Annales de biologie clinique最新文献

[BIOMED-J 2023 award-winning poster abstracts]. [BIOMED-J 2023获奖海报摘要]。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1801

Mounia Allal-Elasmi, Nûn Bentounes, Valery Brunel, Maëlle Gallin, Mariem Gazzeh, Élodie Lebredonchel, Maya Nechar, Hichem Assami

引用次数: 0

[Atlas of flow cytometry images: lymphoproliferative syndromes - Additional images obtained with DxFlex]. [流式细胞术图像图谱：淋巴增生综合征-使用DxFlex获得的其他图像]。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1817

Véronique Harrivel, Caroline Mayeur-Rousse, Sabrina Bouyer, Lucile Baseggio

引用次数: 0

[Impact of voxelotor on hemoglobin electrophoretic and chromatographic profiles]. [voxelotor对血红蛋白电泳和色谱谱的影响]。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1814

Soraya Fellahi, Nadir Mouri, Baptiste Giraud, Suella Martino, Gonzalo de Luna, Mehdi Sakka, Philippe Joly, Jean-Philippe Bastard, Frédéric Galacteros, Stéphane Moutereau

{"title":"[Impact of voxelotor on hemoglobin electrophoretic and chromatographic profiles].","authors":"Soraya Fellahi, Nadir Mouri, Baptiste Giraud, Suella Martino, Gonzalo de Luna, Mehdi Sakka, Philippe Joly, Jean-Philippe Bastard, Frédéric Galacteros, Stéphane Moutereau","doi":"10.1684/abc.2023.1814","DOIUrl":"https://doi.org/10.1684/abc.2023.1814","url":null,"abstract":"Voxelotor (GBT440, OXBRYTA®) appeared recently as one of the possible treatments for sickle cell disease. This molecule, by binding the alpha globin of hemoglobin, causes hyperaffinity of the latter for oxygen and reduces its polymerization properties. Several therapeutic trials have been able to show its effectiveness on certain aspects of sickle cell disease; thus, the french HAS (High Authority of Health) college issued an early access authorization and, since 2021, this treatment can be offered to patients under a temporary authorization for use. Consequently, the laboratories that carry out the biological monitoring of sickle cell patients will be confronted with new profiles characteristic of the presence of hemoglobin combined with GBT440. This work presents a collection of images obtained by different techniques: HPLC, capillary electrophoresis, isoelectrofocusing, alkaline gel and acid agar gel electrophoresis in transfused or non-transfused sickle cell disease patients. The ability to observe the presence of GBT440 by these analyzes could be useful in order to characterize the therapeutic follow-up of patients.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":"81 3","pages":"320-326"},"PeriodicalIF":0.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10215158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Use of heparin calibrated anti-Xa assay for apixaban and rivaroxaban measurement in the context of regional telestroke activity]. [使用肝素校准抗xa测定阿哌沙班和利伐沙班在区域卒中活动的背景下测量]。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1812

Céline Delassasseigne, Clémentine Leroux, Pauline Renou, David Girard, Anne Lafargue, Stéphane Morel, Pierre Thomas Belotti, Laurent Weinmann

