Anatomy & Cell Biology最新文献_第4页

Hair follicle stem cell-derived secretome protects astrocytes in an in vitro ischemia/reperfusion model. 毛囊干细胞来源的分泌组在体外缺血/再灌注模型中保护星形胶质细胞。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-03-18 DOI: 10.5115/acb.24.213

Fatemeh Jameie, Mehdi Dianatpour, Mahintaj Dara, Zahra Jamali, Nasrin Ghorbani, Gökhan Ünal, Mohammad Saied Salehi, Sareh Pandamooz

{"title":"Hair follicle stem cell-derived secretome protects astrocytes in an in vitro ischemia/reperfusion model.","authors":"Fatemeh Jameie, Mehdi Dianatpour, Mahintaj Dara, Zahra Jamali, Nasrin Ghorbani, Gökhan Ünal, Mohammad Saied Salehi, Sareh Pandamooz","doi":"10.5115/acb.24.213","DOIUrl":"https://doi.org/10.5115/acb.24.213","url":null,"abstract":"Ischemic stroke causes significant neuronal and glial cell damage. Recent studies suggest that stem cell-derived secretomes may offer therapeutic benefits for neural injuries. This study evaluates the protective effects of hair follicle stem cell (HFSC)-derived secretome on astrocytes subjected to oxygen-glucose deprivation (OGD), an in vitro model of ischemic stroke. In this regard, the primary astrocyte cultures were exposed to OGD conditions for 24 hours, followed by treatment with HFSC-derived secretome for 48 hours to create an environment rich in paracrine factors. The neuroprotective effect of HFSC-derived secretome on injured astrocytes was assessed using MTT assay, apoptosis flow cytometry, and qRT-PCR. The HFSC secretome mitigated cell death and apoptosis in OGD-induced astrocytes. Additionally, the secretome reduced the mRNA expression levels of pro-inflammatory cytokines IL-6, IL-1β, and TNF-α compared to the injured cells. Furthermore, it upregulated the mRNA levels of neurotrophic factors BDNF, and VEGF after OGD in astrocytes. These findings suggest that the reparative effects of the secretome are associated with astrocyte neuroprotection, anti-inflammatory properties, and anti-apoptotic effects. The neuroprotective effect of HFSC secretome may be associated with the upregulation of neurotrophic and angiogenic factors. Restored astrocytes create a conducive environment for repair, thereby expediting the recovery of impaired brain function. This study provides preclinical evidence supporting the potential of HFSC secretome in stroke therapy to improve treatment outcomes of patients who suffered from ischemic stroke.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ossification of bony nerve canals in the human head: a comparison between the endochondral and membranous bones. 人头部骨神经管的骨化：软骨内骨与膜性骨的比较。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-03-18 DOI: 10.5115/acb.24.218

Chun-Ai Li, Ji Hyun Kim, Zhe-Wu Jin, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Shogo Hayashi

{"title":"Ossification of bony nerve canals in the human head: a comparison between the endochondral and membranous bones.","authors":"Chun-Ai Li, Ji Hyun Kim, Zhe-Wu Jin, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Shogo Hayashi","doi":"10.5115/acb.24.218","DOIUrl":"https://doi.org/10.5115/acb.24.218","url":null,"abstract":"Cranial nerves and their branches pass through bony canals in the head. To reveal an ossification manner for closing the nerve canal, we examined histological sections from 32 human fetus heads at 9-18 and 28-40 weeks. Corresponding to types of bones that was penetrated by nerves, the infraorbital nerve (ION) and zygomatic nerve (ZN) were chosen for nerves passing through a membranous bone, while the maxillary nerve (MXN) and hypoglossal nerve (HGN) were chosen for nerves through the endochondral bone. The ION and ZN were sandwiched by membranous bones. The membranous bone trabeculae connected to the secondarily-developed periosteal bone and the latter partly closed the ION canal prenatally, but the closure of the ZN canal was most likely to occur postnatally. Therefore, rather than membranous bone trabeculae, the periosteal bone contributed much to closure of the nerve canal. Because it connected to the periosteum, the orbital muscle might provide a mechanical load facilitating the ossification around the ION. The MXN first passed through the cartilaginous sphenoid and, later, the perichondral bone appeared along the nerve in combination with the additional membranous ossification. Near the HGN canal, a small center of endochondral ossification appeared and the secondarily-developed bone reached the perichondrium of the canal, but the typical periosteal ossification was absent. Consequently, the perichondral or periosteal ossification along the nerve canal seemed to play a major role for a complete closure of the canal postnatally. Modern radiology might show the canal much thicker than the nerve itself in infants.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reliability of Sedillot's triangle as a potential anatomical landmark for central venous catheter insertion: insights from cadaveric study. Sedillot三角形作为中心静脉导管插入的潜在解剖学标志的可靠性：来自尸体研究的见解。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-03-05 DOI: 10.5115/acb.24.271

