Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology最新文献_第8页

[The combined treatment of juvenile outset recurrent respiratory papillomatosis]. 【青少年复发性呼吸道乳头状瘤病的综合治疗】。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Jin Xie, Pin Dong, Keyong Li, Jie Wang, Bin Jin, Jia Zhang

引用次数: 0

[Effects of conditioning noise exposure on the expression of F-actin]. [调节噪声暴露对F-actin表达的影响]。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Hua Cao, Mingmin Dong, Chunhong Zhao, Honghui Li

{"title":"[Effects of conditioning noise exposure on the expression of F-actin].","authors":"Hua Cao, Mingmin Dong, Chunhong Zhao, Honghui Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To obtain more information on the distribution of F-actin in the guinea pig cochlea, to observe the effects of conditioning noise exposure on the expression of F-actin and to study this \"toughening\" phenomenon mechanisms.Method: An octave band noise centered at 1 kHz at 95 dB SPL for 8 hours a day was used as conditioning noise. Animals were killed at different time points after different conditioning noise exposure. The organs of Corti were labelled by FITC-phalloidin and examined using laser scanning confocal microscopy following cochlear preparation.Result: High intensity labeling of F-actin was seen in both inner and outer hair cells, specifically in stereocilia, cuticular plates. In the circumferential rings around the apical periphery of each cell, the head plates of the pillar cells, the lowest portion of OHC bodies, which represented the Deiters cup and in the phalangeal processes of Deiters cell, the strong labelling of F-actin was also seen. F-actin was found in the out pillar cells' body. F-actin labelling of OHCs in differently conditioning exposed animals was different.Conclusion: (1) Conditioning exposure can change the expression of F-actin in guinea pig cochlea. (2) In the organ of Corti of guinea pig, F-actin distributes not only in the portions previously reported, but also in the out pillar cell' s body. (3) A reduced expression of F-actin in out hair cell after conditioning exposure may be connected to the \"toughening\" phenomenon.","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 22","pages":"1035-7"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26574628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of mACh receptor ml-m5 subunits in the vestibular endorgans of rat]. [大鼠前庭内器官马赫受体ml-m5亚基的表达]。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Huanmao Cheng, Weijia Kong, Qi Yao

引用次数: 0

[Establishment and evaluation on the animal model of vestibular compensation]. 前庭代偿动物模型的建立与评价。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Yaming Zhang, Weijia Kong, Jun Liu

引用次数: 0

[The hearing manifestations of 350 patients of AIDS]. 350例艾滋病患者的听力表现

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Ying Wang, Haibo Yang, Mingmin Dong

引用次数: 0

[Expression of telomerase reverse transcriptase and its relationship with expression of c-myc in laryngeal squamous cell carcinomas]. [端粒酶逆转录酶在喉鳞癌中的表达及其与c-myc表达的关系]。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Zhiling Chen, Zhenghu Wu, Xing Guo, Zuliang Wu, Manger Jiang

{"title":"[Expression of telomerase reverse transcriptase and its relationship with expression of c-myc in laryngeal squamous cell carcinomas].","authors":"Zhiling Chen, Zhenghu Wu, Xing Guo, Zuliang Wu, Manger Jiang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the expression of human telomerase reverse transcriptase (hTERT) and c-myc and their relationship with clinicopathological parameter in laryngeal squamous cell carcinoma (LSCC).Method: The expression of hTERT protein and c-myc protein in 39 LSCC specimens and 10 vocal cord polyp specimens were detected by immunohistochemistry. The correlation between the expression of hTERT protein and c-myc protein and their relationship with clinicopathological parameter were analyzed.Result: The positive rate of hTERT protein and c-myc protein expression in 39 LSCC was 84.6% (33/39) and 82.1% (32/39), respectively. The positive rate of hTERT protein and c-myc protein expression in 10 vocal cord polyps was 0 (0/10) and 10.0% (1/10) respectively. Statistical tests showed that hTERT and c-myc protein expression was significantly higher in LSCC than that in vocal cord polyps. Spearman rank correlation analysis revealed that the expression of c-myc protein was great significantly related to that of hTERT protein (correlation coefficient r = 0.541, P < 0.01). No statistically association was observed among the frequency of hTERT expression and TNM stages, degree of differentiation or lymph nodes metastases.Conclusion: The activation of hTERT may be a key step in the pathogenesis of LSCC and the expression of hTERT was associated significantly with that of c-myc gene. C-myc is probably an important control factor in the activation and regulation of hTERT expression.","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 21","pages":"963-6"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26544393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Quantitative evaluation of the focus range of juvenile onset recurrent respiratory papillomatosis]. [少年起病复发性呼吸道乳头状瘤病病灶范围的定量评价]。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Wenbin Lei, Zhenzhong Su, Weiping Wen, Liping Chai, Aiyun Jiang, Guangyu Luo, Jian Li

