Anais da Academia Brasileira de Ciencias最新文献

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Effect of microencapsulation on antioxidant activities of Eugenia punicifolia (Kunth) DC hydroethanolic extracts. 微胶囊对 Eugenia punicifolia (Kunth) DC 水乙醇提取物抗氧化活性的影响。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240184
Manoela Martins, Danijela Stanisic, Catarina Dos Santos
{"title":"Effect of microencapsulation on antioxidant activities of Eugenia punicifolia (Kunth) DC hydroethanolic extracts.","authors":"Manoela Martins, Danijela Stanisic, Catarina Dos Santos","doi":"10.1590/0001-3765202420240184","DOIUrl":"https://doi.org/10.1590/0001-3765202420240184","url":null,"abstract":"<p><p>Eugenia punicifolia (Kunth) DC (Myrtaceae) is a folk medicinal plant in the Brazilian Cerrado with antioxidant, anti-inflammatory, antinociceptive, antiulcerogenic activities, etc., usually attributed to its phenolic compounds. Since these compounds are sensitive to heat and light, and to increase their applications, Hydroethanolic Extracts E. punicifolia (HEEP, EtOH:H2O 70% v/v) were encapsulated by freeze-drying in xanthan gum (mesh 80, HEEPX80; mesh 200, HEEPX200) in ratio 1:1(w/w). Flavonoids had the highest encapsulation efficiency in HEEPX80, with a total flavonoid content of 55.56%. The release profile at different pH levels showed that pH = 4.5, a relevant antioxidant activity for HEEPX80 and HEEPX200. Also, in HEEP-modified release, higher antioxidant activity was observed in more acidic media (pH 4.5) than in a more neutral medium (pH 7.4). From these results, we could infer that HEEP encapsulations with Xanthan gum could be a good alternative for preserving antioxidants in these extracts.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240184"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Disinfection of indoor air using ultraviolet and titanium dioxide photocatalytic reactor. 利用紫外线和二氧化钛光催化反应器对室内空气进行消毒。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240304
Bruna B Vargas, Adriane A L Rodriguez, Camila Crauss, Carolina V Barbosa, Carine Baggiotto, Ênio Leandro Machado, Vanessa Rosana Ribeiro
{"title":"Disinfection of indoor air using ultraviolet and titanium dioxide photocatalytic reactor.","authors":"Bruna B Vargas, Adriane A L Rodriguez, Camila Crauss, Carolina V Barbosa, Carine Baggiotto, Ênio Leandro Machado, Vanessa Rosana Ribeiro","doi":"10.1590/0001-3765202420240304","DOIUrl":"https://doi.org/10.1590/0001-3765202420240304","url":null,"abstract":"<p><p>Air contamination in confined environments can lead to severe health damage. Searching for effective and sustainable technologies that might bring quality to indoor air is necessary. Heterogeneous photocatalysis has been studied for its ability to oxidize, inactivating microorganisms in the air. In the present work, a reactor was assembled, where titanium dioxide (TiO2) P25 was incorporated into the inner face of polyvinyl chloride (PVC) tubes and vegetable sponges (Luffa sp.). Polyester Orthophthalic (PO) resin was used to fix the TiO2 onto the surfaces. Ultraviolet lamps (UVA) were used to activate the TiO2 catalyst to test the inactivation capacity of microorganisms, as they are economical and present high energy efficiency and long service life. The inactivation of microorganisms was evaluated in natural and artificially contaminated atmospheres. The photocatalytic reactor proved efficient in most tests in both atmospheres. In tests 1 and 2, no bacterial colony-forming units (CFUs) were found in the photocatalysis tube. In test 3, the average of 5 CFUs of fungi in the photocatalysis tube and 12.67 in the control tube was found, indicating inactivation. Therefore, this research is essential for presenting an alternative solution for indoor air treatment.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240304"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Removal of antibiotics in constructed wetlands: a review and bibliometric analysis. 在人工湿地中去除抗生素:综述和文献计量分析。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240275
Valdemir F DA Silva, Delmira Beatriz Wolff
{"title":"Removal of antibiotics in constructed wetlands: a review and bibliometric analysis.","authors":"Valdemir F DA Silva, Delmira Beatriz Wolff","doi":"10.1590/0001-3765202420240275","DOIUrl":"https://doi.org/10.1590/0001-3765202420240275","url":null,"abstract":"<p><p>This study offers a comprehensive systematic review on the removal of antibiotics in Constructed Wetlands (CWs), evaluating their efficacy as an alternative to conventional wastewater treatment methods. Data from 82 articles were analyzed, focusing on removal rates, antibiotic types, and system configurations, including scale, vegetation, sediment, flow, hydraulic retention time (HRT), and hydraulic loading rate (HLR). The findings indicate that full-scale CWs, particularly those