Anatomical Science International最新文献

Blood-brain barrier-penetrant AAV vectors for cell type-specific gene expression in the mouse brain. 血脑屏障渗透AAV载体在小鼠脑细胞类型特异性基因表达。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-23 DOI: 10.1007/s12565-025-00899-2

Hirokazu Hirai, Shu Iwazaki, Reona Hoshino, Tokuzo Maruyama, Ryo Aoki, Putri T Radhiyanti, Ayumu Konno

引用次数: 0

The synaptic organization of the human temporal lobe neocortex by high-resolution transmission, focused ion beam scanning, and electron microscopic tomography. 通过高分辨率传输、聚焦离子束扫描和电子显微断层扫描研究人类颞叶新皮层的突触组织。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-23 DOI: 10.1007/s12565-025-00900-y

Astrid Rollenhagen, Joachim H R Lübke

{"title":"The synaptic organization of the human temporal lobe neocortex by high-resolution transmission, focused ion beam scanning, and electron microscopic tomography.","authors":"Astrid Rollenhagen, Joachim H R Lübke","doi":"10.1007/s12565-025-00900-y","DOIUrl":"https://doi.org/10.1007/s12565-025-00900-y","url":null,"abstract":"Fine-scale transmission electron microscopy (TEM), focused ion beam scanning EM (FIB), and EM tomography have opened a new window on the synaptic organization of the normal, developing and pathologically altered brain in experimental animals. Progress in the human brain has been slower, due to technical challenges and the problem of tissue availability from donors that underwent epilepsy or tumor surgery. The present manuscript is in part an overview of the geometry of synaptic boutons in surgical biopsy samples taken from human temporal lobe neocortex ('hTLN'). Here, the number, size, and shape of active zones (the equivalent of functional neurotransmitter release sites) and the three functionally defined pools of synaptic vesicles were quantified, with comparisons to the same parameters in experimental animals. High-resolution TEM tomography further allowed new insights concerning the readily releasable pool of synaptic vesicles, one of the key structural elements in synaptic transmission and plasticity. The quantitative 3D models of synaptic boutons provide the basis for numerical and/or Monte Carlo simulations of various signal cascades underlying synaptic transmission that at least in humans are still only partially accessible for experiment. In a second focus, we provide a step-by-step walk-through with illustrations of basic methodology for tissue preparation and analysis, for both TEM and FIB-SEM, including a thorough discussion of the main advantages and disadvantages of the several techniques and the particular challenge of working with human tissue.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare combined variations of the axillary artery: superficial ulnar artery and circumflex humeral arteries. 罕见的腋窝动脉联合变异：尺浅动脉和旋肱动脉。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-14 DOI: 10.1007/s12565-025-00897-4

Ryota Takegoshi, Kota Yanagisawa, Yoko Takeshita, Kouta Honda, Sakuramaru Tanaka, Hiroshi Isome, Yoko Ueda, Kenta Nagahori, Daisuke Kiyoshima, Zhe-Wu Jin, Takashi Okazaki, Masahito Yamamoto, Hayato Terayama, Kaori Suyama, Shogo Hayashi

{"title":"Rare combined variations of the axillary artery: superficial ulnar artery and circumflex humeral arteries.","authors":"Ryota Takegoshi, Kota Yanagisawa, Yoko Takeshita, Kouta Honda, Sakuramaru Tanaka, Hiroshi Isome, Yoko Ueda, Kenta Nagahori, Daisuke Kiyoshima, Zhe-Wu Jin, Takashi Okazaki, Masahito Yamamoto, Hayato Terayama, Kaori Suyama, Shogo Hayashi","doi":"10.1007/s12565-025-00897-4","DOIUrl":"https://doi.org/10.1007/s12565-025-00897-4","url":null,"abstract":"The variations in the branching pattern of the axillary artery are clinically significant. We report an extremely rare combination of arterial variations discovered during the routine anatomical dissection of the left upper limb in a 73-year-old male cadaver. The most significant findings were a superficial ulnar artery originating directly from the axillary artery, proximal to the ansa pectoralis. The superficial ulnar artery coursed superficially in the forearm and terminated as the primary contribution to the superficial palmar arch. Additionally, a common trunk for the circumflex scapular and thoracodorsal arteries arose directly from the axillary artery. Finally, the anterior circumflex humeral artery and posterior circumflex humeral artery arose from a common trunk originating from the deep brachial artery. The posterior circumflex humeral artery passed through the triangular interval bordered by the teres major, long head of the triceps brachii, and humerus. A common trunk for the circumflex scapular and thoracodorsal arteries arose directly from the axillary artery, separate from the subscapular artery. The superficial course of the superficial ulnar artery and the atypical posterior circumflex humeral artery course increase the risk of iatrogenic injury during shoulder and arm surgeries.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive classification and depth analysis of the transverse facial artery based on cadaveric and radiological evidence. 基于尸体和放射学证据的面横动脉的综合分类和深度分析。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-05 DOI: 10.1007/s12565-025-00898-3

