Applied Soil Ecology最新文献

{"title":"Exogenous selenium addition alters key community composition and decreases the abundance of diazotrophs in soybean rhizosphere","authors":"Jianrong Zhao ,&nbsp;Shiyu Hu ,&nbsp;Lei Shan ,&nbsp;Youpeng Wen ,&nbsp;Xuebin Yin ,&nbsp;Ruilin Huang","doi":"10.1016/j.apsoil.2025.106468","DOIUrl":"10.1016/j.apsoil.2025.106468","url":null,"abstract":"<div><div>Exogenous selenium addition is an effective strategy to enhance selenium content in legume plants. However, its impact on the composition and nitrogen-fixing potential of rhizosphere diazotrophic communities remains poorly understood. To elucidate the effects of exogenous selenium on rhizosphere nitrogen-fixing potential and the underlying mechanisms, we conducted pot experiments with treatments including selenium addition (Se), chemical fertilization (CF), and their combination (CFSe). Our results showed that CF and CFSe significantly reduced diazotrophic community richness (~39.36 % and 40.69 %, respectively) and diversity (~10.55 % and 20.29 %, respectively), whereas Se had no significant effect. Furthermore, Se, CF, and CFSe treatments significantly reduced <em>nifH</em> gene copy numbers by 54.18 %, 83.96 %, and 91.06 %, respectively. Random Forest and redundancy analyses identified total nitrogen (TN), available nitrogen (AN), total phosphorus (TP), and available selenium as critical abiotic factors influencing the α- and β-diversity of diazotrophs. Co-occurrence network analysis revealed that diazotrophs within module 1 were particularly sensitive to selenium addition and fertilization, with their community stability being crucial for maintaining diazotrophic α diversity. Notably, Se and CF treatments appeared to suppress rhizobia-legume symbiosis by increasing the abundance of non-symbiotic <em>Cyanobacteria</em> in module 1, thereby reducing nodule density in legumes. These findings indicate that exogenous selenium addition can profoundly alter the composition and function of diazotrophic communities, reducing their nitrogen-fixing potential and modulating plant-microbe interactions.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106468"},"PeriodicalIF":5.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar and iron minerals facilitate the reduction of pollution and sequestration of carbon in chloramphenicol-contaminated soil under dry conditions","authors":"Pengfei Cheng ,&nbsp;Junliang Chen ,&nbsp;Ran Xu ,&nbsp;Qin Tian ,&nbsp;Xiaobo Luo ,&nbsp;Zhicong Dai ,&nbsp;Zhiliang Li ,&nbsp;Yao Lu ,&nbsp;Lianghui Li ,&nbsp;Kuan Cheng ,&nbsp;Chao Guo ,&nbsp;Guanlin Li ,&nbsp;Daolin Du ,&nbsp;Tongxu Liu","doi":"10.1016/j.apsoil.2025.106458","DOIUrl":"10.1016/j.apsoil.2025.106458","url":null,"abstract":"<div><div>Addressing chloramphenicol (CAP) contamination and enhancing soil organic carbon (SOC) sequestration are vital for environmental sustainability. This research explored the combined influence of biochar (BC), iron mineral (Fe₂O₃), and their composite (FeBC) on CAP degradation and SOC distribution across various moisture regimes, including dry, wet, and dry-wet alternation (DWA) conditions. DWA conditions were the most effective at reducing bioavailable CAP residues. However, FeBC exhibited superior CAP removal efficiency over individual BC or Fe₂O₃ treatments, with the greatest reduction observed under dry conditions. Compared with those of the control without additives, reduction rates reached 46.98 % (DRY), 14.95 % (WET), and 23.81 % (DWA), respectively. BC primarily increased particulate organic carbon (POC) content, with a notable increase of 23 % under dry conditions. In contrast, Fe₂O₃ markedly increased mineral-associated organic carbon (MAOC) content by 62 %, with the strongest effect observed under dry conditions. However, no synergistic interaction between BC and Fe₂O₃ was detected in promoting carbon sequestration. Redundancy analysis indicated a negative correlation between soil moisture and both POC and MAOC, whereas BC and Fe₂O₃ showed positive correlations with POC and MAOC, respectively. Additionally, soil moisture, BC, and Fe₂O₃ influenced soil physicochemical properties and enzyme activities, which were closely associated with CAP removal and carbon sequestration processes. The transformation of bioavailable CAP was correlated with SOC forms: enhanced POC by BC and enhanced MAOC by Fe₂O₃ facilitated the fixation of CAP. This study provides theoretical support for the development of integrated “pollution control and carbon sequestration” technologies in soils.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106458"},"PeriodicalIF":5.