Andrologia最新文献

{"title":"The Effects of miR-425-3p and Seminal Microbiota on Semen Parameters: Insights Into Male Infertility","authors":"Osamah Batiha,&nbsp;Raghad Abu-Diak,&nbsp;Qutaiba Ababneh,&nbsp;Mahmoud A. Alfaqih,&nbsp;Mothanna Nawafleh,&nbsp;Raghad Abu Al-Rub,&nbsp;Masood Abu-Halima","doi":"10.1155/and/6262703","DOIUrl":"https://doi.org/10.1155/and/6262703","url":null,"abstract":"<div>\u0000 <p>Male infertility is a multifaceted condition influenced by various factors, resulting in diverse phenotypic expressions. MicroRNAs (miRNAs), oxidative stress, and microbiota are potential contributors to compromised semen quality. This study explores the relationship between miR-425-3p, seminal microbiota, and oxidative stress in relation to impaired semen parameters. Semen samples were collected from 39 individuals with sperm motility &lt;20% and 31 individuals with sperm motility &gt;20%. Microbial cultures were performed to analyze the microbiota in the samples. DNA extraction from seminal cultured colonies, followed by PCR amplification targeting the 16S rRNA gene was conducted for sequencing, while RNA extraction from seminal plasma facilitated RT-qPCR analysis of miR-425-3p. Additionally, oxidative lipid degradation was evaluated using a thiobarbituric acid reactive species (TBARS) assay, comparing malondialdehyde (MDA) levels between samples with good and poor sperm motility. Our analysis revealed a diverse semen microbiota, predominantly consisting of Staphylococcus, Enterococcus, Bacillus, Micrococcus, and Neisseria. Less prevalent genera included <i>Streptococcus, Acinetobacter, Corynebacterium, Actinomyces, and Escherichia coli</i>. Higher bacterial loads were positively correlated with improved sperm motility, averaging 20,373 CFU/mL in the group with sperm motility &gt;20% compared to 1247 CFU/mL in group with sperm motility &lt;20% (<i>p</i> = 0.03). Conversely, miR-425-3p expression was significantly elevated in group with sperm motility &lt;20%, showing a fourfold increase (<i>p</i> = 0.034). Reduced miR-425-3p expression was observed in samples abundant in Bacillus, particularly when Neisseria was absent. MDA levels, a marker of oxidative stress, were significantly higher in the less motile group (9.46 ± 5.07 nmol/mL) compared to the motile group (5.25 ± 3.32 nmol/mL, <i>p</i> = 0.038). The analysis revealed distinct microbial profiles associated with varying sperm motility levels. These findings establish a connection between increased miR-425-3p expression, elevated seminal oxidative stress, and decreased sperm motility. Our data provide insights into the roles of seminal microbiota and microRNA in the pathogenesis of impaired semen quality.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6262703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are Uncertain Vasectomy Pathology Reports Suggestive of Procedural Failure? Long-Term Data From a Large Cohort","authors":"Max D. Sandler,&nbsp;Laura Angulo-Llanos,&nbsp;Rohan Dureja,&nbsp;Adriana Sandino,&nbsp;Faaris Khan,&nbsp;Veronica Junco,&nbsp;Dan V. Tran,&nbsp;Julio Yanes,&nbsp;Adam D. Williams,&nbsp;Thomas A. Masterson","doi":"10.1155/and/6054225","DOIUrl":"https://doi.org/10.1155/and/6054225","url":null,"abstract":"<div>\u0000 <p><b>Purpose:</b> During vasectomy, technical errors such as difficulty identifying and transecting the vas deferens may occur. Pathology reports indicating an incompletely transected vas may create uncertainty regarding procedural success. However, there is a paucity of large cohort studies investigating this correlation. To address this gap, we explored the relationship between abnormal pathology reports and vasectomy failure rates to determine whether such reports should impact clinical decision making.</p>\u0000 <p><b>Methods:</b> We retrospectively reviewed charts of men aged ≥18 who underwent vasectomy between 2004–2024. Abnormal pathology reports failed to identify complete vas deferens cross sections. Vasectomy failure was defined as presence of motile sperm or ≥100,000 nonmotile sperm on postvasectomy semen analysis (PVSA) ≥3 months.</p>\u0000 <p><b>Results:</b> Of 2446 vasectomies with pathology reports, 2399 (98.1%) reports were normal, 1330 returned for PVSA, and 55 were failures (4.1%). Conversely, 47 (1.9%) had an abnormal report. Of these, 31 patients returned for PVSA, 87.1% (<i>n</i> = 27) were successes with failure rate of 9.7% (<i>n</i> = 3), and one patient had sperm on PVSA &lt;3 months after vasectomy but was lost to follow-up. The three failures were secondary to errors in transecting the vas on one side. Pathology reports stated “benign nerve and fibrovascular tissue,” “artery,” and “no vas deferens lumen visualized.”