Andrologia最新文献

{"title":"A Retrospective Study of Selective Dorsal Neurectomy for Lifelong Premature Ejaculation: Erectile and Ejaculatory Functions, Sensory Correlation, and Safety Evaluation","authors":"Yaşar Başağa,&nbsp;Ahmet Tevfik Albayrak,&nbsp;Doğukan Sökmen,&nbsp;Zülfü Sertkaya","doi":"10.1155/and/9527433","DOIUrl":"https://doi.org/10.1155/and/9527433","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Premature ejaculation (PE) is a common male sexual dysfunction and a subset of patients with lifelong PE remain unresponsive to pharmacologic and behavioral therapies. Although selective dorsal neurectomy (SDN) is practiced in certain regions, its safety and efficacy remain underexplored in Western guidelines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The main aim of this study is to evaluate the clinical outcomes, sensory changes, and safety profile of SDN in patients with lifelong PE who failed conventional treatments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this retrospective cohort study, we reviewed 82 patients with lifelong PE who underwent SDN between January and October 2024. Patients were selected based on strict clinical criteria, including failed response to medical therapy and evidence of penile hypersensitivity, which was objectively assessed using a biothesiometer. SDN involved preserving at least one dorsal penile nerve &lt;1 mm in each of the three anatomical zones. Pre and postoperative assessments included intravaginal ejaculatory latency time (IELT), the International Index of Erectile Function-5 (IIEF-5), the PE diagnostic tool (PEDT), and biothesiometry scores.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At 6 months postoperatively, mean IELT increased by 241.04 ± 134.02 s (559.12%, <i>p</i>  &lt; 0.001), PEDT scores decreased by 6.98 ± 1.66 points (48.07%, <i>p</i>  &lt; 0.001), and IIEF-5 improved by 1.51 ± 0.79 points (6.63%, <i>p</i>  &lt; 0.001). A strong negative correlation was observed between preoperative sensory thresholds and postoperative improvement in sensory thresholds (Spearman <i>r</i> = −0.83 to −0.70, all <i>p</i>  &lt; 0.001). No major complications, including glans numbness or anorgasmia, were reported.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>SDN appears to be a safe and effective surgical option for selected patients with lifelong PE who are unresponsive to conservative treatments. The procedure resulted in significant improvements in ejaculatory control with minimal risk when performed using a nerve-preserving approach.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9527433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Updated Systematic Review and Meta-Analysis of PDE-5 Inhibitors Combined With Traditional Chinese Medicine for the Treatment of Erectile Dysfunction","authors":"Yujiao Zhou,&nbsp;Xinyu Jiang,&nbsp;Baojun Guo,&nbsp;Pengyu Yang,&nbsp;Yaodong You","doi":"10.1155/and/8816246","DOIUrl":"https://doi.org/10.1155/and/8816246","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Erectile dysfunction (ED) is a common sexual dysfunction, and oral phosphodiesterase Type 5 inhibitors (PDE5Is) are the first-line treatment for the disease. Some patients with ED do not accept or benefit from conventional therapy with PDE5Is. The purpose of this study was to analyze the efficacy and safety of PDE5 inhibitors combined with traditional Chinese medicine (TCM) in the treatment of ED.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We looked for randomized controlled trials (RCTs) from China National Knowledge Infrastructure (CNKI), Wanfang Database, VIP Database, Chinese Medical Association, PubMed, Web of Science, EMBASE, and Cochrane Library, until September 14, 2024. The Cochrane Reviewer’s Handbook and Jadad scale was used to evaluate the quality of the included studies. The STATA 18.0 software was used to analyze all the data in this study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We selected 19 high-quality RCTs that included 1715 patients. Meta-analysis suggested that compared with PDE5Is alone, the combination of PDE5Is with TCM can significantly improve the erectile function index, serum testosterone level, erectile hardness grade, and total effective rate of treatment for ED patients. Also, it can reduce the ED-related discomforts without increasing adverse reactions. Additionally, the results of the publication bias test showed significant bias.