub Date : 2025-08-15 DOI: 10.1111/apm.70060

Zhang Zhao, Fadian Ding, Zhibo Zhang

{"title":"Epigenetic Silencing of SFRP5 Promotes Hepatocellular Carcinoma Progression and Metastasis via Wnt/β-Catenin Signaling","authors":"Zhang Zhao, Fadian Ding, Zhibo Zhang","doi":"10.1111/apm.70060","DOIUrl":"https://doi.org/10.1111/apm.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>Hepatocellular carcinoma (HCC) is an aggressive malignancy with poor prognosis, frequent metastasis, and therapy resistance. Epithelial-mesenchymal transition (EMT) and aberrant Wnt/β-catenin signaling are key drivers of HCC progression. Secreted frizzled-related protein 5 (SFRP5), a Wnt/β-catenin signaling antagonist, has been implicated in various cancers, but its role in HCC remains unclear. This study explores the regulatory interactions between SFRP5, EMT, and Wnt/β-catenin signaling in HCC. Bioinformatics analysis, patient-derived tissue samples, and in vitro experiments revealed significant downregulation of SFRP5 due to promoter hypermethylation. Methylation-specific PCR confirmed extensive SFRP5 methylation, while treatment with 5-Aza-2′-deoxycytidinerestored SFRP5 expression, suppressing Wnt/β-catenin signaling and EMT. Functional assays demonstrated that SFRP5 overexpression inhibited HCC cell proliferation, migration, and colony formation while promoting apoptosis. Western blot and immunofluorescence confirmed that SFRP5 restoration suppressed β-catenin and its targets (MYC, Cyclin D1, Survivin), increased E-cadherin, and decreased mesenchymal markers (Vimentin, Fibronectin, Twist). In vivo xenograft models showed that SFRP5 overexpression reduced tumor growth and EMT marker expression. These findings highlight SFRP5 as a tumor suppressor in HCC, where epigenetic silencing promotes tumor progression via Wnt/β-catenin signaling activation. Targeting SFRP5 methylation may provide a novel therapeutic strategy for HCC.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144843565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Subcarinal Angle on Parenchymal Involvement and Disease Severity in COVID-19 Pneumonia: A Retrospective Analysis 隆下角对COVID-19肺炎实质受累及病情严重程度影响的回顾性分析

IF 2.6 4区医学

Apmis Pub Date : 2025-08-13 DOI: 10.1111/apm.70062

Seda Akyol, Önder Eraslan

{"title":"Impact of Subcarinal Angle on Parenchymal Involvement and Disease Severity in COVID-19 Pneumonia: A Retrospective Analysis","authors":"Seda Akyol, Önder Eraslan","doi":"10.1111/apm.70062","DOIUrl":"https://doi.org/10.1111/apm.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aims to examine the relationship between the subcarinal angle (SCA) and the risk and severity of parenchymal involvement in COVID-19 positive patients. This retrospective study involved 2006 patients, categorized into a study group of 1003 COVID-19 positive patients with parenchymal involvement and a control group of 1003 patients with COVID-19-like symptoms but without parenchymal involvement. Receiver Operating Characteristic (ROC) analysis was used to establish cut-off values for SCA and age that predict disease progression and severity. The study and control groups had mean ages of 51.80 and 45.76 years, respectively. Males had a higher frequency of parenchymal involvement (<i>p</i> = 0.003). ROC analysis identified SCA cut-offs of 34.5°–35.5° for the right SCA, 39.5°–40.5° for the left SCA, and 74.5°–75.5° for the total SCA. Age cut-offs were set at 47 years for increased risk and 54 years for greater severity of involvement. SCA values above 36° for the right, 42° for the left, and 77° for the total significantly increased the Tomographic Severity Score (TSS), indicating more severe disease. The TSS was higher in males and positively correlated with age and SCA, suggesting that both factors are important in predicting the severity of COVID-19 pneumonia. SCA is a key predictor of the severity and extent of COVID-19 pneumonia. Measuring SCA alongside age can enhance early risk assessment, disease management, and the implementation of timely and effective interventions, potentially reducing the morbidity and mortality associated with the disease.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Human Neutrophil Response to Different Morphotypes of Candida albicans Compared to Bacterial Pathogens 早期人类中性粒细胞对不同形态白色念珠菌与细菌病原体的反应

