Amino Acids最新文献

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Alterations of amino acids in older adults with Alzheimer’s Disease and Vascular Dementia 老年阿尔茨海默病和血管性痴呆患者氨基酸的改变
IF 3 3区 生物学
Amino Acids Pub Date : 2025-01-18 DOI: 10.1007/s00726-024-03442-1
Xin Ma, Xin-Meng Wang, Guo-Zhang Tang, Yi Wang, Xue‑Chun Liu, Shuai-Deng Wang, Peng Peng, Xiu-Hong Qi, Xin-Ya Qin, Yue‑Ju Wang, Chen-Wei Wang, Jiang-Ning Zhou
{"title":"Alterations of amino acids in older adults with Alzheimer’s Disease and Vascular Dementia","authors":"Xin Ma,&nbsp;Xin-Meng Wang,&nbsp;Guo-Zhang Tang,&nbsp;Yi Wang,&nbsp;Xue‑Chun Liu,&nbsp;Shuai-Deng Wang,&nbsp;Peng Peng,&nbsp;Xiu-Hong Qi,&nbsp;Xin-Ya Qin,&nbsp;Yue‑Ju Wang,&nbsp;Chen-Wei Wang,&nbsp;Jiang-Ning Zhou","doi":"10.1007/s00726-024-03442-1","DOIUrl":"10.1007/s00726-024-03442-1","url":null,"abstract":"<div><p>Metabolomics provide a promising tool for understanding dementia pathogenesis and identifying novel biomarkers. This study aimed to identify amino acid biomarkers for Alzheimer’s Disease (AD) and Vascular Dementia (VD). By amino acid metabolomics, the concentrations of amino acids were determined in the serum of AD and VD patients as well as age-matched healthy controls. Several differences in the concentration of amino acids were observed in AD patients compared to both healthy controls and VD patients. However, no significant distinction was found between healthy controls and VD patients. Considering comorbidities, cystine levels were higher in AD than in VD among non-diabetic patients, but not in those with diabetes. Notably, creatine, spermidine, cystine, and tyrosine demonstrated favorable results in decision curve analyses and good discriminative performances, suggesting their potential for clinical application. These fundings give novel perspectives of serum amino acids for predicting metabolic pathways in AD and VD pathogenesis.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03442-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus 桔梗赖氨酸丙二酸酰化的系统定性蛋白质组分析。
IF 3 3区 生物学
Amino Acids Pub Date : 2025-01-15 DOI: 10.1007/s00726-024-03432-3
Qingshan Yang, Shaowei Xu, Weimin Jiang, Fei Meng, Shuting Wang, Zongping Sun, Na Chen, Daiyin Peng, Juan Liu, Shihai Xing
{"title":"Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus","authors":"Qingshan Yang,&nbsp;Shaowei Xu,&nbsp;Weimin Jiang,&nbsp;Fei Meng,&nbsp;Shuting Wang,&nbsp;Zongping Sun,&nbsp;Na Chen,&nbsp;Daiyin Peng,&nbsp;Juan Liu,&nbsp;Shihai Xing","doi":"10.1007/s00726-024-03432-3","DOIUrl":"10.1007/s00726-024-03432-3","url":null,"abstract":"<div><p>In recent years, it was found that lysine malonylation modification can affect biological metabolism and play an important role in plant life activities. <i>Platycodon grandiflorus</i>, an economic crop and medicinal plant, had no reports on malonylation in the related literature. This study qualitatively introduces lysine malonylation in <i>P. grandiflorus</i>. A total of 888 lysine malonylation-modified proteins in <i>P. grandiflorus</i> were identified, with a total of 1755 modification sites. According to the functional annotation, malonylated proteins were closely related to catalysis, binding, and other reactions. Subcellular localization showed that related proteins were enriched in chloroplasts, cytoplasm, and nuclei, indicating that this modification could regulate various metabolic processes. Motif analysis showed the enrichment of Alanine (A), Cysteine (C), Glycine (G), and Valine (V) amino acids surrounding malonylated lysine residues. Metabolic pathway and protein-protein interaction network analyses suggested these modifications are mainly involved in plant photosynthesis. Moreover, malonylated proteins are also involved in stress and defense responses. This study shows that lysine malonylation can affect a variety of biological processes and metabolic pathways, and the contents are reported for the first time in <i>P. grandiflorus</i>, which can provide important information for further research on <i>P. grandiflorus</i> and lysine malonylation’s role in environment stress, photosynthesis, and secondary metabolites enrichment.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03432-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The relationship between dietary branched-chain and aromatic amino acids with the regulation of leptin and FTO genes in adipose tissue of patients undergoing abdominal surgery 膳食支链和芳香氨基酸与腹部手术患者脂肪组织中瘦素和FTO基因调控的关系