utilizing vegetation like Cyperus alternifolius L. and materials such as shells, zeolites, medicinal stone, and ceramics, achieved removal rates exceeding 90% for various antibiotics. The study underscores the importance of optimizing both anaerobic and aerobic conditions to enhance removal efficiency, noting that aerobic environments promote oxidative processes effective for β-lactams, while anaerobic environments facilitate reduction processes beneficial for sulfonamides. Hybrid systems combining these zones demonstrated maximal removal efficiency. This review advances the understanding of antibiotic removal in CWs, presenting them as a viable, sustainable solution to mitigate environmental impacts, preserve water quality, and protect public health. Further research is recommended to explore the impact of root types and bed configurations on removal efficiency.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240275"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Petrographic and Petrophysical Characterization of Sandstones from Rio Bonito Formation, Paraná Basin (Southern Brazil). 巴拉那盆地(巴西南部)里奥博尼托地层砂岩的岩石学和岩石物理学特征。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-11 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240365
Paulo Frederico O Ramos, Giovanni C Stael, Rodrigo B V Azeredo, Marcus Vinicius B Ade, Sérgio Bergamaschi, Juarez Lourenço, Silvia Lorena B Bermudez
{"title":"Petrographic and Petrophysical Characterization of Sandstones from Rio Bonito Formation, Paraná Basin (Southern Brazil).","authors":"Paulo Frederico O Ramos, Giovanni C Stael, Rodrigo B V Azeredo, Marcus Vinicius B Ade, Sérgio Bergamaschi, Juarez Lourenço, Silvia Lorena B Bermudez","doi":"10.1590/0001-3765202420240365","DOIUrl":"https://doi.org/10.1590/0001-3765202420240365","url":null,"abstract":"<p><p>This study focuses on the laboratory-scale petrophysical characterization of sandstones from the Rio Bonito Formation (Lower Permian), Paraná Basin, carried out from drill core samples from well PN-14-SC.02 obtained by CPRM-Brazilian Geological Service in the 1980s on the eastern edge of the Basin. This study includes integrating experimental data from routine petrophysics, sedimentary petrography, and nuclear magnetic resonance (NMR) obtained from 6 samples arranged in the normal direction of the stratification, present between 40 and 200 m deep. It was possible to conclude that the values of the permeability and porosity properties obtained from the NMR technique correlated in a very satisfactory way, with correlation coefficient R2 = 0.957 and Root Mean Squared Error (RMSE) = 0.208 about the porosity reference results offered by routine petrophysics, with lower values being less than a porosity unit (+/- 1 p.u.), in the range between 8 and 14%. The same was observed for the estimated permeability, R2 = 0.885 and RMSE = 0.152, by the Timur Coates method, with values in the range between 0.096 and 2.42 mD, which were well supported by the spectra information, as well as by petrographic analyses.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240365"},"PeriodicalIF":1.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First record of Dermanura gnoma for the threatened Pernambuco Endemism Center, northern Atlantic Forest, with phylogenetic insights. 大西洋森林北部伯南布哥特有物种中心濒危物种 Dermanura gnoma 的首次记录及系统发育见解。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-11 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240102
Camila F Gonçalves, Patrício A Rocha, Mayara G Beltrão, Ana P Carmignotto, Pedro M Galetti
{"title":"First record of Dermanura gnoma for the threatened Pernambuco Endemism Center, northern Atlantic Forest, with phylogenetic insights.","authors":"Camila F Gonçalves, Patrício A Rocha, Mayara G Beltrão, Ana P Carmignotto, Pedro M Galetti","doi":"10.1590/0001-3765202420240102","DOIUrl":"https://doi.org/10.1590/0001-3765202420240102","url":null,"abstract":"<p><p>Dermanura gnoma (Handley, 1987) is a phyllostomid bat that occurs in South America. In Brazil, most of the records are concentrated in the Amazon Forest, with sparse data from Cerrado and Atlantic Forest. This study presents the first record of D. gnoma for Alagoas state, and for the Pernambuco Endemism Center (PEC), the most threatened region of the Brazilian Atlantic Forest, characterized by its high rate of endemism, habitat loss, and fragmentation. The species was surveyed at Murici Ecological Station and its identification was supported by morphological and molecular data. We provide the first COI and CYTB gene sequences for this species in the entire Atlantic Forest. Phylogenetic analyses reveal that the generated sequences were recovered in the same clade as the published sequences of D. gnoma from the Amazonian Forest, corroborating the disjunct distribution pattern between Amazonia and PEC, as already reported for other vertebrate taxa. Also, this record increases the number of bat species to 71 for the PEC and extends the geographic distribution of D. gnoma by 758 kilometers to the northeast of its range.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240102"},"PeriodicalIF":1.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Academic impact and scientific quality should not be conflated. 学术影响力和科学质量不应混为一谈。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240738
