Andrologia最新文献

{"title":"Naringenin Attenuates Bisphenol A-Induced Leydig Cell Damage by Activating Autophagy","authors":"Yousef Asadi-Fard,&nbsp;Layasadat Khorsandi,&nbsp;Elham Younesi,&nbsp;Mohammad Javad Khodayar,&nbsp;Abbass Heidari-Moghadam","doi":"10.1155/and/5537141","DOIUrl":"https://doi.org/10.1155/and/5537141","url":null,"abstract":"<p>The toxic effects of bisphenol A (BPA) on the male reproductive system have been shown in previous research. The impact of naringenin (NG) on BPA-induced Leydig cell (TM3 cell line) toxicity and its mechanism was examined in the present study. The TM3 cells were treated with BPA or NG in various groups for 48 h. To analyze the autophagy role of NG and BPA in TM3 cells, the cells were pretreated with 3-methyladenine (3-Ma), an autophagy inhibitor. The viability rate of TM3 cells notably decreased when exposed to the BPA group. BPA induces oxidative stress in TM3 cells by diminishing crucial antioxidant components such as glutathione, superoxide dismutase, catalase, and glutathione peroxidase while simultaneously increasing the generation of reactive oxygen species (ROS). BPA significantly increased the Bax/Bcl-2 ratio and ROS generation while decreasing testosterone concentration. The expression of autophagy-inducer genes (ATG7, ATG5, and Beclin-1) and the ratio of LCSII/LC3I proteins were significantly reduced, while mTOR expression was increased in the BPA group. NG pretreatment decreased the Bax/Bcl-2 ratio and expression of mTOR, while increasing the LC3II/LC3I ratio and the expression of ATG5, ATG7, and Beclin-1 proteins. NG could also reverse testosterone and antioxidant levels of the BPA-intoxicated cells. Treatment with 3-Ma led to a significant decrease in cell viability in TM3 cells upon exposure to NG and BPA, whereas the untreated 3-Ma groups showed higher viability. In the presence of 3-Ma, NG caused a decline in testosterone levels and antioxidant biomarkers while raising the Bax/Bcl-2 ratio and ROS production in TM3 cells exposed to BPA. These results propose that NGs protect TM3 cells from BPA by enhancing autophagy and lowering apoptosis and oxidative stress.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5537141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Wet Cupping With Sperm Quality and Mitochondrial Gene Expression in a Rat Model of Metabolic Male Infertility","authors":"Soha Albeitawi,&nbsp;Mariam Al-Omari,&nbsp;Fatimah Almahasneh,&nbsp;Nesreen Bataineh,&nbsp;Tareq Al-Omari","doi":"10.1155/and/4092625","DOIUrl":"https://doi.org/10.1155/and/4092625","url":null,"abstract":"<p>Infertility is an increasingly common condition associated with significant psychological and economic burdens. Treatments of infertility have limited success rates and come with a high cost and emotional toll, which highlights the need for other therapies. Wet cupping has been investigated for the treatment of different conditions, but not infertility. We therefore investigated the effects of wet cupping in a rat model of metabolic male infertility. Sperm concentration and viability, testicular weight, epididymal weight, Johnson score, and serum testosterone (T) significantly improved after wet cupping (<i>p</i>-value = 0.026, 0.026, 0.006, 0.015, and 0.004, respectively). In addition, wet cupping was associated with an increased expression of NDUF8 and SDHB mitochondrial genes (<i>p</i>-value = 0.041), and <i>TNF-α</i> (<i>p</i>-value = 0.035), while no significant effect was observed on sperm’s morphology and motility, and follicle-stimulating hormone (FSH) and luteinizing hormone (LH) serum levels. Our results suggest that wet cupping can have a potential role in treating male infertility. However, there are several limitations, such as serum inflammatory markers and oxidative stress markers were not measured. Therefore, further studies are required to investigate the related mechanism and to prove any beneficial effects.