Andrologia最新文献

{"title":"Combined Transrectal Ultrasound Five-Grade Scoring System and Multiparametric MRI PI-RADS Score for Risk Prediction Modeling of Prostate Cancer and Clinically Significant Prostate Cancer in the PSA Gray Zone","authors":"Qi Dong,&nbsp;Zhen Tong,&nbsp;Jianguo Xia,&nbsp;Wenqi Yang,&nbsp;Guiqin Liu,&nbsp;Guangyu Wu,&nbsp;Shaowei Xie,&nbsp;Hongli Li","doi":"10.1155/2024/6699676","DOIUrl":"https://doi.org/10.1155/2024/6699676","url":null,"abstract":"<div>\u0000 <p><b>Purpose:</b> This study aims to develop and validate predictive nomograms for prostate cancer (PCa) and clinically significant PCa (csPCa) in prostate-specific antigen (PSA) gray zone of 4−10 ng/mL, utilizing transrectal ultrasound five-grade scoring system (TRUS-5), prostate imaging reporting and data system (PI-RADS) score based on multiparametric magnetic resonance imaging (mpMRI) and clinical parameters.</p>\u0000 <p><b>Method:</b> A retrospective analysis was conducted on a cohort of 383 patients with elevated PSA levels in the gray zone who underwent initial TRUS-guided 12-core prostate biopsy between January 2018 and December 2020. Multivariate logistic regression analyses were utilized to identify independent predictors for PCa and csPCa and to establish predictive nomograms. The diagnostic performance, calibration ability, and clinical usefulness of each model were assessed through receiver operating characteristic (ROC) curve analysis, calibration curve analysis, and decision curve analysis (DCA), respectively.</p>\u0000 <p><b>Results:</b> Two diagnostic nomograms were developed based on five risk factors (age, free/total PSA [(f/t) PSA] ratio, prostate volume [PV], TRUS-5, and PI-RADS score) to predict PCa and five risk factors (PSA, (f/t) PSA, PV, TRUS-5, and PI-RADS score) for csPCa of PSA gray zone patients. The TRUS-5/PI-RADS combined model demonstrated higher area under the curves (AUCs) (0.865 for PCa and 0.910 for csPCa) compared to the models based on PI-RADS score or TRUS-5 (<i>p</i>  &lt; 0.05), as well as the greater net benefits across a wide range of threshold probabilities for PSA gray zone patients.</p>\u0000 <p><b>Conclusion:</b> The current study presented valuable TRUS-5/PI-RADS combined nomograms for predicting PCa or csPCa in patients within the PSA gray zone, which may serve as useful tools for the clinical management of PCa and csPCa.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6699676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Beneficial Role of Low PVP Concentration on Sperm Apoptotic Gene Expression, Embryo Morphokinetics Status, and Clinical ICSI Outcomes","authors":"Arefeh Akbari Javar,&nbsp;Behrouz Aflatoonian,&nbsp;Ali Reza Talebi,&nbsp;Fatemeh Dehghanpour,&nbsp;Mohammad Ali Khalili,&nbsp;Fateme Montazeri","doi":"10.1155/2024/8803887","DOIUrl":"https://doi.org/10.1155/2024/8803887","url":null,"abstract":"<div>\u0000 <p>During the intracytoplasmic sperm injection (ICSI) procedure, 10% polyvinylpyrrolidone (PVP) is commonly used as a selection medium to decrease sperm motility. The study aimed to determine the effect of different concentrations of PVP (5% and 10%) on sperm apoptotic transcripts, sperm DNA fragmentation (SDF), embryo cytokinetic, and clinical characteristics in the ICSI program. In the study, 60 couples with male factor infertility underwent ICSI insemination using either 5% or 10% PVP concentration. Metaphase II (MII) oocytes were divided into two groups, with one group injected with sperm in 5% PVP and the other in 10% PVP. After fertilization, the zygotes were cultured using a time-lapse microscope to assess morphokinetic parameters also, simultaneously with the ICSI procedure, SDF and apoptotic transcript levels were quantified using reverse transcription quantitative polymerase chain reaction (qPCR). The study found that DNA fragmentation was lower in sperm from the 5% PVP group compared to the 10% PVP group. Additionally, the expression levels of B-cell lymphoma (BCL2) and the 70 kilodalton heat shock proteins (HSP70) genes were significantly higher in 5% PVP compared to 10% PVP, while the transcript levels of the Bcl-2-associated X protein (BAX) gene were lower in 5% PVP. The 5% PVP group also demonstrated higher fertilization and high-quality embryo formation rates. Cytokinetic variables were significantly different between the two groups, and the rates of fragmentation, uneven blastomere, reverse cleavage (RC), and embryo arrest were higher in the 10% PVP group. The study concluded that a low concentration of PVP (5%) is a suitable replacement for 10% PVP in ICSI cycles for male factor infertility.