Apurba Patra, Anju Chaudhary, Adil Asghar, Priti Chaudhary

{"title":"Reliability of Sedillot's triangle as a potential anatomical landmark for central venous catheter insertion: insights from cadaveric study.","authors":"Apurba Patra, Anju Chaudhary, Adil Asghar, Priti Chaudhary","doi":"10.5115/acb.24.271","DOIUrl":"https://doi.org/10.5115/acb.24.271","url":null,"abstract":"Sedillot's triangle (ST), formed between the sternal and clavicular heads of sternocleidomastoid (SCM) muscle, is often used as an anatomical landmark for internal jugular vein (IJV) cannulation, but its reliability has been questioned. This cadaveric study aimed to evaluate the effectiveness of ST in locating IJV. Dissections were performed on 23 adult cadavers (46 sides). ST was exposed, and a pin was inserted at its apex to assess its relationship with IJV. Dimensions of ST and distance between apex and IJV were measured (only in cases with unsuccessful puncture), along with IJV diameter at the apex. Showed that 92.00% of sides had a fully formed ST, while 8.00% (all left-sided) lacked a gap between the SCM heads. On right side, the needle corresponded directly with IJV in 19 (82.60%) cases, but missed laterally and medially in two (8.69%) cases each. On left side, successful IJV puncture occurred in 11 (61.11%) cases, with lateral and medial misses in five (27.77%) and two (11.11%) respectively. The mean height and width of ST was 64.30±7.86 mm and 20.08±6.26 mm on right side and 63.95±7.28 mm and 15.56±9.91 mm on left side. IJV diameter at the apex was significantly higher in male and on right side. Overall, right ST proved to be a reasonably reliable landmark for successful central venous catheter. However, anatomical variability, particularly on left side, suggests that caution should be exercised, and additional methods such as ultrasound guidance may improve the accuracy and safety of IJV cannulation using this approach.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Branched ends of Reichert's cartilage in the ear: a histological study using human near-term fetuses. 耳中Reichert软骨的分支末端：一项使用人类近期胎儿的组织学研究。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-02-26 DOI: 10.5115/acb.24.220

Chun-Ai Li, Zhe-Wu Jin, Yohei Honkura, Ai Hirano-Kawamoto, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Yukio Katori

{"title":"Branched ends of Reichert's cartilage in the ear: a histological study using human near-term fetuses.","authors":"Chun-Ai Li, Zhe-Wu Jin, Yohei Honkura, Ai Hirano-Kawamoto, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Yukio Katori","doi":"10.5115/acb.24.220","DOIUrl":"https://doi.org/10.5115/acb.24.220","url":null,"abstract":"There is little information regarding whether the styloid process \"inserts\" deeply into the temporal bone petrosa. We examined the involvement of Reichert's cartilage (RC) in the petrosa using sagittal or horizontal histological sections of the heads from 17 near-term fetuses (25-40 weeks). The cranial part of RC usually carried two short branches or protrusions (T-shaped appearance). One of the branches ended in or adjacent to the future tympanic cavity, while another branch ended near the facial nerve canal. Near the latter end, a part of the RC was usually fused with the petrosa, but it was rarely separated from the latter. When a bar toward the tympanic cavity was short or absent, RC displayed a reversed J-shaped course near the facial nerve canal and ended at the attachment to the petrosa (3 of 17 specimens). Overall, considerable variations were evident in the shape, length and topographical relation of RC in the ear. Therefore, an intra-otic root of the styloid process, if identified in adults, seemed not to be a simple bar inserting between the tympanic cavity and the vertical portion of the facial nerve canal. Considerable variations in the RC head suggested that the shape was likely to be determined by mechanical loads from the developing petrosa and/or tympanic bone. Those observations were clearly contrast to the degenerating Meckel's cartilage that induces protrusions of the petrosa ant tympanic bone. After birth, endochondral ossification of the petrosa appeared to expand antero-inferiorly to involve all branches of RC.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral neuroprotective potential and toxicological profile of fascaplysin in zebrafish models. fascaplysin在斑马鱼模型中的周围神经保护潜力和毒理学分析。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-02-21 DOI: 10.5115/acb.24.290

Ki-Hoon Park, Youngbuhm Huh, Hyung-Joo Chung, Hiroyuki Konishi, Junyang Jung, Na Young Jeong

