Animal Feed Science and Technology最新文献

{"title":"Improving lipid utilization and growth through lecithin inclusion in diets for giant grouper (Epinephelus lanceolatus)","authors":"Grace Butler ,&nbsp;Caroline Lourdes Candebat ,&nbsp;Simon Kumar Das ,&nbsp;Leo Nankervis","doi":"10.1016/j.anifeedsci.2025.116393","DOIUrl":"10.1016/j.anifeedsci.2025.116393","url":null,"abstract":"<div><div>Giant grouper (<em>Epinephelus lanceolatus</em>) is a culturally and economically significant farmed fish species in Asia and an emerging aquaculture species in Australia. However, the historical reliance on ‘trash fish’ as feed has hindered the development of species-specific formulated diets. Preliminary findings suggest that methionine and choline influence lipid metabolism, highlighting the need to re-evaluate dietary lipid levels for optimal growth. Building on these findings, this study examined how dietary lipid levels and phospholipid (lecithin) inclusion interact to influence lipid metabolism and growth performance in <em>E</em>. <em>lanceolatus</em>. Six isoproteic diets (44.47 ± 0.05 % protein) were formulated with 10 % or 15 % lipid and 0, 0.5 %, or 1 % lecithin inclusion. The results showed that increasing lipid content from 10 % to 15 %, combined with 1 % lecithin inclusion, enhanced weight gain by 12.14 %. This growth-promoting effect was associated with elevated circulating triglycerides and altered cholesterol and glucose levels, suggesting improved lipid transport and utilization. In contract, no growth improvement was observed with 0 % or 0.5 % lecithin inclusion. These findings demonstrate the critical role of lecithin in optimizing lipid metabolism and growth performance in juvenile <em>E</em>. <em>lanceolatus</em>, providing a foundation for sustainable, species-specific diet formulations.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116393"},"PeriodicalIF":2.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A phytogenic feed additive blend as a replacement for monensin in lactating Rahmani ewes' diets: Effects on feed utilization and milk production","authors":"Hossam H. Azzaz ,&nbsp;Noha A. Hassaan ,&nbsp;Gouda A. Gouda ,&nbsp;Yanting Chen ,&nbsp;Ahmed E. Kholif","doi":"10.1016/j.anifeedsci.2025.116397","DOIUrl":"10.1016/j.anifeedsci.2025.116397","url":null,"abstract":"<div><div>This study evaluated the potential of phytogenic feed additives (PFAs) as a replacement for monensin in the diets of lactating Rahmani ewes. Two experiments were conducted using a completely randomized design. The first was an <em>in vitro</em> study that evaluated the effects of increasing doses (0, 0.3, 0.6, and 1 g/kg dry matter [DM]) of PFAs and monensin. The second experiment was an <em>in vivo</em> trial involving 30 lactating ewes (53 ± 0.5 kg body weight), all in their first week of lactation (1–7 days in milk). The ewes were randomly assigned to one of three dietary treatments: control, 0.3 g PFAs, or 0.3 g monensin/ kg DM diet, with 10 ewes per treatment, over a 60-day period. The doses for PFAs and monensin were selected based on the optimal results observed in the <em>in vitro</em> experiment. Before the 60-day experimental period, all ewes underwent a 14-day adaptation period to their respective diets. In the <em>in vitro</em> study, both PFAs and monensin significantly reduced gas and methane production (<em>p</em> &lt; 0.01), with the most substantial reductions observed at the 1 % inclusion level, decreasing by up to 17.6 % and 35.3 %, respectively. Digestibility of DM, organic matter (OM), and fiber improved at moderate inclusion levels (<em>p</em> &lt; 0.01) but declined at 1 %. Additionally, short-chain fatty acids were significantly reduced (<em>p</em> &lt; 0.01) with both additives. In the lactation experiment, supplementation improved nutrient digestibility, including DM, OM, crude protein, and fiber (<em>p</em> &lt; 0.01), with no differences between PFAs and monensin. Blood parameters such as total protein, albumin, glucose, and cholesterol were positively influenced (<em>p</em> &lt; 0.05), while blood urea nitrogen and liver enzymes decreased (<em>p</em> &lt; 0.01). Milk yield, energy output, and feed efficiency improved significantly (<em>p</em> &lt; 0.001), with no differences between the two supplements. The milk fatty acid profile showed reductions in total saturated fatty acids (SFA) and increases in unsaturated fatty acids (UFA), with a higher UFA:SFA ratio. Specific fatty acids, such as α-linolenic acid, increased significantly (<em>p</em> = 0.019). This study concludes that PFAs, at 0.3 g/kg, could effectively replace monensin to improve feed efficiency and lactational performance in Rahmani ewes.