Andrologia最新文献

{"title":"Comparison of ICSI Outcomes Between Microfluidic and Conventional Sperm Selection Methods: A Systematic Review and Meta-Analysis","authors":"Tohid Rezaei Topraggaleh,&nbsp;Hosein Azizi,&nbsp;Amir Fattahi,&nbsp;Ehsan Dadkhah,&nbsp;Behrooz Niknafs","doi":"10.1155/and/9210663","DOIUrl":"https://doi.org/10.1155/and/9210663","url":null,"abstract":"<div>\u0000 <p>In recent years, intracytoplasmic sperm injection (ICSI) has become the main selected option in more than half of the assisted reproductive technologies (ARTs) cycles conducted each year. Selecting the most competent sperm cells with highest fertilization potential is crucial to improve clinical outcomes following ICSI procedure. Microfluidic (MF) chips have emerged as a new alternative to conventional methods for selecting high-quality spermatozoa with minimal DNA fragmentation. However, due to the lack of consensus about the effects of MF sperm selection on ICSI outcomes, in the present study, we systematically reviewed and meta-analyzed the impact of MF on fertilization rate (FR), embryo quality (EQ), euploidy rate (ER), pregnancy rate (PR), and live birth rate (LBR) following ART procedures. A systematic comprehensive literature search of the PubMed, Scopus, and Web of Science databases was conducted to determine the impact of MF on fertility outcomes compared to conventional methods. Meta-analysis using random effects model was used to estimate pooled odds ratios (ORs) and weighted mean differences (WMDs) for various ART outcome measures. The search identified 1714 records, of which 21 articles were included in the review. MF significantly increased the rate of fertilization (OR = 1.04; 95% CI: 1.00–1.07; and WMD = 7.24, 95% CI: 6.93, 7.91) and EQ (OR = 1.44; 95% CI: 1.30–1.60), while nonsignificantly increasing the ER (OR = 1.20; 95% CI: 0.92–1.58), PR (OR = 1.09; 95% CI: 0.94–1.25), and LBR (OR = 1.27; 95% CI: 0.99–1.62). Moreover, subgroup analyses revealed that MF chips resulted in significantly higher FR and improved EQ in unexplained infertile and higher PR in male factor infertility patients. These results indicate that couples with a history of male factor or unexplained infertility may benefit from MF–based sperm selection.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9210663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Four MicroRNA Expressions (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in the Seminal Plasma of Asthenozoospermic Infertile Tunisian Patients","authors":"Rihab Derbel,&nbsp;Imen Belguith,&nbsp;Rim Sakka,&nbsp;Salima Daoud,&nbsp;Afifa Sellami,&nbsp;Hassen Kammoun,&nbsp;Leila Ammar Keskes","doi":"10.1155/and/8882390","DOIUrl":"https://doi.org/10.1155/and/8882390","url":null,"abstract":"<div>\u0000 <p>Asthenozoospermia (ASZ) is a semen condition characterized by reduced sperm motility; it is observed in ~40% of male infertility cases, and it is related to various factors including genetic and epigenetic factors such as microRNAs (miRNAs). Although miRNAs play a crucial role in spermatogenesis, their function in seminal plasma and their relationship with ASZ remain largely unknown, with a considerable amount of controversial and contradictory data. This study aimed to explore the expression variation of miRNAs (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in relation to ASZ. We collected seminal plasma samples from Tunisian infertile patients with ASZ (progressive sperm motility &lt;32%) and without ASZ (normospermic patients) and performed quantitative real-time PCR to identify differentially expressed miRNAs. Three miRNAs showed dysregulated expression in the seminal plasma of patients with ASZ (miR-34c-5p, miR-146b-5p, and miR-199a-3p). Two miRNAs were upregulated (miR-34c-5p and miR-146b-5p; <i>p</i> &lt; 0.05), while miR-199a-3p was downregulated (<i>p</i> &lt; 0.05) compared to patients without ASZ. The expression levels of miR-199a-3p and miR-34c-5p were significantly correlated with sperm motility and vitality percentage with an area under the receiver operating characteristic (ROC) curve (area under the curve [AUC]) of 0.75 and 0.71, respectively. These findings indicate that the three miRNAs (miR-34c-5p, miR-146b-5p, and miR-199a-3p) are implicated in the etiology of ASZ and could be recognized as potential indicators of disrupted sperm function and male infertility.