MTHFRC 677T Gene Polymorphism and Homocysteine in North China Patients with Varicocele

IF 2.1 4区 医学 Q3 ANDROLOGY
Andrologia Pub Date : 2024-04-09 DOI:10.1155/2024/3576162
Yiping Xiu, Zhiqiang Cao, Dongyang Yu, Caleb Yang, Jiang Wang, Jiancheng Pan, Zhongcheng Xin, Yuhong Feng
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Abstract

To investigate the correlation between methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism, homocysteine, and male infertility with varicocele in North China. One Hundred infertile males with varicocele (VC; grade II-III, VC group) and 100 healthy males with normal semen parameters and no varicocele (NC group) were recruited for PCR microarray, blood and semen testing. Compared with the CC genotype in the NC group, the TT genotype in the NC group and the CC genotype in the VC group showed no significant changes in sperm motility (P = 0.191; P = 0.130), sperm density (P = 0.591, P = 0.643), plasma homocysteine level (P = 0.511; P = 0.677), and seminal plasma MDA (P = 0.752; P = 0.451). In contrast, VC patients with the TT genotype had higher plasma homocysteine level and seminal plasma MDA levels (P  < 0.001), lower partial pressure of oxygen in seminal pulse (PO2; P  < 0.001) and poorer sperm quality (P  < 0.001), as compared with the CC genotype. This suggests that MTHFR C677C>T may not be a risk factor for male Varicocele in North China. However, this may affect the oxidative stress associated with homocysteine expression, which in turn affects semen parameters in VC patients. Larger studies are needed to validate our findings.

华北精索静脉曲张患者的 MTHFRC 677T 基因多态性与同型半胱氨酸
目的 探讨亚甲基四氢叶酸还原酶(MTHFR)C677T基因多态性、同型半胱氨酸与华北地区精索静脉曲张男性不育的相关性。研究人员招募了 100 名患有精索静脉曲张(VC;II-III 级,VC 组)的不育男性和 100 名精液指标正常且无精索静脉曲张的健康男性(NC 组),对他们进行 PCR 芯片、血液和精液检测。与 NC 组的 CC 基因型相比,NC 组的 TT 基因型和 VC 组的 CC 基因型在精子活力(P=0.191;P=0.130)、精子密度(P=0.591;P=0.643)、血浆同型半胱氨酸水平(P=0.511;P=0.677)和精浆 MDA(P=0.752;P=0.451)方面均无明显变化。相比之下,TT 基因型的精索静脉曲张患者血浆同型半胱氨酸水平和精浆 MDA 水平更高(P=0.511;P=0.677;P=0.451)。然而,这可能会影响与同型半胱氨酸表达相关的氧化应激,进而影响精索静脉曲张患者的精液参数。我们需要更大规模的研究来验证我们的发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Andrologia
Andrologia 医学-男科学
CiteScore
5.60
自引率
8.30%
发文量
292
审稿时长
6 months
期刊介绍: Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
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