{"title":"[Quantitative evaluation of the focus range of juvenile onset recurrent respiratory papillomatosis].","authors":"Wenbin Lei, Zhenzhong Su, Weiping Wen, Liping Chai, Aiyun Jiang, Guangyu Luo, Jian Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the clinical application of quantitative evaluation of the focus range of juvenile onset recurrent respiratory papillomatosis (JO-RRP).Method: Disease severity was scored as the sum of ratings of 1 (minimal), 2 (moderate), or 3 (severe) for involvement of 22 areas of the respiratory tract and digestive tract. Sixty-eight cases with JO-RRP patients underwent 238 procedures were removed papillomas. A Storz laryngoscope was used to exposed and suspend the larynx, and an endoscope video system was used to display the pathological changes.Result: The score of these cases range from 2 to 20, most of them range from 7 to 14. Papillomas were found in the larynx in each case, vocal cords and false vocal cords were the most common places involved. Nose, pharynx, hard palate, lung or esophagus involved was seldom found. The score declined as the patients underwent more procedures. It is in accord with the clinical characteristic of JO-RRP.Conclusion: We concluded that our means of JO-RRP focus range quantitative evaluation could evaluate the severity and clinical curative effect objectively. It was a scientific and convenient way, and worth to be generalized.","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 21","pages":"970-2"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26544395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The value of spiral CT scan to diagnose the respiratory tract foreign body with negative chest X-rays]. 【螺旋CT扫描对胸部x线阴性呼吸道异物的诊断价值】。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Huiteng Wang, Zhinan Wang, Jianbo Shao

引用次数: 0

[Digital recording and equivalent evaluation of Mandarin speech test materials]. [普通话语音测试材料的数字记录与等效评价]。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Hua Zhang, Shuo Wang, Liang Wang, Jing Chen, Pengpeng Yue, Liansheng Guo, Xiaoyan Zhao

{"title":"[Digital recording and equivalent evaluation of Mandarin speech test materials].","authors":"Hua Zhang, Shuo Wang, Liang Wang, Jing Chen, Pengpeng Yue, Liansheng Guo, Xiaoyan Zhao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: After preliminary edit and equivalent tests, Mandarin speech test materials (MSTM) were digitally recorded and further tested in order to get multi-center analysis and clinical application.Method: The contents of the lists were modified and the trial lists were added. All materials were digitally recorded into 3 CDs with international criterions. Each CD contained a calibration pure-tone, a trial list and the speech test lists. MSTM contained 10 lists of monosyllabic words with 50 words in each list, 10 lists of spondees with 50 disyllabic words (100 Chinese characters) in each lists, and 30 lists of sentences with 10 sentences in each list. Sixty normal-hearing college students were tested. The tests were conducted in the sound booths with earphones. The presentation levels of the monosyllables, disyllables and sentences were different. The presentation levels considered as the scores were about 50% correct,in which condition the speech intelligibility was most sensitive.Result: Statistic analysis showed that two sets (9 lists in each set) of monosyllable lists, 9 disyllable lists and 27 sentence lists were equivalent in difficulty. If the clinicians do not need so many lists to test the patients, they had better use the most equivalent ones.Conclusion: The equivalent Mandarin speech lists have been made into CDs and ready to be used clinically. But the multi-center evaluation is needed.","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 22","pages":"1011-5"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26519835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA]. [反义perlecan cDNA稳定表达对Hep-2细胞增殖的抑制]。

Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology Pub Date : 2006-11-01

Guangli Chen, Shusheng Gong, Juan Ding, Yingpeng Liu, Linghui Luo, Jianting Wang, Pei Chen, Kaisheng Yan

{"title":"[Suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA].","authors":"Guangli Chen, Shusheng Gong, Juan Ding, Yingpeng Liu, Linghui Luo, Jianting Wang, Pei Chen, Kaisheng Yan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA.Method: In this study, the plasmids of recombination eukaryotic expression vector perlecan anti-sense cDNA (pAP) were transfected into Hep-2 cells by using cationic liposome (lipofectamine 2000) and divided into three groups: non-transfected group, WT group; transfection with no load carrier, neo group; and transfection with the pAP plasmid, pAP group. Semi quantify RT-PCR, western blot assay and MTT assay were used to detected the expression of perlecan mRNA and protein in the three groups; the level of cell proliferation; and the responsivity of basic fibroblast growth factor (bFGF).Result: It was showed that the expression of perlecan mRNA and protein were significantly reduced in the pAP group compared with WT group and ph beta Apr-neol transfected group ( P < 0.01). In the presence of 1 microg/L of bFGF in low serum (0.1% FCS), the pAP transfected cells showed a reduced proliferation rate (MTT assay) while the wild type cells and ph beta Apr-neol transfected cells grew rapidly.Conclusion: The growth of Hep-2 cells could be inhibited significantly by perlecan anti-sense cDNA plasmids transfection.","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 21","pages":"984-7"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26544903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0