Evaluation of soybean protein peptides on growth, antioxidative capacity, non-specific immunity, and disease resistance for juvenile Litopenaeus vannamei

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-09-08 DOI:10.1016/j.anifeedsci.2025.116493

Huaxing Lin , Shuqing He , Yuanming Yi , Xiaomin Zhang , Shiyu Kou , Qihui Yang , Beiping Tan , Shuyan Chi

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Abstract

This study aims to assess the effect of soybean protein peptides (SPP) on growth, antioxidative capacity, non-specific immunity, and disease resistance for Litopenaeaus vannamei. SPP was utilized to replace 10 %, 20 %, 30 %, 40 % and 50 % of fishmeal protein in basic shrimp diet, respectively. Six iso-nitrogen and iso-lipid diets, namely, FM, S10, S20, S30, S40, and S50, respectively, were designed and fed to shrimp (0.83 ± 0.01 g) for 56 d. Specific growth rate and weight gain rate in S10 group, and protein deposition rate in S10 and S20 groups were significantly higher than those in FM group (P < 0.05). The whole-body crude protein content in S10 group was significantly increased (P < 0.05). The S10 group had the highest total antioxidant capacity, whereas malondialdehyde contents in S10, S20, and S30 groups had significantly decreased (P < 0.05). Alkaline phosphatase (excluding the S20 group), acid phosphatase, phenoloxidase and lysozyme activities in S10 and S20 groups were significantly increased (P < 0.05). In S10 and S20 groups, tor, eif3k and eif4e (except in S20 group) as well as pen3a and cru (except in S10 group) mRNA expression levels significantly increased (P < 0.05). Vibrio parahaemolyticus challenge test results demonstrated a significant reduction of cumulative mortality in S10 and S20 groups (P < 0.05). Microbial diversity was significantly reduced in S40 and S50 groups (P < 0.05). There was a decrease in Bacteroidetes and Proteobacteria abundance, an increase in Firmicutes abundance, and a decrease in Vibrionaceae abundance in S10 and S20 groups. In conclusion, combined with growth, antioxidant capacity, non-specific immunity and disease resistance, it is possible to replace fishmeal with SPP in shrimp feed. Based on WGR and SPP regression analyses simulations, the optimal level of SPP replacement for fishmeal was obtained as 14.82 %.

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大豆蛋白肽对凡纳滨对虾幼虾生长、抗氧化能力、非特异性免疫和抗病能力的影响

本研究旨在探讨大豆蛋白肽（SPP）对凡纳滨对虾生长、抗氧化能力、非特异性免疫和抗病能力的影响。用SPP分别替代基础对虾饲料中10 %、20 %、30 %、40 %和50 %的鱼粉蛋白。设计鱼粉、S10、S20、S30、S40和S50 6种等氮、等脂饲料，分别饲喂（0.83 ± 0.01 g） 56 d。S10组的特定生长率、增重率以及S10和S20组的蛋白质沉积率均显著高于鱼粉组（P <； 0.05）。S10组全鱼粗蛋白质含量显著提高（P <； 0.05）。S10组总抗氧化能力最高，S10、S20和S30组丙二醛含量显著降低（P <； 0.05）。S10和S20组的碱性磷酸酶（不包括S20组）、酸性磷酸酶、酚氧化酶和溶菌酶活性显著提高（P <； 0.05）。在S10和S20组中，tor、eif3k和eif4e （S20组除外）以及pen3a和cru （S10组除外）mRNA表达量显著升高（P <； 0.05）。副溶血性弧菌攻毒试验结果显示，S10组和S20组的累积死亡率显著降低（P <； 0.05）。S40和S50组微生物多样性显著降低（P <； 0.05）。S10和S20组拟杆菌门和变形杆菌门丰度降低，厚壁菌门丰度增加，弧菌科丰度降低。综上所述，结合虾的生长、抗氧化能力、非特异性免疫和抗病能力，SPP在虾饲料中替代鱼粉是可行的。经WGR和SPP回归分析模拟，得到SPP替代鱼粉的最佳水平为14.82 %。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.