Annales de biologie clinique最新文献_第5页

Antinuclear antibodies in children: indirect immunofluorescence patterns, antigen targets and associated diagnoses 儿童抗核抗体:间接免疫荧光模式、抗原靶点和相关诊断

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-03-15 DOI: 10.1684/abc.2023.1777

Hind Zrikem, Fatima E Eddebhi, Raja Hazime, Brahim Admou

{"title":"Antinuclear antibodies in children: indirect immunofluorescence patterns, antigen targets and associated diagnoses","authors":"Hind Zrikem, Fatima E Eddebhi, Raja Hazime, Brahim Admou","doi":"10.1684/abc.2023.1777","DOIUrl":"https://doi.org/10.1684/abc.2023.1777","url":null,"abstract":"Antinuclear antibodies tests are of a paramount importance in the diagnosis, classification, prognostic evaluation and management of autoimmune diseases in children. The present study aimed to describe the immuno-clinical profile of antinuclear antibodies tests in a pediatric population in order to guide the clinical practice of biologists and clinicians. Our study enrolled 268 children. Antinuclear antibodies screening was performed using the indirect immunofluorescence assay on HEp-2 cells. Identification of target antigens was conducted using separately or at one timepoint the following techniques: enzyme-linked immunosorbent assay, Immunodot and Chemiluminescence. The average age of patients was 9.6 ± 4.3 years, with a female predominance (sex-ratio = 1.9). Antinuclear antibodies screening was positive in 40.67% of cases. The most frequently observed antinuclear antibodies patterns were speckled (52.3%), homogeneous (13.8%) and mixed homogeneous-speckled (13.8%). Autoantibodies were detected in 4 patients (2.51%) for whom ANA testing using the indirect immunofluorescence assay was negative. Positive antinuclear antibodies specificities were detected in connective tissue diseases (44.03%; n = 48), organ-specific autoimmune diseases (10.09%; n = 11), and in non-autoimmune conditions (inflammatory diseases, infections, hematological diseases, vasculitis and Wilson’s disease) (32.08%; n = 35). Our study revealed a high rate of positive antinuclear antibodies tests in the pediatric population, mainly related to autoimmune diseases (54.12%) besides non-autoimmune conditions (32.08%). Therefore, screening and interpretation of antinuclear antibodies testing in children require the consideration of clinical data and a close collaboration between clinicians and biologists.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9120136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Achieve our ambitions 实现我们的抱负

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-03-15 DOI: 10.1684/abc.2023.1778

Katell Peoc'h

引用次数: 0

Remerciements. 致谢。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-02-01 DOI: 10.1684/abc.2023.1793

引用次数: 0

Evolution of attractiveness of Medical Biology to medical students in 2022 2022年医学生物学对医学生吸引力的演变

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1769

Marouan Bennani, Lionel Barrand, Florence Guillotin, Grégory Thomson, Carole Poupon, Kévin Cassinari

引用次数: 0

Medical biology in France: evolution and issues 法国的医学生物学:进化和问题

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1775

Dominique Bonnefont-Rousselot, Marc Delpech, Philippe Chatron, Jean-Louis Gueant, Yves Le Bouc, François-Xavier Maquart, Bernard Massoubre, Nathalie Rives, Claude Vigneron

{"title":"Medical biology in France: evolution and issues","authors":"Dominique Bonnefont-Rousselot, Marc Delpech, Philippe Chatron, Jean-Louis Gueant, Yves Le Bouc, François-Xavier Maquart, Bernard Massoubre, Nathalie Rives, Claude Vigneron","doi":"10.1684/abc.2022.1775","DOIUrl":"https://doi.org/10.1684/abc.2022.1775","url":null,"abstract":"Medical biology is an essential part of patient care, both for the diagnosis and monitoring of diseases and for certain therapeutic advances. However, in recent years, it has been confronted with fundamental questions concerning its future. This report is the follow-up to the one published in 2018 by the National Academies of Medicine and Pharmacy and unfortunately only confirms a strong deterioration at all levels. The public authorities do not assume their role of regulator, thus allowing the excessive financialization of Medical Biology to grow considerably and lead to disproportionate groupings of Medical Biology Laboratories (MBL), destructive and sources of health risks. The result is that the Medical Biology Laboratories in towns, which are already known to be poorly distributed, are gradually becoming simple sampling sites, with patients finding themselves alone, often anxious, with their results sent to them by Internet without interpretation. Moreover, although progress in the field of Medical Biology is incredible and should constitute a major pole of attraction for young people, the disaffection of the discipline is total and worrying. Finally, innovation, in the context of current technological progress: connected devices, artificial intelligence and big data, represents a major challenge for the future. Here again, little or nothing is being done, even though the challenges are immense. After these alarming observations, the report will end with a series of recommendations aimed at optimizing the entry of MBL into a new era.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methemoglobin poisoning and interference on Sysmex® XN apparatus 高铁血红蛋白中毒及对Sysmex®XN仪器的干扰

