Anatomical Science International最新文献_第9页

Rare anatomical variation: analysis of an aberrant right subclavian artery in three cadavers 罕见的解剖变异：三具尸体右锁骨下动脉异常分析。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-09-04 DOI: 10.1007/s12565-025-00895-6

Yosuke Fujii, Yuki Katoh, Hiroki Yokota, Hiroyuki Hara, Tomonori Harada, Kazuyuki Shimada, Shuichi Hirai

{"title":"Rare anatomical variation: analysis of an aberrant right subclavian artery in three cadavers","authors":"Yosuke Fujii, Yuki Katoh, Hiroki Yokota, Hiroyuki Hara, Tomonori Harada, Kazuyuki Shimada, Shuichi Hirai","doi":"10.1007/s12565-025-00895-6","DOIUrl":"10.1007/s12565-025-00895-6","url":null,"abstract":"<div><p>An aberrant right subclavian artery (ARSA) is a congenital vascular anomaly in which the right subclavian artery originates directly from the aortic arch distal to the left subclavian artery. Although often asymptomatic, ARSA can lead to clinical complications, such as dysphagia, upper respiratory issues, and vascular events. In this study, we examined the gross anatomical and histological characteristics of the ARSA based on three cadavers selected from a total of 7 ARSA cases identified among 3,158 specimens dissected between 1948 and 2024 at Nihon University School of Medicine (overall incidence: 0.22%). All three cases exhibited a retroesophageal course of the ARSA and absence of the right recurrent laryngeal nerve, replaced by a non-recurrent laryngeal nerve. One case also presented a bicarotid trunk. Histological analysis of one cadaver revealed variable patterns of intimal thickening in the ARSA, particularly at the flattened segment compressed between the esophagus and vertebral body, suggesting localized hemodynamic stress. Despite these structural changes, the tunica media remained intact. No Kommerell diverticulum was observed. These findings highlight the embryological and structural basis of ARSA and its potential clinical relevance in surgical and endovascular procedures. The presence of ARSA should prompt careful preoperative planning to avoid nerve injury or procedural complications. The observed correlation between arterial flattening and histological changes may also help explain some embolic phenomena previously reported in patients with ARSA. This study contributes new insights into the anatomical variations and histopathological features of ARSA, with particular relevance to vascular and head and neck surgery.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":"101 4","pages":"449 - 457"},"PeriodicalIF":1.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Anatomical relationship between the vinculum breve derived from the flexor digitorum superficialis tendon and the volar plate in the proximal interphalangeal joint of the hand: variation in the distribution of attachments 纠正：手近端指间关节指浅屈肌腱衍生的短静脉与掌侧板的解剖关系：附着物分布的变化。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-26 DOI: 10.1007/s12565-025-00894-7

Takeo Ichigaya, Keiko Fujita, Tomohiro Kurisaki, Kazuhiro Takano, Masabumi Nagashima

引用次数: 0

Historical trends in neuroanatomical tract-tracing techniques 神经解剖束示踪技术的历史趋势。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-24 DOI: 10.1007/s12565-025-00892-9

Yasushi Kobayashi, Toshiyasu Matsui, Kiyomasa Nishii

{"title":"Historical trends in neuroanatomical tract-tracing techniques","authors":"Yasushi Kobayashi, Toshiyasu Matsui, Kiyomasa Nishii","doi":"10.1007/s12565-025-00892-9","DOIUrl":"10.1007/s12565-025-00892-9","url":null,"abstract":"<div><p>Numerous neuroanatomical tract-tracing techniques have been reported to demonstrate the origin, course, and termination of neural pathways. New techniques have been developed to achieve higher specificity and efficiency. Early tract-tracing studies at the microscopic level used non-specific staining, for example, by tracing fiber bundles of normal nervous tissue using myelin staining. However, when combined with neurodevelopment or degeneration, myelin staining provides important information regarding the major pathways, even in the early years. Impregnation techniques, including the Golgi method, have contributed to the demonstration of connections between individual neurons. Specific staining for degenerating myelin and axons has established most of the basic knowledge of the major pathways described in classical neuroanatomical textbooks. Since the 1970s, tract-tracing techniques using axonal transport have opened a new era of more detailed and selective connectivity analyses. They show normal morphology of neural pathways, including synaptic contact with target cells. Various tracer substances have been reported that can be injected into the nervous tissue and transported anterogradely or retrogradely through axons. Neurotropic viruses that can be transported trans-synaptically are particularly useful for analyzing the chains of neuronal connections. Introducing genes encoding tracer substances or reporters using various techniques, including electroporation, lipofection, and viral vectors, can yield higher intracellular concentrations of these molecules and provide high-contrast images of the entire dendritic tree and axonal arborization of labeled neurons. Since gene manipulation allows us not only to visualize neurons but also to control their functions, we can now conduct integrative research on neuronal morphology and function.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":"100 :","pages":"400 - 412"},"PeriodicalIF":1.7,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-025-00892-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complicated architecture of cortical microcircuit: a comprehensive review 皮层微电路的复杂结构综述。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-20 DOI: 10.1007/s12565-025-00877-8

