IF 2.2 4区医学

Apmis Pub Date : 2026-08-25 DOI: 10.1111/apm.70249

Amalie Høgh Eichler, Ida Friberg Hitz, Kira Céline Koonce, Leah M. Smith, Jesper Juel Mauritzen, Nina Molin Høyland-Kroghsbo

{"title":"PMI TechNote: Bacteriophages—From the Petri Dish to Conquering the Nooks and Crannies of Difficult-to-Treat Infections","authors":"Amalie Høgh Eichler, Ida Friberg Hitz, Kira Céline Koonce, Leah M. Smith, Jesper Juel Mauritzen, Nina Molin Høyland-Kroghsbo","doi":"10.1111/apm.70249","DOIUrl":"10.1111/apm.70249","url":null,"abstract":"<p>Bacterial infections present a challenge in clinical settings due, in part, to their remarkable diversity in physiological context and treatment resistance. Bacteriophage viruses (phages) offer renewed therapeutic promise, yet their performance in vivo often differs from expectations based on standard laboratory assays. Biofilms, with their dense extracellular matrix and spatial heterogeneity, restrict phage access, diffusion, and replication. Anaerobic niches and metabolically dormant bacterial subpopulations at the biofilm core pose additional hurdles, often rendering bacteria tolerant to both antibiotics and phages. These physiological constraints are rarely captured during phage isolation, where agar-based plaque assays, oxygen-rich conditions, and rapidly growing hosts inadvertently deselect phages adapted to biofilms, anaerobic zones, or dormant states. Understanding how phage–bacterial interactions vary across these niches and how antibiotics synergize with or antagonize phage activity is essential for designing therapies with improved effectiveness beyond the Petri dish. This TechNote synthesizes key challenges for phage infection dynamics in biofilms, anaerobic environments, and dormant populations, highlighting the need for isolation and screening strategies that better reflect the physiology of clinical infections.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibrosis in One-Year Protocol Biopsies After Liver Transplantation Is Associated With Graft Loss: A Multicenter Cohort Study 肝移植术后一年方案活检纤维化与移植物损失相关：一项多中心队列研究

IF 2.2 4区医学

Apmis Pub Date : 2026-08-24 DOI: 10.1111/apm.70250

Delal Akdag, Andreas Arendtsen Rostved, Allan Rasmussen, Gro Linno Willemoe, Deepthi Chiranth, Bo-Göran Ericzon, Carl Jorns, William Bennet, Arno Nordin, Fredrik Åberg, Jens Georg Hillingsø, Susanne Dam Nielsen, Hans-Christian Pommergaard

{"title":"Fibrosis in One-Year Protocol Biopsies After Liver Transplantation Is Associated With Graft Loss: A Multicenter Cohort Study","authors":"Delal Akdag, Andreas Arendtsen Rostved, Allan Rasmussen, Gro Linno Willemoe, Deepthi Chiranth, Bo-Göran Ericzon, Carl Jorns, William Bennet, Arno Nordin, Fredrik Åberg, Jens Georg Hillingsø, Susanne Dam Nielsen, Hans-Christian Pommergaard","doi":"10.1111/apm.70250","DOIUrl":"10.1111/apm.70250","url":null,"abstract":"<p>Subclinical inflammation and fibrosis are common after liver transplantation and are associated with progressive fibrosis and cirrhosis, which are causes of graft loss. However, the association between early inflammation and fibrosis with graft loss remains insufficiently studied. In this multicenter cohort study, protocol biopsies collected one year posttransplant were assessed for inflammation and fibrosis. Fibrosis risk was assessed in recipients with paired biopsies at 1 year and ≥ 2 years posttransplantation. Associations of inflammation and fibrosis with graft loss and mortality were assessed using Cox regression. Graft loss was defined as re-transplantation or death due to liver complications. We included 446 liver transplant recipients. One year after transplantation, 54 recipients (18%) had inflammation, and 48 (12%) had fibrosis. In multivariable analysis, fibrosis was associated with graft loss, aHR 6.72 (95% CI: 1.36–33.29). In paired biopsies, recipients with fibrosis one year after transplantation had a fivefold higher risk of fibrosis at follow-up than those without. Inflammation was not associated with graft loss or mortality, and fibrosis was not associated with mortality. To conclude, recipients with fibrosis one year after transplantation had an increased risk of graft loss. Protocol biopsies remain valuable for detecting subclinical changes, pending further research to determine the effect on graft loss prevention.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serratia Bacteraemia in the North Denmark Region: A Population-Based Study (2010–2024) 北丹麦地区的沙雷菌血症：一项基于人群的研究（2010-2024）。

