Dárcia Gabriela B DA Costa, Daralyns B Macedo, Carlos Willian D Dantas, Regianne M Dos Santos Correa, Ádria D Dos Santos, Hendrya Julianny P Coelho, Carla Giovanni Á Moreira, Heden Luiz M Moreira, Rommel Thiago J Ramos, Marília Danyelle N Rodrigues
{"title":"Development of microsatellite markers for Leiarius marmoratus obtained by partial de novo genome assembly.","authors":"Dárcia Gabriela B DA Costa, Daralyns B Macedo, Carlos Willian D Dantas, Regianne M Dos Santos Correa, Ádria D Dos Santos, Hendrya Julianny P Coelho, Carla Giovanni Á Moreira, Heden Luiz M Moreira, Rommel Thiago J Ramos, Marília Danyelle N Rodrigues","doi":"10.1590/0001-3765202520241094","DOIUrl":"https://doi.org/10.1590/0001-3765202520241094","url":null,"abstract":"<p><p>This study addresses the identification of repetitive regions called microsatellites in the genome of the Amazonian species <italic>Leiarius marmoratus</italic>. An efficient methodology was used with sequencing and genome assembly to obtain and map specific markers. The programs MISA and EasySSR identified 221,273 and 145,601 markers, respectively. In addition, primers were developed, of which 590 were considered successful, according to in-silico testing with the Batchprime3 program. The results confirm the quality of the methodology, which can be applied in studies of genetic improvement, variability control, and genomic and comparative analyses.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97Suppl 2 Suppl 2","pages":"e20241094"},"PeriodicalIF":1.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pedro Henrique Cardoso, Fátima Regina G Salimena, Andrea P Luizi-Ponzo, Nádia Somavilla, Pâmela T Patrício, Marcelo Trovó
{"title":"Stachytarpheta serrana, a new species of Verbenaceae endemic to the Diamantina Plateau in Espinhaço Range (Minas Gerais, Brazil), with palynological and anatomical notes.","authors":"Pedro Henrique Cardoso, Fátima Regina G Salimena, Andrea P Luizi-Ponzo, Nádia Somavilla, Pâmela T Patrício, Marcelo Trovó","doi":"10.1590/0001-3765202520241271","DOIUrl":"https://doi.org/10.1590/0001-3765202520241271","url":null,"abstract":"<p><p>Stachytarpheta serrana, a new Brazilian species from the Serro municipality in the campos rupestres of the Diamantina Plateau, Minas Gerais state, is described and illustrated. Like most species of the genus found in this region, S. serrana has blue corollas and is morphologically related to S. glabra. Its recognition as a distinct species is supported by morphological, palynological, and anatomical evidence. The main traits for its recognition are its erect or suberect leaves, amphistomatic, with a foveolate abaxial surface and hirsute veins forming a reticulate network, flowers with a calyx 1.3-1.6 cm long and a corolla tube 2.3-2.6 cm long, subprolate pollen grains with a granulate colpi membrane, and fruits with a short beak at the apex. With a restricted distribution and known from only two recent collections, S. serrana should be classified as Data Deficient (DD).</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 suppl 2","pages":"e20241271"},"PeriodicalIF":1.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Júlia F Sant'ana, Emanuela Fernanda Tureta, Rafael L DA Rosa, Yohana P Calegari-Alves, Aline M Faustino, Ana Luiza Marques, Larissa Daniele Bobermin, André Quincozes-Santos, Ana Paula M Varela, Patrícia Sesterheim, Markus Berger, Ramon D Peña, Diogo O Souza, Paulo Roehe, Jorge A Guimarães, Alexandre R Campos, Lucélia Santi, Walter Orlando Beys-DA-Silva
{"title":"Zika virus infection in a non-neural cell host promotes differential expression of proteins associated with neurological conditions.","authors":"Júlia F Sant'ana, Emanuela Fernanda Tureta, Rafael L DA Rosa, Yohana P Calegari-Alves, Aline M Faustino, Ana Luiza Marques, Larissa Daniele Bobermin, André Quincozes-Santos, Ana Paula M Varela, Patrícia Sesterheim, Markus Berger, Ramon D Peña, Diogo O Souza, Paulo Roehe, Jorge A Guimarães, Alexandre R Campos, Lucélia Santi, Walter Orlando Beys-DA-Silva","doi":"10.1590/0001-3765202520240849","DOIUrl":"https://doi.org/10.1590/0001-3765202520240849","url":null,"abstract":"<p><p>The Zika Virus (ZIKV) is a Flavivirus that caused a recent outbreak worldwide resulting in different neurological outcomes that are still poorly characterized and understood. Concerning this issue, in vitro and in vivo models are being applied to improve the molecular understanding of ZIKV infection. In this work, applying shotgun proteomics we revealed the differential ZIKV infection proteome in Vero cells, a