地塞米松诱导的慢性应激通过上皮细胞凋亡降低生化液体成分和精囊分泌蛋白4的表达

IF 2 4区 医学 Q3 ANDROLOGY
Andrologia Pub Date : 2025-09-09 DOI:10.1155/and/7168333
Sararat Innoi, Tidarat Chawalchitiporn, Tarinee Sawatpanich, Nareelak Tangsrisakda, Chadaporn Chaimontri, Chayakorn Taoto, Therachon Kamollerd, Yutthaphong Patjorn, Apichaya Wongsomwong, Auekarn Chaiyamart, Wipawee Thukhammee, Chanasorn Poodendaen, Suthat Duangchit, Sitthichai Iamsaard
{"title":"地塞米松诱导的慢性应激通过上皮细胞凋亡降低生化液体成分和精囊分泌蛋白4的表达","authors":"Sararat Innoi,&nbsp;Tidarat Chawalchitiporn,&nbsp;Tarinee Sawatpanich,&nbsp;Nareelak Tangsrisakda,&nbsp;Chadaporn Chaimontri,&nbsp;Chayakorn Taoto,&nbsp;Therachon Kamollerd,&nbsp;Yutthaphong Patjorn,&nbsp;Apichaya Wongsomwong,&nbsp;Auekarn Chaiyamart,&nbsp;Wipawee Thukhammee,&nbsp;Chanasorn Poodendaen,&nbsp;Suthat Duangchit,&nbsp;Sitthichai Iamsaard","doi":"10.1155/and/7168333","DOIUrl":null,"url":null,"abstract":"<p><b>Background:</b> Dexamethasone (DEX) has recently been used to treat inflammation and to induce the chronic stress (CS) in animal models. Although its adverse effects on testicular damage and seminal plasma reduction were previously reported, the changes in the seminal vesicle are still unexplored. This study aimed to investigate the effects of DEX on biochemical alterations in seminal vesicle fluid (SVF) and seminal vesicle tissue (SVT).</p><p><b>Methods:</b> Male rats were divided into control and DEX groups (<i>n</i> = 20). CS animals were induced with DEX at 1.5 mg/KgBW for 21 consecutive days. CS behaviors were confirmed by the tests of tail suspension, forced swimming, and sucrose preference. Hormones and biochemical components in serum and SVF were evaluated. SVF volume and histomorphometry of SVT were observed. Expressions of apoptotic markers and seminal vesicle secreting protein 4 (SVS4) were determined.</p><p><b>Results:</b> The results showed the reductions of seminal vesicle size and SVF volume in DEX rats. DEX increased the malondialdehyde (MDA) level in SVF, and DNA fragmentation revealed by the TUNEL assay. Serum testosterone and levels of magnesium and fructosamine in SVF of the DEX group were significantly decreased. DEX altered the thickness of the seminal vesicle wall. The expression of SVS4 was decreased in SVT induced with DEX. However, no difference in apoptotic expressions (Hsp70, procaspase-3, and procaspase-9) was observed between groups.</p><p><b>Conclusion:</b> The DEX-induced CS caused the seminal vesicle changes, which were associated with DNA fragmentation. Decreases of fructosamine and SVS4 may be a cause of low-sperm quality such as motility and capacitation in CS men.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7168333","citationCount":"0","resultStr":"{\"title\":\"Dexamethasone-Induced Chronic Stress Decreases Biochemical Fluid Components and Seminal Vesicle Secreting Protein 4 Expression Via Epithelial Cell Apoptosis\",\"authors\":\"Sararat Innoi,&nbsp;Tidarat Chawalchitiporn,&nbsp;Tarinee Sawatpanich,&nbsp;Nareelak Tangsrisakda,&nbsp;Chadaporn Chaimontri,&nbsp;Chayakorn Taoto,&nbsp;Therachon Kamollerd,&nbsp;Yutthaphong Patjorn,&nbsp;Apichaya Wongsomwong,&nbsp;Auekarn Chaiyamart,&nbsp;Wipawee Thukhammee,&nbsp;Chanasorn Poodendaen,&nbsp;Suthat Duangchit,&nbsp;Sitthichai Iamsaard\",\"doi\":\"10.1155/and/7168333\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><b>Background:</b> Dexamethasone (DEX) has recently been used to treat inflammation and to induce the chronic stress (CS) in animal models. Although its adverse effects on testicular damage and seminal plasma reduction were previously reported, the changes in the seminal vesicle are still unexplored. This study aimed to investigate the effects of DEX on biochemical alterations in seminal vesicle fluid (SVF) and seminal vesicle tissue (SVT).</p><p><b>Methods:</b> Male rats were divided into control and DEX groups (<i>n</i> = 20). CS animals were induced with DEX at 1.5 mg/KgBW for 21 consecutive days. CS behaviors were confirmed by the tests of tail suspension, forced swimming, and sucrose preference. Hormones and biochemical components in serum and SVF were evaluated. SVF volume and histomorphometry of SVT were observed. Expressions of apoptotic markers and seminal vesicle secreting protein 4 (SVS4) were determined.</p><p><b>Results:</b> The results showed the reductions of seminal vesicle size and SVF volume in DEX rats. DEX increased the malondialdehyde (MDA) level in SVF, and DNA fragmentation revealed by the TUNEL assay. Serum testosterone and levels of magnesium and fructosamine in SVF of the DEX group were significantly decreased. DEX altered the thickness of the seminal vesicle wall. The expression of SVS4 was decreased in SVT induced with DEX. However, no difference in apoptotic expressions (Hsp70, procaspase-3, and procaspase-9) was observed between groups.</p><p><b>Conclusion:</b> The DEX-induced CS caused the seminal vesicle changes, which were associated with DNA fragmentation. Decreases of fructosamine and SVS4 may be a cause of low-sperm quality such as motility and capacitation in CS men.</p>\",\"PeriodicalId\":7817,\"journal\":{\"name\":\"Andrologia\",\"volume\":\"2025 1\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7168333\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Andrologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/and/7168333\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ANDROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Andrologia","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/and/7168333","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ANDROLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:地塞米松(Dexamethasone, DEX)最近在动物模型中被用于治疗炎症和诱导慢性应激(chronic stress, CS)。虽然其对睾丸损伤和精浆减少的不良影响已被报道,但对精囊的影响仍未见报道。本研究旨在探讨DEX对精囊液(SVF)和精囊组织(SVT)生化变化的影响。方法:雄性大鼠分为对照组和DEX组(n = 20)。以1.5 mg/KgBW的DEX诱导CS动物,连续21 d。CS行为通过悬尾、强迫游泳和蔗糖偏好试验得到证实。测定血清及SVF中激素及生化成分。观察SVF体积及SVT组织形态学变化。检测细胞凋亡标志物及精囊分泌蛋白4 (SVS4)的表达。结果:结果显示DEX大鼠精囊大小和SVF体积减小。DEX增加了SVF中的丙二醛(MDA)水平,并通过TUNEL检测显示DNA片段化。DEX组血清睾酮水平和SVF中镁、果糖胺水平均显著降低。DEX改变精囊壁厚度。DEX诱导的SVS4表达降低。然而,各组细胞凋亡(Hsp70、procaspase-3、procaspase-9)表达无差异。结论:dex诱导的CS引起精囊改变,且与DNA断裂有关。果糖胺和SVS4的减少可能是CS男性精子质量低的原因,如活力和能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dexamethasone-Induced Chronic Stress Decreases Biochemical Fluid Components and Seminal Vesicle Secreting Protein 4 Expression Via Epithelial Cell Apoptosis

