{"title":"山羊迷走神经节钙结合蛋白和神经肽的定位与共存","authors":"T. Kang, H. S. Lee, I.‐S. Lee, C.-H. Lee","doi":"10.1046/j.1439-0264.2001.00333.x","DOIUrl":null,"url":null,"abstract":"This study was performed to investigate the neurochemical characteristics of the vagal ganglia of the goat by immunohistochemical methods using calbindin D‐28k (CB), calretinin (CR), parvalbumin (PA), substance P (SP), calcitonin gene‐related peptide (CGRP) and galanin (GAL) antibodies. In the proximal vagal ganglia (jugular ganglia), CGRP‐ (57.1%), SP‐ (48.2%), GAL‐ (8.6%), PA‐ (8.7%), CB‐ (8.5%) and CR‐like (5.3%) immunoreactive cells were observed. In the distal vagal ganglia (nodose ganglia), CGRP‐ (40.5%), SP‐ (30.2%), CB‐ (22.0%) and CR‐like (18.1%) immunoreactive cells were present. The double immunohistochemical study showed, that in the proximal vagal ganglia, CGRP immunoreactivity was co‐localized in SP‐ (84.8%), GAL‐ (100%), CB‐ (5.6%) and CR‐ (5.7%) immunoreactive cells; SP immunoreactivity was co‐localized in the CGRP‐ (80.0%), GAL‐ (100%), CB‐ (5.3%) and CR‐ (5.6%) immunoreactive cells; GAL immunoreactivity coexisted in the CGRP‐ (4.4%) and SP‐ (19.8%) immunoreactive cells, but not in calcium‐binding proteins (CBP)‐immunoreactive cells; PA immunoreactivity was absent in the CGRP‐ and SP‐immunoreactive cells; CB and CR immunoreactivities were seen in the CGRP‐ (0.8%) and SP‐immunoreactive (0.9%) cells. On the other hand, in the distal vagal ganglia, CGRP immunoreactivity appeared in SP‐ (66.6%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; SP immunoreactivities were observed in the CGRP‐ (44.1%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; CB immunoreactivities were present in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells; CR immunoreactivities were contained in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells. These findings indicate that the goat is distinct from other mammalian species in the distribution and localization of neurochemical substances in the vagal ganglia, and suggest that these differences may be related to physiological characteristics, particular those of the ruminant digestive system.","PeriodicalId":7888,"journal":{"name":"Anatomia","volume":"139 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"Localization and Coexistence of Calcium‐Binding Proteins and Neuropeptides in the Vagal Ganglia of the Goat\",\"authors\":\"T. Kang, H. S. Lee, I.‐S. Lee, C.-H. Lee\",\"doi\":\"10.1046/j.1439-0264.2001.00333.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study was performed to investigate the neurochemical characteristics of the vagal ganglia of the goat by immunohistochemical methods using calbindin D‐28k (CB), calretinin (CR), parvalbumin (PA), substance P (SP), calcitonin gene‐related peptide (CGRP) and galanin (GAL) antibodies. In the proximal vagal ganglia (jugular ganglia), CGRP‐ (57.1%), SP‐ (48.2%), GAL‐ (8.6%), PA‐ (8.7%), CB‐ (8.5%) and CR‐like (5.3%) immunoreactive cells were observed. In the distal vagal ganglia (nodose ganglia), CGRP‐ (40.5%), SP‐ (30.2%), CB‐ (22.0%) and CR‐like (18.1%) immunoreactive cells were present. The double immunohistochemical study showed, that in the proximal vagal ganglia, CGRP immunoreactivity was co‐localized in SP‐ (84.8%), GAL‐ (100%), CB‐ (5.6%) and CR‐ (5.7%) immunoreactive cells; SP immunoreactivity was co‐localized in the CGRP‐ (80.0%), GAL‐ (100%), CB‐ (5.3%) and CR‐ (5.6%) immunoreactive cells; GAL immunoreactivity coexisted in the CGRP‐ (4.4%) and SP‐ (19.8%) immunoreactive cells, but not in calcium‐binding proteins (CBP)‐immunoreactive cells; PA immunoreactivity was absent in the CGRP‐ and SP‐immunoreactive cells; CB and CR immunoreactivities were seen in the CGRP‐ (0.8%) and SP‐immunoreactive (0.9%) cells. On the other hand, in the distal vagal ganglia, CGRP immunoreactivity appeared in SP‐ (66.6%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; SP immunoreactivities were observed in the CGRP‐ (44.1%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; CB immunoreactivities were present in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells; CR immunoreactivities were contained in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells. These findings indicate that the goat is distinct from other mammalian species in the distribution and localization of neurochemical substances in the vagal ganglia, and suggest that these differences may be related to physiological characteristics, particular those of the ruminant digestive system.