IF 2.2 4区医学

Apmis Pub Date : 2026-08-24 DOI: 10.1111/apm.70252

Ida M. Thiele, Kirstine K. Søgaard, Jacob Bodilsen, Hans Linde Nielsen

{"title":"Serratia Bacteraemia in the North Denmark Region: A Population-Based Study (2010–2024)","authors":"Ida M. Thiele, Kirstine K. Søgaard, Jacob Bodilsen, Hans Linde Nielsen","doi":"10.1111/apm.70252","DOIUrl":"10.1111/apm.70252","url":null,"abstract":"<p>Population-based data on <i>Serratia</i> bacteraemia are limited. We conducted a population-based cohort study of patients with a first-time episode of <i>Serratia</i> bacteraemia in the North Denmark Region between 1 January 2010 and 30 June 2024 (catchment population ~590,000). Clinical and microbiological data were obtained from medical records and the regional microbiological database. A total of 188 patients were identified. The median age was 70 years (IQR 60–78), 66% were male and 48% had a high Charlson Comorbidity Index score (≥ 3). The annual incidence remained stable at 2.1 per 100,000 person-years (95% CI 1.8–2.5). Bacteraemia was community-acquired in 38% of patients, healthcare-associated in 2% and hospital-acquired in 37%. The most common foci of infection were the urinary tract (25%), hepatobiliary tract (20%) and respiratory tract (14%), while a focus was not identified in 28% of patients. Thirty-day all-cause mortality was 24%. In an exploratory adjusted Poisson regression analysis, increasing age (RR 1.02, 95% CI 1.00–1.05) and high comorbidity (RR 2.58, 95% CI 1.01–6.63) were associated with increased mortality, whereas acquisition was not. <i>Serratia</i> bacteraemia predominantly affected older patients with substantial comorbidity and was associated with considerable mortality. Host-related factors appeared to be important determinants of outcome.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LINC00152/miR-145-5p/KLF4 ceRNA Axis: Diagnostic and Prognostic Value in Aortic Dissection LINC00152/miR-145-5p/KLF4 ceRNA轴：主动脉夹层的诊断和预后价值。

IF 2.2 4区医学

Apmis Pub Date : 2026-08-23 DOI: 10.1111/apm.70248

Xiaomei Guan, Jun Li

{"title":"LINC00152/miR-145-5p/KLF4 ceRNA Axis: Diagnostic and Prognostic Value in Aortic Dissection","authors":"Xiaomei Guan, Jun Li","doi":"10.1111/apm.70248","DOIUrl":"10.1111/apm.70248","url":null,"abstract":"<div>\u0000 \u0000 <p>Aortic dissection (AD) is a fatal cardiovascular emergency. This study explored the clinical value and mechanism of the LINC00152/miR-145-5p/KLF4 ceRNA axis in AD. 105 <span>ad</span> patients and 55 healthy controls were enrolled. LINC00152, miR-145-5p, and KLF4 levels were measured by qRT-PCR. ROC and Cox regression analyses assessed diagnostic and prognostic value. Dual-luciferase reporter and rescue experiments in Ang II-induced HASMCs validated the ceRNA axis. As a result, LINC00152 and KLF4 were upregulated, while miR-145-5p was downregulated in AD patients. The three molecules showed good diagnostic efficacy (combined AUC = 0.9087). LINC00152, KLF4, and their joint risk signature were independent prognostic factors for adverse outcomes. Mechanistically, LINC00152 sponged miR-145-5p to upregulate KLF4, promoting pro-inflammatory factors (TNF-α, IL-1β) and matrix metalloproteinases (MMP-2, MMP-9) in HASMCs. In conclusion, the LINC00152/miR-145-5p/KLF4 ceRNA axis shows preliminary diagnostic and prognostic potential for AD, and drives AD development by regulating vascular inflammation and extracellular matrix degradation, which may serve as a candidate molecular target for subsequent translational research.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of HPV Vaccination on Juvenile Laryngeal Papillomas and Conjunctival Papillomas in Denmark: Incidence and Analysis of HPV Subtypes in Relation to Vaccination HPV疫苗接种对丹麦青少年喉部乳头状瘤和结膜乳头状瘤的影响：HPV亚型与疫苗接种相关的发病率和分析

