Raiany T Nogueira, Suzete R Gomes, Monica A Fernandez, Kevin P Barbosa, Arielly Kelly P DE Sousa, Carolina R Marchi, Silvana C Thiengo
{"title":"Phylogeny, morphology, and haplotypic distribution of Biomphalaria straminea populations from the five geographic regions of Brazil.","authors":"Raiany T Nogueira, Suzete R Gomes, Monica A Fernandez, Kevin P Barbosa, Arielly Kelly P DE Sousa, Carolina R Marchi, Silvana C Thiengo","doi":"10.1590/0001-3765202420230770","DOIUrl":"10.1590/0001-3765202420230770","url":null,"abstract":"<p><p>Biomphalaria straminea is one of the three snails that act as the intermediate hosts of Schistosoma mansoni and is responsible for maintaining high rates of schistosomiasis in some areas of northeastern Brazil. The principal morphological characteristic of B. straminea is the marked vaginal wrinkling, although it is also present in congeneric species, a group known as the B. straminea complex. Based on the morphological and molecular analyses, we investigated the intraspecific variation of B. straminea. The specimens were obtained from 10 sites in Brazil, and the shells were analyzed, as well as 16 morphological structures of the reproductive system. The COI and ITS2 sequences were used for phylogenetic analysis, genetic divergence, and haplotype network (COI). We observed a large intraspecific variation in the morphological structures examined. The genetic divergence also demonstrated significant intraspecific variability in B. straminea: 0-3% in ITS2 sequences, and 0-6% in COI sequences. Analysis of the distribution of COI haplotypes recovered 16 haplotypes and haplotype diversity of 0.9088. These results indicate phenotypic variability that is not constrained to a locality or strictly controlled genetically by B. straminea, which may have driven a misidentified of these species.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20230770"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Orlando A Silva, Josenir T Câmara, Kaliane D Leal, Fernando S Carvalho-Filho
{"title":"Flesh flies (Diptera: Sarcophagidae) from transitional Caatinga-Cerrado areas in the state of Piauí, Northeast Brazil.","authors":"José Orlando A Silva, Josenir T Câmara, Kaliane D Leal, Fernando S Carvalho-Filho","doi":"10.1590/0001-3765202420240388","DOIUrl":"https://doi.org/10.1590/0001-3765202420240388","url":null,"abstract":"<p><p>The Sarcophagidae family (Diptera) encompasses a group of flies of significant ecological importance. Additionally, numerous species within this family hold substantial value in the realms of medicine, veterinary and forensic science. The objective of this study was to conduct a comprehensive survey of Sarcophagidae species within transitional zones between the Caatinga and Cerrado biomes in the Northeastern region of Brazil. To achieve this, a total of 15 field expeditions were conducted across three distinct phytophysiognomies-riparian, vereda, and a segment of shrubby Caatinga-from 2019 to 2021. A total of 16 genera and 40 species were collected. Among them, a new species for science (Titanogrypa (Airypel) sp. nov.), Emblemasoma emblemasoma Dodge is newly recorded from South America/Brazil and the Caatinga biome, and three species constitute new records for the Northeast region (Dexosarcophaga patiuorum Santos, Pape & Mello-Patiu, Lepidodexia (Notochaeta) fumipennis (Lopes), and Oxysarcodexia meridionalis (Engel)). The most abundant species were Oxysarcodexia thornax (Walker), Oxysarcodexia avuncula (Lopes), and Argoravinia (Argoravinia) catiae Carvalho Filho & Esposito, collectively constituting 56.11% of the entire collected sample. The distribution of species across different vegetations was noted, with 19 species exclusive to riparian vegetation, three to vereda vegetation, and seven species unique to the Caatinga.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240388"},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mara Urdapilleta, Daniela Lamattina, Eliana Florencia Burgos, Oscar Daniel Salomón, Marcela Lareschi
{"title":"Ecological characterization of fleas on small mammals in natural and disturbed landscapes in the Atlantic Forest ecoregion, Argentina.","authors":"Mara Urdapilleta, Daniela Lamattina, Eliana Florencia Burgos, Oscar Daniel Salomón, Marcela Lareschi","doi":"10.1590/0001-3765202420240352","DOIUrl":"https://doi.org/10.1590/0001-3765202420240352","url":null,"abstract":"<p><p>To understand the ecoepidemiology of fleas and their interactions with domestic and wild animals, it is necessary to decode the cycles of flea abundance in relation to host species and environmental factors. The province of Misiones, known for its biodiversity and land use changes, provides an excellent study location. We assessed the diversity, prevalence, abundance, and parasitic intensity of fleas collected from 1153 rodents and opossums in various environments in the Upper Paraná Atlantic Forest of Argentina, considering phytogeography