\",\"PeriodicalId\":7888,\"journal\":{\"name\":\"Anatomia\",\"volume\":\"139 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anatomia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1046/j.1439-0264.2001.00333.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anatomia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1046/j.1439-0264.2001.00333.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
摘要
本研究采用calbindin D‐28k (CB)、calretinin (CR)、parvalbumin (PA)、substance P (SP)、calcitonin gene - related peptide (CGRP)和galanin (GAL)抗体，采用免疫组织化学方法研究山羊迷走神经节的神经化学特征。在近端迷走神经节(颈神经节)中，观察到CGRP‐(57.1%)、SP‐(48.2%)、GAL‐(8.6%)、PA‐(8.7%)、CB‐(8.5%)和CR‐样(5.3%)免疫反应细胞。在远端迷走神经节(结节神经节)中，存在CGRP‐(40.5%)、SP‐(30.2%)、CB‐(22.0%)和CR‐样(18.1%)免疫反应细胞。双免疫组化研究显示，在迷走神经节近端，CGRP免疫反应性共定位于SP‐(84.8%)、GAL‐(100%)、CB‐(5.6%)和CR‐(5.7%)免疫反应细胞;SP免疫反应性共定位于CGRP‐(80.0%)、GAL‐(100%)、CB‐(5.3%)和CR‐(5.6%)免疫反应细胞;GAL免疫反应性在CGRP‐(4.4%)和SP‐(19.8%)免疫反应性细胞中共存，但在钙结合蛋白(CBP)免疫反应性细胞中不存在;CGRP -和SP -免疫反应细胞中不存在PA免疫反应性;CGRP‐(0.8%)和SP‐免疫反应(0.9%)细胞中可见CB和CR免疫反应。另一方面，在迷走神经节远端，CGRP免疫反应出现在SP‐(66.6%)、CB‐(1.0%)和CR‐(1.2%)免疫反应细胞中;在CGRP‐(44.1%)、CB‐(1.0%)和CR‐(1.2%)免疫反应细胞中观察到SP免疫反应;CGRP‐(0.5%)和SP‐(0.8%)免疫反应细胞中存在CB免疫反应;CGRP‐(0.5%)和SP‐(0.8%)免疫反应细胞中含有CR免疫反应。这些发现表明山羊在迷走神经节神经化学物质的分布和定位上与其他哺乳动物不同，并提示这些差异可能与生理特征有关，特别是反刍动物的消化系统。
Localization and Coexistence of Calcium‐Binding Proteins and Neuropeptides in the Vagal Ganglia of the Goat
This study was performed to investigate the neurochemical characteristics of the vagal ganglia of the goat by immunohistochemical methods using calbindin D‐28k (CB), calretinin (CR), parvalbumin (PA), substance P (SP), calcitonin gene‐related peptide (CGRP) and galanin (GAL) antibodies. In the proximal vagal ganglia (jugular ganglia), CGRP‐ (57.1%), SP‐ (48.2%), GAL‐ (8.6%), PA‐ (8.7%), CB‐ (8.5%) and CR‐like (5.3%) immunoreactive cells were observed. In the distal vagal ganglia (nodose ganglia), CGRP‐ (40.5%), SP‐ (30.2%), CB‐ (22.0%) and CR‐like (18.1%) immunoreactive cells were present. The double immunohistochemical study showed, that in the proximal vagal ganglia, CGRP immunoreactivity was co‐localized in SP‐ (84.8%), GAL‐ (100%), CB‐ (5.6%) and CR‐ (5.7%) immunoreactive cells; SP immunoreactivity was co‐localized in the CGRP‐ (80.0%), GAL‐ (100%), CB‐ (5.3%) and CR‐ (5.6%) immunoreactive cells; GAL immunoreactivity coexisted in the CGRP‐ (4.4%) and SP‐ (19.8%) immunoreactive cells, but not in calcium‐binding proteins (CBP)‐immunoreactive cells; PA immunoreactivity was absent in the CGRP‐ and SP‐immunoreactive cells; CB and CR immunoreactivities were seen in the CGRP‐ (0.8%) and SP‐immunoreactive (0.9%) cells. On the other hand, in the distal vagal ganglia, CGRP immunoreactivity appeared in SP‐ (66.6%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; SP immunoreactivities were observed in the CGRP‐ (44.1%), CB‐ (1.0%) and CR‐ (1.2%) immunoreactive cells; CB immunoreactivities were present in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells; CR immunoreactivities were contained in the CGRP‐ (0.5%) and SP‐ (0.8%) immunoreactive cells. These findings indicate that the goat is distinct from other mammalian species in the distribution and localization of neurochemical substances in the vagal ganglia, and suggest that these differences may be related to physiological characteristics, particular those of the ruminant digestive system.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
