Animal Feed Science and Technology最新文献

{"title":"Dietary carbohydrates induce a greater protein sparing effect than lipids in golden pomfret (Trachinotus ovatus) by modifying hepatic metabolic patterns","authors":"Yu-cai Guo ,&nbsp;Jian Zhong ,&nbsp;Ming-yan Huai ,&nbsp;Hai-qi Pu ,&nbsp;Bao-yang Chen ,&nbsp;An-qi Chen ,&nbsp;Wei Zhao ,&nbsp;Jin Niu","doi":"10.1016/j.anifeedsci.2025.116491","DOIUrl":"10.1016/j.anifeedsci.2025.116491","url":null,"abstract":"<div><div>In recent years, there has been a growing imperative to identify an economical alternative to protein, which is the primary energy source for aquatic commercial fish, owing to the cost of it has boomed continually. As is widely acknowledged, carbohydrate and lipid are two of the most cost-effective substitutes for protein in aquaculture due to their relatively low cost. However, which of them possess a better protein-sparing effect in low-protein diets for <em>Trachinotus ovatus</em> and the precise mechanism behind this difference remains elusive. An 8-week feeding trial was performed on 200 fish in floating cages for seawater to investigate whether carbohydrate or lipid offers a superior protein-sparing alternative in low-protein diets, alongside improved growth performance, antioxidant capacity, glycolipid metabolism, and protein synthesis in <em>T. ovatus</em>. The study encompassed one commercial feed diet (CF) and four low-protein diets: LP (a low protein diet with 36 % crude protein), HC (a high-carbohydrate diet containing 9 % cassava starch), HL (a high-lipid diet with 13 % crude lipid), and HCL (a high-carbohydrate-lipid diet incorporating 5 % cassava starch and 10.8 % crude lipid). The findings revealed that fish fed high carbohydrate diets exhibited significantly higher growth performance, feed utilization, and whole-body crude protein levels, along with a lower feed conversion ratio compared to those fed high lipid diets(HL and HCL groups). Furthermore, the findings pertaining to antioxidative enzyme activities and gene expression suggested that diets with high carbohydrate levels confer superior antioxidant capacity to the liver compared to diets low in protein. Conversely, diets high in lipid content may lead to excessive oxidative stress and inhibit catalase activity. More importantly, the HC group exhibited a substantial upregulation in the hepatic expression of <em>gk</em>, <em>pepck</em>, <em>srebp1</em> and <em>hsl</em>, suggesting an activation of hepatic glucose and lipid catabolism. Conversely, the expression of <em>g-6-p</em>, <em>igf</em>, <em>glp-1</em>, <em>fas</em>, <em>acc</em>, agpat<em>3</em>, <em>elovel5</em>, <em>pparα</em>, <em>lpl</em> and <em>cpt1</em> were markedly downregulated, implying a possible attenuation in anabolic processes. This pattern indicates that hepatic glucose and lipid metabolism may be skewed towards catabolism and away from anabolism in the HC group. Besides, the expression patterns of protein synthesis in muscle tissue suggest that a high carbohydrate diet inhibits both the PI3K-AKT and mTOR-S6K signaling pathways, potentially leading to the suppression of muscle growth. In conclusion, a suitable addition of carbohydrate in the diet of <em>T. ovatus</em> elicits a more pronounced protein-sparing effect compared to lipids, achieved through the modulation of hepatic metabolic patterns.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116491"},"PeriodicalIF":2.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical composition of feed ingredients influences equine gastric ecosystem activity in vitro","authors":"Samy Julliand ,&nbsp;Véronique Julliand ,&nbsp;Pauline Grimm","doi":"10.1016/j.anifeedsci.2025.116494","DOIUrl":"10.1016/j.anifeedsci.2025.116494","url":null,"abstract":"<div><div>When the pH of the gastric environment decreases below 4, acidity and volatile fatty acids (VFAs) can damage the epithelium. Depending on the feed ingredients incorporated in horse feeds, gastric ecosystem can be modified. Dehydrated alfalfa might be of interest because of its biochemical composition, which provides a strong acid buffering capacity. We ran an <em>in vitro</em> trial to assess the impact of three dehydrated alfalfas differing in biochemical composition on the persistence of acid buffering capacity over time in a gastric ecosystem undergoing fermentation. Barley and wheat bran were used for comparison.