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1772

Benjamin Podvin, Elise Fournier, Quentin Bigourd, Jean-Maxime Devaux, Adélaïde Hardeman

引用次数: 0

Prevalence of microbial nosocomial infections in the resuscitation unit of the University Hospital of Annaba-Algeria 阿尔及利亚安纳巴大学医院复苏病房微生物医院感染的流行情况

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1766

Chahrazed Benzaid, Lazhari Tichati, Mahmoud Rouabhia, Sonia Akil Dahdouh

{"title":"Prevalence of microbial nosocomial infections in the resuscitation unit of the University Hospital of Annaba-Algeria","authors":"Chahrazed Benzaid, Lazhari Tichati, Mahmoud Rouabhia, Sonia Akil Dahdouh","doi":"10.1684/abc.2022.1766","DOIUrl":"https://doi.org/10.1684/abc.2022.1766","url":null,"abstract":"Nosocomial infections constitute a significant public health problem but are poorly controlled in our health structures, especially those associated with resuscitation care. The first objective of this study was to identify the different microbial strains present in different biological samples taken from patients staying in the resuscitation unit of the Annaba University Hospital Center. The second objective was to assess the antimicrobial sensitivity of isolated microbes from the patients’ samples, to determine the risk factors, the most incriminated microbial agents in nosocomial infections. During the study period from January 2013 to December 2016, we collected 1,151 biological samples from 1,938 patients admitted to Resuscitation Medical Service. The samples were subjected to different microbiological analyses. Our results showed that over 59% of the collected samples were microbiologically positive. The identified species include Candida albicans (115 cases) and Candida.sp (81 cases). The Gram-negative bacterial strains found in the samples included Acinetobacter baumannii (108 cases), Klebssiella pneumoniae (99 cases) Pseudomonas aeruginosa (79 cases), and Escherichia coli (73 cases). Gram positive bacteria included Staphylococcus aureus (94 cases) and Enterococcus faecalis (53 cases). The antibiogram analyses showed significant antibiotic resistance reaching 93.75% for ampicillin, but sensitivity to colistin reaching 81.81%. Moreover, the fungal strains are represented by the genus albicans, showing a significant resistance to antifungals, reaching 80% with miconazole. Conclusion. The nosocomial infections in the medical unit were caused by the candida genus and multi-resistant bacteria to various antibiotics and antifungals. The most important factor associated with these infections was the use of medical devices.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10621887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of blood gas results obtained on Abbott i-Stat® and on Radiometer ABL 800 Flex® analyzers Impact for the clinical decision 雅培i-Stat®和Radiometer ABL 800 Flex®血气分析仪血气结果的比较对临床决策的影响

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1765

Romain Jouffroy, Maude Laney, Teddy Leguillier, Valérie Nivet-Antoine, Jean-Louis Beaudeux

{"title":"Comparison of blood gas results obtained on Abbott i-Stat® and on Radiometer ABL 800 Flex® analyzers Impact for the clinical decision","authors":"Romain Jouffroy, Maude Laney, Teddy Leguillier, Valérie Nivet-Antoine, Jean-Louis Beaudeux","doi":"10.1684/abc.2022.1765","DOIUrl":"https://doi.org/10.1684/abc.2022.1765","url":null,"abstract":"Background: Point of care testing (POCT) tests are needed to assess severity and to help for triage in hospital and in prehospital settings. Before their use, the analytical performances of POCTs have to be compared with central laboratory reference methods. In this study, we describe the comparability of results obtained by either the Abbott i-STAT® System POCT handheld device or the blood gases analyzer of the central laboratory of our hospital.Methods: Sample blood from 37 septic patients admitted to the intensive care unit (ICU) were assayed by Abbott i-STAT® System POCT and Radiometer ABL800 Flex® lab analyzer. Studied parameters were as follows: pH, pO2, pCO2, base excess (BE), HCO3- and lactate. Comparability was evaluated using Bland-Altman method. The clinical value for possible mismatch issued of values differences was also assessed.Results: Quite acceptable correlations in results of POCT and laboratory analyzer were observed with R² most of time above 0.85. Bland-Altman analysis showed a bias of 1.26% for Abbott i-STAT® System POCT vs laboratory.Conclusion: Abbott i-STAT® System POCT handheld device is comparable to Radiometer ABL800 Flex® lab analyzer and concordant with laboratory analysis. Abbott i-STAT® System POCT handled device could be used in the prehospital settings in order to evaluate the severity of sepsis.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10621885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of two SARS-CoV-2 RT-PCR assays and implication of the instrument software on cycle threshold (Ct) value 两种SARS-CoV-2 RT-PCR检测方法的比较及仪器软件对周期阈值(Ct)的影响

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1771

Eric Farfour, Tiffany Pascreau, Sara Zia-Chahabi, Émilie Jolly, Patrice Ferrière, Laurence Mazaux, Marc Vasse

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Lu pour vous dans…. 为你读…

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2022-11-01 DOI: 10.1684/abc.2022.1776

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