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8882390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and Clinical Applications of Testicular Prostheses","authors":"Feng Zhang,&nbsp;Shuai Gao,&nbsp;Linna Xie,&nbsp;Tailang Yin,&nbsp;Yanwei Sha","doi":"10.1155/and/5760800","DOIUrl":"https://doi.org/10.1155/and/5760800","url":null,"abstract":"<div>\u0000 <p>The absence of a testicle can cause significant physical and psychological trauma in men. Testicular prosthesis implantation, as a critical treatment option, not only restores the normal appearance of the scrotum but also significantly improves patients’ quality of life. This paper reviews the historical development, material evolution, and current clinical applications of testicular prostheses and explores future trends. Continuous advancements in the materials and manufacturing processes of conventional testicular prostheses have gradually improved their biocompatibility and patient satisfaction. The application of tissue engineering and 3D printing technologies introduces new possibilities for the customization and functionalization of testicular prostheses. Although current testicular prostheses primarily aim to restore appearance, future functional prostheses with controlled testosterone release may reduce patients’ dependence on exogenous testosterone supplementation. This review also discusses in detail the indications for testicular prosthesis implantation, the selection of surgical timing, and perioperative management, providing valuable references for clinical practice.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5760800","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Cymbopogon citratus Hydroalcoholic Extract and Vitamin E Coadministration in Alleviating Complications From Testicular Ischemia–Reperfusion-Induced Injury in Rats","authors":"Kambiz Yousefi,&nbsp;Mahdi Farid,&nbsp;Elham Moghtadaei Khorasgani,&nbsp;Mehrdad Yadegari","doi":"10.1155/and/4028767","DOIUrl":"https://doi.org/10.1155/and/4028767","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Impaired circulation caused by testicular torsion can lead to infertility and testicular damage. Factors such as increased production of free radicals and inflammatory cytokines as well as damage to blood vessels may play a role in the development of postreperfusion injury. Compounds containing antioxidants may inhibit or reduce complications caused by testicular torsion by neutralizing free radical activity.</p>\u0000 <p><b>Objectives:</b> This study investigates the evaluation of the therapeutic efficacy of <i>Cymbopogon citratus</i> hydroalcoholic extract (CCHE) and vitamin E coadministration against complications caused by testicular torsion.</p>\u0000 <p><b>Methods:</b> Thirty male Wistar rats were allocated into five groups of six: (1) negative control; (2) positive control (sham); (3) intraperitoneally (CCHE); (4) vitamin E intramuscularly; and (5) coadministration of CCHE and vitamin E. Testicular ischemia was induced in groups 2, 3, 4, and 5. Subsequently, the treatment was performed on groups 3, 4, and 5 for 3 weeks daily. Sections of testes were collected on day 21. Superoxide dismutase (SOD) and catalase (CAT) were assessed by real-time PCR, and their activities were analyzed. Moreover, a histopathological evaluation was also performed.</p>\u0000 <p><b>Results:</b> Coadministration of CCHE and vitamin E significantly reduced the complications caused by testicular torsion.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4028767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in Premature Ejaculation Research From 2004 to 2023: A Bibliometric Analysis","authors":"Dongyue Ma,&nbsp;Hao Wang,&nbsp;Elena Colonnello,&nbsp;Andrea Sansone,&nbsp;Chunlin Wang,&nbsp;Hui Zhang,&nbsp;Anmin Wang,&nbsp;Hongyuan Chang,&nbsp;Jiutian Yang,&nbsp;Jun Guo,&nbsp;Fu Wang","doi":"10.1155/and/9984316","DOIUrl":"https://doi.org/10.1155/and/9984316","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Premature ejaculation (PE) is a largely prevalent sexual complaint that can lead to distress in couple relationships and can significantly reduce patients’ quality of life. Despite the considerable progress in PE research in the last decades, there is still a lack of visual and summarized literature for comprehensive understanding.</p>\u0000 <p><b>Objective:</b> This study aims to identify collaboration patterns among different authors, countries, and institutions in PE research and explore the hotspots and prospects in the field.