Fatma Ok, Burak Karip, Fulya Temizsoy Korkmaz, Papatya Keleş, Tuba Selçuk Can, Mazhar Yalçın

{"title":"A comprehensive classification and depth analysis of the transverse facial artery based on cadaveric and radiological evidence.","authors":"Fatma Ok, Burak Karip, Fulya Temizsoy Korkmaz, Papatya Keleş, Tuba Selçuk Can, Mazhar Yalçın","doi":"10.1007/s12565-025-00898-3","DOIUrl":"https://doi.org/10.1007/s12565-025-00898-3","url":null,"abstract":"The transverse facial artery is a key vascular structure supplying the lateral face and is critically important in surgical procedures such as facelifts, facial trauma repair, and injectable treatments. However, detailed anatomical studies on the transverse facial artery remain scarce. This study aimed to comprehensively evaluate the anatomical variations, depth, branching patterns and clinical significance of the transverse facial artery (TFA) using both cadaveric dissection and computed tomography angiography (CTA). This study employed a dual-modality design, combining cadaveric dissection of 40 hemifaces and computed tomography angiography of 180 hemifaces. Parameters analyzed included the artery's origin, course, length, depth from the skin surface, and branching pattern. The transverse facial artery was classified according to its origin, branching, and topographic course. In cadaveric specimens, a single transverse facial artery was present in 87.5% of cases, most commonly originating from the superficial temporal artery (90%) and less frequently from the external carotid artery (5%). Imaging revealed a single artery in 91.1% of cases, with 83.3% originating from the superficial temporal artery, 3.9% directly from the external carotid artery, and 12.8% from its bifurcation. Six branching types and three course-based zones were defined, with the superior zone being the most frequent (65%). The artery was significantly deeper in the inferior zone (p < 0.05). In this study, we report for the first time the measurement of the depth of the TFA from the skin surface, with a mean value of 10.54 mm. This comprehensive anatomical and morphometric evaluation provides clinically relevant insights into transverse facial artery variability. The findings offer a refined classification and practical guidance to support safer surgical and aesthetic procedures.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

For celebrating a complete digital transformation of ASI. 庆祝ASI的全面数字化转型。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-05 DOI: 10.1007/s12565-025-00896-5

Hayato Ohshima

引用次数: 0

Rare anatomical variation: analysis of an aberrant right subclavian artery in three cadavers. 罕见的解剖变异：三具尸体右锁骨下动脉异常分析。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-04 DOI: 10.1007/s12565-025-00895-6

Yosuke Fujii, Yuki Katoh, Hiroki Yokota, Hiroyuki Hara, Tomonori Harada, Kazuyuki Shimada, Shuichi Hirai

{"title":"Rare anatomical variation: analysis of an aberrant right subclavian artery in three cadavers.","authors":"Yosuke Fujii, Yuki Katoh, Hiroki Yokota, Hiroyuki Hara, Tomonori Harada, Kazuyuki Shimada, Shuichi Hirai","doi":"10.1007/s12565-025-00895-6","DOIUrl":"https://doi.org/10.1007/s12565-025-00895-6","url":null,"abstract":"An aberrant right subclavian artery (ARSA) is a congenital vascular anomaly in which the right subclavian artery originates directly from the aortic arch distal to the left subclavian artery. Although often asymptomatic, ARSA can lead to clinical complications, such as dysphagia, upper respiratory issues, and vascular events. In this study, we examined the gross anatomical and histological characteristics of the ARSA based on three cadavers selected from a total of 7 ARSA cases identified among 3,158 specimens dissected between 1948 and 2024 at Nihon University School of Medicine (overall incidence: 0.22%). All three cases exhibited a retroesophageal course of the ARSA and absence of the right recurrent laryngeal nerve, replaced by a non-recurrent laryngeal nerve. One case also presented a bicarotid trunk. Histological analysis of one cadaver revealed variable patterns of intimal thickening in the ARSA, particularly at the flattened segment compressed between the esophagus and vertebral body, suggesting localized hemodynamic stress. Despite these structural changes, the tunica media remained intact. No Kommerell diverticulum was observed. These findings highlight the embryological and structural basis of ARSA and its potential clinical relevance in surgical and endovascular procedures. The presence of ARSA should prompt careful preoperative planning to avoid nerve injury or procedural complications. The observed correlation between arterial flattening and histological changes may also help explain some embolic phenomena previously reported in patients with ARSA. This study contributes new insights into the anatomical variations and histopathological features of ARSA, with particular relevance to vascular and head and neck surgery.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Anatomical relationship between the vinculum breve derived from the flexor digitorum superficialis tendon and the volar plate in the proximal interphalangeal joint of the hand: variation in the distribution of attachments. 纠正：手近端指间关节指浅屈肌腱衍生的短静脉与掌侧板的解剖关系：附着物分布的变化。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-26 DOI: 10.1007/s12565-025-00894-7

