Metabolomic analysis reveals key changes in amino acid metabolism in colorectal cancer patients

IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Asmaa Ramzy, Taghreed Khaled Abdelmoneim, Menna Arafat, Maha Mokhtar, Ashraf Bakkar, Amany Mokhtar, Wagida Anwar, Sameh Magdeldin, Shymaa Enany
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Abstract

The number of colorectal cancer (CRC) patients is steadily growing worldwide, particularly in developing nations. Nonetheless, recent advances in early detection studies and therapy alternatives have reduced CRC mortality in affluent countries, despite rising incidence. Gut microbiota and their metabolites may contribute to tumor growth and reduced therapeutic efficacy. This preliminary study sought to uncover metabolic fingerprints in colorectal cancer patients. It also emphasizes the correlation between the gut microbiome, microbial metabolism, and altered metabolites in CRC. In this study, stool samples from 20 CRC patients and matched healthy controls were enrolled. Untargeted metabolomics approach based on an ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-MS/MS) were applied. Statistical approaches, pathway enrichment analysis, and network analysis were employed to unleash CRC perturbed metabolic pathways and putative biomarkers. The study identified a distinct manually curated metabolite profile that is substantially linked to CRC. The steroidogenesis, aspartate, tryptophan (Trp), and urea cycle were the most significant pathways that concurrently contributed to CRC.Prominently, among other pathways, Trp metabolism was identified as a critical pathway, indicating a possible connection between the development of CRC and gut microbiota. In a nutshell the notable resulted metabolites reveal auspicious biomarkers for the initial diagnosis as well as surveilling of CRC progression. This preliminary study highlights the potential involvement that gut bacteria may contribute in CRC patients. Further investigation into the composition of the gut microbiome associated with this metabolic profile may lead to the identification of novel biomarkers for early detection and possible targets for treatment.

代谢组学分析揭示了结直肠癌患者氨基酸代谢的关键变化
结直肠癌(CRC)患者的数量在全球范围内稳步增长,特别是在发展中国家。尽管如此,尽管发病率上升,但在富裕国家,早期发现研究和治疗方案的最新进展降低了结直肠癌的死亡率。肠道菌群及其代谢产物可能促进肿瘤生长,降低治疗效果。这项初步研究旨在揭示结直肠癌患者的代谢指纹。它还强调了CRC中肠道微生物组、微生物代谢和代谢物改变之间的相关性。在这项研究中,来自20名CRC患者和匹配的健康对照者的粪便样本被纳入研究。采用基于超高效液相色谱-高分辨率质谱(UHPLC-MS/MS)的非靶向代谢组学方法。采用统计方法、途径富集分析和网络分析来释放CRC紊乱的代谢途径和假定的生物标志物。该研究确定了一种独特的人工管理的代谢物谱,它与结直肠癌有很大的联系。甾体生成、天冬氨酸、色氨酸和尿素循环是并发结直肠癌的最重要途径。值得注意的是,在其他途径中,Trp代谢被认为是一个关键途径,表明CRC的发展与肠道微生物群之间可能存在联系。简而言之,显著的代谢物结果揭示了初步诊断和监测结直肠癌进展的吉祥生物标志物。这项初步研究强调了肠道细菌可能参与结直肠癌患者的潜在作用。进一步研究与这种代谢谱相关的肠道微生物组的组成可能会导致鉴定出用于早期检测的新型生物标志物和可能的治疗靶点。
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来源期刊
Amino Acids
Amino Acids 生物-生化与分子生物学
CiteScore
6.40
自引率
5.70%
发文量
99
审稿时长
2.2 months
期刊介绍: Amino Acids publishes contributions from all fields of amino acid and protein research: analysis, separation, synthesis, biosynthesis, cross linking amino acids, racemization/enantiomers, modification of amino acids as phosphorylation, methylation, acetylation, glycosylation and nonenzymatic glycosylation, new roles for amino acids in physiology and pathophysiology, biology, amino acid analogues and derivatives, polyamines, radiated amino acids, peptides, stable isotopes and isotopes of amino acids. Applications in medicine, food chemistry, nutrition, gastroenterology, nephrology, neurochemistry, pharmacology, excitatory amino acids are just some of the topics covered. Fields of interest include: Biochemistry, food chemistry, nutrition, neurology, psychiatry, pharmacology, nephrology, gastroenterology, microbiology
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