Animal Feed Science and Technology最新文献

{"title":"Astragalus polysaccharide and Agaricus Blazei polysaccharides improve growth performance, meat quality and health status of lambs via rescheduling the rumen microbiota","authors":"Xiongxiong Li ,&nbsp;Ting Jiao ,&nbsp;Jinlong Xu ,&nbsp;Shengguo Zhao ,&nbsp;Jianbin Liu","doi":"10.1016/j.anifeedsci.2026.116658","DOIUrl":"10.1016/j.anifeedsci.2026.116658","url":null,"abstract":"<div><div>Since the banning of antibiotics, the use of green feed additives has become crucial for the healthy farming of mutton sheep. Astragalus polysaccharides (APS) and <em>Agaricus Blazei</em> polysaccharides (ABP) exhibit various functions such as antioxidation, immunomodulation, and meat quality improvement. Therefore, this study aimed to investigate the effects of APS and ABP supplementation on growth performance, blood biochemistry, immunity, meat quality, ruminal microbiota, and rumen epithelial gene expression in lambs. Thirty-six 2.5-month-old weaned male lambs (weighing 20.85 ± 1.62 kg) were randomly divided into three groups (n = 12). They were as follows: 1) CON group (basal diet), 2) APS group (basal diet + 0.30 % APS), and 3) ABP group (basal diet + 0.20 % ABP). All lambs were reared on a large, well-managed farm in China, with each group housed in separate pens. The pre-feeding period was 10 d and the treatment period was 120 d. Dietary APS and ABP increased the final body weight, Total VFA and acetate concentration in the rumen, and serum levels of glucose (GLU), IgG, and IgM in lambs (<em>p</em> &lt; 0.05). In addition, the ADG and ruminal butyrate concentration were higher in the ABP group (<em>p</em> &lt; 0.05). Dietary ABP increased muscle C18:1n9t and unsaturated fatty acid (UFA) contents and reduced muscle drip loss, whereas APS increased C22:2 and C20:5n3 contents and decreased saturated fatty acid (SFA) contents (<em>p</em> &lt; 0.05). About ruminal microbiota, the relative abundances of unclassified_Bacteria and Spirochaetota were lower in the ABP and APS groups than in the CON group, while that of Desulfobacterota was reduced only in the ABP group (<em>p</em> &lt; 0.05). The relative abundance of <em>Rikenellaceae_RC9_gut_group</em> was decreased in both treatment groups, whereas that of <em>unclassified_Prevotellaceae</em> was decreased in the APS group (<em>p</em> &lt; 0.05). Furthermore, dietary ABP and APS increased the expression of <em>MCT1</em> and <em>NHE2</em>, both of which were positively correlated with <em>Rikenellaceae_RC9_gut_group.</em></div><div>In summary, the addition of APS and ABP to the diet can improve the growth performance, meat quality, rumen fermentation, and microbiota of lambs. It is worth noting that the overall effect of ABP is superior to that of APS.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116658"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of feeding guava (Psidium guajava L.) leaf on performance, nitrogen utilization, rumen fermentation, methane emissions mitigation, and antioxidant activity in growing lambs","authors":"Mohamed Samir Mahmoud Khalel ,&nbsp;Ayman Abdel Mohsen Hassan ,&nbsp;Ibrahim M. Khattab ,&nbsp;Mohamed Helmey Yacout ,&nbsp;Safaa E.S. Atia","doi":"10.1016/j.anifeedsci.2026.116657","DOIUrl":"10.1016/j.anifeedsci.2026.116657","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Guava leaves (GL) can serve as a new and sustainable feed source due to their rich content of various health-promoting nutrients, as well as bioactive compounds. In vitro and in vivo experiments were conducted to investigate the effects of incorporating GL into the diet of growing lambs on digestibility, nitrogen utilization, serum metabolites, rumen fermentation, methane emissions mitigation, and growth performance. Thirty male Barki lambs, with an initial average body weight (BW) of 24.56 ± 1.68 kg, were randomly assigned to three dietary groups for 150 days. Guava