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Carbohydrate Histochemistry of the Alimentary Canal of the Shi Drum, Umbrina Cirrosa L. 石鼓植物消化道碳水化合物的组织化学研究。
Anatomia Pub Date : 2001-12-01 DOI: 10.1046/j.1439-0264.2001.00345.x
V. Pedini, P. Scocco, G. Radaelli, O. Fagioli, P. Ceccarelli
{"title":"Carbohydrate Histochemistry of the Alimentary Canal of the Shi Drum, Umbrina Cirrosa L.","authors":"V. Pedini, P. Scocco, G. Radaelli, O. Fagioli, P. Ceccarelli","doi":"10.1046/j.1439-0264.2001.00345.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00345.x","url":null,"abstract":"Histochemical staining techniques, which differentiate the main categories of carbohydrates, were applied to sections from different segments of the alimentary canal of the shi drum Umbrina cirrosa L. to study patterns of distribution of epithelial glycoconjugates. In the oesophagus, mucous cells contained sulphomucins, together with a small amount of sialomucins. Stomach epithelial cells secreted neutral and acidic glycoconjugates, while gastric glands only produced small quantities of sialomucins. Goblet cells showed the presence of sialo and sulphomucins in the pyloric caeca, whereas intestinal mucous cells secreted sulphated glycoconjugates. This work serves as a baseline for further studies on carbohydrate composition of the mucosa of the shi drum digestive system.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83463413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Histological and Histochemical Studies on the Lingual, Preglottal and Laryngeal Salivary Glands of the Japanese Quail (Coturnix coturnix japonica) at the Post‐hatching Period 日本鹌鹑(Coturnix Coturnix japonica)孵化后舌、声门前和喉唾液腺的组织学和组织化学研究
Anatomia Pub Date : 2001-12-01 DOI: 10.1046/j.1439-0264.2001.00353.x
N. Liman, G. Bayram, M. Kocak
{"title":"Histological and Histochemical Studies on the Lingual, Preglottal and Laryngeal Salivary Glands of the Japanese Quail (Coturnix coturnix japonica) at the Post‐hatching Period","authors":"N. Liman, G. Bayram, M. Kocak","doi":"10.1046/j.1439-0264.2001.00353.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00353.x","url":null,"abstract":"The postnatal development and histochemistry of mucins of the lingual, preglottal and laryngeal glands in the quails were investigated by means of light microscopy using specific staining for complex carbohydrates. In this study, the tongues were taken from female and male quails from day 1 to day 60 after hatching. The salivary glands in quail’s tongue comprised the lingual gland, with lateral and medial (paraentoglossal gland) portions that differ in morphology and histochemical staining, and the preglottal gland, with two lateral portions and one medial portion. The medial portion of the preglottal gland, which extended to the row of the laryngeal papillae on each side of the glottis, was described as the laryngeal gland. The salivary glands were present at hatching and their cells were functionally mature and secreted mucins. In quail of all ages, the histochemical reactions revealed that the cytoplasms of the secretory cells of the preglottal, laryngeal and paraentoglossal gland (medial portion of lingual gland) contained sialomucins and weakly sulphated epithelial mucins. Neutral mucins were absent in the paraentoglossal gland, while a small amount of neutral mucins was present in other glands. The mucins with vicinal diol groups, sialomucins and weakly sulphated epithelial mucins were mixed within the secretory cells of all the glands. All the histochemical reactions were restricted to the supranuclear regions of the secretory cells within the lateral portion of the lingual gland. In conclusion, the contents of mucins in the lingual, preglottal and laryngeal glands varied between different age groups, however, no differences in the glands’ histochemistry between male and female quails were observed.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82029745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 37
Immunocytochemical Analysis of Vimentin Expression Patterns in Porcine Embryos Suggests Mesodermal Differentiation from Day 9 after Conception 免疫细胞化学分析表明,猪胚胎中Vimentin表达模式在受孕后第9天开始向中胚层分化
Anatomia Pub Date : 2001-12-01 DOI: 10.1046/j.1439-0264.2001.00341.x
K. Prelle, W. Holtz, M. Osborn