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116397"},"PeriodicalIF":2.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of phytobiotics in the form of complexed organometallic phytoncides and micronized herbs in the nutrition of Ross 308 broiler chickens: Effects on growth performance, meat quality, and immune response","authors":"Marcin Gumowski ,&nbsp;Cassandra Ceccopieri ,&nbsp;Jan P. Madej ,&nbsp;Katarzyna Leicht ,&nbsp;Małgorzata Korzeniowska ,&nbsp;Anna Lipińska ,&nbsp;Kamil Sierżant ,&nbsp;Damian Konkol ,&nbsp;Henryk Różański ,&nbsp;Muhammad Umair Asghar ,&nbsp;Mariusz Korczyński","doi":"10.1016/j.anifeedsci.2025.116391","DOIUrl":"10.1016/j.anifeedsci.2025.116391","url":null,"abstract":"<div><div>Phytobiotics are being investigated as possible substitutes for synthetic growth promoters in poultry feeding because to the increased demand in natural feed additives. This investigation assesses the impact of several phytobiotic formulations on the morphology of the intestinal and lymphoid organs, meat quality, and broiler performance. A total of 1280 Ross 308 broiler chickens were allocated into five experimental groups, including two control groups and three groups administered varying kinds or doses of phytobiotic prototypes. During the course of the 35-day study, key performance metrics such as body weight, feed conversion ratio, and mortality were observed. In addition to histological examinations of the intestines and lymphoid organs to evaluate morphological changes, measurements of pH, colour, tenderness, and chemical composition were used to evaluate the quality of the meat. The findings demonstrated that a single herbal prototype increased (P ≤ 0.05) the body weight of broilers at seven and thirty-five days, with effects similar to those of antibiotic growth promoters. Additionally, this group’s leg muscles showed less drip loss (P ≤ 0.05), which suggests that their meat quality was improved and their ability to retain water was improved. In comparison to controls, chickens that were fed lower concentrations of the same prototype exhibited significantly wider ileal villi (P ≤ 0.05), whereas a second prototype resulted in an increase in villus size. The investigation further demonstrated a rise in CD8 + cells in the spleens of broilers given certain phytobiotic formulations (P ≤ 0.05), suggesting a possible improvement in the immune response via the Th1 signalling pathway. In summary, phytobiotic preparations provide a viable substitute for traditional growth-promoting chemicals in poultry production, with the potential to improve broiler performance, meat quality, and gut health. These results are a valuable addition to the expanding body of evidence that underpins the utilization of plant-derived substances in poultry nutrition.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116391"},"PeriodicalIF":2.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emulsifier is beneficial for declining dietary lipid requirement of aquatic crustaceans by accelerating the absorption, transport and deposition of lipids: A case study in mud crab (Scylla paramamosain)","authors":"Zihao Ye ,&nbsp;Jinjin Liu ,&nbsp;Shihui Cui ,&nbsp;Jing Chen ,&nbsp;Mengyao Tang ,&nbsp;Chaojia Chen ,&nbsp;Yifang Xu ,&nbsp;Ziyi Wang ,&nbsp;Yuan Huang ,&nbsp;Shaowei Zhai ,&nbsp;Ziping Zhang ,&nbsp;Chao Fan ,&nbsp;Xuexi Wang","doi":"10.1016/j.anifeedsci.2025.116389","DOIUrl":"10.1016/j.anifeedsci.2025.116389","url":null,"abstract":"<div><div>To investigate the potential of emulsifiers in reducing dietary lipid requirements of juvenile <em>Scylla paramamosain</em> and the associated physiological and biochemical responses, three