IF 2.2 4区医学

Apmis Pub Date : 2026-08-19 DOI: 10.1111/apm.70240

Mathilde Aakilde, Joachim Hansen, Ida-Marie Jacobsen, Simone Kloch Bendtsen, Giedrius Lelkaitis, Steffen Heegaard, Christian von Buchwald

{"title":"Impact of HPV Vaccination on Juvenile Laryngeal Papillomas and Conjunctival Papillomas in Denmark: Incidence and Analysis of HPV Subtypes in Relation to Vaccination","authors":"Mathilde Aakilde, Joachim Hansen, Ida-Marie Jacobsen, Simone Kloch Bendtsen, Giedrius Lelkaitis, Steffen Heegaard, Christian von Buchwald","doi":"10.1111/apm.70240","DOIUrl":"https://doi.org/10.1111/apm.70240","url":null,"abstract":"<p>Papillomas, predominantly caused by low-risk Human papillomavirus (HPV), can affect the respiratory tract and conjunctiva. This nationwide Danish registry study assesses the HPV vaccination programme's effectiveness in preventing juvenile laryngeal and conjunctival papillomas. Incidence rates were calculated using national registry data from 2005/2008 to 2024. In the Danish Pathology Registry, 33 juvenile laryngeal and 134 conjunctival papilloma patients were identified using SNOMED codes. HPV subtyping was performed in-house for laryngeal biopsies lacking prior data, yielding results for 27 patients. HPV-6 or -11 was identified in 74.1% of cases. Vaccination status was retrieved for conjunctival patients and for mothers of laryngeal patients. The incidence rate of juvenile laryngeal papillomas was 0.190 per 100,000 (2005–2013) and 0.107 (2014–2024) (<i>p</i> = 0.145). No mothers of laryngeal patients had received HPV vaccination before delivery. Conjunctival papilloma incidence declined from 2008 to 2024, with an annual percent change of −5.3% (<i>p</i> = 0.002). A total of 22 conjunctival patients were vaccinated before first biopsy, with a median interval of 5 years and 8 months between first dose and biopsy. In Denmark, juvenile laryngeal papilloma incidence remained low and occurred only in children of unvaccinated mothers, while conjunctival papillomas showed a significant decline consistent with a protective effect of the HPV vaccination programme.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70240","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic and Prognostic Value of miR-27a-5p in Childhood Severe Pneumonia Through Targeted Regulation of FOXO1 miR-27a-5p通过靶向调控fox01在儿童重症肺炎中的诊断及预后价值

IF 2.2 4区医学

Apmis Pub Date : 2026-08-11 DOI: 10.1111/apm.70241

Yupei Liu, Haobin Wang, Jianzhu He, Ludan Deng, Xiao Zhou

{"title":"Diagnostic and Prognostic Value of miR-27a-5p in Childhood Severe Pneumonia Through Targeted Regulation of FOXO1","authors":"Yupei Liu, Haobin Wang, Jianzhu He, Ludan Deng, Xiao Zhou","doi":"10.1111/apm.70241","DOIUrl":"10.1111/apm.70241","url":null,"abstract":"<div>\u0000 \u0000 <p>Severe pneumonia in children deteriorates rapidly and is very destructive. miR-27a-5p has been shown to function in multiple cancers and inflammatory conditions, but its role in severe pneumonia is unknown. To investigate the diagnostic and prognostic value of miR-27a-5p for severe pneumonia in children. This study included 146 children with severe pneumonia as subjects and 123 children with mild pneumonia as controls. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was employed to assay miR-27a-5p and FOXO1 levels. ROC and Kaplan–Meier curves were employed to assess the diagnostic and prognostic values. Cell viability and apoptosis were determined by cell counting kit-8 (CCK-8) and flow cytometry. The concentrations of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β were measured using enzyme-linked immunosorbent assay. Luciferase reporter assay for miR-27a-5p targeting FOXO1. miR-27a-5p level was higher in severe pneumonia. miR-27a-5p has a good diagnostic and prognostic value for severe pneumonia. miR-27a-5p down-regulation promotes cell viability and inhibits apoptosis and inflammatory factor production. FOXO1 is a target of miR-27a-5p. And FOXO1 may reverse the effects of miR-27a-5p on the biological function of HFL1 cell. In conclusion, miR-27a-5p may be a potential pneumonia marker by negatively regulating FOXO1 to worsen pneumonia.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Sub-Classification Suggests IBD Unclassified as a Distinct Subtype of Inflammatory Bowel Disease: Insights From a 3-Gene Mucosal Signature 分子亚分类表明IBD未分类为炎症性肠病的独特亚型：来自3基因粘膜特征的见解