IF 3 3区 生物学
Amino Acids Pub Date : 2025-01-11 DOI: 10.1007/s00726-024-03441-2
Farshad Teymoori, Hossein Farhadnejad, Mostafa Norouzzadeh, Mitra Kazemi Jahromi, Niloufar Saber, Ebrahim Mokhtari, Golaleh Asghari, Emad Yuzbashian, Parvin Mirmiran, Alireza Khalaj, Maryam Zarkesh, Mehdi Hedayati, Mohammadreza Vafa
{"title":"The relationship between dietary branched-chain and aromatic amino acids with the regulation of leptin and FTO genes in adipose tissue of patients undergoing abdominal surgery","authors":"Farshad Teymoori,&nbsp;Hossein Farhadnejad,&nbsp;Mostafa Norouzzadeh,&nbsp;Mitra Kazemi Jahromi,&nbsp;Niloufar Saber,&nbsp;Ebrahim Mokhtari,&nbsp;Golaleh Asghari,&nbsp;Emad Yuzbashian,&nbsp;Parvin Mirmiran,&nbsp;Alireza Khalaj,&nbsp;Maryam Zarkesh,&nbsp;Mehdi Hedayati,&nbsp;Mohammadreza Vafa","doi":"10.1007/s00726-024-03441-2","DOIUrl":"10.1007/s00726-024-03441-2","url":null,"abstract":"<div><p>Recent studies have suggested that the interaction between diet and an individual’s genetic predisposition can determine the likelihood of obesity and various metabolic disorders. The current study aimed to examine the association of dietary branched-chain amino acids(BCAAs) and aromatic amino acids(AAAs) with the expression of the leptin and FTO genes in the visceral and subcutaneous adipose tissues of individuals undergoing surgery. This cross-sectional study was conducted on 136 Iranian adults, both men and women, aged ≥18 years. The samples were selected from patients admitted for abdominal surgeries. The dietary intake of BCAAs and AAAs was determined using a valid and reliable 168-item food frequency questionnaire. Using the quantitative PCR method, leptin and FTO mRNA expression was measured in both visceral and subcutaneous fat tissues. The mean age of the participants was 39.8 ± 12.7 years, and the mean intake of BCAAs and AAAs was 17.7 ± 0.9 and 9.3 ± 0.3% of protein per day, respectively. In overweight-obese patients(body mass index = 25–34.9 kg/m<sup>2</sup>), the intake of BCAAs(β:-0.75,95%CI:-1.47,-0.03), valine(β:-0.78,95%CI:-1.51,-0.05), and tyrosine(β:-0.81,95%CI:-1.55,-0.06) was inversely associated with FTO gene expression in subcutaneous fat tissue in adjusted model. In morbidly obese patients(body mass index ≥ 35 kg/m<sup>2</sup>), a higher intake of total BCAAs(β:1.10,95%CI:0.07–2.13), leucine(β:1.07,95%CI:0.03–2.13), and isoleucine(β:1.49,95%CI:0.46–2.52) was associated with an increase of leptin gene expression in subcutaneous fat tissue. Our findings suggest that dietary BCAA may associated with gene expression in adipose tissues, potentially influencing obesity-related metabolic pathways. Further prospective studies are warranted to validate results and elucidate the potential for dietary interventions targeting amino acids intake in obesity management.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03441-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retraction Note: Modulation of mercury-induced mitochondria-dependent apoptosis by glycine in hepatocytes 撤稿注:甘氨酸对汞诱导的肝细胞线粒体依赖性凋亡的调节
IF 3 3区 生物学
Amino Acids Pub Date : 2025-01-10 DOI: 10.1007/s00726-024-03439-w
Pabitra Bikash Pal, Sankhadeep Pal, Joydeep Das, Parames C. Sil
{"title":"Retraction Note: Modulation of mercury-induced mitochondria-dependent apoptosis by glycine in hepatocytes","authors":"Pabitra Bikash Pal,&nbsp;Sankhadeep Pal,&nbsp;Joydeep Das,&nbsp;Parames C. Sil","doi":"10.1007/s00726-024-03439-w","DOIUrl":"10.1007/s00726-024-03439-w","url":null,"abstract":"","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03439-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taurine prevents mitochondrial dysfunction and protects mitochondria from reactive oxygen species and deuterium toxicity 牛磺酸可以防止线粒体功能障碍,保护线粒体免受活性氧和氘的毒害
IF 3 3区 生物学
Amino Acids Pub Date : 2025-01-10 DOI: 10.1007/s00726-024-03440-3