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8803887","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish Consumption Once a Week Improves Sperm Quality and Testosterone Levels","authors":"Bogdan Roussev,&nbsp;Todorka Sokrateva,&nbsp;Galina Nenkova,&nbsp;Ayshe Salim,&nbsp;Diana Ivanova","doi":"10.1155/2024/4680357","DOIUrl":"https://doi.org/10.1155/2024/4680357","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> The rate of male infertility has increased in the last decades. One factor that may improve sperm quality and testosterone is fish consumption, which supplies the organism with essential nutrients.</p>\u0000 <p><b>Aim:</b> To determine evidence of a relationship between the frequency of fish consumption and sperm quality.</p>\u0000 <p><b>Methods:</b> Seventy-four healthy men, aged 25–45 years, were involved in this observational study. Semen quality was evaluated by spermogram analyses and testosterone was measured in blood. Volunteers were classified as low, moderate, and high fish consumption groups according to the replies indicated in a questionnaire they had to fill out.</p>\u0000 <p><b>Results:</b> The moderate fish consumption group showed better semen quality with all spermogram parameters in the reference range and the low fish consumption group manifested the worst semen quality with sperm morphology below the reference range. The low fish consumption group had lower testosterone levels (3.6 ± 0.9 vs. 4.3 ± 1.5; <i>p</i> = 0.04), decreased nonprogressive spermatozoa (11.9 ± 6.2 vs. 15.8 ± 8.2; <i>p</i> = 0.04), and lower semen volume (3.1 ± 2.0 vs. 3.6 ± 1.3; <i>p</i> = 0.06) compared to the moderate fish consumption group. Positive associations were detected between the frequency of fish consumption, the semen volume, and nonprogressive spermatozoa, respectively.</p>\u0000 <p><b>Conclusions:</b> Fish consumption at moderate level such as once a week leads to improvement in sperm quality and testosterone levels.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4680357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Efficacy and Safety of Low-Intensity Pulsed Ultrasound Therapy for the Treatment of Peyronie’s Disease (A Report of 106 Cases): A Retrospective Study","authors":"Lei Yu,&nbsp;Yu Pan,&nbsp;Hongqiang Wang,&nbsp;Xiaojing Guo,&nbsp;Ling Fu,&nbsp;Peitao Wang,&nbsp;Yaowu Gao,&nbsp;Shaoping Jing,&nbsp;Shenqian Li,&nbsp;Kaishu Zhang,&nbsp;Qiang Li,&nbsp;Hanshu Wang,&nbsp;Tao Jing","doi":"10.1155/2024/7920485","DOIUrl":"https://doi.org/10.1155/2024/7920485","url":null,"abstract":"<div>\u0000 <p><b>Objective:</b> To evaluate the efficacy and safety of low‑intensity pulsed ultrasound (LIPUS) for the treatment of Peyronie’s disease (PD).</p>\u0000 <p><b>Methods:</b> To analyze the treatment effect of 106 PD patients, 56 patients treated with LIPUS as therapy group and 50 patients with oral L-carnitine and vitamin E as control group. 56 patients were treated with LIPUS three times per week for four consecutive weeks (12 times totally). Penile erectile pain, erectile function, the size of penile plaques, and penile curvature were assessed for all of these 106 PD patients before treatment and at 4- and 24-week follow-ups to evaluate the treatment effect. Erectile pain was assessed by visual analog scale (VAS), while erectile function using the International Index of Erectile Function scale (IIEF-5), and penile plaque size was measured by ultrasound. After alprostadil was injected into the penile body to induce a full erection, the penis was measured with a protractor at the maximum bending degree.</p>\u0000 <p><b>Results:</b> After 4-weeks follow-up, the VAS scores of the LIPUS group were significantly lower than the baseline (<i>P</i> &lt; 0.01), while no significant differences were found in the control group (<i>P</i> &gt; 0.05). After 24-weeks follow-up, the VAS scores of the control group reduced significantly but also were significantly higher than the LIPUS group (<i>P</i> &lt; 0.01). The IIEF-5 scores of the LIPUS group were significantly higher than the baseline (<i>P</i> &lt; 0.01). While the IIEF-5 scores of the control group showed no significant differences both after 4- and 24-weeks follow-up (<i>P</i> &gt; 0.05). The penile plaque size showed no significant differences after 4- and 24-weeks follow-up both in the LIPUS group and the control group (<i>P</i> &gt; 0.05). The penile curvature degrees showed a significant reduction after 4-weeks follow-up in LIPUS group (<i>P</i> &lt; 0.01). While the penile curvature degrees of the control group showed no significant differences both after 4- and 24-weeks follow-up (<i>P</i> &gt; 0.05). No complications, such as penile pain, hematoma, or skin ecchymosis, were observed during the entire process of LIPUS treatment, nor did they appear during follow-up.