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wind farm-induced redistribution of soil organic matter: Molecular insights into dissolved organic matter and microbial interactions","authors":"Qingyi Li ,&nbsp;Jun Chen ,&nbsp;Siwei Peng ,&nbsp;Guozhu Zhang ,&nbsp;Zexu Chen ,&nbsp;Boyuan Yang ,&nbsp;Yu Zhang ,&nbsp;Siying Cai ,&nbsp;Weijun Zhang","doi":"10.1016/j.apsoil.2025.106459","DOIUrl":"10.1016/j.apsoil.2025.106459","url":null,"abstract":"<div><div>Wind energy has become a cornerstone of renewable energy development, yet its environmental impacts on soil systems remain insufficiently understood. In particular, how long-term wind farm operation influences organic matter dynamics at the molecular level has received limited attention. In this study, we first surveyed three onshore wind farms across distinct climatic zones in China and found that long-term wind farm operation significantly enriched soil organic carbon, total nitrogen, nitrate‑nitrogen, and ammonium‑nitrogen within operational zones compared to undisturbed reference areas. Based on this, we focused on one inland wind farm with the highest wind energy potential and the most complete wind regime records as a case study site, to explore the molecular composition and transformation of dissolved organic matter (DOM) in greater depth. By integrating ultrahigh-resolution Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), spectroscopic analyses, and high-throughput sequencing, we reveal that wind-induced microclimatic shifts may contribute to a significant fertile island effect, characterized by increased nutrient availability and enhanced carbon accumulation in wind farm soils. Wind-driven physical transport further shapes the spatial differentiation of DOM: upwind areas are characterized by highly oxidized and humified DOM components, whereas downwind areas accumulate fresh, labile organic compounds. This gradient also drives a transition in microbial trophic strategies, from oligotrophic taxa dominating upwind soils to copiotrophic taxa thriving downwind, with their distinct substrate preferences reinforcing DOM spatial heterogeneity. These findings offer molecular-level insights into wind farm-induced alterations in soil carbon cycling and underscore the need to incorporate biogeochemical perspectives into environmental assessments of renewable energy infrastructure.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106459"},"PeriodicalIF":5.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial network stability in chlorpyrifos-contaminated soils is improved by microbial inoculant application","authors":"Chunxiao Wu ,&nbsp;Zefang Tong ,&nbsp;Meihuan Lu ,&nbsp;Lijun Li ,&nbsp;Yalei Pan ,&nbsp;Yinghui Ma","doi":"10.1016/j.apsoil.2025.106454","DOIUrl":"10.1016/j.apsoil.2025.106454","url":null,"abstract":"<div><div>Soil degradation caused by organophosphorus pesticide (OPP) residues poses a critical environmental challenge. However, the remediation efficiency of microbial inoculants, as well as the underlying mechanisms, in OPP-contaminated soils remain unclear. In this study, microcosm-controlled experiments combined with high-throughput sequencing were performed to systematically investigate the remediation efficiency of microbial inoculants, as well as the underlying mechanisms, in OPP-contaminated soils. Compared with the chlorpyrifos-exclusively treatment (CPF), the chlorpyrifos plus microbial inoculant treatment (MI) significantly increased the soil organic carbon (SOC), total nitrogen (TN), and available phosphorus (AP) contents (<em>p</em> &lt; 0.05) while markedly increasing the β-glucosidase (BG) and catalase (CAT) activities by up to 202.8 % and 30.9 %, respectively. Notably, with prolonged incubation, the AP and available potassium (AK) contents decreased in CPF treatment, whereas they increased under the MI treatment. The MI treatment restructured the microbial community composition by enriching copiotrophic microorganisms (Proteobacteria) while suppressing oligotrophic microorganisms (Acidobacteria). Network analysis revealed that the MI treatment improved the modularity and robustness of soil microbial networks. Mechanistically, MI treatment enhanced the stability of microbial communities to environmental disturbances by mediating nutrient-regulated soil enzyme activities. Random forest modeling identified SOC and BG as pivotal regulators of microbial network stability. This study confirms the theory of synergistic adaptation between microbial network complexity and stability, providing a scientific basis to optimize soil bioremediation technologies for sustainable agriculture.