Stephanie Seneff, Anthony M. Kyriakopoulos
{"title":"Taurine prevents mitochondrial dysfunction and protects mitochondria from reactive oxygen species and deuterium toxicity","authors":"Stephanie Seneff,&nbsp;Anthony M. Kyriakopoulos","doi":"10.1007/s00726-024-03440-3","DOIUrl":"10.1007/s00726-024-03440-3","url":null,"abstract":"<div><p>Taurine, although not a coding amino acid, is the most common free amino acid in the body. Taurine has multiple and complex functions in protecting mitochondria against oxidative-nitrosative stress. In this comprehensive review paper, we introduce a novel potential role for taurine in protecting from deuterium (heavy hydrogen) toxicity. This can be of crucial impact to either normal or cancer cells that have highly different mitochondrial redox status. Deuterium is an isotope of hydrogen with a neutron as well as a proton, making it about twice as heavy as hydrogen. We first explain the important role that the gut microbiome and the gut sulfomucin barrier play in deuterium management. We describe the synergistic effects of taurine in the gut to protect against the deleterious accumulation of deuterium in the mitochondria, which disrupts ATP synthesis by ATPase pumps. Moreover, taurine’s derivatives, N-chlorotaurine (NCT) and N-bromotaurine (NBrT), produced through spontaneous reaction of taurine with hypochlorite and hypobromite, have fascinating regulatory roles to protect from oxidative stress and beyond. We describe how taurine could potentially alleviate deuterium stress, primarily through metabolic collaboration among various gut microflora to produce deuterium depleted nutrients and deuterium depleted water, and in this way protect against leaky gut barrier, inflammatory bowel disease, and colon cancer.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03440-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isoaspartate formation and irreversible aggregation of collapsin response mediator protein 2: implications for the etiology of epilepsy and age-related cognitive decline 异天冬氨酸的形成和不可逆聚集的塌陷反应介质蛋白2:癫痫和年龄相关的认知能力下降的病因学意义
IF 3 3区 生物学
Amino Acids Pub Date : 2024-12-24 DOI: 10.1007/s00726-024-03435-0
Jeff X. Zhu, Dana W. Aswad
{"title":"Isoaspartate formation and irreversible aggregation of collapsin response mediator protein 2: implications for the etiology of epilepsy and age-related cognitive decline","authors":"Jeff X. Zhu,&nbsp;Dana W. Aswad","doi":"10.1007/s00726-024-03435-0","DOIUrl":"10.1007/s00726-024-03435-0","url":null,"abstract":"<div><p>Collapsin response mediator protein 2 (CRMP2) functions in the genesis and activity of neuronal connections in mammalian brain. We previously reported that a protein coincident with CRMP2 on 2D-gels undergoes marked accumulation of abnormal L-isoaspartyl sites in brain extracts of mice missing the repair enzyme, protein L-isoaspartyl methyltransferase (PIMT). To confirm and explore the significance of isoaspartyl damage in CRMP2, we expressed and purified recombinant mouse CRMP2 (rCRMP2). A polyclonal antibody made against the recombinant protein precipitated CRMP2 from brain extracts of PIMT-KO mice, but not from WT mice, suggesting that (1) the rCRMP2 antigen underwent significant isoAsp formation in the process of antibody production and (2) the isoAsp form of CRMP2 is considerably more immunogenic than the native protein. In vitro aging of rCRMP2 at pH 7.4, 37 °C for 0–28 days led to robust accumulation of isoAsp sites that were repairable by PIMT, and also induced a progressive accumulation of apparent dimers and higher-mass oligomers as judged by SDS-PAGE. A similar pattern of CRMP2 aggregation was observed in mice, with levels increasing throughout the lifespan. We conclude that CRMP2 is indeed a major target of PIMT-mediated protein repair in the brain; that isoAsp forms of CRMP2 are highly immunogenic; and that CRMP2 dysfunction makes a significant contribution to neuropathology in the PIMT-KO mouse.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03435-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive analysis of peripheral blood free amino acids in MASLD: the impact of glycine-serine-threonine metabolism MASLD外周血游离氨基酸的综合分析:对甘氨酸-丝氨酸-苏氨酸代谢的影响