</div><div>Five substrates were prepared from 0.5 g dry matter (DM) barley, completed with 0.5 g DM alfalfa, wheat bran, or barley. Substrates were added to a modified Lowe culture medium in bottles under sterile conditions. After inoculation with gastric contents collected from horses, the bottles were incubated anaerobically for 10-hours. Every two hours, the gas produced, the concentration of VFAs and lactate, the pH, and the amount of 0.1 M hydrochloric acid (HCl) required to decrease the pH of the solution down to 4 were measured in triplicate.</div><div>The pH of the feed ingredients was close (5.81 −6.29), but 7 −12 times more HCl was needed to bring the pH down to 4 with alfalfa compared to barley, and 3 −5 times more compared to wheat bran. This acid buffering capacity was correlated with ash content (r = 0.984; P = 0.002), and particularly calcium content (r = 0.995; P &lt; 0.001). Fermentations started faster in bottles containing alfalfa (P &lt; 0.001 after 2 h), but then gas production was higher with barley and wheat bran (P &lt; 0.001). However, VFA concentrations remained higher with alfalfa than other substrates (P &lt; 0.05), and the higher the content in proteins and simple sugars the more pronounced the differences. While the pH of the solution and the amount of HCl required to decrease to pH4 fell sharply over the 10-hour monitoring period, these variables remained higher with alfalfa compared to barley and wheat bran substrates (P &lt; 0.001 after 6-hours for pH; P &lt; 0.05 after 4-hours for HCl amount). Despite their different biochemical composition, the impact of the three alfalfas in the environment was close. These results suggest that adding alfalfa to feeds could contribute maintaining a higher pH in the horse stomach. This seems to arise from intrinsic high acid buffering capacity of alfalfa, rather than from the reduction in gastric fermentations. Considering the acid buffering capacity of feed ingredients in the formulation of horse feed could help maintain a physiological gastric pH for longer and reduce gastric diseases.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116494"},"PeriodicalIF":2.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of two shrimp head meals as dietary ingredients for Nile tilapia Oreochromis niloticus: Effects on nutrient digestibility, amino acid chemical score, growth performance, digestive enzyme activity and antioxidant parameters","authors":"Guilherme Melgaço Heluy ,&nbsp;Maria Angélica da Silva ,&nbsp;Vivian Costa Vasconcelos ,&nbsp;José Domingos Carneiro de Arcanjo ,&nbsp;Douglas Lemos de Souza ,&nbsp;Marcele Trajano de Araújo ,&nbsp;Thiago Barbosa Cahú ,&nbsp;Bruno Oliveira de Veras ,&nbsp;Ronaldo Olivera Cavalli ,&nbsp;Maria do Carmo Mohaupt Marques Ludke ,&nbsp;Juliana Ferreira dos Santos ,&nbsp;Ranilson de Souza Bezerra","doi":"10.1016/j.anifeedsci.2025.116492","DOIUrl":"10.1016/j.anifeedsci.2025.116492","url":null,"abstract":"<div><div>The digestibility and enzymatic and antioxidant effects of shrimp head meal (<em>Penaeus vannamei</em>) as a protein source in the diet of Nile tilapia (<em>Oreochromis niloticus</em>) were evaluated. Two shrimp meals were tested: whole shrimp meal (WSM) and low-lipid shrimp meal (LLSM). Dietary levels of 20 % and 30 % of shrimp meal replaced 80 % and 70 % of the reference diet, respectively. The 60-day experiment was conducted in a modified Guelph system, with nine feeding tanks and nine fecal collection units, each containing 10 fish (61.85 ± 10.06 g). WSM and LLSM supported high digestibility of dry matter, protein, energy, and minerals, with values often exceeding 90 % at 20 % inclusion, except for chitin. Growth performance indicators (weight gain, feed conversion ratio, protein efficiency ratio, and survival) were consistently improved in shrimp-meal diets at both inclusion levels compared with the reference diet, confirming the biological relevance of digestibility results. Essential and non-essential amino acids were highly digestible, with no limiting amino acids, and the chemical score and indispensable amino acid index of both WSM and LLSM exceeded those of protein sources conventionally used in aquafeeds. Inclusion of 20 % and 30 % significantly increased digestive enzyme activities (alkaline protease, trypsin, chymotrypsin, lipase, and amylase), while hepatic antioxidant capacity was enhanced through catalase and reduced glutathione activities, without changes in malondialdehyde levels. Overall, shrimp head meals represent high-quality, bioavailable protein sources for Nile tilapia, contributing simultaneously to improved growth performance and to circular economy strategies through the valorization of shrimp industry by-products.