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106454"},"PeriodicalIF":5.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acidified slurry reduces microbial and soil organic matter priming compared to conventional slurry","authors":"Yusra Zireeni ,&nbsp;Robert W. Brown ,&nbsp;Davey L. Jones ,&nbsp;David R. Chadwick","doi":"10.1016/j.apsoil.2025.106457","DOIUrl":"10.1016/j.apsoil.2025.106457","url":null,"abstract":"<div><div>Intensive agricultural practices typically lead to a loss of soil organic carbon (SOC). Consequently, there is a need to develop more sustainable management strategies that promote greater carbon (C) storage in soil. Slurry application is a management option often used to maintain soil organic matter (SOM) stocks and recycle and replenish nutrients essential for crop growth. Slurry acidification with sulfuric acid (H₂SO₄) is a method known to enhance slurry nitrogen (N) retention (through reduced ammonia emissions), however, its broader impacts on SOC turnover are less well understood. This study assessed the priming effect of applications of conventional cattle slurry (pH 6.90 ± 0.06, <em>n</em> = 3) and acidified cattle slurry (pH 5.5) on three distinct soil C pools; ¹⁴C-labelled microbial biomass (apparent priming), fresh particulate organic matter (POC; ¹⁴C-labelled fresh grass crop residues; GCR) and ¹⁴C-labelled quasi-stable SOM (real priming) in a series of laboratory assays, over 6 months. All slurry treatments resulted in positive priming compared to the control (no treatment). The results indicated that slurry acidification reduced the real and apparent priming effects, relative to conventional slurry application, by 7.9 % and 11.3 %, respectively. Acidified slurry application also increased mineralisation of fresh GCR inputs promoting decomposition and nutrient release. In all pools, the effect of slurry addition was short-lived, lasting no more than six months. This suggests that applying acidified slurry may reduce C losses, potentially enhancing soil quality compared to conventional slurry, while also offering the added benefits of reduced N losses.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106457"},"PeriodicalIF":5.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent responses of N2O emissions to biodegradable microplastics and warming in contrasting subtropical soils of Southwest China","authors":"Fuli Li ,&nbsp;Xin Zhang ,&nbsp;Di Wu ,&nbsp;Zhu Chen ,&nbsp;Yuechen Tan ,&nbsp;Yifei Wang ,&nbsp;Wanping Zhang ,&nbsp;Roland Bol","doi":"10.1016/j.apsoil.2025.106452","DOIUrl":"10.1016/j.apsoil.2025.106452","url":null,"abstract":"<div><div>Microplastic pollution and climate change are increasingly recognized as key drivers of biogeochemical processes in soil ecosystems, however, their interactive effects on greenhouse gas (GHG) emissions remain poorly understood. Here, we conducted a 168-h laboratory incubation. We used two contrasting subtropical soils from the karst region of Southwest China: a natural Masson pine (<em>Pinus massoniana</em>) forest soil and an adjacent farmland soil converted from the same forest and continuously cultivated with chili (<em>Capsicum annuum</em>) for two decades. Treatments included biodegradable polylactic acid (PLA) microplastics at 0 %, 1 %, and 10 % (<em>w</em>/w) and two temperatures (25 and 30 °C). Incubations were run under oxic (20 % O₂) and anoxic (0 % O₂) conditions. Soil analyses and quantitative PCR conducted prior to incubation revealed that farmland soils had higher NO₃⁻ (+229 %) and NH₄⁺ (+45 %) concentration, enzymatic activity (β-1,4-<em>N</em>-acetyl-glucosaminidase: +16 %, β-1,4-glucosidase: +15 %), microbial Simpson diversity (+8 %), and lower DOC concentration (−64 %) compared to forest soils. Additionally, farmland soil had significantly higher abundances of denitrification-related functional genes (+2 %–7 %) and lower ammonia-oxidizing bacteria (<em>amoA-AOB</em>, −2 %). These changes potential strongly promoted cumulative CO₂ (+18 %) and N₂O (+384 %) emissions in farmland soils (CO₂: 133 ± 16 g C kg⁻¹, N₂O: 94 ± 19 mg N kg⁻¹) compared to natural forest. CO₂ emissions showed additive effects, with maximum CO₂ emissions under 10 % <em>w</em>/w PLA at 30 °C. Interestingly, a nonlinear response in N₂O emission was observed: emissions peaked at 1 % PLA (53 ± 11 mg N kg⁻¹) but declined at 10 % (16 ± 4 mg N kg⁻¹), suggesting a concentration threshold potentially due to microbial priming at low PLA and substrate limitation at higher doses. Our results state that forest-to-farmland conversion substantially increases CO₂ and N₂O emissions due to elevated inorganic nitrogen and denitrifier gene abundance. Moreover, we reveal a threshold effect of microplastics on N₂O fluxes and a synergistic effect of warming and microplastics on CO₂, but not N₂O, emission. These results highlight the need to consider land use history, pollutant concentration, and gas-specific microbial pathways when predicting soil GHG responses under future climate scenarios. However, these insights are based on short-term incubation, and further long-term studies are required to validate whether these patterns persist or shift over extended exposure.