IF 3 3区 生物学
Amino Acids Pub Date : 2024-12-24 DOI: 10.1007/s00726-024-03433-2
Masaaki Mino, Eiji Kakazu, Akitoshi Sano, Mio Tsuruoka, Hiroko Matsubara, Keisuke Kakisaka, Takayuki Kogure, Katsunori Sekine, Yoshihiko Aoki, Masatoshi Imamura, Michitaka Matsuda, Taiji Yamazoe, Taizo Mori, Sachiyo Yoshio, Jun Inoue, Atsushi Masamune, Tatsuya Kanto
{"title":"Comprehensive analysis of peripheral blood free amino acids in MASLD: the impact of glycine-serine-threonine metabolism","authors":"Masaaki Mino,&nbsp;Eiji Kakazu,&nbsp;Akitoshi Sano,&nbsp;Mio Tsuruoka,&nbsp;Hiroko Matsubara,&nbsp;Keisuke Kakisaka,&nbsp;Takayuki Kogure,&nbsp;Katsunori Sekine,&nbsp;Yoshihiko Aoki,&nbsp;Masatoshi Imamura,&nbsp;Michitaka Matsuda,&nbsp;Taiji Yamazoe,&nbsp;Taizo Mori,&nbsp;Sachiyo Yoshio,&nbsp;Jun Inoue,&nbsp;Atsushi Masamune,&nbsp;Tatsuya Kanto","doi":"10.1007/s00726-024-03433-2","DOIUrl":"10.1007/s00726-024-03433-2","url":null,"abstract":"<div><p>Little is known about how blood free amino acids (FAAs) change in metabolic dysfunction-associated steatotic liver disease (MASLD). This study aims to identify the imbalance of FAAs in MASLD and explore its correction as a potential therapeutic target. We analyzed plasma FAAs data from 23,036 individuals with steatosis information from a biobank in Japan, and 310 patients with MASLD were enrolled. According to diagnostic criteria for steatotic liver disease (SLD) or cardiometabolic criteria (CC), we divided the subjects into five groups: MASLD, metabolic dysfunction and alcohol-associated liver disease (MetALD), CC-SLD-, CC + SLD-, and CC-SLD + . Twenty FAAs were compared among these groups and among MASLD patients with pathological information. Among the 20 FAAs, the levels of 16 FAAs increased in CC + SLD- according to the number of matches with CC items associated with insulin resistance (IR). Steatosis enhanced most of these changes but serine (Ser) and threonine (Thr) were unaffected. Glycine (Gly), Ser and Thr were significantly decreased in patients according to steatosis grade. We investigated the association between these FAAs imbalances and pathogenesis using MASLD mouse models. In mice fed a high-fat, fructose, and cholesterol (FFC) diet, metabolomics and RNA sequencing analyses indicated that abnormality in Gly, Ser, and Thr metabolism in the liver was associated with mitochondrial dysfunction and enhanced glycolysis via pyruvate. High-Gly, Ser, and Thr diet ameliorated pathogenesis of MASLD in leptin-deficient mice. Most FAAs increase due to cardiometabolic abnormalities, particularly IR. However, interventions targeting the metabolism of Gly, Ser, and Thr have the potential to improve MASLD.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03433-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of D-amino acid metabolic enzyme deficiency on cancer development—diffuse large B-cell lymphoma onset and gene expression analyses in DASPO-knockout mice d -氨基酸代谢酶缺乏对daspo基因敲除小鼠肿瘤发展-弥漫性大b细胞淋巴瘤发病及基因表达的影响
IF 3 3区 生物学
Amino Acids Pub Date : 2024-12-24 DOI: 10.1007/s00726-024-03426-1
Yusuke Nakade, Yasunori Iwata, Kenichi Harada, Yasuharu Sato, Masashi Mita, Kenji Hamase, Ryuichi Konno, Mayo Hayashi, Taku Kobayashi, Yuta Yamamura, Tadashi Toyama, Atsushi Tajima, Takashi Wada
{"title":"Effect of D-amino acid metabolic enzyme deficiency on cancer development—diffuse large B-cell lymphoma onset and gene expression analyses in DASPO-knockout mice","authors":"Yusuke Nakade,&nbsp;Yasunori Iwata,&nbsp;Kenichi Harada,&nbsp;Yasuharu Sato,&nbsp;Masashi Mita,&nbsp;Kenji Hamase,&nbsp;Ryuichi Konno,&nbsp;Mayo Hayashi,&nbsp;Taku Kobayashi,&nbsp;Yuta Yamamura,&nbsp;Tadashi Toyama,&nbsp;Atsushi Tajima,&nbsp;Takashi Wada","doi":"10.1007/s00726-024-03426-1","DOIUrl":"10.1007/s00726-024-03426-1","url":null,"abstract":"<div><p>The relationship between D-AA metabolic enzymes and cancer development remains unclear. We aimed to investigate this relationship using mice deficient in D-AA-related metabolic enzymes. We examined mice lacking these enzymes for approximately 900 days and the effects of altered D-AA metabolism