Andrologia最新文献

{"title":"Development of a Multivariable Prediction Model for Benign Prostatic Hyperplasia (BPH) in Chinese Middle-Aged and Elderly Patients","authors":"Jie Zhang,&nbsp;Li Zhang,&nbsp;Guobing Lin,&nbsp;Fan Zhao,&nbsp;Miaoyong Ye","doi":"10.1155/and/8013097","DOIUrl":"https://doi.org/10.1155/and/8013097","url":null,"abstract":"<p>This study aimed to develop and validate a multivariable prediction model for benign prostatic hyperplasia (BPH) among Chinese middle-aged and elderly men using pooled cross-sectional data from the 2011, 2013, and 2015 waves of the China Health and Retirement Longitudinal Study (CHARLS). A total of 8637 male participants aged ≥45 years were included and randomly divided into training (<i>n</i> = 6048) and validation (<i>n</i> = 2589) cohorts. Through univariate and multivariate logistic regression analyses, 12 independent predictors were identified: Frailty index (FI35), age, education level, urban/rural residence, smoking status, body mass index (BMI), sleep duration, instrumental activities of daily living (IADL), hypertension, diabetes, geographic region (North/South), and nap duration. A nomogram was constructed and demonstrated moderate predictive performance, with area under the curve (AUC) values of 0.709 (95% CI: 0.690–0.727) in the training cohort and 0.702 (95% CI: 0.673–0.731) in the validation cohort. The model exhibited good calibration and clinical utility, offering a practical tool for early BPH risk assessment in community-based primary care settings.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8013097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148615571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caffeine and Semen Quality: Safety and Mechanisms of Actions","authors":"Saleem A. Banihani","doi":"10.1155/and/3319657","DOIUrl":"https://doi.org/10.1155/and/3319657","url":null,"abstract":"<p>At present, coffee and tea have become the most consumed beverages worldwide. A defining characteristic of these drinks is their significant caffeine content, which is a methylxanthine alkaloid recognized for its central nervous system stimulant properties. Therefore, the impact of caffeine on human health remains an evolving area of research. One important route in this dynamic research is the impact of caffeine on male fertility. Even though, according to the PubMed database, over 500 articles have been published linking caffeine to sperm, the biochemical mechanisms through which caffeine affects semen quality are still complex and did not receive sufficient analyses. In this review, we aimed to map the current mechanisms based on the available published literature. In addition, we sought to summarize the evidence regarding the safety of caffeine consumption on human semen quality. To achieve this, we searched all published articles using the keywords “caffeine” combined with “sperm” and “semen.” A focused search was also performed using these terms along with “human” to filter for studies conducted specifically on human subjects. In conclusion, caffeine has been reported to exert positive effects on semen quality. Known mechanisms by which caffeine affects semen quality are via inhibiting phosphodiesterase (PDE) activity and increasing levels of cyclic adenosine monophosphate (cAMP), enhancing seminal creatine kinase activity, modulating sperm capacitation, inducing sperm hyperactivation independent of protein kinase A (PKA) pathway, reducing oxidative stress, and influencing steroidogenic enzyme activities. However, caffeine may induce potential toxicity to sperm when used at high doses.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/3319657","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148496767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D3 Protects Ram Epididymal Sperm From Oxidative Stress and Improves 4°C Storage Quality","authors":"Mohsen Shayestehyekta,&nbsp;Tayebeh Mohammadi,&nbsp;Leila Soltani,&nbsp;Mehrdad Pooyanmehr","doi":"10.1155/and/8230638","DOIUrl":"https://doi.org/10.1155/and/8230638","url":null,"abstract":"<p>Liquid storage of sperm increases oxidative stress (OS), reducing sperm quality. While vitamin D₃ (VD) has shown antioxidant and reproductive benefits in some species, limited studies have examined its effects on ram