Andrologia最新文献

{"title":"AI-Driven Evaluation of Post-COVID-19 Effects on Male Reproductive Health: A Comprehensive Analysis Beyond WHO Semen Parameters","authors":"Vinícius A. C. G. Baliza,&nbsp;Talita de O. Farias,&nbsp;Marcelo H. Furtado,&nbsp;Paulo H. R. Amaral,&nbsp;João C. B. Sepulveda,&nbsp;Victor. H. S. De Paiva,&nbsp;Maria Ivonete N. da Silva,&nbsp;Lídia M. de Andrade,&nbsp;Samyra M. S. N. Lacerda,&nbsp;Guilherme M. J. Costa,&nbsp;Juan C. González","doi":"10.1155/and/5298084","DOIUrl":"https://doi.org/10.1155/and/5298084","url":null,"abstract":"<p>The male reproductive system can be affected by viral infections, which may impact reproductive function and fertility. Although it is believed that seminal parameters generally return to normal after recovery from COVID-19, subtle changes may still affect reproductive outcomes. These changes may not be detectable using standard protocols for semen analysis outlined by the World Health Organization (WHO), requiring the use of advanced, but facile, artificial intelligence (AI) tools. This study presents the use of two different AI approaches to distinguish semen samples from COVID-19-recovered patients. The first approach focuses on analyzing sperm parameters from COVID-19-recovered men and a control group with no history of infection, though the second is based on optical ellipsometry measurements of those semen samples. Although seminal parameters of both groups fell within the normal WHO reference ranges, several machine-learning classification algorithms demonstrated significant differences between them. The Support Vector Machine (SVM) model showed the best classification performance (accuracy of 77%). At the same time, SHapley Additive exPlanations (SHAP) interpretability analysis identified progressive motility (PM) and immotile sperm (IM) as the most important parameters to separate the two groups of samples. The machine learning (ML) spectroscopy (MLS) approach showed an even better performance, with an accuracy of 90% for the SVM model. These results demonstrated that AI approaches can detect important differences in the semen of COVID-19-recovered patients that traditional seminal analysis methods fail to reveal, underscoring the potential of using AI tools to improve reproductive health assessments.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5298084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149205816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory Identification of Seminal Plasma tsRNAs in Sertoli-Cell-Only Syndrome Reveals a Pathogenic tRF-1:31-Gly-TCC-2/ZMPSTE24 Axis","authors":"Hui-Min Li,&nbsp;Jie-Yi Zhao,&nbsp;Xu-Ming Liang,&nbsp;De-Rong Li,&nbsp;Yong-Na Lan,&nbsp;Yan Gao,&nbsp;Hong-Gang Li","doi":"10.1155/and/3284495","DOIUrl":"https://doi.org/10.1155/and/3284495","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sertoli-cell-only syndrome (SCOS), the most severe form of nonobstructive azoospermia (NOA), is characterized by a complete absence of germ cells and a silent testicular microenvironment. Whether seminal plasma can mirror this pathological silence and reflect testicular spermatogenic failure remains an open question. This study investigates whether cell-free tRNA-derived small RNAs (tsRNAs) in seminal plasma may serve as both biomarkers of SCOS and mechanistic mediators in its pathogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Using high-throughput sequencing and quantitative reverse transcription polymerase chain reaction (qRT-PCR), we profiled tsRNA expression in seminal plasma from three histologically confirmed SCOS patients and three normozoospermic controls. Stability and physical forms of tsRNAs were assessed experimentally. We performed functional validation using NCCIT cells to evaluate effects on proliferation, apoptosis, and target regulation. Bioinformatic analyses identified enriched biological pathways. Independent clinical validation was obtained from a GEO dataset (GSE241326) of testicular transcriptomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We identified 22 differentially expressed tsRNAs in SCOS seminal plasma. Notably, tRF-1:31-Gly-TCC-2 was significantly upregulated and was shown to inhibit cell proliferation and promote apoptosis by directly targeting and downregulating ZMPSTE24. Concordantly, analysis of independent testicular samples confirmed ZMPSTE24 downregulation in SCOS, supporting the physiological relevance of this axis. Pathway analysis further linked these tsRNAs to spermatogenic failure and embryonic development pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study reveals that seminal plasma tsRNAs not only reflect the testicular absence of spermatogenesis in SCOS but are also functionally involved in its pathogenesis through the tRF-1:31-Gly-TCC-2/ZMPSTE24 pathway. These findings position seminal plasma as a window into testicular health and unveil a novel RNA-mediated mechanism contributing to male infertility, offering new