leaves were included at three levels: 0 % (control group, GL&lt;sub&gt;0&lt;/sub&gt;), 5 % (GL&lt;sub&gt;5&lt;/sub&gt;), and 10 % (GL&lt;sub&gt;10&lt;/sub&gt;), replacing corn silage in the diets. The phenolic compounds in GL are approximately 384.1 mg/kg of dry matter (DM). Both final BW (P = 0.007) and average daily gain (ADG) (P = 0.025) of the lambs increased linearly with higher levels of GL in their diets. Lambs fed the GL&lt;sub&gt;5&lt;/sub&gt; and GL&lt;sub&gt;10&lt;/sub&gt; diets exhibited higher final BW (P = 0.015) and ADG (P = 0.044) compared to those on the GL&lt;sub&gt;0&lt;/sub&gt; diet. There was also a linear increase in the digestibility of DM (P = 0.008), organic matter (OM) (P = 0.009), crude protein (CP) (P = 0.042), and neutral detergent fiber (NDF) (P = 0.027). There was a linear decrease in urinary nitrogen (N) excretion (P = 0.006) and a linear increase in N retention (P = 0.008). Lambs on the GL&lt;sub&gt;10&lt;/sub&gt; diet excreted less N in their urine (P = 0.018) and retained more N (P = 0.023) than those on the GL&lt;sub&gt;0&lt;/sub&gt; diet. A linear increase was also observed in ruminal pH (P = 0.002), total volatile fatty acid concentration (P = 0.004), and the molar proportion of propionate (P = 0.009), with the GL&lt;sub&gt;10&lt;/sub&gt; diet showing lower values (P &lt; 0.05) compared to the GL&lt;sub&gt;5&lt;/sub&gt; and GL&lt;sub&gt;0&lt;/sub&gt; diets. Conversely, there was a linear decrease in ammonia-N concentration (P = 0.001), the molar proportion of acetate (P = 0.001), and the acetate-to-propionate ratio (P = 0.002). A linear decrease (P = 0.000) in total gas and methane production occurred as the percentage of GL increased. The counts of protozoa decreased linearly (P = 0.009) with higher levels of GL in the diet. A linear decrease was also observed in cholesterol (P = 0.012), triglycerides (P = 0.013), and urea levels (P = 0.008), with the GL&lt;sub&gt;5&lt;/sub&gt; and GL&lt;sub&gt;10&lt;/sub&gt; diets showing lower values compared to the GL&lt;sub&gt;0&lt;/sub&gt; diet (P &lt; &lt;sub&gt;0&lt;/sub&gt;.05). Moreover, the activities of total antioxidant capacity (TAC) (P = 0.010), superoxide dismutase (SOD) (P = 0.010), and glutathione peroxidase (GSH-PX) (P = 0.005) increased linearly, while malondialdehyde (MDA) levels decreased (P = 0.022). The GL&lt;sub&gt;5&lt;/sub&gt; and GL&lt;sub&gt;10&lt;/sub&gt; diets displayed higher (P &lt; 0.05) levels of TAC, SOD, and GSH-PX, along with lower levels of MDA (P = 0.054) compared to the GL&lt;sub&gt;0&lt;/sub&gt; diet. In conclusion, GL can be utilized as a feed additive to modulate rumen fe","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116657"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activated carbon stabilises bromoform and is an effective carrier for methane mitigation in vitro","authors":"M. Myers ,&nbsp;N. Tomkins ,&nbsp;S. Payne ,&nbsp;Mozumder I ,&nbsp;A. Singh ,&nbsp;M.J. Callaghan","doi":"10.1016/j.anifeedsci.2026.116642","DOIUrl":"10.1016/j.anifeedsci.2026.116642","url":null,"abstract":"<div><div>Bromoform is a well-documented anti-methanogenic compound delivering direct abatement in ruminant production systems. However, the inherent volatility of bromoform creates challenges for use as a feed additive. This study has demonstrated the utility of an inert carrier, namely activated carbon (AC), for bromoform to achieve significant (P &lt; 0.0001) methane mitigation <em>in vitro</em>. An exponential decay model has described the reduction in methane yield over time. Of the three AC sources assessed across two bromoform concentrations (low 1.5 µM and high 2.5 µM), one AC source reduced methanogenesis by 50.3 %/d without affecting total gas (mL/24 h) or total VFA production (mmol/L). The corresponding molar % increase in propionate and decrease in acetate for this AC at the low dose resulted in a A:P of 2.2, which was significantly different (P &lt; 0.001) to the control. Aqueous isotherm data demonstrated that the same AC source had strong binding affinity to bromoform. In addition, this AC source retained bromoform at temperatures up to 80°C and displayed desorption characteristics in aqueous solution necessary for suitability as an anti-methanogenic feed additive. This binding affinity to bromoform and validation of chemical and thermal stability mitigates the health and safety risks associated with exposure to feed additives containing bromoform. Incomplete physicochemical information of the commercial AC sources limited the mechanistic understanding of the binding and release of bromoform. This study has demonstrated that application of AC as an alternative carrier for bromoform at 10–15 % (w/w) loading, can be highly advantageous in the feed additive supply chain and has potential for achieving direct methane mitigation <em>in vivo</em>.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116642"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of duration of feeding a sorghum silage-based backgrounding diet in an intense postweaning production system for Nellore bulls on growth performance","authors":"Pauliane Pucetti ,&nbsp;Julia Travassos Silva ,&nbsp;Julia Baroni Alves ,&nbsp;Livia Moreira Gandra ,&nbsp;Carina da Silva Bittencourt ,&nbsp;Isabelle Pinheiro Siqueira ,&nbsp;Luciana Navajas Renno ,&nbsp;Sidnei Antônio Lopes ,&nbsp;Mario Luiz Chizzotti ,&nbsp;Pedro Veiga Rodrigues Paulino ,&nbsp;Kendall Carl Swanson ,&nbsp;Sebastião de Campos Valadares Filho","doi":"10.1016/j.anifeedsci.2026.116669","DOIUrl":"10.1016/j.anifeedsci.2026.116669","url":null,"abstract":"<div><div>The objective of this study was to evaluate the effects of different backgrounding durations in an intense postweaning production system for Nellore bulls, as compared with immediate adaptation to a corn-based finishing diet. Forty bulls (initial BW 265 ± 5 kg, age 7 ± 1 mo) were used. Four bulls were slaughtered on day 0 for baseline empty body composition. The remaining bulls were randomly assigned to four treatments (n = 9/treatment): 0, 28, 56, or 84 days on a sorghum silage-based backgrounding diet, followed by a finishing phase (154, 126, 98, and 70 days, respectively) for a total feeding period of 154 days. The backgrounding diet consisted of 800 g/kg of sorghum silage and 200 g/kg of concentrate, and the finishing diet consisted of 200 g/kg corn silage and 800 g/kg concentrate. Both diets were provided ad libitum using electronic feed intake monitoring. Nutrient digestibility was assessed from days 14–18 during the backgrounding phase and after 28 days into the finishing phase. Bulls were weighed at the start and end of each phase, and carcass characteristics were measured after slaughter. Data were analyzed using the MIXED procedure of SAS, with Tukey’s test for mean comparisons (significance set at P ≤ 0.05 and trends considered when 0.05 &lt; P ≤ 0.10). Backgrounding duration did not affect nutrient intake, nutrient apparent digestibility, ADG, or gain efficiency (P &gt; 0.10) during the backgrounding phase. Similarly, in the finishing phase, nutrient intake, ADG, and gain efficiency were not influenced (P &gt; 0.10). However, a significant interaction between backgrounding duration and days on feed (P &lt; 0.01) was observed for daily dry matter intake during the first 28 days of the finishing phase. Increasing backgrounding duration improved the apparent digestibility of DM, OM, CP, and NFC during the finishing phase (P &lt; 0.05), with trends toward improved NDF digestibility and TDN intake (P &lt; 0.10). Final shrunk body weight (SBW), carcass weight, and carcass gain were greater in the 0-day group compared with the 28- and 56-day groups, while the 84-day group had the lowest values (P &lt; 0.05). Hot and cold dressing percentages were lower in the 84-day group (P &lt; 0.05). In conclusion, implementing backgrounding improved nutrient digestibility but did not affect ADG or gain efficiency during the finishing phase. Both 28- and 56-day backgrounding durations produced similar results for final BW, carcass weight, and gain, suggesting compensatory growth in the finishing phase.