Anatomical Science International最新文献

{"title":"Anatomical features of coexisting horseshoe kidney and double inferior vena cava.","authors":"Naofumi Horio, Narumi Miyaura, Kenta Nagahori, Daisuke Kiyoshima, Yoko Ueda, Masahito Yamamoto, Takashi Okazaki, Shogo Hayashi","doi":"10.1007/s12565-025-00860-3","DOIUrl":"https://doi.org/10.1007/s12565-025-00860-3","url":null,"abstract":"<p><p>To describe a rare case of coexisting horseshoe kidney (HSK) and double inferior vena cava (DIVC) observed during a cadaveric dissection course and to analyze the embryological and clinical implications of this anatomical variation. A detailed anatomical dissection of a 96-year-old Japanese male cadaver was performed during a gross anatomy course. The kidneys were fused by a fibrous and parenchymal isthmus below the inferior mesenteric artery. Four right renal arteries and three left renal arteries were identified, along with an additional renal vein arising from the isthmus and coursing to the left adrenal gland. The right and the left IVCs ascended bilaterally along the aorta, with no communicating veins observed. Histological findings revealed atrophic renal tubules in the isthmus, with increased connective tissue. This report highlights the high variability of renal and vascular anatomy in coexisting HSK and DIVC. Recognition of such variations is essential for radiologists and surgeons to avoid complications during renal transplantation, vascular surgery, and imaging interpretation. In addition, the kidneys and the IVC development occur simultaneously between weeks 4 and 8 of embryogenesis. Therefore, abnormalities of retroperitoneal structures that occur during embryological development can lead to variations.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical variants of the vertebral artery and their relationship with craniocervical disorders and surgical considerations: a systematic review and meta-analysis.","authors":"Juan José Valenzuela-Fuenzalida, Javiera Del Villar-Valdebenito, Lorenzo Aburto, Fernanda Berríos, Diego Nazar Izquierdo, Macarena Rodriguez- Luengo, Maja Czerniachowska, Pablo Nova Baeza, Mathias Orellana-Donoso, Alejandra Suazo-Santibañez, Alejandro Bruna-Mejias, Gustavo Oyanedel-Amaro, Heather F Smith, Juan Sanchis-Gimeno","doi":"10.1007/s12565-025-00855-0","DOIUrl":"10.1007/s12565-025-00855-0","url":null,"abstract":"<p><p>The vertebral artery (VA) supplies almost one-third of the blood flow to the brain, contributing mainly to its posterior circulation. This article provides a comprehensive overview of the different anatomical variations related to the origin, course, and termination of the VA and associated clinical implications. Data were compiled from numerous published studies accessed from the databases Medline, Scopus, Web of Science, Google Scholar, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and Literature in Health Sciences (LILACS) as of January 2024. Methodological quality was evaluated with an assurance tool for anatomical studies (AQUA). Pooled prevalence was estimated using a random effects model, and differences in VA variant rates were assessed. VA variants were identified that could be separated into three categories: variation in origin, course, and terminal branches. A total of 16 studies met the established selection criteria for the current meta-analysis. VA variations were reported at an overall prevalence of 11% (CI: 7%-15%) and a heterogeneity of 77%. Statistically significantly higher rates were revealed in the following subgroups of the sample: imaging examinations versus cadavers (p = 0.032); right side of the body versus left (p = 0.034); and bilaterally versus unilaterally (p = 0.019). Concerns listed in included studies primarily focused on the possibility of iatrogenic damage during surgical procedures. A few studies also indicated higher rates of VA variants in patients who reported symptoms of recurrent headaches, vertigo, dizziness, and/or syncope. The presence of VA variants is high and can occur in various regions. However, the most important clinical consideration is that individuals with this variant must be constantly monitored since their posterior cerebral circulation could be affected. To avoid iatrogenic damage to the VA, clinicians should employ medical imaging to evaluate its course and branches prior to surgical interventions in the region.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of systemic and regional anatomy on medical students' body donation willingness and the underlying factors.","authors":"Rui Gong, Nuo Xu, Ruofan Lin, Yile Gu, Yan Xia, Wenyan Yang, Zhihong Li, Yehui Lv","doi":"10.1007/s12565-025-00857-y","DOIUrl":"10.1007/s12565-025-00857-y","url":null,"abstract":"<p><p>Cadaveric anatomy remains indispensable in medical education. However, the development of body donation in China faces challenges due to various socio-cultural constraints. Although medical students constitute only a minor proportion of body and organ donors, their attitudes toward donation may reflect their perceptions of silent mentors and cognition of organ donation to some extent. The findings may help clarify the effects of anatomical education and even the humanistic spirit conveyed by instructors, while also providing guidance for optimizing anatomy curricula to cultivate compassion and professionalism in future physicians alongside anatomical expertise. This research endeavors to explore