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Compared with PDE5Is alone, the combined use of PDE5Is and TCM has a significant effect and is generally well tolerated. It can dramatically improve erectile function, relieve TCM syndromes, and regulate the endocrine environment, while there are no evident adverse reactions. However, more standardized, long-term, large-sample clinical trials are needed in the future to support the efficacy and safety of TCM, in order to provide more efficient alternative or complementary treatment options.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8816246","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145750933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurobiology of Premature Ejaculation: Serotonergic Mechanisms and Emerging Therapeutic Insights","authors":"Junfeng Qiu,&nbsp;Biao Wang,&nbsp;Xujun You,&nbsp;Litong Wu,&nbsp;Zhiming Hong,&nbsp;Wenbin Zhou,&nbsp;Qing Zhou","doi":"10.1155/and/2482249","DOIUrl":"https://doi.org/10.1155/and/2482249","url":null,"abstract":"<p>Premature ejaculation (PE) is the most frequently reported male sexual dysfunction, characterized by a shortened intravaginal ejaculatory latency time (IELT), lack of control over ejaculation, and psychological distress. While PE involves multifactorial psychobiological factors, accumulating evidence emphasizes the pivotal role of serotonin (5-hydroxytryptamine, 5-HT) in modulating the ejaculatory reflex. This review summarizes the principal serotonergic mechanisms regulating IELT and their clinical implications to provide a cohesive mechanistic framework. Receptor-specific pathways are briefly outlined in Section 4 and discussed in detail in Sections 5–7. We also highlight genetic polymorphisms (e.g., 5-HTTLPR, HTR1A, and HTR2C) that affect 5-HT transport and receptor function, potentially predisposing individuals to PE. The therapeutic use of selective serotonin reuptake inhibitors (SSRIs) is reviewed, with emphasis on comparative clinical efficacy and guideline-based recommendations. Despite their utility, SSRIs are associated with adverse effects such as anorgasmia, emotional blunting, and withdrawal symptoms. Emerging receptor-selective agents and next-generation 5-HT modulators are also discussed for their potential to improve efficacy and tolerability. This review provides an integrated neurobiological and clinical perspective on PE, supporting the development of targeted, individualized therapies that optimize outcomes while minimizing side effects.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/2482249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular Microbiome: May Influence Testicular Architecture and Spermatogenesis, and Predict Results of Microsurgical Testicular Sperm Extraction","authors":"Ayse Altun Akpınar,&nbsp;Murat Dursun,&nbsp;Rifat Ergul,&nbsp;Ilkay Ozdemir,&nbsp;Sibel Bulgurcuoglu Kuran,&nbsp;Ateş Kadioglu,&nbsp;Ilknur Keskin","doi":"10.1155/and/7230512","DOIUrl":"https://doi.org/10.1155/and/7230512","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>To investigate the bacterial microbiome pattern of testicular tissue and its potential role in male infertility and in predicting micro-TESE results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 180 infertile men, including nonobstructive azoospermia (NOA) cases, referred to as the study group, and men with previous unsuccessful IVF treatments and/or a sperm DNA fragmentation index of 50% or greater. NOA cases, referred to as the study group, and men with previous unsuccessful IVF treatments and/or a sperm DNA fragmentation index of 50% or greater, referred to as the control group, were enrolled in the study. The study group was also divided into micro-TESE (−) and micro-TESE (+) subgroups. Testicular tissue microbiome analysis was performed, and the results were compared between groups and subgroups of the study group. Histological changes and ZO-1 protein expression patterns were also compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Microbial composition was determined only in NOA patients. Although the composition of the microbiota was similar, micro-TESE (−) patients showed differences in phylum and genus diversity with increased abundance of the phyla Bacteroidota and Actinobacteriota, and the genera <i>Prevotella</i> and <i>Bifidobacterium</i> in one of the two subgroups. Taken together with the impaired histological and immunohistochemical findings in the micro-TESE(−) cases, these results suggest that these pathogens may play a role in testicular structural and functional changes. We performed microbiome analysis of testicular tissue using next-generation sequencing (NGS) as the primary outcome. The association of microbiome analysis with histologic changes, ZO-1 protein expression patterns, and micro-TESE results were secondary outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The testicular tissue microbiome may play a role inthe pathology of male infertility by contributing to structural and functional damageto the testis. It may predict negative micro-TESE results and may also be a specific target for supportive care approaches, such as novel antimicrobial therapies, in NOA patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7230512","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Role of Zingiber officinale Extract on Acrylamide-Induced Toxicity in Rat Testes: Immunohistochemical, Histomorphological, Biochemical, and Sperm Analysis","authors":"Hamid Reza Moradi,&nbsp;Zabihollah Khaksar,&nbsp;Fatemeh Alipour,&nbsp;Saeid Fathollahi,&nbsp;Mohammad-Reza Divar,&nbsp;Mohsen Rashidi","doi":"10.1155/and/9324806","DOIUrl":"https://doi.org/10.1155/and/9324806","url":null,"abstract":"<p>Acrylamide (ACA), a common food and industrial toxin, impairs male reproduction through oxidative stress, hormonal disruption, and apoptosis. This study evaluated the effects of <i>Zingiber officinale</i> (ginger; GING), a natural antioxidant, on testicular changes, including histomorphological, biochemical, and immunohistochemical aspects, as well as sperm quality in rats exposed to ACA. Thirty-two adult male rats were randomly divided into four groups (<i>n</i> = 8 per group): control (1 mL distilled water), ACA (10 mg/kg), GING (200 mg/kg), and ACA + GING (co-treatment). The sample size was determined based on previous toxicological studies and provided sufficient statistical power for intergroup comparisons. All treatments were administered orally once daily for 52 days. The findings demonstrated that ACA harmed sperm quality and testicular health, while GING improved them and preserved testicular structure (<i>p</i> = 0.001). GING restored testosterone, FSH, and LH levels disrupted by ACA, significantly increasing testosterone and normalizing FSH and LH concentrations to values close to the control values (<i>p</i> = 0.001). ACA increased MDA and decreased TAC, reduced BCL-2, and elevated p53 and TNF-α levels in the testes. GING reversed these effects (<i>p</i> = 0.001). Ginger extract mitigates ACA-induced testicular damage by enhancing antioxidant defenses, restoring hormonal balance, and suppressing apoptosis and inflammation, suggesting potential therapeutic benefits against ACA-related male infertility.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9324806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurobiological Insights Into Premature Ejaculation: Integrating Electroencephalography and Multimodal Techniques","authors":"Houdong He,&nbsp;Jiawei Gong,&nbsp;Yinan Lv,&nbsp;Hongzhe Xu,&nbsp;Hao Zhang,&nbsp;Tao Zhou,&nbsp;Yue Duan","doi":"10.1155/and/3869781","DOIUrl":"https://doi.org/10.1155/and/3869781","url":null,"abstract":"<p>In recent years, electroencephalography (EEG) has attracted growing attention as a noninvasive, high temporal resolution neuro-monitoring tool in the diagnosis and treatment of premature ejaculation (PE). EEG holds significant promise for elucidating the neural mechanisms underlying PE. Emerging evidence indicates that emotional regulation and psychological factors—particularly anxiety and depression—are closely associated with PE, as reflected in distinct EEG patterns. Notably, anxiety may affect the control of the ejaculatory reflex via sympathetic nervous system hyperactivity. Advanced analytical techniques, including time-frequency analysis, independent component analysis (ICA), and wavelet transforms, have provided novel insights into the brain dynamics of PE patients under various conditions. These studies have demonstrated significant alterations in EEG spectral bands during sexual stimulation and emotional fluctuations. Looking ahead, integrating EEG with other physiological signals (such as electrocardiogram [ECG] and skin conductance) and leveraging artificial intelligence algorithms could further enhance diagnostic precision and facilitate personalized therapeutic strategies for PE. This review summarizes the latest advancements in the application of EEG for PE diagnosis and treatment, while addressing the challenges and future directions for its clinical.