</p>\u0000 <p><b>Conclusions:</b> Outcomes from a large cohort over two decades suggests although pathology provides early feedback, abnormal reports do not reliably predict vasectomy failure. Pathology should not independently guide clinical decision making without confirmatory semen analysis, which remains the marker of success. Future research could investigate outcomes of patients with abnormal pathology who did not follow up for semen analysis.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6054225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Handling of Human Ejaculate Affects Osmolality and Yield by Density Gradient Centrifugation and Possibly Also Sperm DNA Integrity","authors":"Emma Holmes,&nbsp;Petr Houska,&nbsp;Lars Björndahl,&nbsp;Ulrik Kvist","doi":"10.1155/and/4015844","DOIUrl":"https://doi.org/10.1155/and/4015844","url":null,"abstract":"<div>\u0000 <p>During routine handling in the assisted reproductive technology (ART) laboratory, the osmolality of the ejaculate increases, and live spermatozoa adapt to this increase. Preparation media are generally isotonic, having an osmolality of ~290 mOsm/kg. Therefore, when the ejaculate is placed on a density gradient, the spermatozoa are exposed to a hypotonic challenge. The aim of this study was to investigate whether osmotic challenges affect the yield of density gradient centrifugation (DGC), the DNA fragmentation index (%DFI), and the proportion of high DNA stainable sperm (%HDS). Furthermore, it was investigated if these effects could be counteracted by dilution of the ejaculate. Semen samples from healthy donors were collected and used after written consent. Four protocols of different experimental conditions were followed. These included storage at 37°C, storage at room temperature (RT), dilution after liquefaction, and ejaculation directly into a buffer. Pairwise comparisons of the studied aliquot and its own control were performed. Storage at 37°C led to an increase in osmolality to 352 mOsm/kg and halved the yield to 4.5%. Storage at RT led to an increase in osmolality to 384 mOsm/kg and halved the yield to 5.8%. The %DFI and %HDS did not change during these storage conditions. In undiluted samples, osmolality increased to 373 mOsm/kg and in diluted to 323 mOsm/kg. The %DFI and %HDS did not change. After ejaculation into a buffer, the osmolality increased only to 315 mOsm/kg after storage and the yield halved after storage to 12.9%. The %DFI was significantly lower after ejaculation into a buffer compared to dilution after liquefaction, 6% versus 11%, respectively. No difference was observed in %HDS. The challenges to sperm function induced by osmolality changes, and possibly also sperm DNA integrity, can be counteracted by collecting ejaculates directly into an isotonic buffer.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4015844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ameliorative Effects of Probiotics on the Sperm Quality and Testicular Structure After Ischemia/Reperfusion Injury Following Testicular Torsion/Detorsion","authors":"Zohresadat Akbari,&nbsp;Narges Karbalaei,&nbsp;Saied Karbalay-Doust,&nbsp;Seyed Shahram Shekarforoush,&nbsp;Farhad Koohpeyma,&nbsp;Sourena Rastin","doi":"10.1155/and/8508956","DOIUrl":"https://doi.org/10.1155/and/8508956","url":null,"abstract":"<div>\u0000 <p>Oxidative stress is a critical contributor to the pathophysiology of ischemia/reperfusion (I/R) injury associated with testicular torsion (TT) and detorsion (D) (TT/D). This study examines the antioxidative properties of the probiotics Lactobacillus (L.) reuteri and Lactobacillus (L.) rhamnosus in ameliorating TT/D-induced oxidative and histological damage. Male Sprague–Dawley rats (<i>n</i> = 6 per group) were allocated into five experimental groups: a sham-operated group, a torsion/detorsion (T/D) group, and three T/D groups treated with <i>L. reuteri</i> (T/D+LRe), <i>L. rhamnosus</i> (T/D+LRm), or a combination of both (T/D+LRe+LRm). TT was induced by rotating the right testis 720° clockwise for 2 h, followed by 60 days of reperfusion. The testis was then excised for further analysis. The evaluated parameters included sperm quality, hormone levels (FSH, LH, and testosterone), as well as levels of malondialdehyde (MDA) and glutathione (GSH). Additionally, the activities of myeloperoxidase (MPO) catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as histological changes, were