non-neural cell model. A dramatic change resulting from infection was found including the differential expression of several proteins previously associated with brain diseases. The molecular alterations caused by this pathogen were further characterized through bioinformatics such as Gene Ontology and protein-protein interaction network of resulting differential proteome. Our findings identified molecular markers that were differentially expressed during ZIKV infection and had been previously linked to neurological conditions and infections caused by ZIKV and/or SARS-CoV-2. The results presented in this article highlight molecular markers associated with neurological dysfunctions, demonstrating that ZIKV infection can dysregulate neural-specific genes, even in non-neural cells.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 suppl 2","pages":"e20240849"},"PeriodicalIF":1.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Regina G Bezerra, Fernanda Staniscuaski, Marcia Cristina B Barbosa
{"title":"Breaking the glass ceiling: women's perceptions of academic-scientific careers in physics and nursing.","authors":"Ana Regina G Bezerra, Fernanda Staniscuaski, Marcia Cristina B Barbosa","doi":"10.1590/0001-3765202520241497","DOIUrl":"10.1590/0001-3765202520241497","url":null,"abstract":"<p><p>Women's representation in science has grown, yet they still comprise only about a third of the global scientific community. While women are the majority in fields like social, biological, and health sciences, they remain underrepresented in others, such as exact sciences and engineering, from training stages to senior positions. Beyond gender-related challenges, motherhood introduces additional barriers. In this study, we analyzed the perceptions of research productivity fellows from the National Council for Scientific and Technological Development in the fields of physics and nursing regarding their academic-scientific trajectories. These fields present contrasting professional landscapes: women are a minority in physics and a majority in nursing. Data collected in 2020 from fellows active in 2019 revealed that being a woman and, in particular, being a mother impacts scientific careers differently across disciplines. In physics, participants perceived significant obstacles, including gender-based challenges and the disruption caused by motherhood. In nursing, however, motherhood was seen as more integrated into the academic journey. These findings underscore how the academic environment, shaped by the gender composition of a field, profoundly influences career experiences. Addressing these disparities requires context-specific strategies to create equitable and inclusive scientific environments.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241497"},"PeriodicalIF":1.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bianca M F Silva, Rafael M P Carvalho, Josiane R Domingues, Lucielen O Santos, Gustavo L P A Ramos, Alice G M Gonzalez
{"title":"Effect of pH and temperature on Shiga toxin-producing Escherichia coli O157:H7 isolated from bovine reservoir.","authors":"Bianca M F Silva, Rafael M P Carvalho, Josiane R Domingues, Lucielen O Santos, Gustavo L P A Ramos, Alice G M Gonzalez","doi":"10.1590/0001-3765202520241080","DOIUrl":"10.1590/0001-3765202520241080","url":null,"abstract":"<p><p>The aim of this study was to evaluate the individual and combined effect of pH (between 4.0 and 7.0) and storage temperature (between 6 and 35°C) on two wild-type <italic>E. coli</italic> O157:H7 (STEC 902 and STEC 2004) strains. The experiments were performed in laboratory culture medium, employing Central Composite Rotational Design (CCRD). For comparison, Enteropathogenic <italic>E. coli</italic> (EPEC) O55:H7 and non-pathogenic <italic>E. coli</italic> (npEC) strains were used. To validate the model, seven additional tests not included in the experimental design were performed. The model showed goodness-of-fit. The CCRD results indicated that the temperature significantly affected the microbial population of <italic>E. coli</italic> strains studied. With best development at temperatures between 20 and 35°C, while refrigeration temperatures (between 6 and 10°C) are unfavorable for the development of STEC O157:H7, independent of pH. The wild-type STEC 902 and STEC 2004 strains showed similar behavior under pH and temperature conditions in laboratory culture medium. On the other hand, control strains showed different behavior than STEC strains, being significantly affected by pH. The data indicate that the response to stress resulting from exposure to different pH and temperature levels varies according to the <italic>E. coli</italic> strain evaluated.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241080"},"PeriodicalIF":1.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcos Sidney B Oliveira, André M Alves, Elvis S Lima, Edson A Adriano, Marcos Tavares-Dias
