David M Unwin, Roy E Smith, Samuel L A Cooper, David M Martill
{"title":"Reinterpretation of Bakiribu waridza from the Romualdo Formation (Lower Cretaceous) of Brazil: a fish not a pterosaur.","authors":"David M Unwin, Roy E Smith, Samuel L A Cooper, David M Martill","doi":"10.1590/0001-3765202620251374","DOIUrl":"https://doi.org/10.1590/0001-3765202620251374","url":null,"abstract":"<p><p>Fragmentary fossil remains from the Romualdo Formation (Lower Cretaceous) of Brazil, preserved in association with two fish were interpreted as two individuals of a new genus and species of ctenochasmatine pterosaur, Bakiribu waridza Pêgas et al. 2025. Comparison with fossils from the same geological unit show that the remains represent the gill arch apparatus of a large actinopterygian fish. A series of partially articulated bony elements correspond closely in morphology to the ceratohyals, hypohyals, basibranchial and hypobranchials of amiid fish while the purported 'teeth', which lack enamel, dentine tubules and in most cases evidence of a structure corresponding to a pulp cavity, are reinterpreted as gill filaments. Bakiribu waridza is a fish, not a pterosaur and the name, founded on indeterminate remains of an actinopterygian fish, possibly an amiid, should be treated as a nomen dubium. Rather than a regurgitalite this association of several fish remains appears to be a typical Romualdo Formation concretion. This reinterpretation of Bakiribu has negligible impact on our current understanding of the evolutionary history of pterosaurs as ctenochasmatines represented, for example, by Pterodaustro, were already known to be present in South America until the end of the Early Cretaceous.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 2","pages":"e20251374"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrícia Alessandra M M Donicht, Ricardo Z Vaz, Leonir Luiz Pascoal, Fabiano N Vaz, Dari Celestino Alves Filho, João Restle
{"title":"Fat thickness, conformation, carcass weight, and age on the physical composition and quality of the meat from Nellore cows.","authors":"Patrícia Alessandra M M Donicht, Ricardo Z Vaz, Leonir Luiz Pascoal, Fabiano N Vaz, Dari Celestino Alves Filho, João Restle","doi":"10.1590/0001-3765202620240765","DOIUrl":"https://doi.org/10.1590/0001-3765202620240765","url":null,"abstract":"<p><p>The carcasses of cull cows are normally penalized and paid only for their weight and age, without considering muscle expression, subcutaneous fat and marbling. The aim of this study was to analyse the effects of carcass subcutaneous fat thickness (SFT), conformation score, cold carcass weight (CCW), and age on the physical composition of the carcass and the meat quality of 152 Nellore cull cows, aged between 3 and 16 years. The data were submitted to regression analysis by Proc Reg and Proc Stepwise procedures. An increase of 1 kg in CCW and one point in the conformation score resulted in increases of 0.12 kg and 1.70 kg in muscle weight, respectively. SFT and age had a positive effect on the percentage of fatty tissue (0,56 and 0,23%, respectively), and a negative effect on the percentage of muscle tissue (0,47 and 0,23%, respectively). Intramuscular fat increased every 0.11 mm of SFT and every 0.21 points in carcass conformation. The lowest shear force occurred SFT reached 5.6 mm. Meat palatability increased until SFT reached 7.1 mm. Quality of meat from Nellore cows, occurs with increased weight and carcass conformation, reducing age at slaughter and SFT between 5 and 8 mm.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20240765"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Nickel by Gavage and Intraperitoneal Injection on Histomorphology, Nutrient Metabolism, Major and Trace Elements in Mice.","authors":"Aifei DU, Wei Luo, Lulu Yang, Huijuan Zhao, Yinping Li, Tingrui Bai, Shaohua Feng, Shibin Yuan, Bangyuan Wu","doi":"10.1590/0001-3765202620250190","DOIUrl":"https://doi.org/10.1590/0001-3765202620250190","url":null,"abstract":"<p><p>Nickel (Ni), an essential trace element, poses growing environmental and health risks due to industrial activities. This study evaluated nickel toxicity in mice via gavage and intraperitoneal injection, analyzing(i) histopathological damage, (ii) macronutrient metabolism, assessed by crude protein (CP) and crude fat (CF) contents and (iii) the tissue distribution of trace and major elements. Histopathological injuries were assessed via H&E staining, while CP and CF levels were quantified using the Kjeldahl method and Soxhlet extractor method. Flame atomic absorption spectrometry measured Ni, Calcium (Ca), Manganese (Mn), Magnesium (Mg), Copper (Cu), Zinc (Zn), and Ferrum (Fe) concentrations. Both administration routes induced dose-dependent damage to the duodenum, jejunum, ileum, liver, lungs, and kidneys. Gavage primarily injured the small intestine and liver, whereas intraperitoneal injection predominantly affected the liver and kidneys. Intestinal villus/crypt values (V/C), CP, and CF decreased significantly with rising nickel doses (P<0.05). Tissue nickel accumulation increased proportionally with exposure levels, accompanied by reduced Fe and Cu concentrations and elevated Zn in specific organs. Ca, Mn, and Mg levels remained stable across treatments.