</p>\u0000 <p><b>Methods:</b> On January 22, 2024, we conducted a search of all relevant literature in the Web of Science Core Collection (WoSCC) database from January 1, 2004, to December 31, 2023, and supplemented the search with PubMed database for author publication volumes. Visual analyses of authors and keywords were performed using the bibliometrix R package, CiteSpace, and VOSviewer software.</p>\u0000 <p><b>Results:</b> In the field of PE, 5457 authors contributed to 1760 articles published across 442 journals. The number of publications gradually increased from 2004 to 2023, with a relatively stable trend in the past 5 years. The <i>Journal of Sexual Medicine</i> (340 papers) had the highest number of publications, and positive collaboration was observed among countries and institutions. Several core author groups emerged, including Jannini EA (60 WoSCC/67 PubMed papers), Waldinger MD (59 WoSCC/90 PubMed papers), and Rowland DL (51 WoSCC/68 PubMed papers), contributing significantly to the literature. The United States was the major contributor, with 384 papers. Keyword cluster analysis revealed that research focuses on PE diagnosis, pharmaceutical treatment (e.g., selective serotonin reuptake inhibitors [SSRIs] and antidepressants), etiology (e.g., serotonin [5-HT], NOS, and vas deferens), and relationships with prostate, thyroid, anxiety, depression, and other risk factors.</p>\u0000 <p><b>Conclusion:</b> Based on the knowledge map, this study provides an intuitive representation of the recent 20 years of PE research, offering a preliminary analysis of major research teams and exploring research hotspots, frontiers, and trends. This analysis aims to guide researchers and serve as a reference for future research on PE.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9984316","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vim3 and Mxi-2 as Promising New Molecular Biomarkers for the Diagnosis of Sperm Disorders","authors":"Richard Weiten,&nbsp;Jan Herden,&nbsp;Manuel Huerta,&nbsp;Tim Nestler,&nbsp;Johan Denil,&nbsp;Axel Heidenreich,&nbsp;Enno Storz,&nbsp;Melanie von Brandenstein","doi":"10.1155/and/1233135","DOIUrl":"https://doi.org/10.1155/and/1233135","url":null,"abstract":"<div>\u0000 <p><b>Introduction:</b> Male infertility remains a significant clinical challenge that requires the exploration of new diagnostics strategies. Truncated proteins, such as Vim3, a truncated variant of vimentin, show promise as a new class of biomarkers for diagnosing fertility disorders. Our objective was to assess the potential utility of Vim3 and Mxi-2, a shortened iteration of mitogen-activated protein kinase (MAPK) p38, as noninvasive biomarkers for diagnosing sperm disorders.</p>\u0000 <p><b>Methods:</b> Semen samples of 19 fertile males (control group) and 82 infertile males with semen abnormalities and no female factor (<i>n</i> = 28 OAT [oligo–astheno–teratozoospermia] syndrome, <i>n</i> = 8 azoospermia, <i>n</i> = 29 teratozoospermia, <i>n</i> = 11 astheno–teratozoospermia, and <i>n</i> = 6 oligozoospermia) were analyzed using ELISA (enzyme-linked immunosorbent assay) and flow cytometry. Furthermore, we conducted a lateral flow assay (LFA) specific to Vim3.</p>\u0000 <p><b>Results:</b> Our analysis revealed that Vim3 and endothelin-1 (ET-1) expression levels were significantly downregulated in non-normozoospermic patients (e.g., OAT syndrome), while Mxi-2 was overexpressed in these patients compared to the control group (<i>p</i> &lt; 0.01). Additionally, we introduced a novel LFA that provides a user-friendly and effective method for examining Vim3 as a biomarker in clinical settings.</p>\u0000 <p><b>Conclusion:</b> Our results suggest that Vim3 and Mxi-2 have potential as noninvasive semen biomarkers for diagnosing sperm disorders.