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4092625","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Racial Differences in Semen Quality: A Systematic Review and Meta-Analysis of Semen Parameters","authors":"Subin Park,&nbsp;Su-Yeon Cho,&nbsp;Yujin Kwon,&nbsp;Hyun Joo Lee,&nbsp;Eun Hee Yu,&nbsp;Jong Kil Joo,&nbsp;Ki-Tae Ha,&nbsp;Dokyoung Kim,&nbsp;Dongjun Lee,&nbsp;Jin-Woo Kim,&nbsp;Won Kyu Kim,&nbsp;Kihun Kim,&nbsp;Yun Hak Kim","doi":"10.1155/and/7310532","DOIUrl":"https://doi.org/10.1155/and/7310532","url":null,"abstract":"<p>Racial and ethnic differences in semen quality are crucial for understanding disparities in male reproductive health and infertility, yet these variations remain underexplored in the global context. We assess racial differences in semen quality, including key parameters, such as sperm concentration, motility, morphology, and total sperm count, while comparing outcomes between fertile and infertile groups, and examining variations across geographic regions and socioeconomic contexts, including comparisons between high-income and middle-income countries. A comprehensive search of PubMed, Embase, Scopus, and Web of Science was conducted for studies published from 1985 to 2024. Semen parameters included semen volume, sperm concentration, total sperm count, sperm motility, progressive motility, viability, and morphology. Data from 40 studies comprising 71,880 samples were synthesized using a random-effects meta-analysis, following PRISMA guidelines. Asians exhibited the highest sperm concentration (53.79 million/mL) and motility (54.97%), while Blacks showed the lowest values in sperm morphology (7.85%) and total sperm count (78.04 million). Fertile groups consistently outperformed infertile groups across all parameters, with significant declines in motility and morphology in the infertile population. ANOVA and Tukey post hoc tests confirmed significant intergroup differences. This study highlights substantial racial differences in semen quality, influenced by genetic, environmental, and socioeconomic factors. The findings emphasize the need for culturally tailored interventions and future research to address global disparities in male reproductive health.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7310532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidants and Male Infertility: Interpreting Meta-Analytical Evidence to the Skeptics and Discerning Clinicians","authors":"Selahittin Çayan,&nbsp;Rossella Cannarella,&nbsp;Mohamed Arafa,&nbsp;Ashok Agarwal","doi":"10.1155/and/4666847","DOIUrl":"https://doi.org/10.1155/and/4666847","url":null,"abstract":"<div>\u0000 <p>Elevated levels of oxidative stress (OS) in semen are linked to sperm dysfunction, DNA damage, and reduced male fertility, leading to impaired sperm parameters. Antioxidants (AOXs) are widely available, relatively inexpensive, and more accessible compared to other medical and surgical treatments for male infertility. However, there is significant debate in the literature regarding their efficacy, and a clear consensus has yet to be reached. This review aims to evaluate the use of AOX in male infertility by analyzing the latest meta-analytical evidence and presenting case scenarios. Our review found evidence-based consensus demonstrating significant improvements in pregnancy rates and sperm parameters following AOX treatment. However, a careful clinical workup and additional randomized controlled trials (RCTs) are necessary to support AOX treatment in infertile men.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4666847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-carnitine Ameliorates Disturbed Testicular Parameters and Sex Hormone Level in Movento-Exposed Mice: Mitigation of Oxidative Stress and Inflammation","authors":"Ali Shamsara,&nbsp;Golnaz Shafiei,&nbsp;Mohammad Amin Rajizadeh,&nbsp;Tahereh Haghpanah,&nbsp;Faezeh Akhgarandouz,&nbsp;Iman Zangiabadi","doi":"10.1155/and/5555588","DOIUrl":"https://doi.org/10.1155/and/5555588","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Spirotetramat (Movento) is one of the most widely used pesticides in agriculture. Prolonged exposure to Movento may adversely affect male fertility by inducing oxidative stress and inflammation. Conversely, L-carnitine (LC), a potent antioxidative and anti-inflammatory compound, has been shown to mitigate cellular damage. This study evaluates the protective effect of LC against Movento-induced testicular injury in male mice.</p>\u0000 <p><b>Methods:</b> Thirty-two male mice (9–10 weeks old and weighing 25–35 g) were divided into four groups: Control (Con), received saline (Movento vehicle) via gavage for 35 days. Movento (M), received Movento (1000 mg/kg) every 48 h for 35 days via gavage. LC + Movento (LC + M), treated with LC (200 mg/kg) after Movento administration (same as M group). LC-only (LC), received LC (200 mg/kg) for 35 days via gavage. After treatment, mice were euthanized, and testicular histology, sperm parameters, inflammatory cytokines, and sex hormone levels were assessed.