avenues for diagnostic and therapeutic strategies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/3284495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149048480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lifestyle Intervention in Men With Abnormal Semen Analysis who Desire Fertility: A Feasibility Study","authors":"Hemapriya Mohan,&nbsp;Vishnu Priya Niranjan,&nbsp;Mahasampath Gowri,&nbsp;R. Sridhar,&nbsp;Parimala Chinta,&nbsp;Aleyamma T. K.,&nbsp;Mohan S. Kamath","doi":"10.1155/and/1689554","DOIUrl":"https://doi.org/10.1155/and/1689554","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Male factor as a sole cause contributes to 20%–30% of infertility among couples who are anxious to conceive. Lifestyle intervention trials are associated with high dropout rate and making researchers and clinicians reluctant to implement lifestyle programs for patients who may require the intervention. Current study is a feasibility study to explore the possibility of planning a large randomized controlled trial with a focused lifestyle modification that is, antioxidant-rich diet and physical activity for men with abnormal semen parameters seeking infertility.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Method&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The study is a two arm, parallel, open-label, pilot randomized controlled trial including a descriptive analysis of dropout reasons to know the reasons for dropouts. The participants included men with abnormal semen analysis who desire fertility. The intervention included focussed approach on lifestyle involving customized advice on antioxidant rich diet and exercise and a follow-up at 3 and 6 months along with fertility treatment as per protocol. The control arm was given usual care with generic advice on antioxidant rich diet as well as health promoting behavior and their follow-up was only once at 6 months. The primary outcome was participant dropout rates.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Sixty participants were randomized equally into intervention arm (&lt;i&gt;n&lt;/i&gt; = 30) and usual care control arm (&lt;i&gt;n&lt;/i&gt; = 30). No significant differences in baseline characteristics were observed across randomization groups. Among them a total of 21 participants (&lt;i&gt;n&lt;/i&gt; = 14 for intervention and &lt;i&gt;n&lt;/i&gt; = 7 in control) were available for primary outcome analysis. A total of 53.3% (16/30) with 95% CI (34.3%–71.6%) in the intervention group and 76.7% (23/30) with 95% CI (57%–90%) in the control group dropped out. The present study is a feasibility study and did not show any differences in semen parameters or DNA fragmentation indices (DFIs) between the two groups. The limitation was unequal participant contact between the arms at 3- and 6-months versus 6 months in intervention versus control arm respectively, which could have contributed to higher attrition and differential dropout in control group.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Addressing the reason for observed higher attrition in the trial is important before embarking on a larger trial to evaluate the effectiveness of individualized lifestyle intervention in men who wish to conceive.&lt;/p&gt;\u0000 \u0000 &lt;p&gt;&lt;b&gt;Trial Registration:&lt;/b&gt; C","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1689554","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149047808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment Outcomes of Microsurgical Inguinal Varicocelectomy Versus Testosterone Replacement Therapy in Male Hypogonadism by Varicocele Status","authors":"Hieu Trung Nguyen,&nbsp;Ai Le Kha Nguyen,&nbsp;Cuong Quoc Tran,&nbsp;Cuong Van Dam,&nbsp;Tin Hoang Nguyen,&nbsp;Kien Trung Nguyen","doi":"10.1155/and/8818332","DOIUrl":"https://doi.org/10.1155/and/8818332","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To compare the short-term effects of microsurgical inguinal varicocelectomy (MIV) and testosterone replacement therapy (TRT) on symptoms, serum testosterone, and semen quality in men with late-onset hypogonadism (LOH) according to varicocele status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A 3-month prospective, non-randomized, interventional cohort study was conducted in symptomatic men with testosterone deficiency and/or abnormal semen parameters. 