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116669"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of protein reduction in lamb fattening concentrate on in vivo digestibility, nitrogen balance and meat quality","authors":"Sandra Lobón ,&nbsp;Clàudia Baila ,&nbsp;Javier Álvarez-Rodríguez ,&nbsp;Mireia Blanco ,&nbsp;Isabel Casasús ,&nbsp;Jonathan Pelegrin-Valls ,&nbsp;Margalida Joy","doi":"10.1016/j.anifeedsci.2026.116670","DOIUrl":"10.1016/j.anifeedsci.2026.116670","url":null,"abstract":"<div><div>Determining the optimal crude protein (CP) content in light lambs’ diets is crucial for reducing ammonia. This study assessed lowering CP in fattening concentrates and its effect on digestibility and meat quality (two experiments). Two phases were tested: growing (14–19 kg BW; 18 % vs. 20 % CP) and finishing (19–25 kg BW; 17 % vs. 19 % CP). Experiment 1 involved 24 Rasa Aragonesa lambs, 12 per phase (growing: 14.0 ± 0.87 kg BW; finishing: 18.9 ± 0.78 kg BW, n = 6 per treatment) to assess apparent digestibility, nitrogen balance, and blood metabolites. The adaptation period to the diets were 12 days, while the collection period was 5 days. Digestibility was measured by total faecal collection and acid-insoluble ash (AIA) as internal marker. Experiment 2 evaluated meat quality of 24 Ripollesa lambs, slaughtered at 24.6 ± 0.23 kg BW after 42 days of fattening. Lower CP tended to decrease the acid detergent fibre (ADF) digestibility using total faecal method (P = 0.06) and decreased organic and dry matter digestibility with AIA (P = 0.03) during the growing phase (P &lt; 0.05). No differences were observed in the finishing phase (P &gt; 0.10). Digestibility coefficients were consistently underestimated with AIA compared to total faecal collection (P &lt; 0.001). Nitrogen balance was unaffected by CP level (P &gt; 0.05). Plasma metabolites were similar between treatments, except for β-hydroxybutyrate, which tended to be lower in Low CP lambs during the finishing (P = 0.09). Meat quality was minimally affected, however, Low CP increased metmyoglobin after 6 days of oxygen exposure (P = 0.005) and modified minor fatty acids. A moderate reduction of 2 % in dietary CP of light lambs appears feasible and does not impair nutrient digestibility and meat quality.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116670"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of organic microminerals (selenium, copper, and zinc) and mannan-oligosaccharides on the antioxidant and immune responses of Nile tilapia","authors":"Carolina Vasconcelos Tavares de Farias ,&nbsp;Thaise Mota Sátiro ,&nbsp;Amanda Miyuki Oshiro ,&nbsp;Amalia Pérez-Jiménez ,&nbsp;Leonardo Susumu Takahashi","doi":"10.1016/j.anifeedsci.2026.116633","DOIUrl":"10.1016/j.anifeedsci.2026.116633","url":null,"abstract":"<div><div>Individual use of organic trace minerals and mannan oligosaccharides promotes fish health. However, few studies have investigated their combined use, despite the possibility that these compounds may have additive or synergistic effects, resulting in greater benefits than their isolated application. Thus, we investigated the effect of Aquate Fish FT, a product combining selenium from yeast, peptide-chelated copper, peptide-chelated zinc, and mannan oligosaccharides, on gene expression related to the antioxidant and immune systems, liver antioxidant enzymes, leukocyte respiratory burst, total globulins, and growth performance in Nile tilapia after a four-week feeding period (Trial I). In addition, we evaluated the effects of Aquate Fish FT on liver antioxidant enzyme activities, leukocyte respiratory burst, total globulin