Amino Acids最新文献

{"title":"Enzyme inhibitors for drug discovery","authors":"Patrick Meffre, Zohra Benfodda, Sébastien Albrecht","doi":"10.1007/s00726-023-03357-3","DOIUrl":"10.1007/s00726-023-03357-3","url":null,"abstract":"","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1707 - 1708"},"PeriodicalIF":3.5,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid","authors":"Shakti K. Maurya,&nbsp;Abhishek Tripathi,&nbsp;Selvakumar Karuthapandi,&nbsp;Harkesh B. Singh","doi":"10.1007/s00726-023-03348-4","DOIUrl":"10.1007/s00726-023-03348-4","url":null,"abstract":"<div><p>The conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (<b>1</b>) and SeC-Lipoic acid (<b>2</b>) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (¹H, ¹³C, ⁷⁷Se) and 2D (¹H-¹H COSY and ¹H-¹³C TOCSY) NMR spectroscopy, ESI–MS spectrometry, and RP-HPLC. The GPx-like enzyme mimicking activity of the SeC-bioconjugates <b>1</b> and <b>2</b> has been investigated through the coupled reductase assay method for the catalytic reductions of hydrogen peroxide into water_. A significant enhancement in GPx-like enzymatic activity was observed for both novel bioconjugates SeC-Biotin (<b>1</b>) and SeC-Lipoic acid (<b>2</b>) as compared to diphenyl diselenide (Ph₂Se₂), L-selenocystine (SeC), biotin, lipoic acid, and ebselen.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1981 - 1989"},"PeriodicalIF":3.5,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136395917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino acid-stimulated insulin secretion: a path forward in type 2 diabetes","authors":"Jelena Kolic,&nbsp;WenQing Grace Sun,&nbsp;James D. Johnson,&nbsp;Nicola Guess","doi":"10.1007/s00726-023-03352-8","DOIUrl":"10.1007/s00726-023-03352-8","url":null,"abstract":"<div><p>Qualitative and quantitatively appropriate insulin secretion is essential for optimal control of blood glucose. Beta-cells of the pancreas produce and secrete insulin in response to glucose and non-glucose stimuli including amino acids. In this manuscript, we review the literature on amino acid-stimulated insulin secretion in oral and intravenous in vivo studies, in addition to the in vitro literature, and describe areas of consensus and gaps in understanding. We find promising evidence that the synergism of amino acid-stimulated insulin secretion could be exploited to develop novel therapeutics, but that a systematic approach to investigating these lines of evidence is lacking. We highlight evidence that supports the relative preservation of amino acid-stimulated insulin secretion compared to glucose-stimulated insulin secretion in type 2 diabetes, and make the case for the therapeutic potential of amino acids. Finally, we make recommendations for research and describe the potential clinical utility of nutrient-based treatments for type 2 diabetes including remission services.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1857 - 1866"},"PeriodicalIF":3.5,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel antibacterial approach of Cecropin-B peptide loaded on chitosan nanoparticles against MDR Klebsiella pneumoniae isolates","authors":"Hend Okasha,&nbsp;Heba Dahroug,&nbsp;Abdullah E. Gouda,&nbsp;Mohamed Abbas Shemis","doi":"10.1007/s00726-023-03356-4","DOIUrl":"10.1007/s00726-023-03356-4","url":null,"abstract":"<div><p>Egypt has witnessed the emergence of multidrug-resistant (MDR) <i>Klebsiella pneumoniae</i>, which has posed a serious healthcare challenge. The proper treatment choice for MDR-KP infections is not well determined which renders the problem more complicated, thus making the control of such infections a serious challenge for healthcare professionals. This study aims to encapsulate the cationic antimicrobial peptide; Cecropin-B (Cec-B), to increase its lifetime, drug targeting, and efficacy and study the antimicrobial effect of free and encapsulated recombinant rCec-B peptide on multidrug-resistant <i>K. pneumoniae</i> (MDR-KP) isolates. Fifty isolates were collected from different clinical departments at Theodore Bilharz Research Institute. Minimal inhibitory concentrations (MICs) of rCec-B against MDR-KP isolates were determined by the broth microdilution test. In addition, encapsulation of rCec-B peptide into chitosan nanoparticles and studying its bactericidal effect against MDR-KP isolates were also performed. The relative expression of efflux pump and porin coding genes (<i>ArcrB</i>, <i>TolC</i>, <i>mtdK</i>, and <i>Ompk35</i>) was detected by quantitative PCR in treated MDR-KP bacterial isolates compared to untreated isolates. Out of 60 clinical MDR isolates, 50 were MDR-KP. 60% of the isolates were XDR while 40% were MDR. rCec-B were bactericidal on 21 isolates, then these isolates were subjected to treatment using free nanocapsule in addition to the encapsulated peptide. Free capsules showed a mild cytotoxic effect on MDR-KP at the highest concentration. MIC of encapsulated rCec-B was higher than the free peptide. The expression level of genes encoding efflux and porin (<i>ArcrB</i>, <i>TolC</i>, <i>mtdK</i>, and <i>Ompk35</i>) was downregulated after treatment with encapsulated rCec-B. These findings indicate that encapsulated rCec-B is a promising candidate with potent antibacterial activities against drug-resistant <i>K. pneumoniae.