Toshio Miyashita

{"title":"Complicated architecture of cortical microcircuit: a comprehensive review","authors":"Toshio Miyashita","doi":"10.1007/s12565-025-00877-8","DOIUrl":"10.1007/s12565-025-00877-8","url":null,"abstract":"<div><p>Since Mountcastle’s discovery of vertically oriented cells responding to a particular sensory modality, the radially aligned columnar organization has been extensively studied. The concept of columnar organization in the cerebral cortex has been accepted as a fundamental principle. Anatomically, different types of columns are recognized, which vary in size, components, organization, and across species. However, the precise definition of a column and its role as a functional unit remains debatable. As the brain functions as a network, understanding how columnar organization is integrated within cortical circuits is essential, but many aspects of its integration into cortical circuits remain elusive despite considerable investigation. In the cerebral cortex, connected pyramidal neuron pairs often share common synaptic inputs. This fine-scale specificity of synaptic connections within cortical neurons creates subnetworks that are thought to be embedded within functional columns. However, how to embed into the functional column and what type of columnar structure or unidentified structure to embed in a subnetwork remain largely unexplored. This review comprehensively introduces both anatomically and physiologically identified functional units and discusses potential approaches for integrating these concepts.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":"100 :","pages":"614 - 634"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single cell labeling combined with electrophysiological identification 单细胞标记与电生理鉴定相结合。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-12 DOI: 10.1007/s12565-025-00882-x

Takahiro Furuta

引用次数: 0

A detailed protocol for sparse and bright labeling of neurons via dual adeno-associated virus vectors with Cre recombination 通过双腺相关病毒载体与Cre重组对神经元进行稀疏和明亮标记的详细方案。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-08 DOI: 10.1007/s12565-025-00867-w

Megumu Takahashi, Hidenobu Mizuno, Masato Koike, Takuji Iwasato, Hiroyuki Hioki

{"title":"A detailed protocol for sparse and bright labeling of neurons via dual adeno-associated virus vectors with Cre recombination","authors":"Megumu Takahashi, Hidenobu Mizuno, Masato Koike, Takuji Iwasato, Hiroyuki Hioki","doi":"10.1007/s12565-025-00867-w","DOIUrl":"10.1007/s12565-025-00867-w","url":null,"abstract":"<div><p>Adeno-associated virus (AAV) vectors are widely used to label individual neurons by expressing fluorescent proteins. To analyze neuronal morphology in detail, it is important to achieve sparse and strong expression of fluorescent proteins for clearly visualizing cell bodies, dendrites, and axons. However, injections of high-titer AAV vectors often result in excessive cell labeling, while injecting low-titer AAV vectors reduces signal intensity. Here, we provide a detailed, step-by-step protocol for achieving sparse and bright neuronal labeling using dual AAV vectors with Cre recombination, based on the Supernova system. We prepared two types of AAV vectors: one is a driver vector (AAV2/1-SYN-iCre-BGHpA) that expresses Cre recombinase under a neuron-specific promoter, and the other is a reporter vector (AAV2/1-SynTetOff FLEX-GFP) that encodes GFP with a flip-excision switch (FLEX) and the Tet-Off system. When a mixture of a serially diluted reporter vector and a high-titer driver vector was stereotaxically injected into the mouse brain, the number of labeled neurons and the fluorescence intensity of GFP decreased in a dose-dependent manner. Conversely, diluting the driver vector decreased the number of GFP-expressing cells while the fluorescence intensity of GFP remained high. Furthermore, this approach proved effective for systemic delivery via retro-orbital injection of AAV2/B10 vectors. This protocol includes comprehensive reagent lists, stepwise injection procedures, and troubleshooting tips, allowing researchers to reproducibly achieve sparse and bright labeling through both stereotaxic and retro-orbital injections.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":"100 :","pages":"433 - 444"},"PeriodicalIF":1.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Introduction to the CLEM technique developed in the field of neuroanatomy 更正：介绍在神经解剖学领域发展起来的CLEM技术。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-07 DOI: 10.1007/s12565-025-00893-8

Takaichi Fukuda

引用次数: 0

Internal carotid artery agenesis: a systematic review of prevalence, basal brain circulation variations, and associated pathologies 颈内动脉发育不全：患病率、基础脑循环变化和相关病理的系统综述。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-06 DOI: 10.1007/s12565-025-00873-y