isonitrogenous experimental diets (45 % crude protein content) with 10 % lipid content (FO, fish oil as the main lipid source), 5 % lipid content (LF) and 5 % lipid content with the addition of 0.2 % emulsifier (LF+EMU) were formulated. A total of 30 juveniles mud crabs (30.56 ± 0.56 g) per treatment was cultured for 12 weeks in single crab cells. Compared to LF, the LF+EMU group had higher weight gain and specific growth rate. Crabs in the LF+EMU group had significantly lower lipid contents in muscle and hepatopancreas, along with reduced lipase activity, resorptive and blister-like cell quantity in hepatopancreas than those in FO group, while these values were higher than that in crabs from LF group. Emulsifier supplementation into LF diet elevated the activity of superoxide dismutase, glutathione peroxidase and catalase, while exhibiting a significantly decrease in malondialdehyde content in hepatopancreas. Furthermore, the addition of an emulsifier into LF diet did not only upregulate genes associated with lipid anabolism like <em>sterol regulatory element binding protein 1</em>, <em>glucose-6-phosphate dehydrogenase, delta-9 fatty acyl desaturase and fatty acid synthase,</em> but also upregulate genes related to lipid catabolism such as <em>acyl-CoA oxidase 3</em>, <em>carnitine palmitoyl transferase Ⅰ</em>, and transport such as <em>fatty acid-binding protein 1 and 3</em>, <em>fatty acid transport protein 4, scavenger receptor b, low-density lipoprotein receptor</em> and <em>low-density lipoprotein receptor-related protein 2</em>. The analysis of intestinal microbiota revealed that crabs fed with LF+EMU and FO had significantly higher Simpson, Shannon, Ace, and Chao 1 than those fed with LF diet. In the LF+EMU treatment, the abundances of Fusobacteria and Bacteroidetes phyla, along with the genera <em>Photobacterium</em> and <em>Hypnobacterium</em>, were significantly elevated compared to LF treatment, the abundances of <em>Candidatus_Hepatoplasma</em> genera presented an opposite trend. Therefore, emulsifier supplementation in low-fat diet could regulate intestinal microbiota, accelerate secretion and activities of digestive enzymes, facilitate the upregulation of genes associated with lipid metabolism, and thus improve the absorption, transport, deposition of lipid and growth performance and indicate the potential in diminishing dietary lipid requirement of <em>S. paramamosain</em>.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116389"},"PeriodicalIF":2.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal myo-inositol requirement for growth performance in juvenile freshwater crayfish","authors":"Changchang Pu ,&nbsp;Yuanyi Liu ,&nbsp;Ruyi Sun ,&nbsp;Zihui Wei ,&nbsp;Bingke Wang ,&nbsp;Aimin Wang ,&nbsp;Chunnuan Zhang","doi":"10.1016/j.anifeedsci.2025.116392","DOIUrl":"10.1016/j.anifeedsci.2025.116392","url":null,"abstract":"<div><div>Myo-inositol (MI), as a growth factor, plays a vital role in regulating the health of crustaceans. However, the optimal MI requirement of juvenile freshwater crayfish and the mechanisms that regulate growth and nutrition remain unknown. This experiment investigated the effect of MI addition on the growth, ecdysis performance, and ecdysone signaling pathway of juvenile freshwater crayfish (<em>Procambarus clarkii</em>). Six diets were prepared by adding 0 mg/kg (control group), 500 mg/kg, 1000 mg/kg, 2000 mg/kg, 3000 mg/kg, and 4000 mg/kg MI to the basal diet. A total of 360 juvenile freshwater crayfish were cultured for six weeks, in three parallel groups and their average body weight was (6.39 ± 0.05) g. Compared with the control group, the diet supplemented with 500 mg/kg and 1000 mg/kg MI significantly increased the weight gain rate (WGR) and specific growth rate (SGR) of freshwater crayfish (<em>P</em> &lt; 0.05). Using WGR and SGR as evaluation indexes, the addition of 917–942 mg/kg MI does promote the growth of freshwater crayfish. Dietary supplementation with 1000 mg/kg inositol can significantly increase the expression of ecdysone receptor (ECR) and retinoic acid X receptor (RXR) genes in the hepatopancreas and inhibit the transcription level of ecdysis inhibitory hormone (MIH) gene (<em>P</em> &lt; 0.05). Also, dietary MI enhanced digestive enzyme activity and induced the expression of genes related to the GH-IGF growth axis to promote the digestion and absorption of nutrients in freshwater crayfish. Furthermore, dietary MI reduced serum lipid molecule levels and increased serum antioxidant capacity. The results of this paper revealed the growth mechanism of juvenile freshwater crayfish in terms of digestive enzyme activities, ecdysis performance, and expression of GH-IGF axis-related genes.