Dexamethasone-Induced Chronic Stress Decreases Biochemical Fluid Components and Seminal Vesicle Secreting Protein 4 Expression Via Epithelial Cell Apoptosis

Background: Dexamethasone (DEX) has recently been used to treat inflammation and to induce the chronic stress (CS) in animal models. Although its adverse effects on testicular damage and seminal plasma reduction were previously reported, the changes in the seminal vesicle are still unexplored. This study aimed to investigate the effects of DEX on biochemical alterations in seminal vesicle fluid (SVF) and seminal vesicle tissue (SVT).

Methods: Male rats were divided into control and DEX groups (n = 20). CS animals were induced with DEX at 1.5 mg/KgBW for 21 consecutive days. CS behaviors were confirmed by the tests of tail suspension, forced swimming, and sucrose preference. Hormones and biochemical components in serum and SVF were evaluated. SVF volume and histomorphometry of SVT were observed. Expressions of apoptotic markers and seminal vesicle secreting protein 4 (SVS4) were determined.

Results: The results showed the reductions of seminal vesicle size and SVF volume in DEX rats. DEX increased the malondialdehyde (MDA) level in SVF, and DNA fragmentation revealed by the TUNEL assay. Serum testosterone and levels of magnesium and fructosamine in SVF of the DEX group were significantly decreased. DEX altered the thickness of the seminal vesicle wall. The expression of SVS4 was decreased in SVT induced with DEX. However, no difference in apoptotic expressions (Hsp70, procaspase-3, and procaspase-9) was observed between groups.

Conclusion: The DEX-induced CS caused the seminal vesicle changes, which were associated with DNA fragmentation. Decreases of fructosamine and SVS4 may be a cause of low-sperm quality such as motility and capacitation in CS men.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Andrologia
Andrologia 医学-男科学
CiteScore
5.60
自引率
8.30%
发文量
292
审稿时长
6 months
期刊介绍: Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信