and land use. Our findings were consistent with previous studies conducted in humid regions of Argentina and the Atlantic Forest in Brazil. Hosts with high population density exhibited greater flea prevalence and abundance, and widely distributed hosts showed higher flea species richness and diversity. Significant similarities in flea similarity indices among phytogeographic areas, especially in forest. Environments with an anthropogenic gradient exhibited marked dissimilarities, with higher species richness and diversity in less disturbed sites. These parameters may be influenced by the presence of polyxenous Ctenocephalides, Polygenis, and Adoratopsylla fleas co-infesting opossum in urban areas. These findings provide valuable insights into the ecoepidemiology of fleas, their relationships with host species, and the impact of environmental factors in a strategic Atlantic forest ecoregion.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240352"},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elisa P Pellizzer, Patricia Giovanella, Adriano U DE Faria, Lara D Sette
{"title":"Marine-derived fungus Paramarasmius palmivorus CBMAI 1062 applied to sulphur indigo blue decolorization, degradation and detoxification.","authors":"Elisa P Pellizzer, Patricia Giovanella, Adriano U DE Faria, Lara D Sette","doi":"10.1590/0001-3765202420230315","DOIUrl":"https://doi.org/10.1590/0001-3765202420230315","url":null,"abstract":"<p><p>The use of marine microorganisms in the treatment of dyes and textile effluents is promising in view of their tolerance to salinity, a characteristic found in this kind of effluent. In this study, different culture conditions were applied to evaluate the decolorization, degradation, and detoxification of Sulphur Indigo Blue (SIB) by the marine-derived basidiomycete Paramarasmius palmivorus CBMAI 1062. Low salt concentration (SLS) and high salt concentration (SMASHS) media were used. P. palmivorus decolorized 100 % and 91.38 % of SIB after 120 h of growth in the SLS medium and after 168 h of growth in the SMASHS medium, respectively. Laccase activity was detected only in the SLS bioassay. UV-Vis, FT-IR, and GC-MS analyses indicated the occurrence of dye biosorption and biotransformation. In the SLS medium metabolites associated with SIB biotransformation (e.g. aldehyde, alkanes, and phenols) were detected. The toxicity measured by Cucumis sativus decreased from 45.41 % to 24.11 % in the SLS bioassay, while in SMASHS medium there was no change in toxicity. The efficiency for decolorization and detoxification of SIB indicates that microorganisms from the marine environment can be a source for biotechnological application in bioremediation processes carried out under saline conditions, adding value to blue biotechnology.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20230315"},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Paula DA S Alves, Roberta Jenniffer M Teixeira, Raissa M DA Silva, Renata DE A Canevari, Luciana B Sant'anna
{"title":"Amniotic membrane modulates MMP9 and MMP12 gene and protein expression in experimental model of the hepatic fibrosis.","authors":"Ana Paula DA S Alves, Roberta Jenniffer M Teixeira, Raissa M DA Silva, Renata DE A Canevari, Luciana B Sant'anna","doi":"10.1590/0001-3765202420231086","DOIUrl":"10.1590/0001-3765202420231086","url":null,"abstract":"<p><p>Hepatic fibrosis is characterized by excessive deposition of collagen in the hepatic parenchyma, which disturbs the normal architecture and function. We have shown that human amniotic membrane (AM) can be used as a patch on the whole liver surface, resulting in an extremely significant reduction in collagen deposition. The aim of this study was to investigate the effects of AM on the matrix metalloproteinase 9 (MMP9) and matrix metalloproteinase 12 (MMP12) genes and proteins expression by real time quantitative PCR and immunohistochemistry, respectively, as well as image analysis on biliary fibrosis induced in rats by the bile duct ligation (BDL).Two weeks after the BDL, an AM fragment was applied onto the liver, and four weeks later, the liver samples were collected. MMP9 and MMP12 genes were significantly over expressed in group treated with AM. The immunoexpression of MMP9 and MMP12 was observed in all groups. However, the quantitative image analysis demonstrated an increase of the area occupied only by MMP12 in the livers of AM-treated rats with respect to BDL rats. These findings suggest that the AM exerts its beneficial effects on biliary fibrosis by increasing the MMP12, which in turn reduces the excessive collagen deposition on liver tissue.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20231086"},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcos Aurélio A Pereira, Adilson Sartoratto, Cristina Maira T S Miguel, Cynthia Murakami, Fabiana L Silva, Inês Cordeiro, Kamilla N Machado, Maria Cláudia M Young, Telma Maria Kaneko, Patrícia S Lopes, Paulo Roberto H Moreno