levels, and growth performance after an eight-week feeding period (Trial II). In this second trial, fish were characterized before being subjected to an intraperitoneal challenge with endotoxin lipopolysaccharides extracted from <em>Escherichia coli</em>, administered at a dose of 500 µg kg⁻¹ in a volume of 0.2 mL per gram of fish, followed by sampling 24 h after the challenge. A total of 160 juvenile Nile tilapia (<em>Oreochromis niloticus</em>) (63.56 ± 4.41 g; mean ± SD) were fed isoproteic (30 % CP) and isoenergetic (4000 kcal GE kg⁻¹) diets containing four levels (0; 0.4; 0.8; and 1.2 %) of Aquate Fish FT and four repetition. The results showed that after four weeks, Aquate Fish FT promoted enhanced antioxidant and immune defenses, evidenced by upregulation of catalase (CAT) gene expression (P = 0.008), increased hepatic superoxide dismutase (SOD) enzyme activity (P = 0.021), upregulation of the immune cytokine interleukin 1β (IL-1β) (P = 0.002), and elevated leukocyte respiratory activity (P = 0.030). No significant differences were observed in the expression of superoxide dismutase (SOD2) (P = 0.181), glutathione peroxidase (GPx) (P = 0.192), interleukin 8 (IL-8) genes (P = 0.067), total globulins (P = 0,137), and growth performance (P &gt; 0.05). After eight weeks, Aquate Fish FT significantly increased catalase (CAT) activity, with an even greater effect following lipopolysaccharide challenge (P = 0.012). Interestingly, leukocyte respiratory burst was reduced after lipopolysaccharide administration (P = 0.012), while total globulin levels increased (P = 0.028). In this trial, there was also no difference in growth performance (P &gt; 0.05). Therefore, our results indicate that the combination of organic trace minerals (selenium, copper, and zinc) and mannan oligosaccharides (Aquate Fish FT) appears to modulate antioxidant and immunological parameters in Nile tilapia throughout different feeding periods.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116633"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardised ileal amino acid digestibility of grain legumes is comparable to soybean meal for broiler chickens","authors":"O. Hamungalu ,&nbsp;M.R. Abdollahi ,&nbsp;P.C.H. Morel ,&nbsp;S. Liu ,&nbsp;T.J. Wester","doi":"10.1016/j.anifeedsci.2026.116645","DOIUrl":"10.1016/j.anifeedsci.2026.116645","url":null,"abstract":"<div><div>The aim of the present study was to determine standardised ileal digestibility (SID) of nitrogen (N) and amino acids (AA) of faba bean, lupin, lentil, chickpea, field pea and soybean meal (SBM) for growing male broilers. Grain legumes were incorporated into experimental diets either raw or after being steam-conditioned at 80°C for 30 s. The assay diets were formulated to contain 150 g/kg crude protein with the test ingredient as the only source of protein. Meanwhile, basal endogenous AA loss was measured by using a N-free diet to estimate SID. From 21–24 days post-hatch, each diet was randomly allocated to four replicate cages (eight birds per cage) with a total of 384 Ross 308 male broiler chickens. On day 24, all birds were euthanised and ileal contents collected to determine SID. No effect (<em>P&gt;</em>0.05) was observed for heat treatment on SID of AA for lentil, lupin and field pea, but variable effects were observed in chickpea and faba bean (<em>P&lt;</em>0.05). The SID of N and all AA in SBM were not different (P&gt;0.05) to that of raw lentil, but were greater (P&lt;0.05) than that of raw field pea. No differences (P&gt;0.05) were observed for SID of N and AA between raw lupin and SBM, apart from arginine, cysteine, glutamic acid and glycine which were greater (P&lt;0.05) in lupin. In raw chickpea, only SID of isoleucine, leucine, methionine, alanine and tyrosine were lower (P&lt;0.05) than that of SBM. This study suggested that the AA digestibility coefficients of raw lupin, lentil and chickpea were not different from SBM and may be used as a replacement. However, due to their lower AA content, an additional protein source or non-bound amino acids may be needed to form a balanced diet.