IF 2.6 4区医学

Apmis Pub Date : 2025-08-12 DOI: 10.1111/apm.70059

Gizem Babuccu, Özgür Albayrak, Füsun Can, Özlem Doğan

{"title":"Early Human Neutrophil Response to Different Morphotypes of Candida albicans Compared to Bacterial Pathogens","authors":"Gizem Babuccu, Özgür Albayrak, Füsun Can, Özlem Doğan","doi":"10.1111/apm.70059","DOIUrl":"https://doi.org/10.1111/apm.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Neutrophils are among the first immune cells recruited during the critical early phase of infection; yet their reaction to <i>Candida albicans</i> morphotypes is not fully defined. Here, we aimed to investigate early neutrophil responses to <i>C. albicans</i> morphotypes compared to bacteria. Freshly isolated human neutrophils were incubated with heat-killed <i>C. albicans</i>, live <i>C. albicans</i>, <i>Escherichia coli</i>, and <i>Staphylococcus aureus</i> as bacterial comparisons for 30 min, 1 h, and 4 h. Afterward, neutrophil activation was assessed via gene expression of neutrophil elastase (ELANE) and myeloperoxidase (MPO), CD66b/CD11b surface marker expression, microbicidal activity, and cytokine release, including IL-22, IL-17A, IL-1ɑ, IL-1β, TNF-ɑ, and IL-6. Neutrophil response pathways were significantly more activated by hyphal <i>C. albicans</i> than HK <i>C. albicans, E</i><i>.coli</i><i>,</i> and <i>S. aureus</i>, including the highest MPO and ELANE expression within the first 4 h, compared to heat-killed <i>C. albicans</i> and bacteria. CD66b expression significantly upregulated at 1 h in response to hyphal <i>C. albicans</i>. Neutrophils exhibited a 68% candidacidal effect at 1 h. IL-22 peaked at 1 h and remained elevated; heat-killed <i>C. albicans</i> induced IL-22 and IL-17A at 4 h. Neutrophils show stronger early responses against <i>C. albicans</i> morphotypes compared to <i>E. coli</i> and <i>S. aureus</i>, particularly within 1 h.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Clarithromycin Combined With Oxymetazoline Hydrochloride Spray on Olfactory Function and Inflammatory Factors in Patients With Acute Exacerbations of Chronic Rhinosinusitis 克拉霉素联合盐酸羟美唑啉喷雾剂对慢性鼻窦炎急性加重期患者嗅觉功能及炎症因子的影响

IF 2.6 4区医学

Apmis Pub Date : 2025-08-05 DOI: 10.1111/apm.70055

Kailin Jin, Qin Zhao, Yinzhong Chen

{"title":"Effect of Clarithromycin Combined With Oxymetazoline Hydrochloride Spray on Olfactory Function and Inflammatory Factors in Patients With Acute Exacerbations of Chronic Rhinosinusitis","authors":"Kailin Jin, Qin Zhao, Yinzhong Chen","doi":"10.1111/apm.70055","DOIUrl":"https://doi.org/10.1111/apm.70055","url":null,"abstract":"<div>\u0000 \u0000 <p>This study is intended to expound the effect of clarithromycin combined with oxymetazoline hydrochloride (OZH) spray in acute exacerbations of chronic rhinosinusitis (AECRS). Patients with AECRS were retrospectively collected and divided into a control group and an observation group. The control group received clarithromycin, and the observation group received OZH spray in addition to clarithromycin. Symptom resolution time, nasal mucociliary transmission velocity, nasal endoscopy score, olfactory function, serum inflammatory factor levels, incidence of adverse reactions, and 6-month recurrence were recorded. After treatment, the observation group showed shorter resolution times for headache, runny nose, nasal congestion, and nasal mucosal edema; higher nasal mucociliary transport rate (MTR) and clearance (MCC); lower Lund-Kennedy score; more Grades I cases; fewer Grades III cases; lower levels of serum IL-6, TNF-α, hs-CRP, IL-8, and IL-1β; and lower recurrence rate than the control group (<i>p <</i> 0.05). The incidence of adverse reactions in the two groups was not statistically significant (<i>p</i> > 0.05). Clarithromycin combined with OZH spray is more effective in patients with AECRS, which can shorten symptom resolution time, increase nasal MTR, improve olfactory function, reduce inflammation and recurrence rates, and have a high degree of safety.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arthritis in Cystic Fibrosis—Comparison of a Single-Center Cohort and Published Case Reports/Series and a Review of the Literature 囊性纤维化关节炎：单中心队列与已发表病例报告/系列的比较及文献综述

