Anatomical Science International最新文献_第2页

Single-cell endogenous protein labeling via CRISPR-Cas9-mediated genome editing in the mouse brain. 通过crispr - cas9介导的基因组编辑在小鼠大脑中进行单细胞内源性蛋白标记。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-07-14 DOI: 10.1007/s12565-025-00866-x

Motokazu Uchigashima, Takayasu Mikuni

{"title":"Single-cell endogenous protein labeling via CRISPR-Cas9-mediated genome editing in the mouse brain.","authors":"Motokazu Uchigashima, Takayasu Mikuni","doi":"10.1007/s12565-025-00866-x","DOIUrl":"https://doi.org/10.1007/s12565-025-00866-x","url":null,"abstract":"High-precision mapping of endogenous proteins is essential for understanding the molecular mechanism underlying neuronal functions in the brain. The SLENDR (single-cell labeling of endogenous proteins by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9-mediated homology-directed repair) technique provides single-cell endogenous protein labeling with genetically encoded tags within the mammalian brain through precise genome editing via homology-directed repair (HDR). This technique is based on the introduction of HDR-mediated genome editing into neuronal progenitors in embryonic brains by in utero electroporation. Subsequent histological analyses enable high-resolution interrogation of the subcellular distribution of endogenous proteins within a single neuron using conventional fluorescent microscopy. Here, we describe a step-by-step protocol for the SLENDR technique to label endogenous proteins with genetically encoded tags in single pyramidal cells of the mouse primary somatosensory cortex. This protocol would be helpful to visualize the molecular organization underlying biological processes at single-neuron levels in the brain, such as signal processing from synaptic inputs to neuronal outputs across different scales.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlative light and electron microscopy analysis for neurobiological applications. 相关光学和电子显微镜分析在神经生物学中的应用。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-07-09 DOI: 10.1007/s12565-025-00868-9

Kazunori Toida, Keita Satoh, Haruyo Yamanishi, Yukari Minami-Ogawa, Simone N T Kurial, Emi Kiyokage

引用次数: 0

Acceleration of axonal regeneration by GABA/Gly excitation. GABA/Gly刺激加速轴突再生。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-07-09 DOI: 10.1007/s12565-025-00869-8

Chitoshi Takayama, Tsukasa Yafuso

引用次数: 0

ht-MASH: a high-throughput, cost-effective, and robust protocol for microscopic 3D imaging of human angio- and cytoarchitecture in large human brain samples. ht-MASH：一种高通量、高成本效益和强大的方案，用于在大型人脑样本中对人类血管和细胞结构进行显微3D成像。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-07-08 DOI: 10.1007/s12565-025-00859-w

Sven Hildebrand, Johannes Franz, Shubharthi Sengupta, Anna Schueth, Andreas Herrler, Alard Roebroeck

{"title":"ht-MASH: a high-throughput, cost-effective, and robust protocol for microscopic 3D imaging of human angio- and cytoarchitecture in large human brain samples.","authors":"Sven Hildebrand, Johannes Franz, Shubharthi Sengupta, Anna Schueth, Andreas Herrler, Alard Roebroeck","doi":"10.1007/s12565-025-00859-w","DOIUrl":"https://doi.org/10.1007/s12565-025-00859-w","url":null,"abstract":"The performance of many optical tissue clearing protocols has considerably improved in the last few years, so that now even notoriously difficult specimen such as highly myelinated human brain tissue can be rendered highly transparent. However, optical tissue clearing is still routinely performed on relatively small samples, especially in the case of the human brain. Recent advances in histological tissue processing now allow scaling up the clearing process considerably towards much larger samples. Yet so far, these methods can have considerable drawbacks in their feasibility to be implemented routinely, especially in smaller laboratories. Here, we present an updated version of our MASH protocol, which allows optical tissue clearing of very large human brain tissue samples and labelling of angio- and cytoarchitecture therein. This pipeline is cost-efficient and easy to implement, so that even smaller labs can apply it at scale. At the same time, the use of rapid prototyping using 3D printing to create custom clearing equipment is versatile enough to be adjusted to other optical tissue clearing methods than the one used in this study (e.g., aqueous methods such as CUBIC or other solvent-based methods of the DISCO family), sample sizes or tissue types. Our pipeline has, therefore, the potential to advance optical tissue clearing and labelling of large human tissue samples towards a more robust and routine implementation in the blooming field of 3D histology.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracing of specific neural pathways in the rat brain using lentiviral vectors for retrograde gene transfer. 用慢病毒载体逆行基因转移追踪大鼠脑内的特定神经通路。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-07-01 DOI: 10.1007/s12565-025-00864-z

