Comparison between radiometry and spectrophotometry for the determination of angiotensin-converting enzyme activity in cerebrospinal fluid.

IF 0.4 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Ludmia Taibi, Bénédicte Bénéteau-Burnat, Michel Vaubourdolle, Bruno Baudin
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引用次数: 1

Abstract

Determination of angiotensin-converting enzyme (ACE) activity in cerebrospinal fluid (CSF) can help for establishing the diagnosis of neurosarcoidosis. We investigated the performance characteristics of two assays for ACE determination in 57 CSF, radiometry with [glycine-1-14C] benzoyl-L-histidyl-L-leucine and spectrophotometry with furylacryloyl-phenylalanyl-L-glycyl-L-glycine (FAPGG) as substrates. We compared both kinetic assays to an ELISA specific for human ACE. Within run and between run imprecisions were 14-17% for radiometry, 6-19% for spectrophotometry and 5-8% for ELISA. The limit of detection was 0.04 U/L for radiometry, 1.0 U/L for spectrophotometry and 0.156 μg/L for ELISA. The limit of quantification was 0.06 U/L for radiometry, 1.5 U/L for spectrophotometry, but not known for ELISA. The domain for quantification was 0.06-4.0 U/L for radiometry, 1.5-24 U/L for spectrophotometry and 0.156-10 μg/L for ELISA. Deming regression and Bland-Altman plots show good correlations between the three assays, but with high slopes, because both kinetic assays use different substrates and ELISA measures ACE molecule but not activity. Radiometry was more sensitive than spectrophotometry, which has a limit of detection above most pathological levels. ELISA could be an alternative to radiometry but only after complete evaluation, determination of normal values and assessment of its clinical value. We claim for standardization of ACE determination as well as in serum as in other biological fluids, in particular CSF.

放射测量法和分光光度法测定脑脊液中血管紧张素转换酶活性的比较。
脑脊液中血管紧张素转换酶(ACE)活性的测定有助于神经肉瘤的诊断。我们研究了两种测定57 CSF中ACE的方法的性能特征,一种是[甘氨酸1-14C]苯甲酰基-L-组氨酸-L-亮氨酸的放射测定法,另一种是以糠丙烯酰基-苯丙氨酸基-L-甘氨酰基-L-甘氨酸(FAPGG)为底物的分光光度法。我们将两种动力学测定与人ACE特异性ELISA进行了比较。放射测量法的批内和批间不精确性为14-17%,分光光度法为6-19%,ELISA为5-8%。放射测定法的检出限为0.04U/L,分光光度法的检出极限为1.0U/L,ELISA法的检出限度为0.156μg/L。放射测量法的定量限为0.06 U/L,分光光度法的定量极限为1.5 U/L,但ELISA的定量限未知。放射测定法的定量范围为0.06-4.0U/L,分光光度法的定量域为1.5-24U/L,ELISA的定量域则为0.156-10μg/L。Deming回归和Bland-Altman图显示了三种测定之间的良好相关性,但斜率很高,因为两种动力学测定都使用不同的底物,ELISA测量ACE分子,但不测量活性。放射测量法比分光光度法更灵敏,分光光度计的检测极限高于大多数病理水平。ELISA可以替代放射测量法,但必须经过全面评估、正常值测定和临床价值评估。我们主张ACE测定的标准化,以及血清和其他生物流体,特别是CSF中的标准化。
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来源期刊
Annales de biologie clinique
Annales de biologie clinique 医学-医学:研究与实验
CiteScore
0.80
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Multidisciplinary information with direct relevance to everyday practice Annales de Biologie Clinique, the official journal of the French Society of Clinical Biology (SFBC), supports biologists in areas including continuing education, laboratory accreditation and technique validation. With original articles, abstracts and accounts of everyday practice, the journal provides details of advances in knowledge, techniques and equipment, as well as a forum for discussion open to the entire community.
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