Andrologia最新文献

{"title":"Clinical Pregnancy and Live Birth Outcomes after Intracytoplasmic Injection of Fresh versus Frozen Testicular Sperm","authors":"Yuqin Zhu,&nbsp;Nengyong Ouyang,&nbsp;Xiaohui Ji,&nbsp;Qingxue Zhang,&nbsp;Haijing Zhao","doi":"10.1155/2024/3802703","DOIUrl":"10.1155/2024/3802703","url":null,"abstract":"<p>This study aimed to investigate the potential benefits of frozen testicular sperm and to retrospectively analyze the clinical pregnancy and live birth outcomes following intracytoplasmic injection of fresh or frozen testicular sperm. A total of 468 infertile couples undergoing intracytoplasmic sperm injection cycles utilizing either fresh or frozen testicular sperm extracted between January 2017 and December 2021 were included in this analysis. Participants were categorized into two groups: those utilizing fresh testicular sperm (<i>n</i> = 324) and those utilizing frozen testicular sperm (<i>n</i> = 144). Outcome measures encompassed fertilization rate, embryo development, clinical outcomes, and birth status of infants. Statistical analysis revealed no significant differences in 2PN fertilization rate, Day 3 (D3) available embryo rate, high-quality embryo rate, blastocyst formation rate, clinical pregnancy rate, live birth rate, and preterm birth rate between the two groups. Although the preterm birth rate in the frozen testicular sperm group was lower compared to the fresh testicular sperm group, the difference was not statistically significant (5.6% vs. 15.1%). Notably, there were no discernible distinctions in clinical pregnancy and birth outcomes, as well as infant birth parameters, between the fresh and frozen testicular sperm groups. These findings suggest that frozen testicular sperm holds practical value and warrants consideration for clinical application.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140680304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MTHFRC 677T Gene Polymorphism and Homocysteine in North China Patients with Varicocele","authors":"Yiping Xiu,&nbsp;Zhiqiang Cao,&nbsp;Dongyang Yu,&nbsp;Caleb Yang,&nbsp;Jiang Wang,&nbsp;Jiancheng Pan,&nbsp;Zhongcheng Xin,&nbsp;Yuhong Feng","doi":"10.1155/2024/3576162","DOIUrl":"10.1155/2024/3576162","url":null,"abstract":"<p>To investigate the correlation between methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism, homocysteine, and male infertility with varicocele in North China. One Hundred infertile males with varicocele (VC; grade II-III, VC group) and 100 healthy males with normal semen parameters and no varicocele (NC group) were recruited for PCR microarray, blood and semen testing. Compared with the CC genotype in the NC group, the TT genotype in the NC group and the CC genotype in the VC group showed no significant changes in sperm motility (<i>P</i> = 0.191; <i>P</i> = 0.130), sperm density (<i>P</i> = 0.591, <i>P</i> = 0.643), plasma homocysteine level (<i>P</i> = 0.511; <i>P</i> = 0.677), and seminal plasma MDA (<i>P</i> = 0.752; <i>P</i> = 0.451). In contrast, VC patients with the TT genotype had higher plasma homocysteine level and seminal plasma MDA levels (<i>P</i>  &lt; 0.001), lower partial pressure of oxygen in seminal pulse (PO₂; <i>P</i>  &lt; 0.001) and poorer sperm quality (<i>P</i>  &lt; 0.001), as compared with the CC genotype. This suggests that MTHFR C677C&gt;T may not be a risk factor for male Varicocele in North China. However, this may affect the oxidative stress associated with homocysteine expression, which in turn affects semen parameters in VC patients. Larger studies are needed to validate our findings.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140721637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-Rapid Freezing and Rapid Freezing Methods in Clinical ICSI Program: Effects on Sperm Biological Characteristics, DNA Methylation Stability, DNA Methyltransferase Activity, and Embryo Morphokinetics","authors":"Marzieh Zohrabi,&nbsp;Mohammad Ali Khalili,&nbsp;Esmat Mangoli,&nbsp;Fateme Zare,&nbsp;Bryan Woodward,&nbsp;Behrouz Aflatoonian","doi":"10.1155/2024/2789285","DOIUrl":"10.1155/2024/2789285","url":null,"abstract":"<p>The purpose of this study was to evaluate the differences in sperm function and embryo morphokinetics following sperm cryopreservation by ultra-rapid freezing or rapid freezing methods compared to fresh spermatozoa. Thirty samples of normozoospermia were divided equally into fresh, ultra-rapid freezing, and rapid freezing groups. In the rapid