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106452"},"PeriodicalIF":5.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Winter bioaugmentation of co-contaminated soil: Differential effects in greenhouse and open-field settings on microbial communities","authors":"Zijun Ni ,&nbsp;Xiaorong Zhang ,&nbsp;Chunyun Jia ,&nbsp;Muhammad Zaffar Hashmi ,&nbsp;Shuhai Guo ,&nbsp;Huaqi Pan ,&nbsp;Zongqiang Gong","doi":"10.1016/j.apsoil.2025.106446","DOIUrl":"10.1016/j.apsoil.2025.106446","url":null,"abstract":"<div><div>This study evaluated a winter bioaugmentation strategy using the psychrotolerant strain <em>Microbacterium arborescens</em> SMB19 to remediate antibiotic–heavy metal co-contaminated soils and examined its effects on microbial community functions under two contrasting environments: greenhouse warming (WTM) and open-field non-warming (CTM). Results showed that SMB19 effectively reduced antibiotic residues and bioavailable Zn concentrations. Under CTM, remediation was more effective, with higher antibiotic degradation rates and greater reductions in bioavailable metals. However, the abundance of mobile genetic elements (MGEs) and antibiotic resistance genes (ARGs) increased. In contrast, under WTM, the synergistic effect between SMB19 and indigenous microbes was weaker, but the ARG and MGE levels significantly decreased. Mantel test results revealed a stronger correlation between ARGs and microbial communities in the WTM. Network analysis showed that microbial functional groups were more dispersed in the WTM than in the highly clustered structure observed in the CTM. Functionally, CTM-enriched microbial taxa were involved in pollutant remediation and element cycling, whereas WTM-favored taxa were related to nitrogen cycling and greenhouse-specific adaptations. Functional predictions further indicated that WTM enhanced antibiotic biosynthesis pathways and CTM promoted organic matter degradation. These findings suggest that exogenous bacteria-based bioaugmentation holds great promise for cold-region soil remediation; however, greenhouse conditions must be carefully optimized to balance pollutant removal efficiency and the risk of ARG dissemination.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106446"},"PeriodicalIF":5.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable microplastics increase fungi-mediated N2O emission by rapidly releasing dissolved organic matters","authors":"Lei Zhong ,&nbsp;Yufeng Song ,&nbsp;Xiaoxian Cai ,&nbsp;Ping Wang ,&nbsp;Guanqi Yu ,&nbsp;Jia Liu ,&nbsp;Xiaoxuan Su ,&nbsp;Xingliang Xu","doi":"10.1016/j.apsoil.2025.106444","DOIUrl":"10.1016/j.apsoil.2025.106444","url":null,"abstract":"<div><div>Plastic pollution in paddy fields disrupts soil nitrogen biogeochemistry, prompting the introduction of biodegradable plastics like PBAT (poly (butylene adipate-<em>co</em>-terephthalate)) as a potential mitigation strategy to plastic pollution reduction. However, the effects of nonbiodegradable and biodegradable microplastics on the nitrogen cycle remain unclear. To clarify this, we conducted an incubation experiment, N₂O isotopocules, and molecular analysis to assess the impact of additives from PBAT and non-biodegradable (polyethylene, PE) microplastics. This study aimed to investigate their effects on N₂O emission from bacterial, fungal, and chemical denitrification. The results showed that PE reduced N₂O production potential from denitrification (D_N2O) and bacterial denitrification (BD_N2O), while PBAT (poly (butylene adipate-<em>co</em>-terephthalate)) increased D_N2O and fungal-derived N₂O (FD_N2O) but decreased BD_N2O. PE inhibited N₂O production via bacterial denitrification due to the toxicity of plastic additives such as dibutyl phthalate (DBP) and diethylhexyl phthalate (DEHP). In contrast, PBAT enhanced N₂O production via fungal pathways by facilitating the release of dissolved organic carbon. Bacterial denitrification accounted for 43–56% of total N₂O production potential (D_N2O) in PE treatments but only 28–50% in PBAT treatments. These findings highlight the short-term risks posed by biodegradable microplastics in elevating N₂O emissions and reveal new dimensions of the influence of microplastics on greenhouse gas emissions from agricultural soils.