{"title":"[Use of heparin calibrated anti-Xa assay for apixaban and rivaroxaban measurement in the context of regional telestroke activity].","authors":"Céline Delassasseigne, Clémentine Leroux, Pauline Renou, David Girard, Anne Lafargue, Stéphane Morel, Pierre Thomas Belotti, Laurent Weinmann","doi":"10.1684/abc.2023.1812","DOIUrl":"https://doi.org/10.1684/abc.2023.1812","url":null,"abstract":"Introduction: In Bordeaux University Hospital, neurologists are required to prescribe thrombolysis using telemedicine (telethrombolysis) for anticoagulated stroke patients admitted in peripheral centers in the Nouvelle-Aquitaine region. However, due to the bleeding risk, the maximum concentration of DOAC authorizing thrombolysis is 30, 50 or 100 ng/mL (depending on the sources and the patient-specific benefit-risk ratio). Most of the time, specific assays of Direct Oral Anticoagulants (DOACs) are not available in these peripheral centers. We therefore studied an alternative test: the Unfractionated Heparin (UFH) anti-Xa activity which is available in most laboratories and could be used to estimate the DOAC concentration.Methods: Five centers were included in our study: three centers using the Liquid Anti-Xa HemosIL® Werfen reagent and two centers using the STA-Liquid Anti-Xa® Stago reagent. For each reagent, we established correlation curves between DOAC and UFH anti-Xa activities and determinated UFH cut-offs for the thresholds of 30, 50 and 100 ng/mL respectively.Results: A total of 1455 plasmas were tested. There is an excellent correlation between DOAC and UFH anti-Xa activities using a third-degree modeling curve, independently the reagent used. However, a significant inter-reagent variability is observed concerning the obtained cut-offs.Conclusion: Our study makes unsuitable the use of a universal cut-off. In opposition to recommendations made by other publications, the UFH cut-offs must be adapted to the reagent used locally by the laboratory, and to the considered DOAC.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":"81 3","pages":"269-279"},"PeriodicalIF":0.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9856236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of the reticulocyte hemoglobin equivalent in the exclusion of latent iron deficiency. 网织红细胞血红蛋白当量在排除潜伏性缺铁中的重要性。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1811

Ghada Bouzid, Nada Yousfi, Zeineb Ben Hassine, Foued Daly, Amal Attoini, Nawel Daoud, Souheil Omar, Naouel Ben Salah

{"title":"Importance of the reticulocyte hemoglobin equivalent in the exclusion of latent iron deficiency.","authors":"Ghada Bouzid, Nada Yousfi, Zeineb Ben Hassine, Foued Daly, Amal Attoini, Nawel Daoud, Souheil Omar, Naouel Ben Salah","doi":"10.1684/abc.2023.1811","DOIUrl":"https://doi.org/10.1684/abc.2023.1811","url":null,"abstract":"Background: Iron deficiency is an underdiagnosed public health problem, especially in developing countries, that can conceal serious underlying illnesses. Early diagnosis and treatment of latent iron deficiency (LID) is crucial. Reticulocyte hemoglobin equivalent (RET-He), was reported to be a cost-effective tool that reflects the iron availability at erythropoiesis. The aims of this study were to evaluate the RET-He in the exclusion of LID.Methods: Transversal study was carried out in the laboratory of clinical biology of Ben Arous regional hospital, it included volunteers in apparently good health. We performed a complete blood count and a serum ferritin assay. Participants with normal hemoglobin were divided into two groups: Control group G1: normal ferritin (≥ 15 ng/mL)/LID group G2: low ferritin (< 15 ng/mL). We compared the blood count parameters of the two groups.Results: We selected 108 participants (G1: 88 (81.5%), G2: 20 (18.5%)), mean age = 36 years, gender-ratio = 0.92. We noted, in G2, significantly lower rates for hemoglobin Hb (p < 0.001), hematocrit (p < 0.001), mean corpuscular hemoglobin MCH (p = 0.026), reticulocyte count (p = 0.039) and RET-He (p < 0.001) and significantly higher rate for RDW/CV (p = 0.009). RET-He averages were 29.1 pg in G2 and 31.1pg in G1. In multivariate analysis, only RET-He showed a significant difference between the two groups. Area under the curve was 0.872, the cutoff = 30.9 (sensitivity 100%, specificity 61%, PPV 37%, NPV 100%).Conclusion: RET-He is an accessible and affordable parameter of the iron status, with an excellent NPV. It would be interesting to evaluate our results on a larger sample to define reference values in our population.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":"81 3","pages":"262-268"},"PeriodicalIF":0.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9856239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison between radiometry and spectrophotometry for the determination of angiotensin-converting enzyme activity in cerebrospinal fluid. 放射测量法和分光光度法测定脑脊液中血管紧张素转换酶活性的比较。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1809

