Raquel Andressa Brito, Carla A Silva, Andre C Amaral
{"title":"Design, preparation, and characterization of bovine papillomavirus chitosan-based nanoparticles as a prototype for a bovine papillomatosis vaccine.","authors":"Raquel Andressa Brito, Carla A Silva, Andre C Amaral","doi":"10.1590/0001-3765202520241155","DOIUrl":"10.1590/0001-3765202520241155","url":null,"abstract":"<p><p>The bovine papillomavirus (BPV) is known to cause lesions in the epithelial tissue of cattle, which causes bovine papillomatoses. This disease is characterized by wart lesions in different body parts, causing losses in the animal value. Despite the economic impact, there are no effective vaccines available at this moment. The present study aims to develop a nanoformulated autogenous vaccine using the inactivated virus present in warts to control the diseases associated with BPV. To this end, papillomavirus was collected from infected bovines on a farm in Goiás, Brazil. The warts containing the viruses were macerated to obtain the inactivated viruses to be formulated within chitosan nanoparticles. The chitosan nanoparticles containing the inactivated viruses presented 330.7 nm in size, a polydispersity index of 0.41, and a positive Zeta potential of 38.2 mV. The association efficiency was 58.3%. The nanoparticles containing the inactivated virus were stable for up to 30 days and presented no hemolysis toxicity. This could be an interesting approach to develop a vaccine to protect bovines from BPV infection.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20241155"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carine Dahl Corcini, Antonio Sergio Varela Junior, Marc Yeste
{"title":"Environmental contamination and male reproductive health: (ir) reversible effects in child- and adulthood.","authors":"Carine Dahl Corcini, Antonio Sergio Varela Junior, Marc Yeste","doi":"10.1590/0001-3765202520240717","DOIUrl":"10.1590/0001-3765202520240717","url":null,"abstract":"<p><p>Infertility affects 10-15% of reproductive-age couples, with causes ranging from genetic factors to unidentified reasons. Environmental conditions, particularly pollutants, play a significant role in male fertility. Yet, public health policies often overlook reproductive health, despite mounting evidence of pollutants' detrimental repercussion. Understanding this impact is crucial to prevent the effects of dangerous exposure, especially given the high levels of environmental pollutants in today's world. Most of the previous research about the adverse effects from contaminants has been conducted in rodents, with limited human epidemiological research. This article reviews the evidence about the impact of various contaminants (air pollutants, water contaminants, pesticides, herbicides, radiation, heavy metals, and plastics) on male reproductive health, particularly sperm quality and fertility. The literature suggests that exposure to contaminants during fetal development and childhood has irreversible effects, while those of adult exposure are often reversible. These findings highlight the need to alert society about reproductive health threats from certain contaminants. Public authorities should consider this situation when designing health plans, and individuals envisaging fatherhood should be aware of these risks.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240717"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Silvia Conceição R P Mello, Rossiane M DE Souza, Eliane Rodrigues, Fernando David Caracuschansk, Beatriz V Mendes, Eliana DE Fátima M DE Mesquita
{"title":"Salmonella spp. detection in fresh and freeze-dried frog meat: a comparison of methods.","authors":"Silvia Conceição R P Mello, Rossiane M DE Souza, Eliane Rodrigues, Fernando David Caracuschansk, Beatriz V Mendes, Eliana DE Fátima M DE Mesquita","doi":"10.1590/0001-3765202520230552","DOIUrl":"10.1590/0001-3765202520230552","url":null,"abstract":"<p><p>This study examined the presence of Salmonella spp. in fresh frog meat and freeze-dried seasoned frog meat stored for up to 90 days at room temperature. The samples were analyzed in triplicate using the conventional official method, by polymerase chain reaction (PCR) and mass spectrometry technique (MALDI -TOF) - for a comparison of the results obtained by the three tested methods. Mass spectrometry analyses confirmed the presence of Salmonella spp. in 100% of the fresh frog meat samples. In the analysis by the conventional method, the percentages of detection were 67% and 100% in samples B2 and B3, whereas the PCR method indicated 100% and 67% presence of the bacteria in the respective samples. In the analyses of freeze-dried none of the three methods detected the presence of Salmonella spp. The different analysis methods tested in this study were efficient in determining the presence or absence of Salmonella spp. in the samples, and the comparison between methods revealed a high percentage of compatibility of results. Adoption of efficient detection methods, such as PCR and MALDI-TOF, is essential, not only for public health, but also for compliance with Brazilian regulations, ensuring that food products are free from Salmonella spp. contamination and safe for consumption.