</p>\u0000 <p><b>Results:</b> Movento exposure significantly increased sperm abnormalities, reduced sex hormone levels, and disrupted seminiferous tubule morphology compared to controls. However, LC consumption could partially improve the diminished sex hormone levels, sperm quality, and testicular structure, likely due to its antioxidative and anti-inflammatory properties.</p>\u0000 <p><b>Conclusion:</b> LC ameliorates Movento-induced testicular damage by attenuating oxidative stress and inflammation.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5555588","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Mitochondrial-Associated Proteins on Erectile Dysfunction: Insights From Mendelian Randomization Analysis","authors":"Yijia Fu,&nbsp;Xin Zhang,&nbsp;Jie Wang,&nbsp;Runnan Xu,&nbsp;Qi Zhang,&nbsp;Jianxiong Ma,&nbsp;Bodong Lv","doi":"10.1155/and/4615891","DOIUrl":"https://doi.org/10.1155/and/4615891","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Mitochondrial dysfunction is implicated in the pathogenesis of erectile dysfunction (ED); establishing a causal relationship remains a challenge. This study employed a two-sample Mendelian randomization (MR) approach to investigate the potential causal associations between mitochondria-associated proteins and ED.</p>\u0000 <p><b>Methods:</b> Association data on mitochondria-associated proteins from the IEU OpenGWAS database were used for exposure, whereas ED association data from the UK Biobank and FinnGen databases served as the outcome. MR analyses were conducted separately, primarily employing the inverse-variance weighted (IVW) method and supplemented by the MR-Egger, weighted median, simple mode, and weighted mode methods. Sensitivity analyses included Cochran’s <i>Q</i> test, MR-Egger test, leave-one-out analysis, and MR-PRESSO. A meta-analysis of both databases was conducted to enhance the credibility of the results.</p>\u0000 <p><b>Results:</b> Meta-analysis revealed a significant causal relationship between five mitochondria-related proteins and ED: 39S ribosomal protein L33 (RPL33; <i>p</i> = 0.013; odds ratio [OR] = 0.94; 95% confidence interval [CI]: 0.90–0.99), mitochondrial ubiquitin ligase activator of NFKB-1 (MULAN1; <i>p</i> = 0.039; OR = 1.08; 95% CI: 1.00–1.16), nucleoside diphosphate-linked moiety X motif -8 (NUDT8; <i>p</i> = 0.035; OR = 0.92; 95% CI: 0.84–0.99), pyruvate dehydrogenase (acetyl-transferring) kinase isozyme-1 (PDK1; <i>p</i> = 0.047; OR = 1.07; 95% CI: 1.00–1.14), and serine-tRNA ligase (SerRS; <i>p</i> = 0.005; OR = 1.18; 95% CI: 1.05–1.33). Sensitivity analyses revealed no abnormalities.</p>\u0000 <p><b>Conclusion:</b> RPL33 and NUDT8 exhibited potential protective effects against ED, whereas MULAN1, PDK1, and SerRS may increase the risk of developing ED. These findings offer new insights into the role of mitochondrial dysfunction in ED pathogenesis and may guide the development of future therapeutic strategies.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4615891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of miR-425-3p and Seminal Microbiota on Semen Parameters: Insights Into Male Infertility","authors":"Osamah Batiha,&nbsp;Raghad Abu-Diak,&nbsp;Qutaiba Ababneh,&nbsp;Mahmoud A. Alfaqih,&nbsp;Mothanna Nawafleh,&nbsp;Raghad Abu Al-Rub,&nbsp;Masood Abu-Halima","doi":"10.1155/and/6262703","DOIUrl":"https://doi.org/10.1155/and/6262703","url":null,"abstract":"<div>\u0000 <p>Male infertility is a multifaceted condition influenced by various factors, resulting in diverse phenotypic expressions. MicroRNAs (miRNAs), oxidative stress, and microbiota are potential contributors to compromised semen quality. This study explores the relationship between miR-425-3p, seminal microbiota, and oxidative stress in relation to impaired semen parameters. Semen samples were collected from 39 individuals with sperm motility &lt;20% and 31 individuals with sperm motility &gt;20%. Microbial cultures were performed to analyze the microbiota in the samples. DNA extraction from seminal cultured colonies, followed by PCR amplification targeting the 16S rRNA gene was conducted for sequencing, while RNA extraction from seminal plasma facilitated RT-qPCR analysis of miR-425-3p. Additionally, oxidative lipid degradation was