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/3869781","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lower Serum Magnesium Levels Are Associated With Erectile Dysfunction Severity: A Cross-Sectional Study","authors":"Mesut Cilli,&nbsp;Kemal Turker Ulutas","doi":"10.1155/and/2579155","DOIUrl":"https://doi.org/10.1155/and/2579155","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Erectile dysfunction (ED) is a common condition closely associated with vascular health and endothelial function. This study aimed to evaluate the possible effects of magnesium (Mg) deficiency on ED by comparing serum Mg levels between patients diagnosed with ED and healthy individuals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This retrospective cross-sectional study was conducted on male patients, who presented to the urology outpatient clinic between June 2022 and January 2023. The study included 126 patients diagnosed with ED (Group 1) and 115 individuals without ED complaints (Group 2). The severity of ED was classified according to IIEF-5 scores.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Biochemical serum analyses revealed that the serum Mg levels of the ED group (Group 1, 1.99 ± 0.17) were significantly lower compared to the control group (Group 2, 2.11 ± 0.19, <i>p</i>  &lt; 0.001). Additionally, C-reactive protein (CRP) levels were significantly higher in the ED group than in the control group (<i>p</i>  &lt; 0.001). Receiver operating characteristic (ROC) analysis determined the best cut-off value for Mg levels in predicting ED as 1.84 mg/dL (area under the curve [AUC] = 0.515). Analysis of variance (ANOVA) test results indicated a significant difference in Mg levels among groups classified according to ED severity (<i>p</i>  &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study demonstrated that serum Mg levels were significantly lower in patients with ED compared to the control group and that there is a significant association between Mg levels and ED severity. Although these findings suggest an association between lower Mg levels and ED, causal inferences cannot be made, and the ROC analysis showed only limited discriminative ability (AUC = 0.515). Further prospective and interventional studies are warranted to clarify this relationship.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/2579155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Soybean Meal Extract (SME) Effects on Erectile Function: Alterations in Corporal Smooth Muscle and Neuronal Nitric Oxide Synthase Expression","authors":"Wei-Kung Tsai,&nbsp;Chi-Jen Chang,&nbsp;Chellappan Praveen Rajneesh,&nbsp;Chi-Feng Hung,&nbsp;Kuo-Chiang Chen,&nbsp;Xiao-Wen Tseng,&nbsp;Jai-Hong Cheng,&nbsp;Han-Sun Chiang,&nbsp;Shiu-Dong Chung,&nbsp;Yi-No Wu","doi":"10.1155/and/9404726","DOIUrl":"https://doi.org/10.1155/and/9404726","url":null,"abstract":"<p>The effects of soybean meal extract (SME) on erectile function (EF) in rats were analyzed in this study by specifically exploring changes in corpus cavernosum (CC) structure, neurophysiological biomarker levels, and hormonal dynamics. After a 14-day feeding period of 24 male juvenile rats, SME substantially decreased intracavernosal pressure (ICP) (50–55 cmH₂O) and testosterone levels (SME 100 = 1.43 ng/mL; SME 50 = 0.44 ng/mL). Histological and immunofluorescent analyses revealed a marked reduction in α-smooth muscle actin (α-SMA) and the expression of neural markers including neurofilament-1 (NF-1), neuronal nuclear (NeuN) antigen, β-III-tubulin, S100 protein, and neuronal nitric oxide synthase (nNOS) in the cavernous and dorsal penile nerves (DPNs). Western blot analysis was performed on whole penile tissue to substantiate these findings and to assess global protein expression. The results showed a dose-dependent modulation of α-SMA and downward trends in endothelial nitric oxide synthase (eNOS) and nNOS protein levels following SME treatment, confirming SME’s effect of SME on the smooth muscle phenotype and nitric oxide signaling. These results suggest that phytoestrogens in SME may disrupt hormonal balance, neuronal integrity, and vascular signaling, collectively impairing EF. This study underscores the importance of evaluating dietary SME exposure, particularly in relation to the reproductive health of young adult males.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9404726","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow Mesenchymal Stem Cells Inhibition of Prostate Cancer Progression by Exosomal MiR-486-3p","authors":"Bing-zhi Wang,&nbsp;Xiao-fei Sun,&nbsp;Min-jun Jiang,&nbsp;Jian-chun Chen,&nbsp;Peng-wei Xu","doi":"10.1155/and/2357023","DOIUrl":"https://doi.org/10.1155/and/2357023","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The global burden of prostate cancer (PC) is considerable, as it is one of the most prevalent male malignancies, posing notable challenges to treatment and prognosis. While targeted therapies have expanded therapeutic options, novel molecular strategies are still needed. This