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20230552"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rayane Duarte, Bruna R Martinatti, Viviane M DE Lima, Rafael A Tubino, Mariana S Oliveira, Bruno P Berto, Michelle Daniele Santos-Clapp
{"title":"Contracaecum jorgei in common snooks in Brazil: new host, locality record and remarks on misidentifications of Contracaecum spp. in GenBank.","authors":"Rayane Duarte, Bruna R Martinatti, Viviane M DE Lima, Rafael A Tubino, Mariana S Oliveira, Bruno P Berto, Michelle Daniele Santos-Clapp","doi":"10.1590/0001-3765202520240332","DOIUrl":"10.1590/0001-3765202520240332","url":null,"abstract":"<p><p>Contracaecum jorgei was recently described as a new anisakid species from the Argentine wolffish Hoplias argentinensis. This study aimed to report a new host and locality for C. jorgei and highlight inconsistencies in some Contracaecum spp. deposits based on sequence comparisons. Through morphometric analyses and comparative analyses of genetic sequences in GenBank, using a locus from the cytochrome oxidase subunit 2 gene, present specimens were identified as C. jorgei. Misidentified sequences of Contracaecum spp. were also observed and discussed. Thus, C. jorgei is reported for the first time in the Brazilian common snook Centropomus undecimalis, expanding the reports and, consequently, its geographic distribution in the Neotropical region.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240332"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reinaldo Y Morita, Juliana R Kloss, Maria Izabel X Scapolan, Ronilson V Barbosa, Ana Lúcia N DA Silva
{"title":"Evaluation of using poly(ethylene-co-vinyl acetate)/organoclays nanocomposites in blown film production.","authors":"Reinaldo Y Morita, Juliana R Kloss, Maria Izabel X Scapolan, Ronilson V Barbosa, Ana Lúcia N DA Silva","doi":"10.1590/0001-3765202520230781","DOIUrl":"10.1590/0001-3765202520230781","url":null,"abstract":"<p><p>Poly(ethylene-co-vinyl acetate)/organoclay (EVA/OC) systems were prepared by melt mixing in a twin-screw extruder. The potential application of the nanocomposites in the production of blow molding films was analyzed by mechanical, thermal, morphological, X-ray diffraction, water vapor permeability and rheological properties. A modified organoclay (OC) with a non-ionic surfactant, free of ammonium salt (Vulgel-AM), and an organoclay modified with cetyltrimethylammonium bromide (Vulgel-CT) were added in EVA matrix (1.5 wt%). The X-ray diffraction (XRD) showed that EVA/Vulgel-AM presented a partially exfoliated morphology. The EVA/Vulgel-AM when compared to EVA/Vulgel-CT system presented higher toughness property, higher thermal stability, a more pronounced water vapor barrier behavior and appropriate balance of viscoelastic in rheological properties. Finally, the results of the analysis performed in this study it is signaled that EVA/Vulgel-AM is a more viable and interesting candidate to produce a stable blow film when compared to EVA/Vulgel-CT.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20230781"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Caroline Valente, Marcos L Neves, Scheila I Kraus, Morgana D DA Silva
{"title":"Vagus nerve stimulation -induced anxiolytic and neurotrophic effect in healthy rats.","authors":"Caroline Valente, Marcos L Neves, Scheila I Kraus, Morgana D DA Silva","doi":"10.1590/0001-3765202520241292","DOIUrl":"10.1590/0001-3765202520241292","url":null,"abstract":"<p><p>Memory, learning, anxiety, and depression have concerned science for years, increasingly leading to research into new therapeutic targets. The study investigated the effect of acupuncture stimulation of the auricular branch of the vagus nerve (ABVN) on memory, anxiety-like and depression-like behaviors in healthy rats. Healthy rats were divided into groups: (1) control, without treatment; (2) acupuncture in the left ABVN; and (3) acupuncture in the right ABVN. Tests were