Takeo Ichigaya, Keiko Fujita, Tomohiro Kurisaki, Kazuhiro Takano, Masabumi Nagashima

引用次数: 0

Historical trends in neuroanatomical tract-tracing techniques. 神经解剖束示踪技术的历史趋势。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-24 DOI: 10.1007/s12565-025-00892-9

Yasushi Kobayashi, Toshiyasu Matsui, Kiyomasa Nishii

{"title":"Historical trends in neuroanatomical tract-tracing techniques.","authors":"Yasushi Kobayashi, Toshiyasu Matsui, Kiyomasa Nishii","doi":"10.1007/s12565-025-00892-9","DOIUrl":"https://doi.org/10.1007/s12565-025-00892-9","url":null,"abstract":"Numerous neuroanatomical tract-tracing techniques have been reported to demonstrate the origin, course, and termination of neural pathways. New techniques have been developed to achieve higher specificity and efficiency. Early tract-tracing studies at the microscopic level used non-specific staining, for example, by tracing fiber bundles of normal nervous tissue using myelin staining. However, when combined with neurodevelopment or degeneration, myelin staining provides important information regarding the major pathways, even in the early years. Impregnation techniques, including the Golgi method, have contributed to the demonstration of connections between individual neurons. Specific staining for degenerating myelin and axons has established most of the basic knowledge of the major pathways described in classical neuroanatomical textbooks. Since the 1970s, tract-tracing techniques using axonal transport have opened a new era of more detailed and selective connectivity analyses. They show normal morphology of neural pathways, including synaptic contact with target cells. Various tracer substances have been reported that can be injected into the nervous tissue and transported anterogradely or retrogradely through axons. Neurotropic viruses that can be transported trans-synaptically are particularly useful for analyzing the chains of neuronal connections. Introducing genes encoding tracer substances or reporters using various techniques, including electroporation, lipofection, and viral vectors, can yield higher intracellular concentrations of these molecules and provide high-contrast images of the entire dendritic tree and axonal arborization of labeled neurons. Since gene manipulation allows us not only to visualize neurons but also to control their functions, we can now conduct integrative research on neuronal morphology and function.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complicated architecture of cortical microcircuit: a comprehensive review. 皮层微电路的复杂结构综述。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-20 DOI: 10.1007/s12565-025-00877-8

Toshio Miyashita

{"title":"Complicated architecture of cortical microcircuit: a comprehensive review.","authors":"Toshio Miyashita","doi":"10.1007/s12565-025-00877-8","DOIUrl":"https://doi.org/10.1007/s12565-025-00877-8","url":null,"abstract":"Since Mountcastle's discovery of vertically oriented cells responding to a particular sensory modality, the radially aligned columnar organization has been extensively studied. The concept of columnar organization in the cerebral cortex has been accepted as a fundamental principle. Anatomically, different types of columns are recognized, which vary in size, components, organization, and across species. However, the precise definition of a column and its role as a functional unit remains debatable. As the brain functions as a network, understanding how columnar organization is integrated within cortical circuits is essential, but many aspects of its integration into cortical circuits remain elusive despite considerable investigation. In the cerebral cortex, connected pyramidal neuron pairs often share common synaptic inputs. This fine-scale specificity of synaptic connections within cortical neurons creates subnetworks that are thought to be embedded within functional columns. However, how to embed into the functional column and what type of columnar structure or unidentified structure to embed in a subnetwork remain largely unexplored. This review comprehensively introduces both anatomically and physiologically identified functional units and discusses potential approaches for integrating these concepts.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single cell labeling combined with electrophysiological identification. 单细胞标记与电生理鉴定相结合。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-12 DOI: 10.1007/s12565-025-00882-x

Takahiro Furuta

引用次数: 0