</p>\u0000 <p><b>Conclusions:</b> In patients with acute PD, LIPUS could relieve erectile pain and improve erectile dysfunction. Moreover, it appears not to reduce plaque size and not to have a clinically significant effect on penile curvature. Furthermore, LIPUS also shows a good safety profile during the management of PD and should be recommended for the clinical management of PD.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7920485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Native and Liposomal Curcumin Against Acute Reproductive Toxicity Induced by Cadmium Chloride in Male Mice","authors":"Arezou Azizi,&nbsp;Mohaddeseh Mohammadi-Sardoo,&nbsp;Fariba Sharififar,&nbsp;Majid Zeinali,&nbsp;Abbas Pardakhty,&nbsp;Maryam Iranpour,&nbsp;Elaheh Mohammadi,&nbsp;Mahshid Moballegh Nasery,&nbsp;Ali Mandegary","doi":"10.1155/2024/6658407","DOIUrl":"https://doi.org/10.1155/2024/6658407","url":null,"abstract":"<div>\u0000 <p>Exposure to cadmium chloride (Cd) is linked to reproductive disorders. Curcumin (CUR), a traditional medicinal compound, has been considered for its therapeutic properties. The present study evaluated the antioxidant effects of CUR and liposomal CUR on Cd-induced testicular toxicity. Mice were exposed to Cd (2.5 mg/kg as a single intraperitoneal dose), Cd + CUR at 50, 100, and 200 mg/kg, and Cd + liposomal CUR (15 mg/kg) orally for 7 days. At the end of the treatment period, histological studies, sperm parameters, oxidative stress parameters, and blood–testis barrier (BTB) integrity were performed. Cd-induced histopathological deleterious changes in the testis and epididymis tissues increased BTB permeability. It caused a significant decrease in sperm count and viability (<i>p</i> &lt; 0.001), as well as an increase in sperm abnormality (<i>p</i> &lt; 0.001) and a reduction in the antioxidant activity of superoxide dismutase (SOD), glutathione (GSH) (<i>p</i> &lt; 0.001), and increased lipid peroxidation (LPO; <i>p</i> &lt; 0.001) compared to the control group. Co-treatment of CUR with Cd dose-dependently and significantly attenuated Cd’s destructive effects on sperm and oxidative stress parameters at 50 mg/kg (<i>p</i> &lt; 0.01) and 100, 200 mg/kg (<i>p</i> &lt; 0.001). Although the liposomal form of CUR showed protective effects against some Cd-induced changes, such as sperm viability and count (<i>p</i> &lt; 0.001), it did not significantly improve LPO and GSH levels. The results indicated that native CUR prevented Cd-induced reproductive toxicity in male mice by suppressing oxidative injury, and there was no superior effect of liposomal CUR over native CUR.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6658407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D Deficiency Affects the Sex Hormones and Testicular Function in Mice","authors":"Sina Abasi,&nbsp;Hengameh Safari,&nbsp;Mehdi Hajian,&nbsp;Fatemeh Dehghan Shahreza,&nbsp;Nima Tanhaevash,&nbsp;Mazdak Razi,&nbsp;Joël R. Drevet,&nbsp;Mohammad Hossein Nasr-Esfahani","doi":"10.1155/2024/6912179","DOIUrl":"https://doi.org/10.1155/2024/6912179","url":null,"abstract":"<div>\u0000 <p>This study aims to evaluate the genomic effects of vitamin D (VitD) on steroidogenesis, sperm structure, and function in mice models with artificially induced vitamin D deficiency (VDD), while maintaining calcium and phosphorus homeostasis. qRT-PCR was utilized to measure the steady-state RNA levels of key steroidogenic genes, including CYP11A1, CYP19A1, 17<i>β</i>HSDH1, 3<i>β</i>HSDV1, and CYP24A1, involved in VitD metabolism, in testicular extracts from mouse models with varying degrees of VDD severity. Estradiol, testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels were also assessed. Furthermore, testicular histomorphometric analysis and evaluation of sperm structural and functional parameters were conducted. The study reveals a linear decrease in the expression of monitored steroidogenic genes with increasing severity of VDD. Moreover, in cases of mild and moderate VDD, testicular extracts exhibited significantly reduced levels of LH, estradiol, and testosterone. VDD has substantial effects on testicular steroidogenesis, which directly impacts spermatogenesis, independent of its established role in calcium regulation. Consequently, the reduction in steroidogenesis due to VDD contributes to the diminished efficiency of testicular spermatogenesis in individuals experiencing VDD, a prevalent condition worldwide.