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116492"},"PeriodicalIF":2.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spray-dried plasma titration effects on growth performance, intestinal morphology, and immune system indicators of weaned pigs housed in a sanitation-challenged environment","authors":"Hannah M. Bailey ,&nbsp;Natalia S. Fanelli ,&nbsp;Joy M. Campbell ,&nbsp;Hans H. Stein","doi":"10.1016/j.anifeedsci.2025.116484","DOIUrl":"10.1016/j.anifeedsci.2025.116484","url":null,"abstract":"<div><div>An experiment was conducted to test the hypothesis that increasing inclusion of spray-dried plasma (SDP) in diets improves growth performance and intestinal morphology and reduces inflammation in newly weaned pigs. Four-hundred weaned pigs (body weight: 6.05 ± 0.80 kg) were allotted to a randomized complete block design with 5 diets and 2 blocks (16 pens/diet; 5 pigs/pen). Pigs were placed in pens that were not cleaned to create a sanitation challenge. Phase 1 diets containing 0, 20, 40, 60, or 80 g/kg SDP were formulated. One pig per pen was sacrificed on d 14 and samples of intestinal tissue and mucosa were collected. During phase 1, growth performance parameters and body weight of pigs on day 14 increased (linear, P &lt; 0.05) with increasing dietary SDP. Villus width in the jejunum of pigs on day 14 tended to increase (linear, P &lt; 0.10) with increasing inclusion of SDP, and villus height to crypt depth ratio tended to be the greatest (quadratic, P &lt; 0.10) for pigs fed a diet with 80 g/kg SDP. The jejunal mucosa concentration of interleukin- (IL-) 2 tended to be least (quadratic, P &lt; 0.10) at 80 g/kg inclusion of SDP and IL-8 tended to increase (linear, P &lt; 0.10) as SDP inclusion increased in the diet. Secretory immunoglobulin A in the ileal mucosa was greatest at 20 g/kg inclusion of SDP to the diet and then decreased with increasing dietary SDP (quadratic, P &lt; 0.05), and IL-10 tended to be least at 40 and 60 g/kg dietary SDP, but increased with 80 g/kg dietary SDP (quadratic, P &lt; 0.10). Activated T cells and the ratio of activated to regulatory T cells tended to be greatest at 40 g/kg dietary SDP but then decreased as SDP increased in the diet (quadratic, P &lt; 0.10), whereas systemic lymphocytes linearly decreased (P &lt; 0.05) as SDP increased in the diet. The concentration of plasma urea N also linearly decreased (P &lt; 0.05) as dietary SDP increased, indicating greater amino acid utilization with greater dietary SDP. In conclusion, the optimal inclusion of SDP in diets for weanling pigs was at least 80 g/kg as indicated by improvements in growth performance and utilization of amino acids, but data for intestinal morphology and mucosal and systemic inflammation did not result in a conclusive optimum concentration of dietary SDP.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116484"},"PeriodicalIF":2.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation quality, chemical composition, and antioxidant capacity of alfalfa and corn silages inoculated with exopolysaccharide-producing lactic acid bacteria","authors":"Zohreh Akhavan Kharazian ,&nbsp;Mengya Jia ,&nbsp;Rina Su ,&nbsp;Farhad Ahmadi ,&nbsp;Xusheng Guo","doi":"10.1016/j.anifeedsci.2025.116490","DOIUrl":"10.1016/j.anifeedsci.2025.116490","url":null,"abstract":"<div><div>This study evaluated the effects of exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) on fermentation quality, chemical composition, and antioxidant capacity of alfalfa and corn silage. <em>Pediococcus pentosaceus</em> L73, <em>Pediococcus acidilactici</em> L74, and <em>Lactiplantibacillus plantarum</em> L75 strains were used as silage inoculants to assess their ability to enhance fermentation quality and antioxidant activity. Compared to non-inoculated silages, the