Comparison of two SARS-CoV-2 RT-PCR assays and implication of the instrument software on cycle threshold (Ct) value

IF 0.4 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Eric Farfour, Tiffany Pascreau, Sara Zia-Chahabi, Émilie Jolly, Patrice Ferrière, Laurence Mazaux, Marc Vasse
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引用次数: 0

Abstract

The Cycle Threshold (Ct) value of SARS-CoV-2 RT-PCR are used as an indicator of viral load. Using a collection of 45 fresh nasopharyngeal samples collected on universal transport media, we compare the Ct value obtained with 2 RT-PCR assays, the Alinity M SARS-CoV-2 and the Alinity M RESP-4-Plex (Abbott Molecular, Des Plaines, Illinois, Etats-Unis) processed on an Alinity M device. The assays are highly correlated; however, the Ct values were in median lower of 4.54 with the Alinity M RESP-4-Plex. This difference could be attributed to earlier detection of positivity by the software of the Alinity M rather than a difference in RT-PCR performances. The Ct-value of SARS-CoV-2 RT-PCR should be interpreted with caution taking into account the clinical context, pre-analytical and analytical findings.

两种SARS-CoV-2 RT-PCR检测方法的比较及仪器软件对周期阈值(Ct)的影响
使用SARS-CoV-2 RT-PCR的周期阈值(Ct)值作为病毒载量的指标。使用通用运输介质收集的45份新鲜鼻咽样本,我们比较了在Alinity M设备上处理的Alinity M SARS-CoV-2和Alinity M respr -4- plex (Abbott Molecular, Des Plaines, Illinois, etats - unitis)的2种RT-PCR检测获得的Ct值。分析结果高度相关;而Alinity M RESP-4-Plex的Ct值中位数较低,为4.54。这种差异可能归因于Alinity M软件更早地检测到阳性,而不是RT-PCR性能的差异。考虑到临床背景、分析前和分析结果,应谨慎解释SARS-CoV-2 RT-PCR的ct值。
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来源期刊
Annales de biologie clinique
Annales de biologie clinique 医学-医学:研究与实验
CiteScore
0.80
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Multidisciplinary information with direct relevance to everyday practice Annales de Biologie Clinique, the official journal of the French Society of Clinical Biology (SFBC), supports biologists in areas including continuing education, laboratory accreditation and technique validation. With original articles, abstracts and accounts of everyday practice, the journal provides details of advances in knowledge, techniques and equipment, as well as a forum for discussion open to the entire community.
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