A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells

IF 2.1 4区 医学 Q3 ANDROLOGY
Andrologia Pub Date : 2024-02-12 DOI:10.1155/2024/8061627
Kiana Sojoudi, Hossein Azizi, Thomas Skutella
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引用次数: 0

Abstract

Krüppel-like factor 4 (Klf4) is a transcription factor involved in proliferation, self-renewal, differentiation, apoptosis, and somatic cell reprogramming; thus, it has a critical role in accurate spermatogenesis. In this study, we examine the different protein localizations of Klf4 in the mouse testis and in two populations of spermatogonial stem cells (SSCs), including differentiated and undifferentiated cell types. We show that in contrast to its function during spermatogenesis in the adult testis, in vitro Klf4 is a factor in maintaining stemness in different SSC populations. Furthermore, we suggest that its functions could be age-dependent. Bioinformatics analysis in the Cytoscape platform was conducted to highlight pathways and networks of Klf4 interactions. Our results support the different functions of Klf4 dependent on the age and differentiation state of SSCs.

精原干细胞分化和维持多能性的两个不同过程之间 Klf4 的双重功能和表达模式研究
Krüppel样因子4(Klf4)是一种转录因子,参与增殖、自我更新、分化、凋亡和体细胞重编程;因此,它在准确的精子发生中起着至关重要的作用。在这项研究中,我们研究了Klf4在小鼠睾丸和两种精原干细胞(包括已分化和未分化细胞类型)中的不同蛋白定位。我们发现,与其在成体睾丸精子发生过程中的功能不同,体外Klf4是维持不同SSC群体干性的一个因素。此外,我们还发现其功能可能与年龄有关。我们在Cytoscape平台上进行了生物信息学分析,以突出Klf4相互作用的途径和网络。我们的结果支持Klf4的不同功能取决于SSCs的年龄和分化状态。
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来源期刊
Andrologia
Andrologia 医学-男科学
CiteScore
5.60
自引率
8.30%
发文量
292
审稿时长
6 months
期刊介绍: Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
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