Anatomia最新文献_第7页

Detection of Potential Markers for Lip Vermilion Epithelium in Japanese Macaques Based on the Results of Gene Expression Profile 基于基因表达谱结果的日本猕猴唇朱红色上皮潜在标记物检测

Anatomia Pub Date : 2022-01-24 DOI: 10.3390/anatomia1010002

H. Kato, Yiwei Ling, Emi Hoshikawa, A. Suzuki, K. Haga, E. Naito, A. Uenoyama, Shujiro Okuda, K. Izumi

{"title":"Detection of Potential Markers for Lip Vermilion Epithelium in Japanese Macaques Based on the Results of Gene Expression Profile","authors":"H. Kato, Yiwei Ling, Emi Hoshikawa, A. Suzuki, K. Haga, E. Naito, A. Uenoyama, Shujiro Okuda, K. Izumi","doi":"10.3390/anatomia1010002","DOIUrl":"https://doi.org/10.3390/anatomia1010002","url":null,"abstract":"Development of effective in vitro human lip models, specific to the vermilion epithelium, has not progressed as much as that of skin and oral mucosa/gingiva models in vitro. Our histologic examination demonstrated that a Japanese macaque (male, 7 years and 9 months old) had vermilion in the lip distinct from adjacent skin and oral mucosa, resembling histological characteristics of the human lip. Therefore, in this study, we examined the gene expression profile of the three distinct epithelia (skin/vermilion/oral mucosa) within the lip of a Japanese macaque to explore a single potential marker of human vermilion epithelium. Six pairwise comparisons in the skin/vermilion/oral mucosa epithelium in vitro and in vivo revealed 69 differentially up-regulated genes in vermilion epithelium in vivo, in which a few unique genes were highly expressed when compared with both skin and oral mucosa epithelium in vivo using clustering analysis. However, we could not detect a single marker specific to vermilion epithelium supported by the gene expression profile of a Japanese macaque. Instead, the pair of keratin 10 and small proline-rich protein 3 resulted in a potential marker of vermilion epithelium in the human lip (female, 53-year-old) via a double-immunostaining technique. Nonetheless, our result may provide further clues leading to other potential markers of the vermilion epithelium.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"209 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85635057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Welcome to Anatomia: A New Open Access Journal 欢迎来到解剖学:一个新的开放获取期刊

Anatomia Pub Date : 2021-12-20 DOI: 10.3390/anatomia1010001

Francesco Fornai

引用次数: 1

Coloring plastinated specimens 着色塑化标本

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12506

S. Iliff, I. Concha, Vladimir Chereminskiy, R. Henry

引用次数: 3

Room temperature/Corcoran/Dow Corning™—Silicone plastination process 室温/Corcoran/道康宁™-硅胶塑化工艺

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12505

D. Starchik, R. Henry

{"title":"Room temperature/Corcoran/Dow Corning™—Silicone plastination process","authors":"D. Starchik, R. Henry","doi":"10.1111/ahe.12505","DOIUrl":"https://doi.org/10.1111/ahe.12505","url":null,"abstract":"For 20 years, the cold temperature/S10/von Hagens' plastination technique was used to preserve biological specimens without challenge. It became the “gold standard” for preservation of beautiful, dry biological specimens. Near the end of the 21st century, a group from the University of Michigan and environs and Dow Corning™, USA, combined silicone ingredients, similar to the von Hagens' plastination products, however in a different sequence. The new polymer (Cor‐tech) was combined with the cross‐linker to design the “impregnation mix” which would invade the cellular structure of the specimen and yet was stable at room temperature. Later, curing would be by application of the catalyst onto the impregnated specimen. This unique sequencing of products would become the “Room temperature/Dow Corning™/Corcoran—Silicone plastination technique.” The results of this room temperature technique provided similar plastinates, beautiful and practical for demonstration, containing no toxic chemical residues and forever preserved. As the name implies, impregnation of this silicone mix could be done at room temperature, without having to be kept cold. Both processes (cold and room temperature) required the same four basic steps for plastination. As well, both processes used similar basic polymers and additives to produce plastinates. However, they were combined in a different sequence. Cold temperature combines polymer and catalyst/chain extender, which is not stable and therefore must be kept colder than −15°C, while room temperature combines polymer with cross‐linker which is stable, and likely forever.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"25 1","pages":"539 - 546"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72814989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

3D Printing for veterinary anatomy: An overview 兽医解剖3D打印:概述

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12502

R. Wilhite, Inga Wölfel

引用次数: 24

Cold temperature/Biodur®/S10/von Hagens'—Silicone plastination technique 低温/Biodur®/S10/von Hagens' -硅胶塑化技术