Olavo B Amaral
{"title":"Academic impact and scientific quality should not be conflated.","authors":"Olavo B Amaral","doi":"10.1590/0001-3765202420240738","DOIUrl":"https://doi.org/10.1590/0001-3765202420240738","url":null,"abstract":"","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20240738"},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive assessment of the health condition of managed honey bees potentially exposed to contamination with agrochemical residues. 全面评估可能受到农用化学品残留污染的人工饲养蜜蜂的健康状况。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420231397
Marnix Doorn, Mayda Verde-Jiménez, Leslie Vallejos-Farías, Claudia Heredia-Yacila, Víctor R Abad-Pozo, Verónica R Olate-Olave
{"title":"Comprehensive assessment of the health condition of managed honey bees potentially exposed to contamination with agrochemical residues.","authors":"Marnix Doorn, Mayda Verde-Jiménez, Leslie Vallejos-Farías, Claudia Heredia-Yacila, Víctor R Abad-Pozo, Verónica R Olate-Olave","doi":"10.1590/0001-3765202420231397","DOIUrl":"https://doi.org/10.1590/0001-3765202420231397","url":null,"abstract":"<p><p>The growing demand for honey bee pollination services induces fluctuations in their populations and changes in bee health status. The current study aims to characterize the health condition of managed honey bees in three territories of the Department of Lambayeque, Peru, from a holistic perspective, considering several factors affecting bee health, including management practices, traits of the beekeepers and their apiaries, colony strength, infestation rates by Varroa sp., and the presence of agrochemical residues in bee bread. The results showed changes in land use, with large areas of crops dependent on bee pollination, determining their exposition to agrochemical residues. The 67% of bee bread samples presented pesticide residues, mainly, chlorpyrifos, carbendazim, boscalid, and azoxystrobin. In addition, limited expertise on good management practices by the beekeepers (i.e., inadequate disinfection of beekeeping materials, unsatisfactory varroa mites' surveillance, lack of protein supplements, presence of sanitary gaps) leads to deficiencies in bee colony strength and a high Varroa sp. prevalence in the territory, altering the bee health condition. To the best of our knowledge, this article illustrates the impact of the strongly anthropized ecosystems found along Peru's coast's arid zone on managed populations of honey bees in that territory with a One Health perspective.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20231397"},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro induced cold memory fails to enhance embryogenic cultures development in Araucaria angustifolia. 体外诱导的冷记忆不能促进 Araucaria angustifolia 胚胎培养物的发育。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240574
Gabriel Antônio P DE Matos, Evelyn L Dos Santos, Yohan Fritsche, Thiago S Ornellas, Neusa Steiner, Valdir Marcos Stefenon
{"title":"In vitro induced cold memory fails to enhance embryogenic cultures development in Araucaria angustifolia.","authors":"Gabriel Antônio P DE Matos, Evelyn L Dos Santos, Yohan Fritsche, Thiago S Ornellas, Neusa Steiner, Valdir Marcos Stefenon","doi":"10.1590/0001-3765202420240574","DOIUrl":"10.1590/0001-3765202420240574","url":null,"abstract":"<p><p>This research aimed to study how exposing somatic embryos of Araucaria angustifolia to cold temperatures in vitro could enhance somatic embryogenesis. The somatic embryos were induced from immature zygotic embryos, grown for 120 days, and then subjected to 4.5°C for 0, 2, 4, 8, or 16 days. After the cold treatment, the embryogenic cultures were returned to 25±2°C for 20 days and examined using light microscopy and cytochemistry. It was found that the embryogenic cultures at the PEM III stage did not show further development, and the growth of the embryogenic masses was negatively affected in the longer-duration cold treatment. Overcoming these challenges in the somatic embryogenesis protocol for A. angustifolia requires further integrative investigations on epigenetic events driving the zygotic embryo development.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20240574"},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical evaluation of combined autologous Platelet-Rich Plasma and Volume-Controlled Ozone Therapy in Facial Rejuvenation: A randomized controlled Pilot Study. 自体富血小板血浆和容量控制臭氧疗法联合用于面部年轻化的临床评估:随机对照试验研究。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240402