</i></p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1965 - 1980"},"PeriodicalIF":3.5,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10724327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety assessment of L-Arg oral intake in healthy subjects: a systematic review of randomized control trials","authors":"Yui Kuramochi,&nbsp;Mai Murata,&nbsp;Akihide Sumino,&nbsp;Hideko Sone,&nbsp;Kohsuke Hayamizu","doi":"10.1007/s00726-023-03354-6","DOIUrl":"10.1007/s00726-023-03354-6","url":null,"abstract":"<div><p>L-Arg is a nonessential amino acid but has many physiological roles. Accordingly, L-Arg has been used in various fields, but there is only limited information available about its safety upon overdose. Generally, the no-observed adverse effect level (NOAEL) is used when setting the upper amount for chemical substances. Recently, systematic reviews have been used to assess the safety as well as the effectiveness and usefulness of them. Therefore, we conducted an assessment of the safety of the oral intake of L-Arg in healthy subjects using gastrointestinal symptoms as an index. We limited the study design to only double-blind randomized controlled trials and searched PubMed, Cochrane Library, EBSCOhost, and Ichushi-Web from inception until May 2021. Assessment of the quality of studies was conducted using the Cochrane Collaboration tool and Jadad score, and the random effects model was used for data analysis. Ultimately, 34 studies were selected for inclusion in this work. The dosage of L-Arg used in the studies ranged from 2000 to 30,000 mg/day (or/one-time dose), and the treatment duration was 1–84 days. The increased risk of gastrointestinal symptoms associated with L-Arg intake from 23 studies (647 participants in total) in which such symptoms were reported was 0.01 (95% confidence interval: – 0.02–0.04), which was not significant difference. NOAEL was estimated as 7531 mg/ one-time dose using a weighted change-point regression model (UMIN000046133).</p><p><i>Registration and protocol</i>: Umin.ac.jp as UMIN000046133.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1949 - 1964"},"PeriodicalIF":3.5,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10724322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72013164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of milk-derived bioactive peptides as antidiabetic, antihypertensive, and xanthine oxidase inhibitors: a comprehensive bibliometric analysis and updated review","authors":"Emyr Hiago Bellaver,&nbsp;Aniela Pinto Kempka","doi":"10.1007/s00726-023-03351-9","DOIUrl":"10.1007/s00726-023-03351-9","url":null,"abstract":"<div><p>Bioactive peptides consist of small protein fragments, which are inactive in their original conformation, and they become active when released from these through enzymatic hydrolysis or fermentation processes. The bioactivity of such peptides has been extensively reported in the literature as contributors to organic homeostasis processes, as well as in immunomodulation, organism defense against oxidative processes, among others. In this study, reports of the activity of BPs isolated from milk with the potential glycemic control, antihypertensive activity, and inhibitors of uric acid formation were compiled. A systematic literature review and bibliometric analysis were conducted, using the PICO strategy for the research. The temporal analysis of publications revealed a growing interest in the investigation of bioactive peptides with potential antidiabetic, antihypertensive, and xanthine oxidase inhibitory activities, using dairy sources as products for their extraction. The literature analysis also revealed an increase in research involving non-bovine dairy products for bioactive peptide extraction. The collaboration network among authors exhibited weaknesses in scientific cooperation. Regarding the analysis of keywords, the usage of terms such as “bioactive peptides”, “antioxidant”, “antihypertensive”, and “diabetes” was evident, constituting the main research clusters. Peptides with low molecular weight, typically &lt; 10 kDa, of hydrophobic nature with aliphatic and aromatic chains, have significant implications in molecular interactions for the required activities. Although there is a growing interest in the industry regarding the utilization of bioactive peptides as potential drugs, there is a need to address gaps related to elucidating their interactions with cellular targets and their use in human therapy.