Mathias Orellana-Donoso, Juan Sanchis-Gimeno, Sary Afandi-Rebolledo, Pablo Nova-Baeza, Paloma García-Mena, Alejandra Suazo-Santibañez, Rocío Peñailillo-Ibarra, Iván Valdés-Orrego, Juan José Valenzuela-Fuenzalida

{"title":"Internal carotid artery agenesis: a systematic review of prevalence, basal brain circulation variations, and associated pathologies","authors":"Mathias Orellana-Donoso, Juan Sanchis-Gimeno, Sary Afandi-Rebolledo, Pablo Nova-Baeza, Paloma García-Mena, Alejandra Suazo-Santibañez, Rocío Peñailillo-Ibarra, Iván Valdés-Orrego, Juan José Valenzuela-Fuenzalida","doi":"10.1007/s12565-025-00873-y","DOIUrl":"10.1007/s12565-025-00873-y","url":null,"abstract":"<div><p>The internal carotid artery (ICA) is a crucial vessel, and its congenital absence, known as ICA agenesis, is a rare vascular anomaly. This systematic review aimed to provide a comprehensive overview of the available evidence on this condition. We systematically searched multiple databases from inception until January 2024. Two authors independently screened titles and abstracts, and a third reviewer was involved if consensus could not be reached. Data extraction and quality assessment were performed using validated the AQUA tool and the JBI critical appraisal checklist tool. A total of 138 (7.8%) articles out of 1,778 met the inclusion criteria, reporting 2040 subjects from which 176 presented (8.6%) ICA agenesis. Most of the studies were case reports (96%), and the included studies originated from various countries, with the United States (17.39%), Japan (14.49%), Turkey (10.87%), and India (10.14%) contributing the most. Left-sided agenesis was the most common (67.39%), followed by right-sided (42.03%) and bilateral (22.46%) agenesis. The most reported clinical manifestations were Horner’s syndrome, increased risk of intracranial aneurysms, and pituitary gland abnormalities. ICA agenesis is a rare vascular anomaly with a left-sided predominance. While some patients may develop associated clinical conditions, many remain asymptomatic due to the development of robust collateral circulation. Comprehensive preoperative evaluation and awareness of these anatomical variations are crucial to guide surgical planning and minimize the risk of complications. PROSPERO registration number: CRD42024592673.</p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":"101 3","pages":"268 - 304"},"PeriodicalIF":1.7,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct imaging of the three-dimensional ultrastructure of neuronal organelles 神经元细胞器三维超微结构的直接成像。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-05 DOI: 10.1007/s12565-025-00888-5

Daisuke Koga, Ryosuke Morinaga, Satoshi Kusumi

{"title":"Direct imaging of the three-dimensional ultrastructure of neuronal organelles","authors":"Daisuke Koga, Ryosuke Morinaga, Satoshi Kusumi","doi":"10.1007/s12565-025-00888-5","DOIUrl":"10.1007/s12565-025-00888-5","url":null,"abstract":"<div><p>In the context of cell morphological analyses observing organelles embedded within the cell matrix is difficult. The osmium maceration method is a unique technique used to directly observe the three-dimensional structure of organelles through scanning electron microscopy, without requiring time-consuming and labor-intensive reconstruction. In this method, tissues are immersed in a diluted osmium solution for several days to remove cytosolic soluble proteins and filamentous structures, including microfilaments, intermediate filaments, and microtubules, from the freeze-cracked surfaces of cells, leaving the subcellular structures, Golgi apparatus, mitochondria, and smooth and rough endoplasmic reticulum intact. Specimen preparation involves several key steps, specifically pre-fixation with aldehyde fixatives, tissue excision, trimming, post-fixation with osmium tetroxide solution, dimethyl sulfoxide cracking (i.e., freeze-cracking), the thawing of cracked tissues, osmium maceration, osmium fixation, conductive staining (tannin–osmium method), dehydration, drying, mounting, metal coating, and scanning electron microscopy observations. Here, we present a step-by-step protocol based on the maceration method using neural cells as an example, ensuring reproducibility and consistent results for neurons and various other cell types. Moreover, the results presented indicate that the osmium maceration method is effective for elucidating the three-dimensional intracellular ultrastructure of neurons. </p></div>","PeriodicalId":7816,"journal":{"name":"Anatomical Science International","volume":"100 :","pages":"598 - 613"},"PeriodicalIF":1.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12565-025-00888-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hippocampal architecture viewed through the eyes of methodological development 从方法论发展的角度看海马体结构。

IF 1.7 4区医学

Anatomical Science International Pub Date : 2025-08-05 DOI: 10.1007/s12565-025-00878-7

Ling Zhao, Nicola Palomero-Gallagher

引用次数: 0