freezing, sperm suspension was placed horizontally on nitrogen vapor. In the ultra-rapid freezing, sperm suspension with a straw-in-straw system was directly immersed in liquid nitrogen. Sperm function was assessed in terms of motility, morphology, viability, mitochondrial membrane potential, sperm DNA fragmentation, and acrosome reaction status. Also, the effects of two cryopreservation methods were assessed on global DNA methylation and DNA methyltransferase activity. Moreover, 730 embryos in three groups were cultured using time-lapse imaging until day 6 for embryo morphokinetics. Progressive motility (38.80 ± 4.21 vs. 34.86 ± 4.19; <i>p</i>  &lt; 0.001) and viability (64.30 ± 6.24 vs. 58.10 ± 8.69; <i>p</i> &lt; 0.01) in ultra-rapid freezing were significantly higher than rapid group. DNA fragmentation and acrosome reaction were significantly increased in both cryopreserved groups (<i>p</i>  &lt; 0.001). However, DNA fragmentation (16.30 ± 1.14 vs. 14.33 ± 2.94; <i>p</i> &lt; 0.01) was significantly higher in the rapid than the ultra-rapid freezing group. No significant differences were noted in global DNA methylation (<i>p</i> &gt; 0.05) and DNA methyltransferases activity (<i>p</i> &gt; 0.05) in fresh compared to cryopreservation groups. The kinetic times, including tPB2, tPNa, tPNf, t2, t3, t4, t5, t6, t7, t8, and tM, showed a significant delay in cell divisions in both cryopreservation groups. Furthermore, tPNa, tPNf, and t8 occurred with a significantly higher delay in embryos fertilized by sperm from the rapid freezing compared to the ultra-rapid freezing group. In addition, blastocysts formation was similar in both cryopreservation groups. Ultra-rapid freezing preserved the sperm biological integrity and lead to better embryo morphokinetics compared to the rapid freezing method. However, both methods of sperm cryopreservation were epigenetically safe.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mood Disorders and Sexual Function in Infertile Men: Exploring the Relationship with Semen Analysis Results","authors":"Maryam Nakhaee Moghadam,&nbsp;Marjan Ghaemi,&nbsp;Sina Eskandari Delfan,&nbsp;Maryam Afshari,&nbsp;Mahin Kaveh,&nbsp;Nasim Eshraghi,&nbsp;Zahra Shahraki","doi":"10.1155/2024/1514237","DOIUrl":"10.1155/2024/1514237","url":null,"abstract":"<p><i>Objective</i>. The presence of infertility in couples not only results in heightened stress but also elevates the risk of developing psychological disorders, further exacerbating their situation. This study is designed to evaluate the relationship between semen analysis and depression, anxiety, and stress, as well as sexual function in infertile men. <i>Method</i>. This descriptive cross-sectional study was carried out at Amir Al-Momenin Hospital of Zabol between March 2019 and April 2020. The couples who were candidates for intracytoplasmic sperm injection according to male factors were recruited for the study. One hundered two men were asked to complete the depression, anxiety, and stress questionnaire (DASS-42) and the International Index of Erectile Function questionnaire (IIEF-15). The findings of the DASS and IIEF questionnaires were compared with the semen analysis test and reported. <i>Results</i>. The frequency of depression, anxiety, and moderate to very severe stress were found in 21 (20.6%), 40 (39.2%), and 23 (22.5%) cases, respectively. Surprisingly, 60 (59%) participants had erectile dysfunction. A significant correlation was found between mood disorders (depression, anxiety, and stress) and some components of the erectile function questionnaire, stress with erectile function (<i>p</i> = 0.045), depression with overall satisfaction (<i>p</i> = 0.011), and anxiety with intercourse satisfaction (<i>p</i> = 0.048) and overall satisfaction (<i>p</i> = 0.037). Also, there was a significant correlation between semen analysis parameters such as sperm motility with depression (<i>p</i> = 0.035) and anxiety (<i>p</i> = 0.037), normal sperm morphology with stress (<i>p</i> = 0.034), progressive sperm motility with orgasmic function (<i>p</i> = 0.006), and intercourse satisfaction (<i>p</i> = 0.014). <i>Conclusion</i>. The higher the mental health, the higher the erectile function. Improvement of mood disorders leads to improvement of semen parameters and increases the chances of pregnancy. Psychological support may be helpful so, in addition to medical treatment, these people should also receive supportive psychological treatment.