IF 2.6 4区医学

Apmis Pub Date : 2025-08-05 DOI: 10.1111/apm.70058

Anne Sofie Rosenborg Peretz, Thomas Bryrup, Daniel Faurholt-Jepsen, Christoffer Tandrup Holst Nielsen

{"title":"Arthritis in Cystic Fibrosis—Comparison of a Single-Center Cohort and Published Case Reports/Series and a Review of the Literature","authors":"Anne Sofie Rosenborg Peretz, Thomas Bryrup, Daniel Faurholt-Jepsen, Christoffer Tandrup Holst Nielsen","doi":"10.1111/apm.70058","DOIUrl":"https://doi.org/10.1111/apm.70058","url":null,"abstract":"<p>Cystic fibrosis (CF) is commonly associated with musculoskeletal issues including inflammatory arthritis, CF arthritis. We present a retrospective cohort study which aims to describe the clinical characteristics, prevalence, and demographic associations of CF arthritis using both a clinical and literature cohort. We identified adult CF patients (≥ 18 years) with arthritis from the rheumatology clinic at Rigshospitalet (2020–2022). The clinical cohort (CC) was reviewed through electronic medical records. Literature cases were identified by searching online databases for relevant studies, case reports, and reviews on CF arthritis. Eleven CF patients with arthritis were identified (CC) from our clinic and 54 cases from the literature (literature cohort, LC). Both cohorts showed equal gender distribution. In the LC, arthritis onset had a median age of 11 years (range 2–28), while in the CC it was 26 years (range 13–43). Clinical features were similar in both cohorts: the majority had episodic relapsing arthritis, with two-thirds having mono-/oligoarthritis and one-third polyarthritis. Large joints were most commonly affected. No clear link to pulmonary disease or serologic markers was found. Immunosuppressive treatment was safe. CF arthritis is a heterogeneous condition, presenting as non-erosive, episodic oligo- or polyarthritis affecting both large and small joints.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum TP53TG1 Serves as a Diagnostic Biomarker for Sepsis and Has a Predictive Value for Clinical Outcomes 血清TP53TG1可作为败血症的诊断性生物标志物，对临床结果具有预测价值

IF 2.6 4区医学

Apmis Pub Date : 2025-08-04 DOI: 10.1111/apm.70057

Shuai Li, Tao Ma, Na Ye, Donglin Chen

{"title":"Serum TP53TG1 Serves as a Diagnostic Biomarker for Sepsis and Has a Predictive Value for Clinical Outcomes","authors":"Shuai Li, Tao Ma, Na Ye, Donglin Chen","doi":"10.1111/apm.70057","DOIUrl":"https://doi.org/10.1111/apm.70057","url":null,"abstract":"<div>\u0000 \u0000 <p>To investigate the diagnostic value of the lncRNA TP53TG1 in sepsis and its ability to predict clinical outcomes, 121 sepsis patients and 115 healthy controls were enrolled. RT-qPCR measured TP53TG1 expression, and the ROC curve assessed its diagnostic value. Pearson correlation analyzed correlations between TP53TG1 and inflammatory factors, APACHE II score, and SOFA score. Cox regression assessed the risk factors for 28-day mortality, and Kaplan–Meier survival analysis evaluated the effect of TP53TG1 levels on mortality in patients. TP53TG1 was downregulated in patients, with 79.34% sensitivity and 80.00% specificity for differentiating patients from healthy controls. The TP53TG1 level was negatively correlated with PCT, CRP, IL-6, TNF-α, WBC, Scr, AST, PT, lactate, the APACHE II score, and the SOFA score, but positively correlated with ALB. Additionally, TP53TG1 and the SOFA score were identified as risk factors influencing the 28-day survival of patients, and a decrease in TP53TG1 expression was significantly associated with reduced survival rates. This study showed that TP53TG1 can serve as a biomarker for the diagnosis of sepsis and is closely associated with disease severity and inflammatory responses.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Transcriptomic Adaptations of Streptococcus mutans Biofilm to the Post-Biotic Impact of Lactiplantibacillus plantarum 揭示变形链球菌生物膜对植物乳杆菌生物后影响的转录组适应性

