María Gabriela Romagnoli, Myriam Del Valle Catania, Marcelo Daniel Arana, Patricia Liliana Albornoz
{"title":"Ectomycorrhizas in Lycopodiopsida: their first registry and arbuscular mycorrhiza in Phlegmariurus saururus (Huperziaceae).","authors":"María Gabriela Romagnoli, Myriam Del Valle Catania, Marcelo Daniel Arana, Patricia Liliana Albornoz","doi":"10.1590/0001-3765202520241248","DOIUrl":"https://doi.org/10.1590/0001-3765202520241248","url":null,"abstract":"<p><p>Lycophytes show no instances of ectomycorrhizas. Phlegmariurus saururus is the only Huperziaceae that grows in Comechingones biogeographic province, in sunny, exposed surficial rock crevices with poor soil development and very scarce water. As mycorrhizas improve plant fitness in natural ecosystems, it was hypothesized that P. saururus can develop multiple types of fungal symbiosis, including ectomycorrhizas. For detecting, identification and description of mycorrhizas, conventional techniques were employed, and samples of roots were cut with an ultra-microtome to obtain thin (3 µm) and ultrathin (0.1 µm) sections. Phlegmariurus saururus is the first species of Lycopodiopsida where the ectomycorrhizas are evidenced. Arbuscular mycorrhizas and septate endophytes were also found. Ectomycorrhizas can alter the anatomy and hydrophilic properties of roots, improving the adaptation of the plant hosts to habitats with a marked period of drought, as the novel Andean Comechingones habitats. The ectomycorrhizas detected in P. saururus could be considered as an adaptive mechanism related to the successful colonisation of this habitat and can undergo a significant transformation in the lifestyle of fungal symbiosis of lycophytes, which could provide important insights into this morphological and functional evolution.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241248"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laiza Camila O Mateus, José Raimundo Correa, Patrícia Elaine DE Almeida
{"title":"Extracellular Vesicles in COVID-19: Mechanistic insights and emerging therapeutic strategies.","authors":"Laiza Camila O Mateus, José Raimundo Correa, Patrícia Elaine DE Almeida","doi":"10.1590/0001-3765202520241216","DOIUrl":"https://doi.org/10.1590/0001-3765202520241216","url":null,"abstract":"<p><p>The COVID-19 pandemic has profoundly impacted global health, driven in part by the high mutation rate of SARS-CoV-2, which has resulted in the emergence of variants with enhanced transmissibility, virulence, and immune evasion capabilities. This evolving threat highlights the urgent need for innovative therapeutic strategies, as current treatments have shown limited efficacy. In this context, Extracellular Vesicles (EVs) present a promising alternative. EVs play a crucial role in intercellular communication and offer advantages due to their biocompatibility and mechanisms of cellular entry as viruses, making them an important tool against SARS-CoV-2. One of the principal immune evasion strategies employed by SARS-CoV-2 involves the release of EVs capable of transporting viral antigens. Numerous studies have suggested the role of exosomes and microvesicles as carriers of viral components, indicating that EVs can be targets in the progression of infection. Exosomes derived from infected cells could function as biomarkers and as potential drug delivery vehicles. Furthermore, EVs play a regulatory role in immune responses, facilitating cytokine production and antigen presentation. This review aims to elucidate the molecular mechanisms underlying the involvement of EVs in SARS-CoV-2 infection, examine their implications for biomarker development and explore their potential therapeutic applications in COVID-19.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241216"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roberto F Manghi, Érica V Nogueira, Audrey Helen M A Cysneiros
