AndrologiaPub Date : 2026-04-01DOI: 10.1155/and/6243303
Kiafon Betrand Nsah, Nguedem Sylvie Fonkwo, Njoya Moses Tita Mogho, Tangu Patience Neng, Mumbi Laurantine Ngenteh, Ndifor Rose Nchang, Bih Belta Lilian Fubi, Kada Sanda Antoine
{"title":"Protective Effects of the Aqueous Extract of Tribulus cistoides (Zygophyllaceae) Against Nicotine-Induced Fertility Impairment in Male Wistar Rats","authors":"Kiafon Betrand Nsah, Nguedem Sylvie Fonkwo, Njoya Moses Tita Mogho, Tangu Patience Neng, Mumbi Laurantine Ngenteh, Ndifor Rose Nchang, Bih Belta Lilian Fubi, Kada Sanda Antoine","doi":"10.1155/and/6243303","DOIUrl":"https://doi.org/10.1155/and/6243303","url":null,"abstract":"<p>Infertility affects a substantial proportion of couples worldwide and is defined as the failure to achieve pregnancy after 1 year of regular, unprotected sexual intercourse. Growing interest has been directed toward ethnobotanical approaches for the management of male infertility. This study evaluated the therapeutic potential of the aqueous extract of <i>Tribulus cistoides</i> against nicotine-induced infertility in male Wistar rats. Thirty adult male rats were randomly allocated into six groups (<i>n</i> = 5). Group 1 received distilled water (5 mL/kg), Group 2 received nicotine alone (1 mg/kg), and Group 3 received nicotine plus testosterone enanthate (5 mg/kg), while Groups 4–6 were administered nicotine in combination with <i>T. cistoides</i> aqueous extract at doses of 50, 100, and 150 mg/kg, respectively, for a period of 60 days. Evaluated parameters included body and reproductive organ weights, testicular oxidative stress markers, seminal vesicle fructose, total testicular protein, sperm density and motility, serum testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testicular histoarchitecture. Nicotine administration resulted in a significant reduction in sperm density (15.00 ± 1.05 × 10<sup>6</sup>/g vs. 43.05 ± 3.06 × 10<sup>6</sup>/g), sperm motility (35.73% ± 0.72% vs. 56.44% ± 2.17%), and serum testosterone levels (0.124 ± 0.002 ng/mL vs. 0.521 ± 0.048 ng/mL) compared with the normal control group. Conversely, treatment with <i>T. cistoides</i> extract at 150 mg/kg significantly improved sperm density (50.15 ± 1.46 × 10<sup>6</sup>/g), motility (71.16% ± 4.15%), and testosterone levels (0.411 ± 0.016 ng/mL). In contrast, testosterone enanthate did not produce significant improvements in the fertility parameters assessed when compared with the nicotine-treated group. The extract also enhanced testicular antioxidant status, reproductive hormone profiles, seminal fructose levels, testicular protein content, and reproductive organ weights, likely due to its androgenic and antioxidant properties. Histological examination further revealed increased sperm density in the extract-treated groups like in the normal control group. In conclusion, the aqueous extract of <i>T. cistoides</i> exhibited notable protective and restorative effects against nicotine-induced male infertility, highlighting its potential as a natural therapeutic agent for improving male reproductive function.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6243303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147683089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Melatonin Addition During Cryopreservation of Chinchilla Rabbit Spermatozoa in Mexico","authors":"Daniel González-Ruiz, Metzli Mayte García-Bernal, Elisa Gutiérrez Hernández, Alicia Alcántar-Rodríguez, Alfredo Medrano","doi":"10.1155/and/1591567","DOIUrl":"https://doi.org/10.1155/and/1591567","url":null,"abstract":"<p>In recent years, efforts have been made to make rabbit production more efficient, and reproductive strategies, such as artificial insemination, have been developed to make it more productive. However, unlike in other species, there are currently no protocols to ensure that sperm quality is maintained during thawing. As the main problem faced by spermatozoa during cryopreservation is free radical formation, the addition of antioxidants, such as melatonin (MLT), to extenders for sperm cryopreservation has been tested. This study aimed to evaluate the effects of different MLT concentrations on the cryopreservation of Chinchilla rabbit sperm in Mexico. Ejaculates were collected from six males with proven fertility, and a pool was formed from those that had at least 70% progressive motility (PM) (18 pools in total). The pooled semen samples were microscopically assessed for wave motion (WM), PM, viability (VB), morphology, membrane functionality (MF), and acrosomal integrity. A two-step freezing protocol was applied using INRA-82 as the extender supplemented with Orvus Paste (0.5%), dimethyl sulfoxide (DMSO) (4%), and dimethylformamide (DMF) (4%) as cryoprotectants (final concentration). Aliquots were taken to form six MLT concentrations (0.0, 0.1, 0.75, 1.5, 2.25 and 3.0 mM) at room temperature (23°C). The sperm samples were packaged in 0.25 mL straws, cooled to 5°C, and then exposed to nitrogen vapours before being immersed in liquid nitrogen for storage. For thawing, the straws were immersed in a water bath for 30 s at 37°C. Thawed sperm samples were microscopically assessed for VB using the NucleoCounter (NC) system, following the manufacturer’s instructions. Analysis of variance (ANOVA) was conducted to compare the effects of different MLT concentrations on sperm parameters. No significant differences (<i>p</i> > 0.05) were observed among the concentrations for any of the evaluated variables. In conclusion, MLT addition during Chinchilla rabbit sperm cryopreservation had no effect on sperm motility, VB, acrosomal integrity, MF, membrane integrity, or morphology.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2026 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1591567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147684277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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