the impact of anatomical education, specifically systemic and regional anatomy, on medical students' propensity to donate their bodies posthumously, as well as the motivations driving this inclination. A randomized survey was conducted among 289 clinical medicine students at Shanghai University of Medicine and Health Sciences. The survey assessed students' willingness to donate their bodies and their association with anatomy learning experiences. Participation in systemic and regional anatomy education significantly increased students' willingness to donate their bodies. This enhancement was particularly pronounced among the group of students who received intensified \"curriculum ideological and political\" education (p ≤ 0.05). Notably, in contrast to observations among foreign medical students, Chinese students exhibited a sustained upward trend in donation willingness after anatomy learning. This divergence may be attributed to differences in religious beliefs and learning processes, as well as the incorporation of \"Curriculum Ideology and Politics\" within Chinese medical curriculum. This study suggests that reinforcing cadaveric anatomy experiences and addressing cultural and educational factors can promote body donation. By understanding the motivations and influences that shape students' donation decisions, medical educators and policymakers can develop targeted interventions to increase donation rates and ensure the availability of cadavers for medical education and research.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence regarding the coracobrachialis longus muscle: is it certain?","authors":"George Triantafyllou, Łukasz Olewnik, George Tsakotos, Maria Piagkou","doi":"10.1007/s12565-025-00856-z","DOIUrl":"https://doi.org/10.1007/s12565-025-00856-z","url":null,"abstract":"","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo flavoprotein functional imaging of the mouse auditory cortex combined with neuroanatomical tracing.","authors":"Tetsufumi Ito, Yosuke Hirayama","doi":"10.1007/s12565-025-00854-1","DOIUrl":"https://doi.org/10.1007/s12565-025-00854-1","url":null,"abstract":"<p><p>In vivo functional imaging is a powerful tool for identifying the functional organization of the cerebral cortex. Flavoprotein autofluorescence imaging detects the metabolic activity of endogenous flavoprotein in neuronal tissue and thus provides an indirect measure of brain activity. As flavoprotein imaging is minimally invasive and does not require expensive equipment or reagents, it is easily combined with tract tracing methods and serves as a valuable approach for investigating functional organization of the brain. This paper provides a step-by-step protocol for flavoprotein imaging to record cortical activity in response to auditory stimuli, along with a method for injecting neuroanatomical tracers into functionally identified cortical regions. Key factors for obtaining reproducible results include stabile head fixation, a controlled dark imaging environment, maintenance of body temperature, and oxygen saturation monitoring.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced fixation techniques for high-sensitivity molecular imaging: effectiveness of glyoxal fixation for immunostaining.","authors":"Kohtarou Konno, Miwako Yamasaki, Masahiko Watanabe","doi":"10.1007/s12565-025-00848-z","DOIUrl":"https://doi.org/10.1007/s12565-025-00848-z","url":null,"abstract":"<p><p>Chemical fixation is essential in immunohistochemistry for detecting molecular localization. The gold-standard fixative for immunohistochemistry is 4% paraformaldehyde (PFA); however, its properties-such as tissue shrinkage and protein cross-linking through methylene bridges-often restrict antibody access, posing challenges to achieving specific binding reactions. This is particularly true for receptors and ion channels condensed in the synaptic cleft, postsynaptic density, or trigger zone of action potentials. To overcome this problem, several laboratories have attempted antigen-exposure techniques. Recently, we demonstrated that fixation by glyoxal, a dialdehyde with two carbon atoms, enables specific detection of molecular groups that are difficult to be detected in PFA-fixed tissues. Here, we summarize the advantages and precautions in the use of glyoxal fixative.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditions of Excellence: neuroanatomy at the forefront of the new era.","authors":"Kathleen S Rockland","doi":"10.1007/s12565-025-00852-3","DOIUrl":"https://doi.org/10.1007/s12565-025-00852-3","url":null,"abstract":"<p><p>Neuroanatomy is emerging from decades of neglect, where the synthetic approach, a neuroanatomical hallmark, was too often, as an aspersion, dismissed as \"descriptive.\" The resurgence is in part driven by new technical advances (e.g., better visualization tools; larger sample sizes), many of which are briefly described here and treated in more detail elsewhere in this Special Issue. Another factor is the over-due recognition that a seemingly \"descriptive\" approach can be positively compatible with synthesis, integration, and conceptual formulation. The first two sections of this brief overview highlight advances in the level of microscopic visualization and the increasing availability of large data sets (\"big data\"). Illustrative examples of specific applications are drawn from the literature. A last section briefly discusses how neuroanatomy might be expected to develop further as the field continues to move forward.