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1829 - 1855"},"PeriodicalIF":3.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating molecular interactions between human transferrin and resveratrol through a unified experimental and computational approach: Role of natural compounds in Alzheimer’s disease therapeutics","authors":"Mohd Shahnawaz Khan,&nbsp;Mohammad Furkan,&nbsp;Moyad Shahwan,&nbsp;Dharmendra Kumar Yadav,&nbsp;Saleha Anwar,&nbsp;Rizwan Hasan Khan,&nbsp;Anas Shamsi","doi":"10.1007/s00726-023-03355-5","DOIUrl":"10.1007/s00726-023-03355-5","url":null,"abstract":"<div><p>Disruptions to iron metabolism and iron homeostasis have emerged as significant contributors to the development and progression of Alzheimer's disease (AD). Human transferrin plays a key part in maintaining iron equilibrium throughout the body, highlighting its importance in AD. Many plant-derived compounds and dietary constituents show promise for preventing AD. Polyphenols that are abundant in fruits, vegetables, teas, coffee, and herbs possess neuroprotective attributes. Resveratrol is a natural polyphenol present in various plant sources like grapes, berries, peanuts, and red wine that has garnered research interest due to its wide range of biological activities. Notably, resveratrol exhibits neuroprotective effects that may help prevent or treat AD through multiple mechanisms. In the present study, we employed a combination of molecular docking and all-atom molecular dynamic simulations (MD) along with experimental approaches to unravel the intricate interactions between transferrin and resveratrol deciphering the binding mechanism. Through molecular docking analysis, it was determined that resveratrol occupies the iron binding pocket of transferrin. Furthermore, MD simulations provided a more profound insight into the stability and conformational dynamics of the complex suggesting that the binding of resveratrol introduced localized flexibility, while maintaining overall stability. The spectroscopic observations yielded clear evidence of substantial binding between resveratrol and transferrin, confirming the computational findings. The identified binding mechanism and conformational stability hold potential for advancing the development of innovative therapeutic approaches targeting AD through resveratrol, particularly concerning iron homeostasis. These insights serve as a platform for considering the natural compounds in the realm of AD therapeutics.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1923 - 1935"},"PeriodicalIF":3.5,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered kynurenine pathway metabolism and association with disease activity in patients with systemic lupus","authors":"Duygu Eryavuz Onmaz,&nbsp;Dilek Tezcan,&nbsp;Sema Yilmaz,&nbsp;Mustafa Onmaz,&nbsp;Ali Unlu","doi":"10.1007/s00726-023-03353-7","DOIUrl":"10.1007/s00726-023-03353-7","url":null,"abstract":"<div><p>Systemic lupus erythematosus (SLE) is an autoimmune disease accompanied by increased release of proinflammatory cytokines that are known to activate the indoleamine 2,3-dioxygenase (IDO-1) enzyme, which catalyzes the rate-limiting step of the kynurenine pathway (KP). This study aimed to measure KP metabolite levels in patients with SLE and investigate the relationship between disease activity, clinical findings, and KP. The study included 100 patients with SLE and 100 healthy controls. Serum tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), 3-hydroxyanthranilic acid (3HAA), 3-hydroxykynurenine (3HK), quinolinic acid (QA) concentrations were measured with tandem mass spectrometry. Serum KYN, KYNA, 3HAA, 3HK, and QA levels of the patients with SLE were significantly higher than the control group. Serum QA levels were elevated in patients with neurological involvement (four patients with peripheral neuropathy and two patients with mononeuropathy), serum KYN levels and KYN/TRP ratio increased in patients with joint involvement, and serum KYN, 3HK, and 3HAA levels and the KYN/TRP ratio were increased in patients with renal involvement. Moreover, KYN and KYN/TRP ratios were positively correlated with the disease activity score. These findings indicated that imbalances in KP metabolites may be associated with the pathogenesis, activation, and clinical manifestations of SLE.