{"title":"Immunocytochemical Analysis of Vimentin Expression Patterns in Porcine Embryos Suggests Mesodermal Differentiation from Day 9 after Conception","authors":"K. Prelle, W. Holtz, M. Osborn","doi":"10.1046/j.1439-0264.2001.00341.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00341.x","url":null,"abstract":"The expression of the intermediate filament proteins vimentin and keratin in porcine embryos was studied by whole‐mount immunocytochemistry between day 7 and day 11 after conception. Expression of vimentin was first detected in the inner cell mass of about 50% of the 9‐day‐old embryos. In elongated 11‐day‐old embryos, cells expressing vimentin were observed in the epiblast (after disappearance of Rauber’s membrane) and in cells migrating from the epiblast between the trophoblast and the underlying hypoblast layer. A keratin‐positive response was observed in trophectoderm cells at all stages. These findings suggest that inner cell mass cells in the pig start differentiating into mesodermal cells not later than day 9 after conception. While the delamination of the mesodermal germ layer is known to correlate with the loss of pluripotency of the inner cell mass cells, the early onset of mesodermal differentiation in the porcine embryo, characterized by vimentin expression and in contrast to the mouse, could in part be responsible for the lack of success in establishing pluripotent embryonic stem cell lines in this species. Our results suggest that further attempts to isolate inner cell mass‐derived pluripotent cells should be attempted well before day 9 after conception.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85580589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Localization and Coexistence of Calcium‐Binding Proteins and Neuropeptides in the Vagal Ganglia of the Goat 山羊迷走神经节钙结合蛋白和神经肽的定位与共存
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00333.x
T. Kang, H. S. Lee, I.‐S. Lee, C.-H. Lee
{"title":"Localization and Coexistence of Calcium‐Binding Proteins and Neuropeptides in the Vagal Ganglia of the Goat","authors":"T. Kang, H. S. Lee, I.‐S. Lee, C.-H. Lee","doi":"10.1046/j.1439-0264.2001.00333.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00333.x","url":null,"abstract":"This study was performed to investigate the neurochemical characteristics of the vagal ganglia of the goat by immunohistochemical methods using calbindin D‐28k (CB), calretinin (CR), parvalbumin (PA), substance P (SP), calcitonin gene‐related peptide (CGRP) and galanin (GAL) antibodies. In the proximal vagal ganglia (jugular ganglia), CGRP‐ (57.1%), SP‐ (48.2%), GAL‐ (8.6%), PA‐ (8.7%), CB‐ (8.5%) and CR‐like (5.3%) immunoreactive cells were observed. In the distal vagal ganglia (nodose ganglia), CGRP‐ (40.5%), SP‐ (30.2%), CB‐ (22.0%) and CR‐like (18.1%) immunoreactive cells were present. The double immunohistochemical study showed, that in the proximal vagal ganglia, CGRP immunoreactivity was co‐localized in SP‐ (84.8%), GAL‐ (100%), CB‐ (5.6%) and CR‐ (5.7%) immunoreactive cells; SP immunoreactivity was co‐localized in the CGRP‐ (80.0%), GAL‐ (100%), CB‐ (5.3%) and CR‐ (5.6%) immunoreactive cells; GAL immunoreactivity coexisted in the CGRP‐ (4.4%) and SP‐ (19.8%) immunoreactive cells, but not in calcium‐binding proteins (CBP)‐immunoreactive cells; PA immunoreactivity was absent in the CGRP‐ and SP‐immunoreactive cells; CB and CR immunoreactivities were seen in the CGRP‐ (0.8%) and SP‐immunoreactive (0.9%) cells. On the other hand, in the distal vagal ganglia, CGRP immunoreactivity appeared in SP‐ (66.6%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; SP immunoreactivities were observed in the CGRP‐ (44.1%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; CB immunoreactivities were present in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells; CR immunoreactivities were contained in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells. These findings indicate that the goat is distinct from other mammalian species in the distribution and localization of neurochemical substances in the vagal ganglia, and suggest that these differences may be related to physiological characteristics, particular those of the ruminant digestive system.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"139 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86449601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
The Anatomy of the Arterial Supply of the Thoracic Limb of the Porcupine (Hystrix cristata) 豪猪胸肢动脉供应的解剖学研究
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00332.x
S. Yılmaz, A. Aydın, Gürsel Dinç
{"title":"The Anatomy of the Arterial Supply of the Thoracic Limb of the Porcupine (Hystrix cristata)","authors":"S. Yılmaz, A. Aydın, Gürsel Dinç","doi":"10.1046/j.1439-0264.2001.00332.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00332.x","url":null,"abstract":"The aim of this study was to investigate the anatomy of the arterial supply and branches of the thoracic limb of the porcupine. With this aim, five (three male and two female) adult porcupines were used. The vascular tree of the thoracic limb was injected through the common carotid arteries with coloured latex. The a. thoracica externa arose from the a. axillaris at the level of the fourth rib. It divided into two branches and went to the m. cutaneus trunci and the m. pectoralis ascendens. The a. subscapularis came off the a. thoracodorsalis. Later, the a. circumflexa humeri caudalis et cranialis, the three muscular branches and the a. circumflexa scapulae arose from the a. subscapularis. The a. profunda brachii arose together with the a. bicipitalis from the same truncus. The a. collateralis ulnaris left from the a. brachialis independently. Rete carpi dorsale was formed by the a. radialis, the r. carpeus dorsalis of the a. collateralis ulnaris and the a. interossea caudalis. Arcus palmaris profundus was formed by the r. profundus of the a. interossea caudalis and the r. palmaris profundus of the a. radialis. In conclusion, despite a partial resemblance to that in the rat, the distribution of the thoracic limb arteries in the porcupine was found to be completely different from that of other mammals. These findings represent, to our knowledge, the first study on the distribution of thoracic limb arteries in the porcupine.