assessed. The findings demonstrated that TT/D significantly increased the FSH and LH levels, reduced sperm quality and testosterone levels, elevated MDA levels, and enhanced MPO activity. Additionally, TT/D reduced the GSH levels and the activities of GPx, CAT, and SOD. Probiotic treatment, particularly with <i>L. rhamnosus</i>, significantly increased testicular GSH levels and the activities of antioxidant enzymes GPx and CAT while reducing MDA levels and MPO activity. Histopathological analysis revealed severe testicular damage, including reductions in spermatogenic, sertoli, and Leydig cells following TT/D, mitigated by <i>L. rhamnosus</i>. These results suggest that probiotics, especially <i>L. rhamnosus</i>, may protect against I/R injury in a rat model of testicular T/D, likely due to their antioxidant properties.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8508956","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of ICSI Outcomes Between Microfluidic and Conventional Sperm Selection Methods: A Systematic Review and Meta-Analysis","authors":"Tohid Rezaei Topraggaleh,&nbsp;Hosein Azizi,&nbsp;Amir Fattahi,&nbsp;Ehsan Dadkhah,&nbsp;Behrooz Niknafs","doi":"10.1155/and/9210663","DOIUrl":"https://doi.org/10.1155/and/9210663","url":null,"abstract":"<div>\u0000 <p>In recent years, intracytoplasmic sperm injection (ICSI) has become the main selected option in more than half of the assisted reproductive technologies (ARTs) cycles conducted each year. Selecting the most competent sperm cells with highest fertilization potential is crucial to improve clinical outcomes following ICSI procedure. Microfluidic (MF) chips have emerged as a new alternative to conventional methods for selecting high-quality spermatozoa with minimal DNA fragmentation. However, due to the lack of consensus about the effects of MF sperm selection on ICSI outcomes, in the present study, we systematically reviewed and meta-analyzed the impact of MF on fertilization rate (FR), embryo quality (EQ), euploidy rate (ER), pregnancy rate (PR), and live birth rate (LBR) following ART procedures. A systematic comprehensive literature search of the PubMed, Scopus, and Web of Science databases was conducted to determine the impact of MF on fertility outcomes compared to conventional methods. Meta-analysis using random effects model was used to estimate pooled odds ratios (ORs) and weighted mean differences (WMDs) for various ART outcome measures. The search identified 1714 records, of which 21 articles were included in the review. MF significantly increased the rate of fertilization (OR = 1.04; 95% CI: 1.00–1.07; and WMD = 7.24, 95% CI: 6.93, 7.91) and EQ (OR = 1.44; 95% CI: 1.30–1.60), while nonsignificantly increasing the ER (OR = 1.20; 95% CI: 0.92–1.58), PR (OR = 1.09; 95% CI: 0.94–1.25), and LBR (OR = 1.27; 95% CI: 0.99–1.62). Moreover, subgroup analyses revealed that MF chips resulted in significantly higher FR and improved EQ in unexplained infertile and higher PR in male factor infertility patients. These results indicate that couples with a history of male factor or unexplained infertility may benefit from MF–based sperm selection.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9210663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Four MicroRNA Expressions (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in the Seminal Plasma of Asthenozoospermic Infertile Tunisian Patients","authors":"Rihab Derbel,&nbsp;Imen Belguith,&nbsp;Rim Sakka,&nbsp;Salima Daoud,&nbsp;Afifa Sellami,&nbsp;Hassen Kammoun,&nbsp;Leila Ammar Keskes","doi":"10.1155/and/8882390","DOIUrl":"https://doi.org/10.1155/and/8882390","url":null,"abstract":"<div>\u0000 <p>Asthenozoospermia (ASZ) is a semen condition characterized by reduced sperm motility; it is observed in ~40% of male infertility cases, and it is related to various factors including genetic and epigenetic factors such as microRNAs (miRNAs). Although miRNAs play a crucial role in spermatogenesis, their function in seminal plasma and their relationship with ASZ remain largely unknown, with a considerable amount of controversial and contradictory data. This study aimed to explore the expression variation of miRNAs (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in relation to ASZ. We collected seminal plasma samples from Tunisian infertile patients with ASZ (progressive sperm motility &lt;32%) and without ASZ (normospermic patients) and performed quantitative real-time PCR to identify differentially expressed miRNAs. Three miRNAs showed dysregulated expression in the seminal plasma of