Ludmia Taibi, Bénédicte Bénéteau-Burnat, Michel Vaubourdolle, Bruno Baudin

{"title":"Comparison between radiometry and spectrophotometry for the determination of angiotensin-converting enzyme activity in cerebrospinal fluid.","authors":"Ludmia Taibi, Bénédicte Bénéteau-Burnat, Michel Vaubourdolle, Bruno Baudin","doi":"10.1684/abc.2023.1809","DOIUrl":"10.1684/abc.2023.1809","url":null,"abstract":"Determination of angiotensin-converting enzyme (ACE) activity in cerebrospinal fluid (CSF) can help for establishing the diagnosis of neurosarcoidosis. We investigated the performance characteristics of two assays for ACE determination in 57 CSF, radiometry with [glycine-1-14C] benzoyl-L-histidyl-L-leucine and spectrophotometry with furylacryloyl-phenylalanyl-L-glycyl-L-glycine (FAPGG) as substrates. We compared both kinetic assays to an ELISA specific for human ACE. Within run and between run imprecisions were 14-17% for radiometry, 6-19% for spectrophotometry and 5-8% for ELISA. The limit of detection was 0.04 U/L for radiometry, 1.0 U/L for spectrophotometry and 0.156 μg/L for ELISA. The limit of quantification was 0.06 U/L for radiometry, 1.5 U/L for spectrophotometry, but not known for ELISA. The domain for quantification was 0.06-4.0 U/L for radiometry, 1.5-24 U/L for spectrophotometry and 0.156-10 μg/L for ELISA. Deming regression and Bland-Altman plots show good correlations between the three assays, but with high slopes, because both kinetic assays use different substrates and ELISA measures ACE molecule but not activity. Radiometry was more sensitive than spectrophotometry, which has a limit of detection above most pathological levels. ELISA could be an alternative to radiometry but only after complete evaluation, determination of normal values and assessment of its clinical value. We claim for standardization of ACE determination as well as in serum as in other biological fluids, in particular CSF.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":"81 3","pages":"255-261"},"PeriodicalIF":0.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10200576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[Advice in autoimmunity biological diagnosis: guidelines for drafting comments on biological results]. [在自身免疫生物学诊断方面的建议:生物学结果评论意见起草指南]。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1808

Marie Sénant, Marie-Agnès Dragon-Durey, Chantal Dumestre-Perard, Lucile Musset, Laurence Guis-Cabanne, Marie-Alexandra Alyanakian, Yves Allenbach, David Goncalves, Nicole Fabien

引用次数: 0

[31^st National Days of the National College of Hospital Biochemistry]. 【国立医院生物化学学院第31个国庆日】。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1813

François Schmitt, Isabelle Tostivint, Vincent Frochot, Laurence Piéroni, Sylvie Caspar-Bauguil, Maëlle Plawecki, Martine Roubille, Régine Cartier, Matthieu Pecquet

引用次数: 0

[Contribution of cytogenetic in the diagnosis of Edwards's syndrome: about 9 cases]. 细胞遗传学在爱德华兹综合征诊断中的贡献:约9例。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1816

Fatima Ezzahra Aouni, Khawla Zerrouki, Fatimazahra Smaili, Anass Ayyad, Sahar Messaoudi, Abdeladim Babakhouya, Rim Amrani, Mariam Tajir

