{"title":"Heinrich Ewald Hering’s discovery of the heart pacemaker: Hering, Tawara and Aschoff’s search for its morphological basis, the sinoatrial node, and why they failed","authors":"Marcos C. De Almeida","doi":"10.1007/s12565-023-00711-z","DOIUrl":"10.1007/s12565-023-00711-z","url":null,"abstract":"<div><p>Two groups of investigators investigated the heart pacemaker and its morphological basis in the early twentieth century. The first group was formed by Henrich Ewald Hering (physiologist), Sunao Tawara and Ludwig Aschoff (morphologists). The second group was composed of James Mackenzie (general practitioner and clinical investigator), Arthur Keith and Martin Flack (morphologists). These groups were formed almost at the same time in 1903. Their work resulted in the discovery of the atrioventricular node and Purkinje network (Sunao Tawara, in 1906), heart pacemaker (H E Hering, in 1907) and sinoatrial node (Keith and Flack, in 1907). Here, it is shown how the interconnections of the concurrent works of these groups resulted in the discovery not only of the function, but also of the structure of the sinoatrial node.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10244279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Muscle–tendon arrangement and intramuscular nerve distribution of flexor digitorum superficialis in the siamang (Symphalangus syndactylus), western lowland gorilla (Gorilla gorilla gorilla), western chimpanzee (Pan troglodytes verus), and Japanese macaque (Macaca fuscata)","authors":"Kenji Emura, Eishi Hirasaki, Takamitsu Arakawa","doi":"10.1007/s12565-023-00713-x","DOIUrl":"10.1007/s12565-023-00713-x","url":null,"abstract":"<div><p>Flexor digitorum superficialis (FDS) shows diverse muscle–tendon arrangements among primates. The intramuscular nerve distribution pattern is a criterion for discussing the homology of FDS. In this study, the muscle–tendon arrangement and intramuscular nerve distribution of FDS were examined in the siamang, western lowland gorilla, western chimpanzee, and Japanese macaques. The FDS had muscle bellies to digits II–V. FDS had proximal belly and intermediate tendon except for siamang. Distal belly to digit II (in the western lowland gorilla and western chimpanzees) or distal bellies to digits II and V (in Japanese macaque) originated from the intermediate tendon. In all specimens, nerve branches within digit III belly extended into digit IV belly, and nerve branch(es) within digit IV belly extended into digit V belly. This consistent pattern suggested that each muscle belly to digits III–V is interspecifically homologous. The digit II belly in the siamang and the distal belly to digit II in the western lowland gorilla, western chimpanzees, and Japanese macaques could be homologous based on their similar innervating patterns. The proximal belly was innervated by branches from the communicating nerve between median and ulnar nerves in the western lowland gorilla or branches from median and ulnar nerves in western chimpanzees. In the siamang and Japanese macaque, the whole FDS was innervated by median nerve. The proximal belly in the western lowland gorilla, western chimpanzees, and Japanese macaques could be classified into different groups from the other part of the FDS.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9862647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan J. Cardona, Arada Chaiyamoon, Ana Carrera, Francisco Reina, Joe Iwanaga, R. Shane Tubbs
{"title":"Penetration of the facial nerve by the posterior auricular artery: case report, comprehensive review and clinical–surgical applications","authors":"Juan J. Cardona, Arada Chaiyamoon, Ana Carrera, Francisco Reina, Joe Iwanaga, R. Shane Tubbs","doi":"10.1007/s12565-023-00708-8","DOIUrl":"10.1007/s12565-023-00708-8","url":null,"abstract":"<div><p>Anatomical variations between the facial nerve and adjacent arteries are rare. However, knowledge of such anatomical variations is important to the surgeon who operates on or near the facial nerve. Herein, we report an unusual finding between the extracranial part of the facial nerve and a nearby artery. During routine dissection of the right facial nerve trunk, the posterior auricular artery was found to pierce the nerve effectively forming a nerve loop. The nerve was pierced by the artery soon after its exit from the stylomastoid foramen. This case is detailed and a review on this topic presented, specifically identifying previously reported studies describing this or similar variations, and the relationship between the posterior auricular artery and facial nerve trunk in general. Piercing of the facial nerve trunk by the posterior auricular artery appears to be rare. However, such a relationship should be known by the clinician who treats patients with pathologies of the facial nerve trunk. To our knowledge, this is the first report of this variation in an adult. Due to such rarity, this case is of archival value for those who might describe it or similar cases in the future.