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6912179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Biallelic Mutation c.131G>T in MEI1 Causes Meiotic Arrest and Nonobstructive Azoospermia","authors":"Xiang Huang,&nbsp;Chen Zhang,&nbsp;Congqiao Zhang,&nbsp;Weiyang Zhang,&nbsp;Zhiyuan An,&nbsp;Qiang Li,&nbsp;Junting Jia,&nbsp;Laifeng Ren","doi":"10.1155/2024/6683212","DOIUrl":"https://doi.org/10.1155/2024/6683212","url":null,"abstract":"<div>\u0000 <p>Nonobstructive azoospermia (NOA) is considered to be the most severe form of male infertility. Meiotic arrest is one of the major causes of NOA and can be caused by single gene mutations. Here, a novel homozygous missense mutation (c.131G&gt;T) in the MEI1 gene was identified through whole exome sequencing in a Chinese men with NOA caused by meiotic arrest. Bioinformatics and genetic analysis predicted that this novel mutation may be deleterious. Our findings confirm that spermatogenesis is arrested at the primary spermatocyte stage, with defective programed DNA double-strand breaks (DSBs) formation. In vitro functional analysis revealed that the defects of DSBs formation may be a result of a lower expression level of MEI1 protein in the mutant. Therefore, the NOA in this patient is likely caused by the single novel mutation in the MEI1 gene leading to meiotic arrest. Our report expands the mutation spectrum of MEI1 and provides new evidence that the MEI1 gene may serve as a diagnostic marker for genetic counseling in NOA patients.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6683212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142007225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HIF-1α Affects Sperm Autophagy and Motility","authors":"Linfeng Mo,&nbsp;Jiwei Wu,&nbsp;Liangzhao Liu,&nbsp;Han Liu,&nbsp;Chaoyan Ou,&nbsp;Yonghua He","doi":"10.1155/2024/4575305","DOIUrl":"https://doi.org/10.1155/2024/4575305","url":null,"abstract":"<div>\u0000 <p>Male infertility is frequently caused by decreased sperm motility. Sperm of low progressive motility showed abnormal energy metabolism and elevated autophagy rate, although the specific mechanisms remain elusive. Hypoxia-inducible factor 1<i>α</i> (HIF-1<i>α</i>) is a transcription factor activated by hypoxia and plays a crucial role in regulating biological oxygen homeostasis. Negative correlation between sperm motility and HIF-1<i>α</i> expression and a potential association with the PI3K/Akt/mTOR signaling pathway were observed. To further explore the mechanisms by which HIF-1<i>α</i> impacts sperm motility, normal human sperm were treated with cobalt chloride (CoCl₂) or YC-1. Sperm autophagy rate, ATP production, levels of intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and HIF-1<i>α</i>, p-AKT, p-mTOR, and LC3B were assessed. The results showed that CoCl₂ or YC-1 decreased sperm motility and increased sperm autophagy rate. The levels of intracellular ROS, HIF-1<i>α</i>, p-AKT, p-mTOR, and LC3B were significantly increased, while the levels of sperm ATP and MMP reduced. These findings suggested that HIF-1<i>α</i> might affect mitochondrial structure and energy generation, leading to increased autophagy rate and decreased motility in human sperm, with the PI3K/Akt/mTOR pathway potentially playing a role in this process by exerting an inhibitory effect on autophagy.