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20250190"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maikow Zago, Isaac S Nunes, Daiane Skupin DA Silva, Núbia Cristina W Freitas, Dienefer Frizzo Junker
{"title":"Treatment of wastewater containing fuchsin by electro-oxidation / photo-electro-cupro-fenton processes.","authors":"Maikow Zago, Isaac S Nunes, Daiane Skupin DA Silva, Núbia Cristina W Freitas, Dienefer Frizzo Junker","doi":"10.1590/0001-3765202620250059","DOIUrl":"https://doi.org/10.1590/0001-3765202620250059","url":null,"abstract":"<p><p>In this work, the electro-oxidation process based on reactions with copper (cathode) and graphite (anode) electrodes, referred to in the literature as Cupro Fenton, was applied to the degradation of basic fuchsin. The real wastewater used was generated in a microbiology laboratory from practical classes, research projects, and course completion work at a university in the northwest of Rio Grande do Sul, Brazil. The results demonstrate that electro-oxidation as a standalone process is insufficient in removing the contaminant, which consequently necessitated the use of UVC light and H₂O₂. The electro-oxidation and electro-cupro Fenton processes were complementarily applied to remove the Basic Fuchsin dye (FCS-B) from the wastewater sample. Experiments were conducted at pH values of 5, 7, and 9, and current densities of 3, 6, and 9 mA cm⁻². Current density was identified as a significant parameter (p = 0.0178). The application of electro-oxidation did not satisfactorily remove the contaminant, and H2O2 and 254 nm UVC light were used as an additional source of ●OH, achieving removal rates 88.61% for COD, 86.52% for fuchsin, and 98.34% for color. Ecotoxicological tests using allium cepa suggested the presence of residual H2O2, which requires careful consideration regarding the possibility of direct environmental disposal.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20250059"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Valtemar F Cardoso, Caio C DE Souza, Juliane C Glória, Eliza Raquel D DA Silva, Ariamna María Dip Gandarilla, Walter Ricardo Brito, Luis André M Mariúba
{"title":"Molecular assays and point-of-care tools: a review of amplification methods and nucleic acid detection systems.","authors":"Valtemar F Cardoso, Caio C DE Souza, Juliane C Glória, Eliza Raquel D DA Silva, Ariamna María Dip Gandarilla, Walter Ricardo Brito, Luis André M Mariúba","doi":"10.1590/0001-3765202620250565","DOIUrl":"https://doi.org/10.1590/0001-3765202620250565","url":null,"abstract":"<p><p>Molecular diagnosis is crucial for combating infectious diseases due to its high sensitivity and precision. However, its application in point-of-care testing (POCT) faces challenges, particularly in remote areas and developing countries. This review, based on searches of indexed publication databases, examined major nucleic acid amplification methods, detection techniques and miniaturization strategies for POCT platform development, focusing on the period 2015-2025. The need for regulatory standardization and the advantages and disadvantages of amplification techniques were discussed. Microfluidic approaches-reaction chambers, continuous flow, and droplets-were analyzed as the basis for functional device integration, emphasizing their role in reducing volumes, energy consumption and processing time. The sensitivity, cost and instrumentation complexity of fluorescence-, colorimetry- and electrochemistry-based detection systems were addressed. The practical feasibility of integrating these into miniaturized POCT platforms underscores the structural role of microfluidics in advancing portable molecular diagnostics. Despite progress, miniaturization complexity and reagent costs remain significant technological barriers.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20250565"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angelo Cezar Bolognese Junior, Mariana Navarro, Ana Luiza R Duarte, Gabriela B V DA Silva, Anne Caroline S Rosa, Ghiovani Z Raniero, Antônio Roberto G Monteiro, Roberto K N Cuman, Maria Raquel M Natali