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1937 - 1947"},"PeriodicalIF":3.5,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring details about structure requirements based on antioxidant tripeptide derived from β-Lactoglobulin by in silico approaches","authors":"Fangfang Wang,&nbsp;Menghao Wen,&nbsp;Bo Zhou","doi":"10.1007/s00726-023-03350-w","DOIUrl":"10.1007/s00726-023-03350-w","url":null,"abstract":"<div><p><i>β</i>-Lactoglobulin is one of the proteins in milk possessing antioxidant activity. The peptides derived from <i>β</i>-Lactoglobulin exhibit higher antioxidant activities than the most commonly used antioxidant. Furthermore, the detailed structure–activity relationship of these antioxidant peptides has not been elucidated. Therefore, in the present work, two-dimensional quantitative structure–activity relationship (2D-QSAR) and three-dimensional quantitative structure–activity relationship (3D-QSAR) models were constructed to investigate the structural factors affecting activities and gave information for the rational design of novel antioxidant peptides. After calculation and screening of molecular descriptors, linear and nonlinear models were developed by multiple linear regression (MLR), partial least squares regression (PLSR) and support vector machines (SVM) approaches. The statistical parameters are as follows: R² = 0.643, Q² = 0.553/MLR, R² = 0.612, Q² = 0.5278/PLSR, R² = 0.7085, Q² = 0.6887/SVM, indicating that the SVM model is superior to the MLR and PLSR models. In addition, in the 3D-QSAR models, the Dragon-CoMFA (R²_cv = 0.537, R²_pred = 0.5201) and Dragon-CoMSIA (R²_cv = 0.665, R²_pred = 0.6489) methods were conducted to predict the antioxidant activities. Comparison of statistical parameters illustrates that the suitability of Dragon-CoMSIA is superior to the Dragon-CoMFA model. The results show the robustness and excellent prediction of the proposed models, and would be applied for modifying and designing novel and potent antioxidant peptides.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1909 - 1922"},"PeriodicalIF":3.5,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71419915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of lipopeptide extracted from Bacillus licheniformis on the expression of bap and luxI genes in multi-drug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa","authors":"Farangis Ghanipour,&nbsp;Razieh Nazari,&nbsp;Seyed Soheil Aghaei,&nbsp;Parvaneh Jafari","doi":"10.1007/s00726-023-03346-6","DOIUrl":"10.1007/s00726-023-03346-6","url":null,"abstract":"<div><p>Recently, opportunistic pathogens like <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> have caused concern due to their ability to cause antibiotic resistance in weakened immune systems. As a result, researchers are always seeking efficient antimicrobial agents to tackle this issue. The hypothesis of the recent study was that probiotic products derived from bacteria would be effective in reducing drug resistance in other bacteria. This research aimed to investigate the antimicrobial properties of probiotic products from various bacterial strains, including <i>Lactobacillus rhamnosus</i>, <i>Pediococcus acidilactisi</i>, <i>Bacillus coagulans</i>, <i>Bacillus subtilis</i>, and <i>Bacillus licheniformis</i>. These were tested against multi-drug-resistant (MDR) standard strains <i>A. baumannii</i> and <i>P. aeruginosa</i>. <i>B. licheniformis</i> was found to be the most effective probiotic strain, possessing the <i>LanA</i> and <i>LanM</i> lantibiotic genes. The lipopeptide nature of the probiotic product was confirmed through high-performance liquid chromatography (HPLC) and Fourier-transform infrared spectroscopy (FTIR) techniques. The anti-biofilm and antimicrobial properties of this probiotic were measured using an SEM electron microscope and minimum inhibitory concentration (MIC) test. Real-time PCR (qPCR) was used to compare the expression of <i>bap</i> and <i>luxI</i> genes, which are considered virulence factors of drug-resistant bacteria, before and after treatment with antimicrobial agents. The MIC results showed that the probiotic product prevented the growth of bacteria at lower concentrations compared to antibiotics. In addition, the ΔΔCqs indicated that gene expression was significantly down-regulated following treatment with the obtained probiotic product. It was found that <i>B. licheniformis</i> probiotic products could reduce drug resistance in other bacteria, making it a potential solution to antibiotic resistance.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"55 12","pages":"1891 - 1907"},"PeriodicalIF":3.5,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71419914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}