{"title":"Local influence diagnostics in elliptical multilevel models.","authors":"Roberto F Manghi, Érica V Nogueira, Audrey Helen M A Cysneiros","doi":"10.1590/0001-3765202520240796","DOIUrl":"https://doi.org/10.1590/0001-3765202520240796","url":null,"abstract":"<p><p>Local influence diagnostics for elliptical multilevel models are proposed in this paper. This class of models involves the use of continuous symmetric distributions, including heavy-tailed error models, and the normal distribution as a particular case. Maximum likelihood estimation and features related to the fitting and model assumptions are discussed. Finally, local influence measures under different perturbation schemes are developed and applied to fit real data under the normal, Student- t and power exponential distributions.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240796"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thomas P Stephen Livingston, Petchimuthu Madhu, Chandrasekaran Sowmya Dhanalakshmi, Vadivel Ayyakkannu
{"title":"Non-catalytic and catalytic co-pyrolysis of neem seed cake and plastic waste: an experimental investigation on product distribution, synergistic interaction and characterization.","authors":"Thomas P Stephen Livingston, Petchimuthu Madhu, Chandrasekaran Sowmya Dhanalakshmi, Vadivel Ayyakkannu","doi":"10.1590/0001-3765202520241284","DOIUrl":"https://doi.org/10.1590/0001-3765202520241284","url":null,"abstract":"<p><p>This investigation elucidates the co-pyrolysis of neem seed cake in combination with plastic waste across a spectrum of mass ratios namely, 0:1, 3:1, 2:1, 1:1, 1:2, 1:3, and 1:0 subjected to varying pyrolytic temperatures from 350°C to 650°C, employing a CuO catalyst as a facilitating agent. The research concentrated on elucidating the effect of reaction temperature and the blend ratio of neem seed cake to plastic waste on the distribution of products and the chemical composition of the resultant pyrolysis oil. The co-pyrolysis performed at a 1:2 ratio of neem seed cake to plastic waste yielded an optimal oil production of 69.4 wt% with maximum positive synergy of 6.25% at 500°C. The physicochemical characteristics of the resulting co-pyrolysis oil demonstrated a striking resemblance to those of conventional fossil diesel. Further analysis through FT-IR revealed the presence of a different range of aromatic components. Quantitative analysis utilizing chromatographic peak area evaluations was undertaken to elucidate the compositional profile of the pyrolysis oils, thereby accentuating the presence of synergistic effects. Furthermore, GC-MS analysis provided empirical validation of the interaction between neem seed cake and waste plastics during co-pyrolysis, as indicated by discernible decrease in the concentration of oxygenated compounds.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241284"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Sol Terlizzi, Mariano Zukerfeld, Fernanda Beigel
{"title":"Open access, \"piracy\" and Article Processing Charges (APC) in Argentina: an informed policy for the national research funding agency.","authors":"María Sol Terlizzi, Mariano Zukerfeld, Fernanda Beigel","doi":"10.1590/0001-3765202520241184","DOIUrl":"https://doi.org/10.1590/0001-3765202520241184","url":null,"abstract":"<p><p>The article explores the growing influence of Article Processing Charges (APCs) in academic publishing, especially in Argentina, and the challenges they pose for non-hegemonic countries. It highlights the shift from traditional subscription models to open access models, driven by commercial publishers, which often impose significant financial burdens on researchers and institutions. The study aims to examine the issues arising from these models, particularly the commercial open access system, to describes the actions developed by the National Agency for the promotion of Research, Technological Development and Innovation from Argentina, and to discuss some public policy proposals. One major finding is that APCs can exacerbate global inequalities in scientific publishing, as researchers from middle- and low-income countries struggle to afford these fees. This trend can distort research agendas and limit international collaborations. Besides, public research funding agencies play a crucial role in shaping publishing practices, and thus have the power to either promote or hinder more equitable publishing models. The article concludes that non-commercial open access routes, like the \"diamond\" model, should be promoted, and research assessments should shift away from focusing on journal rankings to encourage more responsible research dissemination. The study calls for reforms in both national and global publishing policies.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241184"},"PeriodicalIF":1.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo José DE Arruda, Cícera Maria DA Silva, Alessandra R Lima, Glenda B Porzani, Monize M DA Silva, Cláudio T DE Carvalho, Isaías Cabrini, Magda F Fernandes