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ATAC-seq protocol for neuroscience research.","authors":"Nur Hasan Prince, Misako Hamamura, Yi Zhou, Yuki Fujita","doi":"10.1007/s12565-025-00850-5","DOIUrl":"https://doi.org/10.1007/s12565-025-00850-5","url":null,"abstract":"<p><p>ATAC-seq (assay for transposase-accessible chromatin using sequencing) enables high resolution mapping of chromatin accessibility, providing critical insights into gene regulatory mechanisms in neuroscience research. This protocol presents an optimized approach for applying ATAC-seq to neural tissues and cells, addressing the unique challenges of preserving chromatin integrity in these delicate samples. We detail the key steps of tissue processing, nuclear isolation, transposition reactions, and library preparation specifically adapted for neural applications. The method requires minimal starting material (20,000-50,000 cells) while maintaining high sensitivity for detecting regulatory elements involved in neural development, plasticity, and neurological disorders. This tailored protocol facilitates robust investigation of neuroepigenomic regulation in diverse experimental contexts.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing heart anatomy education in middle schools: virtual reality or tablet-based mobile applications?","authors":"Rabia Koca, Hamide Nur Çevik Özdemir","doi":"10.1007/s12565-025-00853-2","DOIUrl":"10.1007/s12565-025-00853-2","url":null,"abstract":"<p><p>This study examines the effects of virtual reality (VR) and tablet-based mobile applications (TBMA) in teaching heart anatomy to middle school students. A randomized-controlled trial was conducted in which 84 middle school students were divided into three groups: VR (n = 28), TBMA (n = 28), and control (n = 28). The students' knowledge levels regarding heart anatomy were assessed before and after the applications. In addition, the student's metacognitive awareness and satisfaction levels were measured after the TBMA and VR applications. The participants' opinions regarding the applications were evaluated using qualitative analysis techniques. Descriptive statistics, variance analysis, and t tests were used to analyze quantitative data, and Colaizzi's education method was prepared sevenfold to examine qualitative data. A significant increase in heart anatomy knowledge levels was observed in the distribution of VR and TBMA. However, no significant difference was found between the groups (p > 0.05). In addition, while the metacognitive awareness of heart anatomy was higher in the students in the VR group, the students in the TBMA group were more satisfied with the learning process. Students stated that learning heart anatomy with VR and TBMA methods was fun, informative, and enjoyable and that such applications should be used more in classes. This study reveals that technology-supported teaching methods can positively affect students' learning processes while teaching heart anatomy. More comprehensive research should be conducted with randomized-controlled and mixed-method studies in different age groups and various course subjects to evaluate the sustainability and effectiveness of technology-based applications.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling dynamics of nuclear pore and chromatin via HS-AFM.","authors":"Goro Nishide, Richard W Wong","doi":"10.1007/s12565-025-00849-y","DOIUrl":"https://doi.org/10.1007/s12565-025-00849-y","url":null,"abstract":"<p><p>High-speed atomic force microscopy (HS-AFM) enables real-time visualization of biological processes with nanometer-level resolution. This review highlights how HS-AFM has been instrumental in uncovering the dynamic interplay between nuclear pore complexes (NPCs)-which regulate nucleocytoplasmic transport-and genome guardians, including DNA repair proteins and chromatin regulators. Structurally, the NPCs resemble a multi-layered spider cobweb, serving as crucial molecular gatekeepers for maintaining cellular homeostasis, while genome guardians safeguard genomic integrity through DNA repair and chromatin organization. Through HS-AFM, the researchers have gained unprecedented insights into NPC dynamics, revealing their adaptability during nuclear transport, chromatin reorganization, and viral infection. It has also elucidated how genome guardians interact with NPCs, influencing chromatin organization at the nuclear periphery and regulating nucleocytoplasmic trafficking. These discoveries underscore the critical role of NPC-genome interactions in genome stability, gene expression, and nuclear transport, with broad implications for diseases such as cancer, viral infections, and neurodegenerative disorders. In conclusion, HS-AFM has transformed our ability to study the nuclear landscape at the nanoscale, bridging the gap between structural biology and functional genomics. By capturing the real-time molecular dynamics of NPCs and chromatin, HS-AFM provides an essential tool for unraveling the mechanisms that govern nuclear transport and genome regulation. Future advancements in HS-AFM technology, including higher temporal resolution, correlative imaging, and AI-driven analysis, will further expand its potential in biomedical research, paving the way for novel diagnostic and therapeutic strategies.</p>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}