IF 2.2 4区医学

Apmis Pub Date : 2026-08-10 DOI: 10.1111/apm.70245

Jaslin P. James, Ib Jarle Christensen, Boye S. Nielsen, Ebbe Langholz, Estrid Høgdall, Lene Buhl Riis

{"title":"Molecular Sub-Classification Suggests IBD Unclassified as a Distinct Subtype of Inflammatory Bowel Disease: Insights From a 3-Gene Mucosal Signature","authors":"Jaslin P. James, Ib Jarle Christensen, Boye S. Nielsen, Ebbe Langholz, Estrid Høgdall, Lene Buhl Riis","doi":"10.1111/apm.70245","DOIUrl":"10.1111/apm.70245","url":null,"abstract":"<p>Differentiating Crohn's disease (CD) from ulcerative colitis (UC) is challenging, often resulting as Inflammatory bowel disease unclassified (IBDU). Based on targeted RNA sequencing, we recently reported a 3-gene signature algorithm that distinguished CD from UC. This study evaluated its ability to classify IBDU cases and explore disease biology. We analyzed 197 mucosal FFPE biopsies (inflamed and non-inflamed) from 113 individuals at diagnosis: CD (<i>n</i> = 42), UC (<i>n</i> = 41) and IBDU (<i>n</i> = 102; follow-up: 0–23 years (IQR)). Expression levels of ANXA1, PI3, and VDR mRNAs were measured by RT-qPCR, and assessed using logistic regression, and ROC curve analysis. The algorithm discriminated CD from UC with AUC values > 0.70 in 6 out of 8 patient subsets, peaking in inflamed adult biopsies (0.78, 95% CI: 0.64–0.92). At 80% sensitivity, specificity ranged from 46% to 58%, replicating previous cohort findings and confirming reproducibility. Application of the algorithm to the IBDU cases showed low classification accuracy, with frequent misclassification of cases that were later received definitive diagnoses. Incorporation of additional IBDU-specific features did not improve performance. While the 3-gene signature algorithm demonstrates moderate ability to distinguish CD from UC, its limited performance in IBDU cases highlights fundamental constraints of binary classification approaches in IBD.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.70245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced Circulating Angiopoietin-Like Protein Levels in Crimean-Congo Hemorrhagic Fever 克里米亚-刚果出血热降低循环血管生成素样蛋白水平。

IF 2.2 4区医学

Apmis Pub Date : 2026-08-10 DOI: 10.1111/apm.70246

Sümeyye Yeşildağ, Seyit Ali Büyüktuna, Caner Öksüz, Halef Okan Doğan

{"title":"Reduced Circulating Angiopoietin-Like Protein Levels in Crimean-Congo Hemorrhagic Fever","authors":"Sümeyye Yeşildağ, Seyit Ali Büyüktuna, Caner Öksüz, Halef Okan Doğan","doi":"10.1111/apm.70246","DOIUrl":"10.1111/apm.70246","url":null,"abstract":"<div>\u0000 \u0000 <p>Crimean-Congo Hemorrhagic Fever (CCHF) is a life-threatening zoonotic viral disease characterized by endothelial dysfunction, coagulopathy, and systemic inflammation. Angiopoietin-like proteins (ANGPTLs) regulate vascular integrity, lipid metabolism, and inflammatory responses; however, their roles in the pathogenesis of CCHF remain unclear. In this prospective case–control study, serum levels of ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL6, and ANGPTL8 were measured by enzyme-linked immunosorbent assay (ELISA) in 60 patients with laboratory-confirmed CCHF and 30 healthy controls. Standard laboratory parameters were recorded, and correlations between ANGPTLs and inflammatory, coagulation, and metabolic markers were analyzed. Receiver operating characteristic (ROC) analysis was performed to assess diagnostic performance and exploratory mortality-discrimination ability. All measured ANGPTL levels were significantly lower in CCHF patients than in healthy controls. However, no statistically significant differences in ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL6, or ANGPTL8 levels were observed between survivors and non-survivors. Although ANGPTL2 and ANGPTL3 yielded statistically significant area under the curve (AUC) values for mortality, the small number of fatal cases limits the strength of this finding. Overall, reduced ANGPTL levels were associated with disease-related inflammatory, endothelial, coagulation-related, and metabolic alterations, but these findings do not support their role as prognostic markers for mortality or establish a causal role in CCHF pathogenesis.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Microbiologists as Decision-Support Specialists: Clinicians' Views on Microbiology Consultation, Stewardship, and the Future Role of the Specialty 作为决策支持专家的临床微生物学家：临床医生对微生物学咨询、管理和该专业未来角色的看法。

