Andrologia最新文献

{"title":"Implementation of World Health Organization Recommendations for Semen Analysis: A Survey of Laboratories in the United Kingdom","authors":"Lisa M Bosman,&nbsp;Iulia A Grosu,&nbsp;Peter J Ellis,&nbsp;Darren K Griffin,&nbsp;Sheryl T Homa","doi":"10.1155/2024/6137336","DOIUrl":"https://doi.org/10.1155/2024/6137336","url":null,"abstract":"<p>The standard method for identification of male fertility status is a semen analysis. This is performed in fertility and pathology laboratories accredited by different bodies in the UK such as the HFEA or UKAS, and is based on whether they perform licenced clinical treatment or diagnostic testing. The WHO laboratory semen analysis criteria provide the most comprehensive guidance for best practice, yet this is not strictly adhered to. Our objective was to determine any differences in semen analyses between laboratories in the UK, based on the regulatory body they are registered with. A cross-sectional survey was sent to NEQAS for andrology registrants (<i>n</i> = 184 laboratories), HFEA (<i>n</i> = 117 clinics), and individual ARCS members (<i>n</i> = 682). Most ARCS members are associated with NEQAS and/or the HFEA. A ∼50% laboratory response rate (<i>n</i> = 106 included responses) was found. Results were grouped based on accreditation: Group 1, UKAS accredited only (<i>n</i> = 38); Group 2, both UKAS accredited and HFEA licenced (<i>n</i> = 17); Group 3, HFEA licenced only (<i>n</i> = 42); and Group 4, no accreditation (<i>n</i> = 9). Over 85% of UKAS accredited laboratories (Groups 1 and 2) state they perform semen analysis according to WHO 2010 recommendations and adhere to best practice guidelines. A significantly fewer number of HFEA only laboratories (&lt;74% Group 3, <i>p</i>  &lt; 0.01) adhere to both guidelines. Non-HFEA laboratories (Groups 1 and 4) are almost all performing sperm counts according to WHO criteria, while &lt;60% HFEA clinics (Groups 2 and 3) perform counts according to regulation (Group 1 vs. Groups 2 and 3: Fixed sperm, <i>p</i>  &lt; 0.05; Neubauer chamber: <i>p</i>  &lt; 0.005). QC is implemented in most laboratories, however there is a significant difference (<i>p</i>  &lt; 0.01) between non-UKAS (Groups 3 and 4) and UKAS laboratories (Groups 1 and 2). There is a significant difference in semen analysis performance between UKAS and HFEA laboratories with regards to implementation of best practice guidelines and QC procedures. This may have a detrimental effect on result accuracy and consequently lead to patient misdiagnosis and mismanagement.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells","authors":"Kiana Sojoudi,&nbsp;Hossein Azizi,&nbsp;Thomas Skutella","doi":"10.1155/2024/8061627","DOIUrl":"10.1155/2024/8061627","url":null,"abstract":"<p>Krüppel-like factor 4 (<i>Klf4</i>) is a transcription factor involved in proliferation, self-renewal, differentiation, apoptosis, and somatic cell reprogramming; thus, it has a critical role in accurate spermatogenesis. In this study, we examine the different protein localizations of <i>Klf4</i> in the mouse testis and in two populations of spermatogonial stem cells (SSCs), including differentiated and undifferentiated cell types. We show that in contrast to its function during spermatogenesis in the adult testis, <i>in vitro Klf4</i> is a factor in maintaining stemness in different SSC populations. Furthermore, we suggest that its functions could be age-dependent. Bioinformatics analysis in the Cytoscape platform was conducted to highlight pathways and networks of <i>Klf4</i> interactions. Our results support the different functions of <i>Klf4</i> dependent on the age and differentiation state of SSCs.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139842448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Interplay of Varicoceles, Sperm Epigenetics, and Male Infertility: A Focused, Contemporary Review","authors":"Grace E. Sollender,&nbsp;Andre Belarmino,&nbsp;Thiago P. Furtado,&nbsp;Vadim Osadchiy,&nbsp;Jesse N. Mills,&nbsp;Matteo Pellegrini,&nbsp;Sriram V. Eleswarapu","doi":"10.1155/2024/5525839","DOIUrl":"10.1155/2024/5525839","url":null,"abstract":"<p>Despite the wide recognition of varicocele as a contributor to infertility, its exact pathophysiologic mechanism is not fully understood. As the field of male infertility continues to see progress in new diagnostic and therapeutic technologies, we sought to obtain a robust understanding of the interplay of varicoceles, oxidative stress, DNA methylation, and