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of RIP1/RIP3 Necroptosis Pathway Promote Erectile Function in Cold-Stressed Rat Model","authors":"Pei Yang,&nbsp;Lipan Niu,&nbsp;Bingbing Zhu,&nbsp;Siyiti Amuti,&nbsp;Adilijiang Yiming,&nbsp;Fengxia Liu","doi":"10.1155/2024/9483858","DOIUrl":"10.1155/2024/9483858","url":null,"abstract":"<p>Cold stimulation is the most common stressor in cold regions. Continuous cold stimulation can cause a series of pathophysiological changes in the body, such as aggregated neutrophils, macrophage activation, and increased inflammatory factors, which is also a risk factor for erectile function impairment. In addition, necroptosis is an important form of programmed cell death. However, the mechanisms of necroptosis in erectile function impairment due to cold stimulation have been very poorly studied. Therefore, we explored the mechanism of tumor necrosis factor-<i>α</i> (TNF-<i>α</i>)-mediated receptor interacting protein kinase 1 (RIP1)/receptor interacting protein kinase 3 (RIP3) necroptosis pathway on erectile function among cold-stressed rats. First, we established a cold-stressed rat model using cold stimulation and selected those rats that had developed erectile function impairment. Then, we used Necrostatin-1 (RIP1 specific inhibitor, Nec-1), Etanercept (TNF-<i>α</i> inhibitor, Ent), and Sildenafil (Sil) to intervene for 14 days and subsequently assessed their erectile function by apomorphine test and sexual behavioural test. Lastly, we performed various molecular studies and histopathological analyses of penile tissues collected from these rats after the experiments. We found that erectile function was impaired in cold-stressed rats, with increased penile tissue fibrosis and elevated levels of TNF-<i>α</i> and necroptosis. Contrastingly, intervention with Nec-1 and Ent restored erectile function, reduced penile tissue fibrosis, and decreased TNF-<i>α</i> and necroptosis levels, consistent with the results of intervention with Sil. Based on these results, we confirmed that the TNF-<i>α</i>-mediated RIP1/RIP3 necroptosis pathway was significantly altered in cold-stressed rats. In conclusion, inhibition of the TNF-<i>α</i>-mediated RIP1/RIP3 necroptosis pathway improved erectile function, suggesting that the specific downstream mechanisms need to be further explored.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140366154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Erectile Dysfunction among Patients with Coronary Artery Disease","authors":"Islam Mohamed Abd Elsamie,&nbsp;Hassan Abou Khodair,&nbsp;Mohamed Sayed Bashandy,&nbsp;Hany Aboelwafa","doi":"10.1155/2024/6151669","DOIUrl":"https://doi.org/10.1155/2024/6151669","url":null,"abstract":"<p>Both coronary artery disease (CAD) and erectile dysfunction (ED) are widespread illnesses that frequently coexist. Numerous triggering risk factors and pathophysiological mechanisms contribute to both diseases. Penile color Doppler ultrasound can serve as a crucial tool to evaluate penile arterial blood flow. Therefore, this study aims to assess the ED prevalence in CAD patients, to determine the socioeconomic and clinical profiles of the population, and to define risk factors of their development. The cross-sectional study included 100 male patients who were admitted to the coronary angiography (CAG) unit. Patients were asked if they had ED, and if present, it was graded using the International Index of Erectile Function 5. Right and left cavernosal arteries were examined using color Doppler and erection hardness to assess the peak systolic velocity (PSV) and end-diastolic velocity. Among the 52 cases that suffered from ED, most of the cases had mild grades (38.5%). All cases without ED (100%) had penile artery Doppler PSV findings ≥30 (cm/s) with an average Doppler PSV of 48.02 ± 7.49 cm/s for the right cavernosal arteries and 47.4 ± 7.17 cm/s for the left cavernosal arteries. Yet, 71.2% of cases with ED had penile artery Doppler PSV findings &lt;30 (cm/s) with an average Doppler PSV of 29.1 ± 7.57 cm/s for the right cavernosal arteries and 28.69 ± 7.11 cm/s for the left cavernosal arteries. Furthermore, by CAG, 50% of cases without ED had blockage not necessitating a coronary intervention (no significant disease) compared to 25% of cases with ED. There was positive correlation between advanced grade of ED and age (<i>r</i> = 0.327, <i>P</i>  &lt; 0.001), hypertension (<i>r</i> = 0.213, <i>P</i> = 0.033), DM (<i>r</i> = 0.253, <i>P</i> = 0.011), smoking (<i>r</i> = 0.202, <i>P</i> = 0.044), and severity of CAD (<i>r</i> = 0.332, <i>P</i>  &lt; 0.001). Patients with multivessel CAD have a significantly higher incidence and more severity of ED. Moreover, pathological penile vessels PSV (&lt;25 or &lt;30 cm/s) via Doppler ultrasound is mostly valuable as a noninvasive tool to define ED attributable to vascular impairment that may be antecedent to atherosclerosis in the coronary vessels.