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4575305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141584106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complanatuside A Inhibits Seminal Vesicle Cell Apoptosis by Activating SerpinB1a in Type 2 Diabetic Mice","authors":"Dawei Ni,&nbsp;Kun Liu,&nbsp;Bin You,&nbsp;Ning Wu,&nbsp;Wei Wu,&nbsp;Yanbin Zhang,&nbsp;Baibing Yang,&nbsp;Yutian Dai","doi":"10.1155/2024/2994194","DOIUrl":"https://doi.org/10.1155/2024/2994194","url":null,"abstract":"<div>\u0000 <p>Type 2 diabetes mellitus (T2DM) can trigger cell apoptosis of seminal vesicles (SVs) and impair seminal secretory functions. Complanatuside A (CA) is known for its anti-inflammatory and antioxidant effects, as well as its ability to repair cellular damage. This study aimed to explore the potential molecular mechanisms through which CA mitigates cell apoptosis in the SVs of type 2 diabetic mice. A streptozotocin-induced type 2 diabetic mice model was utilized, and a 1-month intervention using CA (70 mg/kg) was administered. We monitored body weight, blood glucose levels, SV volume, and concentration of SV fluid. Hematoxylin and eosin staining was used to assess tissue damage. RNA sequencing was applied to identify differential gene expression, and the expression of target genes (ARG2, PBK, SerpinB1a, E2F2) was verified by qRT-PCR. Changes in the apoptosis level of SV tissues in mice were detected with TUNEL staining. Compared to the control group, mice with T2DM exhibited decreased SV volume and SV fluid content, which was improved with CA treatment. Elevated expression of SerpinB1a and reduced apoptosis were observed in the T2DM-CA group compared to T2DM mice. In summary, CA can inhibit cell apoptosis in the SVs, there by improving tissue damage in type 2 diabetic mice. SerpinB1a may be involved in this process. This study provides a new theoretical foundation for the treatment of seminal vesicle secretory dysfunction in type 2 diabetes mellitus.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/2994194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141584034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of miR-486 Expression in the Corpus Cavernosum of Spontaneously Hypertensive Rats Improves Erectile Function","authors":"XiangJun Kong,&nbsp;Jing Liu,&nbsp;QinWen Liu,&nbsp;Ge Li,&nbsp;Yong Cheng,&nbsp;Rui Jiang","doi":"10.1155/2024/8843591","DOIUrl":"https://doi.org/10.1155/2024/8843591","url":null,"abstract":"<div>\u0000 <p>This study aims to investigate whether transfection of miR-486 in the corpus cavernosum of spontaneously hypertensive rats (SHR) can improve erectile function and its mechanism. SHR penile cavernous smooth muscle cells were divided into control group, mimic negative control transfection group, inhibitor negative control transfection group, miR-486 mimic transfection group, and miR-486 inhibitor transfection group. miR-486 mimic (50 nM), miR-486 inhibitor (100 nM), and corresponding negative control preparation were transfected into the corresponding groups of cells. The mRNA expressions of miR-486, TGF-<i>β</i>1, Collagen I, and Collagen III in each group were detected after transfection. Twelve 12-week-old healthy male SHR and 12 WKY rats were randomly divided into WKY control group, SHR control group, SHR + Agomir negative control (NC) group, and SHR + miR-486 Agomir group. In the SHR + miR-486 Agomir group, penile cavernosa was transfected with miR-486 agonist. Penile sponge internal pressure (ICPmax)/mean arterial pressure (MAP), the expression of miR-486, the ratio of smooth muscle to collagen (SM/C), and the expression of TGF-<i>β</i>1, Collagen I, and Collagen III in penile cavernosum tissues were determined. Compared with the control group, the expressions of TGF-<i>β</i>1, Collagen I, and Collagen Ⅲ in the miR-486 mimic transfection group of SHR rat corpus cavernosum smooth muscle cells were significantly decreased, while those in the miR-486 inhibitor transfection group were significantly increased (<i>P</i>  &lt; 0.05). The ratios of ICPmax/MAP and SM/C in the SHR group were significantly lower than those in the WKY group and in the SHR + miR-486 Agomir group were significantly higher than those in the SHR group and the SHR + Agomir NC group (<i>P</i>  &lt; 0.05). The expressions of TGF-<i>β</i>1, Collagen I, and Collagen Ⅲ in SHR group were significantly increased compared with those in WKY group, while those in SHR + miR-486 Agomir group were significantly lower than those in SHR group and SHR + Agomir NC group (<i>P</i>  &lt; 0.05). The upregulation of the expression of miR-486 in the corpus cavernosum of SHR rats can inhibit fibrosis of the corpus cavernosum and improve erectile function.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8843591","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141439571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}