male infertility. We performed a comprehensive review of the contemporary literature in PubMed using search terms and keywords associated with varicoceles, DNA methylation, and male infertility. The most important findings from the literature review were included in this report. Articles were selected based on quality and relevance to the topic. Eight studies were included in this review. These studies discuss DNA methylation and varicocele-associated male infertility through evaluation of sperm parameters, DNA methylation, DNA fragmentation, and other relevant factors. Our review of the literature on varicocele-associated sperm DNA methylation indicates that global hypomethylation occurs in association with varicoceles. Some data suggest a dose-dependent effect of varicocele grade on sperm DNA methylation, with the greatest magnitude changes seen in grades II and III varicoceles. While changes in DNA methylation patterns would be expected following varicocelectomy, such changes were not found to be statistically significant in one study reviewed here. However, current human studies are limited by small sample size and short follow-up time. Future clinical and preclinical studies with robust sample sizes and hypothesis-driven investigations are necessary to further understand the relationship between DNA methylation patterns and varicocele-associated male infertility.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139852682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of YAP1 Rescues Erectile Dysfunction by Inhibiting Phenotypic Modulation through Myocardin in Diabetic Rats","authors":"Yangyan Lin,&nbsp;Jialiang Hui,&nbsp;Miaoxia Pan,&nbsp;Li Wang,&nbsp;Junqi Luo,&nbsp;Zerong Chen,&nbsp;Haibo Zhang,&nbsp;Anyang Wei","doi":"10.1155/2024/9964228","DOIUrl":"10.1155/2024/9964228","url":null,"abstract":"<p><i>Background</i>. Phenotypic modulation was shown to be related to the pathological mechanism of diabetes mellitus erectile dysfunction (DMED), and excessive expression of YAP1 was measured in the cavernous tissues of rats with DMED. <i>Objectives</i>. This investigation was performed to explore the efficiency and mechanism of gene therapy through the knockdown of YAP1 in the field of ED treatment. <i>Materials and Methods</i>. Corpus cavernosum smooth muscle cells were obtained and cultivated in vitro employing the explant technique. EdU, CCK-8, and collagen gel lattice contraction assays were subsequently performed to assess cell proliferation and contractility, which indicate smooth muscle phenotypes. <i>Results</i>. Initially, overexpression of YAP1 enhanced the proliferation and expression of YAP1 and osteopontin (OPN) while reducing cell contractility and the expression of myocardin, <i>α</i>-SMA, and calpontin, which is consistent with phenotypic modulation. Moreover, knocking down YAP1 resulted in the opposite effects, and the blockade of myocardin attenuated the reversal of phenotypic transformation. Then, 45 screened DMED rats were randomly divided into three equal groups, named streptozotocin (STZ)-induced DMED with intracavernosal injection of Ad-myocardin (DMED + myocardin), DMED with lenti-shYAP1 (DMED + shYAP1) and DMED with PBS (DMED + PBS), and 10 rats were in the negative control group. Staining with H&amp;E and Masson’s trichrome, immunochemistry, and determination of the ratio of the intracavernosal pressure/mean arterial pressure were performed to evaluate penile erection and histological changes. Western blotting was conducted to determine the molecular protein expression levels. In vivo, similar to myocardin overexpression, YAP1 inhibition rescued erectile function, repaired morphology, and maintained the contractile phenotype of smooth muscle tissues 14 days after treatment. <i>Discussion and Conclusion</i>. In conclusion, our study demonstrated the validity of gene therapy by downregulating excessive YAP1 expression to ameliorate ED in diabetic rats, and the ability of YAP1 inhibition to reverse phenotypic modulation was mediated through interaction with myocardin.