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116392"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary-supplemented Centella asiatica leaf extract on growth, nutrient utilization and physio-metabolic responses in GIFT fingerlings of different stocking densities reared in 10 ppt inland saline water","authors":"Potluri Sai Kishore,&nbsp;Pankaj Kumar,&nbsp;Naseemashahul Shamna,&nbsp;Parimal Sardar,&nbsp;Narottam Prasad Sahu,&nbsp;Omkar Patra,&nbsp;Jebarson Solomon,&nbsp;Saiprasad Bhusare","doi":"10.1016/j.anifeedsci.2025.116395","DOIUrl":"10.1016/j.anifeedsci.2025.116395","url":null,"abstract":"<div><div><em>Centella asiatica</em> is a medicinal plant with several antioxidant and antimicrobial properties. A 60-day feeding trial was organized to estimate the effect of dietary-supplemented <em>Centella asiatica</em> ethanolic extract on growth, nutrient utilization and physio-metabolic responses in the GIFT fingerlings of different stocking densities reared in 10 ppt inland saline water. Three iso-nitrogenous (35 % crude protein, CP) and iso-lipidic (11 %) practical diets were prepared, including graded levels (0 %, 0.5 %, 1 %) of <em>Centella asiatica</em> (CA) leaf ethanolic extract. Four hundred and five acclimated GIFT fingerlings (2.08 ± 0.02 g) with different stocking densities, S-(10, 15, 20 fingerlings/75 L) were distributed randomly among nine treatments <em>viz</em>. S₁₀CA₀, S₁₀CA_0.5, S₁₀CA₁, S₁₅CA₀, S₁₅CA_0.5, S₁₅CA₁, S₂₀CA₀, S₂₀CA_0.5, S₂₀CA₁ in triplicates. Results indicated that high stocking density significantly (<em>P &lt; 0.05</em>) lowered whole-body crude protein levels of the fish. The fishes fed with 1 % <em>Centella asiatica</em> ethanolic extract significantly (<em>P &lt; 0.05</em>) improved the weight gain (WG), thermal growth coefficient (TGC), feed conversion ratio (FCR) and protein efficiency ratio (PER) irrespective of stocking density groups. In all stocking density groups, the protease and amylase activities increased significantly (<em>P &lt; 0.05</em>) in the fishes fed with 1 % <em>Centella asiatica</em> ethanolic extract. The alanine aminotransferase (ALT), aspartate aminotransferase (AST), malate dehydrogenase (MDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT) and serum malondialdehyde (MDA) activities decreased significantly (<em>P &lt; 0.05</em>) among the fishes fed with 1 % <em>Centella asiatica</em> ethanolic extract in all stocking density groups. These results imply that the dietary inclusion of 1 % <em>Centella asiatica</em> ethanolic extract can improve growth, nutrient utilization, antioxidant activity and physio-metabolic responses in the GIFT fingerlings of different stocking densities reared in 10 ppt inland saline water.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116395"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha-lipoic acid supplementation in pike silverside microdiets: Nutrigenomic analysis reveals potential molecular pathways leading to enhanced feeding efficiency and larvae survival","authors":"Mitzi Ernestina Juárez-Gutiérrez ,&nbsp;Frida Angélica Monroy de la Peña ,&nbsp;María Gisela Ríos-Durán ,&nbsp;Carlos Cristian Martínez-Chávez ,&nbsp;Jorge Fonseca-Madrigal ,&nbsp;Sibila Concha-Santos ,&nbsp;Carlos Antonio Martínez-Palacios ,&nbsp;Pamela Navarrete-Ramírez","doi":"10.1016/j.anifeedsci.2025.116396","DOIUrl":"10.1016/j.anifeedsci.2025.116396","url":null,"abstract":"<div><div>Alpha-lipoic acid (ALA) is an antioxidant with an increasing interest in aquaculture feeds, since ALA can positively affect performance and counteract oxidative stress, it may be an ideal supplement. A previous study in pike silverside larvae (<em>Chirostoma estor</em>) showed overexpression of detrimental (stress-related) genes when larvae were fed a specific microdiet compared to those exclusively fed live food. Therefore, this study evaluated the effect of different dietary ALA supplementation levels (0, 20, 40, 80, 160 mg Kg⁻¹ diet) included on microdiets in the performance and the transcriptomic profile of 7 days post-hatched pike silverside larvae for 27 days. Dietary supplementation of alpha-lipoic acid (ALA 20 and 160 mg Kg⁻¹) halved larval mortality of pike silverside larvae and improved feeding efficiency (Food Conversion Ratio of 1.07 vs. 1.67 in the control group) in this species. Differential gene expression analysis revealed an overexpression of genes involved in the signaling pathways of mitochondrial biogenesis, reactive oxygen species response, DNA repair, and increased cellular energy, supporting survival and feeding efficiency parameters observed in pike silverside larvae. This work provides evidence of the positive effects of ALA at the lowest levels reported to date as a dietary supplement in fish.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116396"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purple bacteria as sustainable nutraceutical ingredient in aquafeed: The case of guppies","authors":"Sergio J. González Cámara ,&nbsp;Sammy Kibor ,&nbsp;Sara Olyslaegers ,&nbsp;Abbas Alloul ,&nbsp;Luis Diaz Allegue ,&nbsp;Gudrun De Boeck ,&nbsp;Siegfried E. Vlaeminck","doi":"10.1016/j.anifeedsci.2025.116394","DOIUrl":"10.1016/j.anifeedsci.2025.116394","url":null,"abstract":"<div><div>Aquafeed is essential for aquaculture and the aquatic pet industry, but its production often comes with environmental challenges. This study explores purple non-sulfur bacteria (PNSB) as a sustainable, nutrient-rich and healthy alternative to fishmeal (FM) in guppy (<em>Poecilia reticulata</em>) diets. We tested two PNSB strains, <em>Rhodobacter capsulatus</em> and <em>Cereibacter sphaeroides</em>, at 2 % and 10 % inclusion levels, comparing their effects on fish growth, reproduction, coloration, and disease resistance against the bacterial pathogen <em>Aeromonas hydrophila</em>. Control diets included a standard FM diet and one supplemented with carotenoid-rich <em>Chlorella pyrenoidosa</em>. The results were promising: PNSB diets improved fish growth and reproductive output, with PNSB at 10 % achieving the highest offspring production. Brightness, a key trait for ornamental fish marketability, was significantly enhanced in PNSB-fed guppies, boosting their visual appeal. Most strikingly, guppies on purple bacteria diets showed up to 78 % survival following <em>A. hydrophila</em> infection in a challenge test, compared to just 33 % for those on the standard FM control diet. This study highlights PNSB as a game-changing ingredient for sustainable aquafeeds, improving fish health, survival, and market value while reducing reliance on traditional FM. These findings offer an eco-friendly solution for the growing aquaculture and ornamental fish industries.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116394"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vegetable oil blends and environmental temperature influence the nutrient efficiency and gut microbiome of rainbow trout: Insight on microbial diversity, community structure, and function","authors":"H. Abdallah ,&nbsp;J.N. Idenyi ,&nbsp;A.D. Adeyemi ,&nbsp;M.F. Abanikannda ,&nbsp;D.H. Huber ,&nbsp;F. de Maria Guerrero-Toledo ,&nbsp;A.L. Gannam ,&nbsp;W.M. Sealey ,&nbsp;D.O. Igwe ,&nbsp;J.C. Eya","doi":"10.1016/j.anifeedsci.2025.116387","DOIUrl":"10.1016/j.anifeedsci.2025.116387","url":null,"abstract":"<div><div>The unsustainability and high cost of animal-based diets have increased production in the aquaculture industry, especially for carnivorous fishes like rainbow trout. This has forced farmers and researchers to look for alternatives which include the use of plant-based components. Hence, the present study evaluated the influence of dietary administration of fishmeal and vegetable oil blends (palm, camelina, and canola oil) and variation in environmental temperature on the nutrient utilization and intestinal microbiota of rainbow trout (<em>Oncorynchus mykiss</em>). Our findings demonstrated a significant impact (P &lt; 0.05) of dietary composition and temperature regimens on various nutrient efficiency parameters, including protein productive value (PPV), protein efficiency ratio (PER), lipid efficiency ratio (LER), energy productive value (EPV), and energy efficiency ratio (EER). Notably, LER was significantly influenced by dietary composition alone. 