Anatomical Science International最新文献_第2页

A detailed protocol for sparse and bright labeling of neurons via dual adeno-associated virus vectors with Cre recombination. 通过双腺相关病毒载体与Cre重组对神经元进行稀疏和明亮标记的详细方案。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-08 DOI: 10.1007/s12565-025-00867-w

Megumu Takahashi, Hidenobu Mizuno, Masato Koike, Takuji Iwasato, Hiroyuki Hioki

{"title":"A detailed protocol for sparse and bright labeling of neurons via dual adeno-associated virus vectors with Cre recombination.","authors":"Megumu Takahashi, Hidenobu Mizuno, Masato Koike, Takuji Iwasato, Hiroyuki Hioki","doi":"10.1007/s12565-025-00867-w","DOIUrl":"https://doi.org/10.1007/s12565-025-00867-w","url":null,"abstract":"Adeno-associated virus (AAV) vectors are widely used to label individual neurons by expressing fluorescent proteins. To analyze neuronal morphology in detail, it is important to achieve sparse and strong expression of fluorescent proteins for clearly visualizing cell bodies, dendrites, and axons. However, injections of high-titer AAV vectors often result in excessive cell labeling, while injecting low-titer AAV vectors reduces signal intensity. Here, we provide a detailed, step-by-step protocol for achieving sparse and bright neuronal labeling using dual AAV vectors with Cre recombination, based on the Supernova system. We prepared two types of AAV vectors: one is a driver vector (AAV2/1-SYN-iCre-BGHpA) that expresses Cre recombinase under a neuron-specific promoter, and the other is a reporter vector (AAV2/1-SynTetOff FLEX-GFP) that encodes GFP with a flip-excision switch (FLEX) and the Tet-Off system. When a mixture of a serially diluted reporter vector and a high-titer driver vector was stereotaxically injected into the mouse brain, the number of labeled neurons and the fluorescence intensity of GFP decreased in a dose-dependent manner. Conversely, diluting the driver vector decreased the number of GFP-expressing cells while the fluorescence intensity of GFP remained high. Furthermore, this approach proved effective for systemic delivery via retro-orbital injection of AAV2/B10 vectors. This protocol includes comprehensive reagent lists, stepwise injection procedures, and troubleshooting tips, allowing researchers to reproducibly achieve sparse and bright labeling through both stereotaxic and retro-orbital injections.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Introduction to the CLEM technique developed in the field of neuroanatomy. 更正：介绍在神经解剖学领域发展起来的CLEM技术。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-07 DOI: 10.1007/s12565-025-00893-8

Takaichi Fukuda

引用次数: 0

Internal carotid artery agenesis: a systematic review of prevalence, basal brain circulation variations, and associated pathologies. 颈内动脉发育不全：患病率、基础脑循环变化和相关病理的系统综述。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-06 DOI: 10.1007/s12565-025-00873-y

Mathias Orellana-Donoso, Juan Sanchis-Gimeno, Sary Afandi-Rebolledo, Pablo Nova-Baeza, Paloma García-Mena, Alejandra Suazo-Santibañez, Rocío Peñailillo-Ibarra, Iván Valdés-Orrego, Juan José Valenzuela-Fuenzalida