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139805744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Antioxidant Humanin Analogue Restores Bull Semen Cryosurvivability","authors":"Megha Pande,&nbsp;S. K. Ghosh,&nbsp;S. Tyagi,&nbsp;R. Katiyar,&nbsp;S. Kumar,&nbsp;N. Srivastava,&nbsp;A. S. Sirohi,&nbsp; Sarika,&nbsp;N. Chand,&nbsp;S. K. Dhara,&nbsp;S. K. Bhure,&nbsp;S. Mahajan","doi":"10.1155/2024/5582359","DOIUrl":"10.1155/2024/5582359","url":null,"abstract":"<p>The current study was intended to determine the effects of humanin analogue (HNG) supplementation on semen freezability and oxidative stress parameters of cryopreserved bull spermatozoa. A total of 24 semen ejaculates, from Frieswal breeding bulls, were selected for the experiment. Samples with ≥70% progressive motility, ≥500 million spermatozoa/mL, and &gt;2 mL volume were utilized for further processing, supplementation, and cryopreservation. Each sample was divided into four equal groups and the experiment consisted of three treatment groups and one control group. In the control group, the spermatozoa were frozen in an extender without HNG, while in the other three groups, HNG supplementation was done at a concentration of 2, 10, and 20 <i>μ</i>M, respectively. The fortification of HNG at a concentration of 10 and 20 <i>µ</i>M significantly (<i>p</i>  &lt; 0.05) improved the percent post-thaw motility and kinetic variables as assessed by CASA, viability, acrosome- and plasma membrane-integrity as compared to 2 <i>µ</i>M and control group. The supplementation showed significant (<i>p</i>  &lt; 0.05) controlled oxidative stress as exhibited by a reduction in lipid peroxidation and reactive oxygen species levels. The supplemented groups also exhibited significant (<i>p</i>  &lt; 0.05) improvements in total antioxidant levels, increased mean percentage of spermatozoa exhibiting high mitochondrial membrane potential, DNA integrity, acrosome-intact noncapacitated spermatozoa, and zona binding ability. In conclusion, the supplementation of humanin analogue at 10 and 20 <i>µ</i>M significantly improved the post-thaw semen quality, functional parameters, and zona binding ability and controlled the oxidative stress in cryopreserved spermatozoa. Thus, the supplementation of its analogue may potentially prove to be a valuable approach for the improvement of semen freezability in crossbred Frieswal bulls.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139683589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Male Hyperuricemia Is Associated with Poor Reproductive Outcomes of IVF-ET","authors":"Ying Wu,&nbsp;Qian Gao,&nbsp;Jin-ran Li,&nbsp;Yuan-chen Yang,&nbsp;Min Huang,&nbsp;Xin Li,&nbsp;Xu-Hui Zeng,&nbsp;Xiao-li Sun","doi":"10.1155/2024/3923652","DOIUrl":"10.1155/2024/3923652","url":null,"abstract":"<p><i>Objective</i>. To investigate the effect of male hyperuricemia on reproductive outcome of in vitro fertilization-embryo transfer (IVF-ET). <i>Methods</i>. Clinical data of 412 couples who underwent their first cycle of IVF-ET were analyzed. According to the serum uric acid (SUA) level of the male, they were divided into control group (SUA ≤ 416 <i>μ</i>mol/L) and hyperuricemia group (SUA &gt; 416 <i>μ</i>mol/L). The primary and secondary observation indices were pregnancy outcome after quality fresh embryo transfer and embryo outcome after IVF. Binary logistic regression was used to analyze the relationship between male SUA and related pregnancy outcomes. The ROC curve of the effect of male SUA on biochemical pregnancy loss rate (BPLR) after fresh embryo transplantation was drawn. <i>Results</i>. BPLR in hyperuricemia group increased significantly than that in control group (4.7% vs. 31.6%, <i>P</i> = 0.012), and the clinical pregnancy rate and live birth rate were significantly lower (61.5% vs. 39.4%, <i>P</i> = 0.038) (56.9% vs. 33.3%, <i>P</i> = 0.027). Binary logistic regression analysis showed that BPLR after fresh embryo transfer was positively correlated with male SUA (<i>B</i> = 0.010, <i>P</i> = 0.019, OR = 1.010, 95% CI (1.002, 1.018)). The area under receiver operating characteristic curve was 0.784, the specificity was 53.7%, and the sensitivity was 100.0%, <i>P</i> = 0.010. Moreover, the total fertilization rate and 2PN fertilization rate in hyperuricemia group were significantly lower than those in control group (86.0% vs. 81.6%, <i>P</i> = 0.001) (75.0% vs. 69.6%, <i>P</i>  &lt; 0.001). <i>Conclusion</i>. Male hyperuricemia is an independent risk factor for increasing BPLR after fresh embryo transfer and can also reduce the total fertilization rate and 2PN fertilization rate of IVF.