IF 2.2 4区医学

Apmis Pub Date : 2025-07-21 DOI: 10.1111/apm.70054

Aiswarya Sudheer, Zarin Taj, Ilathu Kandin Nidhin, Indranil Chattopadhyay

{"title":"Unraveling the Transcriptomic Adaptations of Streptococcus mutans Biofilm to the Post-Biotic Impact of Lactiplantibacillus plantarum","authors":"Aiswarya Sudheer, Zarin Taj, Ilathu Kandin Nidhin, Indranil Chattopadhyay","doi":"10.1111/apm.70054","DOIUrl":"https://doi.org/10.1111/apm.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is a multifactorial disease influenced by microbial dysbiosis and biofilm-induced chronic inflammation. <i>Streptococcus mutans</i>, a principal pathogen, aggravates OSCC by fostering an immunosuppressive tumor microenvironment via biofilm development and virulence-related metabolic alterations. This work investigated the post-biotic effects of <i>Lactiplantibacillus plantarum</i> in reducing <i>S</i>. <i>mutans</i>-related OSCC by obstructing bacterial adhesion, biofilm integrity, and virulence gene expression. GC–MS research revealed that the cell-free supernatant (CFS) of <i>L</i>. <i>plantarum</i> contains the bioactive metabolite 2,4-di-tert-butylphenol (DTP), which demonstrates significant antibacterial and anti-tumor activities. The new antimicrobial peptide Plpl_18 exhibited substantial biofilm inhibition and reduction of bacterial viability. Transcriptomic research indicated that <i>S</i>. <i>mutans</i> 890 treatment with DTP and Plpl_18 downregulated essential biofilm-associated genes (<i>gtfB</i>, <i>gtfC</i>), disturbed carbohydrate metabolism, and initiated a metabolic transition towards lactose utilization. Molecular docking and molecular dynamics simulations (MDS) validated persistent interactions between DTP and Plpl_18 with bacterial virulence factors and OSCC-related proteins (p38, NF-κB), underscoring their therapeutic potential. This research offers innovative perspectives on probiotic biofilm suppression methods and identifies DTP and Plpl_18 as potential options for targeted treatments against <i>S</i>. <i>mutans</i>-induced OSCC. Subsequent investigations into clinical applications may facilitate the development of novel antibacterial interventions and cancer treatment methodologies.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-145-5p Regulates Inflammatory Response via Targeting CD40 in Chronic Rhinosinusitis With Nasal Polyposis MiR-145-5p通过靶向CD40调控慢性鼻窦炎伴鼻息肉病的炎症反应

IF 2.2 4区医学

Apmis Pub Date : 2025-07-18 DOI: 10.1111/apm.70045

Lei Wang, Guangyao Wu, Xixia Li, Xiaoqin Liu, Xiaoyan Xu

{"title":"MiR-145-5p Regulates Inflammatory Response via Targeting CD40 in Chronic Rhinosinusitis With Nasal Polyposis","authors":"Lei Wang, Guangyao Wu, Xixia Li, Xiaoqin Liu, Xiaoyan Xu","doi":"10.1111/apm.70045","DOIUrl":"https://doi.org/10.1111/apm.70045","url":null,"abstract":"<div>\u0000 \u0000 <p>Chronic rhinosinusitis with nasal polyps (CRSwNP) lacks effective therapies, highlighting miRNAs as potential therapeutic targets owing to their biological functions and analytical accessibility. This study aimed to investigate the role of miR-145-5p in CRSwNP. Serum samples and inferior turbinate mucosa tissues were collected from 175 CRSwNP patients and 90 nasal septum deviation (NSD) subjects. qRT-PCR measured miR-145-5p expression in serum and tissue samples, and CD40 expression in tissues. ROC curve evaluated the diagnostic efficacy. Associations with disease severity and eosinophilic CRSwNP (ECRSwNP) risk were analyzed through correlation and logistic regression analysis. Dual-luciferase assay confirmed miR-145-5p targeting CD40. LPS-induced inflammation in human nasal epithelial cells (hNEpCs) assessed the miR-145-5p/CD40 axis's impact on IL-6, IL-1β, and TNF-α levels. Tissue and serum miR-145-5p effectively diagnosed CRSwNP. Reduced miR-145-5p correlated with disease severity and was an independent ECRSwNP risk factor. In LPS-stimulated hNEpCs, miR-145-5p overexpression suppressed IL-6, IL-1β, and TNF-α. CD40 expression inversely correlated with miR-145-5p and amplified inflammatory responses mitigated by miR-145-5p overexpression. MiR-145-5p, serving as a diagnostic biomarker for CRSwNP, was negatively correlated with disease severity. MiR-145-5p overexpression attenuated inflammation in hNEpCs by targeting CD40, thereby involving itself in the progression of CRSwNP, suggesting therapeutic potential for CRSwNP management.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling lncRNA H19的下调通过ATR/CHK1信号失活增强结直肠癌的放射敏感性