{"title":"Internal carotid artery agenesis: a systematic review of prevalence, basal brain circulation variations, and associated pathologies.","authors":"Mathias Orellana-Donoso, Juan Sanchis-Gimeno, Sary Afandi-Rebolledo, Pablo Nova-Baeza, Paloma García-Mena, Alejandra Suazo-Santibañez, Rocío Peñailillo-Ibarra, Iván Valdés-Orrego, Juan José Valenzuela-Fuenzalida","doi":"10.1007/s12565-025-00873-y","DOIUrl":"https://doi.org/10.1007/s12565-025-00873-y","url":null,"abstract":"The internal carotid artery (ICA) is a crucial vessel, and its congenital absence, known as ICA agenesis, is a rare vascular anomaly. This systematic review aimed to provide a comprehensive overview of the available evidence on this condition. We systematically searched multiple databases from inception until January 2024. Two authors independently screened titles and abstracts, and a third reviewer was involved if consensus could not be reached. Data extraction and quality assessment were performed using validated the AQUA tool and the JBI critical appraisal checklist tool. A total of 138 (7.8%) articles out of 1,778 met the inclusion criteria, reporting 2040 subjects from which 176 presented (8.6%) ICA agenesis. Most of the studies were case reports (96%), and the included studies originated from various countries, with the United States (17.39%), Japan (14.49%), Turkey (10.87%), and India (10.14%) contributing the most. Left-sided agenesis was the most common (67.39%), followed by right-sided (42.03%) and bilateral (22.46%) agenesis. The most reported clinical manifestations were Horner's syndrome, increased risk of intracranial aneurysms, and pituitary gland abnormalities. ICA agenesis is a rare vascular anomaly with a left-sided predominance. While some patients may develop associated clinical conditions, many remain asymptomatic due to the development of robust collateral circulation. Comprehensive preoperative evaluation and awareness of these anatomical variations are crucial to guide surgical planning and minimize the risk of complications. PROSPERO registration number: CRD42024592673.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct imaging of the three-dimensional ultrastructure of neuronal organelles. 神经元细胞器三维超微结构的直接成像。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-05 DOI: 10.1007/s12565-025-00888-5

Daisuke Koga, Ryosuke Morinaga, Satoshi Kusumi

{"title":"Direct imaging of the three-dimensional ultrastructure of neuronal organelles.","authors":"Daisuke Koga, Ryosuke Morinaga, Satoshi Kusumi","doi":"10.1007/s12565-025-00888-5","DOIUrl":"https://doi.org/10.1007/s12565-025-00888-5","url":null,"abstract":"In the context of cell morphological analyses observing organelles embedded within the cell matrix is difficult. The osmium maceration method is a unique technique used to directly observe the three-dimensional structure of organelles through scanning electron microscopy, without requiring time-consuming and labor-intensive reconstruction. In this method, tissues are immersed in a diluted osmium solution for several days to remove cytosolic soluble proteins and filamentous structures, including microfilaments, intermediate filaments, and microtubules, from the freeze-cracked surfaces of cells, leaving the subcellular structures, Golgi apparatus, mitochondria, and smooth and rough endoplasmic reticulum intact. Specimen preparation involves several key steps, specifically pre-fixation with aldehyde fixatives, tissue excision, trimming, post-fixation with osmium tetroxide solution, dimethyl sulfoxide cracking (i.e., freeze-cracking), the thawing of cracked tissues, osmium maceration, osmium fixation, conductive staining (tannin-osmium method), dehydration, drying, mounting, metal coating, and scanning electron microscopy observations. Here, we present a step-by-step protocol based on the maceration method using neural cells as an example, ensuring reproducibility and consistent results for neurons and various other cell types. Moreover, the results presented indicate that the osmium maceration method is effective for elucidating the three-dimensional intracellular ultrastructure of neurons.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hippocampal architecture viewed through the eyes of methodological development. 从方法论发展的角度看海马体结构。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-05 DOI: 10.1007/s12565-025-00878-7

Ling Zhao, Nicola Palomero-Gallagher

引用次数: 0

Correction: A systematic review of the use of human body donor models for postgraduate laparoscopic surgical training. 更正：对研究生腹腔镜手术训练中使用人体供体模型的系统回顾。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-04 DOI: 10.1007/s12565-025-00889-4

Sedat Alp Pinar, Joseph J Morrow, Chia Yew Kong

引用次数: 0

Seven (quadruple right and triple left) renal arteries and their topological relationship with other branches of the abdominal aorta. 七根肾动脉（四根右动脉和三根左动脉）及其与腹主动脉其他分支的拓扑关系。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-02 DOI: 10.1007/s12565-025-00890-x