120 patients underwent clinical, laboratory, and scrotal Doppler ultrasonographic assessment. Accordingly, 39 patients with varicocele chose MIV, 35 with varicocele chose TRT with Androgel (5 g/day), and 46 without varicocele were treated with Androgel (5 g/day). Questionnaires (ANDROTEST, ADAM, AMS, and IIEF), serum total testosterone levels, and semen analysis according to WHO 2021 criteria were assessed before and after treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ejaculatory abstinence duration demonstrated the strongest correlations with semen quality (<i>ρ</i> up to 0.52), whereas serum reproductive hormones showed only weak associations. TRT in patients without varicocele achieved the greatest improvement in the ANDROTEST and ADAM symptom domains. All treatment strategies significantly increased serum testosterone levels (all adjusted <i>p</i> &lt; 0.001). ANCOVA models showed that MIV was not associated with a higher adjusted IIEF erectile function domain score and resulted in lower adjusted serum testosterone than TRT, although the adjusted total IIEF score was higher. In contrast, MIV consistently improved semen volume, sperm concentration, total sperm count, progressive motility, total motility, vitality, and normal morphology, whereas TRT led to deterioration in several semen parameters (adjusted <i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Short-term treatment strategies for LOH produced distinct clinical benefits. TRT provided more rapid symptom relief and effectively increased serum testosterone, whereas MIV was associated with more favorable recovery of spermatogenic parameters among patients with varicocele. Hence, MIV may be considered a fertility-preserving first-line option in patients with clinical varicocele who wish to preserve fertility.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8818332","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Hesperidin in Male Infertility: A Comprehensive Review of Mechanistic Insights, Preclinical In Vivo and In Silico Evidence and Future Perspectives","authors":"Rotdelmwa Maimako Asaleye,&nbsp;Adewale Odunayo Oladipo,&nbsp;Monde A. Nyila,&nbsp;Sogolo Lucky Lebelo","doi":"10.1155/and/2069471","DOIUrl":"https://doi.org/10.1155/and/2069471","url":null,"abstract":"<p>The prevalence of male infertility is increasing at an alarming rate worldwide, impacting millions of people medically, mentally and socially. Hesperidin, a flavanone glycoside abundant in citrus, is described as a novel therapeutic agent for the amelioration of several illnesses. The mechanisms of action of hesperidin in the treatment of male infertility mainly involve restoring redox balance, alleviating inflammation and apoptosis and mitigating DNA fragmentation. The potential of hesperidin to enhance male fertility has been emphasised in some scientific research. Therefore, this article presents a comprehensive review, structured around a systematic literature search, to evaluate the current findings from in vivo and in silico preclinical models that underscore hesperidin’s potency in the management of male reproductive toxicity, the enhancement of male reproductive parameters and the mode of action underlying hesperidin’s therapeutic activity. This review thus examines the protective effects of hesperidin against chemically induced male reproductive toxicity across various animal models. Hesperidin demonstrates significant mitigation of testicular damage caused by environmental pollutants, including heavy metals (lead [Pb], mercury and aluminium), pesticides (bisphenol A [BPA]) and other toxicants. The evaluated studies employed biochemical, histological, hormonal and molecular assessments, revealing hesperidin’s capacity to reduce oxidative stress, inflammation, apoptosis and hormone disruption, thereby preserving spermatogenesis and testicular integrity. Despite promising findings, most studies focus on biochemical and histological endpoints with limited assessment of functional fertility outcomes. Future research should incorporate long-term, dose–response and functional reproductive evaluations, alongside mechanistic insights and clinical trials, to validate hesperidin’s potential as a therapeutic agent for male infertility linked to environmental toxicants.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/2069471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-Carnitine Protects Against Bisphenol A–Induced Reproductive Toxicity by Preserving Antioxidant Defense and Improving Sperm Parameters and Morphology","authors":"Darya Ahmady,&nbsp;Mohammad Ghasem Golmohammadi,&nbsp;Ramin Salimnejad,&nbsp;Shokofeh Banaei","doi":"10.1155/and/1092071","DOIUrl":"https://doi.org/10.1155/and/1092071","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Bisphenol A (BPA) is recognized as a chemical that disrupts endocrine function because it may behave as a xenoestrogen and exert estrogen-like effects on the reproductive system and interfere with and prevent the binding of natural hormones to their receptors. In contrast, L-carnitine (LC) in the male reproductive system improves sperm chromatin, regulates the function of Sertoli cells, protects sperm from oxidative damage, reduces spermatocyte apoptosis, and prevents sperm aggregation. However, few studies have focused on the effects of BPA on the reproductive system. Herein, we detected BPA-induced injuries to spermatogenesis and explored a reliable approach to counteract BPA effects. Therefore, this study examined the effect of LC on BPA-induced reproductive toxicity in mice