{"title":"Non-celiac wheat gluten sensitivity model (NCGS): evaluation of gastric and intestinal mucosal cells in Wistar rats.","authors":"Angelo Cezar Bolognese Junior, Mariana Navarro, Ana Luiza R Duarte, Gabriela B V DA Silva, Anne Caroline S Rosa, Ghiovani Z Raniero, Antônio Roberto G Monteiro, Roberto K N Cuman, Maria Raquel M Natali","doi":"10.1590/0001-3765202620250688","DOIUrl":"https://doi.org/10.1590/0001-3765202620250688","url":null,"abstract":"<p><p>Non-celiac gluten sensitivity is an enteropathy triggered by ingestion of gluten-containing foods. The aim of this study was to evaluate effects of diets with increasing concentrations of wheat gluten on the gastric and intestinal mucosae of male Wistar rats. A total of 21 animals were used, distributed into three experimental groups according to percentage of gluten in the diet: G14 (14%, control), G42 (42%), and G70 (70%), fed for 100 days. Results showed diets with higher gluten content significantly increased final body mass, elevated serum triglyceride levels, and enhanced myeloperoxidase activity, indicating systemic inflammatory response. Histologically, no significant changes were observed in the number of gastric mucosal cells. However, a significant increase in intraepithelial lymphocytes was observed in duodenum of G42 and G70 groups, along with qualitative increase in inflammatory aggregates in lamina propria and higher incidence of ileal Peyer's patches. Data indicates prolonged exposure to gluten-rich diets may trigger inflammatory changes in intestinal mucosa, even in absence of celiac disease, without compromising gastric mucosa. It is concluded this condition may be associated with adaptive and inflammatory intestinal responses dependent on gluten concentration in diet.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20250688"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ione Maria Mendes, Vanessa O Fagundes, Luisa Massarani, Yurij Castelfranchi, Ildeu C Moreira, Carmelo Polino, Adriana B DE Carvalho
{"title":"Effects of age on the perception of science and technology: a comparative study of two Brazilian surveys.","authors":"Ione Maria Mendes, Vanessa O Fagundes, Luisa Massarani, Yurij Castelfranchi, Ildeu C Moreira, Carmelo Polino, Adriana B DE Carvalho","doi":"10.1590/0001-3765202620250138","DOIUrl":"https://doi.org/10.1590/0001-3765202620250138","url":null,"abstract":"<p><p>Indicators of the public perception of science and technology (S&T) are essential for strategic decision-making, providing input for closer exchange between science and diverse audiences. Our article aims to investigate the effects of age on interest, opinions, and attitudes regarding science and technology, comparing the results of two surveys with representative samples of the young and adult Brazilian population. The results show that the two audiences have positive views and high interest in S&T, but that they rarely visit spaces dedicated to cultural scientific activities. Young Brazilians expressed greater interest than adults in S&T. Even with less explicit interest in S&T and less contact with cultural scientific activities, adults were more likely to support more funding for S&T in Brazil. Whether for young people or adults, regional aspects should be considered in the perception and public communication of science and technology in Brazil to establish more assertive activities.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20250138"},"PeriodicalIF":1.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wallace N DE Jesus, Maria Eduarda A Almeida, Maria Clara S Ramos, Karine B Machado, Thiago L Rocha, Luciana Damacena-Silva
{"title":"Ecotoxicity of polyhexamethylene biguanide hydrochloride (PHMB) in the neotropical snail Biomphalaria glabrata (Say, 1818).","authors":"Wallace N DE Jesus, Maria Eduarda A Almeida, Maria Clara S Ramos, Karine B Machado, Thiago L Rocha, Luciana Damacena-Silva","doi":"10.1590/0001-3765202620241099","DOIUrl":"https://doi.org/10.1590/0001-3765202620241099","url":null,"abstract":"<p><p>The polyhexamethylene biguanide hydrochloride (PHMB) is a sanitizer with a broad range of potential applications. Nevertheless, the ecotoxicity of PHMB to gastropod snails remains scarce. Thus, the current study aimed to assess whether the exposure of freshwater snails Biomphalaria glabrata to PHMB induces changes in biometric and behavioral parameters, cellular immune responses, and mutagenicity. Snails were exposed to PHMB at 0.4, 0.6 and 0.8 mg L-1 for 120 h. Shell size and total weight of the snails were measured. Lethargy, shell reclusion, lateral tilt and hemolymph release behavior were evaluated. Hemocyte counts and frequencies of micronuclei, binucleated cells, and apoptotic cells were also assessed. PHMB had no effect on shell diameter or total weight of snails; however, PHMB at 0.8 mg L⁻¹ induced severe behavioral changes such as visceral mass exposure, hemolymph loss, and death within 24 hours. PHMB reduced the frequency of granulocyte hemocytes and increased the frequency of hyalinocytes at 0.8 mg L-1 and blast-like cells at 0.4 mg L-1. Mutagenic effects were observed in snails exposed to PHMB at 0.6 and 0.8 mg L⁻¹, with increased frequencies of micronuclei, binucleated cells and apoptotic cells. PHMB induced behavioral, immunotoxic, and mutagenic changes in B. glabrata, demonstrating its potential ecotoxicological impact to freshwater gastropods.