Shigeyuki Esumi, Yoshikazu Koba, Yoshihiro Kumagai, Yoko Nishitani, Takaichi Fukuda

{"title":"Seven (quadruple right and triple left) renal arteries and their topological relationship with other branches of the abdominal aorta.","authors":"Shigeyuki Esumi, Yoshikazu Koba, Yoshihiro Kumagai, Yoko Nishitani, Takaichi Fukuda","doi":"10.1007/s12565-025-00890-x","DOIUrl":"https://doi.org/10.1007/s12565-025-00890-x","url":null,"abstract":"Supernumerary renal arteries are frequently observed, but their developmental origin remains controversial. One hypothesis, the ladder theory, ascribed origins of multiple renal arteries to a segmental array of mesonephric arteries. However, recent studies in both rodents and humans demonstrated evidence against this theory. We describe a rare variation in a Japanese female cadaver exhibiting seven renal arteries encountered during student dissection practice in 2023. On the right side, the main renal artery branched off from the aorta 29 mm below the origin of the superior mesenteric artery. Three additional renal arteries branched off at the level of or below the inferior mesenteric artery. On the left side, two renal arteries branched off at usual high positions, but another branch below the inferior mesenteric artery. In 43 cadavers dissected in 2023, all renal arteries including duplicate type, but except for the present case, arose at high positions near the superior mesenteric artery. Incidence of connection between multiple renal arteries and adrenal and/or gonadal arteries was 78.6% (11 out of 14 kidneys). Importantly, all these connections were observed at high-positioned branches. Combining present results with previous data in literatures revealed the close relationship between high-positional branching and connections with adrenal/gonadal arteries (p = 0.0056), suggesting two factors influencing renal artery development: (1) prenatal, interconnected arteries at high positions, and (2) low-positioned individual branches arising from the aorta during kidney ascent with poor interconnectivity. Arteries involved in these two events might regress with various degrees, leading to diversity in number and connectivity of renal arteries.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic resonance angiography-based assessment of the renal artery and its branching pattern. 基于磁共振血管造影的肾动脉及其分支形态评估。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-01 DOI: 10.1007/s12565-025-00891-w

Necmi Keskin, Belgin Bamac, Ozgur Cakir, Tuncay Colak, Cagatay Barut

{"title":"Magnetic resonance angiography-based assessment of the renal artery and its branching pattern.","authors":"Necmi Keskin, Belgin Bamac, Ozgur Cakir, Tuncay Colak, Cagatay Barut","doi":"10.1007/s12565-025-00891-w","DOIUrl":"https://doi.org/10.1007/s12565-025-00891-w","url":null,"abstract":"This study aimed to investigate the detailed anatomy of the renal arteries through magnetic resonance angiography (MRA), offering an alternative to conventional imaging techniques. By identifying the variations, spatial orientation, and vertebral origin of the renal arteries, the study seeks to provide comprehensive anatomical data that may support surgeons and interventional radiologists in clinical planning and decision-making. Magnetic resonance angiography images of 196 individuals who underwent abdominal imaging were retrospectively retrieved from the Picture Archiving and Communication System (PACS). The analysis focused on the vertebral level of renal artery origin, angle of emergence, distances from major abdominal aortic branches, and renal artery branching patterns. These parameters were statistically assessed based on age and sex using SPSS 25. In male individuals, the distances between the renal arteries and both the inferior mesenteric artery and the aortic bifurcation were significantly greater than in females (p < 0.05). A statistically significant positive correlation was observed between age and the coronal angle of the left renal artery in both sexes and in the overall group (p = 0.001). The most frequent origin point of the right renal artery was identified at the mid-level between the L1 and L2 vertebrae, whereas the left renal artery commonly originated at the upper level of L2 in both sexes. The anatomical parameters defined by age and sex in this study may improve diagnostic accuracy for clinicians. These findings can aid interventional radiologists in catheter selection and procedure planning, supporting more personalized and effective interventions.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric analysis of mental foramen in retained cadaveric specimens of mandibles of Sri Lankan population. 斯里兰卡人下颌骨尸体标本颏孔形态计量学分析。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-07-31 DOI: 10.1007/s12565-025-00883-w