model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Materials</h3>\u0000 \u0000 <p>A total of 40 adult male mice were assigned to five groups: control, sham, LC (100 mg/kg/day, intraperitoneal [IP]), BPA (200 mg/kg/4 times/week, IP), and BPA + LC. After 5 weeks of treatment, the right testis was removed to evaluate oxidative stress (OS)–related biomarkers, while the left testis was examined for histological analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>BPA significantly decreased sperm motility, viability, count, normal morphology, mean Johnson score (MJS), and total antioxidant capacity (TAC), while malondialdehyde (MDA) level increased significantly (<i>p</i>  &lt; 0.05). In contrast, LC treatment enhanced the sperm parameters and antioxidant defense system and improved the morphological changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>LC administration appears to mitigate the adverse effects of BPA on spermatogenesis. It effectively counteracts BPA-induced reproductive toxicity in mice and enhances the antioxidant capacity of the testis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1092071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monosodium Glutamate is Associated With Testicular Histomorphometric Alterations, Inflammation and Apoptosis in Male Wistar Rats: An Experimental Animal Study","authors":"Babatunde Oluwaseun Ibitoye,&nbsp;Adesua Emmanuel Ogunmokunwa,&nbsp;Ganiyu Oboh","doi":"10.1155/and/6118436","DOIUrl":"https://doi.org/10.1155/and/6118436","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Monosodium glutamate (MSG) is a widely used food additive, yet concerns persist regarding its potential reproductive toxicity. Previous studies have associated MSG exposure with oxidative stress, inflammation and apoptosis, which may contribute to testicular damage and impaired spermatogenesis. However, its dose-dependent effects on histological, biochemical and inflammatory parameters of the testes remain underexplored.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study investigates the dose-dependent effects of chronic MSG exposure on testicular structure, histomorphometric alterations, inflammatory markers (tumour necrosis factor-alpha [TNF-α]) and apoptotic activity (Caspase-3) in male Wistar rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Thirty male Wistar rats were assigned to five groups (<i>n</i> = 6). Group A (control) received distilled water, while Groups B, C, D and E received MSG at 30 mg/kg, 100 mg/kg, 300 mg/kg and 1000 mg/kg, respectively, for 60 days. Histological assessment, testicular protein concentration, TNF-α and Caspase-3 expression were analysed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Histological analysis revealed progressive seminiferous tubule disorganisation, germinal epithelium atrophy and expanded interstitial spaces in higher MSG doses. Combined H&amp;E and reticulin staining enabled detection of cellular changes and reticular fibre alterations not fully apparent with routine histology alone. Luminal and tubular diameters, cross-sectional area and germinal epithelium height were significantly reduced (<i>p</i> &lt; 0.05). TNF-α levels increased dose-dependently, indicating inflammation, while Caspase-3 expression was significantly elevated (<i>p</i> &lt; 0.05), suggesting increased apoptosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Chronic MSG exposure is associated with dose-dependent testicular damage, characterised by histological degeneration, inflammation and apoptosis. These changes suggest potential impairment of spermatogenic function. Public awareness and further research on protective strategies against MSG-induced testicular toxicity are recommended.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6118436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Impact of Atorvastatin on Erectile Dysfunction: A Meta-Analysis and Systematic Review”","authors":"","doi":"10.1155/and/9818691","DOIUrl":"https://doi.org/10.1155/and/9818691","url":null,"abstract":"<p>H. Su, Y. Lu, C. Ma, H. Li, and X. Su, “Impact of Atorvastatin on Erectile Dysfunction: A Meta-Analysis and Systematic Review,” <i>Andrologia</i> 54, no. 6 (2022): e14408, https://doi.org/10.1111/and.14408.</p><p>In Section 2.6, Statistical Analysis, of the above article, the text “A funnel chart was used to visualize the publication bias of the included study, which was then analysed using Begger’s and Egger’s tests. There was a substantial difference in a meta-analysis when Begger’s and Egger’s tests yielded <i>p</i> &lt; 0.05.” was incorrect.</p><p>This should have read: “A funnel chart was used to visualize the publication bias of the included study, which was then analysed using Begg’s and Egger’s tests. There was a substantial difference in a meta-analysis when Begg’s and Egger’s tests yielded <i>p</i> &lt; 0.05.”</p><p>We apologize for this error.