epididymal sperm, which are free from seminal plasma influences. This study explored how different doses of VD affect the epididymal sperm of Sanjabi rams under both normal and OS conditions and also during liquid storage at 4°C. Two experiments were conducted. The first tested three concentrations of VD (1.25, 6.25, and 31.25 µg/mL) under normal and OS conditions, analyzing antioxidant status by measuring total antioxidant capacity (TAC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) levels. The second experiment involved adding the same VD concentrations to extenders used for storing sperm at 4°C, with evaluations of sperm motility, viability, membrane integrity, DNA integrity, and morphological defects at 24, 48, and 72 h. VD inclusion (particularly at 1.25 and 6.25 µg/mL) was associated with increased TAC and SOD activities (<i>p</i> &lt; 0.05). These concentrations also reduced MDA levels (<i>p</i> &lt; 0.05), reflecting decreased OS. During liquid storage, samples treated with VD demonstrated improved sperm motility, viability, and membrane integrity (<i>p</i> &lt; 0.05). The concentration of 1.25 μg/mL consistently yielded the most favorable results, while 31.25 μg/mL caused increased morphological abnormalities and diminished DNA integrity over time. Adding low doses of VD to the extender enhanced sperm motility, viability, membrane stability, and DNA integrity during liquid storage. These findings suggest that VD, at the most effective concentrations within the tested range, may serve as a useful additive in ram sperm extenders for improving sperm preservation during liquid storage.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8230638","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148496772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Mespilus germanica Leaf Extract and Vitamin C Against Testicular Ischemia/Reperfusion-Induced Oxidative Stress and DNA Damage in Rats","authors":"Mehrdad Jafari,&nbsp;Mahdieh Raeeszadeh,&nbsp;Pejman Nazem Zomorodi,&nbsp;Loghman Akradi","doi":"10.1155/and/6962205","DOIUrl":"https://doi.org/10.1155/and/6962205","url":null,"abstract":"<p>Testicular ischemia/reperfusion (I/R) injury is a major contributor to male infertility, primarily through oxidative stress–mediated damage to lipids, DNA, and spermatogenic cells. This study aimed to evaluate the protective effects of <i>Mespilus germanica</i> (MG, medlar) leaf extract and vitamin C (VC), administered individually and in combination, on oxidative stress, DNA damage, and histopathological alterations in a rat model of testicular I/R injury. Thirty adult male Wistar rats were randomly assigned to five groups: negative control (C, no I/R) + saline, I/R + saline, I/R + medlar extract, I/R + VC, and I/R + MG + VC. Treatments (200 mg/kg, p.o.) were administered for 14 days before ischemia and reperfusion. Twenty-four hours postreperfusion, testes were analyzed for antioxidant enzyme activities (SOD, CAT), lipid peroxidation (MDA), DNA fragmentation, sperm parameters, and histopathological changes. Gas chromatography–mass spectrometry (GC–MS) analysis identified thymol (55.85%) and octadecane (11.36%) as the major constituents of the medlar extract. I/R significantly reduced body weight, antioxidant enzyme activities, and spermatogenesis, while increasing MDA levels, DNA fragmentation, and histological damage (<i>p</i> &lt; 0.01). Treatment with MG + VC markedly restored antioxidant defenses, preserved testicular architecture, and improved sperm quality, demonstrating a synergistic protective effect compared with individual treatments. Thymol-rich medlar leaf extract, particularly in combination with VC, exhibits potent protective effects against oxidative stress-induced testicular injury, suggesting its potential as a therapeutic strategy for mitigating reproductive toxicity.