{"title":"Isonebula clavuslongus n. sp. (Isopoda: Cymothoidae) parasitizing Myloplus ternetzi (Norman, 1929), a fish of the family Serrasalmidae, in the Brazilian Amazon region.","authors":"Marcos Sidney B Oliveira, André M Alves, Elvis S Lima, Edson A Adriano, Marcos Tavares-Dias","doi":"10.1590/0001-3765202520240590","DOIUrl":"https://doi.org/10.1590/0001-3765202520240590","url":null,"abstract":"<p><p>Crustaceans of the family Cymothoidae are important components of the invertebrate fauna of marine and freshwater environments. The genus Isonebula Taberner, 1977, has only two described species (Isonebula maculatus (Taberner, 1977) and Isonebula acanthopleon Taberner 1998), which are both parasites of freshwater fish in South America. During a field survey of fish parasites in the Araguari River, state of Amapá, Brazilian Amazon region, we found a new species of the genus Isonebula infesting the mouth (on the host's tongue) of Myloplus ternetzi (Norman, 1929), a characiform of the family Serrasalmidae, for which we proposed the name Isonebula clavuslongus n. sp. Isonebula clavuslongus n. sp. differs from its congeneric species through the larger size of the dactyl of its 6th pereopod, with an inner measurement of 4.50 mm. Additionally, it possesses 7 joints in the second antenna, whereas 9 joints were reported for I. acanthopleon and I. maculatus. The present study thus expands the knowledge of the diversity of Cymothoidae in the Amazon region to 26 species, and of the genus Isonebula, with 3 species, which stands out as one of the genera with the lowest number of known species among Neotropical Cymothoidae that parasitize freshwater fish.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240590"},"PeriodicalIF":1.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniela S Ferreira, Erlediel G DO Nascimento, Helen Maria P Sotão, Josiane S Monteiro
{"title":"Revisiting Diplococcium peruamazonicum Matsush. (Ascomycota) 31 years after its description.","authors":"Daniela S Ferreira, Erlediel G DO Nascimento, Helen Maria P Sotão, Josiane S Monteiro","doi":"10.1590/0001-3765202520241172","DOIUrl":"10.1590/0001-3765202520241172","url":null,"abstract":"<p><p>Diplococcium peruamazonicum Matsush. (Helotiales, Ascomycota) is being reported for the first time from Brazil and for the second time worldwide, 31 years after its original description in Peru. This rare ascomycete was found on Marajó Island (Brazilian Amazon) as a saprobic fungus on leaf litter of Astrocaryum vulgare Mart. A detailed morphological description, illustrations and taxonomic comments are provided here, as well as a key to the Diplococcium species recorded in Brazil. This report expands the geographic distribution of D. peruamazonicum in the Amazon.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241172"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Syeda Aleena Ubaid, Hafiz Muhammad Usman Abid, Muhammad Hanif, Khalid Mahmood, Memoona Qayyum, Nabeela Ameer
{"title":"Chitosan/Maleic anhydride and Itaconic acid super porous hydrogels, containing levofloxacin for the treatment of visceral bleeding.","authors":"Syeda Aleena Ubaid, Hafiz Muhammad Usman Abid, Muhammad Hanif, Khalid Mahmood, Memoona Qayyum, Nabeela Ameer","doi":"10.1590/0001-3765202520240463","DOIUrl":"https://doi.org/10.1590/0001-3765202520240463","url":null,"abstract":"<p><p>Visceral bleeding poses a significant threat, requiring effective management through positively charged chitosan. This study aimed to address the limitations of chitosan-based hemostatic hydrogels by synthesizing a modified maleic anhydride complex of chitosan (MAC). Biocompatibility, biodegradability, and non-toxicity made MAC a suitable candidate for super porous hydrogels (SPHs) used in hemostatic applications. Graft polymerization synthesized MAC, confirmed via Fourier transform infrared spectroscopy, demonstrating superior antioxidant activity compared to chitosan and maleic anhydride. SPHs were prepared using MAC alone and with N, N-methylene bisacrylamide (MBA) as crosslinkers. MAC exhibited higher water absorbency and pH-dependent swelling than the MBA-chitosan complex. Drug loading and entrapment efficiency varied with the drug-to-SPH ratio. In vitro drug release studies showed sustained release (SR) with SPHs-MAC following zero-order kinetics and SPHs-MBA following Korsmeyer-Peppas. SPHs-MAC gelled more rapidly with temperature changes, and MAC hydrogels showed larger antimicrobial zones of inhibition (28±2).In vivo hemostasis studies revealed rapid hemostatic effects with MACL-H, reducing blood loss to 78.5 ± 1.5 mg in iver rat models. MAC hydrogels significantly lowered blood loss in rat tail amputation models compared to controls. This formulation demonstrates strong potential for effective visceral bleeding management.