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116645"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid determination of residual synthetic antioxidants in dry animal feed using HPLC-DAD","authors":"Maicon Henrique Braz Garcia ,&nbsp;Patricia Daniele Silva Dos Santos ,&nbsp;Marcela De Souza Zangirolami ,&nbsp;Jacqueline Pereira Vistuba ,&nbsp;Ricardo Souza Vasconcellos ,&nbsp;Oscar de Oliveira Santos Júnior","doi":"10.1016/j.anifeedsci.2026.116638","DOIUrl":"10.1016/j.anifeedsci.2026.116638","url":null,"abstract":"<div><div>To ensure the quality and extend the shelf life of dry pet food, the industry uses synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tertiary-butylhydroquinone (TBHQ), and ethoxyquin, whose use is limited by regulatory agencies to protect animal health. This research aims to propose a new method for determining synthetic antioxidants in dry pet food. The method enables simultaneous analysis of the four most common antioxidants found in cat food and is faster and easier to apply compared to current methodologies. It involves sample preparation through grinding, vortex agitation for extraction with acetonitrile, filtration, and analysis using High-Performance Liquid Chromatography coupled with a Diode Array Detector (HPLC-DAD). The proposed method allowed the identification and quantification of the synthetic antioxidants TBHQ, BHA, ethoxyquin, and BHT in ten samples of dry cat food for adult cats, revealing maximum concentrations of up to 143.9 mg.kg⁻¹ for BHT, with low detection limits and an average R² of 0.992.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116638"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporating low levels of tannins from Acacia mearnsii can enhance in vitro rumen fermentation and reduce methane emissions without compromising nutrients digestibility","authors":"C.R. Monteiro ,&nbsp;E. Magnani ,&nbsp;M.H. Signor ,&nbsp;K.E. Loregian ,&nbsp;F. Rigon ,&nbsp;G.W. Meurer ,&nbsp;A.S. Mallmann ,&nbsp;F.S. Dalolio ,&nbsp;R.H. Branco ,&nbsp;M.A.C. Danes ,&nbsp;P.D.B. Benedeti ,&nbsp;E.M. Paula","doi":"10.1016/j.anifeedsci.2026.116637","DOIUrl":"10.1016/j.anifeedsci.2026.116637","url":null,"abstract":"<div><div>Two experiments aimed to evaluate the effects of low-level tannin inclusion in beef cattle diets on ruminal fermentation using a dual-flow continuous culture system (Exp 1) and an <em>in vitro</em> gas production (GP) system (Exp 2). In both experiments, five treatments tested the inclusion of condensed tannin extracted from <em>Acacia mearnsii</em> at 0.0, 0.8, 1.6, 3.2, and 6.4 g/kg DM in a finishing beef diet. For Experiment 1 (Exp 1), ten fermenters (1223 ± 21 mL) were arranged in a replicated 5 × 5 Latin square design over five 10-day periods, with 7 days for diet adaptation and 3 days for sample collection. In Experiment 2 (Exp 2), a 36-bottle automated <em>in vitro</em> GP system was used. The same treatments of Exp 1 were evaluated using a completely randomized design across two 48-hour fermentation batch, yielding six replicates per treatment and six blanks. In Exp 1, tannin levels did not affect digestibility parameters (P &gt; 0.21). Ammonia-N concentration and flow decreased linearly with higher tannin levels (P = 0.03). Total GP after 24 and 48 h were not affected by treatments (P &gt; 0.28). There was a quadradic effect for total VFA, CH₄ and CO₂ where the tannin level of 3.2 g/kg DM shower greater VFA concentration and lower CH₄ and CO₂ compared to the other treatments. Furthermore, tannin inclusion caused a quadratic increase in propionate (P = 0.03) and butyrate (P &lt; 0.01) concentrations, peaking at 3.2 g/kg. These findings suggest that tannin levels of 3.2 g/kg DM may be a viable strategy to modulate rumen fermentation and reduce CH₄ production. This approach could represent a possible alternative from both sustainability and energy efficiency perspectives.