on cancer development based on lifespan, pathological findings, and gene expression. The lifespan of female <i>DASPO</i> -knockout (<i>DASPO</i><sup><i>−/−</i></sup>) mice was shorter than that of the other group mice; furthermore, these mice showed tumor-like masses in the liver, spleen, and small intestine. A pathological diagnosis of diffuse large B-cell lymphoma (DLBCL) was made. RNA sequencing of the liver samples showed specific alterations in the expression of 71 genes in <i>DASPO</i><sup><i>−/−</i></sup> mice compared with that in wild-type B6 mice; <i>RGS 1</i>, <i>MTSS1</i>, and <i>SMARCD 1</i> were identified as DLBCL-related genes. Patients with DLBCL exhibiting low <i>DASPO</i> expression demonstrated a shorter survival period than those showing high expression. However, the role of <i>DASPO</i> in DLBCL development is unclear. Therefore, future research should focus on B cells. <i>DASPO</i> may serve as novel biomarkers and therapeutic targets in cancer.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03426-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dipeptides in CSF and plasma: diagnostic and therapeutic potential in neurological diseases 脑脊液和血浆中的二肽:神经系统疾病的诊断和治疗潜力
IF 3 3区 生物学
Amino Acids Pub Date : 2024-12-13 DOI: 10.1007/s00726-024-03434-1
Katharina Küper, Gernot Poschet, Julia Rossmann, Sven F. Garbade, Alexander Spiegelhalter, Dan Wen, Georg F. Hoffmann, Claus P. Schmitt, Thomas Opladen, Verena Peters
{"title":"Dipeptides in CSF and plasma: diagnostic and therapeutic potential in neurological diseases","authors":"Katharina Küper,&nbsp;Gernot Poschet,&nbsp;Julia Rossmann,&nbsp;Sven F. Garbade,&nbsp;Alexander Spiegelhalter,&nbsp;Dan Wen,&nbsp;Georg F. Hoffmann,&nbsp;Claus P. Schmitt,&nbsp;Thomas Opladen,&nbsp;Verena Peters","doi":"10.1007/s00726-024-03434-1","DOIUrl":"10.1007/s00726-024-03434-1","url":null,"abstract":"<div><p>Dipeptides (DPs), composed of two amino acids (AAs), hold significant therapeutic potential but remain underexplored. Given the crucial role of AAs in central nervous system (CNS) function, this study investigated the presence of DPs in cerebrospinal fluid (CSF) and their correlation with corresponding AAs, potentially indicating their role as AA donors. Plasma and CSF samples were collected from 43 children with neurological or metabolic conditions of unknown origin, including 23 with epilepsy. A panel of 33 DPs was quantified using UPLC-MS/MS. Out of 33 DPs, 18 were detectable in CSF and 20 in plasma, displaying high inter-individual variance. Gly-Asp, Gly-Pro, and Ala-Glu were consistently found in all CSF samples, while only Gly-Asp was universally detectable in plasma. Anserine and carnosine were prominent in CSF and plasma, respectively, with no other histidine-containing DPs observed. Generally, DP concentrations were higher in plasma than in CSF; however, anserine and Gly-Pro had similar concentrations in both fluids. Significant correlations were observed between specific DPs and their corresponding AAs in CSF (Gly-Glu, Gly-Pro and Ser-Gln) and plasma (Glu-Glu and Glu-Ser). Notably, patients with epilepsy had elevated medium anserine concentrations in CSF. This study is the first to demonstrate the presence of numerous DPs in CSF and plasma. Further research is needed to determine if DP patterns can support the diagnosis of neurological diseases and whether DP administration can modulate amino acid availability in the brain, potentially offering new therapeutic options, such as for defects in the amino acid transporter.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-024-03434-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Free amino acids accelerate the time-dependent inactivation of rat liver nucleotide pyrophosphatase/phosphodiesterase Enpp3 elicited by EDTA 游离氨基酸加速了EDTA诱导的大鼠肝脏核苷酸焦磷酸酶/磷酸二酯酶Enpp3的时间依赖性失活。
IF 3 3区 生物学
Amino Acids Pub Date : 2024-12-06 DOI: 10.1007/s00726-024-03431-4
Ana Romero, Guadalupe Cumplido-Laso, Ascensión Fernández, Javier Moreno, José Canales, Rui Ferreira, Juan López-Gómez, João Meireles Ribeiro, María Jesús Costas, José Carlos Cameselle
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