conducted to evaluate memory (inhibitory avoidance and object recognition), anxiety-like (open field, elevated plus maze, and light/dark box), and depressive-like (sucrose preference and forced swimming) behaviors. In addition, the concentration of brain-derived neurotrophic factor (BDNF) in the hippocampus of the rats was evaluated. The control group did not show any changes in the behavioral tests. The animals that received acupuncture (ABVN-R and ABVN-L) remained longer on the light side of the light/dark box test compared to the control group. Acupuncture in the ABVN-R also increased the concentration of BDNF in the hippocampus of the animals. Accordingly, acupuncture stimulation of the ABVN did not show an antidepressant effect or improve memory in healthy animals; however, it did show an anxiolytic effect and increased neurotrophic levels in the hippocampus.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20241292"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcos Jorge M Dubeux, Ubiratã F Souza, Ricardo Lourenço-DE-Moraes, Gentil A Pereira-Filho, Raul F D Sales, Fagner R Delfim, Frederico Gustavo R França, Marco Antônio DE Freitas, Robson Waldemar Ávila, Marília B Lion, Eliza Maria X Freire, Selma Torquato, Geraldo Jorge B DE Moura, Adrian Antonio Garda, Daniel O Mesquita, Tamí Mott, Pedro M Sales Nunes
{"title":"Amphisbaenians and lizards of the Pernambuco Endemism Center: species richness, sampling gaps, and conservation status in one of the most threatened regions of the Atlantic Forest.","authors":"Marcos Jorge M Dubeux, Ubiratã F Souza, Ricardo Lourenço-DE-Moraes, Gentil A Pereira-Filho, Raul F D Sales, Fagner R Delfim, Frederico Gustavo R França, Marco Antônio DE Freitas, Robson Waldemar Ávila, Marília B Lion, Eliza Maria X Freire, Selma Torquato, Geraldo Jorge B DE Moura, Adrian Antonio Garda, Daniel O Mesquita, Tamí Mott, Pedro M Sales Nunes","doi":"10.1590/0001-3765202520240284","DOIUrl":"10.1590/0001-3765202520240284","url":null,"abstract":"<p><p>In this study, we integrated data from more than 30 years of inventories available in the main herpetological collections of the Pernambuco Endemism Center (PEC), northern Atlantic Forest. We aimed to reduce the Wallacean shortfall of amphisbaenians and lizards (non-snake squamates) in the region, to provide distribution maps of all species, and to direct future survey efforts and conservation strategies in one of the most threatened portions of the Atlantic Forest. The species list was based exclusively on records associated with vouchers from localities in the PEC and transition zones. We recorded 50 species of non-snake squamates in the PEC, distributed across 97 municipalities in four states. Among the species registered in the PEC, seven are topotypical, five are endemic and three are considered threatened with extinction. Only 35% of the municipalities in the PEC have at least one individual deposited in the herpetological collections analyzed and of these only 35% have more than ten species registered. The most representative areas are concentrated in the coastal portion of the PEC, and arranged in four main zones. These zones mainly overlap with areas close to state capitals and around some of the PEC's protected areas.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240284"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Márcia S Coelho, Lúcia Helena P Kiill, Celso F Martins
{"title":"Efficiency of pantraps for monitoring bees diversity in Brazilian acerola orchards: the role of color diversity.","authors":"Márcia S Coelho, Lúcia Helena P Kiill, Celso F Martins","doi":"10.1590/0001-3765202520240521","DOIUrl":"10.1590/0001-3765202520240521","url":null,"abstract":"<p><p>Bee monitoring characterizes the local fauna and determines conservation measures. The study evaluated the influence of pantrap color on bee attraction and the difference in seasonal patterns of these insects. Collections were carried out in commercial plantations of Malpighia emarginata in the Northeast region of Brazil, using yellow, blue and white pantraps. A total of 1,449 bee specimens belonging to 59 species, four subfamilies of Apidae, 18 tribes and 31 genera were captured, with emphasis on the subfamily Apinae (89.6%). Blue pantraps captured 66.5% of the total sampled specimens, followed by white (19.6%) and yellow (13.9%). Melitomella grisescens (29.3%) was the most abundant species, followed by Apis mellifera (10.3%), Melitoma segmentaria (10.1%), Ptilothrix plumata (9.6%) and Melitoma ipomoearum (6.8%). With the exception of A. mellifera, all the most abundant species belonged to the Emphorini tribe (56%). Regarding the Centridini tribe, pantraps were efficient in collecting species, but not individuals. The months of September and November/2019 were the months when the lowest numbers of insects were collected and the lowest rainfall rates were recorded. In this sense, understanding new methodologies becomes essential to identify the diversity of pollinators for the construction of management and conservation plans.