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Profortil® on Leptin-Induced Adverse Effects on Sperm Parameters in Sprague-Dawley Rats","authors":"Ifrah Alam Malik,&nbsp;Damayanthi Durairajanayagam,&nbsp;Ralf Henkel,&nbsp;Harbindar Jeet Singh","doi":"10.1155/2024/6122804","DOIUrl":"10.1155/2024/6122804","url":null,"abstract":"<p>Raised leptin levels induce oxidative stress, which negatively impacts male reproduction. Profortil® is an antioxidant formulation clinically used to improve sperm quality. Whether Profortil® supplementation can prevent the detrimental effects of leptin on spermatozoa is not known. Thus, the aim of this study was to examine the effects of Profortil® on leptin-induced adverse effects on rat spermatozoa. Adult Sprague-Dawley rats were given either normal saline (control), leptin (60 <i>µ</i>g/kg/day), Profortil® (50 mg/kg/day), or Profortil® + leptin once daily for 2 weeks. Sperm count and abnormal morphology were recorded. Testicular levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), superoxide dismutase (SOD), and catalase (CAT) activities were measured. TUNEL and Comet assays were performed. The sperm count in the Profortil® + leptin group was comparable to that in controls but was significantly higher than that in the leptin group, which was significantly lower than that in controls. Sperm morphology was not different between leptin and Profortil® + leptin groups. 8-OHdG level was significantly lower in the Profortil® + leptin group than that in the leptin group but was comparable to that in controls. CAT activity was not different between leptin and Profortil® + leptin groups, although activity in the former was significantly lower than that in controls. SOD activity was not different between groups. The apoptotic index was significantly higher in the leptin and Profortil® + leptin groups than in the control and Profortil® groups. Sperm DNA fragmentation was not significantly different between leptin and Profortil® + leptin groups. In conclusion, Profortil®, when given at 50 mg/kg/day for 3 weeks, was only able to reduce some, but not all the adverse effects of leptin on sperm parameters.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140218873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Astaxanthin on Post-Thaw Sperm Quality in Normozoospermic Men","authors":"Afshin Mohammadi-Bardbori,&nbsp;Amir Shadboorestan,&nbsp;Ensieh Salehi,&nbsp;Mohammad Amin Rahimi,&nbsp;Elias Kargar-Abarghouei,&nbsp;Fardin Amidi,&nbsp;Jebreil Shamseddin,&nbsp;Mahmoud Omidi","doi":"10.1155/2024/2332443","DOIUrl":"10.1155/2024/2332443","url":null,"abstract":"<p>Fighting against free radical accumulation during the cryopreservation process with the help of exogenous antioxidants has become an effective strategy to augment post-thaw sperm quality. The present study explored the effects of various concentrations of astaxanthin, as a potent antioxidant, on human sperm parameters during the freeze–thaw process. Twenty-five normozoospermic specimens were included in this prospective study. Each sample was divided into five equal parts: fresh group, and frozen–thawed groups including 0, 1, 10, and 50 <i>μ</i>M of astaxanthin. The prepared spermatozoa were cryopreserved by rapid freezing technique. Precryopreservation and post-thaw sperm motility parameters, sperm morphology, vitality, DNA fragmentation, reactive oxygen species (ROS) levels, and mitochondrial membrane potential (MMP) were investigated. All sperm parameters after thawing significantly decreased compared to before freezing. Treatment of spermatozoa with 50 <i>μ</i>M of astaxanthin significantly increased their total and progressive motility, viability, DNA integrity, and MMP and decreased their intracellular ROS levels compared with the control group. In total, 10 <i>μ</i>M of astaxanthin significantly improved total and progressive motility, DNA integrity, MMP, and decreased ROS levels, whereas, in the 1 <i>µ</i>M group, there were significant differences only in ROS levels. As a result, we found that astaxanthin can improve sperm quality after freezing/thawing and decrease the detrimental effects of this process on sperm parameters.