evaluated using a thiobarbituric acid reactive species (TBARS) assay, comparing malondialdehyde (MDA) levels between samples with good and poor sperm motility. Our analysis revealed a diverse semen microbiota, predominantly consisting of Staphylococcus, Enterococcus, Bacillus, Micrococcus, and Neisseria. Less prevalent genera included <i>Streptococcus, Acinetobacter, Corynebacterium, Actinomyces, and Escherichia coli</i>. Higher bacterial loads were positively correlated with improved sperm motility, averaging 20,373 CFU/mL in the group with sperm motility &gt;20% compared to 1247 CFU/mL in group with sperm motility &lt;20% (<i>p</i> = 0.03). Conversely, miR-425-3p expression was significantly elevated in group with sperm motility &lt;20%, showing a fourfold increase (<i>p</i> = 0.034). Reduced miR-425-3p expression was observed in samples abundant in Bacillus, particularly when Neisseria was absent. MDA levels, a marker of oxidative stress, were significantly higher in the less motile group (9.46 ± 5.07 nmol/mL) compared to the motile group (5.25 ± 3.32 nmol/mL, <i>p</i> = 0.038). The analysis revealed distinct microbial profiles associated with varying sperm motility levels. These findings establish a connection between increased miR-425-3p expression, elevated seminal oxidative stress, and decreased sperm motility. Our data provide insights into the roles of seminal microbiota and microRNA in the pathogenesis of impaired semen quality.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6262703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are Uncertain Vasectomy Pathology Reports Suggestive of Procedural Failure? Long-Term Data From a Large Cohort","authors":"Max D. Sandler,&nbsp;Laura Angulo-Llanos,&nbsp;Rohan Dureja,&nbsp;Adriana Sandino,&nbsp;Faaris Khan,&nbsp;Veronica Junco,&nbsp;Dan V. Tran,&nbsp;Julio Yanes,&nbsp;Adam D. Williams,&nbsp;Thomas A. Masterson","doi":"10.1155/and/6054225","DOIUrl":"https://doi.org/10.1155/and/6054225","url":null,"abstract":"<div>\u0000 <p><b>Purpose:</b> During vasectomy, technical errors such as difficulty identifying and transecting the vas deferens may occur. Pathology reports indicating an incompletely transected vas may create uncertainty regarding procedural success. However, there is a paucity of large cohort studies investigating this correlation. To address this gap, we explored the relationship between abnormal pathology reports and vasectomy failure rates to determine whether such reports should impact clinical decision making.</p>\u0000 <p><b>Methods:</b> We retrospectively reviewed charts of men aged ≥18 who underwent vasectomy between 2004–2024. Abnormal pathology reports failed to identify complete vas deferens cross sections. Vasectomy failure was defined as presence of motile sperm or ≥100,000 nonmotile sperm on postvasectomy semen analysis (PVSA) ≥3 months.</p>\u0000 <p><b>Results:</b> Of 2446 vasectomies with pathology reports, 2399 (98.1%) reports were normal, 1330 returned for PVSA, and 55 were failures (4.1%). Conversely, 47 (1.9%) had an abnormal report. Of these, 31 patients returned for PVSA, 87.1% (<i>n</i> = 27) were successes with failure rate of 9.7% (<i>n</i> = 3), and one patient had sperm on PVSA &lt;3 months after vasectomy but was lost to follow-up. The three failures were secondary to errors in transecting the vas on one side. Pathology reports stated “benign nerve and fibrovascular tissue,” “artery,” and “no vas deferens lumen visualized.”</p>\u0000 <p><b>Conclusions:</b> Outcomes from a large cohort over two decades suggests although pathology provides early feedback, abnormal reports do not reliably predict vasectomy failure. Pathology should not independently guide clinical decision making without confirmatory semen analysis, which remains the marker of success. Future research could investigate outcomes of patients with abnormal pathology who did not follow up for semen analysis.