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1233135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shilajit Mitigates Diabetes-Induced Testicular Dysfunction in Mice: A Modulation in Insulin Sensitivity, Germ Cell-Junctional Dynamics, and Oxido-Apoptotic Status","authors":"Arti Rajpoot,&nbsp;Ajai Kumar Pandey,&nbsp;Vikas Kumar Roy,&nbsp;Raghav Kumar Mishra","doi":"10.1155/and/5517176","DOIUrl":"https://doi.org/10.1155/and/5517176","url":null,"abstract":"<div>\u0000 <p>Diabetes is a chronic metabolic condition that causes testicular damage by high oxidative stress, rendering 35% of afflicted people infertile. Shilajit is a traditional Indian medicine known for its antioxidant, antidiabetic, and aphrodisiac properties. However, its effectiveness on diabetes-induced testicular dysfunction remains unclear. Therefore, the current investigation aimed to determine whether Shilajit could restore testicular functions in diabetic mice. Two days postpartum male Parkes mice received a single intraperitoneal injection of streptozotocin (STZ) (90 mg/kg BW) to induce diabetes. Three months postinjection, the effects of daily Shilajit (100 and 200 mg/kg BW) treatment were evaluated for one spermatogenic cycle in adult diabetic mice, using Empagliflozin (10 mg/kg BW) as a positive control. In STZ-induced diabetic mice, testicular functions were compromised due to disruptions in testosterone biosynthesis, changes in germ-cell ratios, and increased oxidative stress and apoptosis. Shilajit restored glycemic status in diabetic mice by significantly decreasing serum glucose, insulin level, and homeostasis model assessment of insulin resistance (HOMA-IR) value while increasing insulin sensitivity. These effects were comparable to those observed with conventional antidiabetic medication Empagliflozin. Further, Shilajit stimulates steroidogenesis and germ cell dynamics of diabetic mice by increasing the activity of StAR, 3<i>β</i>-HSD, and 17<i>β</i>-HSD enzymes and 1C:2C, 4C:S-Ph, and 1C:4C germ cell ratios, respectively. Shilajit also improves blood–testis barrier (BTB) functioning by increasing expression of ZO-1, Connexin-43, N-Cadherin, and <i>β</i>-catenin as well as oxidative status and apoptosis by modulating NF-E2–related factor 2 (Nrf-2)/heme oxygenase 1 (HO-1) signaling and Bax/Bcl-2 ratio. Subsequently, Shilajit improved the histoarchitecture of testis and epididymis in diabetic mice and recovered both qualitative and quantitative sperm parameters, as seen by higher percentages of sperm motility, viability, and normal sperm morphology as well as increased sperm numbers in cauda epididymis. In summary, Shilajit restores glycemic status, increases insulin sensitivity, stimulates steroidogenesis, and improves testicular functions through Sertoli cell and Nrf-2/HO-1 signaling in STZ-induced diabetic mice.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5517176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship of Early Embryonic Development In Vitro Fertilization With Sperm Zinc Homeostasis and Seminal Plasma Antioxidation","authors":"Meiyang Du,&nbsp;Yizhen Hu,&nbsp;Dan Yang,&nbsp;Shiwei Luan,&nbsp;Xiaowei Luo,&nbsp;Haoyang Dai,&nbsp;Yasong Geng,&nbsp;Bo Zheng,&nbsp;Shusong Wang","doi":"10.1155/and/6663110","DOIUrl":"https://doi.org/10.1155/and/6663110","url":null,"abstract":"<div>\u0000 <p>The study aims to explore the sperm parameters that affect early embryo development in in vitro fertilization (IVF) and to study their relationship with sperm zinc homeostasis and seminal plasma antioxidation. The semen samples were incubated at 37°C for 1, 2, 3, and 4 h, and the sperm progressive motility (PR) was analyzed. We found that the sperm PR significantly decreased after 3 h of incubation (<i>p</i> &lt; 0.01) and further decreased after 4 h (<i>p</i> &lt; 0.001). The correlation analysis showed that sperm PR was significant positively correlated with transferable embryo rate (<i>r</i> = 0.155, <i>p</i> = 0.026) and blastocyst formation rate (<i>r</i> = 0.140, <i>p</i> = 0.044) after incubation for 4 h, while its decline rate was significant negatively correlated with transferable embryo rate (<i>r</i> = −0.151, <i>p</i> = 0.030) and blastocyst formation rate (<i>r</i> = −0.220, <i>p</i> = 0.002). Additionally, the ELISA assay indicated that patients with PR &gt; 32% and decline rate &lt; 30% had significantly higher levels of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in the seminal plasma compared to patients with PR &lt; 32% and decline rate &gt; 30% (<i>p</i> &lt; 0.05), while malondialdehyde (MDA) levels were significantly lower (<i>p</i> &lt; 0.05). The results of the zinc fluorescence probe showed that the level of free zinc ion in sperm in the high motility and low decline rate group was significantly higher than that in the low motility and high decline rate group (<i>p</i> &lt; 0.05). The quantitative real-time polymerase chain reaction (qRT-PCR) and immunofluorescence results showed that the group with high motility and low decline rate had significantly higher relative expression levels of G protein-coupled receptor 39 (GPR39), anoctamin 1 (ANO1), and zinc transporter protein 13 (ZIP13) mRNA (<i>p</i> &lt; 0.05). In conclusion, patients with low PR and high PR decline rate after 4 h of incubation had poor early embryo development results in IVF. This may be through the reduction of zinc homeostasis-associated protein expression to accelerate the oxidative stress process, thereby reducing sperm quality and ultimately affecting embryonic development.