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140225438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Endogenous Seminal L-Carnitine Levels with Post-Thaw Semen Parameters in Humans","authors":"Mario Iliceto,&nbsp;Jorunn M. Andersen,&nbsp;Mette Haug Stensen,&nbsp;Trine B. Haugen,&nbsp;Oliwia Witczak","doi":"10.1155/2024/4327010","DOIUrl":"10.1155/2024/4327010","url":null,"abstract":"<p>Cryopreservation of semen is a useful tool for male fertility preservation. Some evidence for a beneficial effect of L-carnitine supplementation of freezing media on cryopreserved semen samples has been reported. Here, we examined the association of endogenous levels of seminal L-carnitine with post-thaw semen parameters. We also investigated the effect of freezing medium supplemented with L-carnitine on sperm characteristics, related to endogenous seminal L-carnitine levels. Semen analyses were performed on 125 fresh samples, and after standard cryopreservation and with L-carnitine as a supplement. Participants were categorized into two groups based on the median levels of endogenous seminal L-carnitine: low L-carnitine (≤38.8 <i>µ</i>g/ml) and high L-carnitine (&gt;38.8 <i>µ</i>g/ml). After standard cryopreservation, semen samples with high L-carnitine levels showed higher rapid progressive, progressive and total sperm motility and a reduced seminal static oxidation–reduction potential (ORP) level than samples with low L-carnitine levels. Only in post-thaw samples with low L-carnitine levels, there was an increase in the amount of sperm neck midpiece defects, compared to the fresh samples. Cryopreservation with L-carnitine had the most beneficial effect on rapid progressive sperm motility in samples with high endogenous L-carnitine levels. In conclusion, L-carnitine has a beneficial impact on sperm characteristics in post-thaw samples both as an endogenous component in seminal plasma and as a supplement in the freezing medium, by improving sperm motility and reducing seminal oxidative stress.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140085397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsurgical Testicular Sperm Extraction: Predictive Factors and Outcomes for Men with Nonobstructive Azoospermia","authors":"B. Mahdy,&nbsp;G. La Croce,&nbsp;M. Roscigno,&nbsp;M. Manica,&nbsp;L. Da Pozzo,&nbsp;A. Sacca","doi":"10.1155/2024/6380023","DOIUrl":"10.1155/2024/6380023","url":null,"abstract":"<p><i>Background</i>. Nonobstructive azoospermia (NOA) is a frequent cause of male infertility, and microsurgical testicular sperm extraction (micro-TESE) is an effective technique for sperm retrieval in NOA cases. However, the predictive factors for successful sperm retrieval and the outcomes of micro-TESE in NOA patients are not fully understood. <i>Methods</i>. In this retrospective study, we evaluated the predictive value of preoperative data and outcomes of micro-TESE in 172 men with NOA who underwent the procedure between April 2012 and February 2021. Patients’ age, medical history, testicular size, hormonal profile, testicular histology, and sperm retrieval were recorded. <i>Results</i>. 172 cases of micro-TESE were performed (99 unilateral and 73 bilateral) and testicular sperm retrieval rate (SRR) was 72.7%. There was no statistical difference in age, testicular size, and hormonal profiles between positive SRR and negative SRR groups. Clinical varicocele and previous orchitis did not significantly affect the result of micro-TESE. Y chromosome microdeletions were detected in seven patients. On histological examination, hypospermatogenesis was found in 39% of patients who underwent testicular biopsy. SRR was higher in patients with hypospermatogenesis than in other groups. <i>Conclusions</i>. Testicular microdissection is an effective and safe procedure for nonobstructive azoospermia patients, regardless of the different etiologies and preoperative data. Age, testicular size, and hormonal levels did not predict the success of micro-TESE. The presence of hypospermatogenesis was associated with a higher SRR. Clinical varicocele and previous orchitis were not significantly associated with the outcome of micro-TESE.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2024 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140438833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}