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88058706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The Structure of the Masseter Muscle in the Giraffe (Giraffa camelopardalis) 长颈鹿咬肌的结构
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00342.x
M. Sasaki, H. Endo, H. Kogiku, N. Kitamura, J. Yamada, M. Yamamoto, K. Arishima, Y. Hayashi
{"title":"The Structure of the Masseter Muscle in the Giraffe (Giraffa camelopardalis)","authors":"M. Sasaki, H. Endo, H. Kogiku, N. Kitamura, J. Yamada, M. Yamamoto, K. Arishima, Y. Hayashi","doi":"10.1046/j.1439-0264.2001.00342.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00342.x","url":null,"abstract":"In the giraffe (Giraffa camelopardalis), the masseter muscle was divided into several layers. The superficial and more medial (second) tendinous sheets of the masseter muscle fused with each other at the dorso‐caudal part and a fleshy portion was located between these tendinous sheets. In the rostral part, the most superficial tendinous sheet turned around as a compact tendon and attached to the facial crest (Crista facialis). The turned tendinous sheet, however, never fused with the second tendinous sheet and this layer of the masseter muscle, that originated from the facial crest with the turned sheet, was inserted into the mandible with its fleshy portion. In the cattle, goat, sheep and Sika deer, the rostral layer of the masseter muscle arises from the facial crest with its fleshy portion and is inserted into the tubercle on the mandible through the strong tendinous sheet. In this study, the takin also showed the same structure of the masseter muscle. In the giraffe, however, the rostral layer inserted into the mandible through the strong tendinous sheet could not be distinguished, thus, there was no conspicuous tubercle on the mandible. Moreover in the masseteric region of the skull, the giraffe was similar to the Sika deer in several ways. However, it is suggested that the giraffe exerts smaller forces on the cheek teeth than does the Sika deer because of its longer Margo interalveolaris.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73619243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Topography, Structure and Function of the Patella and the Patelloid in Marsupials 有袋类动物髌骨和髌骨的地形、结构和功能
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00334.x
S. Reese, U. R. Pfuderer, H. Bragulla, K. Loeffler, K. Budras
{"title":"Topography, Structure and Function of the Patella and the Patelloid in Marsupials","authors":"S. Reese, U. R. Pfuderer, H. Bragulla, K. Loeffler, K. Budras","doi":"10.1046/j.1439-0264.2001.00334.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00334.x","url":null,"abstract":"The patella is a sesamoid bone that is found in most mammals and is regularly located in the insertion tendon of the quadriceps femoris muscle. Up to now, only a little has been known about the topography, structure and function of the patella in marsupials. Therefore the stifles of 61 marsupials of 30 different species were studied by radiography, necropsy and light microscopy. It was found that only the family of bandicoots (Peramelidae) possessed a typical patella. The other species revealed a patelloid consisting of fibrocartilage. The structure of the patelloid revealed common characteristic features in the following families of marsupials: (1) Dasyuridae, Phalangeridae, Pseudocheriidae, and Potoroidae; (2) Burramyidae; (3) Phascolarctidae, Vombatidae, and Dendrolagus; and (4) Superfamily Macropodoidea (except Dendrolagus and Potoroidae). Our results imply that the degree of differentiation in the fibrocartilage of the patelloid was strongly correlated to the mechanical forces acting on the patelloid which are a result of the specific kind of locomotion and the way of life. Generally the bony patella does not belong to the phylogenetic programme of the marsupials – with the exception of the bandicoots which are very closely related to the subclass of placental mammals (Eutheria).","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90409350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
The Application of Three‐Dimensional Internal Structure Microscopy in the Observation of Mare Ovary 三维内部结构显微镜在母马卵巢观察中的应用
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00335.x
Junpei Kimura, A. Tsukise, Hideo Yokota, Yasuo Nambo, T. Higuchi