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6663110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annona muricata Aqueous Extract Ameliorates Testicular and Epididymal Sperm Toxicity in Rats Exposed to Gasoline Fumes","authors":"Ogechukwu E. Ezim,&nbsp;Naboth E. Agabus,&nbsp;Eugene N. Onyeike,&nbsp;Sunny O. Abarikwu","doi":"10.1155/and/4797178","DOIUrl":"https://doi.org/10.1155/and/4797178","url":null,"abstract":"<div>\u0000 <p>In the present study, aqueous leaf extract of <i>Annona muricata</i> was assessed for its capacity to mitigate testicular damages and sperm toxicity induced by gasoline fumes. Thirty-two male Wistar rats were randomized into four groups of eight animals per group: the control group was given distilled water, and the PEBI group was exposed to petrol by inhalation and petrol-exposed animals that were concomitantly treated with <i>A. muricata</i> at 100 and 200 mg kg⁻¹ body weight (b.w), respectively (PEBI + AM100 and PEBI + AM200) via oral gavage. Oxidative stress markers, sperm quality variables and histological examination of the testes evaluated at the end of the 10-week exposure period showed that coadministration of <i>A. muricata</i> significantly reduced thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H₂O₂) concentration, elevated catalase (CAT) activity, diminished DNA fragmentation and improved sperm quality, thereby attenuating testicular and epididymal sperm toxicity in rats exposed to gasoline fumes. Interestingly, the higher dose <i>A. muricata</i> could also lower lipid peroxidation to below the control values, demonstrating the antilipoperoxidative and antioxidant protective effects of <i>A. muricata</i> in a gasoline fume model of testicular and spermatoxicity in rats.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4797178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin Ameliorates Hydrocortisone-Induced Leydig Cell Injury and Testicular Dysfunction by Upregulating the Nrf2 Pathway","authors":"Jingjing Guo,&nbsp;Feifei Ma,&nbsp;Yang Li,&nbsp;Renshan Ge,&nbsp;Anshi Wu","doi":"10.1155/and/5596226","DOIUrl":"https://doi.org/10.1155/and/5596226","url":null,"abstract":"<div>\u0000 <p>Curcumin has antifragmentation, antivirus, anti-infection, and antioxidation properties. Curcumin has been proven to prevent the peroxidation of sperm and testis membranes, thus enhancing sperm motility and reducing sperm abnormalities. This study aims to explore the role of curcumin in improving hydrocortisone (HCT)-induced Leydig cell injury by upregulating the Nrf2 pathway. Thirty male rats were divided into three groups: control group (CG, <i>n</i> = 10), model group (MG, <i>n</i> = 10), and curcumin treatment group (CTG, <i>n</i> = 10). CG rats were fed 2.5 mL/kg carboxymethyl cellulose as a blank control. MG rats were fed with 10 mg/kg HCT for 2 weeks. CTG rats were fed with 10 mg/kg HCT for 2 weeks and 100 mg/kg curcumin orally every day for 4 consecutive weeks. The ultrastructural score of Leydig cells was higher in the MG than in the CG (<i>p</i> &lt; 0.05) and was lower in the CTG than in the MG (<i>p</i> &lt; 0.05). The levels of Nrf2 and HO-1 were lower in the MG than in the CG (<i>p</i> &lt; 0.05) and higher in the CTG than in the MG (<i>p</i> &lt; 0.05). Curcumin can improve the biochemical and histological structural damage of rat testes induced by HCT by upregulating Nrf2 expression. This protective effect relies on the Nrf2 pathway inhibiting oxidative stress in the body, increasing testosterone levels and enzyme activity, thereby improving testicular dysfunction caused by interstitial cell damage.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5596226","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143117888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}