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6962205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148496798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of Advanced Sperm Selection Techniques in Assisted Reproductive Program: A Systematic Review","authors":"Mostafa Yousefi,&nbsp;Esmat Mangoli,&nbsp;Amin Salehi-Abargouei,&nbsp;Sahar Khosravi,&nbsp;Mohammad Ali Khalili","doi":"10.1155/and/4372382","DOIUrl":"https://doi.org/10.1155/and/4372382","url":null,"abstract":"<p>The quality of the spermatozoa likely influences embryo development and subsequent pregnancy outcomes. Advanced sperm selection techniques choose viable, mature, and structurally intact sperm with high DNA integrity for fertilization. This review study aimed to determine whether there is evidence on the overall benefit of these techniques on the morphokinetic parameters of embryos and clinical outcomes in ICSI. The protocol was registered with PROSPERO, and comprehensive searches were carried out in the Cochrane, PubMed, Google Scholar, and Scopus databases. After removal of duplicates, 35 articles underwent title and abstract screening, of which 16 were excluded. Full-text assessment was performed on the remaining studies, resulting in the inclusion of seven randomized controlled trials for qualitative synthesis. Most of the included studies were assessed as having a low risk of bias. Studies have reported higher fertilization, improved embryo morphology scores, and increased clinical pregnancy rates using advanced selection techniques compared to density gradient centrifugation or swim-up methods. While improvements in intermediate outcomes are consistently reported, the evidence for improved live birth rates remains less robust, with some studies failing to reach statistical significance. This underscores the need for larger, randomized clinical trials to validate the clinical utility of these techniques and establish standardized protocols for their use.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4372382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frozen Z. mioga Roscoe Flower Buds (ZM-FB) Alters the Testicular Immune Microenvironment and Spermatogenesis by Disturbing the Androgen Synthesis Pathway in Mice Testes","authors":"Rui-na Zhang,&nbsp;Jing Chen,&nbsp;Dao-lun Yu,&nbsp;Ren-shu Huang,&nbsp;Yong-ping Cai,&nbsp;Nai-fu Chen","doi":"10.1155/and/2046902","DOIUrl":"https://doi.org/10.1155/and/2046902","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Androgen from the testis is critical for maintaining immune environmental homeostasis and male spermatogenesis. <i>Z. mioga</i> Roscoe flower buds (ZM-FB) are edible plants belonging to the ginger family and popular among consumers for their high nutritional and medicinal value. However, the antiandrogen effect of ZM-FB and, subsequently, the reproductive outcome has not been evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This study aimed to explore the effect of ZM-FB on the reproductive health of male mice and its potential mechanism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The body weight, sperm concentration, testosterone level in serum, abundance of androgen synthesis-related genes, and testicular transcriptome were detected in mice from the control and experimental groups, which were fed 125 or 250 mg/kg fresh or frozen ZM-FB powder daily for 3–5 weeks.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared with the control group, mice fed 125 or 250 mg/kg fresh ZM-FB for 5 weeks exhibited no significant alterations in reproductive and immune indices. In contrast, administration of 250 mg/kg frozen ZM-FB powder for 3 weeks significantly reduced the testis/body weight ratio and sperm concentration and markedly altered the testicular transcriptomic profile in mice. Additionally, RNA-Seq analysis identified 480 differentially expressed genes (DEGs) in mouse testicular tissue, which were mainly enriched in pathways related to androgen synthesis and immune function. These DEGs included notably downregulated genes (<i>Scd1</i>, <i>Star</i>, cytochrome P450 family 11 subfamily A1 [<i>Cyp11a1</i>], <i>Hsd3b6</i>, <i>Cyp17a1</i>, <i>Hsd17b3</i>, luteinizing hormone receptor [<i>Lhcgr</i>]) and upregulated MHC class II marker genes. These transcriptomic findings were further validated by qRT-PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conlusion</h3>\u0000 \u0000 <p>ZM-FB is a typical medicinal and edible ingredient. Fresh ZM-FB has no impact on male animal spermatogenesis and androgen synthesis, while frozen ZM-FB exerts adverse effects on spermatogenesis. This study provides reliable theoretical guidance for the rational dietary application of ZM-FB.