{"title":"Peripheral neuroprotective potential and toxicological profile of fascaplysin in zebrafish models.","authors":"Ki-Hoon Park, Youngbuhm Huh, Hyung-Joo Chung, Hiroyuki Konishi, Junyang Jung, Na Young Jeong","doi":"10.5115/acb.24.290","DOIUrl":"https://doi.org/10.5115/acb.24.290","url":null,"abstract":"Fascaplysin is a bioactive compound derived from marine sponges, which have anticancer properties and potential neuroprotective effects mediated by mitigation of oxidative stress-induced neurotoxicity. This study investigated the concentration-dependent effects of fascaplysin in zebrafish models, focusing on embryonic survival, cardiac function, melanocyte formation, and peripheral nerve health. Zebrafish embryos were exposed to fascaplysin at concentrations ranging from 10 nM to 100 μM, and developmental parameters were assessed. At higher concentrations (≥1 μM), fascaplysin significantly decreased embryo survival rates, delayed hatching, impaired cardiac function, and caused morphological abnormalities, including disruption of melanocyte formation and structural deformities. By contrast, lower concentrations (10 nM and 100 nM) did not exhibit significant toxicity. In adult zebrafish, fascaplysin at 100 nM reduced the expression of superoxide-producing enzymes and preserved peripheral nerve integrity following injury, as demonstrated by maintenance of fluorescence in transgenic zebrafish with expression of green fluorescent protein in Schwann cells. These findings suggest that fascaplysin exhibits peripheral neuroprotective effects at low concentrations, potentially through the reduction of oxidative stress and preservation of Schwann cell function. However, the toxicity observed at higher concentrations highlights the importance of dose optimization. Fascaplysin is a promising candidate for the development of new therapeutic strategies for peripheral neuropathies, and further studies are required to elucidate the underlying mechanisms and validate its efficacy in mammalian models.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological analysis of occipital condyle and superior articular facet of first cervical vertebra and its congruence in a Thai population. 泰国人群第一颈椎枕髁和上关节面形态分析及其一致性。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-02-18 DOI: 10.5115/acb.24.269

Suchada Treranan, Tawachai Monum, Sukon Prasitwattanaseree, Pasuk Mahakkanukrauh

{"title":"Morphological analysis of occipital condyle and superior articular facet of first cervical vertebra and its congruence in a Thai population.","authors":"Suchada Treranan, Tawachai Monum, Sukon Prasitwattanaseree, Pasuk Mahakkanukrauh","doi":"10.5115/acb.24.269","DOIUrl":"https://doi.org/10.5115/acb.24.269","url":null,"abstract":"The atlantooccipital joint, which involves the articulation between the occipital condyles (OC) at the base of the skull and the superior articular facet of the first cervical vertebra (C1), is considered a moderately reliable joint for congruence analysis. Evaluating the congruence of OC and C1 in terms of shape and measurement is critical for re-associating disarticulated cranial and postcranial elements in cases of commingled human remains. This study examined the morphological characteristics and congruence between OC and C1 in a Thai population. A total of 201 OC and C1 samples, including male and female specimens, were analyzed to classify their shapes and measurements. The most common OC shape was oval, while the C1 superior articular facet was predominantly eight-shaped. Males exhibited significantly larger OC and C1 measurements across all variables compared to females. Discriminant analysis showed an accuracy rate of 58.2%-70.1% for sex classification. Pearson's correlation coefficients for OC and C1 measurements ranged from 0.490 to 0.818, with the highest correlation observed for the maximum breadth of OC and C1. Sex-specific analysis revealed that males had the highest correlation for maximum breadth, while females showed the highest correlation for minimum breadth. The regression equation for predicting the paired size of OC and C1 had an accuracy rate of 39%-54.46%. These findings contribute to understanding craniovertebral morphology and have implications for forensic identification and surgical planning at the craniovertebral junction.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azygos lobe: exploring the bronchial supply and clinical implications. 奇叶：探讨支气管供应及其临床意义。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-02-12 DOI: 10.5115/acb.24.240

Awal M Jimah, Ashley Taylor Goldstein, Melissa McCann, Claire Stoudemire

引用次数: 0

Variant of the coracohumeralis muscle. 喙肱肌的变种。

IF 1.4

Anatomy & Cell Biology Pub Date : 2025-02-12 DOI: 10.5115/acb.24.184

Celia V Mayne, Rarinthorn Samrid, Yoko Tabira, Kazzara Raeburn, Kathleen Bubb, Joe Iwanaga, Samir Anadkat, R Shane Tubbs

引用次数: 0

Enhancing anatomy education using anatomical sign language: expanding its expressive capabilities to the upper and lower extremities, face, and brain structures. 利用解剖学手语加强解剖学教育：将其表达能力扩展到上下肢、面部和大脑结构。