IF 2.2 4区医学

Apmis Pub Date : 2026-07-29 DOI: 10.1111/apm.70243

Einar Nilsen

{"title":"Clinical Microbiologists as Decision-Support Specialists: Clinicians' Views on Microbiology Consultation, Stewardship, and the Future Role of the Specialty","authors":"Einar Nilsen","doi":"10.1111/apm.70243","DOIUrl":"10.1111/apm.70243","url":null,"abstract":"<div>\u0000 \u0000 <p>The clinical value of microbiological diagnostics depends on whether results are translated into bedside decisions. There are differing opinions and traditions around what role the physician microbiologist should have in this translation from laboratory results to treatment. We performed a cross-sectional questionnaire study among hospital clinicians in Møre and Romsdal Health Trust, Norway, to explore perceptions of microbiology-based decision support and the future role of physician clinical microbiologists. A total of 251 clinicians responded. Of these, 85.7% treated patients with suspected or confirmed infection more often than monthly. Among this key clinical subgroup, 90.7% considered microbiology decision support to be in the top two of five categories of usefulness; only 0.8% found it not useful. 94.9% found the advice professionally relevant, 95.8% found it easy to translate into action, 98.1% reported usually following recommendations, 88.8% reported that the support contributed to better antibiotic use, 87.0% to faster or safer clinical decisions, and 97.7% were positive toward an active clinical advisory role by physician clinical microbiologists. The findings support a clinically integrated model where clinical microbiologists contribute not only as laboratory specialists, but as decision-support partners in infection management.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UBC9 and PI3K Expression in Colorectal Cancer: Possible Prognostic Role UBC9和PI3K在结直肠癌中的表达：可能的预后作用

IF 2.2 4区医学

Apmis Pub Date : 2026-07-29 DOI: 10.1111/apm.70242

Ola Nasser, Ahmed Rabea, Reham Diaa

{"title":"UBC9 and PI3K Expression in Colorectal Cancer: Possible Prognostic Role","authors":"Ola Nasser, Ahmed Rabea, Reham Diaa","doi":"10.1111/apm.70242","DOIUrl":"10.1111/apm.70242","url":null,"abstract":"<div>\u0000 \u0000 <p>Colorectal cancer (CRC) is the second cause of cancer-related deaths worldwide. Although ubiquitin-conjugating enzyme 9 (UBC9) expression has been implicated in a variety of malignancies, its precise role in CRC remains poorly understood. Also Phosphatidylinositol 3-kinase (PI3K) signaling is known to enhance cellular proliferation in various cancers. This study aims to investigate the expression of UBC9 and PI3K in CRC, prognostic and predictive significance, and association with patients' survival. A total of 140 archival samples of CRC, 70 normal colonic tissue, and 40 metastatic lymph nodes were included. Immunohistochemistry was done for UBC9 and PI3K antibodies, and appropriate statistical analysis of results was performed. UBC9 and PI3K are highly expressed in tumor cells, where co-high expression is significantly associated with high grade, advanced stage, high lymph node ratio (LNR), lympho-vascular invasion (LVI), low tumor infiltration lymphocytes (TILs), chemo-resistance, and positive KRAS mutation, with the poorest median survival (17 months) in patients. Significant positive association was revealed between both molecules in metastatic lymph nodes. UBC9 and PI3K are related to CRC poor survival and resistance to treatment. A potential combined role of both molecules in colorectal carcinogenesis could be found.</p>\u0000 </div>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"134 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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