{"title":"[Expression of mACh receptor ml-m5 subunits in the vestibular endorgans of rat].","authors":"Huanmao Cheng, Weijia Kong, Qi Yao","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.</p><p><strong>Method: </strong>Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.</p><p><strong>Result: </strong>It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.</p><p><strong>Conclusion: </strong>Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.</p>","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 22","pages":"1027-9"},"PeriodicalIF":0.0000,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.
Method: Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.
Result: It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.
Conclusion: Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.
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