{"title":"Toxicity of copper (II) metal complexes with amino acids ligands: A study of activity in Aedes aegypti larvae.","authors":"Eduardo José DE Arruda, Cícera Maria DA Silva, Alessandra R Lima, Glenda B Porzani, Monize M DA Silva, Cláudio T DE Carvalho, Isaías Cabrini, Magda F Fernandes","doi":"10.1590/0001-3765202520240842","DOIUrl":"https://doi.org/10.1590/0001-3765202520240842","url":null,"abstract":"<p><p>The larval control of Aedes aegypti has been done mainly by insecticides, which cause resistance and environmental contamination and have a high-cost development and application. The need for alternative solutions to problems caused by synthetic insecticides has led to studies to find new, active, and less toxic compounds, such as metallo-insecticides. In the present study, the toxic activity of Cu(II) - complexes with the amino acids tryptophan, histidine, methionine, and taurine in Ae. aegypti larvae were evaluated. The Cu(II) - histidine complex showed no toxicity. For the Cu(II) - tryptophan and Cu(II) - methionine complexes, the mortality percentages ranged from 3.16% to 46.55% and 5.46% to 12.42%, respectively. Cu(II) - taurine presented 100% mortality in 24 h at the lowest concentration tested (50 mg L-1, ppm). Considering the concentration range of 50 to 1000 mg L-1 and 24 h of exposure of the larvae to the metal complexes, the series of decreasing toxicity is Cu(II) - taurine > Cu(II) - tryptophan > Cu(II) - methionine > Cu(II) - histidine. From these studies, the Cu (II) - taurine complex can be a candidate to be used as an alternative and less toxic insecticide, low-cost for control of Ae.aegypti larvae.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240842"},"PeriodicalIF":1.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucimara C DE Souza, Maria Amélia G Ferrão, Aymbiré Francisco A DA Fonseca, Paulo Sérgio Volpi, Marcone Comerio, Lucas L Pereira, José Augusto M Carvalho, Sérgio H Saraiva, Patrícia F Pinheiro, Adelson L DA Silva Júnior, Taís Cristina B Soares
{"title":"An interdisciplinary approach for evaluating beverage quality in Coffea canephora.","authors":"Lucimara C DE Souza, Maria Amélia G Ferrão, Aymbiré Francisco A DA Fonseca, Paulo Sérgio Volpi, Marcone Comerio, Lucas L Pereira, José Augusto M Carvalho, Sérgio H Saraiva, Patrícia F Pinheiro, Adelson L DA Silva Júnior, Taís Cristina B Soares","doi":"10.1590/0001-3765202520240084","DOIUrl":"https://doi.org/10.1590/0001-3765202520240084","url":null,"abstract":"<p><p>The objective of this study was to assess the quality of Conilon coffee (Coffea canephora) based on agronomic, physicochemical, sensory profiles, and molecular markers. The dataset comprises 107 genotypes from the Incaper breeding program, evaluated in Marilândia, ES, Brazil. A total of 30 traits and 14 SSR markers were examined, revealing significant genetic variability. Most genotypes exhibited a medium June harvest cycle, uniform ripening, medium-sized beans, high processing yield, and a high percentage of flat and peaberry beans. Physicochemical variables, total titratable acidity, and potassium leaching contributed significantly to the observed variability. Chlorogenic acids (4.82%) and caffeine (2.58%) were the most abundant bioactive compounds. In sensory evaluations, 34 genotypes received scores exceeding 80. 13 markers were recommended for future association mapping studies to identify QTLs influencing the traits evaluated. Results identified genotypes with potential to maximize heterosis and genetic diversity in advancing generations. Among the evaluated genotypes, 34 were identified as promising due to their high sensory scores (≥ 80 points) and favorable agronomic, physicochemical, sensory, and genetic traits. The progeny HS17 stood out as the most divergent in agronomic and genetic traits compared to the other genotypes.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240084"},"PeriodicalIF":1.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diego Nogare, Ismar F Silveira, Renato Banzai, Maína C Alexandre