LAB inoculation increased lactic acid and (in most treatments) acetic acid concentrations in both alfalfa and corn silage. LAB inoculation had no effect on the pH of corn silage but in alfalfa silage, it decreased the pH from 5.2 to 4.4. This may reflect a more efficient fermentation process compared to non-inoculated silages. Inoculated silages had a lower water-soluble carbohydrate content, an indication of their consumption by LAB. Corn silage inoculated with EPS-producing LAB strains had higher T-AOC in several treatments and elevated SOD activity in selected strains, though glutathione peroxidase (GSH-Px) activity was undetectable. Alfalfa silage treated with the LAB strains demonstrated increased GSH-Px activity in L75 and L73 + L75, with SOD and T-AOC increases observed in selected treatments. These findings suggest that EPS-producing LAB strains may improve both fermentation quality and antioxidant activity of silages, with alfalfa silage demonstrating greater benefits in antioxidant capacity compared to corn silage. These improvements may contribute to better animal health and enhance the nutritional value of animal-derived products.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116490"},"PeriodicalIF":2.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding a clay mineral-based feed additive improves performance, eating behavior and liver health by feedlot beef cattle fed a high-concentrate diet","authors":"D.I.C.G. Gouvêa ,&nbsp;M.B. Niehues ,&nbsp;V.V. Carvalho ,&nbsp;A. Perdigão ,&nbsp;J. Faas ,&nbsp;I. Artavia ,&nbsp;M.C.S. Pereira ,&nbsp;M.D.B. Arrigoni ,&nbsp;C.L. Martins","doi":"10.1016/j.anifeedsci.2025.116489","DOIUrl":"10.1016/j.anifeedsci.2025.116489","url":null,"abstract":"<div><div>Feedlot cattle are continuously exposed to liver health challenges during the finishing period, either due to high-energy diet intake or mycotoxin presence, which can cause liver damage, impairing hepatic metabolism and overall performance. This study evaluated the impacts of adding clay mineral-based feed additive with plant extracts (CMFA) on performance, eating behavior, carcass traits, blood metabolites, and liver health of feedlot Nellore cattle. The trial was a completely randomized design, animal was considered an experimental unit. Forty-eight 22-month-old Nellore bulls (402.50 kg ± 4.95 kg) were fed in a collective pen, containing six electronic bunks with automatic selective doors for individual feeding and feed intake monitoring according to two treatments: Control (without CMFA) and CMFA (1.5 g/kg of dry matter [DM]). The study lasted 96 days. CMFA-fed animals had greater final body weight (<em>P</em> = 0.04), average daily gain (<em>P</em> = 0.05), final hot carcass weight (HCW; <em>P</em> = 0.05), and HCW daily gain (<em>P</em> = 0.05) compared to the control group. In addition, feeding CMFA altered the feeding behavior, reducing total feeding time and the number of meals, whereas increasing the DM intake rate. CMFA-fed cattle demonstrated lower aspartate aminotransferase serum concentrations and reduced histopathological scores of inflammations and liver injury, including inflammatory infiltrate, hepatitis, focal necrosis, bile stasis, and bile duct hyperplasia, demonstrating better hepatic tissue integrity. Thus, adding a clay mineral-based feed additive to a high-concentrate diets can improve performance by mitigating inflammation and improving liver health of feedlot Nellore cattle.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116489"},"PeriodicalIF":2.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary lipid sources on feed palatability, growth, fecal characteristics, blood parameters and antioxidant activity in British shorthair kittens (Felis catus)","authors":"Jiahuan Wang ,&nbsp;Jia Li ,&nbsp;Wencong Li ,&nbsp;Nengbin Zhu ,&nbsp;Hongsen Xu ,&nbsp;Lihe Liu ,&nbsp;Yubo Chen","doi":"10.1016/j.anifeedsci.2025.116488","DOIUrl":"10.1016/j.anifeedsci.2025.116488","url":null,"abstract":"<div><div>This study aims to investigate the effects of various dietary lipid sources on feed palatability, growth performance, fecal characteristics, blood parameters and antioxidant activity in British shorthair kittens. British shorthair kittens (n = 36; average weight, 2.05 ± 0.06 kg) were fed diets supplemented