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240521"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sidney S Silva, Imaculada M C Ananias, André S Souza, Fábio Aremil C Santos, Naiara Melo, Luis David S Murgas, Ronald K Luz
{"title":"Physiological and oxidative responses of Colossoma macropomum and hybrid ♀ C. macropomum × ♂ Piaractus brachypomus subjected to different stressors in a recirculating aquaculture system (RAS).","authors":"Sidney S Silva, Imaculada M C Ananias, André S Souza, Fábio Aremil C Santos, Naiara Melo, Luis David S Murgas, Ronald K Luz","doi":"10.1590/0001-3765202520240645","DOIUrl":"10.1590/0001-3765202520240645","url":null,"abstract":"<p><p>The effects of acute handling stress on the physiological and antioxidant responses of tambaqui (Colossoma macropomum) and the hybrid tambatinga (C. macropomum × Piaractus brachypomus) raised in a recirculating aquaculture system were investigated. Thirty juveniles of tambaqui (27.8 ± 2.8 g) and thirty of tambatinga (26.2 ± 3.1 g) were used. Six fish/group were captured and subjected to the sample to determine basal parameters. The remaining animals were chased with hand net for 3 minutes, exposed to air for 2 minutes and subjected to biometry. Blood and oxidative parameters were determined immediately after stress (IAE) and after 1h (1hAE), 24h (24hAE) and 48h (48hAE). Stress caused an increase in the hematocrit of tambaqui at IAE, with reduction after 48hAE. For glycemia, there were differences between the fish groups and collections with higher values for tambatinga and after IAE and 1hAE. Cholesterol in tambaqui increased after 24hAE and 48hAE, decreasing in hybrid after 24hAE with return to basal after 48hAE. SOD increased in IAE while in tambaqui the response only occurred after 48hAE, resulting in an increase in lipid peroxidation. Conclude that biometric management resulted in hemodilution in tambatinga, without recovery of basal parameters. However, the antioxidant system prevented lipid peroxidation.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240645"},"PeriodicalIF":1.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariana P C Cibreiros, Marnie Hillary C Leão, Claudia Mermelstein, Manoel Luis Costa
{"title":"Show me the image: a systematic analysis on how results are represented in publications from different fields of biomedical and biological research.","authors":"Mariana P C Cibreiros, Marnie Hillary C Leão, Claudia Mermelstein, Manoel Luis Costa","doi":"10.1590/0001-3765202520241023","DOIUrl":"10.1590/0001-3765202520241023","url":null,"abstract":"<p><p>Figures are essential to convey the main results of scientific articles. Different biomedical research fields have different methodologies and therefore different forms of data representation. To understand whether there are distinct patterns of data representation, we analyzed how results are displayed in scientific publications from six fields: Biochemistry and Cell Biology, Bioinformatics and Computational Biology, Clinical Sciences, Oncology and Carcinogenesis, Pharmacology and Pharmaceutical Sciences, and Zoology. Our results show that Graphics were the most frequent type of representation, followed by Schemes and diagrams. Microscopy was the third most used type of image in most fields, except in Pharmacology and Pharmaceutical Sciences, where Molecules and chemical reactions were the third most frequent. Interestingly, each research field has a characteristic pattern of image. We further classified the image types in primary or secondary data, according to the level of human interference in its construction. Each field has a particular proportion of primary and secondary images. We also analyzed the frequency of words and observed a remarkable vocabulary difference between fields. The most frequent word of each field nicely correlates with the unique type of figures used. Specific fields might gain more visibility for their data by using diverse approaches in image representation.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20241023"},"PeriodicalIF":1.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}