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20241099"},"PeriodicalIF":1.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Florencia Colla, Pablo Agustín Collins, María Eugenia Farías
{"title":"Life at the extreme: aquatic invertebrates across environmental gradients in high altitude saline lakes of Central Andes (Argentina).","authors":"María Florencia Colla, Pablo Agustín Collins, María Eugenia Farías","doi":"10.1590/0001-3765202620241226","DOIUrl":"https://doi.org/10.1590/0001-3765202620241226","url":null,"abstract":"<p><p>This study aimed to determine main community attributes -occurrence, abundance and diversity indices- of zooplanktonic and benthic invertebrates in ten saline lakes located between 3,009 and 4,577 masl in Central Andes (Argentina), and to analyze how environmental variables affect these attributes using multivariate techniques. Sampling campaigns were performed during the spring-summer season (October to April), between 2016 and 2018. The ordination techniques classified the lakes into low- to high-altitude, and hypo- to hyperhaline groups. A total of fifteen taxa of invertebrates were recorded (seven planktonic and eight benthic) and the assemblages presented low abundance, low species richness (S), low diversity (Shannon index, H) and high dominance (Simpson index, D). Zooplanktonic and benthic community attributes were highly variable across environmental gradients, with a general trend to exhibit higher taxa richness at lower salinity content. Altitude and conductivity partially explained the spatial distribution of the assemblages, with some taxa only found in high altitude lakes (Cladocera) or hyperhaline lakes (Ephydridae), whereas others were widely distributed across the gradients (harpacticoids copepods, Monhysteridae nematods). Further research conducted at a smaller intra-lake scale may contribute baseline ecological information on the biota of these fragile and yet understudied systems, currently exposed to increasing anthropogenic pressures.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20241226"},"PeriodicalIF":1.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taiana S Matos, Carlos Alberto B DA Silva Junior, Marcos Vinícius S Paula, Severino Alves Júnior, Alisson Clay R DA Silva, Verônica S Candido
{"title":"Polyvinyl alcohol /chitosan membranes with copaiba oil emulsion and silver nanoparticles for possible application in wounds.","authors":"Taiana S Matos, Carlos Alberto B DA Silva Junior, Marcos Vinícius S Paula, Severino Alves Júnior, Alisson Clay R DA Silva, Verônica S Candido","doi":"10.1590/0001-3765202620250065","DOIUrl":"https://doi.org/10.1590/0001-3765202620250065","url":null,"abstract":"<p><p>Research on new materials for wound care plays a key role in advancing biomaterials. The present study focused on the elaboration and characterization of polyvinyl alcohol /chitosan (BP) membranes in a ratio of 0.2/0.8 (m/m), with the addition of copaiba oil emulsion and silver nanoparticles (AgNPs), using the casting technique. The nanoparticles were evaluated by UV/Vis spectroscopy and Transmission Electron Microscopy (TEM), while the films were analyzed by Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy, and swelling, humidity, and contact angle. UV/Vis findings exhibited the characteristic absorption of AgNPs. The films containing 0.5% copaiba oil emulsion exhibited rough surfaces and few droplets. FTIR spectra indicated the presence of caryophyllene in the membranes at 860 cm-1. The samples demonstrated high fluid absorption capacity, with the BP membrane presenting the highest average hydrophilic contact angle (74.53° in PBS). The humidity content of the BP with 0.5 mL of copaiba oil and AgNPs (BP/AgNPs-0.5) membrane was considerable, with an average of 90.24 %. The results indicate that the BP/AgNPs-0.5 films have great potential for wound care applications.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 1","pages":"e20250065"},"PeriodicalIF":1.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}