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12472

R. Henry, G. von Hagens, G. Seamons

引用次数: 15

History and development of plastination techniques 塑化技术的历史和发展

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12497

Christoph von Horst, Rurik von Hagens, C. Sora, R. Henry

{"title":"History and development of plastination techniques","authors":"Christoph von Horst, Rurik von Hagens, C. Sora, R. Henry","doi":"10.1111/ahe.12497","DOIUrl":"https://doi.org/10.1111/ahe.12497","url":null,"abstract":"Plastination was a game‐changing invention for macroscopic anatomical preparation. The method yielded dry, odourless, tangible and durable specimens which allowed new exhibition and teaching set‐ups and paved the way for sophisticated preparations and spectacular positioning of specimens. Despite the impact of the new method, there have been similar techniques in place before. Exsiccation techniques, polymer embeddings and specimen impregnation with hardening substances were earlier methods which already included the main concepts that were later combined and refined in plastination. S10 silicone plastination, the technique most commonly known and applied, was followed by plastination methods suitable for research and sectional anatomy teaching. Numerous variations of sheet plastination techniques allow research applications and new ways of presenting topographic relations and mesoscopic insights. Besides the development of plastination techniques in sensu stricto, related techniques had a renaissance with new applications and developments, including corrosion casting and diaphonization methods. This brief review shall provide a historical context of plastination including some anecdotal spotlights on the ideas and innovations that lead to nowadays plastination techniques.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"116 1","pages":"512 - 517"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73543992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Plastination—A scientific method for teaching and research 塑化——一种科学的教学和研究方法

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12493

M. Șora, R. Latorre, C. Baptista, O. López-Albors

{"title":"Plastination—A scientific method for teaching and research","authors":"M. Șora, R. Latorre, C. Baptista, O. López-Albors","doi":"10.1111/ahe.12493","DOIUrl":"https://doi.org/10.1111/ahe.12493","url":null,"abstract":"Over the last four decades, plastination has been one of the best processes of preservation for organic tissue. In this process, water and lipids in biological tissues are replaced by polymers (silicone, epoxy, polyester) which are hardened, resulting in dry, odourless and durable specimens. Nowadays, after more than 40 years of its development, plastination is applied in more than 400 departments of anatomy, pathology, forensic sciences and biology all over the world. The most known polymers used in plastination are silicone (S10), epoxy (E12) and polyester (P40). The key element in plastination is the impregnation stage, and therefore depending on the polymer that is used, the optical quality of specimens differs. The S10 silicone technique is the most common technique used in plastination. Specimens can be used, especially in teaching, as they are easy to handle and display a realistic topography. Plastinated silicone specimens are used for displaying whole bodies, or body parts for exhibition. Transparent tissue sections, with a thickness between 1 and 4 mm, are usually produced by using epoxy (E12) or polyester (P40) polymer. These sections can be used to study both macroscopic and microscopic structures. Compared with the usual methods of dissection or corrosion, plastinated slices have the advantage of not destroying or altering the spatial relationships of structures. Plastination can be used as a teaching and research tool. Besides the teaching and scientific sector, plastination becomes a common resource for exhibitions, as worldwide more and more exhibitions use plastinated specimens.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"45 1","pages":"526 - 531"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90750705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Ultra‐thin sectioning and grinding of epoxy plastinated tissue 环氧塑化组织的超薄切片和研磨

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12478

M. Șora, Christoph von Horst, O. López-Albors, R. Latorre

{"title":"Ultra‐thin sectioning and grinding of epoxy plastinated tissue","authors":"M. Șora, Christoph von Horst, O. López-Albors, R. Latorre","doi":"10.1111/ahe.12478","DOIUrl":"https://doi.org/10.1111/ahe.12478","url":null,"abstract":"With classical sheet plastination techniques such as E12, the level and thickness of the freeze‐cut sections decide on what is visible in the final sheet plastinated sections. However, there are other plastination techniques available where we can look for specific anatomical structures through the thickness of the tissue. These techniques include sectioning and grinding of plastinated tissue blocks or thick slices. The ultra‐thin E12 technique, unlike the classic E12 technique, starts with the plastination of a large tissue block. High temperatures (30–60°C) facilitate the vacuum‐forced impregnation by decreasing the viscosity of the E12 and increasing the vapour pressure of the intermediary solvent. By sectioning the cured tissue block with a diamond band saw plastinated sections with a thickness of <300 μm can be obtained. The thickness of plastinated sections can be further reduced by grinding. Resulting sections of <100 µm are suitable for histological staining and microscopic studies. Anatomical structures of interest in thick plastinate slices can be followed by variable manual grinding in a method referred to as Tissue Tracing Technique (TTT). In addition, the tissue thickness can be adapted to the transparency or darkness of tissue types in different regions of the same plastinated section. The aim of this study was to evaluate the advantages of techniques based on sectioning and grinding of plastinated tissue (E12 ultra‐thin and TTT) compared to conventional sheet‐forming techniques (E12).","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"381 1","pages":"564 - 571"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84964647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

P40 polyester sheet plastination technique for brain and body slices: The vertical and horizontal flat chamber methods P40聚酯片脑和体片塑化技术:垂直和水平平室法

Anatomia Pub Date : 2019-11-01 DOI: 10.1111/ahe.12486

C. Baptista, K. DeJong, R. Latorre, A. S. Bittencourt

引用次数: 4