{"title":"Make or buy strategy for Machine Learning Operations - MLOps.","authors":"Diego Nogare, Ismar F Silveira, Renato Banzai, Maína C Alexandre","doi":"10.1590/0001-3765202520240924","DOIUrl":"https://doi.org/10.1590/0001-3765202520240924","url":null,"abstract":"<p><p>This research addresses the make or buy strategy for Machine Learning Operations (MLOps), exploring the decision between developing internally or purchasing computational solutions for Machine Learning projects. Considering factors such as cost, quality, technical expertise and strategic alignment, organizations face the challenge of balancing product complexity, core competencies and risk management. This research highlights the importance of understanding the needs of each project when analyzing existing offers to solve problems and maintain competitiveness in the market, offering a guide for drive and support your decision. Additionally, qualitative and quantitative reviews of MLFlow, Airflow, Kubeflow, Databricks, Dataiku, H2O, Amazon AWS, Microsoft Azure, and Google GCP tools are presented, which facilitate the life-cycle management of machine learning models. This research contributes to the understanding of the challenges and strategies involved in the effective implementation of MLOps projects.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240924"},"PeriodicalIF":1.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A new ornithocheiran pterosaur from the Upper Cretaceous (Cenomanian) of Saratov, Russia.","authors":"Alexander O Averianov","doi":"10.1590/0001-3765202520241063","DOIUrl":"https://doi.org/10.1590/0001-3765202520241063","url":null,"abstract":"<p><p>Saratovia glickmani, gen. et sp. nov., is described based on a dentary symphysis fragment from the Upper Cretaceous (upper Cenomanian) Melovatka Formation at Saratov, Russia. This specimen was found by L.S. Glickman in late 1940s and was referred previously to Ornithocheirus, Anhanguera, or Coloborhynchus. S. glickmani is attributed to Targaryendraconia because of strongly scalloped and subparallel lateral margins of dentary symphysis, which is transversely narrow, with the interalveolar width subequal to the alveolar width. It differs from all known targaryendraconians by having a dentary crest. The new taxon differs from all toothed pterodactyloids by lack of median groove on dentary symphysis. Instead, it has a raised flat platform pierced by numerous relatively large nutrient foramina, which are connected by short canals with a median canal within this platform. S. glickmani is one of the latest toothed pterosaurs known in the fossil record.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97Suppl. 1 Suppl. 1","pages":"e20241063"},"PeriodicalIF":1.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Valeska M Scuro, Augusto C Dotta Filho, Daniel Rapachi, Ana C Mazzocato, Marcos A Gelesky, Flávio A Pavan
{"title":"Novel biosynthesis of silver nanoparticles catalytically active using extract of non- native species (Eragrostis plana Nees) of Brazilian Pampa.","authors":"Valeska M Scuro, Augusto C Dotta Filho, Daniel Rapachi, Ana C Mazzocato, Marcos A Gelesky, Flávio A Pavan","doi":"10.1590/0001-3765202520241276","DOIUrl":"https://doi.org/10.1590/0001-3765202520241276","url":null,"abstract":"<p><p>Novel silver nanoparticles (AgNPs) were synthesized using biomass from Eragrostis plana Nees, commonly known as Tough lovegrass, an invasive grass species in the Brazilian Pampa. AgNPs were synthesized in a one-step synthesis using the extract as a reducing agent for Ag+ ions and stabilizing agent. The results showed that the colloidal solution of AgNPs had a surface plasmon resonance (SPR) band at 410 nm. Through vibrational analysis, they showed that the OH, NH, CONH2, COC, COOH, and CO present in the extract were associated with reducing Ag+ ions. The synthesized nanoparticles were crystalline and, through morphological analysis, showed that the AgNPs produced had different shapes, with an average diameter of 23 ± 8 nm. The biosynthesized AgNPs exhibited high catalytic activity for reducing Toluidine blue (TB). The total reduction of TB with AgNPs in the presence of NaBH4 at 25 °C occurred after only 5 min of reaction.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20241276"},"PeriodicalIF":1.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}