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/2046902","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Unhealthy Lifestyle and Semen Quality in Chinese Sperm Donation Volunteers","authors":"Yi Lin,&nbsp;Dan Li,&nbsp;Xiaolin Zhong,&nbsp;Xinyi Deng,&nbsp;Ruijun Xu,&nbsp;Sirong Wang,&nbsp;Yuxin Bi,&nbsp;Lu Luo,&nbsp;Kaiwen Zuo,&nbsp;Jinying Huang,&nbsp;Ziquan Lv,&nbsp;Xinzong Zhang,&nbsp;Yuewei Liu,&nbsp;Qiling Wang","doi":"10.1155/and/7905935","DOIUrl":"https://doi.org/10.1155/and/7905935","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>An unhealthy lifestyle has been linked to declined semen quality; however, most previous studies have focused only on a single lifestyle factor, and the combined effect of an unhealthy lifestyle on semen quality remains unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We conducted a longitudinal study with repeated semen measurements among 3785 sperm donation volunteers at the Human Sperm Bank of Guangdong Province from 2020 to 2023. An unhealthy lifestyle score was used to assess participants’ unhealthy lifestyle levels and comprised six indicators, including body mass index (BMI), cigarette smoking, alcohol consumption, physical activity, sleep quality, and diet diversity. A higher score indicated a more unhealthy lifestyle. Semen analysis was conducted according to the 5^th edition of the World Health Organization laboratory manual for the examination and processing of human semen. Linear mixed models and linear regression models were used to evaluate the association between lifestyle and semen quality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Each 1-point increase in the unhealthy lifestyle score was significantly associated with a reduction of 0.1 mL (95% CI: 0.04, 0.1; adjusted <i>p</i> = 0.003) in semen volume, 6.5 × 10⁶ (2.1, 10.9; adjusted <i>p</i> = 0.016) in total sperm number, 3.9 × 10⁶ (1.1, 6.8; adjusted <i>p</i> = 0.016) in total motile sperm number, and 3.8 × 10⁶ (1.0, 6.6; adjusted <i>p</i> = 0.016) in total progressive sperm number. Stratified analyses showed significantly poorer semen quality among individuals aged &lt;25 years and those with high perceived stress levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We provide evidence that an unhealthy lifestyle is associated with lower semen quality. Adopting a fully healthy lifestyle has positive implications for semen quality and male reproductive health.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7905935","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a DPY19L2 Mutation Linking Globozoospermia to Congenital Heart Defects in a Consanguineous Family: A Genetic Analysis","authors":"Hongjing Li,&nbsp;Jun He,&nbsp;Chaojun Chen,&nbsp;Zongjian Tan,&nbsp;Weiming Chen,&nbsp;Maobo Tian","doi":"10.1155/and/7945760","DOIUrl":"https://doi.org/10.1155/and/7945760","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Male infertility is a complex condition with significant genetic heterogeneity. Globozoospermia, a rare form of teratozoospermia characterized by round-headed sperm, is frequently associated with mutations in the <i>DPY19L2</i> gene. While the link between <i>DPY19L2</i> and infertility is established, the co-occurrence of male infertility and congenital heart defects (CHDs) in offspring within consanguineous families highlights the necessity of investigating compounded genetic risks and independent segregation of recessive traits.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We recruited a consanguineous family comprising a male proband with infertility and a history of adverse reproductive outcomes, including an offspring with transposition of the great arteries (TGA). Semen analysis was performed according to WHO guidelines (2010, 5th Edition). Genetic investigation included karyotyping and whole-exome sequencing (WES) for the proband, his spouse, and family members. Sanger sequencing was used to validate identified variants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Semen analysis of the proband revealed 100% round-headed sperm (globozoospermia) with normal volume and density but reduced motility. WES identified a homozygous mutation in the <i>DPY19L2</i> gene (c.63dupG; p.Arg22Alafs<i> </i>^∗66). This mutation was also confirmed in the proband’s affected brother. Pedigree analysis suggested an autosomal