Carla Camargo, Carla Tim, Cintia Cristina S Martignago, Ana Claudia M Renno, Paula Cassanelli E Silva, Ana Laura M DE Andrade, Jynani Pichara, Eliana Claudia P Morato, José Ricardo DE Souza, Lívia Assis
{"title":"Clinical evaluation of combined autologous Platelet-Rich Plasma and Volume-Controlled Ozone Therapy in Facial Rejuvenation: A randomized controlled Pilot Study.","authors":"Carla Camargo, Carla Tim, Cintia Cristina S Martignago, Ana Claudia M Renno, Paula Cassanelli E Silva, Ana Laura M DE Andrade, Jynani Pichara, Eliana Claudia P Morato, José Ricardo DE Souza, Lívia Assis","doi":"10.1590/0001-3765202420240402","DOIUrl":"10.1590/0001-3765202420240402","url":null,"abstract":"<p><p>Platelet rich plasma and ozone therapy have been suggested in the clinical setting as promising and adjuvant therapies for managing the symptoms related to the facial aging. However, there is a lack of evidence about the effects of combining both therapies on rejuvenation. Thus, the aim of the present study was to evaluate and to compare the safety and efficacy of both therapies in facial aging. A single-center prospective pilot study was conducted. Female participants, aged 40 to 60 years, were randomized into 2 groups: participants received the platelet rich plasma treatment (PRP); participants received the therapy with platelet rich plasma associated with ozone therapy (PRPOT). Interventions were performed using the facial intradermal technique in 42 pre-established points, for 4 sessions with an interval of 15 days between them. After analyses performed using photographic records, assessment of frontal, periorbital, nasolabial and marionette wrinkles (Wrinkle Assessment Scale), self-esteem assessment (Brazilian Version of Rosenberg's Self-Esteem Scale) and assessment of patient satisfaction (FACE-Q) can be suggested that the platelet rich plasma protocol performed in the present study, as well as its association with volume-controlled ozone therapy, proved to be safe and potentially promising modalities in the treatment of facial aging.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20240402"},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of (-)-Fenchone antimicrobial activity against oral Candida albicans and toxicological parameters: an in silico, in vitro and ex vivo study. 评估(-)-葑酮对口腔白色念珠菌的抗菌活性和毒理学参数:一项体内、体外和体外研究。
IF 1.1 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.1590/0001-3765202420240273
André A Dos Santos, Abrahão A DE Oliveira-Filho, Bráulio A Teixeira, José Lucas F M Galvão, Maria Alice A DE Medeiros, Millena S Alves, David Henrique X Barbosa, Rodrigo P Mafra, Ulrich Vasconcelos, Edeltrudes O Lima
{"title":"Evaluation of (-)-Fenchone antimicrobial activity against oral Candida albicans and toxicological parameters: an in silico, in vitro and ex vivo study.","authors":"André A Dos Santos, Abrahão A DE Oliveira-Filho, Bráulio A Teixeira, José Lucas F M Galvão, Maria Alice A DE Medeiros, Millena S Alves, David Henrique X Barbosa, Rodrigo P Mafra, Ulrich Vasconcelos, Edeltrudes O Lima","doi":"10.1590/0001-3765202420240273","DOIUrl":"10.1590/0001-3765202420240273","url":null,"abstract":"<p><p>Candida albicans is the primary species causing oral candidiasis. Its increasing drug resistance drives the search for more effective antifungal agents. Therefore, we assessed toxicological parameters and the antimicrobial activity and mechanisms of action of the monoterpene (-)-fenchone against oral C. albicans. We conducted an in silico study using PASS online and AdmetSAR, followed by evaluation of antifungal activity through Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), association study with miconazole, and assays with sorbitol and ergosterol. Inhibition of biofilm formation and disruption of preformed biofilm were considered. Toxicity was also assessed through hemolysis assay. The in silico study revealed a higher likelihood of the compound being active for antifungal activity, as well as promising pharmacokinetic and toxicity characteristics. Subsequently, (-)-fenchone exhibited predominantly fungicidal activity (MIC90 = 8 μg/mL; MFC = 16 μg/mL), including against miconazole-resistant C. albicans isolates. The substance does not appear to act by damaging the fungal cell wall or plasma membrane, and exhibited synergy with miconazole. There was activity in inhibiting biofilm formation but not in disrupting preformed biofilm. Finally, the product exerted low hemolytic activity at more than MIC×10. Based on these results, (-)-fenchone may represent a promising therapeutic alternative for oral candidiasis.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20240273"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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