{"title":"The Application of Three‐Dimensional Internal Structure Microscopy in the Observation of Mare Ovary","authors":"Junpei Kimura, A. Tsukise, Hideo Yokota, Yasuo Nambo, T. Higuchi","doi":"10.1046/j.1439-0264.2001.00335.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00335.x","url":null,"abstract":"The ovary of the mare has a unique structure which differs totally from that of other mammals. However, because of its relatively large size, conventional histological techniques were unsuitable for the observation of the internal structure of the whole ovary. Three‐dimensional internal structure microscopy (3D‐ISM) consists of a cryotome–CCD camera–laser disc recorder–PC‐based control system coupled with a graphic workstation. The internal structure of the ovary is observed by processing over more than 1000 stored images of serially sliced surfaces of each frozen equine ovary. The 3D reconstruction was done using the full‐coloured, volume‐rendering method. The relationship between the localization of medulla, cortex and ovulation fossa was clarified. The ovulation fossa is localized in the centre of the ovary and is surrounded by a broad ovarian cortex. A trace of ovulation was observed only at the ovulation fossa. Medulla are localized in narrow peripheral areas. The phenomenon of the competition to occupy the cortical area ahead of the ovulation fossa by developing secondary follicles was visualized. Spatial localization of various sized follicles was identified from 3D‐reconstructed images. In this study, it has been clarified that application of this novel computerized technique can clarify the anatomical arrangements of the equine ovary and the complex mechanism of equine follicular development.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80800114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Effects of Growth Hormone on Female Reproductive Organs 生长激素对女性生殖器官的影响
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00336.x
G. Kaiser, F. Sinowatz, G. Palma
{"title":"Effects of Growth Hormone on Female Reproductive Organs","authors":"G. Kaiser, F. Sinowatz, G. Palma","doi":"10.1046/j.1439-0264.2001.00336.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00336.x","url":null,"abstract":"During the last decade many experiments have been performed to study the effects of growth hormone (GH, somatotropin) on reproductive functions. Most of the studies found only slight or no effects of GH treatment, both on the oestrous cycle and on gonadotropin, progesterone, or oestrogen serum levels. In GH‐treated animals, elevated levels of insulin‐like growth factor I and GH in the serum could be correlated with an increased number of small (<5 mm in diameter) ovarian follicles, possibly as a consequence of a reduction of apoptosis and follicular atresia. There is still controversy over the effects of GH on in vivo and in vitro embryo production and on the gestation period. Recent studies produced some evidence that GH‐receptor is expressed in ovarian tissue, implying a direct role for GH in the ovary.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83476589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Ultrastructural Changes in Calvarial Osteoblast Cytoskeleton after Prostaglandin E2 Administration in Rats 前列腺素E2对大鼠颅骨成骨细胞骨架超微结构的影响
Anatomia Pub Date : 2001-10-01 DOI: 10.1046/j.1439-0264.2001.00340.x
K. Yamakura, H. Watanabe, S. Abe, T. Yanagisawa
{"title":"Ultrastructural Changes in Calvarial Osteoblast Cytoskeleton after Prostaglandin E2 Administration in Rats","authors":"K. Yamakura, H. Watanabe, S. Abe, T. Yanagisawa","doi":"10.1046/j.1439-0264.2001.00340.x","DOIUrl":"https://doi.org/10.1046/j.1439-0264.2001.00340.x","url":null,"abstract":"Recent studies on the cytoskeleton of osteoblasts have been made mainly using cultured cells. However, the morphology of cultured cells may be altered during subculture. Therefore, cytoskeletal changes of calvarial osteoblasts were investigated in situ by electron microscopy using the detergent perfusion method to preserve cell morphology as well as selectively observing the cytoskeleton in the presence of a high concentration of prostaglandin E2 (PGE2) in the calvarial periphery in rats. Rats were perfused with a mixture of Triton X‐100 and glutaraldehyde, then the cytoskeleton was observed by transmission electron microscopy. In osteoblasts of the control group, thick bundles of microfilaments ran parallel to the long axis of the cells immediately below the cell membrane adjacent to the osteoid layer. In contrast, in the osteoblasts of the PGE2‐administered group, the external morphology was changed to an asteroid or cubic shape, and thick bundles of microfilaments immediately below the cell membrane adjacent to the osteoid were not observed, although microfilament fibres, with a diameter of 5–6 nm, were observed in the cytoplasm.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73524078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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