Dadallage Tharanga De Silva, Usliyanage Clifford Priyantha Perera

{"title":"Morphometric analysis of mental foramen in retained cadaveric specimens of mandibles of Sri Lankan population.","authors":"Dadallage Tharanga De Silva, Usliyanage Clifford Priyantha Perera","doi":"10.1007/s12565-025-00883-w","DOIUrl":"https://doi.org/10.1007/s12565-025-00883-w","url":null,"abstract":"Understanding the variation of the location of the mental foramen in the mandible will aid in the prevention of injury to associated structures during invasive procedures in the mental area. This study was carried out using 228 mental foramina in 114 dry mandibles belonging to the adult Sri Lankan population of the southern region of Sri Lanka to determine the shape, size, direction, existence of accessory foramina, and location with respect to anatomical landmarks. A single mental foramen was the most common in Sri Lankan adults, and 7% of them had accessory mental foramen on either side. The most common characteristic of the mental foramen was an oval shape, located below the 2nd premolar tooth in the posterior-superior direction. The mean distance from the mandibular midline to the mental foramen was 25.98 ± 1.9 mm and 25.64 ± 2.02 mm on the right and left sides, respectively. This study offers substantial data about possible variations in physical characteristics and the location of the mental foramen in a population in the southern region of Sri Lanka. Hence, it is possible to use the relative position of teeth or distances from various borders of the mandible to precisely locate the mental foramen. Therefore, this information is crucial for clinicians, who must be aware of these variances and the necessity of determining the precise location of the mental foramen before doing surgery in this region.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geometric relationships of neural and bony landmarks of the cranial base. 颅底神经和骨标记的几何关系。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-07-30 DOI: 10.1007/s12565-025-00886-7

Jonathan A Millard, Kaivon Kouhestani, Amanda Swaak, Alexandra Luna, Elina M Baltins, Chelsea J Bengson, Benjamin Mann, Sally S Greenberg, Ryan Baukhages

{"title":"Geometric relationships of neural and bony landmarks of the cranial base.","authors":"Jonathan A Millard, Kaivon Kouhestani, Amanda Swaak, Alexandra Luna, Elina M Baltins, Chelsea J Bengson, Benjamin Mann, Sally S Greenberg, Ryan Baukhages","doi":"10.1007/s12565-025-00886-7","DOIUrl":"https://doi.org/10.1007/s12565-025-00886-7","url":null,"abstract":"The skull base serves as structural support for the brain and as a conduit for neurovasculature. Traditional morphometric methods have intrinsic limitations which make wholistic assessment of this anatomy challenging. Here we applied geometric morphometric techniques to address the problems associated with traditional morphometric strategies for evaluating skeletal and soft tissue components of the skull base. A Microscribe® i+ 3-D digitizer was used to register the location of 20 3-D skeletal and neural landmarks on the skull base of 80 anatomic body donors (mean age 83.0 ± 10.1 years). Linear measurements of neurocranial dimensions were collected with traditional methods. A principal component analysis (PCA) was applied to Procrustes aligned coordinates of the 3-D data. The first principal component accounted for 34.24% of the variation and was associated with changes in clival width and sella turcica length. Shape changes associated with PC1 scores correlated with configuration size (centroid size) (R2 = .532, p < .001). PC2 (12.85%) was associated with isolated changes in clival width driven by the jugular tubercles. Results related to PC3 (11.10%) showed variation in skull base flattening. PC2 and PC3 results were correlated with neurocranial length and measurements of cranial width. Skull base size emerged as a principal driver of skull base shape. The jugular tubercle and surrounding foramina represented another locale of variation. Finally, our results support the idea that a wide range of non-pathologic skull base flattening may exist, as this shape axis was a major contributor to the variation seen in the sample.","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0