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106444"},"PeriodicalIF":5.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of sustainable management practices on the bacterial community in different European croplands","authors":"J. Cuartero ,&nbsp;B. Frey ,&nbsp;R. Zornoza ,&nbsp;V. Sánchez-Navarro ,&nbsp;L. Canfora ,&nbsp;O. Özbolat ,&nbsp;M. Egea-Cortines ,&nbsp;R. Farina ,&nbsp;H. Fritze ,&nbsp;T. Tuomivirta ,&nbsp;K. Lång ,&nbsp;R. Lemola ,&nbsp;J. Álvaro-Fuentes ,&nbsp;E. Huerta-Lwanga ,&nbsp;J.A. Pascual ,&nbsp;M. Ros","doi":"10.1016/j.apsoil.2025.106456","DOIUrl":"10.1016/j.apsoil.2025.106456","url":null,"abstract":"<div><div>Currently, monitoring Europe's soils is crucial since over 60 % of these soils are experiencing different types of degradation, which can potentially affect food production. Recently, a shift toward sustainable soil management has occurred. This shift away from conventional management is postulated to increase soil microbial diversity. However, it is unclear whether sustainable management can shift bacterial community across Europe. The Diverfarming project uses case studies to explore how diversified cropping systems with low-input practices can increase soil fertility, sequester carbon, and increase microbial diversity under differing climate conditions. To explore this, we employed metabarcoding sequencing to amplify the 16S rRNA region and soil chemical properties to assess the effects of organic amendment, rotation/intercropping and diversification with reduced tillage compared to conventional systems in different case studies. We observed that richness and Shannon index were mainly affected by climate and soil chemical properties but not by diversification. However, diversification changed the microbial community and enhanced potential microbial functionality, especially diversification of organic amendments, which also increase total organic carbon and nitrogen. We identified specific bacterial taxa associated with diversification, such as <em>Rubrobacter</em>, <em>MND1</em>, <em>Pontibacter</em> and <em>Sphingomonas</em>, highlighting the potential benefits of some species of these genera in diversification management ecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106456"},"PeriodicalIF":5.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of straw return: Microbial agent-driven lignocellulose degradation and soil nitrogen availability in wheat fields","authors":"Hongsen Zhang ,&nbsp;Shile Peng ,&nbsp;Tongyu Dai ,&nbsp;Xin Wang ,&nbsp;Xuyi Zhang ,&nbsp;Lifang Sun ,&nbsp;Fengqin Wang ,&nbsp;Hui Xie ,&nbsp;Guotao Mao ,&nbsp;Yonghua Wang ,&nbsp;Andong Song","doi":"10.1016/j.apsoil.2025.106450","DOIUrl":"10.1016/j.apsoil.2025.106450","url":null,"abstract":"<div><div>Straw returning to field is considered as a sustainable way of straw utilization. In this study, a novel and effective microbial agent (MA) was applied to the straw returning soil during the winter wheat growing season. The addition of MA increased the weight loss ratio of corn straw from 6.94 % to 25.87 % after 10 days under laboratory conditions, especially for the degradation of cellulose and hemicellulose. In the field experiment, the addition of MA also accelerated the degradation of corn straw in soil, consequently increased the content of total nitrogen in soil. The soil metagenomics analysis showed that relative abundance of <em>Nocardioides</em>, <em>Solirubrobacter</em> and <em>Arthrobacter</em> were increased at the species level which could enhance corn straw degradation in soil. The abundances of GHs (glycoside hydrolase) and AAs (auxiliary active enzyme) family genes increased and this was likely to have promoted the degradation of corn straw. In addition, the abundances of nitrifying and denitrifying genes such as amoA-A, amoB-B, amoC-C, nxrA, narH, and narI were increased at the overwintering stage and booting stage which could improve the nitrogen cycling in soil. MA promoted the degradation of corn straw, increased the expression of nitrification and denitrification genes, However, it did not result in a statistically significant increase in wheat yield. This was an effective way to promote the sustainable development of agricultural production through functional microbial resources mining and MA application.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"215 ","pages":"Article 106450"},"PeriodicalIF":5.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}