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-023-00708-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9858094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Novel combination method of wide-range serial sectioning and 3D reconstruction visualizing both macro-level dynamics and micro-level interactions in an attempt to analyze the female pelvic floor","authors":"Satoru Muro, Keiichi Akita","doi":"10.1007/s12565-023-00710-0","DOIUrl":"10.1007/s12565-023-00710-0","url":null,"abstract":"<div><p>The present report presents details of the method for combining wide-range serial sectioning and 3D reconstruction using an adult cadaver. For several decades, anatomists have utilized a variety of non-destructive three-dimensional (3D) visualization methods to complement gross anatomical analysis methods. These include vascular casting for the visualization of vascular morphology and micro-CT for the visualization of bone morphology. However, these conventional methods are restricted by the properties and sizes of the target structures. Here, we introduce a method to conduct 3D reconstruction based on wide-range serial histological sections from adult cadavers, which overcomes previous restrictions. An attempt at 3D visualization of the female pelvic floor muscles provides a detailed description of the procedure. Supplemental video and 3D PDF files allow multifaceted observation of 3D images. Wide-range serial sectioning visualizes morphology beyond the scope of conventional methods, while 3D reconstruction enables non-destructive 3D visualization of any structure that can be observed on a histological section, including skeletal muscle, smooth muscle, ligaments, cartilage, connective tissue, blood vessels, nerves, lymph nodes, and glands. The novel combination of both methods is instrumental in meso-anatomy, a discipline intermediate between macro-anatomy and micro-anatomy.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-023-00710-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9602038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Keiko Fukino, Kohsei Iida, Masahiro Tsutsumi, Joe Iwanaga, Keiichi Akita
{"title":"Evaluation of morphological features of palatopharyngeus insertion into the thyroid cartilage","authors":"Keiko Fukino, Kohsei Iida, Masahiro Tsutsumi, Joe Iwanaga, Keiichi Akita","doi":"10.1007/s12565-023-00709-7","DOIUrl":"10.1007/s12565-023-00709-7","url":null,"abstract":"<div><p>The attachment of the palatopharyngeus extended from the posterior end of the thyroid cartilage to the posterior margin of the inferior constrictor attachment that might contribute to successive swallowing movements. Laryngeal elevation is essential for proper swallowing and breathing. Recently, clinical research has demonstrated that the palatopharyngeus, a longitudinal muscle of the pharynx, is involved in the elevation of the larynx. However, the morphological relationship between the larynx and palatopharyngeus remains unclear. In the present study, we analyzed the attachment site and characteristics of the palatopharyngeus in the thyroid cartilage. We evaluated 14 halves of seven heads from Japanese cadavers (average age: 76.4 years); 12 halves, anatomically and two halves histologically. A part of the palatopharyngeus, which originated from the inferior aspect of the palatine aponeurosis, was attached to the inner and outer surfaces of the thyroid cartilage through collagen fibers. The attachment area extends from the posterior end of the thyroid cartilage to the posterior margin of the attachment site of the inferior constrictor. The palatopharyngeus may elevate the larynx with the suprahyoid muscles and contribute to successive movements of swallowing with surrounding muscles. Based on our findings and previous studies, palatopharyngeus with various muscle bundle directions may be essential for the coordination of continuous swallowing events.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9657912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kateřina Vymazalová, Omar Šerý, Petr Králík, Radka Dziedzinská, Zuzana Musilová, Jan Frišhons, Tomáš Vojtíšek, Marek Joukal
{"title":"Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection","authors":"Kateřina Vymazalová, Omar Šerý, Petr Králík, Radka Dziedzinská, Zuzana Musilová, Jan Frišhons, Tomáš Vojtíšek, Marek Joukal","doi":"10.1007/s12565-023-00707-9","DOIUrl":"10.1007/s12565-023-00707-9","url":null,"abstract":"<div><p>With the onset of the COVID-19 pandemic, a problem arose with classic body donation programmes for obtaining cadavers for anatomical dissections, science and research. The question has emerged whether bodies of individuals who died of COVID-19 or were infected by SARS-CoV-2 could be admitted to Departments of Anatomy. To determine the risk of SARS-CoV-2 transmission to employees or students, the presence and stability of SARS-CoV-2 RNA in cadavers after fixation agents’ application and subsequent post-fixation baths over time were examined. The presence of viral RNA in swabs from selected tissues was assessed by the standardized routine RNA isolation protocol and subsequent real-time PCR analysis. To support the results obtained from the tissue swabs, samples of RNA were exposed in vitro to short and long-term exposure to the components of the injection and fixation solutions used for the bodies’ conservation. Substantial removal of SARS-CoV-2 RNA was observed in post-mortem tissue following perfusion with 3.5% phenol, 2.2% formaldehyde, 11.8% glycerol and 55% ethanol, and subsequent post-fixation in an ethanol bath. In vitro experiments showed significant effects of formaldehyde on SARS-CoV-2 RNA, while phenol and ethanol showed only negligible effects. We conclude that cadavers subjected to fixation protocols as described here should not pose a considerable risk of SARS-CoV-2 infection while being handled by students and staff and are, therefore, suitable for routine anatomical dissections and teaching.