with chicken fat (CF), flaxseed oil (FO), beef fat (BT), and sunflower oil (SO) in a preliminary experiment to assess feed palatability. Among the diets, CF and BT diets had higher intakes and preference. Thereafter, 20 kittens (1.39 ± 0.05 kg) were fed the diets for 8 weeks to assess growth performance. Body weight gain within 28 days was significantly lower in SO group than in CF group (<em>P</em> &lt; 0.05). Additionally, the incidences of diarrhea, soft stool, and very firm/dry stool were highest in SO, FO, and BT groups, respectively. Moreover, total antioxidant capacity (T-AOC) was higher in CF and BT groups than in the other groups. Among the groups, FO group exhibited the highest catalase (CAT) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) levels. Furthermore, SO group exhibited the lowest levels blood total protein and albumin levels and the highest bile acid and total cholesterol levels. Notably, only aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were within the reference range in CF group. Conclusively, CF and BT supplementation positively influences palatability and growth performance, with CF showing additional benefits for digestive health and liver function. Moreover, oxidative stress was higher in FO and SO groups than in the CF and BT groups.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116488"},"PeriodicalIF":2.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary cannabidiol and nanoselenium mediates post-infection changes in the profiles of fatty acids, sarcoplasmic proteins, and meat properties of C. perfringens-challenged chickens","authors":"M. Kinsner ,&nbsp;D. Szkopek ,&nbsp;D. Jaworska ,&nbsp;A. Kotlarska ,&nbsp;W. Przybylski ,&nbsp;D. Bień ,&nbsp;M. Michalczuk ,&nbsp;A. Cieślak ,&nbsp;K. Kozłowski ,&nbsp;P. Matusevičius ,&nbsp;P. Konieczka","doi":"10.1016/j.anifeedsci.2025.116487","DOIUrl":"10.1016/j.anifeedsci.2025.116487","url":null,"abstract":"<div><div>Dietary Cannabidiol (CBD) and Se-nanoparticles (SeNPs) have shown promising results in supporting health status in chickens but their action and optimal inclusion levels is unknown. A total of 432 one-day-old male Ross 308 broilers were reared in cages for 35 days. The negative control (CON) received a basal diet without supplementation or challenge, while the positive control (CON+) was <em>Clostridium perfringens</em>-challenged. The CBD+ group was <em>C. perfringens-</em>challenged and supplemented with 15<!--> <!-->g/kg <em>Cannabis</em> extract, while the SeNPs+ was challenged but received 0.3<!--> <!-->mg/kg diet SeNPs. Birds from CBD + SeNPs and CBD + SeNPs + <em>C. perfringens</em> were fed both additives, with the latter group also <em>C. perfringens</em>-challenged. Supplementation with CBD and/or SeNPs did not compromised bird’s performance, breast meat quality or the desaturation, thrombogenic and atherogenicity indices. CBD and CBD + SeNPs significantly increased polyunsaturated fatty acid (PUFA) and omega-3 PUFA concentrations of <em>C. perfringens</em>-challenged chickens. PUFA n-6/n-3 ratios in muscle lipids were most significantly associated with SeNPs alone or in combination with CBD (P&lt;0.05). The levels of meat exudate pyruvate kinase/phosphoglucose isomerase from the CBD + SeNPs were significantly different from CON, CON+ and CBD+ groups. During the experimental period, the birds consumed 0.39<!--> <!-->g of tetrahydrocannabinol and 9.41<!--> <!-->g of CBD resulting in THC accumulation in breast muscle at a concentration of 10 ppm and CBD transfer at 79 × 10^-6. These findings provide new understanding of how CBD and SeNPs influence the nutrition-challenge-meat quality relationship, highlighting the need for additional research to fully elucidate their effects.