investigation assessed the impact of bone marrow mesenchymal stem cell-derived exosomes (BMMSC-Exos) enriched with microRNA-486-3p (miR-486-3p) on the malignant properties of PC cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 100 prostatectomy patients provided tumor and adjacent nontumorous prostate tissues during the period 2021–2022. BMMSC-Exos were isolated, verified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blotting, and then cocultured with human PC-3 cells. Expression of miR-486-3p was modulated using miRNA mimics and inhibitors. Functional assays, including reverse transcription quantitative polymerase chain reaction (RT-qPCR), Cell Counting Kit-8 (CCK-8), Transwell migration, and flow cytometry, were used to assess cell viability, migration, and apoptosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>BMMSC-Exos exhibited typical exosomal morphology and expressed high levels of CD63, CD81, and CD9. In comparison to matched normal tissues, miR-486-3p was notably suppressed in tumor samples, but highly enriched in BMMSC-Exos compared to BMMSCs. miR-486-3p enrichment in BMMSC-Exos led to a notable decrease in PC-3 cell proliferation and motility, along with increased apoptotic activity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Through suppression of proliferation and migration and induction of apoptosis, BMMSC-Exo-derived miR-486-3p exerts antitumor effects in PC in vitro. The observed effects suggest the potential therapeutic value of BMMSC-Exos as a delivery vehicle for miR-486-3p and provide new insights into targeted strategies for PC treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/2357023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145626023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline Neutrophil-to-Lymphocyte Ratio is Associated With Poorer Response to Low-Intensity Shockwave Therapy for Erectile Dysfunction: A Two-Centre Retrospective Cohort","authors":"Nuh Aldemir,&nbsp;İbrahim Üntan,&nbsp;Akın Demirleğen","doi":"10.1155/and/6301964","DOIUrl":"https://doi.org/10.1155/and/6301964","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Low-intensity extracorporeal shock wave therapy (Li-ESWT) is explored for erectile dysfunction (ED), yet guideline recommendations remain cautious. Simple blood-count ratios such as neutrophil-to-lymphocyte (NLR) and platelet-to-lymphocyte (PLR) may help identify who benefits.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate whether baseline NLR/PLR are associated with, and can discriminate, clinical response to Li-ESWT.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Retrospective, two-centre cohort. We analysed 160 men with ED treated with a standardized 12-session Li-ESWT protocol; 100 men without ED (5-item International Index of Erectile Function [IIEF-5] ≥22) served only for baseline comparisons and were excluded from outcome/prediction analyses. Erectile function (IIEF-5) was recorded at baseline, end of treatment, and Month 6. The primary endpoint was response at Month 6, defined a priori as the minimal clinically important difference (MCID) of <i>Δ</i>IIEF-5 ≥ 4. ROC analyses and multivariable logistic regression were adjusted for age, body mass index (BMI), hypertension, diabetes, and smoking. Preanalytic CBC screening minimized inflammatory confounding.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Response occurred in 110/160 (68.8%). NLR was higher in nonresponders than responders and controls (2.27 ± 0.71 vs. 1.63 ± 0.52 and 1.69 ± 0.60; <i>p</i>  &lt; 0.001), whereas PLR differences were smaller (<i>p</i> = 0.032). NLR discriminated responders from nonresponders with an AUC of 0.752 (95% CI 0.672–0.831); the Youden-optimal cutoff was 1.93 (sensitivity 72%, specificity 71%; PPV 85%, NPV 54). PLR showed weaker discrimination (AUC 0.610, 95% CI 0.520–0.701). In multivariable models, baseline NLR (per 1-SD) independently predicted nonresponse (OR 2.80, 95% CI 1.70–4.62; <i>p</i> &lt; 0.001), while PLR did not (OR 1.25, 95% CI 0.83–1.89; <i>p</i> = 0.29). Hypertension was also associated with nonresponse (OR 3.74, 95% CI 1.43–9.77; <i>p</i> = 0.007).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In this two-centre cohort, baseline NLR, measured on routine CBC, was associated with Li-ESWT nonresponse and provided moderate discrimination, whereas PLR performed weakly. NLR may support pretreatment risk-stratification, but prospective, sham-controlled validation and external threshold calibration are needed before clinical adoption.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6301964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}