{"title":"Essential oil vapor phase approach for antimicrobial evaluation using Hesperozygis myrtoides (A. St.-Hill. ex Benth.) Epling (Lamiaceae) as a model.","authors":"Marcos Aurélio A Pereira, Adilson Sartoratto, Cristina Maira T S Miguel, Cynthia Murakami, Fabiana L Silva, Inês Cordeiro, Kamilla N Machado, Maria Cláudia M Young, Telma Maria Kaneko, Patrícia S Lopes, Paulo Roberto H Moreno","doi":"10.1590/0001-3765202420231370","DOIUrl":"https://doi.org/10.1590/0001-3765202420231370","url":null,"abstract":"<p><p>The demand for less hazardous and environmentally friendly surface disinfectants is growing and essential oils may be efficient alternatives. Vapor phase assay is a promising technique which explore the volatility of essential oils for determining the antimicrobial activity. In this study, we modified the conditions of the inverted Petri dish technique to evaluate the minimum inhibitory concentrations (MIC). As a proof of concept, the antimicrobial activity of Hesperozygis myrtoides essential oil (HM) was studied for the first time in the vapor phase. According to the established protocol the oil volatilization dynamics was used to determine the major compounds in the vapor phase. After 270 min, the relative percentage was like that observed in the crude oil analysis. The HM was also evaluated for safety (IC50=921.2 µg ml-1), being considered safe in case of acute oral exposure. The oil vapors completely inhibited the growth of Staphylococcus aureus (MIC=0.39 mg l-1), Candida albicans (MIC=0.83 mg l-1), Aspergillus brasiliensis (MIC=378 mg l-1) and Bacillus subtilis (MIC=704.8 mg l-1). This protocol offers an economical and efficient alternative for antimicrobial activity and safety determination of essential oils, can be used for the evaluation of several applications of essential oils, as disinfectant agent in inhabited environments.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20231370"},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The intermodal transport tactical planning: A literature review on the use of transportation modes for cargo delivery.","authors":"Jonathan Vieira, Alvaro Neuenfeldt Júnior, Vanessa Alves, Marina Bouzon, Matheus Francescatto","doi":"10.1590/0001-3765202420240081","DOIUrl":"10.1590/0001-3765202420240081","url":null,"abstract":"<p><p>Effective freight transportation planning through different modes drives countries economic growth by ensuring the delivery of goods safely and sustainably. This article provides a systematic literature review focused on intermodal transport tactical planning, addressing logistical technical factors for efficient transportation modes management in cargo delivery. The methodology is based on the research questions definition, search process, and article selection criteria, exploring the network structures evolution to verify technical factors influencing network structures, including mode combinations, geographical distribution, and mathematical models' decision variables and constraints. According to the research question proposed, the most relevant technical factors for intermodal planning network structures are flow cargo, transport modal activation, flow conservation, delivery time, and vehicle management, generally found in countries with extensive road infrastructure and a high modal integration capacity, as China, Europe, and USA. Considering the decision variables and constraints, flow conservation is a common element observed in intermodal transport mathematical models. Thus, a balance between transportation costs and transportation modes utilization was verified, and insights into the relation between technical logistics factors application and network structures for cargo delivery efficiency were revealed. Finally, the systematic literature review contributes to understanding the main intermodal transportation planning evolving landscape, offering valuable insights for future research and practical implementations.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240081"},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Victor DE S Pereira, Diego Márlon Ferro, Janaína Carla B Machado, Magda Rhayanny A Ferreira, Luiz Alberto L Soares, Luiz Stragevitch, Leandro Danielski