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6054225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Handling of Human Ejaculate Affects Osmolality and Yield by Density Gradient Centrifugation and Possibly Also Sperm DNA Integrity","authors":"Emma Holmes,&nbsp;Petr Houska,&nbsp;Lars Björndahl,&nbsp;Ulrik Kvist","doi":"10.1155/and/4015844","DOIUrl":"https://doi.org/10.1155/and/4015844","url":null,"abstract":"<div>\u0000 <p>During routine handling in the assisted reproductive technology (ART) laboratory, the osmolality of the ejaculate increases, and live spermatozoa adapt to this increase. Preparation media are generally isotonic, having an osmolality of ~290 mOsm/kg. Therefore, when the ejaculate is placed on a density gradient, the spermatozoa are exposed to a hypotonic challenge. The aim of this study was to investigate whether osmotic challenges affect the yield of density gradient centrifugation (DGC), the DNA fragmentation index (%DFI), and the proportion of high DNA stainable sperm (%HDS). Furthermore, it was investigated if these effects could be counteracted by dilution of the ejaculate. Semen samples from healthy donors were collected and used after written consent. Four protocols of different experimental conditions were followed. These included storage at 37°C, storage at room temperature (RT), dilution after liquefaction, and ejaculation directly into a buffer. Pairwise comparisons of the studied aliquot and its own control were performed. Storage at 37°C led to an increase in osmolality to 352 mOsm/kg and halved the yield to 4.5%. Storage at RT led to an increase in osmolality to 384 mOsm/kg and halved the yield to 5.8%. The %DFI and %HDS did not change during these storage conditions. In undiluted samples, osmolality increased to 373 mOsm/kg and in diluted to 323 mOsm/kg. The %DFI and %HDS did not change. After ejaculation into a buffer, the osmolality increased only to 315 mOsm/kg after storage and the yield halved after storage to 12.9%. The %DFI was significantly lower after ejaculation into a buffer compared to dilution after liquefaction, 6% versus 11%, respectively. No difference was observed in %HDS. The challenges to sperm function induced by osmolality changes, and possibly also sperm DNA integrity, can be counteracted by collecting ejaculates directly into an isotonic buffer.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4015844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ameliorative Effects of Probiotics on the Sperm Quality and Testicular Structure After Ischemia/Reperfusion Injury Following Testicular Torsion/Detorsion","authors":"Zohresadat Akbari,&nbsp;Narges Karbalaei,&nbsp;Saied Karbalay-Doust,&nbsp;Seyed Shahram Shekarforoush,&nbsp;Farhad Koohpeyma,&nbsp;Sourena Rastin","doi":"10.1155/and/8508956","DOIUrl":"https://doi.org/10.1155/and/8508956","url":null,"abstract":"<div>\u0000 <p>Oxidative stress is a critical contributor to the pathophysiology of ischemia/reperfusion (I/R) injury associated with testicular torsion (TT) and detorsion (D) (TT/D). This study examines the antioxidative properties of the probiotics Lactobacillus (L.) reuteri and Lactobacillus (L.) rhamnosus in ameliorating TT/D-induced oxidative and histological damage. Male Sprague–Dawley rats (<i>n</i> = 6 per group) were allocated into five experimental groups: a sham-operated group, a torsion/detorsion (T/D) group, and three T/D groups treated with <i>L. reuteri</i> (T/D+LRe), <i>L. rhamnosus</i> (T/D+LRm), or a combination of both (T/D+LRe+LRm). TT was induced by rotating the right testis 720° clockwise for 2 h, followed by 60 days of reperfusion. The testis was then excised for further analysis. The evaluated parameters included sperm quality, hormone levels (FSH, LH, and testosterone), as well as levels of malondialdehyde (MDA) and glutathione (GSH). Additionally, the activities of myeloperoxidase (MPO) catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as histological changes, were assessed. The findings demonstrated that TT/D significantly increased the FSH and LH levels, reduced sperm quality and testosterone levels, elevated MDA levels, and enhanced MPO activity. Additionally, TT/D reduced the GSH levels and the activities of GPx, CAT, and SOD. Probiotic treatment, particularly with <i>L. rhamnosus</i>, significantly increased testicular GSH levels and the activities of antioxidant enzymes GPx and CAT while reducing MDA levels and MPO activity. Histopathological analysis revealed severe testicular damage, including reductions in spermatogenic, sertoli, and Leydig cells following TT/D, mitigated by <i>L. rhamnosus</i>. These results suggest that probiotics, especially <i>L. rhamnosus</i>, may protect against I/R injury in a rat model of testicular T/D, likely due to their antioxidant properties.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8508956","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}