{"title":"[Contribution of cytogenetic in the diagnosis of Edwards's syndrome: about 9 cases].","authors":"Fatima Ezzahra Aouni, Khawla Zerrouki, Fatimazahra Smaili, Anass Ayyad, Sahar Messaoudi, Abdeladim Babakhouya, Rim Amrani, Mariam Tajir","doi":"10.1684/abc.2023.1816","DOIUrl":"https://doi.org/10.1684/abc.2023.1816","url":null,"abstract":"Introduction: Trisomy 18 is a constitutional chromosomal disorder defined by the presence of a supernumerary chromosome 18. The diagnosis is suspected clinically and confirmed by cytogenetic analysis. Genetic counseling for patients' families is important. The objective of this study is to report our experience in Medical Genetics Department at the Mohammed VI University Hospital of Oujda in the diagnosis and genetic counseling of trisomy 18 through dysmorphological expertise and cytogenetic analysis.Material and methods: We report a retrospective descriptive study over a period of four years (2018-2022) of nine patients with polymalformative syndrome suggestive of trisomy 18 who underwent cytogenetic analysis.Results: The median age of patients at diagnosis was 2 days with a male predominance. The mean maternal age at birth of the patients in our series was 40 years. Consanguinity was found in only one patient. All patients had a typical phenotype of trisomy 18. The postnatal constitutional karyotype showed a homogeneous trisomy 18 in all patients. In our series, only one patient is still alive at the age of 7 months, the other 8 patients died with a median postnatal survival of 5 days.Conclusion: We underline through this study, the contribution of the medical geneticist in the clinic and cytogenetic diagnostic approach of rare chromosomal affections, in order to provide an adequate genetic counseling to the families.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":"81 3","pages":"304-309"},"PeriodicalIF":0.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10215159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation of a diagnostic test for heparin-induced thrombocytopenia: validation of a delayed test time]. [评价肝素诱发的血小板减少症的诊断试验:延迟试验时间的验证]。

IF 0.5 4区医学

Annales de biologie clinique Pub Date : 2023-07-21 DOI: 10.1684/abc.2023.1810

Paul Billoir, Virginie Barbay, Marielle Fresel, Sabine Brunel, Véronique Le Cam Duchez

{"title":"[Evaluation of a diagnostic test for heparin-induced thrombocytopenia: validation of a delayed test time].","authors":"Paul Billoir, Virginie Barbay, Marielle Fresel, Sabine Brunel, Véronique Le Cam Duchez","doi":"10.1684/abc.2023.1810","DOIUrl":"https://doi.org/10.1684/abc.2023.1810","url":null,"abstract":"Heparin-induced thrombocytopenia (HIT) is a rare, iatrogenic condition, characterized by its potential severity and diagnostic difficulties. The diagnosis is based on a set of arguments allowing the calculation of a pre-test score pointing to HIT. There are rapid diagnostic tests for suspected HIT. Among these, the STic Expert® HIT has a good sensitivity to detect HIT. However, it must be performed within 2 hours after sampling. The aim of this study was to evaluate a delayed STic Expert® HIT test at 8 hours and in frozen plasma. Thirty-six patients were prospectively included for HIT testing between April 01, 2018, and July 1, 2022, at the University Rouen Hospital. For any request for HIT testing, an analysis by STic Expert® HIT was performed within 2 hours and 8 hours post-sampling. Any positive result was confirmed by a functional test, platelet aggregation with heparin, release of 14C-serotonin assay (SRA), and immunological assay by a research for anti-platelet factor 4 IgG antibodies. Twenty-three patients had a STic Expert® HIT. Sixteen presented platelet aggregations in the presence of heparin and had a positive anti-PF4 test, 17 had a positive SRA. Six patients had no HIT. For the test performed within 2 hours of collection, the Se = 100%, Sp = 68.42%, PPV = 73.91%, and NPV = 100%. The X2 = 18.21 with p < 0.001. For the test performed at 8 hours post sampling, the Se = 100%, Sp = 68.42%, PPV = 73.91% and NPV = 100%. The X2 = 18.21 with p < 0.001. In conclusion, we have demonstrated that the STic Expert® can be used to perform an HIT diagnostic test 8 hours after sampling and on thawed plasma. However, this study needs to be confirmed on a larger number of samples.","PeriodicalId":7892,"journal":{"name":"Annales de biologie clinique","volume":"81 3","pages":"248-254"},"PeriodicalIF":0.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9856240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0