IF 2.2 4区医学

Apmis Pub Date : 2025-07-16 DOI: 10.1111/apm.70052

Yuting Liang, Wanlu Zhang, Ao Dungerile, Juping Wang, Runmei Wang

{"title":"Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling","authors":"Yuting Liang, Wanlu Zhang, Ao Dungerile, Juping Wang, Runmei Wang","doi":"10.1111/apm.70052","DOIUrl":"https://doi.org/10.1111/apm.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>Radiotherapy (RT) is commonly used in the treatment of colorectal cancer (CRC), but the resistance of cancer cells to radiation remains a significant challenge in clinical practice. This study aimed to investigate whether long non-coding RNA (lncRNA) H19 could affect the sensitivity of CRC cells to radiation. The levels of H19 in CRC tissues and adjacent normal tissues, as well as in radioresistant and radiosensitive CRC tissues, were evaluated using RT-qPCR. The levels of H19 were found to be higher in CRC tissues compared to adjacent normal tissues, as well as in radioresistant CRC tissues compared to radiosensitive tissues. Depletion of H19 intensified the anti-tumor effects of radiation in CRC cells in vivo through further decreasing proliferation and enhancing apoptosis. In an HCT116 subcutaneous xenograft model, downregulation of H19 enhanced the anti-tumor effect of radiation in CRC in vivo. Mechanistically, H19 was observed to interact with ATR. Meanwhile, H19 deficiency resulted in the suppression of ATR and CHK1 phosphorylation in CRC cells, suggesting that H19 knockdown could potentially impair DNA damage repair through ATR/CHK1 signaling. Collectively, downregulation of H19 could enhance the sensitivity of CRC cells to RT by impeding DNA damage repair through the inactivation of the ATR/CHK1 signaling pathway.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Nanomaterial Strategies for Antibacterial and Antibiofilm Applications: Innovations in Bacterial Detection, Mechanistic Understanding, and Therapeutic Interventions to Address Antibiotic Resistance 抗菌和抗生素膜应用的新兴纳米材料策略：细菌检测，机制理解和治疗干预的创新，以解决抗生素耐药性

IF 2.2 4区医学

Apmis Pub Date : 2025-07-16 DOI: 10.1111/apm.70050

Manoj Kumar Goshisht

{"title":"Emerging Nanomaterial Strategies for Antibacterial and Antibiofilm Applications: Innovations in Bacterial Detection, Mechanistic Understanding, and Therapeutic Interventions to Address Antibiotic Resistance","authors":"Manoj Kumar Goshisht","doi":"10.1111/apm.70050","DOIUrl":"https://doi.org/10.1111/apm.70050","url":null,"abstract":"<div>\u0000 \u0000 <p>The rampant increase in antimicrobial resistance and stagnant pipeline of antibiotic development is a global concern. Biofilms further make the infections more persistent and incurable by providing a protective environment to the microbes. It demands the development of innovative therapeutics without delay. Nanomaterial-based therapeutics is a promising approach to combat deadly bacterial infections. The distinct shape, size, and physical characteristics of nanomaterials (NMs) make them able to target biofilms through various mechanisms and thrash recalcitrant deadly infections. This comprehensive review describes (i) historical perspective of antibiotics and their role in combating microbes; (ii) different mechanisms through which bacteria develop resistance against antibiotics; (iii) different sensing mechanisms to detect bacteria; (iv) NMs to treat planktonic bacteria; (v) NMs to treat biofilms (both oral and wound biofilms); (vi) different types of NMs (e.g., metal NMs, polymeric NMs, carbon NMs) along with photothermal effects to combat microbes.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"133 7","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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