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139618801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperuricemia Induces Reproductive Damage in Male Rats through Zinc Homeostasis Imbalance and Oxidative Stress","authors":"Liger Te,&nbsp;Yuejia Li,&nbsp;Junsheng Liu,&nbsp;Xuan Liu,&nbsp;Ming Zhao,&nbsp;Shusong Wang,&nbsp;Jing Ma","doi":"10.1155/2024/6084885","DOIUrl":"10.1155/2024/6084885","url":null,"abstract":"<p>Studies have reported the adverse effects of hyperuricemia on male reproduction, but the mechanism is still unclear. The purpose of this study is to explore the mechanism of hyperuricemia on reproductive system damage in male rats. The rats were divided into a control group and a model group. Rats were given hypoxanthine and potassium oxonate dissolved in 0.5% (<i>w</i>/<i>v</i>) sodium carboxymethyl cellulose solution to induce the hyperuricemia (HUA) model. After 6 weeks, the blood, testis, and epididymis tissues of the anesthetized rats were collected for further experiments and analysis. Compared with the control rats, the serum uric acid of HUA rats was significantly increased, and the serum testosterone, sperm count, and motility were significantly decreased. The protein expression of testosterone-related molecules CYP11A1, 3<i>β</i>-HSD, and StAR was downregulated in the testis of HUA rats. The protein expression of blood–testis barrier (BTB) related molecules ZO-1 and Connexin43 was downregulated in the testis of HUA rats. Compared with the control group, the zinc content and zinc-containing enzymes (ALP and LDH) were decreased, and the mRNA and protein expression of zinc transporters (ZnT4 and ZIP7) were downregulated in the testis of HUA rats. Furthermore, the level of MDA increased and the activities of CAT, GSH-PX, and SOD decreased in the testicular tissue of HUA rats. The PI3K/AKT/mTOR pathway in the testis of HUA rats was activated. However, there was no significant change in the protein expression of autophagy associated molecules LC3, Beclin1, and ATG5 in the testis of HUA rats. Thus, the conclusion is that hyperuricemia can lead to the destruction of BTB, impaired testosterone synthesis, and decreased sperm count and motility in male rats, mainly by inducing zinc homeostasis imbalance and oxidative stress, rather than autophagy.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139618816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Properties of Pistacia vera against the Effects of Phenylhydrazine-Induced Hemolytic Anemia on Male Fertility in Mice","authors":"Soudeh Khanamani Falahati-pour,&nbsp;Arezoo Khoradmehr,&nbsp;Fatemeh Baghery,&nbsp;Fatemeh Amin,&nbsp;Najmeh Parvaz,&nbsp;Soheila Pourmasumi","doi":"10.1155/2024/5512743","DOIUrl":"10.1155/2024/5512743","url":null,"abstract":"<p><i>Objective</i>. The reproductive system can be adversely affected by anemia, characterized by elevated free radical production and lipid peroxidation. Conversely, the pistachio is well-known for its antioxidant capabilities. In this study, a mouse model was employed to investigate the safeguarding effects of the aqueous extract of pistachio (<i>Pistacia vera</i>) against oxidative stress induced by phenylhydrazine (PHZ)-triggered hemolytic anemia. <i>Materials and methods</i>. Forty-eight male mice were distributed into six distinct experimental groups (<i>n</i> = 8): control, PHZ, PHZ + 20Pis, PHZ + 80Pis, PHZ + 160Pis, and 80Pis groups. The experimental groups were administered a PHZ dose of 80 mg/kg/48 hr, along with pistachio doses of 20, 80, and 160 mg/kg/day, delivered intraperitoneally. After a period of 35 days, an assessment of sperm count, motility, morphology, and viability was conducted. Additionally, sperm chromatin quality was evaluated through Chromomycin A3, aniline blue, and toluidine blue staining. Hematoxylin–eosin staining was performed to examine the total diameter of seminiferous tubes and spermatogenic cells. Hormonal levels (FSH, LH, T) and antioxidant levels were analyzed using standard kits. <i>Results</i>. The PHZ groups exhibited a noteworthy decrease in various sperm parameters, as well as diminished chromatin quality assays and reduced levels of sex hormones when compared to the control group. Moreover, the morphometric evaluation indicated a substantial reduction in spermatogonia cell number, spermatid count, tubule count, seminiferous diameters, and seminiferous area in the PHZ group. Notably, the Pis group demonstrated the highest concentration of antioxidant contents. <i>Conclusion</i>. The protective impact of <i>P. vera</i> extract against PHZ-induced anemia on the male reproductive system in mice may be attributed to its abundant antioxidant components, which possess therapeutic properties at elevated levels.