patients with ASZ (miR-34c-5p, miR-146b-5p, and miR-199a-3p). Two miRNAs were upregulated (miR-34c-5p and miR-146b-5p; <i>p</i> &lt; 0.05), while miR-199a-3p was downregulated (<i>p</i> &lt; 0.05) compared to patients without ASZ. The expression levels of miR-199a-3p and miR-34c-5p were significantly correlated with sperm motility and vitality percentage with an area under the receiver operating characteristic (ROC) curve (area under the curve [AUC]) of 0.75 and 0.71, respectively. These findings indicate that the three miRNAs (miR-34c-5p, miR-146b-5p, and miR-199a-3p) are implicated in the etiology of ASZ and could be recognized as potential indicators of disrupted sperm function and male infertility.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8882390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and Clinical Applications of Testicular Prostheses","authors":"Feng Zhang,&nbsp;Shuai Gao,&nbsp;Linna Xie,&nbsp;Tailang Yin,&nbsp;Yanwei Sha","doi":"10.1155/and/5760800","DOIUrl":"https://doi.org/10.1155/and/5760800","url":null,"abstract":"<div>\u0000 <p>The absence of a testicle can cause significant physical and psychological trauma in men. Testicular prosthesis implantation, as a critical treatment option, not only restores the normal appearance of the scrotum but also significantly improves patients’ quality of life. This paper reviews the historical development, material evolution, and current clinical applications of testicular prostheses and explores future trends. Continuous advancements in the materials and manufacturing processes of conventional testicular prostheses have gradually improved their biocompatibility and patient satisfaction. The application of tissue engineering and 3D printing technologies introduces new possibilities for the customization and functionalization of testicular prostheses. Although current testicular prostheses primarily aim to restore appearance, future functional prostheses with controlled testosterone release may reduce patients’ dependence on exogenous testosterone supplementation. This review also discusses in detail the indications for testicular prosthesis implantation, the selection of surgical timing, and perioperative management, providing valuable references for clinical practice.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5760800","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Cymbopogon citratus Hydroalcoholic Extract and Vitamin E Coadministration in Alleviating Complications From Testicular Ischemia–Reperfusion-Induced Injury in Rats","authors":"Kambiz Yousefi,&nbsp;Mahdi Farid,&nbsp;Elham Moghtadaei Khorasgani,&nbsp;Mehrdad Yadegari","doi":"10.1155/and/4028767","DOIUrl":"https://doi.org/10.1155/and/4028767","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Impaired circulation caused by testicular torsion can lead to infertility and testicular damage. Factors such as increased production of free radicals and inflammatory cytokines as well as damage to blood vessels may play a role in the development of postreperfusion injury. Compounds containing antioxidants may inhibit or reduce complications caused by testicular torsion by neutralizing free radical activity.</p>\u0000 <p><b>Objectives:</b> This study investigates the evaluation of the therapeutic efficacy of <i>Cymbopogon citratus</i> hydroalcoholic extract (CCHE) and vitamin E coadministration against complications caused by testicular torsion.</p>\u0000 <p><b>Methods:</b> Thirty male Wistar rats were allocated into five groups of six: (1) negative control; (2) positive control (sham); (3) intraperitoneally (CCHE); (4) vitamin E intramuscularly; and (5) coadministration of CCHE and vitamin E. Testicular ischemia was induced in groups 2, 3, 4, and 5. Subsequently, the treatment was performed on groups 3, 4, and 5 for 3 weeks daily. Sections of testes were collected on day 21. Superoxide dismutase (SOD) and catalase (CAT) were assessed by real-time PCR, and their activities were analyzed. Moreover, a histopathological evaluation was also performed.</p>\u0000 <p><b>Results:</b> Coadministration of CCHE and vitamin E significantly reduced the complications caused by testicular torsion.