{"title":"Recovery of chemical components from Spondias mombin L. leaves using pressurized hot water, ultrasound and turbo-extraction techniques.","authors":"Victor DE S Pereira, Diego Márlon Ferro, Janaína Carla B Machado, Magda Rhayanny A Ferreira, Luiz Alberto L Soares, Luiz Stragevitch, Leandro Danielski","doi":"10.1590/0001-3765202420240177","DOIUrl":"https://doi.org/10.1590/0001-3765202420240177","url":null,"abstract":"<p><p>The fruit of Spondias mombin (cajazeira) is widely consumed in the northeast region of Brazil. In this work, three different extraction methods, namely ultrasound-assisted extraction (UAE), turbo-extraction (TE) and pressurized hot water extraction (PHWE), were evaluated in order to investigate the potential of cajazeira leaves as an alternative source of bioactive compounds. The extraction methods were compared in terms of yield, chemical composition and total phenolic content (TPC) of the extracts. The highest yields and TPC values were obtained by TE with a mixture ethanol: water (70:30%, v/v) as the solvent. PHWE has not yet been applied for the extraction of cajazeira leaves. Thus, it was evaluated as one alternative for the recovery of phenolic compounds under conditions of 80, 100 and 120 °C and 100 and 120 bar. A modeling study of PHWE kinetics was investigated at 80 °C and 200 bar. Furthermore, a biorefinery approach considering integrated processes to recover bioactive compounds was investigated and the results showed that combining processes may enhance the valorization of agricultural waste.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240177"},"PeriodicalIF":1.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Alejandra Gallego, María Verónica Simoy, Darío Vezzani
{"title":"Citizen complaints in local media as source of mosquito data: the case of Aedes albifasciatus in an intermediate city of temperate Argentina.","authors":"María Alejandra Gallego, María Verónica Simoy, Darío Vezzani","doi":"10.1590/0001-3765202420240477","DOIUrl":"https://doi.org/10.1590/0001-3765202420240477","url":null,"abstract":"<p><p>Floodwater mosquitoes provoke nuisance due to their mass emergence. Citizen complaints about outstanding events of mosquitoes in urban areas are usually reflected in local media and could be potentially used as data. Our objective was to build a temperature dependent function to characterize the immature development time of the floodwater mosquito Ae. albifasciatus in Tandil (Argentina), and validate it using citizen complaints in local media and field collected mosquitoes. The development time obtained ranges from 5-47 days at 30-7°C, respectively. During 2000-2021, nine mosquito abundance peaks were identified through local journalistic records. For these events, the estimated hatching date was successfully associated with a previous precipitation, with a maximum difference of two days. The precipitation identified as the trigger for egg hatching ranged from 15 to 121 mm. Then, to associate mosquito news with the target species, female mosquitoes were captured by a battery-powered aspirator in green areas of the city during September 2019-June 2021. Six mosquito abundance peaks were detected in which Ae. albifasciatus accounted for 97.3% of the captures. Among them, journalistic records were able to detect the largest two; i.e. in our study above 15 mosquitoes in a five minute collection. Citizen complaints related to nuisance or vector species could be valuable retrospective data for researchers of different fields.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240477"},"PeriodicalIF":1.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabel Cristina T Proença, Patrick Türck, Vanessa D Ortiz, Cristina C Proença, Adriane Belló-Klein, Alexandre L DE Castro, Caroline Dani, Alex Sander R Araujo
{"title":"Treatment with thyroid hormones and grape juice in a model of pulmonary hypertension: the response of apoptosis and inflammation.","authors":"Isabel Cristina T Proença, Patrick Türck, Vanessa D Ortiz, Cristina C Proença, Adriane Belló-Klein, Alexandre L DE Castro, Caroline Dani, Alex Sander R Araujo","doi":"10.1590/0001-3765202420240338","DOIUrl":"https://doi.org/10.1590/0001-3765202420240338","url":null,"abstract":"<p><p>This study explored the association of cardioprotective role of thyroid hormones (TH) and benefit effects of grape juice (GJ) on vascular function against the apoptosis and inflammation associated with monocrotaline (MCT)-induced pulmonary hypertension (PH). Wistar rats were distributed into five groups: control; PH (MCT 60mg/kg i.p.); PH+GJ (GJ-7 µL/g / day, gavage for 14 days); PH+TH (T3, 2μg/100g/day, and T4, 8μg/100g/day, by gavage for 14 days); and PH+TH+GJ. Echocardiographic, morphometric measurements and expression of proteins associated with apoptosis and inflammation were evaluated. The reduced cardiac output (35%) in PH was attenuated in the PH+GJ, PH+TH, and PH+TH+GJ groups (P<0.05). Both GJ and TH had no effect on the PH-induced hypertrophy index. The PH + TH group showed reduced BAX (~90%) and increased Bcl2 levels (~80%) as compared to PH group (P<0.05). The PH-induced increase in caspase 3 was diminished (~92%) in the PH+TH (P<0.05). Both GJ and TH, isolated and combined, reduced caspase 9 levels (~70%). PH-provoked elevated NLRP3 levels was decreased (~90%) in PH+TH (P<0.05). The therapeutic approach showed cardioprotective effects in the PH model which may be mediated by mitigation of apoptosis and inflammation.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240338"},"PeriodicalIF":1.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}