16S rRNA analysis showed that both microbial composition and diversity were affected by temperature variations while dietary composition remained stable. Taxonomic composition showed high abundance of Firmicutes, Fusobacteriota, and Proteobacteria phylum under diet and temperature conditions, with Mycoplasmataceae, Fusobacteriaceae, and Enterobacteriaceae identified as the predominant families. Fusobacteria were highly enriched at higher temperatures (18 °C and 20 °C) and reduced at lower temperatures (14 °C). Microbial functional analysis demonstrated a significant enhancement of pathways related to energy metabolism for fish fed diet 2 and propanoate metabolism for those reared at 18 °C. We infer that the combination of fishmeal and vegetable oil blends enhances nutrient retention utilization and energy metabolism in rainbow trout at elevated temperature.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116387"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of advanced chelate compounds-based 8-mineral supplement on growth performance, hematology, antioxidant status, mucus immunity, and intestinal morphology of Koi carp, Cyprinus carpio, fingerlings","authors":"Sayed Mostafa Mirtaghi ,&nbsp;Salar Dorafshan ,&nbsp;Mohammad Behzadi Tayemeh ,&nbsp;Somayeh Kalanaky ,&nbsp;Fatemeh Paykan Heyrati ,&nbsp;Mohammad Hassan Nazaran","doi":"10.1016/j.anifeedsci.2025.116385","DOIUrl":"10.1016/j.anifeedsci.2025.116385","url":null,"abstract":"<div><div>The delivery of micro-elements, crucial components of aquafeeds, can be improved through the chelation technology. Hence, this study aimed to investigate the effects of the graded levels of the chelated supplement, synthetized based on “Advanced Chelate Compounds technology”, on some physiological and immunological parameters of koi carp (<em>Cyprinus carpio</em>) fingerlings. To this end, the fish (n = 264; mean weight of 4.22 ± 0.02 g) were randomly distributed in 12 glass tanks (22 in each) and fed with the diets enriched with the supplement (Bonzafish), containing Fe, Zn, Mn, Cu, I, Cr, Co, and Se, as follows: BF0, 0; BF50, 50; BF500, 500; and BF1000, 1000 mg/kg of diet for 8 weeks. The results showed that BWG and SGR were significantly increased in fish received BF500, yet the supplementation of BF500 reduced significantly FCR. Also, the inclusion of Bonzafish statistically raised RBC counts and hematocrit levels. Moreover, the activities of GPx, SOD, and CAT significantly increased in response to the chelated supplement, especially at the dosage of 500 mg/kg. Mucosal lysozyme activities were improved by adding Bonzafish to the diets. Furthermore, the values of fold heights and intestinal diameters in the BF500 showed a significant increase compared to the other experimental groups. Enterocyte heights in BF1000 were significantly higher than the other groups. The findings suggest that the supplement could adjust the energy allocation among the various traits possibly via optimizing the metabolism. Based on the fitted nonlinear and broken-line regressions, moreover, the best dosage of Bonzafish could be predicted as 584 mg/kg. Besides, the dietary requirements of koi carps for microelements were predicted as follows: Fe, 33.61; Zn, 33.93; Mn, 15.45; Cu, 2.25; I, 1.69; Cr, 0.338; Co, 0.445; and Se, 0.114 mg/kg. Therefore, according to the results of this study, adding the advanced chelated supplement, as a metabolism optimizer, to the koi carp fingerlings diet could improve some growth and physiological parameters, which could lead to improving fish health and wellness under captivity.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116385"},"PeriodicalIF":2.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}