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4028767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in Premature Ejaculation Research From 2004 to 2023: A Bibliometric Analysis","authors":"Dongyue Ma,&nbsp;Hao Wang,&nbsp;Elena Colonnello,&nbsp;Andrea Sansone,&nbsp;Chunlin Wang,&nbsp;Hui Zhang,&nbsp;Anmin Wang,&nbsp;Hongyuan Chang,&nbsp;Jiutian Yang,&nbsp;Jun Guo,&nbsp;Fu Wang","doi":"10.1155/and/9984316","DOIUrl":"https://doi.org/10.1155/and/9984316","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Premature ejaculation (PE) is a largely prevalent sexual complaint that can lead to distress in couple relationships and can significantly reduce patients’ quality of life. Despite the considerable progress in PE research in the last decades, there is still a lack of visual and summarized literature for comprehensive understanding.</p>\u0000 <p><b>Objective:</b> This study aims to identify collaboration patterns among different authors, countries, and institutions in PE research and explore the hotspots and prospects in the field.</p>\u0000 <p><b>Methods:</b> On January 22, 2024, we conducted a search of all relevant literature in the Web of Science Core Collection (WoSCC) database from January 1, 2004, to December 31, 2023, and supplemented the search with PubMed database for author publication volumes. Visual analyses of authors and keywords were performed using the bibliometrix R package, CiteSpace, and VOSviewer software.</p>\u0000 <p><b>Results:</b> In the field of PE, 5457 authors contributed to 1760 articles published across 442 journals. The number of publications gradually increased from 2004 to 2023, with a relatively stable trend in the past 5 years. The <i>Journal of Sexual Medicine</i> (340 papers) had the highest number of publications, and positive collaboration was observed among countries and institutions. Several core author groups emerged, including Jannini EA (60 WoSCC/67 PubMed papers), Waldinger MD (59 WoSCC/90 PubMed papers), and Rowland DL (51 WoSCC/68 PubMed papers), contributing significantly to the literature. The United States was the major contributor, with 384 papers. Keyword cluster analysis revealed that research focuses on PE diagnosis, pharmaceutical treatment (e.g., selective serotonin reuptake inhibitors [SSRIs] and antidepressants), etiology (e.g., serotonin [5-HT], NOS, and vas deferens), and relationships with prostate, thyroid, anxiety, depression, and other risk factors.</p>\u0000 <p><b>Conclusion:</b> Based on the knowledge map, this study provides an intuitive representation of the recent 20 years of PE research, offering a preliminary analysis of major research teams and exploring research hotspots, frontiers, and trends. This analysis aims to guide researchers and serve as a reference for future research on PE.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9984316","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vim3 and Mxi-2 as Promising New Molecular Biomarkers for the Diagnosis of Sperm Disorders","authors":"Richard Weiten,&nbsp;Jan Herden,&nbsp;Manuel Huerta,&nbsp;Tim Nestler,&nbsp;Johan Denil,&nbsp;Axel Heidenreich,&nbsp;Enno Storz,&nbsp;Melanie von Brandenstein","doi":"10.1155/and/1233135","DOIUrl":"https://doi.org/10.1155/and/1233135","url":null,"abstract":"<div>\u0000 <p><b>Introduction:</b> Male infertility remains a significant clinical challenge that requires the exploration of new diagnostics strategies. Truncated proteins, such as Vim3, a truncated variant of vimentin, show promise as a new class of biomarkers for diagnosing fertility disorders. Our objective was to assess the potential utility of Vim3 and Mxi-2, a shortened iteration of mitogen-activated protein kinase (MAPK) p38, as noninvasive biomarkers for diagnosing sperm disorders.</p>\u0000 <p><b>Methods:</b> Semen samples of 19 fertile males (control group) and 82 infertile males with semen abnormalities and no female factor (<i>n</i> = 28 OAT [oligo–astheno–teratozoospermia] syndrome, <i>n</i> = 8 azoospermia, <i>n</i> = 29 teratozoospermia, <i>n</i> = 11 astheno–teratozoospermia, and <i>n</i> = 6 oligozoospermia) were analyzed using ELISA (enzyme-linked immunosorbent assay) and flow cytometry. Furthermore, we conducted a lateral flow assay (LFA) specific to Vim3.</p>\u0000 <p><b>Results:</b> Our analysis revealed that Vim3 and endothelin-1 (ET-1) expression levels were significantly downregulated in non-normozoospermic patients (e.g., OAT syndrome), while Mxi-2 was overexpressed in these patients compared to the control group (<i>p</i> &lt; 0.01). Additionally, we introduced a novel LFA that provides a user-friendly and effective method for examining Vim3 as a biomarker in clinical settings.</p>\u0000 <p><b>Conclusion:</b> Our results suggest that Vim3 and Mxi-2 have potential as noninvasive semen biomarkers for diagnosing sperm disorders.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1233135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}