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240463"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lin Wang, Guojian Shao, Jingbo Su, Yang Sun, Dawei Guan, Yuebo Fan, Sen Yao, Chunyu Li
{"title":"Three-dimensional calculation methods on global stability safety factor of diaphragm wall slurry trench based on minimum potential energy principle.","authors":"Lin Wang, Guojian Shao, Jingbo Su, Yang Sun, Dawei Guan, Yuebo Fan, Sen Yao, Chunyu Li","doi":"10.1590/0001-3765202520240335","DOIUrl":"https://doi.org/10.1590/0001-3765202520240335","url":null,"abstract":"<p><p>Due to the unreasonable assumptions for the three-dimensional (3D) sliding surface, the calculated results of the global stability safety factor (GSF) of the diaphragm wall slurry trench are often excessively large. In order to improve this phenomenon, the 3D calculation model on the GSF of trench wall is established by assuming the sliding surface to be a quarter ellipsoidal surface and the sliding mass to be a quarter ellipsoid. Then the 3D energy method is proposed based on the minimum potential energy principle, and further the 3D equivalent energy method is also come up with adopting the equivalent trench length to replace the actual trench length. The correctness of the models established and the rationality of the calculation methods proposed are verified based on ten engineering cases and the sensitivity of the GSF to various parameters is analyzed on the basis of seven design examples. Finally, the different calculation methods are used to check the global stability of the slurry trenches in practical engineering, the results prove that the calculation methods presented are rationality once again and the 3D equivalent energy method has better engineering applicability.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240335"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jocimar C Milagre, Lucas José Mendes, Pedro S DA Silva, William Renato T Dos Santos, Guilherme Diego Fockink, Fabiane Granzotto, Agnes Estela Fontana, Ricardo B Schenato, Ana Paula M Rovedder
{"title":"Long-term effects of cattle rearing on vegetation and soil in riparian forests in southern Brazil.","authors":"Jocimar C Milagre, Lucas José Mendes, Pedro S DA Silva, William Renato T Dos Santos, Guilherme Diego Fockink, Fabiane Granzotto, Agnes Estela Fontana, Ricardo B Schenato, Ana Paula M Rovedder","doi":"10.1590/0001-3765202520240333","DOIUrl":"https://doi.org/10.1590/0001-3765202520240333","url":null,"abstract":"<p><p>Comprehending the impacts of the presence of cattle on different compartments of riparian forests is essential for the management of these environments, which play a crucial role in biodiversity conservation and human well-being. The objective of this study was to assess the long-term effects of cattle on the structure (tree stratum and natural regeneration) and floristic composition of vegetation, as well as on the litter and soil carbon stock (0-5, 5-15, 15-30, and 30-100 cm) in riparian forests. We compared two forest areas in southern Brazil, one isolated from cattle for 28 years and another used by cattle with a high animal load. Our results indicate that cattle modify the floristic composition and structure of vegetation, mainly affecting litter carbon stock and the abundance, richness, and diversity of natural regeneration. However, we did not observe a significant change in soil carbon stock and its correlation with vegetation variables. Our findings emphasize the need to explore adaptation alternatives of livestock activities with the maintenance of riparian forest ecosystem services. Adequate cattle load management and fencing of riparian forests, with the installation of watering corridors for cattle, are essential actions to reconcile livestock production with forest conservation.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240333"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}