Saaya Akama, Yoshio Iguchi, Shigeki Kato, Kazuto Kobayashi

{"title":"Tracing of specific neural pathways in the rat brain using lentiviral vectors for retrograde gene transfer.","authors":"Saaya Akama, Yoshio Iguchi, Shigeki Kato, Kazuto Kobayashi","doi":"10.1007/s12565-025-00864-z","DOIUrl":"https://doi.org/10.1007/s12565-025-00864-z","url":null,"abstract":"Understanding the structural and the functional organization of neural circuits in the brain is a fundamental goal of neuroscience. Lentiviral vectors for retrograde gene transfer transduce neurons through the entry from synaptic terminals and enable tracing and manipulation of neuronal populations of interests based on the synaptic connections. The highly efficient and neuron-specific retrograde gene transfer (NeuRet) vectors are derived from the pseudotyping of human immunodeficiency virus type 1-based vectors with fusion envelope glycoproteins. Viral RNA genome is reverse-transcribed and synthesized DNA is integrated into the host genomes, allowing stable and long-term expression of transgenes. Dorsal striatum, the input layer of the basal ganglia, integrates signals from various brain regions, including cerebral cortex, thalamus, and ventral midbrain, and plays a critical role in motor control, learning, and decision-making. Consequently, understanding the input and the output connectivity of the dorsal striatum is fundamental to revealing how circuits within the basal ganglia contribute to physiological and behavioral functions, and its impairments are related to neurological disorders. This paper outlines the procedures for injecting a NeuRet vector carrying a green fluorescent protein gene into the sub-regions of dorsal striatum in rats, followed by immunohistochemistry to detect the transgene expression in the brain.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the enchanted loom: an interpretation of reality considering epistemology. 重访魔法织机：考虑到认识论的现实解释。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-06-30 DOI: 10.1007/s12565-025-00861-2

Sén Takeda

引用次数: 0

Visualization of nanostructures in neuronal growth cones and axons using super-resolution structured illumination microscopy. 利用超分辨率结构照明显微镜观察神经元生长锥和轴突的纳米结构。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-06-30 DOI: 10.1007/s12565-025-00863-0

Motohiro Nozumi, Michihiro Igarashi

引用次数: 0

Combination method of macroscopic imaging with MRI and microscopic imaging with super resolution microscopy for brain microstructure analysis. MRI宏观成像与超分辨显微镜显微成像相结合的脑显微结构分析方法。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-06-30 DOI: 10.1007/s12565-025-00862-1

Yuka Nakano, Kenji F Tanaka, Yoshifumi Abe

{"title":"Combination method of macroscopic imaging with MRI and microscopic imaging with super resolution microscopy for brain microstructure analysis.","authors":"Yuka Nakano, Kenji F Tanaka, Yoshifumi Abe","doi":"10.1007/s12565-025-00862-1","DOIUrl":"https://doi.org/10.1007/s12565-025-00862-1","url":null,"abstract":"Identifying disease-relevant and therapy-related brain regions remains a major challenge in studies using animal models of psychiatric disorders. Conventional hypothesis-driven approaches often result in limited or subjective identification of brain regions. In this study, we propose an integrative method combining unbiased whole-brain structural magnetic resonance imaging (MRI) screening and detailed histological analysis. Our methodology uses structural MRI to systematically detect volumetric changes across the entire brain, enabling the identification of target regions without relying on predefined hypotheses. Once brain regions are identified, super-resolution microscopy (SRM) is employed to determine microstructural alterations at the cellular level, focusing on neurons and glial cells within those regions. To exemplify the utility of this method, we applied it to a mouse model treated with electroconvulsive therapy (ECT), an intervention which is known to increase hippocampal volume. Our demonstration highlights the potential of this approach to systematically search for brain regions of interest, providing valuable insights and guiding future studies toward a more focused exploration of key aspects of psychiatric disorder research, both in terms of pathophysiology and therapeutic action.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reflections on "Enhancing heart anatomy education in middle schools": integrating artificial intelligence, educational equity, and instructional coherence. “加强中学心脏解剖教育”的思考：整合人工智能、教育公平、教学连贯。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-06-30 DOI: 10.1007/s12565-025-00865-y

Betül Digilli Ayaş

{"title":"Reflections on \"Enhancing heart anatomy education in middle schools\": integrating artificial intelligence, educational equity, and instructional coherence.","authors":"Betül Digilli Ayaş","doi":"10.1007/s12565-025-00865-y","DOIUrl":"https://doi.org/10.1007/s12565-025-00865-y","url":null,"abstract":"The integration of digital technologies into anatomy education continues to evolve, with virtual reality (VR) and mobile applications gaining traction in enhancing student engagement and conceptual understanding. Building upon the study by Koca and Çevik Özdemir (2025), which compares the effects of VR and tablet based tools on middle school students' learning of cardiac anatomy, this commentary explores additional dimensions that could further enrich the educational landscape, particularly the role of artificial intelligence (AI) in promoting instructional coherence and inclusivity. The commentary highlights a variety of AI-driven tools that have been established in the anatomy education literature, including adaptive learning platforms, large language models like ChatGPT, three-dimensional visualization environments, and digital cadaver systems such as the Anatomage Table. Moreover, the role of intelligent tutoring systems (e.g., Smart Tutor, Why2-Atlas), NLP-based platforms (e.g., IBM Watson Tutor, Ada), and AI-supported feedback systems is discussed in relation to their capacity to personalize learning experiences and enhance accessibility. Special attention is also given to pedagogical equity, addressing how AI can support students with diverse learning needs by dynamically tailoring instructional content. By synthesizing current findings and technological advancements, this commentary advocates for a more integrative approach to digital anatomy instruction, one that merges immersive technologies with responsive AI systems to foster deeper understanding, learner autonomy, and broader educational inclusion.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic tools for imaging microcirculation via plasma labeling. 通过血浆标记成像微循环的遗传工具。

IF 1.2 4区医学

Anatomical Science International Pub Date : 2025-06-27 DOI: 10.1007/s12565-025-00858-x

Xiaowen Wang, Marta Vittani, Ashley Bomin Lee, Philip Gade Knak, Hajime Hirase

引用次数: 0