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116637"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of structurally distinct starches on growth, short-chain fatty acid production, hepatic lipid metabolic homeostasis, and inflammatory response in largemouth bass (Micropterus salmoides)","authors":"Han Huang ,&nbsp;Xin Liu ,&nbsp;Hao Sun ,&nbsp;Yang Xiao ,&nbsp;Yuanfa He ,&nbsp;Yongjun Chen ,&nbsp;Shimei Lin","doi":"10.1016/j.anifeedsci.2026.116655","DOIUrl":"10.1016/j.anifeedsci.2026.116655","url":null,"abstract":"&lt;div&gt;&lt;div&gt;This study compared the effects of structurally distinct starches on growth performance, intestinal microbiota, and liver health in largemouth bass to provide scientific evidence for starch utilization in aquafeeds. Three isonitrogenous and isolipidic diets (47.78 % crude protein, 10.76 % crude fat) containing distinct starch sources were formulated: common corn starch (CS), waxy corn starch (WCS), and high-amylose corn starch (HACS). Each diet was fed to triplicate groups of 20 fish (initial average weight: 8.49 g) in a recirculating aquaculture system for 65 days. The results showed that the HACS group exhibited the highest final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR), followed by the CS and WCS groups (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Furthermore, compared with the WCS group, the HACS group showed a significant increase in the concentrations of propionate and butyrate in the intestinal digesta (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Additionally, hepatic transcriptome analysis revealed that the differently expressed genes (DEGs) in the HACS and WCS groups were predominantly enriched in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to fatty acid metabolism and inflammatory regulation. Moreover, compared with the WCS group, the HACS group significantly reduced serum total cholesterol (TC) and triglyceride (TG) levels, along with fewer hepatic lipid droplets (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). This was accompanied by downregulated the mRNA expression of hepatic lipid synthesis genes including sterol regulatory element binding transcription factor 1 (srebf1), acetyl-CoA carboxylase 1 (acc1), fatty acid synthase (fasn), stearoyl-CoA desaturase (scd) (&lt;em&gt;P&lt;/em&gt; &lt; 0.05), while upregulated the mRNA expression of lipid catabolism genes such as peroxisome proliferator-activated receptor α (pparα), carnitine palmitoyltransferase 1 (cpt1), acetyl-CoA acyltransferase 2 (acaa2), adipose triglyceride lipase (atgl), hormone-sensitive lipase (hsl) and corresponding proteins (PPARα, CPT1A, ACAA2) (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Additionally, the HACS group exhibited lower serum aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (AKP) activities (&lt;em&gt;P&lt;/em&gt; &lt; 0.05), improved morphological structure and integrity of hepatocytes, downregulated the mRNA expression of pro-inflammatory factors, including interleukin-8 (il-8), interleukin-1β (il-1β), tumor necrosis factor-α （tnf-α）, and v-rel avian reticuloendotheliosis viral oncogene homolog A (nf-κb p65), and upregulated the mRNA expression of anti-inflammatory transforming growth factor-β (tgf-β&lt;em&gt;)&lt;/em&gt; (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Collectively, high-amylose diets improve the growth performance of largemouth bass, potentially mediated by increasing intestinal propionate and butyrate levels, enhancing hepatic lipid homeostasis via activating the PPARα/CPT1A/ACAA2 pathway, and alleviating hepatic inflammatory response and damage.&lt;/div&gt;&lt;","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116655"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}