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139387183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clomiphene Citrate a First Step to Improve Idiopathic Male Infertility: A Retrospective Analysis","authors":"M. Manou Huijben,&nbsp;M. T. W. T. Tycho Lock,&nbsp;V. F. Vincent de Kemp,&nbsp;L. M. O. Laetitia de Kort,&nbsp;H. M. K. Jetske van Breda","doi":"10.1155/2023/8743882","DOIUrl":"10.1155/2023/8743882","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Infertility in men is a common and worldwide problem with limited registered treatment options and licensed therapies. Clomiphene citrate (CC) is an empirical off-label therapy. CC is a selective estrogen receptor modulator that may improve testicular function, including sperm quality, by stimulating steroidogenesis and spermatogenesis. However, there is a lack of evidence regarding the efficacy of CC as therapy for male infertility. <i>Objectives</i>. To assess the improvement of CC on sperm parameters, pregnancy, hypogonadism, and measurement of safety. <i>Methods</i>. In this retrospective study, 52 men treated with CC for idiopathic nonobstructive infertility were analyzed. Data of sperm parameters, pregnancy rate, hormonal values, side effects, improvement in hypogonadal symptoms, and potential predictors of treatment response were collected. <i>Results</i>. A total of 52 infertile men underwent CC treatment. An increase in sperm concentration was observed in 19 patients (37%). An upgrade in sperm concentration classification according to the WHO criteria was noted in 21% of the patients receiving CC therapy. Additionally, 15% of initially ineligible patients for intrauterine insemination became eligible during CC. In total, 33% of couples achieved pregnancy, either spontaneously or with assisted reproductive techniques (ART). Low-normal follicle-stimulating hormone and larger testis volume before CC treatment were predictors for better semen concentration during treatment. The retrieval rate was 75% for azoospermic patients who underwent testicular sperm extraction or percutaneous epididymal sperm aspiration, enough for intracytoplasmic sperm injection. With CC, testosterone levels increased and hypogonadal symptoms improved. Mild side effects were reported by 8% of patients. <i>Conclusion</i>. The results of this study contribute to the existing evidence that CC is a safe and noninvasive therapy for idiopathic infertile males. It could be considered as a step-up therapy before initiating more invasive ART procedures.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/8743882","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139162363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study","authors":"M. A. Setumo,&nbsp;S. S. R. Choma,&nbsp;R. Henkel,&nbsp;C. S. Opuwari","doi":"10.1155/2023/4323458","DOIUrl":"10.1155/2023/4323458","url":null,"abstract":"<div>\u0000 <p>Infertility affects about 25% of couples worldwide, and oxidative stress (OS) is linked to its idiopathic etiology. Green, black, white, and oolong teas are produced from <i>Camellia sinensis</i>, depending on their oxidation level, and contain antioxidant properties that may enhance male reproductive functions. The study aimed to investigate the effects of black tea aqueous extract on human sperm functions <i>in vitro</i>. Semen samples were collected from donors, liquefied, analyzed, and divided into normal (<i>n</i> = 40) and abnormal (<i>n</i> = 19) groups using the World Health Organization 2010 criteria. Samples were washed and incubated with black tea aqueous extracts (0, 0.4, 4, 40, 405 <i>μ</i>g/ml) for 1 hr and analyzed. Along with a considerable decrease in intracellular reactive oxygen species (ROS) production, DNA-fragmented spermatozoa, and acrosome reaction, the percentage of sperm vitality and intact mitochondrial membrane potential (MMP) increased (<i>p</i> &lt; 0.05). Furthermore, compared to the normal group, a substantial increase in the percentage of acrosome reaction, ROS production, and percentage of spermatozoa with fragmented DNA, while a reduction in the percentage of intact MMP and sperm vitality, was noted in the abnormal group (<i>p</i> &lt; 0.05). Compared to the controls, there was no significant change in motility between the normal and abnormal groups (<i>p</i> &gt; 0.05). Black tea’s antioxidant activity, caffeine concentration, or both may have contributed to its improvement in human sperm function <i>in vitro</i>.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/4323458","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138944631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}