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-023-00707-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9971591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science","authors":"Yuki Tajika, Nobukazu Ishii, Yoshihiro Morimura, Kyosuke Fukuda, Mitsuaki Shikada, Tohru Murakami, Sotaro Ichinose, Yuhei Yoshimoto, Hirohide Iwasaki","doi":"10.1007/s12565-023-00705-x","DOIUrl":"10.1007/s12565-023-00705-x","url":null,"abstract":"<div><p>Correlative microscopy and block-face imaging (CoMBI) is an imaging method, which is characterized by the ability to obtain both serial block-face images as a 3-dimentional (3D) dataset and sections for 2-dimentional (2D) light microscopic analysis. These 3D and 2D morphological data can be correlated with each other to facilitate data interpretation. CoMBI is an easy-to-install and low-cost 3D imaging method since its system can be assembled by the researcher using a regular microtome, consumer digital camera, and some self-made devices, and its installation and instruction manuals are open-source. After the first release of CoMBI method from our laboratory, CoMBI systems have been installed in more than a dozen laboratories and are used for 3D analysis of various biological specimens. Typical application of CoMBI is 3D anatomical analysis using the natural color and contrast of the specimen. We have been using CoMBI for analyzing human brain to obtain the fine 3D anatomy as a reference to determine the causes of neurological diseases and to improve the effectiveness of surgery. Recently, we have been using CoMBI for detecting the colors of chromogens, which are used for labeling specific molecules. Mouse embryos colored with X-gal, a conventional chromogen for detecting LacZ products, were imaged using CoMBI, and the 3D distribution of X-gal was successfully visualized. Thus, CoMBI can now be used for many purposes, including 3D anatomical analysis, 2D microscopy using sections, and 3D distribution of specific molecules. These suggest that CoMBI should be more widely used in the field of biological research.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9607785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy","authors":"Shinji Urata, Shigeo Okabe","doi":"10.1007/s12565-023-00703-z","DOIUrl":"10.1007/s12565-023-00703-z","url":null,"abstract":"<div><p>The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1<sup>+/GFP</sup> mice. This study’s method was effective in elucidating inner ear function under both physiological and pathological conditions.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-023-00703-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9607746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexander M. Kerner, Andrea J. Grisold, Freyja-Maria Smolle-Jüttner, Niels Hammer
{"title":"Publisher Correction: Incidental finding of Clostridium perfringens on human corpses used for the anatomy course","authors":"Alexander M. Kerner, Andrea J. Grisold, Freyja-Maria Smolle-Jüttner, Niels Hammer","doi":"10.1007/s12565-023-00704-y","DOIUrl":"10.1007/s12565-023-00704-y","url":null,"abstract":"","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-023-00704-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9966505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emily Deem, Nicholas Passalacqua, Diana Messer, Paige Sanseverino, Katie Zejdlik, Alexandra Klales
{"title":"A review of anatomical terminology for the hip bone","authors":"Emily Deem, Nicholas Passalacqua, Diana Messer, Paige Sanseverino, Katie Zejdlik, Alexandra Klales","doi":"10.1007/s12565-023-00702-0","DOIUrl":"10.1007/s12565-023-00702-0","url":null,"abstract":"<div><p>Anatomists have been attempting to standardize anatomical terminology of the human body and in doing so created the <i>Terminologia Anatomica</i> as a standard language of anatomy. Despite developments such as the <i>Terminologia Anatomica</i>, a lack of consistency of anatomical terminology is still seen across and within disciplines. This study explores the variation in terminology for the hip bone through a literature review. In total, ten terms used to describe the hip bone were searched in PubMed, JSTOR, and EBSCO databases: “coxal bone,” “hip bone,” “innominate bone,” “<i>os coxa</i>,” “<i>os coxae</i>,” “<i>ossa coxa</i>,” “<i>ossa coxae</i>,” “<i>os innominatum</i>,” “<i>os pelvicum</i>,” and “pelvic bone.” Results found that the terms “hip bone” and “pelvic bone” were the most commonly used terms, with the most frequently used discipline being medicine. However, we argue against the use of these terms in favor of “coxal bone” or “innominate bone.” “Hip bone” should be avoided due to its colloquial nature, and “pelvic bone” is not specific enough to the structure of the hip. Latin terms are often incorrectly conjugated (e.g., “<i>os coxae</i>”); therefore, the English equivalent should be used when possible. Regardless, standardization of anatomical terminology should be adopted through consensus by practitioners, researchers, and stakeholders.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-023-00702-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}