结合神经解剖示踪的小鼠听觉皮层体内黄蛋白功能成像。

IF 1.2 4区 医学 Q3 ANATOMY & MORPHOLOGY
Tetsufumi Ito, Yosuke Hirayama
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引用次数: 0

摘要

体内功能成像是识别大脑皮层功能组织的有力工具。黄蛋白自身荧光成像检测内源性黄蛋白在神经元组织中的代谢活性,从而提供了脑活动的间接测量。由于黄蛋白成像是微创的,不需要昂贵的设备或试剂,它很容易与路追踪方法相结合,是研究大脑功能组织的一种有价值的方法。本文提供了一个循序渐进的方案,黄蛋白成像记录皮层活动对听觉刺激的反应,以及一种将神经解剖示踪剂注射到功能识别的皮层区域的方法。获得可重复性结果的关键因素包括稳定的头部固定、受控的黑暗成像环境、体温维持和血氧饱和度监测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vivo flavoprotein functional imaging of the mouse auditory cortex combined with neuroanatomical tracing.

In vivo functional imaging is a powerful tool for identifying the functional organization of the cerebral cortex. Flavoprotein autofluorescence imaging detects the metabolic activity of endogenous flavoprotein in neuronal tissue and thus provides an indirect measure of brain activity. As flavoprotein imaging is minimally invasive and does not require expensive equipment or reagents, it is easily combined with tract tracing methods and serves as a valuable approach for investigating functional organization of the brain. This paper provides a step-by-step protocol for flavoprotein imaging to record cortical activity in response to auditory stimuli, along with a method for injecting neuroanatomical tracers into functionally identified cortical regions. Key factors for obtaining reproducible results include stabile head fixation, a controlled dark imaging environment, maintenance of body temperature, and oxygen saturation monitoring.

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来源期刊
Anatomical Science International
Anatomical Science International 医学-解剖学与形态学
CiteScore
2.80
自引率
8.30%
发文量
50
审稿时长
>12 weeks
期刊介绍: The official English journal of the Japanese Association of Anatomists, Anatomical Science International (formerly titled Kaibogaku Zasshi) publishes original research articles dealing with morphological sciences. Coverage in the journal includes molecular, cellular, histological and gross anatomical studies on humans and on normal and experimental animals, as well as functional morphological, biochemical, physiological and behavioral studies if they include morphological analysis.
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