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116487"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct and carry-over effects of supplementation on growth performance, metabolic profile, and intramuscular adipogenesis of grazing beef heifers","authors":"Letícia Zamberlan Pistillo ,&nbsp;Pedro Henrique Borba Pereira ,&nbsp;Renato Duarte de Araujo ,&nbsp;Pedro Veiga Rodrigues Paulino ,&nbsp;Márcio de Souza Duarte ,&nbsp;Edenio Detmann ,&nbsp;Cláudia Batista Sampaio","doi":"10.1016/j.anifeedsci.2025.116486","DOIUrl":"10.1016/j.anifeedsci.2025.116486","url":null,"abstract":"<div><div>This study evaluated the direct and carry-over effects of nutritional strategies during the suckling and backgrounding phases on performance, blood metabolites, and gene expression related to lipid metabolism in grazing F1 Red Angus × Nellore heifers. Thirty-four female calves were assigned in a 2 × 2 factorial design: with or without creep feeding during the suckling phase and low or high level of supplementation during backgrounding. All animals were finished on intensive grazing in a common nutritional treatment. Performance traits, blood parameters, muscle ether extract content, and the expression of adipogenic and lipogenic genes (ZFP423, PPARγ, ACC, CPT1β, PGC1α) were assessed. Creep feeding increased weight (<em>P = 0.073</em>) and average daily gain (ADG) (<em>P = 0.079</em>) at weaning, and altered lipid and nitrogen metabolism markers, including reductions in cholesterol (<em>P = 0.007</em>), HDL (<em>P = 0.004</em>), and creatinine (<em>P = 0.03</em>), and increase the serum urea nitrogen (<em>P &lt; 0.10</em>). During backgrounding, high level supplementation improved body weight (<em>P = 0.04</em>), ADG (<em>P = 0.02</em>), and increased concentrations of glucose (<em>P = 0.07</em>) cholesterol (<em>P = 0.01</em>), triglycerides (<em>P = 0.001</em>), albumin (<em>P = 0.01</em>), and IGF1 (<em>P = 0.01</em>). No interactive effects between phases were observed (<em>P &gt; 0.10</em>). In the finishing phase, slaughter body weight was affected (<em>P = 0.04</em>) being higher in animals previously fed high level. No effects of supplementation strategy were detected on carcass traits, muscle ether extract, or gene expression. These results indicate that early-life supplementation improves intermediate performance and metabolic status, but does not alter carcass composition or intramuscular fat gene markers at slaughter.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116486"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feed additive carriers affected the physiological effects of a lactobacilli-based pro- and postbiotic in broilers","authors":"Samuel C.G. Jansseune ,&nbsp;Wouter H. Hendriks ,&nbsp;Marie-Hélène Pinard van der Laan ,&nbsp;Fany Blanc","doi":"10.1016/j.anifeedsci.2025.116485","DOIUrl":"10.1016/j.anifeedsci.2025.116485","url":null,"abstract":"<div><div>To promote growth performance and health of broiler during production, additives, like pro- and postbiotics, are often used and most of them use carriers. This study aimed to investigate if carriers can modulate the physiological effects of two lactobacilli-based pro- (<strong>Pro</strong>) and postbiotic (<strong>Post</strong>) additives, and to gain more insight into their physiological effects in the absence of carriers, in broilers fed a non-starch polysaccharide-rich diet reared under practical housing conditions. A total of 1,600, one-day-old male Ross 308 chicks were equally distributed among 5 treatments (control, Pro and Post with and without their respective carriers) with 8 replicate pens each and 40 broilers/pen. Growth performance parameters, footpad lesions, and blood plasma physiological parameters related to nutrition and immunity were determined. On d35, Pro increased body weight (<strong>BW</strong>) (+2.1%; p = 0.025), while its carrier negated this effect (-1.9%; p = 0.047). Post did not affect d35 BW but increased the feed conversion ratio (<strong>FCR</strong>) by 1.3% (p = 0.041), while its carrier increased BW (+2.4%; p = 0.013), and lowered FCR (-1.3%; p = 0.042). Growth performance parameters showed a beneficial effect of Pro but not Post, while the use of their respective commercial carriers had a growth-depressing and -promoting effect, respectively. Carriers also affected the footpad lesion score, and blood parameters related to nutrition and immunity. These results highlight that additive carriers can modify the effects on broiler growth and metabolism of the pro- and postbiotic additives to which they are added. The occurrence of an additive-carrier interaction indicates that carriers for pro- and postbiotic additives for broilers must be carefully selected.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"329 ","pages":"Article 116485"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}