recessive inheritance pattern resulting from consanguinity. The specific mutation explains the fertilization failure in previous intracytoplasmic sperm injection (ICSI) cycles and highlights the necessity for artificial oocyte activation (AOA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study confirms the pathogenic role of the <i>DPY19L2</i> c.63dupG mutation in familial globozoospermia. Furthermore, it highlights the critical importance of comprehensive genetic screening in consanguineous couples presenting with infertility and congenital anomalies. Early genetic diagnosis can guide appropriate assisted reproductive strategies, such as ICSI with AOA, and inform risk assessment for future pregnancies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7945760","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Moringa oleifera Aqueous Leaf Extract on TM3 Leydig Cells Against Hydrogen Peroxide-Induced Oxidative Stress","authors":"Kutullo Mohlala,&nbsp;Ugochukwu Offor,&nbsp;Chinyerum Sylvia Opuwari","doi":"10.1155/and/3367019","DOIUrl":"https://doi.org/10.1155/and/3367019","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p><i>Moringa oleifera</i> (<i>M. oleifera</i>) is a medicinal plant rich in dietary antioxidants that may protect reproductive cells from oxidative stress-induced damage and male fertility problems. This study investigated <i>M. oleifera</i> aqueous leaf extracts (ALE) effects on TM3 Leydig cells exposed to hydrogen peroxide (H₂O₂).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p><i>M. oleifera</i> ALE and ascorbic acid (10−1000 µg/mL) were assessed for antioxidant activity. TM3 Leydig cells were exposed to various concentrations of <i>M. oleifera</i> ALE (0−1000 µg/mL) or α-tocopherol (50 µM) for 24 h, alongside 200 µM H₂O₂, with or without hCG (4 ng/mL). Cell viability, testosterone production, mitochondrial membrane potential (MMP), total protein and oxidative stress parameters (reactive oxygen species [ROS], superoxide dismutase [SOD], reduced glutathione [GSH], total antioxidant capacity [TAC] and lipid peroxidation [MDA]) were assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ascorbic acid demonstrated superior DPPH radical scavenging and FRAP activity compared to <i>M. oleifera</i> ALE at most concentrations (<i>p</i>  &lt; 0.0001). H₂O₂ (200 µM) exposure significantly reduced cell viability, MMP, SOD, GSH and TAC levels, while increasing ROS and MDA levels (<i>p</i>  &lt; 0.05). Treatment with <i>M. oleifera</i> ALE or α-tocopherol significantly increased cell viability, total protein, SOD activity and GSH levels while reducing ROS and MDA compared to H₂O₂ control (<i>p</i>  &lt; 0.05). However, testosterone levels remained unchanged (<i>p</i>  &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p><i>M. oleifera</i> ALE effectively protects the TM3 Leydig cells from H₂O₂-induced oxidative stress by restoring the redox balance, though unchanged testosterone levels suggest limitations in using TM3 Leydig cells for assessing testosterone synthesis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/3367019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Effect of Betaine on Apoptosis and Oxidative Stress in Methotrexate-Induced Testicular Damage in Mice”","authors":"","doi":"10.1155/and/9897140","DOIUrl":"https://doi.org/10.1155/and/9897140","url":null,"abstract":"<p>F. Rezaei-Tazangi, N. Varaa, Z. Kazemikia, A. Goodarzi, and L. Khorsandi. “Effect of Betaine on Apoptosis and Oxidative Stress in Methotrexate-Induced Testicular Damage in Mice” <i>Andrologia</i>, no. 2024 (2024). https://doi.org/10.1155/and/4126428.</p><p>In the article titled “Effect of Betaine on Apoptosis and Oxidative Stress in Methotrexate-Induced Testicular Damage in Mice,” there was an error in Section 2.1. <i>Experimental Design</i> related to the number of mice used in the study.</p><p>The error is shown and corrected below:</p><p>[“40 healthy adult male Naval Medical Research Institute (NMRI) mice”]</p><p>Should read</p><p>[“48 healthy adult male Naval Medical Research Institute (NMRI) mice”]</p><p>We apologize for this error.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/9897140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148237871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}