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9818691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Analysis of the Potential Risks of Erectile Dysfunction Based on the Erection State During Masturbation: A Cross-Sectional Study","authors":"Jingjing Gao,&nbsp;Kun Wang,&nbsp;Rui Gao,&nbsp;Guosheng Xu,&nbsp;Zifang Zhang,&nbsp;Yao Zhang,&nbsp;Xiansheng Zhang","doi":"10.1155/and/6635623","DOIUrl":"https://doi.org/10.1155/and/6635623","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Erectile dysfunction (ED) has a high incidence and complex etiology. Traditional assessments, based on intercourse scenarios, often neglect erectile function during masturbation, possibly leading to misdiagnosis. This study aimed to comprehensively assess ED based on erection during masturbation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>A single-center cross-sectional study was conducted at the First Affiliated Hospital of Anhui Medical University, with the study period spanning from June 2024 to June 2025. The International Index of Erectile Function-5 (IIEF-5) scale (which was administered separately for intercourse and masturbation to capture scenario-specific function) and nocturnal penile tumescence and rigidity (NPTR) monitoring were adopted to classify the ED subtype (Group A: NPTR (abnormal) + ED during masturbation; Group B: NPTR (abnormal) + no ED during masturbation; Group C: NPTR (normal) + ED during masturbation; Group D: NPTR (normal) + no ED during masturbation). A structured questionnaire survey was implemented to gather information on potential risk factors of the study participants, followed by statistical analysis of the association.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among the 525 participants we studied, 371 suffered from ED. These patients were generally older, had a higher body mass index (BMI), a higher smoking rate, and lower physical activity levels. Subtypes differed in IIEF-5 scores, comorbidities, and related factors: Group A represents the most severe ED, and its IIEF-5 score is also the lowest (8.16 ± 3.87). Group A was associated with organic diseases; Group B with sexual desire disorder; Groups C and D with psychological disorders.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This is the first study to use masturbation erection to classify ED types, each with unique characteristics and causes, providing a basis for personalized diagnosis and treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6635623","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Effect of Extracellular Vesicles of Adipose-Derived Stem Cells on Modulation of Spermatogonial Stem Cells Function and Spermatogenesis","authors":"Mahdi Mamizadeh,&nbsp;Elaheh Amini,&nbsp;Azar Sheikholeslami,&nbsp;Mohammad Nabiuni","doi":"10.1155/and/8772965","DOIUrl":"https://doi.org/10.1155/and/8772965","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Male infertility affects a substantial portion of the population, and extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have been proposed as a cell-free therapeutic approach. This study evaluated the effects of adipose tissue-derived MSC EVs (ADSC-EVs) on spermatogonial stem cell (SSC) function in vitro and on spermatogenesis in vivo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Results</h3>\u0000 \u0000 <p>Rat ADSCs were cultured to passage 3, and EVs were isolated from serum-free conditioned medium by ultracentrifugation (100,000 ×<i>g</i>, 70 min). In vitro, SSCs co-cultured with sertoli cells were treated with 10, 50, and 100 µg/mL ADSC-EVs; cell viability was measured by MTT after 4 days and gene expression (SYCP3, DAZL) assessed by qRT-PCR after 10 days. In vivo, immature male Wistar rats (21 days old, <i>n</i> = 6 per group) were randomly assigned to control or intratesticular injection of ADSC-EVs at 0.25, 1.25, or 2.5 mg/kg; testes were collected 10 days postinjection for histological (hematoxylin and eosin [H&amp;E]) assessment and qRT-PCR analysis. In vitro, 10 µg/mL ADSC-EVs produced the highest SSC viability (78.06%) and significantly upregulated SYCP3 (~5.3-fold, <i>p</i> &lt; 0.001) while DAZL showed a nonsignificant change. In vivo, low-dose ADSC-EVs (0.25 mg/kg) increased the presence of spermatozoa within the lumen of seminiferous tubules and moderately upregulated SYCP3 (fold change ~1.95), whereas ADSC-EVs at 2.5 mg/kg induced seminiferous epithelium degeneration and absence of mature spermatozoa. DAZL expression was downregulated in vivo at the tested doses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>ADSC-EVs exert dose-dependent, dual effects on spermatogenesis: at low concentrations, they promote SSC differentiation (SYCP3-associated), while higher concentrations can damage seminiferous architecture and impair sperm release. These findings emphasize the importance of dose optimization for potential therapeutic applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8772965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148616028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}